MD15G1053900.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB NOP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009262
Physical Location & Seq
Reverse (-)
3676214 .. 3680025
3812 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1053900.v1.1.491

Sequence Viewer

Length: 1518 bp
ATGGCGCGCCTAAAGCACAAAGCCGGCCCGCCGCCTGCAGCCGCCGATAAGTCAGAAAACCGGCGTCGTTTAAACATGGAGAGGTCGGCTTACTTCTCCCGGAGAGAGGCTGCGAAGGTATTGAAGCTCGTCCTCCAAGGCGACGCCAAGAAGCGGTCCGTCGGCACCATCAAGTCGCTCGTTTACAGTCCCTCAGTCAGGAACAAGAAAGGCACTTTCGCTTTGGTTTGCCAAACCCTAAAATATCTTCAGATTATCCAAGAGGTCTTGAATGCTGCCGAAGTACTGAATAGCAAGTGGAAGAAGCAAGATGAGTTGGTTTACATAGTCACCTACGACATCCTTTTCGGTCAGGTGAGTTCATCTGCAATAGGTGATGCGGAGAAGTATCTCATGCGTCGCCAAGAAGCTCTGCAGTCCGCTCTGGCTCGGCTTTTGGTGAAGAAAAAAGTGAAGAAAGTTGAAGACTTGATAGCTCTTTATGAAATTCCCGATATCTCAATACCTCGTCATGTTCGAGTAAATACTCTGAAAATGGATGTTGACACTGCCTTACTCGAATTAAAGAAGCAATTTCAGGTTCAAAAGGATGACCTGGTTCCTGACTTGTTGATTCTTCCACCGGGTAGTGATCTTCACGATCATCCTCTAATAAAGGATGGAAGTATTTTTATGCAAGGAAAGGCAAGCTCTATGGTGGCTGCAGCCCTTGCTCCGAAACCAGGATGGGAGGTTCTTGATGCATGCTCGGCTCCTGGAAACAAAACTGTGCACCTTGCAGCTCTTATGAGAGGAGAGGGAAAAGTGATAGCATGTGAACTGAATGAAGAAAGGGTTAAACGTTTAAAAGAGACTATCAGACTTTCTGGTGCTTCTAATATTAAGGTTGTGCATGGAGATTTCTTAGACTTAAACCCTGAAGATCCTCAATTTTCCAAGGTCCGGGCCATTCTTTTAGATCCTTCCTGCTCTGGATCTGGGACTGCTTCTGCAAGATTAGATCATTTGCTCCCATCTTCTGCTCCAGGTCAGGGTGCCGATTTTGCCAGCACAGAAAGACTTAACAAGCTCGCCGCCTTTCAGAGAAAAGCACTGGCTCATGCTTTATCTTTTCCGGAGGTGGAGAGAGTCGTTTACAGCACATGCTCTGTCAACCAAGTCGAAAACGAAGATGTCGTTAAATCTGTGCTACCACTTGCTGCATCCAATGGTTTCCAACTGGAAACCCCCTTCCCGAAATGGCACCGCCGTGGTCTCCCCGTTATCGAGGGCGCCGAATGCTTGATTAGGACCGATCCGAAGGAGGACAAAGAAGGCTTCTTCATCGCTCTGTTTTCCAGGAGCTCCAGCAACCAACAGGAGGCGCCTAATGGAACCGGCAGAGACGCCACCGAAGCCTCGAAGAGGCAGAGAAATTATGCAGTACCTCATCTCAATCTGTTTAAAACGTGGACACATGCGCAACGGATGTGGCGTAATAATTCCAAACACATTCACAGTCATTGCCATTGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltr_RsmB-F

Protein Analysis

506

Amino Acids

56.11

Weight (kDa)

9.41

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NSUN5_N PF21153 72 - 158 1.3e-17 NOL1/NOP2/Sun domain family member 5, N-terminal domain
NSUN5_fdxn-like PF21148 168 - 226 1.8e-07 NOL1/NOP2/Sun domain family member 5, ferredoxin-like domain
Ubie_methyltran PF01209 233 - 312 7.8e-06 ubiE/COQ5 methyltransferase family
Methyltr_RsmB-F PF01189 235 - 445 5e-26 16S rRNA methyltransferase RsmB/F
Methyltransf_31 PF13847 240 - 308 6.8e-06 Methyltransferase domain
Methyltransf_25 PF13649 245 - 316 3.9e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1461
AccB1I GGYRCC 5 cut(s) 164, 1034, 1242, 1271, 1363
AccBSI CCGCTC 1 cut(s) 422
AccII CGCG 1 cut(s) 7
AccIII TCCGGA 1 cut(s) 1114
AciI CCGC 8 cut(s) 29, 32, 42, 154, 380, 420, 1074, 1246
AclI AACGTT 1 cut(s) 841
AclWI GGATC 4 cut(s) 917, 953, 982, 1289
AcsI RAATTY 1 cut(s) 486
AcuI CTGAAG 2 cut(s) 233, 939
AcyI GRCGYC 5 cut(s) 64, 144, 1272, 1364, 1386
AfaI GTAC 2 cut(s) 285, 1425
AfiI CCNNNNNNNGG 8 cut(s) 106, 153, 198, 722, 942, 1031, 1360, 1404
AgsI TTSAA 4 cut(s) 124, 271, 464, 584
AjnI CCWGG 5 cut(s) 594, 721, 754, 1024, 1337
AleI CACNNNNGTG 1 cut(s) 1248
AloI GAACNNNNNNTCC 2 cut(s) 717, 749
AluBI AGCT 7 cut(s) 127, 410, 476, 690, 782, 1069, 1344
AluI AGCT 7 cut(s) 127, 410, 476, 690, 782, 1069, 1344
Alw21I GWGCWC 2 cut(s) 774, 1346
Alw26I GTCTC 3 cut(s) 845, 1259, 1377
Alw44I GTGCAC 1 cut(s) 770
AlwI GGATC 4 cut(s) 917, 953, 982, 1289
Aor13HI TCCGGA 1 cut(s) 1114
AoxI GGCC 2 cut(s) 25, 945
ApaLI GTGCAC 1 cut(s) 770
ApeKI GCWGC 7 cut(s) 38, 110, 275, 701, 704, 779, 1199
ApoI RAATTY 1 cut(s) 486
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AscI GGCGCGCC 1 cut(s) 5
AspLEI GCGC 5 cut(s) 7, 9, 1274, 1366, 1462
AspS9I GGNCC 5 cut(s) 26, 156, 940, 945, 1290
AsuC2I CCSGG 3 cut(s) 100, 624, 944
AsuHPI GGTGA 4 cut(s) 322, 367, 386, 451
AvaII GGWCC 3 cut(s) 156, 940, 1290
BaeGI GKGCMC 1 cut(s) 774
BanI GGYRCC 5 cut(s) 164, 1034, 1242, 1271, 1363
BanII GRGCYC 1 cut(s) 1346
BbsI GAAGAC 1 cut(s) 471
Bbv12I GWGCWC 2 cut(s) 774, 1346
BbvI GCAGC 7 cut(s) 50, 97, 262, 688, 716, 791, 1186
BccI CCATC 4 cut(s) 176, 653, 720, 1021
BceAI ACGGC 1 cut(s) 1233
BciT130I CCWGG 5 cut(s) 596, 723, 756, 1026, 1339
BcnI CCSGG 3 cut(s) 100, 624, 944
BcoDI GTCTC 3 cut(s) 845, 1259, 1377
BfmI CTRYAG 3 cut(s) 36, 413, 702
BfoI RGCGCY 2 cut(s) 1275, 1367
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 8 cut(s) 100, 596, 624, 723, 756, 944, 1026, 1339
Bme18I GGWCC 3 cut(s) 156, 940, 1290
BmgT120I GGNCC 5 cut(s) 26, 156, 940, 945, 1290
BmiI GGNNCC 8 cut(s) 166, 600, 753, 1036, 1244, 1273, 1365, 1375
BmrFI CCNGG 8 cut(s) 100, 596, 624, 723, 756, 944, 1026, 1339
BmsI GCATC 3 cut(s) 367, 730, 1211
BpiI GAAGAC 1 cut(s) 471
BpmI CTGGAG 2 cut(s) 1008, 1330
BpuMI CCSGG 3 cut(s) 100, 624, 944
BsaHI GRCGYC 5 cut(s) 64, 144, 1272, 1364, 1386
BsaI GGTCTC 1 cut(s) 1259
BsaJI CCNNGG 3 cut(s) 136, 936, 1249
BsaWI WCCGGW 1 cut(s) 1114
Bsc4I CCNNNNNNNGG 8 cut(s) 106, 153, 198, 722, 942, 1031, 1360, 1404
Bse118I RCCGGY 3 cut(s) 23, 60, 1376
Bse1I ACTGG 2 cut(s) 1098, 1224
Bse3DI GCAATG 1 cut(s) 1501
BseAI TCCGGA 1 cut(s) 1114
BseBI CCWGG 5 cut(s) 596, 723, 756, 1026, 1339
BseDI CCNNGG 3 cut(s) 136, 936, 1249
BseGI GGATG 8 cut(s) 339, 544, 595, 643, 664, 731, 1202, 1473
BseLI CCNNNNNNNGG 8 cut(s) 106, 153, 198, 722, 942, 1031, 1360, 1404
BseMI GCAATG 1 cut(s) 1501
BseMII CTCAG 1 cut(s) 207
BseNI ACTGG 2 cut(s) 1098, 1224
BsePI GCGCGC 1 cut(s) 5
BseRI GAGGAG 1 cut(s) 807
BseSI GKGCMC 1 cut(s) 774
BseXI GCAGC 7 cut(s) 50, 97, 262, 688, 716, 791, 1186
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 2 cut(s) 27, 947
BshNI GGYRCC 5 cut(s) 164, 1034, 1242, 1271, 1363
BsiHKAI GWGCWC 2 cut(s) 774, 1346
BsiSI CCGG 7 cut(s) 24, 61, 100, 623, 943, 1115, 1377
BslFI GGGAC 2 cut(s) 174, 994
BslI CCNNNNNNNGG 8 cut(s) 106, 153, 198, 722, 942, 1031, 1360, 1404
BsmAI GTCTC 3 cut(s) 845, 1259, 1377
BsmBI CGTCTC 1 cut(s) 1377
BsmFI GGGAC 2 cut(s) 174, 994
BsmI GAATGC 2 cut(s) 277, 1283
BsnI GGCC 2 cut(s) 27, 947
Bso31I GGTCTC 1 cut(s) 1259
Bsp1286I GDGCHC 2 cut(s) 774, 1346
Bsp13I TCCGGA 1 cut(s) 1114
Bsp143I GATC 7 cut(s) 631, 640, 922, 958, 974, 1000, 1294
BspACI CCGC 8 cut(s) 29, 32, 42, 154, 380, 420, 1074, 1246
BspANI GGCC 2 cut(s) 27, 947
BspCNI CTCAG 1 cut(s) 206
BspEI TCCGGA 1 cut(s) 1114
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 8 cut(s) 166, 600, 753, 1036, 1244, 1273, 1365, 1375
BspMAI CTGCAG 3 cut(s) 40, 417, 706
BspPI GGATC 4 cut(s) 917, 953, 982, 1289
BspT107I GGYRCC 5 cut(s) 164, 1034, 1242, 1271, 1363
BspTNI GGTCTC 1 cut(s) 1259
BsrBI CCGCTC 1 cut(s) 422
BsrDI GCAATG 1 cut(s) 1501
BsrFI RCCGGY 3 cut(s) 23, 60, 1376
BsrI ACTGG 2 cut(s) 1098, 1224
BssAI RCCGGY 3 cut(s) 23, 60, 1376
BssECI CCNNGG 3 cut(s) 136, 936, 1249
BssHII GCGCGC 1 cut(s) 5
BssMI GATC 7 cut(s) 631, 640, 922, 958, 974, 1000, 1294
BssNI GRCGYC 5 cut(s) 64, 144, 1272, 1364, 1386
BssT1I CCWWGG 2 cut(s) 136, 936
Bst2UI CCWGG 5 cut(s) 596, 723, 756, 1026, 1339
Bst4CI ACNGT 3 cut(s) 188, 769, 1499
Bst6I CTCTTC 1 cut(s) 1397
BstACI GRCGYC 5 cut(s) 64, 144, 1272, 1364, 1386
BstAPI GCANNNNNTGC 1 cut(s) 710
BstC8I GCNNGC 8 cut(s) 7, 25, 29, 36, 688, 745, 1048, 1071
BstDEI CTNAG 2 cut(s) 193, 904
BstDSI CCRYGG 1 cut(s) 1249
BstENI CCTNNNNNAGG 2 cut(s) 196, 1402
BstF5I GGATG 8 cut(s) 339, 544, 595, 643, 664, 731, 1202, 1473
BstFNI CGCG 1 cut(s) 7
BstH2I RGCGCY 2 cut(s) 1275, 1367
BstHHI GCGC 5 cut(s) 7, 9, 1274, 1366, 1462
BstKTI GATC 7 cut(s) 634, 643, 925, 961, 977, 1003, 1297
BstMAI GTCTC 3 cut(s) 845, 1259, 1377
BstMBI GATC 7 cut(s) 631, 640, 922, 958, 974, 1000, 1294
BstMWI GCNNNNNNNGC 6 cut(s) 13, 710, 749, 1043, 1278, 1394
BstNI CCWGG 5 cut(s) 596, 723, 756, 1026, 1339
BstNSI RCATGY 4 cut(s) 747, 816, 1146, 1460
BstSCI CCNGG 8 cut(s) 98, 594, 622, 721, 754, 942, 1024, 1337
BstSFI CTRYAG 3 cut(s) 36, 413, 702
BstSLI GKGCMC 1 cut(s) 774
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 7 cut(s) 50, 97, 262, 688, 716, 791, 1186
BstV2I GAAGAC 1 cut(s) 471
BstX2I RGATCY 3 cut(s) 922, 958, 974
BstYI RGATCY 3 cut(s) 922, 958, 974
BsuRI GGCC 2 cut(s) 27, 947
BtgI CCRYGG 1 cut(s) 1249
BtgZI GCGATG 1 cut(s) 1309
BtsCI GGATG 8 cut(s) 339, 544, 595, 643, 664, 731, 1202, 1473
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 2 cut(s) 546, 1091
Cac8I GCNNGC 8 cut(s) 7, 25, 29, 36, 688, 745, 1048, 1071
CfoI GCGC 5 cut(s) 7, 9, 1274, 1366, 1462
Cfr10I RCCGGY 3 cut(s) 23, 60, 1376
Cfr13I GGNCC 5 cut(s) 26, 156, 940, 945, 1290
CpoI CGGWCCG 1 cut(s) 156
CseI GACGC 4 cut(s) 53, 152, 386, 1394
CsiI ACCWGGT 1 cut(s) 594
Csp6I GTAC 2 cut(s) 284, 1424
CspCI CAANNNNNGTGG 2 cut(s) 1451, 1486
CspI CGGWCCG 1 cut(s) 156
CviAII CATG 9 cut(s) 76, 394, 512, 744, 813, 893, 1100, 1143, 1457
CviQI GTAC 2 cut(s) 284, 1424
DdeI CTNAG 2 cut(s) 193, 904
DinI GGCGCC 2 cut(s) 1273, 1365
DpnI GATC 7 cut(s) 633, 642, 924, 960, 976, 1002, 1296
DpnII GATC 7 cut(s) 631, 640, 922, 958, 974, 1000, 1294
DraI TTTAAA 3 cut(s) 72, 846, 1444
Eam1104I CTCTTC 1 cut(s) 1397
EarI CTCTTC 1 cut(s) 1397
Ecl136II GAGCTC 1 cut(s) 1344
Eco130I CCWWGG 2 cut(s) 136, 936
Eco24I GRGCYC 1 cut(s) 1346
Eco31I GGTCTC 1 cut(s) 1259
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 3 cut(s) 156, 940, 1290
Eco53kI GAGCTC 1 cut(s) 1344
Eco57I CTGAAG 2 cut(s) 233, 939
EcoICRI GAGCTC 1 cut(s) 1344
EcoNI CCTNNNNNAGG 2 cut(s) 196, 1402
EcoRII CCWGG 5 cut(s) 594, 721, 754, 1024, 1337
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 2 cut(s) 136, 936
EcoT22I ATGCAT 1 cut(s) 745
EcoT38I GRGCYC 1 cut(s) 1346
EgeI GGCGCC 2 cut(s) 1273, 1365
EheI GGCGCC 2 cut(s) 1273, 1365
ErhI CCWWGG 2 cut(s) 136, 936
Esp3I CGTCTC 1 cut(s) 1377
FaeI CATG 9 cut(s) 79, 397, 515, 747, 816, 896, 1103, 1146, 1460
FaqI GGGAC 2 cut(s) 174, 994
FatI CATG 9 cut(s) 75, 393, 511, 743, 812, 892, 1099, 1142, 1456
FauI CCCGC 1 cut(s) 36
FokI GGATG 8 cut(s) 326, 551, 602, 630, 671, 738, 1189, 1480
FriOI GRGCYC 1 cut(s) 1346
FspI TGCGCA 1 cut(s) 1461
GlaI GCGC 5 cut(s) 6, 8, 1273, 1365, 1461
GsuI CTGGAG 2 cut(s) 1008, 1330
HaeII RGCGCY 2 cut(s) 1275, 1367
HaeIII GGCC 2 cut(s) 27, 947
HapII CCGG 7 cut(s) 24, 61, 100, 623, 943, 1115, 1377
HgaI GACGC 4 cut(s) 53, 152, 386, 1394
HhaI GCGC 5 cut(s) 7, 9, 1274, 1366, 1462
Hin1I GRCGYC 5 cut(s) 64, 144, 1272, 1364, 1386
Hin1II CATG 9 cut(s) 79, 397, 515, 747, 816, 896, 1103, 1146, 1460
Hin6I GCGC 5 cut(s) 5, 7, 1272, 1364, 1460
HinP1I GCGC 5 cut(s) 5, 7, 1272, 1364, 1460
HincII GTYRAC 2 cut(s) 544, 1153
HindII GTYRAC 2 cut(s) 544, 1153
HinfI GANTC 2 cut(s) 613, 1128
HpaII CCGG 7 cut(s) 24, 61, 100, 623, 943, 1115, 1377
HphI GGTGA 4 cut(s) 322, 367, 386, 451
Hpy166II GTNNAC 8 cut(s) 184, 322, 544, 772, 818, 1135, 1153, 1452
Hpy188I TCNGA 7 cut(s) 55, 252, 531, 717, 860, 1083, 1299
Hpy188III TCNNGA 9 cut(s) 199, 268, 491, 602, 638, 737, 972, 1115, 1234
Hpy8I GTNNAC 8 cut(s) 184, 322, 544, 772, 818, 1135, 1153, 1452
Hpy99I CGWCG 4 cut(s) 69, 146, 164, 402
HpyAV CCTTC 5 cut(s) 109, 972, 1240, 1294, 1307
HpyCH4III ACNGT 3 cut(s) 188, 769, 1499
HpyCH4IV ACGT 2 cut(s) 841, 1448
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 710, 749, 1043, 1278, 1394
HpyF3I CTNAG 2 cut(s) 193, 904
HpySE526I ACGT 2 cut(s) 841, 1448
Hsp92I GRCGYC 5 cut(s) 64, 144, 1272, 1364, 1386
Hsp92II CATG 9 cut(s) 79, 397, 515, 747, 816, 896, 1103, 1146, 1460
HspAI GCGC 5 cut(s) 5, 7, 1272, 1364, 1460
KasI GGCGCC 2 cut(s) 1271, 1363
Kpn2I TCCGGA 1 cut(s) 1114
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Kzo9I GATC 7 cut(s) 631, 640, 922, 958, 974, 1000, 1294
LmnI GCTCC 6 cut(s) 718, 757, 1014, 1027, 1341, 1349
Lsp1109I GCAGC 7 cut(s) 50, 97, 262, 688, 716, 791, 1186
LweI GCATC 3 cut(s) 367, 730, 1211
MabI ACCWGGT 1 cut(s) 594
MaeII ACGT 2 cut(s) 841, 1448
MaeIII GTNAC 1 cut(s) 328
MalI GATC 7 cut(s) 633, 642, 924, 960, 976, 1002, 1296
MbiI CCGCTC 1 cut(s) 422
MboI GATC 7 cut(s) 631, 640, 922, 958, 974, 1000, 1294
MflI RGATCY 3 cut(s) 922, 958, 974
MhlI GDGCHC 2 cut(s) 774, 1346
MluCI AATT 6 cut(s) 486, 560, 572, 929, 1414, 1480
Mly113I GGCGCC 2 cut(s) 1272, 1364
MlyI GAGTC 1 cut(s) 1137
MmeI TCCRAC 1 cut(s) 1240
Mph1103I ATGCAT 1 cut(s) 745
MroI TCCGGA 1 cut(s) 1114
MroNI GCCGGC 1 cut(s) 23
MseI TTAA 9 cut(s) 71, 563, 837, 845, 882, 911, 1062, 1179, 1443
MslI CAYNNNNRTG 1 cut(s) 1248
MspI CCGG 7 cut(s) 24, 61, 100, 623, 943, 1115, 1377
MspR9I CCNGG 8 cut(s) 100, 596, 624, 723, 756, 944, 1026, 1339
MssI GTTTAAAC 1 cut(s) 72
Mva1269I GAATGC 2 cut(s) 277, 1283
MvaI CCWGG 5 cut(s) 596, 723, 756, 1026, 1339
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 6 cut(s) 13, 710, 749, 1043, 1278, 1394
NaeI GCCGGC 1 cut(s) 25
NarI GGCGCC 2 cut(s) 1272, 1364
NciI CCSGG 3 cut(s) 100, 624, 944
NdeII GATC 7 cut(s) 631, 640, 922, 958, 974, 1000, 1294
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 9 cut(s) 79, 397, 515, 747, 816, 896, 1103, 1146, 1460
NlaIV GGNNCC 8 cut(s) 166, 600, 753, 1036, 1244, 1273, 1365, 1375
NmeAIII GCCGAG 2 cut(s) 409, 728
NmuCI GTSAC 1 cut(s) 328
NsbI TGCGCA 1 cut(s) 1461
NsiI ATGCAT 1 cut(s) 745
NspI RCATGY 4 cut(s) 747, 816, 1146, 1460
OliI CACNNNNGTG 1 cut(s) 1248
PaeI GCATGC 1 cut(s) 747
PalAI GGCGCGCC 1 cut(s) 5
PauI GCGCGC 1 cut(s) 5
PcsI WCGNNNNNNNCGW 4 cut(s) 514, 1173, 1272, 1471
PctI GAATGC 2 cut(s) 277, 1283
PdiI GCCGGC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 613
PfoI TCCNGGA 3 cut(s) 98, 754, 1337
PleI GAGTC 1 cut(s) 1136
PluTI GGCGCC 2 cut(s) 1275, 1367
PmeI GTTTAAAC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 1136
Psp124BI GAGCTC 1 cut(s) 1346
Psp1406I AACGTT 1 cut(s) 841
Psp6I CCWGG 5 cut(s) 594, 721, 754, 1024, 1337
PspGI CCWGG 5 cut(s) 594, 721, 754, 1024, 1337
PspN4I GGNNCC 8 cut(s) 166, 600, 753, 1036, 1244, 1273, 1365, 1375
PspPI GGNCC 5 cut(s) 26, 156, 940, 945, 1290
PstI CTGCAG 3 cut(s) 40, 417, 706
PsuI RGATCY 3 cut(s) 922, 958, 974
PteI GCGCGC 1 cut(s) 5
RsaI GTAC 2 cut(s) 285, 1425
RsaNI GTAC 2 cut(s) 284, 1424
RseI CAYNNNNRTG 1 cut(s) 1248
Rsr2I CGGWCCG 1 cut(s) 156
RsrII CGGWCCG 1 cut(s) 156
SacI GAGCTC 1 cut(s) 1346
SaqAI TTAA 9 cut(s) 71, 563, 837, 845, 882, 911, 1062, 1179, 1443
Sau3AI GATC 7 cut(s) 631, 640, 922, 958, 974, 1000, 1294
Sau96I GGNCC 5 cut(s) 26, 156, 940, 945, 1290
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 1 cut(s) 1137
ScrFI CCNGG 8 cut(s) 100, 596, 624, 723, 756, 944, 1026, 1339
SduI GDGCHC 2 cut(s) 774, 1346
SexAI ACCWGGT 1 cut(s) 594
SfaNI GCATC 3 cut(s) 367, 730, 1211
SfcI CTRYAG 3 cut(s) 36, 413, 702
SfoI GGCGCC 2 cut(s) 1273, 1365
SgsI GGCGCGCC 1 cut(s) 5
SinI GGWCC 3 cut(s) 156, 940, 1290
SmiMI CAYNNNNRTG 1 cut(s) 1248
SphI GCATGC 1 cut(s) 747
Sse9I AATT 6 cut(s) 486, 560, 572, 929, 1414, 1480
SsiI CCGC 8 cut(s) 29, 32, 42, 154, 380, 420, 1074, 1246
SspDI GGCGCC 2 cut(s) 1271, 1363
SspI AATATT 1 cut(s) 880
SstI GAGCTC 1 cut(s) 1346
StyD4I CCNGG 8 cut(s) 98, 594, 622, 721, 754, 942, 1024, 1337
StyI CCWWGG 2 cut(s) 136, 936
TaaI ACNGT 3 cut(s) 188, 769, 1499
TaiI ACGT 2 cut(s) 844, 1451
TaqI TCGA 5 cut(s) 517, 558, 1161, 1266, 1400
TaqII GACCGA 2 cut(s) 338, 1307
TasI AATT 6 cut(s) 486, 560, 572, 929, 1414, 1480
TatI WGTACW 1 cut(s) 283
TauI GCSGC 3 cut(s) 34, 44, 1076
TfiI GAWTC 1 cut(s) 613
Tru1I TTAA 9 cut(s) 71, 563, 837, 845, 882, 911, 1062, 1179, 1443
Tru9I TTAA 9 cut(s) 71, 563, 837, 845, 882, 911, 1062, 1179, 1443
TscAI CASTG 2 cut(s) 553, 1098
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 7 cut(s) 38, 110, 275, 701, 704, 779, 1199
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 4 cut(s) 351, 498, 840, 1312
TspGWI ACGGA 2 cut(s) 148, 1480
TspRI CASTG 2 cut(s) 553, 1098
VneI GTGCAC 1 cut(s) 770
VpaK11BI GGWCC 3 cut(s) 156, 940, 1290
XagI CCTNNNNNAGG 2 cut(s) 196, 1402
XapI RAATTY 1 cut(s) 486
XceI RCATGY 4 cut(s) 747, 816, 1146, 1460
ZrmI AGTACT 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.