MD08G1068500.v1.1

kDa class II heat shock protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001449
Physical Location & Seq
Reverse (-)
5449001 .. 5449663
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1068500.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGAAGTGATGAGCCTGAGGAACTTGGGTTTCGATCCCAACCTGCTGGAGACCCTGCACGACCTGCTCGACATCTCCGACGAGCAGAACCAACAAAGCTACCAGGCCCCCTCTCGCCGGTACGTCCGGGAGGCGAAGGCCATGGCTGCCACACCCGCAGATGTGAAGGAGACTAAAGATGCGTACGTTTTCGTGGTGGATGTTCCAGGGCTGAGGCCGGATATGATTAATGTGAAGATTGAGGAGGACAACGTGCTGGTGGTGAGCGGGGAGAGGAAGAAAGAGAAGGAGAGGGATCAGGGGATCAAGTACTTGAGGATGGAGAGGAGGCTCGGGAAGTATCTCAAGAAGTTTGTGCTGCCGGACAACGCCGATATTGAGAGGATTTCGGCCGAGTGTCAGGATGGGGTGCTGACTGTGACAGTTGCGAAGAAGCCGCCGCCAGAGCAGAAGAAGCCCAAGACTATTCAGATCCAGATAAGCAGCAACCAAGGCGGCGGCCAGGGAGGCAAAGGTGGTCAAGGCTGGGAAGAAGGTGGCCAAGAGGAACGTGGTAGGCAAGGCGGTGAGCAAGGTGGGGAACATGGCAGGCAAGGTGGGCAGAGCGGTGGGGAGCGTGGTGCCGGTGGGTATGAAGGACAAGTTGAAGGGCGTGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

221

Amino Acids

24.25

Weight (kDa)

5.66

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 54 - 159 6.5e-29 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 51, 72
AccB1I GGYRCC 1 cut(s) 620
AccBSI CCGCTC 2 cut(s) 267, 606
AciI CCGC 8 cut(s) 156, 267, 437, 440, 495, 498, 564, 606
AclWI GGATC 4 cut(s) 29, 303, 311, 466
AcoI YGGCCR 3 cut(s) 390, 499, 538
AfaI GTAC 3 cut(s) 122, 185, 311
AfiI CCNNNNNNNGG 1 cut(s) 117
AgsI TTSAA 1 cut(s) 647
AjnI CCWGG 3 cut(s) 102, 205, 501
AluBI AGCT 1 cut(s) 99
AluI AGCT 1 cut(s) 99
Alw26I GTCTC 2 cut(s) 44, 164
AlwI GGATC 4 cut(s) 29, 303, 311, 466
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 6 cut(s) 105, 138, 215, 390, 499, 538
ApeKI GCWGC 3 cut(s) 146, 358, 483
AseI ATTAAT 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 2 cut(s) 274, 578
AvaI CYCGRG 1 cut(s) 332
AxyI CCTNAGG 1 cut(s) 17
BalI TGGCCA 1 cut(s) 540
BanI GGYRCC 1 cut(s) 620
BbvCI CCTCAGC 1 cut(s) 212
BbvI GCAGC 3 cut(s) 133, 345, 495
BccI CCATC 2 cut(s) 313, 398
BciT130I CCWGG 3 cut(s) 104, 207, 503
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 44, 164
BfuAI ACCTGC 2 cut(s) 51, 72
BglI GCCNNNNNGGC 1 cut(s) 507
BisI GCNGC 7 cut(s) 147, 359, 437, 440, 484, 496, 499
BlsI GCNGC 7 cut(s) 148, 360, 438, 441, 485, 497, 500
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 4 cut(s) 104, 128, 207, 503
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 2 cut(s) 108, 622
BmrFI CCNGG 4 cut(s) 104, 128, 207, 503
BmsI GCATC 1 cut(s) 169
BpmI CTGGAG 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 212
BpuEI CTTGAG 2 cut(s) 329, 334
BpuMI CCSGG 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 4 cut(s) 141, 206, 490, 502
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse118I RCCGGY 2 cut(s) 117, 623
Bse21I CCTNAGG 1 cut(s) 17
BseBI CCWGG 3 cut(s) 104, 207, 503
BseDI CCNNGG 4 cut(s) 141, 206, 490, 502
BseGI GGATG 3 cut(s) 205, 324, 409
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMII CTCAG 2 cut(s) 8, 203
BseRI GAGGAG 2 cut(s) 257, 340
BseX3I CGGCCG 1 cut(s) 390
BseXI GCAGC 3 cut(s) 133, 345, 495
BseYI CCCAGC 1 cut(s) 525
BsgI GTGCAG 1 cut(s) 41
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 6 cut(s) 107, 140, 217, 392, 501, 540
BshNI GGYRCC 1 cut(s) 620
BsiEI CGRYCG 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 5 cut(s) 118, 127, 218, 362, 624
BsiWI CGTACG 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 117
BsmAI GTCTC 2 cut(s) 44, 164
BsnI GGCC 6 cut(s) 107, 140, 217, 392, 501, 540
Bso31I GGTCTC 1 cut(s) 44
BsoBI CYCGRG 1 cut(s) 332
Bsp143I GATC 4 cut(s) 34, 295, 303, 471
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 8 cut(s) 156, 267, 437, 440, 495, 498, 564, 606
BspANI GGCC 6 cut(s) 107, 140, 217, 392, 501, 540
BspCNI CTCAG 2 cut(s) 9, 204
BspLI GGNNCC 2 cut(s) 108, 622
BspMI ACCTGC 2 cut(s) 51, 72
BspPI GGATC 4 cut(s) 29, 303, 311, 466
BspT107I GGYRCC 1 cut(s) 620
BspTNI GGTCTC 1 cut(s) 44
BsrBI CCGCTC 2 cut(s) 267, 606
BsrFI RCCGGY 2 cut(s) 117, 623
BssAI RCCGGY 2 cut(s) 117, 623
BssECI CCNNGG 4 cut(s) 141, 206, 490, 502
BssMI GATC 4 cut(s) 34, 295, 303, 471
BssT1I CCWWGG 2 cut(s) 141, 490
Bst2UI CCWGG 3 cut(s) 104, 207, 503
Bst4CI ACNGT 2 cut(s) 418, 424
BstAPI GCANNNNNTGC 1 cut(s) 64
BstC8I GCNNGC 1 cut(s) 590
BstDEI CTNAG 2 cut(s) 17, 212
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 3 cut(s) 205, 324, 409
BstKTI GATC 4 cut(s) 37, 298, 306, 474
BstMAI GTCTC 2 cut(s) 44, 164
BstMBI GATC 4 cut(s) 34, 295, 303, 471
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 8 cut(s) 64, 146, 155, 445, 454, 492, 507, 598
BstNI CCWGG 3 cut(s) 104, 207, 503
BstSCI CCNGG 4 cut(s) 102, 126, 205, 501
BstV1I GCAGC 3 cut(s) 133, 345, 495
BstX2I RGATCY 1 cut(s) 471
BstXI CCANNNNNNTGG 1 cut(s) 46
BstYI RGATCY 1 cut(s) 471
BstZI CGGCCG 1 cut(s) 390
Bsu36I CCTNAGG 1 cut(s) 17
BsuRI GGCC 6 cut(s) 107, 140, 217, 392, 501, 540
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 3 cut(s) 205, 324, 409
BveI ACCTGC 2 cut(s) 51, 72
Cac8I GCNNGC 1 cut(s) 590
Cfr10I RCCGGY 2 cut(s) 117, 623
Cfr13I GGNCC 1 cut(s) 106
Csp6I GTAC 3 cut(s) 121, 184, 310
CviAII CATG 2 cut(s) 142, 584
CviQI GTAC 3 cut(s) 121, 184, 310
DdeI CTNAG 2 cut(s) 17, 212
DpnI GATC 4 cut(s) 36, 297, 305, 473
DpnII GATC 4 cut(s) 34, 295, 303, 471
EaeI YGGCCR 3 cut(s) 390, 499, 538
EagI CGGCCG 1 cut(s) 390
EclXI CGGCCG 1 cut(s) 390
Eco130I CCWWGG 2 cut(s) 141, 490
Eco31I GGTCTC 1 cut(s) 44
Eco52I CGGCCG 1 cut(s) 390
Eco81I CCTNAGG 1 cut(s) 17
Eco88I CYCGRG 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 106
EcoRII CCWGG 3 cut(s) 102, 205, 501
EcoT14I CCWWGG 2 cut(s) 141, 490
ErhI CCWWGG 2 cut(s) 141, 490
FaeI CATG 2 cut(s) 145, 587
FaiI YATR 4 cut(s) 143, 224, 585, 633
FatI CATG 2 cut(s) 141, 583
FauI CCCGC 2 cut(s) 163, 260
Fnu4HI GCNGC 7 cut(s) 147, 359, 437, 440, 484, 496, 499
FokI GGATG 3 cut(s) 212, 331, 416
Fsp4HI GCNGC 7 cut(s) 147, 359, 437, 440, 484, 496, 499
GluI GCNGC 7 cut(s) 147, 359, 437, 440, 484, 496, 499
GsaI CCCAGC 1 cut(s) 529
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 6 cut(s) 107, 140, 217, 392, 501, 540
HapII CCGG 5 cut(s) 118, 127, 218, 362, 624
Hin1II CATG 2 cut(s) 145, 587
HpaII CCGG 5 cut(s) 118, 127, 218, 362, 624
HphI GGTGA 2 cut(s) 274, 578
Hpy188I TCNGA 2 cut(s) 79, 471
Hpy188III TCNNGA 4 cut(s) 334, 346, 401, 475
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 6 cut(s) 130, 160, 280, 527, 629, 641
HpyCH4III ACNGT 2 cut(s) 418, 424
HpyCH4IV ACGT 4 cut(s) 123, 186, 252, 550
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 8 cut(s) 64, 146, 155, 445, 454, 492, 507, 598
HpyF3I CTNAG 2 cut(s) 17, 212
HpySE526I ACGT 4 cut(s) 123, 186, 252, 550
Hsp92II CATG 2 cut(s) 145, 587
Kzo9I GATC 4 cut(s) 34, 295, 303, 471
LmnI GCTCC 1 cut(s) 613
Lsp1109I GCAGC 3 cut(s) 133, 345, 495
LweI GCATC 1 cut(s) 169
MaeII ACGT 4 cut(s) 123, 186, 252, 550
MaeIII GTNAC 2 cut(s) 418, 653
MalI GATC 4 cut(s) 36, 297, 305, 473
MbiI CCGCTC 2 cut(s) 267, 606
MboI GATC 4 cut(s) 34, 295, 303, 471
MboII GAAGA 5 cut(s) 247, 289, 442, 463, 542
MflI RGATCY 1 cut(s) 471
MlsI TGGCCA 1 cut(s) 540
MluNI TGGCCA 1 cut(s) 540
MmeI TCCRAC 1 cut(s) 102
Mox20I TGGCCA 1 cut(s) 540
MscI TGGCCA 1 cut(s) 540
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 540
MspI CCGG 5 cut(s) 118, 127, 218, 362, 624
MspR9I CCNGG 4 cut(s) 104, 128, 207, 503
MvaI CCWGG 3 cut(s) 104, 207, 503
MwoI GCNNNNNNNGC 8 cut(s) 64, 146, 155, 445, 454, 492, 507, 598
NciI CCSGG 1 cut(s) 128
NcoI CCATGG 1 cut(s) 141
NdeII GATC 4 cut(s) 34, 295, 303, 471
NlaIII CATG 2 cut(s) 145, 587
NlaIV GGNNCC 2 cut(s) 108, 622
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 2 cut(s) 418, 653
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 75
Pfl23II CGTACG 1 cut(s) 183
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 7 cut(s) 148, 360, 438, 441, 485, 497, 500
PshBI ATTAAT 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 102, 205, 501
PspFI CCCAGC 1 cut(s) 525
PspGI CCWGG 3 cut(s) 102, 205, 501
PspLI CGTACG 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 108, 622
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 1 cut(s) 471
RsaI GTAC 3 cut(s) 122, 185, 311
RsaNI GTAC 3 cut(s) 121, 184, 310
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 7 cut(s) 147, 359, 437, 440, 484, 496, 499
Sau3AI GATC 4 cut(s) 34, 295, 303, 471
Sau96I GGNCC 1 cut(s) 106
ScaI AGTACT 1 cut(s) 311
ScrFI CCNGG 4 cut(s) 104, 128, 207, 503
SfaNI GCATC 1 cut(s) 169
SmlI CTYRAG 2 cut(s) 313, 344
SmoI CTYRAG 2 cut(s) 313, 344
SsiI CCGC 8 cut(s) 156, 267, 437, 440, 495, 498, 564, 606
StyD4I CCNGG 4 cut(s) 102, 126, 205, 501
StyI CCWWGG 2 cut(s) 141, 490
TaaI ACNGT 2 cut(s) 418, 424
TaiI ACGT 4 cut(s) 126, 189, 255, 553
TaqI TCGA 2 cut(s) 33, 69
TatI WGTACW 1 cut(s) 309
TauI GCSGC 4 cut(s) 439, 442, 498, 501
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseFI GTSAC 2 cut(s) 418, 653
TseI GCWGC 3 cut(s) 146, 358, 483
Tsp45I GTSAC 2 cut(s) 418, 653
TspDTI ATGAA 1 cut(s) 648
VspI ATTAAT 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.