MD08G1043700.v1.1

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010686
Physical Location & Seq
Reverse (-)
3304206 .. 3307887
3682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1043700.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGGGGATTCAAGTGATTCAGTGTCGGTGGATATGGAGGGGGTTTCTCTTGGCGGAAAGGAGCATCTTGTAAGAACCTGTCTTGGTTATGTGTCCGTCGCTGTGTTGGGAGATCCGGACAAGCCAGCTCTTGTCACGTATCCTGATTTAGCCTTAAATCATATGTCCTGCTTTCAAGGATTATTCCTTTGTCCAGAAGCTTGTTCCTTGCTGCTCCACAACTTTTGCATATATCATATCAGTCCTCCTGGGCATGAGTTGGGAGCTTCTGCAATCAGCCTTGATGATCCTTTGTATTCTGTTGATGATTTAGTAGATCAGATTGCTGAGGTTCTCGATTTTTTCGGACTCGGTGCAGTAATGTGTATGGGGGTTACAGCTGGAGCTTTCATTCTCACCCAATTTGCTATGAAATATAGACCACGTGTCATGGGTTTGATACTTGTTTCTCCTATATCCAAAGCAGCCACTTGGACAGAATGGTTGTATAATAAGGTGATGTTGAATTTACTCTACTTTTATGGCATGTGTGGGGTGGTAAAGGAGATATTGCTTAACCGGTACTTCAGCAAGGAAGTTCGTGGTGGTGTTCAAGTACCAGAGTCAGATATAGTTCAGGCATGCAGAAGATCGCTCGATGAAAGGCATAGTTCAAATGTTTGGCGGTTTCTTGAAGCAATGAATGGGAGGCCTGACATTTCTGAAGGATTGAGAAAGCTACAATGCCGTACGCTAATATTTGTTGGGGACAGCTCTCCGTTTCACTCAGAAGCTCTGTACATGACCTCAAAACTAGATAGAAGATATAGTGCCTTGGTTGAGGTTCAGGCATGCGGGTCAATGGTGACAGAGGAGCAGCCACATGCAATGTTGATACCGCTAGAGTACTTCCTCATGGGATATGGTTTATACAGGCCTTCTCAGTCTAGTGTCAGCCCAAGAAGCCCCTTGAGTCCTTCCTGCATCGCACCTGAGCTTCTTTCCCCAGAAAGCATGGGCTTGAAGTTGAAACCGATAAAAACTCGGATTCCTCATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010817 GO:0032879 GO:0050789 GO:0051049 GO:0065007 GO:0065008 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

347

Amino Acids

38.25

Weight (kDa)

5.73

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndr PF03096 21 - 305 1e-106 Ndr family
Abhydrolase_1 PF00561 73 - 197 1.3e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11790 AT5G11790
fragaria_vesca FvH4_2g32080 FvH4_2g32080 FvH4_2g32080 FvH4_2g32080
malus_domestica MD08G1043700.v1.1 MD15G1061200.v1.1
prunus_persica Prupe.1G413900_v2.0.a1
pyrus_communis pycom08g03530 pycom15g05730
rosa_chinensis RchiOBHm_Chr6g0310661
rosa_laevigata RLG00000010468
rosa_multiflora Rmu_sc0007390.1_g000005
rosa_roxburghii Rroxscaffold_7G00158370
rosa_rugosa Rorug06G0382000
rosa_samantha Rh6AG496000 Rh6BG505500 Rh6CG511100
rosa_wichuraiana Rw6G043130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 4 cut(s) 54, 664, 834, 878
AclWI GGATC 2 cut(s) 107, 281
AcsI RAATTY 1 cut(s) 505
AcuI CTGAAG 2 cut(s) 550, 723
AcvI CACGTG 1 cut(s) 425
AfaI GTAC 5 cut(s) 563, 597, 730, 779, 887
AflIII ACRYGT 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 558
AgsI TTSAA 8 cut(s) 12, 176, 505, 593, 654, 674, 1003, 1009
AhdI GACNNNNNGTC 1 cut(s) 425
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 9 cut(s) 128, 200, 266, 380, 386, 718, 753, 773, 976
AluI AGCT 9 cut(s) 128, 200, 266, 380, 386, 718, 753, 773, 976
AlwI GGATC 2 cut(s) 107, 281
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 2 cut(s) 689, 914
ApeKI GCWGC 3 cut(s) 211, 464, 856
ApoI RAATTY 1 cut(s) 505
AsiGI ACCGGT 1 cut(s) 558
AsuHPI GGTGA 3 cut(s) 388, 508, 856
BbrPI CACGTG 1 cut(s) 425
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 3 cut(s) 198, 476, 868
BceAI ACGGC 1 cut(s) 711
BciT130I CCWGG 1 cut(s) 249
BciVI GTATCC 1 cut(s) 150
BfaI CTAG 3 cut(s) 794, 881, 927
BfuI GTATCC 1 cut(s) 150
BisI GCNGC 3 cut(s) 212, 465, 857
BlsI GCNGC 3 cut(s) 213, 466, 858
BmcAI AGTACT 1 cut(s) 887
Bme1390I CCNGG 1 cut(s) 249
BmeRI GACNNNNNGTC 1 cut(s) 425
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 73, 972
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 2 cut(s) 327, 972
BpuEI CTTGAG 1 cut(s) 970
BsaAI YACGTR 2 cut(s) 138, 425
BsaJI CCNNGG 2 cut(s) 248, 813
BsaWI WCCGGW 2 cut(s) 115, 558
Bse118I RCCGGY 1 cut(s) 558
Bse3DI GCAATG 2 cut(s) 684, 873
BseAI TCCGGA 1 cut(s) 115
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 248, 813
BseMI GCAATG 2 cut(s) 684, 873
BseMII CTCAG 4 cut(s) 318, 780, 935, 963
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 3 cut(s) 198, 476, 868
BsgI GTGCAG 1 cut(s) 375
BshFI GGCC 2 cut(s) 691, 916
BshTI ACCGGT 1 cut(s) 558
BsiSI CCGG 2 cut(s) 116, 559
BsiWI CGTACG 1 cut(s) 728
BslFI GGGAC 1 cut(s) 761
BsmFI GGGAC 1 cut(s) 761
BsnI GGCC 2 cut(s) 691, 916
Bsp13I TCCGGA 1 cut(s) 115
Bsp1407I TGTACA 1 cut(s) 777
Bsp143I GATC 4 cut(s) 112, 286, 316, 629
BspACI CCGC 4 cut(s) 54, 664, 834, 878
BspANI GGCC 2 cut(s) 691, 916
BspCNI CTCAG 4 cut(s) 319, 779, 934, 964
BspEI TCCGGA 1 cut(s) 115
BspPI GGATC 2 cut(s) 107, 281
BsrDI GCAATG 2 cut(s) 684, 873
BsrFI RCCGGY 1 cut(s) 558
BsrGI TGTACA 1 cut(s) 777
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 2 cut(s) 248, 813
BssMI GATC 4 cut(s) 112, 286, 316, 629
BssT1I CCWWGG 1 cut(s) 813
Bst2UI CCWGG 1 cut(s) 249
BstAUI TGTACA 1 cut(s) 777
BstBAI YACGTR 2 cut(s) 138, 425
BstC8I GCNNGC 3 cut(s) 126, 622, 832
BstDEI CTNAG 4 cut(s) 327, 766, 921, 972
BstKTI GATC 4 cut(s) 115, 289, 319, 632
BstMBI GATC 4 cut(s) 112, 286, 316, 629
BstMWI GCNNNNNNNGC 1 cut(s) 942
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 4 cut(s) 529, 624, 834, 866
BstSCI CCNGG 1 cut(s) 247
BstV1I GCAGC 3 cut(s) 198, 476, 868
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
BsuI GTATCC 1 cut(s) 150
BsuRI GGCC 2 cut(s) 691, 916
BtgZI GCGATG 1 cut(s) 949
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 3 cut(s) 126, 622, 832
Cfr10I RCCGGY 1 cut(s) 558
Csp6I GTAC 5 cut(s) 562, 596, 729, 778, 886
CspAI ACCGGT 1 cut(s) 558
CspCI CAANNNNNGTGG 2 cut(s) 206, 241
CviAII CATG 9 cut(s) 254, 430, 526, 621, 781, 831, 863, 895, 994
CviQI GTAC 5 cut(s) 562, 596, 729, 778, 886
DdeI CTNAG 4 cut(s) 327, 766, 921, 972
DpnI GATC 4 cut(s) 114, 288, 318, 631
DpnII GATC 4 cut(s) 112, 286, 316, 629
DriI GACNNNNNGTC 1 cut(s) 425
Eam1105I GACNNNNNGTC 1 cut(s) 425
EciI GGCGGA 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 813
Eco147I AGGCCT 2 cut(s) 691, 916
Eco57I CTGAAG 2 cut(s) 550, 723
Eco72I CACGTG 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 813
ErhI CCWWGG 1 cut(s) 813
FaeI CATG 9 cut(s) 257, 433, 529, 624, 784, 834, 866, 898, 997
FaqI GGGAC 1 cut(s) 761
FatI CATG 9 cut(s) 253, 429, 525, 620, 780, 830, 862, 894, 993
FauI CCCGC 1 cut(s) 827
FauNDI CATATG 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 212, 465, 857
Fsp4HI GCNGC 3 cut(s) 212, 465, 857
FspBI CTAG 3 cut(s) 794, 881, 927
GluI GCNGC 3 cut(s) 212, 465, 857
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 2 cut(s) 691, 916
HapII CCGG 2 cut(s) 116, 559
Hin1II CATG 9 cut(s) 257, 433, 529, 624, 784, 834, 866, 898, 997
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 6 cut(s) 8, 17, 348, 602, 952, 1027
HpaII CCGG 2 cut(s) 116, 559
HphI GGTGA 3 cut(s) 388, 508, 856
Hpy188I TCNGA 6 cut(s) 321, 347, 607, 703, 769, 1026
Hpy188III TCNNGA 6 cut(s) 116, 143, 194, 335, 671, 1038
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 3 cut(s) 698, 927, 966
HpyCH4IV ACGT 2 cut(s) 137, 424
HpyCH4V TGCA 6 cut(s) 228, 272, 356, 624, 866, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 942
HpyF3I CTNAG 4 cut(s) 327, 766, 921, 972
HpySE526I ACGT 2 cut(s) 137, 424
Hsp92II CATG 9 cut(s) 257, 433, 529, 624, 784, 834, 866, 898, 997
Kpn2I TCCGGA 1 cut(s) 115
Kzo9I GATC 4 cut(s) 112, 286, 316, 629
LmnI GCTCC 5 cut(s) 61, 219, 263, 383, 853
Lsp1109I GCAGC 3 cut(s) 198, 476, 868
LweI GCATC 2 cut(s) 73, 972
MaeI CTAG 3 cut(s) 794, 881, 927
MaeII ACGT 2 cut(s) 137, 424
MaeIII GTNAC 3 cut(s) 133, 373, 844
MalI GATC 4 cut(s) 114, 288, 318, 631
MboI GATC 4 cut(s) 112, 286, 316, 629
MboII GAAGA 2 cut(s) 639, 813
MflI RGATCY 1 cut(s) 112
MluCI AATT 2 cut(s) 401, 505
MlyI GAGTC 3 cut(s) 342, 611, 961
MnlI CCTC 9 cut(s) 31, 255, 322, 681, 796, 814, 844, 902, 1041
MroI TCCGGA 1 cut(s) 115
MseI TTAA 2 cut(s) 155, 555
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 2 cut(s) 116, 559
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 942
NdeI CATATG 1 cut(s) 162
NdeII GATC 4 cut(s) 112, 286, 316, 629
NlaIII CATG 9 cut(s) 257, 433, 529, 624, 784, 834, 866, 898, 997
NmuCI GTSAC 2 cut(s) 133, 844
NspI RCATGY 4 cut(s) 529, 624, 834, 866
PaeI GCATGC 2 cut(s) 624, 834
PceI AGGCCT 2 cut(s) 691, 916
PfeI GAWTC 3 cut(s) 8, 17, 1027
Pfl23II CGTACG 1 cut(s) 728
PinAI ACCGGT 1 cut(s) 558
PkrI GCNGC 3 cut(s) 213, 466, 858
PleI GAGTC 3 cut(s) 342, 610, 960
PmaCI CACGTG 1 cut(s) 425
PmlI CACGTG 1 cut(s) 425
PpsI GAGTC 3 cut(s) 342, 610, 960
Ppu21I YACGTR 2 cut(s) 138, 425
Psp6I CCWGG 1 cut(s) 247
PspCI CACGTG 1 cut(s) 425
PspGI CCWGG 1 cut(s) 247
PspLI CGTACG 1 cut(s) 728
PsuI RGATCY 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 5 cut(s) 563, 597, 730, 779, 887
RsaNI GTAC 5 cut(s) 562, 596, 729, 778, 886
SaqAI TTAA 2 cut(s) 155, 555
SatI GCNGC 3 cut(s) 212, 465, 857
Sau3AI GATC 4 cut(s) 112, 286, 316, 629
ScaI AGTACT 1 cut(s) 887
SchI GAGTC 3 cut(s) 342, 611, 961
ScrFI CCNGG 1 cut(s) 249
SfaNI GCATC 2 cut(s) 73, 972
SmlI CTYRAG 1 cut(s) 949
SmoI CTYRAG 1 cut(s) 949
SphI GCATGC 2 cut(s) 624, 834
Sse9I AATT 2 cut(s) 401, 505
SseBI AGGCCT 2 cut(s) 691, 916
SsiI CCGC 4 cut(s) 54, 664, 834, 878
SspI AATATT 1 cut(s) 738
SspMI CTAG 3 cut(s) 794, 881, 927
StuI AGGCCT 2 cut(s) 691, 916
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 1 cut(s) 813
TaiI ACGT 2 cut(s) 140, 427
TaqI TCGA 2 cut(s) 336, 636
TasI AATT 2 cut(s) 401, 505
TatI WGTACW 2 cut(s) 777, 885
TfiI GAWTC 3 cut(s) 8, 17, 1027
Tru1I TTAA 2 cut(s) 155, 555
Tru9I TTAA 2 cut(s) 155, 555
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 2 cut(s) 133, 844
TseI GCWGC 3 cut(s) 211, 464, 856
Tsp45I GTSAC 2 cut(s) 133, 844
TspDTI ATGAA 4 cut(s) 379, 425, 654, 695
TspGWI ACGGA 2 cut(s) 85, 747
TspRI CASTG 1 cut(s) 27
XapI RAATTY 1 cut(s) 505
XceI RCATGY 4 cut(s) 529, 624, 834, 866
XspI CTAG 3 cut(s) 794, 881, 927
ZrmI AGTACT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.