MD08G1123900.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015453
Physical Location & Seq
Reverse (-)
11608328 .. 11610475
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1123900.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGAATGGAGAAAATGCTACTTGGATGTCATTCTTGTACCACTAGGGTTTCTCATAACTGTTTCTTATCATGCTTGGTTATGGCATAAAGTTCGGACTGACCCACGATCTACCATCATCGGCATAAACACCAGCGGACGACGATTCTGGGTCTCGGCAATGATGAAGGACAATGAAAAGAAGAATATCCTGGCCGTCCAAACCCTTCGTAACACGATCATGGGATCAACCCTAATGGCCACTACATCAATCCTCTTATGCTCCGGCCTAGCAGCCGTGATCAGCAGCACATACAGTGTGAAGAAACCGCTCAATGACACTGTTTACGGAGCTCACGGGGAATTCATGGTTGCTTTAAAGTACGTTTCCCTACTCACAATTTTCCTCTTCTCATTCATGTGTCACTCGCTTTCAATCAGGTTCATAAACCAAGTGAACATCCTAATTAACAGTCCACAAGACCCCATGTCCTTGGTGACTCCAGAGTATGTGTCTGAGCTTTTAGAAAAGGGTTACTTGTTAAACACTGCGGGGAACAGGTTGTTTTATGCAGCACTGCCTCTTGTGCTTTGGATATTTGGACCGGTTTTGGTGTTTTTGTGTTCTGTCACAATGGTTCCCGTGCTTTACAATTTGGACTTTGTGTTTAGTAGTAGCACTGAGAAGAGAAAAATGGATATTGGTAATGAAAATAGGGATGAATTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

237

Amino Acids

26.76

Weight (kDa)

8.86

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 216 2.8e-77 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015453)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31330
fragaria_vesca FvH4_2g36420
malus_domestica MD08G1123900.v1.1 MD15G1102500.v1.1
prunus_persica Prupe.1G455600_v2.0.a1
pyrus_communis pycom08g10270
rosa_chinensis RchiOBHm_Chr6g0305481
rosa_laevigata RLG00000010888
rosa_multiflora Rmu_sc0007817.1_g000001
rosa_roxburghii Rroxscaffold_7G00162770
rosa_rugosa Rorug06G0340400
rosa_samantha Rh6AG452600 Rh6BG449200 Rh6CG466300 Rh6DG453000
rosa_wichuraiana Rw6G039440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 310
AciI CCGC 3 cut(s) 135, 308, 530
AclWI GGATC 1 cut(s) 232
AcoI YGGCCR 2 cut(s) 192, 237
AcsI RAATTY 2 cut(s) 341, 701
AfaI GTAC 2 cut(s) 39, 362
AgeI ACCGGT 1 cut(s) 583
AgsI TTSAA 1 cut(s) 414
AhdI GACNNNNNGTC 1 cut(s) 466
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 2 cut(s) 332, 499
AluI AGCT 2 cut(s) 332, 499
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 1 cut(s) 157
AlwI GGATC 1 cut(s) 232
AoxI GGCC 3 cut(s) 192, 237, 265
ApeKI GCWGC 3 cut(s) 272, 285, 551
ApoI RAATTY 2 cut(s) 341, 701
AsiGI ACCGGT 1 cut(s) 583
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 1 cut(s) 487
AvaII GGWCC 1 cut(s) 581
BalI TGGCCA 1 cut(s) 239
BanII GRGCYC 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 3 cut(s) 284, 297, 563
BccI CCATC 1 cut(s) 122
BceAI ACGGC 2 cut(s) 179, 260
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 1 cut(s) 191
BclI TGATCA 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 157
BfaI CTAG 2 cut(s) 44, 269
BisI GCNGC 3 cut(s) 273, 286, 552
BlsI GCNGC 3 cut(s) 274, 287, 553
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 581
BmeRI GACNNNNNGTC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 1 cut(s) 618
BmrFI CCNGG 1 cut(s) 191
BpmI CTGGAG 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 471
BsaWI WCCGGW 1 cut(s) 583
Bse118I RCCGGY 1 cut(s) 583
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 3 cut(s) 31, 438, 703
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 2 cut(s) 486, 651
BseXI GCAGC 3 cut(s) 284, 297, 563
BshFI GGCC 3 cut(s) 194, 239, 267
BshTI ACCGGT 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 2 cut(s) 264, 584
BsmAI GTCTC 1 cut(s) 157
BsnI GGCC 3 cut(s) 194, 239, 267
Bso31I GGTCTC 1 cut(s) 157
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 4 cut(s) 107, 216, 224, 279
BspACI CCGC 3 cut(s) 135, 308, 530
BspANI GGCC 3 cut(s) 194, 239, 267
BspCNI CTCAG 2 cut(s) 487, 652
BspLI GGNNCC 1 cut(s) 618
BspPI GGATC 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 157
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 583
BssAI RCCGGY 1 cut(s) 583
BssECI CCNNGG 1 cut(s) 471
BssMI GATC 4 cut(s) 107, 216, 224, 279
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 4 cut(s) 61, 296, 322, 452
Bst6I CTCTTC 2 cut(s) 392, 659
BstDEI CTNAG 2 cut(s) 495, 660
BstF5I GGATG 3 cut(s) 31, 438, 703
BstKTI GATC 4 cut(s) 110, 219, 227, 282
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 4 cut(s) 107, 216, 224, 279
BstMWI GCNNNNNNNGC 1 cut(s) 565
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 189
BstV1I GCAGC 3 cut(s) 284, 297, 563
BstXI CCANNNNNNTGG 1 cut(s) 472
BsuRI GGCC 3 cut(s) 194, 239, 267
BtsCI GGATG 3 cut(s) 31, 438, 703
BtsI GCAGTG 2 cut(s) 525, 554
BtsIMutI CAGTG 5 cut(s) 301, 318, 525, 554, 657
Cfr10I RCCGGY 1 cut(s) 583
Cfr13I GGNCC 1 cut(s) 581
Csp6I GTAC 2 cut(s) 38, 361
CspAI ACCGGT 1 cut(s) 583
CviAII CATG 5 cut(s) 71, 220, 346, 397, 466
CviJI RGCY 6 cut(s) 194, 239, 267, 275, 332, 499
CviKI_1 RGCY 6 cut(s) 194, 239, 267, 275, 332, 499
CviQI GTAC 2 cut(s) 38, 361
DdeI CTNAG 2 cut(s) 495, 660
DpnI GATC 4 cut(s) 109, 218, 226, 281
DpnII GATC 4 cut(s) 107, 216, 224, 279
DraI TTTAAA 1 cut(s) 357
DriI GACNNNNNGTC 1 cut(s) 466
EaeI YGGCCR 2 cut(s) 192, 237
Eam1104I CTCTTC 2 cut(s) 392, 659
Eam1105I GACNNNNNGTC 1 cut(s) 466
EarI CTCTTC 2 cut(s) 392, 659
Ecl136II GAGCTC 1 cut(s) 332
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 334
Eco31I GGTCTC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 581
Eco53kI GAGCTC 1 cut(s) 332
EcoICRI GAGCTC 1 cut(s) 332
EcoRI GAATTC 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 5 cut(s) 74, 223, 349, 400, 469
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FatI CATG 5 cut(s) 70, 219, 345, 396, 465
FauI CCCGC 1 cut(s) 523
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 3 cut(s) 273, 286, 552
FokI GGATG 2 cut(s) 38, 425
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 3 cut(s) 273, 286, 552
FspBI CTAG 2 cut(s) 44, 269
GluI GCNGC 3 cut(s) 273, 286, 552
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 3 cut(s) 194, 239, 267
HapII CCGG 2 cut(s) 264, 584
Hin1II CATG 5 cut(s) 74, 223, 349, 400, 469
HinfI GANTC 2 cut(s) 144, 478
HpaII CCGG 2 cut(s) 264, 584
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 3 cut(s) 325, 436, 455
Hpy188I TCNGA 2 cut(s) 96, 496
Hpy188III TCNNGA 1 cut(s) 482
Hpy8I GTNNAC 3 cut(s) 325, 436, 455
Hpy99I CGWCG 1 cut(s) 144
HpyAV CCTTC 2 cut(s) 160, 215
HpyCH4III ACNGT 4 cut(s) 61, 296, 322, 452
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 1 cut(s) 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 565
HpyF3I CTNAG 2 cut(s) 495, 660
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 5 cut(s) 74, 223, 349, 400, 469
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 4 cut(s) 107, 216, 224, 279
LmnI GCTCC 2 cut(s) 266, 329
LpnPI CCDG 9 cut(s) 133, 145, 176, 203, 277, 403, 495, 523, 597
Lsp1109I GCAGC 3 cut(s) 284, 297, 563
MaeI CTAG 2 cut(s) 44, 269
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 5 cut(s) 209, 401, 475, 512, 607
MalI GATC 4 cut(s) 109, 218, 226, 281
MbiI CCGCTC 1 cut(s) 310
MboI GATC 4 cut(s) 107, 216, 224, 279
MboII GAAGA 4 cut(s) 193, 313, 379, 676
MhlI GDGCHC 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 5 cut(s) 341, 378, 444, 631, 701
MluNI TGGCCA 1 cut(s) 239
MlyI GAGTC 1 cut(s) 472
MnlI CCTC 3 cut(s) 263, 395, 570
Mox20I TGGCCA 1 cut(s) 239
MscI TGGCCA 1 cut(s) 239
MseI TTAA 3 cut(s) 356, 447, 521
MslI CAYNNNNRTG 2 cut(s) 218, 397
Msp20I TGGCCA 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 135
MspI CCGG 2 cut(s) 264, 584
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 565
NdeII GATC 4 cut(s) 107, 216, 224, 279
NlaIII CATG 5 cut(s) 74, 223, 349, 400, 469
NlaIV GGNNCC 1 cut(s) 618
NmeAIII GCCGAG 1 cut(s) 134
NmuCI GTSAC 3 cut(s) 401, 475, 607
PfeI GAWTC 1 cut(s) 144
PinAI ACCGGT 1 cut(s) 583
PkrI GCNGC 3 cut(s) 274, 287, 553
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
Psp124BI GAGCTC 1 cut(s) 334
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 618
PspPI GGNCC 1 cut(s) 581
RsaI GTAC 2 cut(s) 39, 362
RsaNI GTAC 2 cut(s) 38, 361
RseI CAYNNNNRTG 2 cut(s) 218, 397
SacI GAGCTC 1 cut(s) 334
SaqAI TTAA 3 cut(s) 356, 447, 521
SatI GCNGC 3 cut(s) 273, 286, 552
Sau3AI GATC 4 cut(s) 107, 216, 224, 279
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 334
SetI ASST 5 cut(s) 334, 366, 422, 501, 542
SinI GGWCC 1 cut(s) 581
SmiMI CAYNNNNRTG 2 cut(s) 218, 397
Sse9I AATT 5 cut(s) 341, 378, 444, 631, 701
SsiI CCGC 3 cut(s) 135, 308, 530
SspMI CTAG 2 cut(s) 44, 269
SstI GAGCTC 1 cut(s) 334
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 4 cut(s) 61, 296, 322, 452
TaiI ACGT 1 cut(s) 366
TasI AATT 5 cut(s) 341, 378, 444, 631, 701
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 3 cut(s) 356, 447, 521
Tru9I TTAA 3 cut(s) 356, 447, 521
TscAI CASTG 5 cut(s) 301, 325, 532, 561, 664
TseFI GTSAC 3 cut(s) 401, 475, 607
TseI GCWGC 3 cut(s) 272, 285, 551
Tsp45I GTSAC 3 cut(s) 401, 475, 607
TspDTI ATGAA 6 cut(s) 179, 189, 334, 385, 412, 702
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 5 cut(s) 301, 325, 532, 561, 664
VpaK11BI GGWCC 1 cut(s) 581
XapI RAATTY 2 cut(s) 341, 701
XspI CTAG 2 cut(s) 44, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.