MD15G1061200.v1.1

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010686
Physical Location & Seq
Reverse (-)
4108689 .. 4112373
3685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1061200.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGGGGATTCAAGTGATTCAGTGTCGGTGGATATGGAGGGGGTTTCTCTTGGCGGAAAGGAGCATATTGTGAGAACCGGTCTTGGTATTGTGTCGGTCGCTGTGTTGGGAGATCCGGACAAGCCAGCTCTTGTCACGTATCCTGATTTAGCCTTAAACCATATGTCCTGCTTTCAAGGATTATTCTTTTGTCCAGAAGCTTGTTCCTTGCTGCTTCACAACTTTTGCATATATCATATCAGTCCTCCTGGCCATGAGTTGGGAGCTTCTGCAATTAGCCTTGATGATCCGTTGTATTCTGTTGATGATTTAGTGGATCAGATTGCCGAGGTTCTCGACTTTTTTGGACTCGGTGCAGTGATGTGTATGGGGGTTACAGCTGGAGCTTTCATTCTCACCCAATTTGCTATGAAATATAGACCACGTGTCATGGGTTTGATACTTGTTTCACCTATATGCAAAGCAGCCACTTGGACAGAATGGTTGTATAATAAGGTGATGTCGAATTTACTCTACTTTTATGGCATGTGCGGCGTGGTTAAGGAGATATTGCTTAAGCGGTACTTCAGCAAGGAAGTTCGTGGTAGTGTTCAAGTACCAGAGTCAGATATAGTTCAGGCATGCAGAAGATCACTTGATGAAAGGCATAGTTCGAATGTTTGGCGATTTCTTGAGGCAATGAATGGGAGACCTGACATTTCTGAAGGATTGAGAAAGCTACAATGCCGTTCGCTAATATTTGTTGGTGACAGCTCTCCGTTTCACTCAGAAGCTCTCTACATGACTTCAAAACTAGATAGAAGATATAGTGCCTTGGTTGAGGTTCAGGCATGTGGGTCAATGGTGACAGAAGAGCAGCCGCATGCGATGTTGATACCGCTAGAGTACTTCCTCATGGGATATGGTTTGTACAGGCCTTCTCAGTCCAGTGTCAGCCCAAGAAGCCCCTTGAGTCCGTCCTGCATCGCACCTGAGCTTCTTTCACCAGAAAGCATGGGCTTGAAGTTGAAACCGATAAAAACTCGAATTCCTCATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010817 GO:0032879 GO:0050789 GO:0051049 GO:0065007 GO:0065008 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

347

Amino Acids

38.21

Weight (kDa)

5.86

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndr PF03096 21 - 305 3.1e-108 Ndr family
Abhydrolase_1 PF00561 70 - 198 1.1e-08 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11790 AT5G11790
fragaria_vesca FvH4_2g32080 FvH4_2g32080 FvH4_2g32080 FvH4_2g32080
malus_domestica MD08G1043700.v1.1 MD15G1061200.v1.1
prunus_persica Prupe.1G413900_v2.0.a1
pyrus_communis pycom08g03530 pycom15g05730
rosa_chinensis RchiOBHm_Chr6g0310661
rosa_laevigata RLG00000010468
rosa_multiflora Rmu_sc0007390.1_g000005
rosa_roxburghii Rroxscaffold_7G00158370
rosa_rugosa Rorug06G0382000
rosa_samantha Rh6AG496000 Rh6BG505500 Rh6CG511100
rosa_wichuraiana Rw6G043130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 259
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 5 cut(s) 54, 531, 559, 860, 878
AclWI GGATC 3 cut(s) 107, 281, 324
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 2 cut(s) 505, 1026
AcuI CTGAAG 2 cut(s) 550, 723
AcvI CACGTG 1 cut(s) 425
AfaI GTAC 4 cut(s) 563, 597, 887, 911
AfiI CCNNNNNNNGG 1 cut(s) 259
AflII CTTAAG 1 cut(s) 554
AflIII ACRYGT 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 77
AgsI TTSAA 6 cut(s) 12, 176, 593, 789, 1003, 1009
AhdI GACNNNNNGTC 1 cut(s) 425
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 9 cut(s) 128, 200, 266, 380, 386, 718, 753, 773, 976
AluI AGCT 9 cut(s) 128, 200, 266, 380, 386, 718, 753, 773, 976
Alw26I GTCTC 1 cut(s) 682
AlwI GGATC 3 cut(s) 107, 281, 324
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 2 cut(s) 250, 914
ApeKI GCWGC 3 cut(s) 211, 464, 856
ApoI RAATTY 2 cut(s) 505, 1026
AsiGI ACCGGT 1 cut(s) 77
AsuHPI GGTGA 6 cut(s) 388, 441, 508, 758, 856, 975
AsuII TTCGAA 1 cut(s) 653
BalI TGGCCA 1 cut(s) 252
BbrPI CACGTG 1 cut(s) 425
BbvI GCAGC 3 cut(s) 198, 476, 868
BceAI ACGGC 1 cut(s) 711
BciT130I CCWGG 1 cut(s) 249
BciVI GTATCC 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 682
BfaI CTAG 2 cut(s) 794, 881
BfrI CTTAAG 1 cut(s) 554
BfuI GTATCC 1 cut(s) 150
BisI GCNGC 5 cut(s) 212, 465, 532, 857, 860
BlsI GCNGC 5 cut(s) 213, 466, 533, 858, 861
BmcAI AGTACT 1 cut(s) 887
Bme1390I CCNGG 1 cut(s) 249
BmeRI GACNNNNNGTC 1 cut(s) 425
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 972
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 972
Bpu14I TTCGAA 1 cut(s) 653
BpuEI CTTGAG 2 cut(s) 692, 970
BsaAI YACGTR 2 cut(s) 138, 425
BsaI GGTCTC 1 cut(s) 682
BsaJI CCNNGG 2 cut(s) 327, 813
BsaWI WCCGGW 2 cut(s) 77, 115
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 927
Bse3DI GCAATG 1 cut(s) 684
BseAI TCCGGA 1 cut(s) 115
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 327, 813
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 3 cut(s) 780, 935, 963
BseNI ACTGG 1 cut(s) 927
BseXI GCAGC 3 cut(s) 198, 476, 868
BsgI GTGCAG 1 cut(s) 375
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 252, 916
BshTI ACCGGT 1 cut(s) 77
BsiEI CGRYCG 1 cut(s) 99
BsiSI CCGG 2 cut(s) 78, 116
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 1 cut(s) 682
BsnI GGCC 2 cut(s) 252, 916
Bso31I GGTCTC 1 cut(s) 682
Bsp119I TTCGAA 1 cut(s) 653
Bsp13I TCCGGA 1 cut(s) 115
Bsp1407I TGTACA 1 cut(s) 909
Bsp143I GATC 4 cut(s) 112, 286, 316, 629
BspACI CCGC 5 cut(s) 54, 531, 559, 860, 878
BspANI GGCC 2 cut(s) 252, 916
BspCNI CTCAG 3 cut(s) 779, 934, 964
BspEI TCCGGA 1 cut(s) 115
BspPI GGATC 3 cut(s) 107, 281, 324
BspQI GCTCTTC 1 cut(s) 846
BspT104I TTCGAA 1 cut(s) 653
BspTI CTTAAG 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 682
BsrDI GCAATG 1 cut(s) 684
BsrFI RCCGGY 1 cut(s) 77
BsrGI TGTACA 1 cut(s) 909
BsrI ACTGG 1 cut(s) 927
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 2 cut(s) 327, 813
BssMI GATC 4 cut(s) 112, 286, 316, 629
BssT1I CCWWGG 1 cut(s) 813
Bst2UI CCWGG 1 cut(s) 249
Bst6I CTCTTC 1 cut(s) 846
BstAFI CTTAAG 1 cut(s) 554
BstAUI TGTACA 1 cut(s) 909
BstBAI YACGTR 2 cut(s) 138, 425
BstBI TTCGAA 1 cut(s) 653
BstC8I GCNNGC 3 cut(s) 126, 622, 864
BstDEI CTNAG 3 cut(s) 766, 921, 972
BstKTI GATC 4 cut(s) 115, 289, 319, 632
BstMAI GTCTC 1 cut(s) 682
BstMBI GATC 4 cut(s) 112, 286, 316, 629
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 2 cut(s) 531, 942
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 4 cut(s) 529, 624, 834, 866
BstSCI CCNGG 1 cut(s) 247
BstV1I GCAGC 3 cut(s) 198, 476, 868
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
BsuI GTATCC 1 cut(s) 150
BsuRI GGCC 2 cut(s) 252, 916
BtgZI GCGATG 2 cut(s) 881, 949
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 27, 363, 934
Cac8I GCNNGC 3 cut(s) 126, 622, 864
Cfr10I RCCGGY 1 cut(s) 77
Csp6I GTAC 4 cut(s) 562, 596, 886, 910
CspAI ACCGGT 1 cut(s) 77
CviAII CATG 9 cut(s) 254, 430, 526, 621, 781, 831, 863, 895, 994
CviQI GTAC 4 cut(s) 562, 596, 886, 910
DdeI CTNAG 3 cut(s) 766, 921, 972
DpnI GATC 4 cut(s) 114, 288, 318, 631
DpnII GATC 4 cut(s) 112, 286, 316, 629
DriI GACNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 846
Eam1105I GACNNNNNGTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 846
EciI GGCGGA 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 813
Eco147I AGGCCT 1 cut(s) 916
Eco31I GGTCTC 1 cut(s) 682
Eco57I CTGAAG 2 cut(s) 550, 723
Eco72I CACGTG 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 1026
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 813
ErhI CCWWGG 1 cut(s) 813
FaeI CATG 9 cut(s) 257, 433, 529, 624, 784, 834, 866, 898, 997
FalI AAGNNNNNCTT 2 cut(s) 548, 580
FatI CATG 9 cut(s) 253, 429, 525, 620, 780, 830, 862, 894, 993
FauNDI CATATG 1 cut(s) 162
Fnu4HI GCNGC 5 cut(s) 212, 465, 532, 857, 860
Fsp4HI GCNGC 5 cut(s) 212, 465, 532, 857, 860
FspBI CTAG 2 cut(s) 794, 881
GluI GCNGC 5 cut(s) 212, 465, 532, 857, 860
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 2 cut(s) 252, 916
HapII CCGG 2 cut(s) 78, 116
Hin1II CATG 9 cut(s) 257, 433, 529, 624, 784, 834, 866, 898, 997
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 5 cut(s) 8, 17, 348, 602, 952
HpaII CCGG 2 cut(s) 78, 116
HphI GGTGA 6 cut(s) 388, 441, 508, 758, 856, 975
Hpy188I TCNGA 4 cut(s) 321, 607, 703, 769
Hpy188III TCNNGA 6 cut(s) 116, 143, 194, 335, 671, 1038
HpyAV CCTTC 2 cut(s) 698, 927
HpyCH4IV ACGT 2 cut(s) 137, 424
HpyCH4V TGCA 6 cut(s) 228, 272, 356, 459, 624, 963
HpyF10VI GCNNNNNNNGC 2 cut(s) 531, 942
HpyF3I CTNAG 3 cut(s) 766, 921, 972
HpySE526I ACGT 2 cut(s) 137, 424
Hsp92II CATG 9 cut(s) 257, 433, 529, 624, 784, 834, 866, 898, 997
Kpn2I TCCGGA 1 cut(s) 115
Kzo9I GATC 4 cut(s) 112, 286, 316, 629
LguI GCTCTTC 1 cut(s) 846
LmnI GCTCC 3 cut(s) 61, 263, 383
Lsp1109I GCAGC 3 cut(s) 198, 476, 868
LweI GCATC 1 cut(s) 972
MaeI CTAG 2 cut(s) 794, 881
MaeII ACGT 2 cut(s) 137, 424
MaeIII GTNAC 4 cut(s) 133, 373, 746, 844
MalI GATC 4 cut(s) 114, 288, 318, 631
MboI GATC 4 cut(s) 112, 286, 316, 629
MboII GAAGA 3 cut(s) 639, 813, 863
MflI RGATCY 1 cut(s) 112
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 4 cut(s) 273, 401, 505, 1026
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 3 cut(s) 342, 611, 961
MnlI CCTC 7 cut(s) 31, 255, 322, 667, 814, 902, 1041
Mox20I TGGCCA 1 cut(s) 252
MroI TCCGGA 1 cut(s) 115
MscI TGGCCA 1 cut(s) 252
MseI TTAA 3 cut(s) 155, 540, 555
MslI CAYNNNNRTG 1 cut(s) 454
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 380
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 2 cut(s) 78, 116
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 531, 942
NdeI CATATG 1 cut(s) 162
NdeII GATC 4 cut(s) 112, 286, 316, 629
NlaIII CATG 9 cut(s) 257, 433, 529, 624, 784, 834, 866, 898, 997
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 3 cut(s) 133, 746, 844
NspI RCATGY 4 cut(s) 529, 624, 834, 866
NspV TTCGAA 1 cut(s) 653
PaeI GCATGC 2 cut(s) 624, 866
PceI AGGCCT 1 cut(s) 916
PciSI GCTCTTC 1 cut(s) 846
PfeI GAWTC 2 cut(s) 8, 17
PflMI CCANNNNNTGG 1 cut(s) 259
PinAI ACCGGT 1 cut(s) 77
PkrI GCNGC 5 cut(s) 213, 466, 533, 858, 861
PleI GAGTC 3 cut(s) 342, 610, 960
PmaCI CACGTG 1 cut(s) 425
PmlI CACGTG 1 cut(s) 425
PpsI GAGTC 3 cut(s) 342, 610, 960
Ppu21I YACGTR 2 cut(s) 138, 425
Psp6I CCWGG 1 cut(s) 247
PspCI CACGTG 1 cut(s) 425
PspGI CCWGG 1 cut(s) 247
PsuI RGATCY 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 4 cut(s) 563, 597, 887, 911
RsaNI GTAC 4 cut(s) 562, 596, 886, 910
RseI CAYNNNNRTG 1 cut(s) 454
SapI GCTCTTC 1 cut(s) 846
SaqAI TTAA 3 cut(s) 155, 540, 555
SatI GCNGC 5 cut(s) 212, 465, 532, 857, 860
Sau3AI GATC 4 cut(s) 112, 286, 316, 629
ScaI AGTACT 1 cut(s) 887
SchI GAGTC 3 cut(s) 342, 611, 961
ScrFI CCNGG 1 cut(s) 249
SfaNI GCATC 1 cut(s) 972
SfuI TTCGAA 1 cut(s) 653
SmiMI CAYNNNNRTG 1 cut(s) 454
SmlI CTYRAG 3 cut(s) 554, 671, 949
SmoI CTYRAG 3 cut(s) 554, 671, 949
SphI GCATGC 2 cut(s) 624, 866
Sse9I AATT 4 cut(s) 273, 401, 505, 1026
SseBI AGGCCT 1 cut(s) 916
SsiI CCGC 5 cut(s) 54, 531, 559, 860, 878
SspI AATATT 1 cut(s) 738
SspMI CTAG 2 cut(s) 794, 881
StuI AGGCCT 1 cut(s) 916
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 1 cut(s) 813
TaiI ACGT 2 cut(s) 140, 427
TaqI TCGA 4 cut(s) 336, 503, 653, 1024
TaqII GACCGA 1 cut(s) 85
TasI AATT 4 cut(s) 273, 401, 505, 1026
TatI WGTACW 2 cut(s) 885, 909
TauI GCSGC 2 cut(s) 534, 862
TfiI GAWTC 2 cut(s) 8, 17
Tru1I TTAA 3 cut(s) 155, 540, 555
Tru9I TTAA 3 cut(s) 155, 540, 555
TscAI CASTG 3 cut(s) 27, 363, 934
TseFI GTSAC 3 cut(s) 133, 746, 844
TseI GCWGC 3 cut(s) 211, 464, 856
Tsp45I GTSAC 3 cut(s) 133, 746, 844
TspDTI ATGAA 4 cut(s) 379, 425, 654, 695
TspGWI ACGGA 3 cut(s) 279, 747, 945
TspRI CASTG 3 cut(s) 27, 363, 934
Van91I CCANNNNNTGG 1 cut(s) 259
Vha464I CTTAAG 1 cut(s) 554
XapI RAATTY 2 cut(s) 505, 1026
XceI RCATGY 4 cut(s) 529, 624, 834, 866
XspI CTAG 2 cut(s) 794, 881
ZrmI AGTACT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.