FvH4_2g32080

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010686
Physical Location & Seq
Reverse (-)
24411554 .. 24416287
4734 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32080.t1

Sequence Viewer

Length: 1053 bp
ATGGGGGATTCTAGTGGTGATTCAGTGTCGGTGGATATGGAGGGGGCTTCTCTCTGTGGGAAGGAGCATCTTGTACAAACTAGGCATGGTTATGTGTCCGTTGCTGTGTTGGGGGATCAGGACAAACCAGCTCTTGTCACTTATCCTGATTTAGCTTTAAATCATATGTCCTGCTTTCAAGGATTATTCTTTTGTCCAGAAGCATGTTCTTTGCTGCTCCACAACTTTTGCATATACCATATTAGTCCTCCCGGCCACGAGTTTGGAGCTTCAGCGATTAGCCTTGATGATCCTGCGTATTCCACTGATGATTTAGTAGATCAGATTGCCGAAGTTCTCAACTTTTTTGGACTTGGTGCAGTGATGTGCATGGGGGTTACAGCTGGAGCTTACATTCTTACTCATTTTGCTATGAAATACAGATCCCGTGTTATGGGGTTGATACTCGTTTCTCCAATATGCAAAGCACCATGCTGGACAGAATGGTTGTATAATAAGGTGATGTCAAATTTACTGTACTTCTATGGTATGTGTGGTGTGGTAAAGGAGATATTGCTTAAGCGCTACTTCAGCAAGGAAGTTCGTGGCAGTGTTCAAGTACCAGAATCAGATATAGTTCAGGCATGCAGAAGATCGCTTGATGAAAGGCGGAGTTCAAATGTTTGGCGGTTTCTTGAAGCAATCAATGGGAGACCCGACATTTCTGATGGATTGCGTAAGCTACAATGTCGCTCACTAATATTTGTTGGTGAGAACTCACCCTTTCACTCGGAAGCTCTCTACATGACATCAAAATTAGATAGGCGATATAGTGCCTTGGTTGAGGTTCAGGCATGTGGATCAATGGTGACAGAGGAGCAGCCTCATGCAATGTTGATACCATTGGAGTACTTCCTCATGGGTTATGGCTTATACAGGCCTTCTCAGTCAAATGTCAGCCCAAGAAGCCCCTTGAGTCCGTCCTGCATCGCACCAGAGCTTCTTTCACCAGAAAGCATGGGTTTGAAGTTGAAACCGATAAAGACTCGGGTACCTCATGATATAAGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010817 GO:0032879 GO:0050789 GO:0051049 GO:0065007 GO:0065008 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

351

Amino Acids

38.87

Weight (kDa)

6.07

Isoelectric Point (pI)

57.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndr PF03096 22 - 306 1.3e-108 Ndr family
Abhydrolase_1 PF00561 71 - 199 2.8e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11790 AT5G11790
fragaria_vesca FvH4_2g32080 FvH4_2g32080 FvH4_2g32080 FvH4_2g32080
malus_domestica MD08G1043700.v1.1 MD15G1061200.v1.1
prunus_persica Prupe.1G413900_v2.0.a1
pyrus_communis pycom08g03530 pycom15g05730
rosa_chinensis RchiOBHm_Chr6g0310661
rosa_laevigata RLG00000010468
rosa_multiflora Rmu_sc0007390.1_g000005
rosa_roxburghii Rroxscaffold_7G00158370
rosa_rugosa Rorug06G0382000
rosa_samantha Rh6AG496000 Rh6BG505500 Rh6CG511100
rosa_wichuraiana Rw6G043130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1030
AccB1I GGYRCC 1 cut(s) 1030
AciI CCGC 2 cut(s) 649, 667
AclWI GGATC 4 cut(s) 123, 284, 417, 847
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 1 cut(s) 508
AcuI CTGAAG 2 cut(s) 255, 553
AfaI GTAC 5 cut(s) 75, 518, 600, 890, 1032
AfeI AGCGCT 1 cut(s) 563
AfiI CCNNNNNNNGG 1 cut(s) 433
AflII CTTAAG 1 cut(s) 557
AgsI TTSAA 6 cut(s) 179, 596, 657, 677, 1006, 1012
AluBI AGCT 8 cut(s) 131, 155, 269, 383, 389, 721, 776, 979
AluI AGCT 8 cut(s) 131, 155, 269, 383, 389, 721, 776, 979
Alw26I GTCTC 1 cut(s) 685
AlwI GGATC 4 cut(s) 123, 284, 417, 847
Ama87I CYCGRG 1 cut(s) 1026
Aor51HI AGCGCT 1 cut(s) 563
AoxI GGCC 2 cut(s) 253, 917
ApeKI GCWGC 2 cut(s) 214, 859
ApoI RAATTY 1 cut(s) 508
Asp718I GGTACC 1 cut(s) 1030
AspLEI GCGC 1 cut(s) 564
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 6 cut(s) 29, 511, 750, 761, 859, 978
AvaI CYCGRG 1 cut(s) 1026
BaeI ACNNNNGTAYC 2 cut(s) 1014, 1047
BanI GGYRCC 1 cut(s) 1030
BauI CACGAG 1 cut(s) 257
BbvI GCAGC 2 cut(s) 201, 871
BccI CCATC 1 cut(s) 701
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 685
BfaI CTAG 2 cut(s) 12, 81
BfoI RGCGCY 1 cut(s) 565
BfrI CTTAAG 1 cut(s) 557
BisI GCNGC 2 cut(s) 215, 860
BlsI GCNGC 2 cut(s) 216, 861
BmcAI AGTACT 1 cut(s) 890
Bme1390I CCNGG 1 cut(s) 252
BmeT110I CYCGRG 1 cut(s) 1026
BmiI GGNNCC 1 cut(s) 1032
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 2 cut(s) 76, 975
BpmI CTGGAG 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 973
BpuMI CCSGG 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 685
BsaJI CCNNGG 1 cut(s) 816
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 876
BseDI CCNNGG 1 cut(s) 816
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMI GCAATG 1 cut(s) 876
BseMII CTCAG 1 cut(s) 938
BseRI GAGGAG 1 cut(s) 869
BseXI GCAGC 2 cut(s) 201, 871
BsgI GTGCAG 1 cut(s) 378
BshFI GGCC 2 cut(s) 255, 919
BshNI GGYRCC 1 cut(s) 1030
BsiHKCI CYCGRG 1 cut(s) 1026
BsiSI CCGG 1 cut(s) 252
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 685
BsnI GGCC 2 cut(s) 255, 919
Bso31I GGTCTC 1 cut(s) 685
BsoBI CYCGRG 1 cut(s) 1026
Bsp1407I TGTACA 1 cut(s) 73
Bsp143I GATC 6 cut(s) 115, 289, 319, 422, 632, 839
BspACI CCGC 2 cut(s) 649, 667
BspANI GGCC 2 cut(s) 255, 919
BspCNI CTCAG 1 cut(s) 937
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 1 cut(s) 1032
BspPI GGATC 4 cut(s) 123, 284, 417, 847
BspT107I GGYRCC 1 cut(s) 1030
BspTI CTTAAG 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 685
BsrDI GCAATG 1 cut(s) 876
BsrGI TGTACA 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 816
BssMI GATC 6 cut(s) 115, 289, 319, 422, 632, 839
BssSI CACGAG 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 816
Bst2BI CACGAG 1 cut(s) 257
Bst4CI ACNGT 1 cut(s) 516
BstAFI CTTAAG 1 cut(s) 557
BstAUI TGTACA 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 625
BstDEI CTNAG 1 cut(s) 924
BstH2I RGCGCY 1 cut(s) 565
BstHHI GCGC 1 cut(s) 564
BstKTI GATC 6 cut(s) 118, 292, 322, 425, 635, 842
BstMAI GTCTC 1 cut(s) 685
BstMBI GATC 6 cut(s) 115, 289, 319, 422, 632, 839
BstMWI GCNNNNNNNGC 2 cut(s) 570, 945
BstNSI RCATGY 3 cut(s) 207, 627, 837
BstSCI CCNGG 1 cut(s) 250
BstV1I GCAGC 2 cut(s) 201, 871
BstX2I RGATCY 1 cut(s) 422
BstXI CCANNNNNNTGG 1 cut(s) 263
BstYI RGATCY 1 cut(s) 422
BsuRI GGCC 2 cut(s) 255, 919
BtgZI GCGATG 1 cut(s) 952
BtsI GCAGTG 2 cut(s) 366, 595
BtsIMutI CAGTG 4 cut(s) 30, 303, 366, 595
Cac8I GCNNGC 1 cut(s) 625
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 1 cut(s) 564
Csp6I GTAC 5 cut(s) 74, 517, 599, 889, 1031
CspCI CAANNNNNGTGG 2 cut(s) 209, 244
CviQI GTAC 5 cut(s) 74, 517, 599, 889, 1031
DdeI CTNAG 1 cut(s) 924
DpnI GATC 6 cut(s) 117, 291, 321, 424, 634, 841
DpnII GATC 6 cut(s) 115, 289, 319, 422, 632, 839
DraI TTTAAA 1 cut(s) 159
EaeI YGGCCR 1 cut(s) 253
EciI GGCGGA 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 816
Eco147I AGGCCT 1 cut(s) 919
Eco31I GGTCTC 1 cut(s) 685
Eco47III AGCGCT 1 cut(s) 563
Eco57I CTGAAG 2 cut(s) 255, 553
Eco88I CYCGRG 1 cut(s) 1026
EcoT14I CCWWGG 1 cut(s) 816
ErhI CCWWGG 1 cut(s) 816
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FauNDI CATATG 1 cut(s) 165
Fnu4HI GCNGC 2 cut(s) 215, 860
Fsp4HI GCNGC 2 cut(s) 215, 860
FspBI CTAG 2 cut(s) 12, 81
GlaI GCGC 1 cut(s) 563
GluI GCNGC 2 cut(s) 215, 860
GsuI CTGGAG 1 cut(s) 405
HaeII RGCGCY 1 cut(s) 565
HaeIII GGCC 2 cut(s) 255, 919
HapII CCGG 1 cut(s) 252
HhaI GCGC 1 cut(s) 564
Hin6I GCGC 1 cut(s) 562
HinP1I GCGC 1 cut(s) 562
HinfI GANTC 5 cut(s) 8, 20, 605, 955, 1024
HpaII CCGG 1 cut(s) 252
HphI GGTGA 6 cut(s) 29, 511, 750, 761, 859, 978
Hpy188I TCNGA 4 cut(s) 324, 610, 706, 772
Hpy188III TCNNGA 5 cut(s) 119, 146, 197, 674, 1037
HpyAV CCTTC 2 cut(s) 55, 930
HpyCH4III ACNGT 1 cut(s) 516
HpyCH4V TGCA 7 cut(s) 231, 359, 369, 462, 627, 869, 966
HpyF10VI GCNNNNNNNGC 2 cut(s) 570, 945
HpyF3I CTNAG 1 cut(s) 924
HspAI GCGC 1 cut(s) 562
KpnI GGTACC 1 cut(s) 1034
Kzo9I GATC 6 cut(s) 115, 289, 319, 422, 632, 839
LmnI GCTCC 5 cut(s) 64, 222, 266, 386, 856
Lsp1109I GCAGC 2 cut(s) 201, 871
LweI GCATC 2 cut(s) 76, 975
MaeI CTAG 2 cut(s) 12, 81
MaeIII GTNAC 3 cut(s) 136, 376, 847
MalI GATC 6 cut(s) 117, 291, 321, 424, 634, 841
MboI GATC 6 cut(s) 115, 289, 319, 422, 632, 839
MboII GAAGA 1 cut(s) 642
MflI RGATCY 1 cut(s) 422
MluCI AATT 2 cut(s) 508, 794
MlyI GAGTC 2 cut(s) 964, 1018
MnlI CCTC 7 cut(s) 34, 258, 817, 847, 873, 905, 1044
MseI TTAA 2 cut(s) 158, 558
MslI CAYNNNNRTG 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 383
MspCI CTTAAG 1 cut(s) 557
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 252
MwoI GCNNNNNNNGC 2 cut(s) 570, 945
NciI CCSGG 1 cut(s) 252
NdeI CATATG 1 cut(s) 165
NdeII GATC 6 cut(s) 115, 289, 319, 422, 632, 839
NlaIV GGNNCC 1 cut(s) 1032
NmuCI GTSAC 2 cut(s) 136, 847
NspI RCATGY 3 cut(s) 207, 627, 837
PaeI GCATGC 1 cut(s) 627
PagI TCATGA 1 cut(s) 1036
PceI AGGCCT 1 cut(s) 919
PfeI GAWTC 3 cut(s) 8, 20, 605
PkrI GCNGC 2 cut(s) 216, 861
PleI GAGTC 2 cut(s) 963, 1018
PpsI GAGTC 2 cut(s) 963, 1018
PspN4I GGNNCC 1 cut(s) 1032
PsuI RGATCY 1 cut(s) 422
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 5 cut(s) 75, 518, 600, 890, 1032
RsaNI GTAC 5 cut(s) 74, 517, 599, 889, 1031
RseI CAYNNNNRTG 1 cut(s) 90
SaqAI TTAA 2 cut(s) 158, 558
SatI GCNGC 2 cut(s) 215, 860
Sau3AI GATC 6 cut(s) 115, 289, 319, 422, 632, 839
ScaI AGTACT 1 cut(s) 890
SchI GAGTC 2 cut(s) 964, 1018
ScrFI CCNGG 1 cut(s) 252
SfaNI GCATC 2 cut(s) 76, 975
SmiMI CAYNNNNRTG 1 cut(s) 90
SmlI CTYRAG 2 cut(s) 557, 952
SmoI CTYRAG 2 cut(s) 557, 952
SphI GCATGC 1 cut(s) 627
Sse9I AATT 2 cut(s) 508, 794
SseBI AGGCCT 1 cut(s) 919
SsiI CCGC 2 cut(s) 649, 667
SspI AATATT 1 cut(s) 741
SspMI CTAG 2 cut(s) 12, 81
StuI AGGCCT 1 cut(s) 919
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 816
TaaI ACNGT 1 cut(s) 516
TasI AATT 2 cut(s) 508, 794
TatI WGTACW 3 cut(s) 73, 516, 888
TfiI GAWTC 3 cut(s) 8, 20, 605
Tru1I TTAA 2 cut(s) 158, 558
Tru9I TTAA 2 cut(s) 158, 558
TscAI CASTG 4 cut(s) 30, 310, 366, 595
TseFI GTSAC 2 cut(s) 136, 847
TseI GCWGC 2 cut(s) 214, 859
Tsp45I GTSAC 2 cut(s) 136, 847
TspDTI ATGAA 2 cut(s) 428, 657
TspGWI ACGGA 2 cut(s) 88, 948
TspRI CASTG 4 cut(s) 30, 310, 366, 595
Vha464I CTTAAG 1 cut(s) 557
XapI RAATTY 1 cut(s) 508
XceI RCATGY 3 cut(s) 207, 627, 837
XspI CTAG 2 cut(s) 12, 81
ZrmI AGTACT 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.