Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001449

Physical Location & Seq

Genomic Coordinates Reverse (-)

5407860 .. 5408476

Gene Structure 617 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1068000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 465 bp

ATGGATATCAGAATCCAAGGTTTGGATGCCCCACTCTTCTCCACACTTCAGCACATCATCGACGCCGCCGACGACGCCGAGAAGTCCAACGCCCCCACCCGGACCTACGTCCGTGACGCCAAGGCCATGGCCTCCACGCCGGCAGACGTCAAGGAGTACCCGAACTCCTACGTGTTCGTGGTGGACATGCCGGGACTGAAGTCCGGGGACATCAAGGTCCAGGTGGAGGACGACAATGTGCTTCTGATCAGCGGCGAGAGGAAGCGGGAGGAGAAGGAAGGGGCTAAGTACGTGAGGATGGAGCGGAGGGTCGGTAAGTTCATGAGGAAGTTTGTGTTGCCGGAGAACGCGAACGTGGATGCGATTTCTGCTGTTTGCCAGGATGGGGTTTTGACTGTCACGGTGCAGAAGCTGCCGCCGCCGGAGCCGAAGAAGCCGAAGACAATCGAGGTCAAGATTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

HSP20

Protein Analysis

155

Amino Acids

17.1

Weight (kDa)

6.61

Isoelectric Point (pI)

34.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP20	PF00011	49 - 152	8.1e-32	Hsp20/alpha crystallin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001449)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12020 AT5G12030
fragaria_vesca	FvH4_2g31371
malus_domestica	MD00G1181700.v1.1 MD08G1068000.v1.1 MD08G1068200.v1.1 MD08G1068300.v1.1 MD08G1068500.v1.1 MD08G1068700.v1.1 MD08G1068800.v1.1 MD15G1053500.v1.1 MD15G1053600.v1.1 MD15G1053800.v1.1
prunus_persica	Prupe.1G407100_v2.0.a1 Prupe.1G407200_v2.0.a1 Prupe.1G407300_v2.0.a1
pyrus_communis	pycom08g05660 pycom08g05670
rosa_chinensis	RchiOBHm_Chr6g0311451 RchiOBHm_Chr6g0311461 RchiOBHm_Chr6g0311471
rosa_laevigata	RLG00000010403 RLG00000010404 RLG00000010405
rosa_multiflora	Rmu_co8421495.1_g000001 Rmu_sc0006915.1_g000010 Rmu_sc0006915.1_g000011 Rmu_sc0006915.1_g000012 Rmu_sc0013619.1_g000003 Rmu_sc0013619.1_g000004 Rmu_sc0013619.1_g000006 Rmu_sc0015790.1_g000003 Rmu_sc0015790.1_g000004
rosa_roxburghii	Rroxscaffold_7G00157590 Rroxscaffold_7G00157600
rosa_rugosa	Rorug06G0389100 Rorug06G0389100 Rorug06G0389200
rosa_samantha	Rh2AG205100 Rh2BG216900 Rh2DG211400 Rh6AG503500 Rh6AG503700 Rh6BG514200 Rh6BG514300 Rh6CG520000 Rh6CG520100 Rh6DG506100
rosa_wichuraiana	Rw2G016340 Rw6G043850 Rw6G043860 Rw6G043870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	215
AatII	GACGTC	1 cut(s)	150
AccB7I	CCANNNNNTGG	1 cut(s)	22
AccBSI	CCGCTC	1 cut(s)	304
AccII	CGCG	1 cut(s)	350
AciI	CCGC	6 cut(s)	66, 252, 265, 304, 416, 419
AcuI	CTGAAG	2 cut(s)	32, 218
AcyI	GRCGYC	4 cut(s)	63, 75, 117, 147
AfaI	GTAC	2 cut(s)	158, 290
AfiI	CCNNNNNNNGG	4 cut(s)	22, 99, 226, 385
AflIII	ACRYGT	1 cut(s)	171
AjnI	CCWGG	2 cut(s)	219, 378
AluBI	AGCT	1 cut(s)	412
AluI	AGCT	1 cut(s)	412
AlwNI	CAGNNNCTG	1 cut(s)	412
AoxI	GGCC	2 cut(s)	123, 129
ApeKI	GCWGC	1 cut(s)	412
AspS9I	GGNCC	2 cut(s)	102, 217
AsuC2I	CCSGG	3 cut(s)	100, 192, 205
AvaII	GGWCC	2 cut(s)	102, 217
BbsI	GAAGAC	1 cut(s)	446
BbvI	GCAGC	1 cut(s)	399
BccI	CCATC	2 cut(s)	292, 377
BciT130I	CCWGG	2 cut(s)	221, 380
BclI	TGATCA	1 cut(s)	246
BcnI	CCSGG	3 cut(s)	100, 192, 205
BisI	GCNGC	5 cut(s)	66, 253, 413, 416, 419
BlsI	GCNGC	5 cut(s)	67, 254, 414, 417, 420
Bme1390I	CCNGG	5 cut(s)	100, 192, 205, 221, 380
Bme18I	GGWCC	2 cut(s)	102, 217
BmgT120I	GGNCC	2 cut(s)	102, 217
BmiI	GGNNCC	1 cut(s)	426
BmrFI	CCNGG	5 cut(s)	100, 192, 205, 221, 380
BmsI	GCATC	2 cut(s)	16, 349
BoxI	GACNNNNGTC	2 cut(s)	107, 199
BpiI	GAAGAC	1 cut(s)	446
BpuMI	CCSGG	3 cut(s)	100, 192, 205
BsaAI	YACGTR	2 cut(s)	172, 292
BsaHI	GRCGYC	4 cut(s)	63, 75, 117, 147
BsaJI	CCNNGG	4 cut(s)	16, 120, 126, 204
BsaXI	ACNNNNNCTCC	4 cut(s)	149, 179, 298, 328
Bsc4I	CCNNNNNNNGG	4 cut(s)	22, 99, 226, 385
Bse118I	RCCGGY	1 cut(s)	139
BseBI	CCWGG	2 cut(s)	221, 380
BseDI	CCNNGG	4 cut(s)	16, 120, 126, 204
BseGI	GGATG	4 cut(s)	31, 303, 364, 388
BseLI	CCNNNNNNNGG	4 cut(s)	22, 99, 226, 385
BseRI	GAGGAG	1 cut(s)	284
BseXI	GCAGC	1 cut(s)	399
BsgI	GTGCAG	1 cut(s)	425
Bsh1236I	CGCG	1 cut(s)	350
BshFI	GGCC	2 cut(s)	125, 131
BsiSI	CCGG	6 cut(s)	100, 140, 191, 204, 341, 422
BslFI	GGGAC	2 cut(s)	207, 221
BslI	CCNNNNNNNGG	4 cut(s)	22, 99, 226, 385
BsmFI	GGGAC	2 cut(s)	207, 221
BsnI	GGCC	2 cut(s)	125, 131
Bsp143I	GATC	1 cut(s)	246
Bsp19I	CCATGG	1 cut(s)	126
BspACI	CCGC	6 cut(s)	66, 252, 265, 304, 416, 419
BspANI	GGCC	2 cut(s)	125, 131
BspFNI	CGCG	1 cut(s)	350
BspHI	TCATGA	1 cut(s)	321
BspLI	GGNNCC	1 cut(s)	426
BsrBI	CCGCTC	1 cut(s)	304
BsrFI	RCCGGY	1 cut(s)	139
BssAI	RCCGGY	1 cut(s)	139
BssECI	CCNNGG	4 cut(s)	16, 120, 126, 204
BssMI	GATC	1 cut(s)	246
BssNI	GRCGYC	4 cut(s)	63, 75, 117, 147
BssT1I	CCWWGG	3 cut(s)	16, 120, 126
Bst2UI	CCWGG	2 cut(s)	221, 380
Bst4CI	ACNGT	2 cut(s)	397, 403
Bst6I	CTCTTC	1 cut(s)	41
BstACI	GRCGYC	4 cut(s)	63, 75, 117, 147
BstAPI	GCANNNNNTGC	1 cut(s)	412
BstBAI	YACGTR	2 cut(s)	172, 292
BstC8I	GCNNGC	1 cut(s)	141
BstDEI	CTNAG	1 cut(s)	285
BstDSI	CCRYGG	1 cut(s)	126
BstF5I	GGATG	4 cut(s)	31, 303, 364, 388
BstFNI	CGCG	1 cut(s)	350
BstKTI	GATC	1 cut(s)	249
BstMBI	GATC	1 cut(s)	246
BstMWI	GCNNNNNNNGC	6 cut(s)	74, 368, 412, 418, 424, 433
BstNI	CCWGG	2 cut(s)	221, 380
BstNSI	RCATGY	1 cut(s)	190
BstPAI	GACNNNNGTC	2 cut(s)	107, 199
BstSCI	CCNGG	5 cut(s)	98, 190, 203, 219, 378
BstUI	CGCG	1 cut(s)	350
BstV1I	GCAGC	1 cut(s)	399
BstV2I	GAAGAC	1 cut(s)	446
BstXI	CCANNNNNNTGG	1 cut(s)	127
BsuRI	GGCC	2 cut(s)	125, 131
BtgI	CCRYGG	1 cut(s)	126
BtsCI	GGATG	4 cut(s)	31, 303, 364, 388
Cac8I	GCNNGC	1 cut(s)	141
CaiI	CAGNNNCTG	1 cut(s)	412
CciI	TCATGA	1 cut(s)	321
Cfr10I	RCCGGY	1 cut(s)	139
Cfr13I	GGNCC	2 cut(s)	102, 217
CseI	GACGC	3 cut(s)	71, 83, 125
Csp6I	GTAC	2 cut(s)	157, 289
CviAII	CATG	3 cut(s)	127, 187, 322
CviJI	RGCY	6 cut(s)	125, 131, 284, 412, 427, 436
CviKI_1	RGCY	6 cut(s)	125, 131, 284, 412, 427, 436
CviQI	GTAC	2 cut(s)	157, 289
DdeI	CTNAG	1 cut(s)	285
DpnI	GATC	1 cut(s)	248
DpnII	GATC	1 cut(s)	246
DrdI	GACNNNNNNGTC	1 cut(s)	215
DseDI	GACNNNNNNGTC	1 cut(s)	215
Eam1104I	CTCTTC	1 cut(s)	41
EarI	CTCTTC	1 cut(s)	41
Eco130I	CCWWGG	3 cut(s)	16, 120, 126
Eco32I	GATATC	1 cut(s)	7
Eco47I	GGWCC	2 cut(s)	102, 217
Eco57I	CTGAAG	2 cut(s)	32, 218
EcoRII	CCWGG	2 cut(s)	219, 378
EcoRV	GATATC	1 cut(s)	7
EcoT14I	CCWWGG	3 cut(s)	16, 120, 126
ErhI	CCWWGG	3 cut(s)	16, 120, 126
FaeI	CATG	3 cut(s)	130, 190, 325
FaiI	YATR	3 cut(s)	128, 188, 323
FaqI	GGGAC	2 cut(s)	207, 221
FatI	CATG	3 cut(s)	126, 186, 321
FauI	CCCGC	1 cut(s)	258
FbaI	TGATCA	1 cut(s)	246
Fnu4HI	GCNGC	5 cut(s)	66, 253, 413, 416, 419
FokI	GGATG	4 cut(s)	38, 310, 371, 395
Fsp4HI	GCNGC	5 cut(s)	66, 253, 413, 416, 419
GluI	GCNGC	5 cut(s)	66, 253, 413, 416, 419
HaeIII	GGCC	2 cut(s)	125, 131
HapII	CCGG	6 cut(s)	100, 140, 191, 204, 341, 422
HgaI	GACGC	3 cut(s)	71, 83, 125
Hin1I	GRCGYC	4 cut(s)	63, 75, 117, 147
Hin1II	CATG	3 cut(s)	130, 190, 325
HinfI	GANTC	1 cut(s)	12
HpaII	CCGG	6 cut(s)	100, 140, 191, 204, 341, 422
Hpy166II	GTNNAC	1 cut(s)	184
Hpy188I	TCNGA	2 cut(s)	11, 246
Hpy188III	TCNNGA	2 cut(s)	322, 454
Hpy8I	GTNNAC	1 cut(s)	184
Hpy99I	CGWCG	3 cut(s)	65, 74, 77
HpyAV	CCTTC	2 cut(s)	268, 272
HpyCH4III	ACNGT	2 cut(s)	397, 403
HpyCH4IV	ACGT	5 cut(s)	108, 147, 171, 291, 354
HpyCH4V	TGCA	1 cut(s)	406
HpyF10VI	GCNNNNNNNGC	6 cut(s)	74, 368, 412, 418, 424, 433
HpyF3I	CTNAG	1 cut(s)	285
HpySE526I	ACGT	5 cut(s)	108, 147, 171, 291, 354
Hsp92I	GRCGYC	4 cut(s)	63, 75, 117, 147
Hsp92II	CATG	3 cut(s)	130, 190, 325
KroI	GCCGGC	1 cut(s)	139
KroNI	GCCGGC	1 cut(s)	141
Ksp22I	TGATCA	1 cut(s)	246
Kzo9I	GATC	1 cut(s)	246
LmnI	GCTCC	2 cut(s)	301, 424
Lsp1109I	GCAGC	1 cut(s)	399
LweI	GCATC	2 cut(s)	16, 349
MaeII	ACGT	5 cut(s)	108, 147, 171, 291, 354
MaeIII	GTNAC	2 cut(s)	113, 397
MalI	GATC	1 cut(s)	248
MbiI	CCGCTC	1 cut(s)	304
MboI	GATC	1 cut(s)	246
MboII	GAAGA	3 cut(s)	28, 442, 451
MmeI	TCCRAC	1 cut(s)	111
MnlI	CCTC	8 cut(s)	142, 220, 252, 262, 288, 300, 318, 442
MroNI	GCCGGC	1 cut(s)	139
MspA1I	CMGCKG	1 cut(s)	252
MspI	CCGG	6 cut(s)	100, 140, 191, 204, 341, 422
MspR9I	CCNGG	5 cut(s)	100, 192, 205, 221, 380
MvaI	CCWGG	2 cut(s)	221, 380
MvnI	CGCG	1 cut(s)	350
MwoI	GCNNNNNNNGC	6 cut(s)	74, 368, 412, 418, 424, 433
NaeI	GCCGGC	1 cut(s)	141
NciI	CCSGG	3 cut(s)	100, 192, 205
NcoI	CCATGG	1 cut(s)	126
NdeII	GATC	1 cut(s)	246
NgoMIV	GCCGGC	1 cut(s)	139
NlaIII	CATG	3 cut(s)	130, 190, 325
NlaIV	GGNNCC	1 cut(s)	426
NmeAIII	GCCGAG	1 cut(s)	103
NmuCI	GTSAC	2 cut(s)	113, 397
NspI	RCATGY	1 cut(s)	190
PagI	TCATGA	1 cut(s)	321
PcsI	WCGNNNNNNNCGW	2 cut(s)	66, 69
PdiI	GCCGGC	1 cut(s)	141
PfeI	GAWTC	1 cut(s)	12
PflMI	CCANNNNNTGG	1 cut(s)	22
PkrI	GCNGC	5 cut(s)	67, 254, 414, 417, 420
Ppu21I	YACGTR	2 cut(s)	172, 292
PshAI	GACNNNNGTC	2 cut(s)	107, 199
Psp6I	CCWGG	2 cut(s)	219, 378
PspGI	CCWGG	2 cut(s)	219, 378
PspN4I	GGNNCC	1 cut(s)	426
PspPI	GGNCC	2 cut(s)	102, 217
PstNI	CAGNNNCTG	1 cut(s)	412
RsaI	GTAC	2 cut(s)	158, 290
RsaNI	GTAC	2 cut(s)	157, 289
SatI	GCNGC	5 cut(s)	66, 253, 413, 416, 419
Sau3AI	GATC	1 cut(s)	246
Sau96I	GGNCC	2 cut(s)	102, 217
ScrFI	CCNGG	5 cut(s)	100, 192, 205, 221, 380
SfaNI	GCATC	2 cut(s)	16, 349
SinI	GGWCC	2 cut(s)	102, 217
SsiI	CCGC	6 cut(s)	66, 252, 265, 304, 416, 419
StyD4I	CCNGG	5 cut(s)	98, 190, 203, 219, 378
StyI	CCWWGG	3 cut(s)	16, 120, 126
TaaI	ACNGT	2 cut(s)	397, 403
TaiI	ACGT	5 cut(s)	111, 150, 174, 294, 357
TaqI	TCGA	2 cut(s)	60, 447
TauI	GCSGC	4 cut(s)	68, 255, 418, 421
TfiI	GAWTC	1 cut(s)	12
TseFI	GTSAC	2 cut(s)	113, 397
TseI	GCWGC	1 cut(s)	412
Tsp45I	GTSAC	2 cut(s)	113, 397
TspDTI	ATGAA	1 cut(s)	310
TspGWI	ACGGA	1 cut(s)	101
Van91I	CCANNNNNTGG	1 cut(s)	22
VpaK11BI	GGWCC	2 cut(s)	102, 217
XceI	RCATGY	1 cut(s)	190
ZraI	GACGTC	1 cut(s)	148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.