Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001449

Physical Location & Seq

Genomic Coordinates Reverse (-)

5470111 .. 5470842

Gene Structure 732 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1068700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 732 bp

ATGGAGGTGATGAGCCTGAGGAATATTGGGTTCGACCCCAACAACCTTCTCGAGACCCTGCACGACTTGCTCGACACCTCCGACGAGCAGAATCATCAGAGCCACCACGCCCCTTCGCGGCAGTACGTCCGCGAGGCCAAGGCCATGGCTGCCACGCCCGCGGACGTGAAGGAGACTCAAAATGCCTATGTATTCGTGGTGGACGTGCCTGGGCTGAGGCCGGATATGATTGATGTGAAGATTGAAGACGACAACGTGCTGGTGGTGGGTGGGGAGAGGAGGAGAGAGAAGGAAAAGGATCAGGGGGTCAAGTATTTGAGGATGGAGAGGAGGCTCGGGAAGTATTTGAAGAAGTTTGTGCTGCCGGACAATGTAGATATTGAGAAAATTTCGGCCGAGTGTCAGGACGGGGTGCTGACTATCACTGTGGTGAAGAGGCCGCCGCCAGAGCCTAAGAAACCCAAGGCTATTCAAGTGCAAATAAGCAGCGGTCAAGGCGACCGCCAAGGAGGCGTACATGTCGAAAGCGGTGGTCAAGGATGGCAAGGTGGTACCCAAGGGAAATATGGTGGGCAAGGCAGCGAACAAGGTGGGGGGCAAGGTGGTGGTCAAGAATCACAAGGCGGACGCCTAGGTGGTGGTCAAGAATGGCAAGCCGGCCGCCAAGGTGGAGGCCAAGGTGGCCAAGGAGATCAAGGGAATGCTGGGGCAGCGCGGCGCGTCCTCCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

HSP20

Protein Analysis

244

Amino Acids

26.17

Weight (kDa)

6.24

Isoelectric Point (pI)

55.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP20	PF00011	55 - 160	7.5e-29	Hsp20/alpha crystallin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001449)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12020 AT5G12030
fragaria_vesca	FvH4_2g31371
malus_domestica	MD00G1181700.v1.1 MD08G1068000.v1.1 MD08G1068200.v1.1 MD08G1068300.v1.1 MD08G1068500.v1.1 MD08G1068700.v1.1 MD08G1068800.v1.1 MD15G1053500.v1.1 MD15G1053600.v1.1 MD15G1053800.v1.1
prunus_persica	Prupe.1G407100_v2.0.a1 Prupe.1G407200_v2.0.a1 Prupe.1G407300_v2.0.a1
pyrus_communis	pycom08g05660 pycom08g05670
rosa_chinensis	RchiOBHm_Chr6g0311451 RchiOBHm_Chr6g0311461 RchiOBHm_Chr6g0311471
rosa_laevigata	RLG00000010403 RLG00000010404 RLG00000010405
rosa_multiflora	Rmu_co8421495.1_g000001 Rmu_sc0006915.1_g000010 Rmu_sc0006915.1_g000011 Rmu_sc0006915.1_g000012 Rmu_sc0013619.1_g000003 Rmu_sc0013619.1_g000004 Rmu_sc0013619.1_g000006 Rmu_sc0015790.1_g000003 Rmu_sc0015790.1_g000004
rosa_roxburghii	Rroxscaffold_7G00157590 Rroxscaffold_7G00157600
rosa_rugosa	Rorug06G0389100 Rorug06G0389100 Rorug06G0389200
rosa_samantha	Rh2AG205100 Rh2BG216900 Rh2DG211400 Rh6AG503500 Rh6AG503700 Rh6BG514200 Rh6BG514300 Rh6CG520000 Rh6CG520100 Rh6DG506100
rosa_wichuraiana	Rw2G016340 Rw6G043850 Rw6G043860 Rw6G043870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	551
AccB1I	GGYRCC	1 cut(s)	551
AccII	CGCG	5 cut(s)	118, 132, 161, 715, 720
AclWI	GGATC	1 cut(s)	306
AcoI	YGGCCR	3 cut(s)	393, 658, 682
AcsI	RAATTY	1 cut(s)	387
AcyI	GRCGYC	1 cut(s)	628
AfaI	GTAC	3 cut(s)	125, 516, 553
AfiI	CCNNNNNNNGG	1 cut(s)	117
AflIII	ACRYGT	1 cut(s)	517
AgsI	TTSAA	3 cut(s)	245, 349, 473
AjiI	CACGTC	2 cut(s)	166, 205
AjnI	CCWGG	1 cut(s)	208
AleI	CACNNNNGTG	1 cut(s)	428
Alw26I	GTCTC	2 cut(s)	47, 167
AlwI	GGATC	1 cut(s)	306
Ama87I	CYCGRG	2 cut(s)	50, 335
AoxI	GGCC	8 cut(s)	135, 141, 218, 393, 437, 658, 673, 682
ApeKI	GCWGC	5 cut(s)	149, 361, 486, 579, 710
ApoI	RAATTY	1 cut(s)	387
Asp718I	GGTACC	1 cut(s)	551
AspA2I	CCTAGG	1 cut(s)	631
AspLEI	GCGC	2 cut(s)	715, 720
AsuHPI	GGTGA	2 cut(s)	19, 442
AvaI	CYCGRG	2 cut(s)	50, 335
AvrII	CCTAGG	1 cut(s)	631
AxyI	CCTNAGG	1 cut(s)	17
BalI	TGGCCA	1 cut(s)	684
BanI	GGYRCC	1 cut(s)	551
BbsI	GAAGAC	1 cut(s)	252
BbvCI	CCTCAGC	1 cut(s)	215
BbvI	GCAGC	5 cut(s)	136, 348, 498, 591, 722
BccI	CCATC	2 cut(s)	316, 534
BciT130I	CCWGG	1 cut(s)	210
BcoDI	GTCTC	2 cut(s)	47, 167
BfaI	CTAG	1 cut(s)	632
BglI	GCCNNNNNGGC	2 cut(s)	510, 681
BlnI	CCTAGG	1 cut(s)	631
Bme1390I	CCNGG	1 cut(s)	210
BmeT110I	CYCGRG	2 cut(s)	50, 335
BmgBI	CACGTC	2 cut(s)	166, 205
BmiI	GGNNCC	1 cut(s)	553
BmrFI	CCNGG	1 cut(s)	210
BpiI	GAAGAC	1 cut(s)	252
Bpu10I	CCTNAGC	1 cut(s)	215
BsaHI	GRCGYC	1 cut(s)	628
BsaI	GGTCTC	1 cut(s)	47
Bsc4I	CCNNNNNNNGG	1 cut(s)	117
Bse118I	RCCGGY	1 cut(s)	656
Bse21I	CCTNAGG	1 cut(s)	17
BseBI	CCWGG	1 cut(s)	210
BseGI	GGATG	2 cut(s)	327, 545
BseLI	CCNNNNNNNGG	1 cut(s)	117
BseMII	CTCAG	2 cut(s)	8, 206
BseRI	GAGGAG	3 cut(s)	292, 295, 343
BseX3I	CGGCCG	2 cut(s)	393, 658
BseXI	GCAGC	5 cut(s)	136, 348, 498, 591, 722
BseYI	CCCAGC	1 cut(s)	704
BsgI	GTGCAG	1 cut(s)	44
Bsh1236I	CGCG	5 cut(s)	118, 132, 161, 715, 720
Bsh1285I	CGRYCG	3 cut(s)	396, 502, 661
BshFI	GGCC	8 cut(s)	137, 143, 220, 395, 439, 660, 675, 684
BshNI	GGYRCC	1 cut(s)	551
BsiEI	CGRYCG	3 cut(s)	396, 502, 661
BsiHKCI	CYCGRG	2 cut(s)	50, 335
BsiSI	CCGG	3 cut(s)	221, 365, 657
BslI	CCNNNNNNNGG	1 cut(s)	117
BsmAI	GTCTC	2 cut(s)	47, 167
BsmI	GAATGC	1 cut(s)	706
BsnI	GGCC	8 cut(s)	137, 143, 220, 395, 439, 660, 675, 684
Bso31I	GGTCTC	1 cut(s)	47
BsoBI	CYCGRG	2 cut(s)	50, 335
Bsp143I	GATC	2 cut(s)	298, 691
Bsp19I	CCATGG	1 cut(s)	144
BspANI	GGCC	8 cut(s)	137, 143, 220, 395, 439, 660, 675, 684
BspCNI	CTCAG	2 cut(s)	9, 207
BspFNI	CGCG	5 cut(s)	118, 132, 161, 715, 720
BspLI	GGNNCC	1 cut(s)	553
BspPI	GGATC	1 cut(s)	306
BspT107I	GGYRCC	1 cut(s)	551
BspTNI	GGTCTC	1 cut(s)	47
BsrFI	RCCGGY	1 cut(s)	656
BssAI	RCCGGY	1 cut(s)	656
BssMI	GATC	2 cut(s)	298, 691
BssNI	GRCGYC	1 cut(s)	628
BssT1I	CCWWGG	9 cut(s)	138, 144, 462, 505, 556, 631, 664, 676, 685
Bst2UI	CCWGG	1 cut(s)	210
Bst4CI	ACNGT	1 cut(s)	427
Bst6I	CTCTTC	1 cut(s)	428
BstACI	GRCGYC	1 cut(s)	628
BstAPI	GCANNNNNTGC	1 cut(s)	67
BstC8I	GCNNGC	3 cut(s)	159, 654, 658
BstDEI	CTNAG	3 cut(s)	17, 215, 453
BstDSI	CCRYGG	2 cut(s)	144, 159
BstF5I	GGATG	2 cut(s)	327, 545
BstFNI	CGCG	5 cut(s)	118, 132, 161, 715, 720
BstHHI	GCGC	2 cut(s)	715, 720
BstKTI	GATC	2 cut(s)	301, 694
BstMAI	GTCTC	2 cut(s)	47, 167
BstMBI	GATC	2 cut(s)	298, 691
BstMCI	CGRYCG	3 cut(s)	396, 502, 661
BstMWI	GCNNNNNNNGC	8 cut(s)	67, 149, 158, 448, 495, 510, 681, 710
BstNI	CCWGG	1 cut(s)	210
BstNSI	RCATGY	1 cut(s)	521
BstSCI	CCNGG	1 cut(s)	208
BstUI	CGCG	5 cut(s)	118, 132, 161, 715, 720
BstV1I	GCAGC	5 cut(s)	136, 348, 498, 591, 722
BstV2I	GAAGAC	1 cut(s)	252
BstXI	CCANNNNNNTGG	1 cut(s)	145
BstZI	CGGCCG	2 cut(s)	393, 658
Bsu36I	CCTNAGG	1 cut(s)	17
BsuRI	GGCC	8 cut(s)	137, 143, 220, 395, 439, 660, 675, 684
BtgI	CCRYGG	2 cut(s)	144, 159
BtrI	CACGTC	2 cut(s)	166, 205
BtsCI	GGATG	2 cut(s)	327, 545
BtsIMutI	CAGTG	1 cut(s)	423
Cac8I	GCNNGC	3 cut(s)	159, 654, 658
CfoI	GCGC	2 cut(s)	715, 720
Cfr10I	RCCGGY	1 cut(s)	656
Cfr42I	CCGCGG	1 cut(s)	162
CseI	GACGC	2 cut(s)	636, 709
Csp6I	GTAC	3 cut(s)	124, 515, 552
CviAII	CATG	3 cut(s)	145, 518, 729
CviQI	GTAC	3 cut(s)	124, 515, 552
DdeI	CTNAG	3 cut(s)	17, 215, 453
DpnI	GATC	2 cut(s)	300, 693
DpnII	GATC	2 cut(s)	298, 691
EaeI	YGGCCR	3 cut(s)	393, 658, 682
EagI	CGGCCG	2 cut(s)	393, 658
Eam1104I	CTCTTC	1 cut(s)	428
EarI	CTCTTC	1 cut(s)	428
EciI	GGCGGA	1 cut(s)	639
EclXI	CGGCCG	2 cut(s)	393, 658
Eco130I	CCWWGG	9 cut(s)	138, 144, 462, 505, 556, 631, 664, 676, 685
Eco31I	GGTCTC	1 cut(s)	47
Eco52I	CGGCCG	2 cut(s)	393, 658
Eco81I	CCTNAGG	1 cut(s)	17
Eco88I	CYCGRG	2 cut(s)	50, 335
EcoRII	CCWGG	1 cut(s)	208
EcoT14I	CCWWGG	9 cut(s)	138, 144, 462, 505, 556, 631, 664, 676, 685
ErhI	CCWWGG	9 cut(s)	138, 144, 462, 505, 556, 631, 664, 676, 685
FaeI	CATG	3 cut(s)	148, 521, 732
FaiI	YATR	6 cut(s)	146, 189, 227, 519, 567, 730
FatI	CATG	3 cut(s)	144, 517, 728
FauI	CCCGC	1 cut(s)	166
FokI	GGATG	2 cut(s)	334, 552
FspBI	CTAG	1 cut(s)	632
GlaI	GCGC	2 cut(s)	714, 719
GsaI	CCCAGC	1 cut(s)	708
HaeIII	GGCC	8 cut(s)	137, 143, 220, 395, 439, 660, 675, 684
HapII	CCGG	3 cut(s)	221, 365, 657
HgaI	GACGC	2 cut(s)	636, 709
HhaI	GCGC	2 cut(s)	715, 720
Hin1I	GRCGYC	1 cut(s)	628
Hin1II	CATG	3 cut(s)	148, 521, 732
Hin6I	GCGC	2 cut(s)	713, 718
HinP1I	GCGC	2 cut(s)	713, 718
HinfI	GANTC	3 cut(s)	91, 175, 614
HpaII	CCGG	3 cut(s)	221, 365, 657
HphI	GGTGA	2 cut(s)	19, 442
Hpy166II	GTNNAC	1 cut(s)	202
Hpy188I	TCNGA	2 cut(s)	82, 99
Hpy188III	TCNNGA	6 cut(s)	50, 52, 337, 404, 611, 644
Hpy8I	GTNNAC	1 cut(s)	202
Hpy99I	CGWCG	1 cut(s)	86
HpyAV	CCTTC	4 cut(s)	56, 123, 163, 283
HpyCH4III	ACNGT	1 cut(s)	427
HpyCH4IV	ACGT	4 cut(s)	126, 165, 204, 255
HpyCH4V	TGCA	2 cut(s)	61, 478
HpyF10VI	GCNNNNNNNGC	8 cut(s)	67, 149, 158, 448, 495, 510, 681, 710
HpyF3I	CTNAG	3 cut(s)	17, 215, 453
HpySE526I	ACGT	4 cut(s)	126, 165, 204, 255
Hsp92I	GRCGYC	1 cut(s)	628
Hsp92II	CATG	3 cut(s)	148, 521, 732
HspAI	GCGC	2 cut(s)	713, 718
KpnI	GGTACC	1 cut(s)	555
KroI	GCCGGC	1 cut(s)	656
KroNI	GCCGGC	1 cut(s)	658
KspI	CCGCGG	1 cut(s)	162
Kzo9I	GATC	2 cut(s)	298, 691
Lsp1109I	GCAGC	5 cut(s)	136, 348, 498, 591, 722
MaeI	CTAG	1 cut(s)	632
MaeII	ACGT	4 cut(s)	126, 165, 204, 255
MalI	GATC	2 cut(s)	300, 693
MboI	GATC	2 cut(s)	298, 691
MboII	GAAGA	4 cut(s)	250, 257, 361, 445
MlsI	TGGCCA	1 cut(s)	684
MluCI	AATT	1 cut(s)	387
MluNI	TGGCCA	1 cut(s)	684
MlyI	GAGTC	1 cut(s)	169
MmeI	TCCRAC	1 cut(s)	105
Mox20I	TGGCCA	1 cut(s)	684
MroNI	GCCGGC	1 cut(s)	656
MscI	TGGCCA	1 cut(s)	684
MslI	CAYNNNNRTG	1 cut(s)	428
Msp20I	TGGCCA	1 cut(s)	684
MspA1I	CMGCKG	2 cut(s)	161, 489
MspI	CCGG	3 cut(s)	221, 365, 657
MspR9I	CCNGG	1 cut(s)	210
MteI	GCGCNGCGC	1 cut(s)	716
Mva1269I	GAATGC	1 cut(s)	706
MvaI	CCWGG	1 cut(s)	210
MvnI	CGCG	5 cut(s)	118, 132, 161, 715, 720
MwoI	GCNNNNNNNGC	8 cut(s)	67, 149, 158, 448, 495, 510, 681, 710
NaeI	GCCGGC	1 cut(s)	658
NcoI	CCATGG	1 cut(s)	144
NdeII	GATC	2 cut(s)	298, 691
NgoMIV	GCCGGC	1 cut(s)	656
NlaIII	CATG	3 cut(s)	148, 521, 732
NlaIV	GGNNCC	1 cut(s)	553
NmeAIII	GCCGAG	1 cut(s)	421
NspI	RCATGY	1 cut(s)	521
OliI	CACNNNNGTG	1 cut(s)	428
PaeR7I	CTCGAG	1 cut(s)	50
PciI	ACATGT	1 cut(s)	517
PcsI	WCGNNNNNNNCGW	3 cut(s)	69, 78, 201
PctI	GAATGC	1 cut(s)	706
PdiI	GCCGGC	1 cut(s)	658
PfeI	GAWTC	2 cut(s)	91, 614
PleI	GAGTC	1 cut(s)	169
PpsI	GAGTC	1 cut(s)	169
PscI	ACATGT	1 cut(s)	517
Psp6I	CCWGG	1 cut(s)	208
PspFI	CCCAGC	1 cut(s)	704
PspGI	CCWGG	1 cut(s)	208
PspN4I	GGNNCC	1 cut(s)	553
RsaI	GTAC	3 cut(s)	125, 516, 553
RsaNI	GTAC	3 cut(s)	124, 515, 552
RseI	CAYNNNNRTG	1 cut(s)	428
SacII	CCGCGG	1 cut(s)	162
Sau3AI	GATC	2 cut(s)	298, 691
SchI	GAGTC	1 cut(s)	169
ScrFI	CCNGG	1 cut(s)	210
SfiI	GGCCNNNNNGGCC	1 cut(s)	681
Sfr274I	CTCGAG	1 cut(s)	50
Sfr303I	CCGCGG	1 cut(s)	162
SgrBI	CCGCGG	1 cut(s)	162
SlaI	CTCGAG	1 cut(s)	50
SmiMI	CAYNNNNRTG	1 cut(s)	428
SmlI	CTYRAG	1 cut(s)	50
SmoI	CTYRAG	1 cut(s)	50
Sse9I	AATT	1 cut(s)	387
SspI	AATATT	1 cut(s)	25
SspMI	CTAG	1 cut(s)	632
StyD4I	CCNGG	1 cut(s)	208
StyI	CCWWGG	9 cut(s)	138, 144, 462, 505, 556, 631, 664, 676, 685
TaaI	ACNGT	1 cut(s)	427
TaiI	ACGT	4 cut(s)	129, 168, 207, 258
TaqI	TCGA	4 cut(s)	33, 51, 72, 522
TasI	AATT	1 cut(s)	387
TauI	GCSGC	5 cut(s)	121, 442, 445, 663, 718
TfiI	GAWTC	2 cut(s)	91, 614
TscAI	CASTG	1 cut(s)	430
TseI	GCWGC	5 cut(s)	149, 361, 486, 579, 710
TspRI	CASTG	1 cut(s)	430
XapI	RAATTY	1 cut(s)	387
XceI	RCATGY	1 cut(s)	521
XcmI	CCANNNNNNNNNTGG	1 cut(s)	563
XhoI	CTCGAG	1 cut(s)	50
XmaJI	CCTAGG	1 cut(s)	631
XspI	CTAG	1 cut(s)	632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.