FvH4_2g32140

Eukaryotic protein of unknown function (DUF1764)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013908
Physical Location & Seq
Reverse (-)
24444823 .. 24445549
727 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32140.t1

Sequence Viewer

Length: 663 bp
ATGGATCCAACTTACACTGGACTCCCTGATTTCATGAACGTCATTGATTTCGAGCCCATGTTACCCAATTCATCTCAATTCCCAGAAATCTCAAATCAGTACCAATTTAACCAGCTCTCTCCAGATCTTGATTTTCTGAGCAATCAGTTTTCAAATCCACCGGAACTGGGATCCGGTAATCTTGTTCCTCCAATTAGTGTTAGTAATGGAGGCTCATTTTCTGGTCCTACTACTATTTCTGATCCCCATAAGATGGGCAAGAAGAGTAAATCAAAGACCCCTCAAGAGGTTAAAGAAAAAAGTGTTGTGGAGAAGCCTGTTGTGGAAGAAGAGAAGCCTGCATCTTCTGCAAAAAAAGCTACTCCAGCAAAGAAGCCTGCTTCTGTGATTGATGAGATATTTGCTGGGAAAAAGAGGAAGAAAGCCGAAGCTGAAAAGGCTAAAAAGCAAAGTGAAGAGGCGAGTGAAAAACCCAACAAGCCTAAGAGAAAGAAGAATAAAGATTTAGGCTTTGTAAATGGTGCGTTTGTGGATGAAACTTCTGGGCCGAAAAAGAGGACAAACGATGGTCTTGCTATCTACACAGAGGAAGAGTTGGGTATCAACAAAGCAGATGCTGGAAGCACCCCACTTTGTCCATTTGATTGTGATTGCTGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1764

Protein Analysis

221

Amino Acids

23.96

Weight (kDa)

6.64

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1764 PF08576 123 - 220 1.1e-26 Eukaryotic protein of unknown function (DUF1764)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013908)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11760
fragaria_vesca FvH4_2g32140
malus_domestica MD08G1044600.v1.1 MD15G1061800.v1.1
prunus_persica Prupe.1G414500_v2.0.a1 Prupe.1G484700_v2.0.a1
pyrus_communis pycom08g03590
rosa_chinensis RchiOBHm_Chr6g0310541
rosa_multiflora Rmu_sc0005386.1_g000001 Rmu_sc0016392.1_g000003 Rmu_sc0020729.1_g000001
rosa_roxburghii Rroxscaffold_7G00158440
rosa_rugosa Rorug06G0381400 Rorug06G0381500
rosa_samantha Rh6AG495300 Rh6CG510300
rosa_wichuraiana Rw6G043080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 253
AclWI GGATC 4 cut(s) 12, 165, 178, 236
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 3 cut(s) 167, 253, 286
AgsI TTSAA 1 cut(s) 153
AluBI AGCT 3 cut(s) 115, 359, 431
AluI AGCT 3 cut(s) 115, 359, 431
AlwI GGATC 4 cut(s) 12, 165, 178, 236
AlwNI CAGNNNCTG 1 cut(s) 617
AoxI GGCC 1 cut(s) 545
ApeKI GCWGC 1 cut(s) 654
AspS9I GGNCC 2 cut(s) 224, 545
AvaII GGWCC 1 cut(s) 224
BamHI GGATCC 2 cut(s) 4, 170
BanII GRGCYC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 641
BccI CCATC 2 cut(s) 247, 560
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BglII AGATCT 1 cut(s) 124
BisI GCNGC 1 cut(s) 655
BlsI GCNGC 1 cut(s) 656
Bme18I GGWCC 1 cut(s) 224
BmgT120I GGNCC 2 cut(s) 224, 545
BmiI GGNNCC 2 cut(s) 6, 172
BmrI ACTGGG 1 cut(s) 176
BmsI GCATC 2 cut(s) 350, 604
BmuI ACTGGG 1 cut(s) 176
BpmI CTGGAG 2 cut(s) 105, 348
BpuEI CTTGAG 1 cut(s) 267
BsaWI WCCGGW 2 cut(s) 160, 173
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 253, 286
Bse1I ACTGG 2 cut(s) 22, 171
BseGI GGATG 1 cut(s) 538
BseLI CCNNNNNNNGG 3 cut(s) 167, 253, 286
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 2 cut(s) 22, 171
BseXI GCAGC 1 cut(s) 641
BseYI CCCAGC 1 cut(s) 404
BshFI GGCC 1 cut(s) 547
BsiSI CCGG 2 cut(s) 161, 174
BslI CCNNNNNNNGG 3 cut(s) 167, 253, 286
BsnI GGCC 1 cut(s) 547
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 4 cut(s) 4, 124, 170, 241
BspANI GGCC 1 cut(s) 547
BspCNI CTCAG 1 cut(s) 129
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 6, 172
BspPI GGATC 4 cut(s) 12, 165, 178, 236
BsrI ACTGG 2 cut(s) 22, 171
BssMI GATC 4 cut(s) 4, 124, 170, 241
Bst6I CTCTTC 4 cut(s) 257, 324, 450, 585
BstAPI GCANNNNNTGC 1 cut(s) 347
BstC8I GCNNGC 2 cut(s) 339, 378
BstDEI CTNAG 2 cut(s) 137, 483
BstF5I GGATG 1 cut(s) 538
BstKTI GATC 4 cut(s) 7, 127, 173, 244
BstMBI GATC 4 cut(s) 4, 124, 170, 241
BstMWI GCNNNNNNNGC 4 cut(s) 347, 356, 365, 437
BstV1I GCAGC 1 cut(s) 641
BstX2I RGATCY 3 cut(s) 4, 124, 170
BstYI RGATCY 3 cut(s) 4, 124, 170
BsuRI GGCC 1 cut(s) 547
BtsCI GGATG 1 cut(s) 538
BtsIMutI CAGTG 1 cut(s) 15
Cac8I GCNNGC 2 cut(s) 339, 378
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 1 cut(s) 33
Cfr13I GGNCC 2 cut(s) 224, 545
Csp6I GTAC 1 cut(s) 100
CviAII CATG 2 cut(s) 34, 58
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 137, 483
DpnI GATC 4 cut(s) 6, 126, 172, 243
DpnII GATC 4 cut(s) 4, 124, 170, 241
Eam1104I CTCTTC 4 cut(s) 257, 324, 450, 585
EarI CTCTTC 4 cut(s) 257, 324, 450, 585
Eco24I GRGCYC 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 224
EcoT38I GRGCYC 1 cut(s) 57
FaeI CATG 2 cut(s) 37, 61
FaiI YATR 3 cut(s) 35, 59, 249
FatI CATG 2 cut(s) 33, 57
Fnu4HI GCNGC 1 cut(s) 655
FokI GGATG 1 cut(s) 545
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 655
GluI GCNGC 1 cut(s) 655
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 2 cut(s) 105, 348
HaeIII GGCC 1 cut(s) 547
HapII CCGG 2 cut(s) 161, 174
Hin1II CATG 2 cut(s) 37, 61
HinfI GANTC 1 cut(s) 21
HpaII CCGG 2 cut(s) 161, 174
Hpy188I TCNGA 3 cut(s) 138, 241, 662
Hpy188III TCNNGA 4 cut(s) 34, 122, 128, 284
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 2 cut(s) 341, 350
HpyF10VI GCNNNNNNNGC 4 cut(s) 347, 356, 365, 437
HpyF3I CTNAG 2 cut(s) 137, 483
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 2 cut(s) 37, 61
Kzo9I GATC 4 cut(s) 4, 124, 170, 241
Lsp1109I GCAGC 1 cut(s) 641
LweI GCATC 2 cut(s) 350, 604
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 60
MalI GATC 4 cut(s) 6, 126, 172, 243
MboI GATC 4 cut(s) 4, 124, 170, 241
MboII GAAGA 8 cut(s) 274, 336, 338, 341, 430, 467, 505, 602
MflI RGATCY 3 cut(s) 4, 124, 170
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 4 cut(s) 67, 77, 104, 192
MlyI GAGTC 1 cut(s) 15
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 8 cut(s) 198, 203, 280, 291, 408, 451, 549, 580
MseI TTAA 2 cut(s) 108, 291
MspI CCGG 2 cut(s) 161, 174
MwoI GCNNNNNNNGC 4 cut(s) 347, 356, 365, 437
NdeII GATC 4 cut(s) 4, 124, 170, 241
NlaIII CATG 2 cut(s) 37, 61
NlaIV GGNNCC 2 cut(s) 6, 172
PagI TCATGA 1 cut(s) 33
PflMI CCANNNNNTGG 1 cut(s) 253
PkrI GCNGC 1 cut(s) 656
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PspFI CCCAGC 1 cut(s) 404
PspN4I GGNNCC 2 cut(s) 6, 172
PspPI GGNCC 2 cut(s) 224, 545
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 3 cut(s) 4, 124, 170
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SaqAI TTAA 2 cut(s) 108, 291
SatI GCNGC 1 cut(s) 655
Sau3AI GATC 4 cut(s) 4, 124, 170, 241
Sau96I GGNCC 2 cut(s) 224, 545
SchI GAGTC 1 cut(s) 15
SduI GDGCHC 1 cut(s) 57
SetI ASST 5 cut(s) 42, 117, 291, 361, 433
SfaNI GCATC 2 cut(s) 350, 604
SinI GGWCC 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
Sse9I AATT 4 cut(s) 67, 77, 104, 192
TaiI ACGT 1 cut(s) 42
TaqI TCGA 1 cut(s) 51
TasI AATT 4 cut(s) 67, 77, 104, 192
Tru1I TTAA 2 cut(s) 108, 291
Tru9I TTAA 2 cut(s) 108, 291
TscAI CASTG 1 cut(s) 22
TseI GCWGC 1 cut(s) 654
TspDTI ATGAA 4 cut(s) 22, 50, 60, 549
TspRI CASTG 1 cut(s) 22
Van91I CCANNNNNTGG 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.