FvH4_2g33990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017515
Physical Location & Seq
Reverse (-)
25344720 .. 25347490
2771 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33990.t1

Sequence Viewer

Length: 1428 bp
ATGGGGCAGATACAGTTGGTTGTGGCGTTGCTAGTCTGCCTTGGAGTACTCCTCGCTGCCTTTGTTGTTCTGATACTATGGAGGTTGAATTGTTGTTGTAAGAAACGAAAAGAAACGCCGCCGAGGAGGGTCAATATTAGGAGTGGCCAGAGCTTGGAGGCCGGAATAGCAAAGCTTCATACTAACCAGCAAGAGGGTCTGCAGCAACATCATCAGTTTGATCATGTGTCTAGTAAGAAAACTGGTAACAACTATTACATGTATGGAAAGTCGTCTGCGCTTTTTAGTTGGTCGGATCATCCTTCCTCCGTCACTGACGCCGTCGAAAACGGCTGGTCTCGGTTCGCTTTTGCGACGGCTTACACGTCATCGCCGTCGACGCGATCGAGGCGGATTTTAGGGTTGTGCGCTGTTGGTGAGCATAGAGTGAGGGAGACGGAGCCGGAGATTAGTTGGGAGGTTTGTCAAGCCTCGGCGGATTTCATGCAGAAGATCAGATTAAACTCCGGCCTGAAAAAGGTGTTGAATTCCGGGAGCACCACCGGAGCTGCTGCCTCTGTGATTAGGACTGCTCTTCCTTTGCCGGGGCCACCTTTAGGGAACAACTCATTTCCTCAAGAGGCTTATTTTGAGATCACAGTTTTGTTTTCTCTTGGAGATGAAGTAAATAGTGAGGGTGAGAAGACCAAGCTCATCAAAGACAAGTTCATTGAGATGTCTAGTTCAGAATCCTTAGTACATGTCACTAGTAGTGCGCAGAGAAATAATAAAATTGAGGAAATGAAGCTTGGTGTTATAGATGAACATGATCAGAGAAGAGAAGCAGCTGTGTTACTGTCATTGGGGCTCACTACAGGCGGCTCTCTTCCGACAAAACTTCCCGGCAGCTATCCGGGAAGTATCGGATTCGGATCCAACGGTGCTGTCCATCTTGATGGGATTAAACTTGCATTTGAATCGGATAAAGCTGCATGGGGAAGGACTGACCATGTAATCGGTTGTGGATTTGATCCAAGACAAAAGAAGGTTTACTTCACTGTGGACTCGGAGCTAGTTCATGTGGTGCAGTGCAAGTCAGAGGAGTTTGGGACTCCTCTGTACCCAACTCTTGCAGCAAACACAGACATTGAGGTGTTGGTCAATTTTGGACAAAGTGTGTTCAAATATGCACCAGCAAATGCGCACAGGACCCCCAATCCCTGCTTTGTTGGTCCTTTAGGAAATGCCAACTCTCATGCCTTTTATGAAGATAGCAAAGAGCTGTTTTCGATGGGAAGGATTGATTCTCAGTGGCTTAATCGGAGTACAACCAGAGGCAGCCAGTATCCAGTCACTGCAAACCGGACGTTGGATTTTGATGAAGAGTCCAATGATCTATTCGAGATTGTTTTGGATAGTTGTGGAAGATCCCCAAACCCCGCGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPRY

Protein Analysis

476

Amino Acids

52.1

Weight (kDa)

6.68

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06536
fragaria_vesca FvH4_2g33990
malus_domestica MD08G1095800.v1.1 MD15G1078600.v1.1
prunus_persica Prupe.1G431800_v2.0.a1
pyrus_communis pycom08g07780 pycom08g07790 pycom15g07320
rosa_chinensis RchiOBHm_Chr6g0308391
rosa_multiflora Rmu_co8453189.1_g000001 Rmu_sc0005523.1_g000018
rosa_samantha Rh6AG475900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 756, 1182
AccB7I CCANNNNNTGG 1 cut(s) 154
AccI GTMKAC 1 cut(s) 377
AccII CGCG 2 cut(s) 382, 1421
AciI CCGC 5 cut(s) 119, 391, 476, 858, 1419
AclWI GGATC 5 cut(s) 303, 906, 919, 1004, 1401
AcoI YGGCCR 1 cut(s) 145
AcsI RAATTY 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 4 cut(s) 48, 738, 1100, 1306
AfiI CCNNNNNNNGG 6 cut(s) 154, 193, 517, 584, 596, 1348
AflIII ACRYGT 3 cut(s) 258, 363, 739
AgsI TTSAA 4 cut(s) 88, 526, 956, 1162
AhlI ACTAGT 1 cut(s) 746
AjiI CACGTC 1 cut(s) 366
AjuI GAANNNNNNNTTGG 4 cut(s) 771, 803, 1361, 1393
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 2 cut(s) 342, 428
AlwI GGATC 5 cut(s) 303, 906, 919, 1004, 1401
AlwNI CAGNNNCTG 1 cut(s) 1334
AoxI GGCC 4 cut(s) 145, 159, 508, 587
ApeKI GCWGC 9 cut(s) 56, 202, 548, 551, 824, 885, 968, 1112, 1317
ApoI RAATTY 1 cut(s) 526
AspLEI GCGC 4 cut(s) 280, 410, 757, 1183
AspS9I GGNCC 3 cut(s) 587, 1188, 1211
AsuC2I CCSGG 4 cut(s) 532, 585, 882, 894
AsuHPI GGTGA 2 cut(s) 428, 689
AvaII GGWCC 2 cut(s) 1188, 1211
BalI TGGCCA 1 cut(s) 147
BamHI GGATCC 1 cut(s) 911
BanII GRGCYC 1 cut(s) 849
BbsI GAAGAC 1 cut(s) 689
Bbv12I GWGCWC 1 cut(s) 539
BbvI GCAGC 9 cut(s) 43, 214, 535, 538, 836, 897, 955, 1124, 1329
BccI CCATC 3 cut(s) 929, 936, 1264
BceAI ACGGC 4 cut(s) 305, 346, 358, 372
BcgI CGANNNNNNTGC 2 cut(s) 939, 973
BciVI GTATCC 1 cut(s) 1335
BclI TGATCA 2 cut(s) 220, 808
BcnI CCSGG 4 cut(s) 532, 585, 882, 894
BcoDI GTCTC 2 cut(s) 342, 428
BcuI ACTAGT 1 cut(s) 746
BfaI CTAG 5 cut(s) 32, 231, 720, 747, 1052
BfmI CTRYAG 2 cut(s) 200, 852
BfuI GTATCC 1 cut(s) 1335
BmcAI AGTACT 1 cut(s) 48
Bme1390I CCNGG 4 cut(s) 532, 585, 882, 894
Bme18I GGWCC 2 cut(s) 1188, 1211
BmgBI CACGTC 1 cut(s) 366
BmgT120I GGNCC 3 cut(s) 587, 1188, 1211
BmiI GGNNCC 4 cut(s) 441, 588, 913, 1190
BmrFI CCNGG 4 cut(s) 532, 585, 882, 894
BpiI GAAGAC 1 cut(s) 689
BplI GAGNNNNNCTC 2 cut(s) 36, 68
BpuEI CTTGAG 1 cut(s) 600
BpuMI CCSGG 4 cut(s) 532, 585, 882, 894
BsaBI GATNNNNATC 1 cut(s) 910
BsaHI GRCGYC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 342
BsaJI CCNNGG 4 cut(s) 40, 122, 471, 584
BsaWI WCCGGW 2 cut(s) 542, 1341
BsaXI ACNNNNNCTCC 2 cut(s) 648, 678
Bsc4I CCNNNNNNNGG 6 cut(s) 154, 193, 517, 584, 596, 1348
Bse1I ACTGG 3 cut(s) 247, 1321, 1328
Bse8I GATNNNNATC 1 cut(s) 910
BseDI CCNNGG 4 cut(s) 40, 122, 471, 584
BseGI GGATG 1 cut(s) 298
BseJI GATNNNNATC 1 cut(s) 910
BseLI CCNNNNNNNGG 6 cut(s) 154, 193, 517, 584, 596, 1348
BseMII CTCAG 1 cut(s) 1301
BseNI ACTGG 3 cut(s) 247, 1321, 1328
BseRI GAGGAG 4 cut(s) 41, 139, 1083, 1094
BseXI GCAGC 9 cut(s) 43, 214, 535, 538, 836, 897, 955, 1124, 1329
BsgI GTGCAG 1 cut(s) 1085
Bsh1236I CGCG 2 cut(s) 382, 1421
Bsh1285I CGRYCG 1 cut(s) 386
BshFI GGCC 4 cut(s) 147, 161, 510, 589
BsiEI CGRYCG 1 cut(s) 386
BsiHKAI GWGCWC 1 cut(s) 539
BsiSI CCGG 9 cut(s) 162, 443, 507, 531, 543, 584, 882, 893, 1342
BslFI GGGAC 1 cut(s) 1102
BslI CCNNNNNNNGG 6 cut(s) 154, 193, 517, 584, 596, 1348
BsmAI GTCTC 2 cut(s) 342, 428
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 1102
BsnI GGCC 4 cut(s) 147, 161, 510, 589
Bso31I GGTCTC 1 cut(s) 342
Bsp1286I GDGCHC 2 cut(s) 539, 849
BspACI CCGC 5 cut(s) 119, 391, 476, 858, 1419
BspANI GGCC 4 cut(s) 147, 161, 510, 589
BspCNI CTCAG 1 cut(s) 1300
BspFNI CGCG 2 cut(s) 382, 1421
BspLI GGNNCC 4 cut(s) 441, 588, 913, 1190
BspMAI CTGCAG 1 cut(s) 204
BspPI GGATC 5 cut(s) 303, 906, 919, 1004, 1401
BspQI GCTCTTC 1 cut(s) 579
BspTNI GGTCTC 1 cut(s) 342
BsrI ACTGG 3 cut(s) 247, 1321, 1328
BssECI CCNNGG 4 cut(s) 40, 122, 471, 584
BssNI GRCGYC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 40
Bst4CI ACNGT 5 cut(s) 15, 640, 837, 920, 1039
Bst6I CTCTTC 4 cut(s) 579, 811, 870, 1356
BstACI GRCGYC 1 cut(s) 318
BstDEI CTNAG 2 cut(s) 733, 1287
BstENI CCTNNNNNAGG 1 cut(s) 515
BstF5I GGATG 1 cut(s) 298
BstFNI CGCG 2 cut(s) 382, 1421
BstHHI GCGC 4 cut(s) 280, 410, 757, 1183
BstMAI GTCTC 2 cut(s) 342, 428
BstMCI CGRYCG 1 cut(s) 386
BstMWI GCNNNNNNNGC 3 cut(s) 167, 379, 388
BstNSI RCATGY 2 cut(s) 262, 743
BstSCI CCNGG 4 cut(s) 530, 583, 880, 892
BstSFI CTRYAG 2 cut(s) 200, 852
BstUI CGCG 2 cut(s) 382, 1421
BstV1I GCAGC 9 cut(s) 43, 214, 535, 538, 836, 897, 955, 1124, 1329
BstV2I GAAGAC 1 cut(s) 689
BstX2I RGATCY 2 cut(s) 911, 1406
BstXI CCANNNNNNTGG 1 cut(s) 935
BstYI RGATCY 2 cut(s) 911, 1406
BsuI GTATCC 1 cut(s) 1335
BsuRI GGCC 4 cut(s) 147, 161, 510, 589
BtgZI GCGATG 1 cut(s) 354
BtrI CACGTC 1 cut(s) 366
BtsCI GGATG 1 cut(s) 298
BtsI GCAGTG 2 cut(s) 1073, 1332
BtsIMutI CAGTG 5 cut(s) 312, 1035, 1073, 1295, 1332
CaiI CAGNNNCTG 1 cut(s) 1334
CfoI GCGC 4 cut(s) 280, 410, 757, 1183
Cfr13I GGNCC 3 cut(s) 587, 1188, 1211
CseI GACGC 2 cut(s) 326, 388
Csp6I GTAC 4 cut(s) 47, 737, 1099, 1305
CviAII CATG 9 cut(s) 224, 259, 484, 740, 806, 972, 989, 1058, 1235
CviQI GTAC 4 cut(s) 47, 737, 1099, 1305
DdeI CTNAG 2 cut(s) 733, 1287
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 4 cut(s) 579, 811, 870, 1356
EarI CTCTTC 4 cut(s) 579, 811, 870, 1356
EciI GGCGGA 2 cut(s) 406, 491
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 849
Eco31I GGTCTC 1 cut(s) 342
Eco47I GGWCC 2 cut(s) 1188, 1211
EcoNI CCTNNNNNAGG 1 cut(s) 515
EcoO109I RGGNCCY 1 cut(s) 1188
EcoRI GAATTC 1 cut(s) 526
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 849
ErhI CCWWGG 1 cut(s) 40
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 9 cut(s) 227, 262, 487, 743, 809, 975, 992, 1061, 1238
FalI AAGNNNNNCTT 2 cut(s) 1016, 1048
FaqI GGGAC 1 cut(s) 1102
FatI CATG 9 cut(s) 223, 258, 483, 739, 805, 971, 988, 1057, 1234
FauI CCCGC 1 cut(s) 1426
FbaI TGATCA 2 cut(s) 220, 808
FblI GTMKAC 1 cut(s) 377
FokI GGATG 1 cut(s) 285
FriOI GRGCYC 1 cut(s) 849
FspAI RTGCGCAY 1 cut(s) 1182
FspBI CTAG 5 cut(s) 32, 231, 720, 747, 1052
FspI TGCGCA 2 cut(s) 756, 1182
GlaI GCGC 4 cut(s) 279, 409, 756, 1182
HaeIII GGCC 4 cut(s) 147, 161, 510, 589
HapII CCGG 9 cut(s) 162, 443, 507, 531, 543, 584, 882, 893, 1342
HgaI GACGC 2 cut(s) 326, 388
HhaI GCGC 4 cut(s) 280, 410, 757, 1183
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 9 cut(s) 227, 262, 487, 743, 809, 975, 992, 1061, 1238
Hin6I GCGC 4 cut(s) 278, 408, 755, 1181
HinP1I GCGC 4 cut(s) 278, 408, 755, 1181
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HindIII AAGCTT 2 cut(s) 173, 785
HinfI GANTC 7 cut(s) 728, 906, 956, 1043, 1090, 1283, 1364
HpaII CCGG 9 cut(s) 162, 443, 507, 531, 543, 584, 882, 893, 1342
HphI GGTGA 2 cut(s) 428, 689
Hpy166II GTNNAC 3 cut(s) 378, 1030, 1042
Hpy188III TCNNGA 3 cut(s) 617, 932, 1381
Hpy8I GTNNAC 3 cut(s) 378, 1030, 1042
Hpy99I CGWCG 4 cut(s) 326, 358, 379, 382
HpyAV CCTTC 4 cut(s) 312, 972, 1018, 1269
HpyCH4III ACNGT 5 cut(s) 15, 640, 837, 920, 1039
HpyCH4IV ACGT 2 cut(s) 365, 1346
HpyCH4V TGCA 9 cut(s) 202, 487, 950, 971, 1066, 1071, 1112, 1169, 1337
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 379, 388
HpyF3I CTNAG 2 cut(s) 733, 1287
HpySE526I ACGT 2 cut(s) 365, 1346
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 9 cut(s) 227, 262, 487, 743, 809, 975, 992, 1061, 1238
HspAI GCGC 4 cut(s) 278, 408, 755, 1181
Ksp22I TGATCA 2 cut(s) 220, 808
LguI GCTCTTC 1 cut(s) 579
LmnI GCTCC 4 cut(s) 439, 534, 545, 1048
Lsp1109I GCAGC 9 cut(s) 43, 214, 535, 538, 836, 897, 955, 1124, 1329
MaeI CTAG 5 cut(s) 32, 231, 720, 747, 1052
MaeII ACGT 2 cut(s) 365, 1346
MaeIII GTNAC 5 cut(s) 245, 310, 742, 831, 1330
MboII GAAGA 8 cut(s) 502, 566, 694, 828, 857, 1259, 1373, 1416
MflI RGATCY 2 cut(s) 911, 1406
MhlI GDGCHC 2 cut(s) 539, 849
MlsI TGGCCA 1 cut(s) 147
MluCI AATT 4 cut(s) 88, 526, 771, 1141
MluNI TGGCCA 1 cut(s) 147
MlyI GAGTC 3 cut(s) 1037, 1084, 1373
MmeI TCCRAC 4 cut(s) 273, 893, 939, 1329
Mox20I TGGCCA 1 cut(s) 147
MscI TGGCCA 1 cut(s) 147
MseI TTAA 3 cut(s) 500, 942, 1296
MslI CAYNNNNRTG 3 cut(s) 713, 933, 1130
Msp20I TGGCCA 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 827
MspI CCGG 9 cut(s) 162, 443, 507, 531, 543, 584, 882, 893, 1342
MspR9I CCNGG 4 cut(s) 532, 585, 882, 894
MvnI CGCG 2 cut(s) 382, 1421
MwoI GCNNNNNNNGC 3 cut(s) 167, 379, 388
NciI CCSGG 4 cut(s) 532, 585, 882, 894
NlaIII CATG 9 cut(s) 227, 262, 487, 743, 809, 975, 992, 1061, 1238
NlaIV GGNNCC 4 cut(s) 441, 588, 913, 1190
NmeAIII GCCGAG 2 cut(s) 147, 452
NmuCI GTSAC 3 cut(s) 310, 742, 1330
NsbI TGCGCA 2 cut(s) 756, 1182
NspI RCATGY 2 cut(s) 262, 743
PciI ACATGT 2 cut(s) 258, 739
PciSI GCTCTTC 1 cut(s) 579
PcsI WCGNNNNNNNCGW 3 cut(s) 362, 371, 383
PfeI GAWTC 4 cut(s) 728, 906, 956, 1283
PflFI GACNNNGTC 1 cut(s) 320
PflMI CCANNNNNTGG 1 cut(s) 154
PfoI TCCNGGA 2 cut(s) 530, 892
Ple19I CGATCG 1 cut(s) 386
PleI GAGTC 3 cut(s) 1037, 1084, 1372
PpsI GAGTC 3 cut(s) 1037, 1084, 1372
PpuMI RGGWCCY 1 cut(s) 1188
PscI ACATGT 2 cut(s) 258, 739
Psp5II RGGWCCY 1 cut(s) 1188
PspN4I GGNNCC 4 cut(s) 441, 588, 913, 1190
PspPI GGNCC 3 cut(s) 587, 1188, 1211
PspPPI RGGWCCY 1 cut(s) 1188
PstI CTGCAG 1 cut(s) 204
PstNI CAGNNNCTG 1 cut(s) 1334
PsuI RGATCY 2 cut(s) 911, 1406
PsyI GACNNNGTC 1 cut(s) 320
PvuI CGATCG 1 cut(s) 386
PvuII CAGCTG 1 cut(s) 827
RsaI GTAC 4 cut(s) 48, 738, 1100, 1306
RsaNI GTAC 4 cut(s) 47, 737, 1099, 1305
RseI CAYNNNNRTG 3 cut(s) 713, 933, 1130
SalI GTCGAC 1 cut(s) 376
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 3 cut(s) 500, 942, 1296
Sau96I GGNCC 3 cut(s) 587, 1188, 1211
ScaI AGTACT 1 cut(s) 48
SchI GAGTC 3 cut(s) 1037, 1084, 1373
ScrFI CCNGG 4 cut(s) 532, 585, 882, 894
SduI GDGCHC 2 cut(s) 539, 849
SfcI CTRYAG 2 cut(s) 200, 852
SgrDI CGTCGACG 1 cut(s) 376
SinI GGWCC 2 cut(s) 1188, 1211
SmiMI CAYNNNNRTG 3 cut(s) 713, 933, 1130
SmlI CTYRAG 1 cut(s) 615
SmoI CTYRAG 1 cut(s) 615
SpeI ACTAGT 1 cut(s) 746
Sse9I AATT 4 cut(s) 88, 526, 771, 1141
SsiI CCGC 5 cut(s) 119, 391, 476, 858, 1419
SspI AATATT 1 cut(s) 136
SspMI CTAG 5 cut(s) 32, 231, 720, 747, 1052
StyD4I CCNGG 4 cut(s) 530, 583, 880, 892
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 5 cut(s) 15, 640, 837, 920, 1039
TaiI ACGT 2 cut(s) 368, 1349
TaqI TCGA 5 cut(s) 324, 377, 386, 1268, 1380
TasI AATT 4 cut(s) 88, 526, 771, 1141
TatI WGTACW 3 cut(s) 46, 736, 1304
TauI GCSGC 2 cut(s) 121, 861
TfiI GAWTC 4 cut(s) 728, 906, 956, 1283
Tru1I TTAA 3 cut(s) 500, 942, 1296
Tru9I TTAA 3 cut(s) 500, 942, 1296
TscAI CASTG 5 cut(s) 319, 1042, 1073, 1295, 1339
TseFI GTSAC 3 cut(s) 310, 742, 1330
TseI GCWGC 9 cut(s) 56, 202, 548, 551, 824, 885, 968, 1112, 1317
Tsp45I GTSAC 3 cut(s) 310, 742, 1330
TspDTI ATGAA 9 cut(s) 167, 472, 675, 697, 797, 816, 1046, 1260, 1374
TspGWI ACGGA 2 cut(s) 298, 452
TspRI CASTG 5 cut(s) 319, 1042, 1073, 1295, 1339
Tth111I GACNNNGTC 1 cut(s) 320
Van91I CCANNNNNTGG 1 cut(s) 154
VpaK11BI GGWCC 2 cut(s) 1188, 1211
XagI CCTNNNNNAGG 1 cut(s) 515
XapI RAATTY 1 cut(s) 526
XceI RCATGY 2 cut(s) 262, 743
XmiI GTMKAC 1 cut(s) 377
XspI CTAG 5 cut(s) 32, 231, 720, 747, 1052
ZrmI AGTACT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.