FvH4_2g36420

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015453
Physical Location & Seq
Reverse (-)
26673046 .. 26675570
2525 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36420.t1

Sequence Viewer

Length: 711 bp
ATGGAATGGAGAAAATGCTATTTGGATGTGATTCTGGTTCCTCTAGGGTTTCTGATAATGGCTGGTTATCATGGATGGTTGTGGCACAATGTTCGTACTAAACCCGAGACTACTATCATCGGCATAAACACCAGAGGAAGACGATTATGGGTTTCTGCAATGATCAAGGACAATGAAAAGAAGAACATCCTGGCAGTCCAAACCCTTCGAAACACGATCATGGGATCAACCCTAATGGCCACCACGTCGATCCTTCTCTGCTCCGGCCTGGCGGCCGTGATCAGCAGCACGTACAGTGTAAAGAAGCCGCTGAACGATGCCGTCTACGGCGCTCACGGGGAGTTCATGGTGGCCCTCAAATACGTGACCCTACTCACCATTTTCCTCTTCTCCTTCATGTGTCACTCGCTTTCCATCAGGTTCATAAATCAAGTGAACATCCTCATCAACAGTCCTCAAGACCCTACTTCTCCGGTGACCCCCGAGTACGTGTCGGAGCTTCTAGAGAAAGGTTACATTCTGAACACGGTGGGGAACAGGCTGTTCTACGCAGCACTACCTCTTCTGCTTTGGATTTTTGGTCCGGTGCTGGTGTTCTTGTGCTCCATTACCTTGGTGCCGATGCTTTACAATTTGGATTTTGTGCTTAGAAGCCCTGATCAGAAAGGAAAGAACATGGATGCTTCTGGAAATGATTCGGATTTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

237

Amino Acids

26.52

Weight (kDa)

8.86

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 215 1.3e-77 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015453)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31330
fragaria_vesca FvH4_2g36420
malus_domestica MD08G1123900.v1.1 MD15G1102500.v1.1
prunus_persica Prupe.1G455600_v2.0.a1
pyrus_communis pycom08g10270
rosa_chinensis RchiOBHm_Chr6g0305481
rosa_laevigata RLG00000010888
rosa_multiflora Rmu_sc0007817.1_g000001
rosa_roxburghii Rroxscaffold_7G00162770
rosa_rugosa Rorug06G0340400
rosa_samantha Rh6AG452600 Rh6BG449200 Rh6CG466300 Rh6DG453000
rosa_wichuraiana Rw6G039440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 616
AccI GTMKAC 1 cut(s) 324
AciI CCGC 2 cut(s) 272, 308
AclWI GGATC 2 cut(s) 232, 244
AcoI YGGCCR 2 cut(s) 237, 273
AfaI GTAC 3 cut(s) 97, 293, 488
AflIII ACRYGT 1 cut(s) 489
AjiI CACGTC 1 cut(s) 246
AjnI CCWGG 2 cut(s) 189, 267
AluBI AGCT 1 cut(s) 499
AluI AGCT 1 cut(s) 499
Alw21I GWGCWC 1 cut(s) 605
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 2 cut(s) 232, 244
Ama87I CYCGRG 2 cut(s) 104, 482
AoxI GGCC 4 cut(s) 237, 265, 273, 351
ApeKI GCWGC 2 cut(s) 285, 551
Asp700I GAANNNNTTC 1 cut(s) 694
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 352, 581
AsuHPI GGTGA 2 cut(s) 367, 487
AsuII TTCGAA 1 cut(s) 208
AvaI CYCGRG 2 cut(s) 104, 482
AvaII GGWCC 1 cut(s) 581
BalI TGGCCA 1 cut(s) 239
BanI GGYRCC 1 cut(s) 616
BbsI GAAGAC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 605
BbvI GCAGC 2 cut(s) 297, 563
BccI CCATC 2 cut(s) 69, 422
BceAI ACGGC 3 cut(s) 260, 305, 343
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 2 cut(s) 191, 269
BclI TGATCA 3 cut(s) 162, 279, 658
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 2 cut(s) 44, 503
BfoI RGCGCY 1 cut(s) 333
BisI GCNGC 4 cut(s) 273, 286, 308, 552
BlsI GCNGC 4 cut(s) 274, 287, 309, 553
Bme1390I CCNGG 2 cut(s) 191, 269
Bme18I GGWCC 1 cut(s) 581
BmeT110I CYCGRG 2 cut(s) 104, 482
BmgBI CACGTC 1 cut(s) 246
BmgT120I GGNCC 2 cut(s) 352, 581
BmiI GGNNCC 2 cut(s) 39, 618
BmrFI CCNGG 2 cut(s) 191, 269
BmsI GCATC 3 cut(s) 307, 612, 670
BpiI GAAGAC 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 441
BsaAI YACGTR 3 cut(s) 291, 364, 490
BsaJI CCNNGG 1 cut(s) 612
BsaWI WCCGGW 2 cut(s) 472, 583
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 2 cut(s) 191, 269
BseDI CCNNGG 1 cut(s) 612
BseGI GGATG 5 cut(s) 31, 80, 186, 438, 685
BseMI GCAATG 1 cut(s) 165
BseX3I CGGCCG 1 cut(s) 273
BseXI GCAGC 2 cut(s) 297, 563
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 4 cut(s) 239, 267, 275, 353
BshNI GGYRCC 1 cut(s) 616
BsiEI CGRYCG 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 605
BsiHKCI CYCGRG 2 cut(s) 104, 482
BsiSI CCGG 3 cut(s) 264, 473, 584
BsmAI GTCTC 1 cut(s) 101
BsnI GGCC 4 cut(s) 239, 267, 275, 353
BsoBI CYCGRG 2 cut(s) 104, 482
Bsp119I TTCGAA 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 605
Bsp143I GATC 6 cut(s) 162, 216, 224, 249, 279, 658
BspACI CCGC 2 cut(s) 272, 308
BspANI GGCC 4 cut(s) 239, 267, 275, 353
BspLI GGNNCC 2 cut(s) 39, 618
BspPI GGATC 2 cut(s) 232, 244
BspT104I TTCGAA 1 cut(s) 208
BspT107I GGYRCC 1 cut(s) 616
BsrDI GCAATG 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 612
BssMI GATC 6 cut(s) 162, 216, 224, 249, 279, 658
BssT1I CCWWGG 1 cut(s) 612
Bst2UI CCWGG 2 cut(s) 191, 269
Bst4CI ACNGT 3 cut(s) 296, 452, 529
Bst6I CTCTTC 2 cut(s) 392, 567
BstBAI YACGTR 3 cut(s) 291, 364, 490
BstBI TTCGAA 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 647
BstEII GGTNACC 1 cut(s) 475
BstF5I GGATG 5 cut(s) 31, 80, 186, 438, 685
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 6 cut(s) 165, 219, 227, 252, 282, 661
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 6 cut(s) 162, 216, 224, 249, 279, 658
BstMCI CGRYCG 1 cut(s) 276
BstNI CCWGG 2 cut(s) 191, 269
BstPI GGTNACC 1 cut(s) 475
BstSCI CCNGG 2 cut(s) 189, 267
BstV1I GCAGC 2 cut(s) 297, 563
BstV2I GAAGAC 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 613
BstZI CGGCCG 1 cut(s) 273
BsuRI GGCC 4 cut(s) 239, 267, 275, 353
BtrI CACGTC 1 cut(s) 246
BtsCI GGATG 5 cut(s) 31, 80, 186, 438, 685
BtsIMutI CAGTG 1 cut(s) 301
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 2 cut(s) 352, 581
Csp6I GTAC 3 cut(s) 96, 292, 487
CviAII CATG 5 cut(s) 71, 220, 346, 397, 676
CviJI RGCY 9 cut(s) 62, 239, 267, 275, 307, 353, 499, 541, 654
CviKI_1 RGCY 9 cut(s) 62, 239, 267, 275, 307, 353, 499, 541, 654
CviQI GTAC 3 cut(s) 96, 292, 487
DdeI CTNAG 1 cut(s) 647
DpnI GATC 6 cut(s) 164, 218, 226, 251, 281, 660
DpnII GATC 6 cut(s) 162, 216, 224, 249, 279, 658
EaeI YGGCCR 2 cut(s) 237, 273
EagI CGGCCG 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 392, 567
EarI CTCTTC 2 cut(s) 392, 567
EclXI CGGCCG 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 612
Eco47I GGWCC 1 cut(s) 581
Eco52I CGGCCG 1 cut(s) 273
Eco88I CYCGRG 2 cut(s) 104, 482
Eco91I GGTNACC 1 cut(s) 475
EcoO65I GGTNACC 1 cut(s) 475
EcoRII CCWGG 2 cut(s) 189, 267
EcoT14I CCWWGG 1 cut(s) 612
ErhI CCWWGG 1 cut(s) 612
FaeI CATG 5 cut(s) 74, 223, 349, 400, 679
FaiI YATR 8 cut(s) 72, 125, 148, 221, 347, 398, 425, 677
FatI CATG 5 cut(s) 70, 219, 345, 396, 675
FbaI TGATCA 3 cut(s) 162, 279, 658
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 4 cut(s) 273, 286, 308, 552
FokI GGATG 5 cut(s) 38, 87, 173, 425, 692
Fsp4HI GCNGC 4 cut(s) 273, 286, 308, 552
FspBI CTAG 2 cut(s) 44, 503
GlaI GCGC 1 cut(s) 331
GluI GCNGC 4 cut(s) 273, 286, 308, 552
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 4 cut(s) 239, 267, 275, 353
HapII CCGG 3 cut(s) 264, 473, 584
HhaI GCGC 1 cut(s) 332
Hin1II CATG 5 cut(s) 74, 223, 349, 400, 679
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 2 cut(s) 31, 695
HpaII CCGG 3 cut(s) 264, 473, 584
HphI GGTGA 2 cut(s) 367, 487
Hpy166II GTNNAC 2 cut(s) 325, 436
Hpy188I TCNGA 5 cut(s) 54, 496, 522, 663, 700
Hpy188III TCNNGA 3 cut(s) 458, 503, 687
Hpy8I GTNNAC 2 cut(s) 325, 436
Hpy99I CGWCG 1 cut(s) 250
HpyAV CCTTC 3 cut(s) 215, 263, 403
HpyCH4III ACNGT 3 cut(s) 296, 452, 529
HpyCH4IV ACGT 4 cut(s) 245, 290, 363, 489
HpyCH4V TGCA 1 cut(s) 158
HpyF3I CTNAG 1 cut(s) 647
HpySE526I ACGT 4 cut(s) 245, 290, 363, 489
Hsp92II CATG 5 cut(s) 74, 223, 349, 400, 679
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 3 cut(s) 162, 279, 658
Kzo9I GATC 6 cut(s) 162, 216, 224, 249, 279, 658
LmnI GCTCC 3 cut(s) 266, 496, 608
Lsp1109I GCAGC 2 cut(s) 297, 563
LweI GCATC 3 cut(s) 307, 612, 670
MaeI CTAG 2 cut(s) 44, 503
MaeII ACGT 4 cut(s) 245, 290, 363, 489
MaeIII GTNAC 4 cut(s) 364, 401, 475, 512
MalI GATC 6 cut(s) 164, 218, 226, 251, 281, 660
MboI GATC 6 cut(s) 162, 216, 224, 249, 279, 658
MboII GAAGA 4 cut(s) 150, 193, 379, 554
MhlI GDGCHC 1 cut(s) 605
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 1 cut(s) 631
MluNI TGGCCA 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 7 cut(s) 51, 128, 365, 395, 452, 465, 570
Mox20I TGGCCA 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 694
MscI TGGCCA 1 cut(s) 239
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 3 cut(s) 264, 473, 584
MspR9I CCNGG 2 cut(s) 191, 269
MvaI CCWGG 2 cut(s) 191, 269
NdeII GATC 6 cut(s) 162, 216, 224, 249, 279, 658
NlaIII CATG 5 cut(s) 74, 223, 349, 400, 679
NlaIV GGNNCC 2 cut(s) 39, 618
NmuCI GTSAC 3 cut(s) 364, 401, 475
NspV TTCGAA 1 cut(s) 208
PdmI GAANNNNTTC 1 cut(s) 694
PfeI GAWTC 2 cut(s) 31, 695
PkrI GCNGC 4 cut(s) 274, 287, 309, 553
Ppu21I YACGTR 3 cut(s) 291, 364, 490
Psp6I CCWGG 2 cut(s) 189, 267
PspEI GGTNACC 1 cut(s) 475
PspGI CCWGG 2 cut(s) 189, 267
PspN4I GGNNCC 2 cut(s) 39, 618
PspPI GGNCC 2 cut(s) 352, 581
RsaI GTAC 3 cut(s) 97, 293, 488
RsaNI GTAC 3 cut(s) 96, 292, 487
RseI CAYNNNNRTG 1 cut(s) 218
SatI GCNGC 4 cut(s) 273, 286, 308, 552
Sau3AI GATC 6 cut(s) 162, 216, 224, 249, 279, 658
Sau96I GGNCC 2 cut(s) 352, 581
ScrFI CCNGG 2 cut(s) 191, 269
SduI GDGCHC 1 cut(s) 605
SetI ASST 9 cut(s) 248, 293, 366, 422, 492, 501, 514, 562, 614
SfaNI GCATC 3 cut(s) 307, 612, 670
SfuI TTCGAA 1 cut(s) 208
SinI GGWCC 1 cut(s) 581
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 456
SmoI CTYRAG 1 cut(s) 456
Sse9I AATT 1 cut(s) 631
SsiI CCGC 2 cut(s) 272, 308
SspMI CTAG 2 cut(s) 44, 503
StyD4I CCNGG 2 cut(s) 189, 267
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 3 cut(s) 296, 452, 529
TaiI ACGT 4 cut(s) 248, 293, 366, 492
TaqI TCGA 2 cut(s) 208, 248
TasI AATT 1 cut(s) 631
TauI GCSGC 2 cut(s) 275, 310
TfiI GAWTC 2 cut(s) 31, 695
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 3 cut(s) 364, 401, 475
TseI GCWGC 2 cut(s) 285, 551
Tsp45I GTSAC 3 cut(s) 364, 401, 475
TspDTI ATGAA 4 cut(s) 189, 334, 385, 412
TspRI CASTG 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 581
XbaI TCTAGA 1 cut(s) 502
XmiI GTMKAC 1 cut(s) 324
XmnI GAANNNNTTC 1 cut(s) 694
XspI CTAG 2 cut(s) 44, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.