Rw6G043870

kDa class II heat shock protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001449
Physical Location & Seq
Reverse (-)
67022068 .. 67022682
615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043870.1

Sequence Viewer

Length: 615 bp
ATGGACCTCATGATTCCCCTGAGGAACATGGGTGTCGACAGCAACCTCCTGGATGCCCTCCACGACCTTTTGGACTTCTCTGACGAGCAAAACCAGGCGAGCCACCATGCGCCTTCGCGCCAATATGTCCGGGAAGCCAAGGCCATGTCGGCTACTCCAGCTGACGTGAAGGAGACTCAGAATGCCTACATCTTTGTGATAGATGTGCCCGGGCTGAAGTCGGACCAGATCAAAGTCCATCTGGAAGACGACAACGTTTTGGTGGTGTGCGGCGAGAGGAAGAGGGAGAAGGAGAGAGACAAGGGAGTGAGGTACCTGAGGATGGAGAGGAGGCTCGGCAAGTATCTCAAGAAGTTTGTGCTGCCGGAGAATGCTGATGTAGAGAAGATCAGTGCCGAGTGTCAGGATGGGGTGTTGACTATTGTGGTGGAGAAGAAGCCGCCGCCGGAGCTGAAGAAGCCCAAGATTGTTCAGGTGAGGATTGGCGACCGCCAAGAAGGCCAAGAGGGTCAAGGTGGCCAAGAGGGTCAAAGTGGAGGTCAAGGAGACCAGTGGGGTCAAAAGGGCCAAGGAGGTCAAGAGGGTCAGGGTGGCCAAGATGGCAACAGCCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

204

Amino Acids

22.69

Weight (kDa)

5.67

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 55 - 159 9.1e-27 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 36
AccII CGCG 1 cut(s) 118
AciI CCGC 5 cut(s) 270, 440, 443, 490, 610
AclI AACGTT 1 cut(s) 255
AcoI YGGCCR 2 cut(s) 517, 592
AcuI CTGAAG 2 cut(s) 236, 473
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 322
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 2 cut(s) 48, 93
AluBI AGCT 2 cut(s) 161, 451
AluI AGCT 2 cut(s) 161, 451
Alw26I GTCTC 3 cut(s) 167, 291, 540
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 5 cut(s) 141, 499, 517, 565, 592
ApeKI GCWGC 1 cut(s) 361
ArsI GACNNNNNNTTYG 2 cut(s) 523, 555
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 2 cut(s) 112, 120
AspS9I GGNCC 3 cut(s) 4, 223, 565
AsuC2I CCSGG 3 cut(s) 131, 210, 211
AsuHPI GGTGA 1 cut(s) 487
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 2 cut(s) 4, 223
AxyI CCTNAGG 2 cut(s) 20, 317
BaeGI GKGCMC 1 cut(s) 210
BalI TGGCCA 2 cut(s) 519, 594
BanI GGYRCC 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 348
BccI CCATC 4 cut(s) 246, 316, 401, 593
BciT130I CCWGG 2 cut(s) 50, 95
BcnI CCSGG 3 cut(s) 131, 210, 211
BcoDI GTCTC 3 cut(s) 167, 291, 540
BfaI CTAG 1 cut(s) 613
BglI GCCNNNNNGGC 3 cut(s) 149, 498, 600
BisI GCNGC 5 cut(s) 271, 362, 440, 443, 610
BlsI GCNGC 5 cut(s) 272, 363, 441, 444, 611
Bme1390I CCNGG 5 cut(s) 50, 95, 131, 210, 211
Bme18I GGWCC 2 cut(s) 4, 223
BmeT110I CYCGRG 1 cut(s) 209
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 3 cut(s) 4, 223, 565
BmiI GGNNCC 1 cut(s) 314
BmrFI CCNGG 5 cut(s) 50, 95, 131, 210, 211
BmsI GCATC 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 3 cut(s) 131, 210, 211
BsaI GGTCTC 1 cut(s) 540
BsaJI CCNNGG 3 cut(s) 138, 209, 568
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse1I ACTGG 1 cut(s) 550
Bse21I CCTNAGG 2 cut(s) 20, 317
BseBI CCWGG 2 cut(s) 50, 95
BseDI CCNNGG 3 cut(s) 138, 209, 568
BseGI GGATG 3 cut(s) 58, 327, 412
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMII CTCAG 3 cut(s) 11, 191, 308
BseNI ACTGG 1 cut(s) 550
BseRI GAGGAG 1 cut(s) 343
BseSI GKGCMC 1 cut(s) 210
BseXI GCAGC 1 cut(s) 348
Bsh1236I CGCG 1 cut(s) 118
Bsh1285I CGRYCG 1 cut(s) 490
BshFI GGCC 5 cut(s) 143, 501, 519, 567, 594
BshNI GGYRCC 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 490
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 4 cut(s) 130, 210, 365, 446
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 3 cut(s) 167, 291, 540
BsmI GAATGC 2 cut(s) 187, 376
BsnI GGCC 5 cut(s) 143, 501, 519, 567, 594
Bso31I GGTCTC 1 cut(s) 540
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 228, 387
BspACI CCGC 5 cut(s) 270, 440, 443, 490, 610
BspANI GGCC 5 cut(s) 143, 501, 519, 567, 594
BspCNI CTCAG 3 cut(s) 12, 190, 309
BspFNI CGCG 1 cut(s) 118
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 540
BsrI ACTGG 1 cut(s) 550
BssECI CCNNGG 3 cut(s) 138, 209, 568
BssMI GATC 2 cut(s) 228, 387
BssT1I CCWWGG 2 cut(s) 138, 568
Bst2UI CCWGG 2 cut(s) 50, 95
Bst6I CTCTTC 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 3 cut(s) 20, 177, 317
BstF5I GGATG 3 cut(s) 58, 327, 412
BstFNI CGCG 1 cut(s) 118
BstHHI GCGC 2 cut(s) 112, 120
BstKTI GATC 2 cut(s) 231, 390
BstMAI GTCTC 3 cut(s) 167, 291, 540
BstMBI GATC 2 cut(s) 228, 387
BstMCI CGRYCG 1 cut(s) 490
BstMWI GCNNNNNNNGC 7 cut(s) 149, 158, 448, 457, 498, 600, 609
BstNI CCWGG 2 cut(s) 50, 95
BstSCI CCNGG 5 cut(s) 48, 93, 129, 208, 209
BstSLI GKGCMC 1 cut(s) 210
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 348
BstV2I GAAGAC 1 cut(s) 252
Bsu36I CCTNAGG 2 cut(s) 20, 317
BsuRI GGCC 5 cut(s) 143, 501, 519, 567, 594
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 3 cut(s) 58, 327, 412
BtsIMutI CAGTG 2 cut(s) 397, 557
Cac8I GCNNGC 1 cut(s) 100
CciI TCATGA 1 cut(s) 9
CfoI GCGC 2 cut(s) 112, 120
Cfr13I GGNCC 3 cut(s) 4, 223, 565
Cfr9I CCCGGG 1 cut(s) 209
Csp6I GTAC 1 cut(s) 313
CviAII CATG 4 cut(s) 10, 28, 107, 145
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 3 cut(s) 20, 177, 317
DpnI GATC 2 cut(s) 230, 389
DpnII GATC 2 cut(s) 228, 387
EaeI YGGCCR 2 cut(s) 517, 592
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 138, 568
Eco31I GGTCTC 1 cut(s) 540
Eco47I GGWCC 2 cut(s) 4, 223
Eco57I CTGAAG 2 cut(s) 236, 473
Eco81I CCTNAGG 2 cut(s) 20, 317
Eco88I CYCGRG 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 48, 93
EcoT14I CCWWGG 2 cut(s) 138, 568
ErhI CCWWGG 2 cut(s) 138, 568
FaeI CATG 4 cut(s) 13, 31, 110, 148
FaiI YATR 5 cut(s) 11, 29, 108, 126, 146
FatI CATG 4 cut(s) 9, 27, 106, 144
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 5 cut(s) 271, 362, 440, 443, 610
FokI GGATG 3 cut(s) 65, 334, 419
Fsp4HI GCNGC 5 cut(s) 271, 362, 440, 443, 610
FspBI CTAG 1 cut(s) 613
GlaI GCGC 2 cut(s) 111, 119
GluI GCNGC 5 cut(s) 271, 362, 440, 443, 610
GsuI CTGGAG 1 cut(s) 141
HaeIII GGCC 5 cut(s) 143, 501, 519, 567, 594
HapII CCGG 4 cut(s) 130, 210, 365, 446
HhaI GCGC 2 cut(s) 112, 120
Hin1II CATG 4 cut(s) 13, 31, 110, 148
Hin6I GCGC 2 cut(s) 110, 118
HinP1I GCGC 2 cut(s) 110, 118
HincII GTYRAC 2 cut(s) 37, 417
HindII GTYRAC 2 cut(s) 37, 417
HinfI GANTC 2 cut(s) 13, 175
HpaII CCGG 4 cut(s) 130, 210, 365, 446
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 2 cut(s) 37, 417
Hpy188I TCNGA 3 cut(s) 82, 180, 223
Hpy188III TCNNGA 5 cut(s) 10, 242, 349, 404, 578
Hpy8I GTNNAC 2 cut(s) 37, 417
HpyAV CCTTC 4 cut(s) 123, 163, 283, 491
HpyCH4IV ACGT 2 cut(s) 165, 255
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 158, 448, 457, 498, 600, 609
HpyF3I CTNAG 3 cut(s) 20, 177, 317
HpySE526I ACGT 2 cut(s) 165, 255
Hsp92II CATG 4 cut(s) 13, 31, 110, 148
HspAI GCGC 2 cut(s) 110, 118
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 2 cut(s) 228, 387
LmnI GCTCC 1 cut(s) 448
Lsp1109I GCAGC 1 cut(s) 348
LweI GCATC 1 cut(s) 43
MaeI CTAG 1 cut(s) 613
MaeII ACGT 2 cut(s) 165, 255
MalI GATC 2 cut(s) 230, 389
MboI GATC 2 cut(s) 228, 387
MboII GAAGA 5 cut(s) 257, 292, 397, 445, 466
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 2 cut(s) 519, 594
MluNI TGGCCA 2 cut(s) 519, 594
MlyI GAGTC 1 cut(s) 169
MmeI TCCRAC 1 cut(s) 201
Mox20I TGGCCA 2 cut(s) 519, 594
MscI TGGCCA 2 cut(s) 519, 594
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 2 cut(s) 519, 594
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 4 cut(s) 130, 210, 365, 446
MspR9I CCNGG 5 cut(s) 50, 95, 131, 210, 211
Mva1269I GAATGC 2 cut(s) 187, 376
MvaI CCWGG 2 cut(s) 50, 95
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 7 cut(s) 149, 158, 448, 457, 498, 600, 609
NciI CCSGG 3 cut(s) 131, 210, 211
NdeII GATC 2 cut(s) 228, 387
NlaIII CATG 4 cut(s) 13, 31, 110, 148
NlaIV GGNNCC 1 cut(s) 314
NmeAIII GCCGAG 2 cut(s) 315, 421
PagI TCATGA 1 cut(s) 9
PctI GAATGC 2 cut(s) 187, 376
PfeI GAWTC 1 cut(s) 13
PfoI TCCNGGA 2 cut(s) 48, 129
PkrI GCNGC 5 cut(s) 272, 363, 441, 444, 611
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
Psp1406I AACGTT 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 48, 93
PspGI CCWGG 2 cut(s) 48, 93
PspN4I GGNNCC 1 cut(s) 314
PspPI GGNCC 3 cut(s) 4, 223, 565
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 35
SatI GCNGC 5 cut(s) 271, 362, 440, 443, 610
Sau3AI GATC 2 cut(s) 228, 387
Sau96I GGNCC 3 cut(s) 4, 223, 565
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 5 cut(s) 50, 95, 131, 210, 211
SduI GDGCHC 1 cut(s) 210
SfaNI GCATC 1 cut(s) 43
SinI GGWCC 2 cut(s) 4, 223
SmaI CCCGGG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SrfI GCCCGGGC 1 cut(s) 211
SsiI CCGC 5 cut(s) 270, 440, 443, 490, 610
SspMI CTAG 1 cut(s) 613
StyD4I CCNGG 5 cut(s) 48, 93, 129, 208, 209
StyI CCWWGG 2 cut(s) 138, 568
TaiI ACGT 2 cut(s) 168, 258
TaqI TCGA 1 cut(s) 36
TauI GCSGC 4 cut(s) 273, 442, 445, 612
TfiI GAWTC 1 cut(s) 13
TscAI CASTG 2 cut(s) 397, 557
TseI GCWGC 1 cut(s) 361
TspMI CCCGGG 1 cut(s) 209
TspRI CASTG 2 cut(s) 397, 557
VpaK11BI GGWCC 2 cut(s) 4, 223
XmaI CCCGGG 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 36
XspI CTAG 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.