MD08G1068300.v1.1

kDa class II heat shock protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001449
Physical Location & Seq
Reverse (-)
5433838 .. 5434323
486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1068300.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGATGTCAGAATCCAGGGTTTGGATGCCCCGCTCTTCTCCACACTTCAGCACATCATCGACGCCGCCGACGACGCCGAGAAATCCTTCAACGCCCCCACCCGGACCTACTTCCGCGACGCCAAGGCCATGGCCTCCACGCCGGCAGACGTCAAGGAGTACCCGACCTCCTACGTGTTCGTGGTGGACATGCCGGGACTGAAGTCCGGGGACATCAAGGTCCAGGTGGAGGACGACAATGTGCTTCTGATCAGCGGCGAGAGGAAGCGGGAGGAGGAGAAGGAAGGGGCTAAGTACGTGAGGATGGAGCGGAGGGTCGGCAAGTTCATGAGGAAGTTTGTGTTGCCGGAGAATGCTAATGTGGATGCGATTTCTGCTGTTTGCCAGGATGGGGTTTTTGACTGTCACAGTGCAGAAGCTGCCGCCGCCGGAGCCGAAGAAGCCGAAGACAATCGAGGTCAAGATTGCTTGAAACTGTTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

162

Amino Acids

17.84

Weight (kDa)

4.77

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 50 - 134 6.8e-23 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AatII GACGTC 1 cut(s) 153
AccB7I CCANNNNNTGG 1 cut(s) 22
AccBSI CCGCTC 2 cut(s) 34, 310
AccII CGCG 1 cut(s) 117
AciI CCGC 8 cut(s) 32, 66, 115, 255, 268, 310, 423, 426
AcuI CTGAAG 2 cut(s) 32, 221
AcyI GRCGYC 4 cut(s) 63, 75, 120, 150
AfaI GTAC 2 cut(s) 161, 296
AfiI CCNNNNNNNGG 4 cut(s) 22, 102, 229, 391
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 2 cut(s) 91, 472
AjnI CCWGG 3 cut(s) 15, 222, 384
AluBI AGCT 1 cut(s) 419
AluI AGCT 1 cut(s) 419
AlwNI CAGNNNCTG 1 cut(s) 419
AoxI GGCC 2 cut(s) 126, 132
ApeKI GCWGC 1 cut(s) 419
Asp700I GAANNNNTTC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 105, 220
AsuC2I CCSGG 3 cut(s) 103, 195, 208
AvaII GGWCC 2 cut(s) 105, 220
BbsI GAAGAC 1 cut(s) 453
BbvI GCAGC 1 cut(s) 406
BccI CCATC 2 cut(s) 298, 383
BciT130I CCWGG 3 cut(s) 17, 224, 386
BclI TGATCA 1 cut(s) 249
BcnI CCSGG 3 cut(s) 103, 195, 208
BisI GCNGC 5 cut(s) 66, 256, 420, 423, 426
BlsI GCNGC 5 cut(s) 67, 257, 421, 424, 427
Bme1390I CCNGG 6 cut(s) 17, 103, 195, 208, 224, 386
Bme18I GGWCC 2 cut(s) 105, 220
BmgT120I GGNCC 2 cut(s) 105, 220
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 6 cut(s) 17, 103, 195, 208, 224, 386
BmsI GCATC 2 cut(s) 16, 355
BoxI GACNNNNGTC 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 453
BpuMI CCSGG 3 cut(s) 103, 195, 208
BsaAI YACGTR 2 cut(s) 175, 298
BsaHI GRCGYC 4 cut(s) 63, 75, 120, 150
BsaJI CCNNGG 4 cut(s) 16, 123, 129, 207
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 102, 229, 391
Bse118I RCCGGY 1 cut(s) 142
BseBI CCWGG 3 cut(s) 17, 224, 386
BseDI CCNNGG 4 cut(s) 16, 123, 129, 207
BseGI GGATG 5 cut(s) 10, 31, 309, 370, 394
BseLI CCNNNNNNNGG 4 cut(s) 22, 102, 229, 391
BseRI GAGGAG 2 cut(s) 287, 290
BseXI GCAGC 1 cut(s) 406
BsgI GTGCAG 1 cut(s) 432
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 2 cut(s) 128, 134
BsiSI CCGG 6 cut(s) 103, 143, 194, 207, 347, 429
BslFI GGGAC 2 cut(s) 210, 224
BslI CCNNNNNNNGG 4 cut(s) 22, 102, 229, 391
BsmFI GGGAC 2 cut(s) 210, 224
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 2 cut(s) 128, 134
Bsp143I GATC 1 cut(s) 249
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 8 cut(s) 32, 66, 115, 255, 268, 310, 423, 426
BspANI GGCC 2 cut(s) 128, 134
BspFNI CGCG 1 cut(s) 117
BspHI TCATGA 1 cut(s) 327
BspLI GGNNCC 1 cut(s) 433
BspQI GCTCTTC 1 cut(s) 41
BsrBI CCGCTC 2 cut(s) 34, 310
BsrFI RCCGGY 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 4 cut(s) 16, 123, 129, 207
BssMI GATC 1 cut(s) 249
BssNI GRCGYC 4 cut(s) 63, 75, 120, 150
BssT1I CCWWGG 2 cut(s) 123, 129
Bst2UI CCWGG 3 cut(s) 17, 224, 386
Bst4CI ACNGT 3 cut(s) 404, 410, 477
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 4 cut(s) 63, 75, 120, 150
BstAPI GCANNNNNTGC 1 cut(s) 419
BstBAI YACGTR 2 cut(s) 175, 298
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 1 cut(s) 291
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 5 cut(s) 10, 31, 309, 370, 394
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 1 cut(s) 252
BstMBI GATC 1 cut(s) 249
BstMWI GCNNNNNNNGC 6 cut(s) 74, 374, 419, 425, 431, 440
BstNI CCWGG 3 cut(s) 17, 224, 386
BstNSI RCATGY 1 cut(s) 193
BstPAI GACNNNNGTC 1 cut(s) 202
BstSCI CCNGG 6 cut(s) 15, 101, 193, 206, 222, 384
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 1 cut(s) 406
BstV2I GAAGAC 1 cut(s) 453
BstXI CCANNNNNNTGG 1 cut(s) 130
BsuRI GGCC 2 cut(s) 128, 134
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 5 cut(s) 10, 31, 309, 370, 394
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 1 cut(s) 144
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 327
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 2 cut(s) 105, 220
CseI GACGC 3 cut(s) 71, 83, 128
Csp6I GTAC 2 cut(s) 160, 295
CviAII CATG 3 cut(s) 130, 190, 328
CviJI RGCY 6 cut(s) 128, 134, 290, 419, 434, 443
CviKI_1 RGCY 6 cut(s) 128, 134, 290, 419, 434, 443
CviQI GTAC 2 cut(s) 160, 295
DdeI CTNAG 1 cut(s) 291
DpnI GATC 1 cut(s) 251
DpnII GATC 1 cut(s) 249
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 123, 129
Eco47I GGWCC 2 cut(s) 105, 220
Eco57I CTGAAG 2 cut(s) 32, 221
EcoRII CCWGG 3 cut(s) 15, 222, 384
EcoT14I CCWWGG 2 cut(s) 123, 129
ErhI CCWWGG 2 cut(s) 123, 129
FaeI CATG 3 cut(s) 133, 193, 331
FaiI YATR 3 cut(s) 131, 191, 329
FaqI GGGAC 2 cut(s) 210, 224
FatI CATG 3 cut(s) 129, 189, 327
FauI CCCGC 2 cut(s) 39, 261
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 5 cut(s) 66, 256, 420, 423, 426
FokI GGATG 5 cut(s) 17, 38, 316, 377, 401
Fsp4HI GCNGC 5 cut(s) 66, 256, 420, 423, 426
GluI GCNGC 5 cut(s) 66, 256, 420, 423, 426
HaeIII GGCC 2 cut(s) 128, 134
HapII CCGG 6 cut(s) 103, 143, 194, 207, 347, 429
HgaI GACGC 3 cut(s) 71, 83, 128
Hin1I GRCGYC 4 cut(s) 63, 75, 120, 150
Hin1II CATG 3 cut(s) 133, 193, 331
HinfI GANTC 1 cut(s) 12
HpaII CCGG 6 cut(s) 103, 143, 194, 207, 347, 429
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 2 cut(s) 11, 249
Hpy188III TCNNGA 2 cut(s) 328, 461
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 4 cut(s) 65, 74, 77, 122
HpyAV CCTTC 3 cut(s) 97, 274, 278
HpyCH4III ACNGT 3 cut(s) 404, 410, 477
HpyCH4IV ACGT 3 cut(s) 150, 174, 297
HpyCH4V TGCA 1 cut(s) 413
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 374, 419, 425, 431, 440
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 3 cut(s) 150, 174, 297
Hsp92I GRCGYC 4 cut(s) 63, 75, 120, 150
Hsp92II CATG 3 cut(s) 133, 193, 331
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 1 cut(s) 249
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 2 cut(s) 307, 431
Lsp1109I GCAGC 1 cut(s) 406
LweI GCATC 2 cut(s) 16, 355
MaeII ACGT 3 cut(s) 150, 174, 297
MaeIII GTNAC 1 cut(s) 404
MalI GATC 1 cut(s) 251
MbiI CCGCTC 2 cut(s) 34, 310
MboI GATC 1 cut(s) 249
MboII GAAGA 3 cut(s) 28, 449, 458
MroNI GCCGGC 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 255
MspI CCGG 6 cut(s) 103, 143, 194, 207, 347, 429
MspR9I CCNGG 6 cut(s) 17, 103, 195, 208, 224, 386
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 3 cut(s) 17, 224, 386
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 6 cut(s) 74, 374, 419, 425, 431, 440
NaeI GCCGGC 1 cut(s) 144
NciI CCSGG 3 cut(s) 103, 195, 208
NcoI CCATGG 1 cut(s) 129
NdeII GATC 1 cut(s) 249
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 3 cut(s) 133, 193, 331
NlaIV GGNNCC 1 cut(s) 433
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 404
NspI RCATGY 1 cut(s) 193
PagI TCATGA 1 cut(s) 327
PciSI GCTCTTC 1 cut(s) 41
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 69
PctI GAATGC 1 cut(s) 358
PdiI GCCGGC 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 12
PflMI CCANNNNNTGG 1 cut(s) 22
PkrI GCNGC 5 cut(s) 67, 257, 421, 424, 427
Ppu21I YACGTR 2 cut(s) 175, 298
PshAI GACNNNNGTC 1 cut(s) 202
Psp6I CCWGG 3 cut(s) 15, 222, 384
PspGI CCWGG 3 cut(s) 15, 222, 384
PspN4I GGNNCC 1 cut(s) 433
PspPI GGNCC 2 cut(s) 105, 220
PstNI CAGNNNCTG 1 cut(s) 419
RsaI GTAC 2 cut(s) 161, 296
RsaNI GTAC 2 cut(s) 160, 295
SapI GCTCTTC 1 cut(s) 41
SatI GCNGC 5 cut(s) 66, 256, 420, 423, 426
Sau3AI GATC 1 cut(s) 249
Sau96I GGNCC 2 cut(s) 105, 220
ScrFI CCNGG 6 cut(s) 17, 103, 195, 208, 224, 386
SetI ASST 9 cut(s) 110, 153, 170, 177, 222, 228, 300, 421, 460
SfaNI GCATC 2 cut(s) 16, 355
SinI GGWCC 2 cut(s) 105, 220
SsiI CCGC 8 cut(s) 32, 66, 115, 255, 268, 310, 423, 426
StyD4I CCNGG 6 cut(s) 15, 101, 193, 206, 222, 384
StyI CCWWGG 2 cut(s) 123, 129
TaaI ACNGT 3 cut(s) 404, 410, 477
TaiI ACGT 3 cut(s) 153, 177, 300
TaqI TCGA 2 cut(s) 60, 454
TauI GCSGC 4 cut(s) 68, 258, 425, 428
TfiI GAWTC 1 cut(s) 12
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 1 cut(s) 419
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 1 cut(s) 316
TspRI CASTG 1 cut(s) 415
Van91I CCANNNNNTGG 1 cut(s) 22
VpaK11BI GGWCC 2 cut(s) 105, 220
XceI RCATGY 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 86
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.