pycom08g05660

kDa class II heat shock protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001449
Physical Location & Seq
Reverse (-)
4290464 .. 4292615
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05660.1

Sequence Viewer

Length: 1284 bp
ATGGAGGTTATGAGCTTGCGGAACTTGGGTTTCGACCCCAACCTGCTGGAGACCCTGCACGACCTGCTCGACATCTCCGACGAGCAGAACCAACAAAGCTACCAGGCGCCCTCTCGCCGGTACGTCCGCGAGGCGAAGGCCATGGCCGCCACACCCGCAGATGTAAAGGAGACTAAAGATGCCTACGTTTTCGTGGTGGATGTTCCAGGGCTGAGGCCGGATATGATTAATGTGAAGATTGAGGACGACAACGTGCTGTTGGTGAGCGGGGAGAGGAAGAGAGAGAAGGAGAGGGATCAGGGGATCAAGTACTTGAGGATGGAGAGGAGGCTCGGGAAGTATCTCAAGAAGTTTGTGCTGCCGGACAACGCCGATATTGAGAGGATTTCGGCCGAGTGTCAGGATGGGGTGCTGACCGTGACAGTTGCGAAGAAGCCGCTGCCAAAGCAGAAGAAGCCCAAGACCATTCAGATCCAGATAAGCAGCAACCAAGGTGGCGGTCAGGGAGGCAAAGGTGGTCAAGGCTGGGAAGAAGGTGGAGAAGACGAACGTGGCAAGCAGAGCGGTGGCCAAGGTTGGGAACATGGCAGGCAAGGTGGTGGCCAGGGAGGCGAAGGTGGTCAAGGTTGGGAAGAAGGTGGCCAAGAGGAACGTGGTAGGCAAGGCCGTGGGCAAGGTGGCCAAGGCGGTGAGCAAGGTGGGGAACATGACAGGCAAGGTGGGCAGAGCGGTGGGAAGCGTGGTGCCGGTGGGTATGAAGGGCAAGTTGAAGGGCGTGAGAAAGCGAAAATTCCATCCACAAAGAAGCGAAGAATGGACATCAGAATCCCCGGTTTGGAATCCCCGCTCTTGTCCACGCTTCAGCACATGATGGACGCGGCCGACGACTCTGAGAAGTCCTTCAACGCCCCCACCCGGACCTACGTCCGCGACGCCAAGGCCATGGCCTCCACGCCGGCAGACGTCAAGGAGTACCCGACCTCGTACGTGTTCGTGGTGGACATGCCGGGACTGAAGTCCGGGGACATCAAGGTCCAGGTGGAGGACGACAATGTGCTTCTGATCAGCGGCGAGAGGAAGCGGGAGGAGGAGAAGGAAGGGGCTAAGTACGTGAGGATGGAGCGGAGGGTCGGCAAGTTCATGAGGAAGTTTGTGTTACCGGAGAATGCGAACGTGGACGCGATTTCTGCTGTTTGCCAGGATGGGGTTTTGACTGTCACGGTGCAGAAGCTGCCGCCGCCGGAGCCGAAGAAGCCGAAGACAATCGAGGTCAAGATTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

428

Amino Acids

46.96

Weight (kDa)

7.65

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 54 - 159 1.6e-27 Hsp20/alpha crystallin family
HSP20 PF00011 321 - 425 3.1e-30 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1031
AatII GACGTC 1 cut(s) 966
Acc36I ACCTGC 2 cut(s) 51, 72
AccB1I GGYRCC 2 cut(s) 106, 743
AccBSI CCGCTC 5 cut(s) 267, 564, 729, 847, 1123
AccII CGCG 4 cut(s) 129, 878, 930, 1181
AclWI GGATC 3 cut(s) 303, 311, 466
AcoI YGGCCR 7 cut(s) 144, 390, 568, 601, 640, 679, 879
AcsI RAATTY 1 cut(s) 789
AcuI CTGAAG 2 cut(s) 845, 1034
AcyI GRCGYC 3 cut(s) 107, 933, 963
AfaI GTAC 5 cut(s) 122, 311, 974, 986, 1109
AfiI CCNNNNNNNGG 6 cut(s) 117, 577, 835, 915, 1042, 1204
AflIII ACRYGT 1 cut(s) 987
AgsI TTSAA 2 cut(s) 770, 904
AjnI CCWGG 5 cut(s) 102, 205, 603, 1035, 1197
AluBI AGCT 3 cut(s) 15, 99, 1231
AluI AGCT 3 cut(s) 15, 99, 1231
Alw26I GTCTC 2 cut(s) 44, 164
AlwI GGATC 3 cut(s) 303, 311, 466
AlwNI CAGNNNCTG 1 cut(s) 1231
Ama87I CYCGRG 1 cut(s) 332
ApeKI GCWGC 4 cut(s) 358, 439, 483, 1231
ApoI RAATTY 1 cut(s) 789
AseI ATTAAT 1 cut(s) 228
Asp700I GAANNNNTTC 1 cut(s) 899
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 2 cut(s) 918, 1033
AsuC2I CCSGG 4 cut(s) 831, 916, 1008, 1021
AsuHPI GGTGA 2 cut(s) 274, 701
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 2 cut(s) 918, 1033
BalI TGGCCA 4 cut(s) 570, 603, 642, 681
BanI GGYRCC 2 cut(s) 106, 743
BbsI GAAGAC 2 cut(s) 549, 1265
BbvCI CCTCAGC 1 cut(s) 212
BbvI GCAGC 4 cut(s) 345, 426, 495, 1218
BccI CCATC 6 cut(s) 313, 398, 802, 865, 1111, 1196
BceAI ACGGC 1 cut(s) 651
BciT130I CCWGG 5 cut(s) 104, 207, 605, 1037, 1199
BclI TGATCA 1 cut(s) 1062
BcnI CCSGG 4 cut(s) 831, 916, 1008, 1021
BcoDI GTCTC 2 cut(s) 44, 164
BfoI RGCGCY 1 cut(s) 110
BfuAI ACCTGC 2 cut(s) 51, 72
BglI GCCNNNNNGGC 1 cut(s) 609
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 9 cut(s) 104, 207, 605, 831, 916, 1008, 1021, 1037, 1199
Bme18I GGWCC 2 cut(s) 918, 1033
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 2 cut(s) 918, 1033
BmiI GGNNCC 3 cut(s) 108, 745, 1245
BmrFI CCNGG 9 cut(s) 104, 207, 605, 831, 916, 1008, 1021, 1037, 1199
BmsI GCATC 1 cut(s) 169
BoxI GACNNNNGTC 2 cut(s) 923, 1015
BpiI GAAGAC 2 cut(s) 549, 1265
BpmI CTGGAG 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 212
BpuEI CTTGAG 2 cut(s) 329, 334
BpuMI CCSGG 4 cut(s) 831, 916, 1008, 1021
BsaAI YACGTR 2 cut(s) 988, 1111
BsaHI GRCGYC 3 cut(s) 107, 933, 963
BsaI GGTCTC 1 cut(s) 44
BsaWI WCCGGW 1 cut(s) 1159
Bsc4I CCNNNNNNNGG 6 cut(s) 117, 577, 835, 915, 1042, 1204
Bse118I RCCGGY 3 cut(s) 117, 746, 955
BseBI CCWGG 5 cut(s) 104, 207, 605, 1037, 1199
BseGI GGATG 6 cut(s) 205, 324, 409, 794, 1122, 1207
BseLI CCNNNNNNNGG 6 cut(s) 117, 577, 835, 915, 1042, 1204
BseMII CTCAG 2 cut(s) 203, 882
BseRI GAGGAG 3 cut(s) 340, 1100, 1103
BseX3I CGGCCG 2 cut(s) 390, 879
BseXI GCAGC 4 cut(s) 345, 426, 495, 1218
BseYI CCCAGC 1 cut(s) 525
BsgI GTGCAG 2 cut(s) 41, 1244
Bsh1236I CGCG 4 cut(s) 129, 878, 930, 1181
Bsh1285I CGRYCG 2 cut(s) 393, 882
BshNI GGYRCC 2 cut(s) 106, 743
BsiEI CGRYCG 2 cut(s) 393, 882
BsiHKCI CYCGRG 1 cut(s) 332
BsiWI CGTACG 1 cut(s) 984
BslFI GGGAC 2 cut(s) 1023, 1037
BslI CCNNNNNNNGG 6 cut(s) 117, 577, 835, 915, 1042, 1204
BsmAI GTCTC 2 cut(s) 44, 164
BsmFI GGGAC 2 cut(s) 1023, 1037
BsmI GAATGC 1 cut(s) 1171
Bso31I GGTCTC 1 cut(s) 44
BsoBI CYCGRG 1 cut(s) 332
Bsp143I GATC 4 cut(s) 295, 303, 471, 1062
Bsp19I CCATGG 2 cut(s) 141, 942
BspCNI CTCAG 2 cut(s) 204, 883
BspFNI CGCG 4 cut(s) 129, 878, 930, 1181
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 3 cut(s) 108, 745, 1245
BspMI ACCTGC 2 cut(s) 51, 72
BspPI GGATC 3 cut(s) 303, 311, 466
BspT107I GGYRCC 2 cut(s) 106, 743
BspTNI GGTCTC 1 cut(s) 44
BsrBI CCGCTC 5 cut(s) 267, 564, 729, 847, 1123
BsrFI RCCGGY 3 cut(s) 117, 746, 955
BssAI RCCGGY 3 cut(s) 117, 746, 955
BssMI GATC 4 cut(s) 295, 303, 471, 1062
BssNI GRCGYC 3 cut(s) 107, 933, 963
BssT1I CCWWGG 6 cut(s) 141, 490, 571, 682, 936, 942
Bst2UI CCWGG 5 cut(s) 104, 207, 605, 1037, 1199
Bst4CI ACNGT 4 cut(s) 418, 424, 1216, 1222
Bst6I CTCTTC 1 cut(s) 272
BstACI GRCGYC 3 cut(s) 107, 933, 963
BstAPI GCANNNNNTGC 2 cut(s) 64, 1231
BstBAI YACGTR 2 cut(s) 988, 1111
BstC8I GCNNGC 4 cut(s) 17, 557, 590, 957
BstDEI CTNAG 3 cut(s) 212, 891, 1104
BstDSI CCRYGG 3 cut(s) 141, 667, 942
BstF5I GGATG 6 cut(s) 205, 324, 409, 794, 1122, 1207
BstFNI CGCG 4 cut(s) 129, 878, 930, 1181
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 4 cut(s) 298, 306, 474, 1065
BstMAI GTCTC 2 cut(s) 44, 164
BstMBI GATC 4 cut(s) 295, 303, 471, 1062
BstMCI CGRYCG 2 cut(s) 393, 882
BstNI CCWGG 5 cut(s) 104, 207, 605, 1037, 1199
BstNSI RCATGY 1 cut(s) 1006
BstPAI GACNNNNGTC 2 cut(s) 923, 1015
BstSCI CCNGG 9 cut(s) 102, 205, 603, 829, 914, 1006, 1019, 1035, 1197
BstUI CGCG 4 cut(s) 129, 878, 930, 1181
BstV1I GCAGC 4 cut(s) 345, 426, 495, 1218
BstV2I GAAGAC 2 cut(s) 549, 1265
BstX2I RGATCY 1 cut(s) 471
BstXI CCANNNNNNTGG 2 cut(s) 46, 943
BstYI RGATCY 1 cut(s) 471
BstZI CGGCCG 2 cut(s) 390, 879
BtgI CCRYGG 3 cut(s) 141, 667, 942
BtsCI GGATG 6 cut(s) 205, 324, 409, 794, 1122, 1207
BveI ACCTGC 2 cut(s) 51, 72
Cac8I GCNNGC 4 cut(s) 17, 557, 590, 957
CaiI CAGNNNCTG 1 cut(s) 1231
CciI TCATGA 1 cut(s) 1140
CfoI GCGC 1 cut(s) 109
Cfr10I RCCGGY 3 cut(s) 117, 746, 955
Cfr13I GGNCC 2 cut(s) 918, 1033
CseI GACGC 3 cut(s) 884, 941, 1187
Csp6I GTAC 5 cut(s) 121, 310, 973, 985, 1108
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviAII CATG 7 cut(s) 142, 584, 707, 868, 943, 1003, 1141
CviQI GTAC 5 cut(s) 121, 310, 973, 985, 1108
DdeI CTNAG 3 cut(s) 212, 891, 1104
DinI GGCGCC 1 cut(s) 108
DpnI GATC 4 cut(s) 297, 305, 473, 1064
DpnII GATC 4 cut(s) 295, 303, 471, 1062
DrdI GACNNNNNNGTC 1 cut(s) 1031
DseDI GACNNNNNNGTC 1 cut(s) 1031
EaeI YGGCCR 7 cut(s) 144, 390, 568, 601, 640, 679, 879
EagI CGGCCG 2 cut(s) 390, 879
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
EclXI CGGCCG 2 cut(s) 390, 879
Eco130I CCWWGG 6 cut(s) 141, 490, 571, 682, 936, 942
Eco31I GGTCTC 1 cut(s) 44
Eco47I GGWCC 2 cut(s) 918, 1033
Eco52I CGGCCG 2 cut(s) 390, 879
Eco57I CTGAAG 2 cut(s) 845, 1034
Eco88I CYCGRG 1 cut(s) 332
EcoRII CCWGG 5 cut(s) 102, 205, 603, 1035, 1197
EcoT14I CCWWGG 6 cut(s) 141, 490, 571, 682, 936, 942
EgeI GGCGCC 1 cut(s) 108
EheI GGCGCC 1 cut(s) 108
ErhI CCWWGG 6 cut(s) 141, 490, 571, 682, 936, 942
FaeI CATG 7 cut(s) 145, 587, 710, 871, 946, 1006, 1144
FaqI GGGAC 2 cut(s) 1023, 1037
FatI CATG 7 cut(s) 141, 583, 706, 867, 942, 1002, 1140
FauI CCCGC 4 cut(s) 163, 260, 852, 1074
FbaI TGATCA 1 cut(s) 1062
FokI GGATG 6 cut(s) 212, 331, 416, 781, 1129, 1214
GlaI GCGC 1 cut(s) 108
GsaI CCCAGC 1 cut(s) 529
GsuI CTGGAG 1 cut(s) 68
HaeII RGCGCY 1 cut(s) 110
HgaI GACGC 3 cut(s) 884, 941, 1187
HhaI GCGC 1 cut(s) 109
Hin1I GRCGYC 3 cut(s) 107, 933, 963
Hin1II CATG 7 cut(s) 145, 587, 710, 871, 946, 1006, 1144
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HinfI GANTC 3 cut(s) 825, 839, 887
HphI GGTGA 2 cut(s) 274, 701
Hpy166II GTNNAC 3 cut(s) 855, 1000, 1177
Hpy188I TCNGA 5 cut(s) 79, 471, 824, 892, 1062
Hpy188III TCNNGA 6 cut(s) 334, 346, 401, 475, 1141, 1273
Hpy8I GTNNAC 3 cut(s) 855, 1000, 1177
Hpy99I CGWCG 3 cut(s) 83, 887, 935
HpyCH4III ACNGT 4 cut(s) 418, 424, 1216, 1222
HpyCH4V TGCA 2 cut(s) 58, 1225
HpyF3I CTNAG 3 cut(s) 212, 891, 1104
Hsp92I GRCGYC 3 cut(s) 107, 933, 963
Hsp92II CATG 7 cut(s) 145, 587, 710, 871, 946, 1006, 1144
HspAI GCGC 1 cut(s) 107
KasI GGCGCC 1 cut(s) 106
KroI GCCGGC 1 cut(s) 955
KroNI GCCGGC 1 cut(s) 957
Ksp22I TGATCA 1 cut(s) 1062
Kzo9I GATC 4 cut(s) 295, 303, 471, 1062
LmnI GCTCC 2 cut(s) 1120, 1243
Lsp1109I GCAGC 4 cut(s) 345, 426, 495, 1218
LweI GCATC 1 cut(s) 169
MaeIII GTNAC 3 cut(s) 418, 1155, 1216
MalI GATC 4 cut(s) 297, 305, 473, 1064
MbiI CCGCTC 5 cut(s) 267, 564, 729, 847, 1123
MboI GATC 4 cut(s) 295, 303, 471, 1062
MflI RGATCY 1 cut(s) 471
MlsI TGGCCA 4 cut(s) 570, 603, 642, 681
MluCI AATT 1 cut(s) 789
MluNI TGGCCA 4 cut(s) 570, 603, 642, 681
Mly113I GGCGCC 1 cut(s) 107
MlyI GAGTC 1 cut(s) 881
MmeI TCCRAC 1 cut(s) 102
Mox20I TGGCCA 4 cut(s) 570, 603, 642, 681
MroNI GCCGGC 1 cut(s) 955
MroXI GAANNNNTTC 1 cut(s) 899
MscI TGGCCA 4 cut(s) 570, 603, 642, 681
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 4 cut(s) 570, 603, 642, 681
MspA1I CMGCKG 2 cut(s) 439, 1068
MspR9I CCNGG 9 cut(s) 104, 207, 605, 831, 916, 1008, 1021, 1037, 1199
Mva1269I GAATGC 1 cut(s) 1171
MvaI CCWGG 5 cut(s) 104, 207, 605, 1037, 1199
MvnI CGCG 4 cut(s) 129, 878, 930, 1181
NaeI GCCGGC 1 cut(s) 957
NarI GGCGCC 1 cut(s) 107
NciI CCSGG 4 cut(s) 831, 916, 1008, 1021
NcoI CCATGG 2 cut(s) 141, 942
NdeII GATC 4 cut(s) 295, 303, 471, 1062
NgoMIV GCCGGC 1 cut(s) 955
NlaIII CATG 7 cut(s) 145, 587, 710, 871, 946, 1006, 1144
NlaIV GGNNCC 3 cut(s) 108, 745, 1245
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 2 cut(s) 418, 1216
NspI RCATGY 1 cut(s) 1006
PagI TCATGA 1 cut(s) 1140
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 75, 882
PctI GAATGC 1 cut(s) 1171
PdiI GCCGGC 1 cut(s) 957
PdmI GAANNNNTTC 1 cut(s) 899
PfeI GAWTC 2 cut(s) 825, 839
Pfl23II CGTACG 1 cut(s) 984
PleI GAGTC 1 cut(s) 881
PluTI GGCGCC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 881
Ppu21I YACGTR 2 cut(s) 988, 1111
PshAI GACNNNNGTC 2 cut(s) 923, 1015
PshBI ATTAAT 1 cut(s) 228
Psp6I CCWGG 5 cut(s) 102, 205, 603, 1035, 1197
PspFI CCCAGC 1 cut(s) 525
PspGI CCWGG 5 cut(s) 102, 205, 603, 1035, 1197
PspLI CGTACG 1 cut(s) 984
PspN4I GGNNCC 3 cut(s) 108, 745, 1245
PspPI GGNCC 2 cut(s) 918, 1033
PstNI CAGNNNCTG 1 cut(s) 1231
PsuI RGATCY 1 cut(s) 471
RsaI GTAC 5 cut(s) 122, 311, 974, 986, 1109
RsaNI GTAC 5 cut(s) 121, 310, 973, 985, 1108
SaqAI TTAA 1 cut(s) 228
Sau3AI GATC 4 cut(s) 295, 303, 471, 1062
Sau96I GGNCC 2 cut(s) 918, 1033
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 9 cut(s) 104, 207, 605, 831, 916, 1008, 1021, 1037, 1199
SfaNI GCATC 1 cut(s) 169
SfoI GGCGCC 1 cut(s) 108
SinI GGWCC 2 cut(s) 918, 1033
SmlI CTYRAG 2 cut(s) 313, 344
SmoI CTYRAG 2 cut(s) 313, 344
Sse9I AATT 1 cut(s) 789
SspDI GGCGCC 1 cut(s) 106
StyD4I CCNGG 9 cut(s) 102, 205, 603, 829, 914, 1006, 1019, 1035, 1197
StyI CCWWGG 6 cut(s) 141, 490, 571, 682, 936, 942
TaaI ACNGT 4 cut(s) 418, 424, 1216, 1222
TaqI TCGA 3 cut(s) 33, 69, 1266
TasI AATT 1 cut(s) 789
TatI WGTACW 1 cut(s) 309
TauI GCSGC 6 cut(s) 149, 439, 881, 1071, 1237, 1240
TfiI GAWTC 2 cut(s) 825, 839
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseFI GTSAC 2 cut(s) 418, 1216
TseI GCWGC 4 cut(s) 358, 439, 483, 1231
Tsp45I GTSAC 2 cut(s) 418, 1216
TspDTI ATGAA 2 cut(s) 771, 1129
VpaK11BI GGWCC 2 cut(s) 918, 1033
VspI ATTAAT 1 cut(s) 228
XapI RAATTY 1 cut(s) 789
XceI RCATGY 1 cut(s) 1006
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XmnI GAANNNNTTC 1 cut(s) 899
ZraI GACGTC 1 cut(s) 964
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.