pycom08g10270

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015453
Physical Location & Seq
Reverse (-)
8633395 .. 8635725
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g10270.3

Sequence Viewer

Length: 870 bp
ATGGACAATATATCCCACCAAATTGGCGTATCGGGTTCTAAAAAAAAATTCAATGGAAAAAGAAAGCCAATAATTAAAAAGAAAAAAGCCAATAAAGAAACACAAGGGAAAAAGGAGGTTTGCGCAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGGAATGGAGAAAATGCTACTTGGATGTCATTCTTGTACCACTAGGGTTTCTCATAACTGTTTCTTATCATGCTTGGTTATGGCATAAAGTTCGGACTGACCCACGATCAACCATCATCGGCATAAACACCAGCGGACGACGATTCTGGGTCTCGGCAATGATGAAGGACAATGAAAAGAAGAACATCCTGGCCGTCCAAACCCTTCGTAACACGATCATGGGATCAACCCTAATGGCCACTACATCAATCCTCTTATGCTCCGGCCTAGCAGCCGTGATCAGCAGCACATACAGCGTGAAGAAACCGCTCAATGACACTGTTTACGGAGCTCACGGGGAATTCATGGTTGCTTTAAAATACGTTACCCTACTCACAATTTTCCTCTTCTCATTCATGTGTCACTCGCTTTCAATCAGGTTCATAAACCAAGTGAACATCCTAATTAACACTCCACAAGACCCCATGTCCTTGGTGACTCCAGAGTATGTGTCTGAGCTTTTAGAAAAGGGTTACTTGTTAAACACTGCGGGGAACAGGTTGTTTTATGCAGCACTGCCTCTTGTGCTATGGATATTTGGACCGGTTTTGGTGTTTTTGTGTTCTGTCACAATGGTTCCCGTGCTTTACAATTTGGACTTTGTGTTTAGTAGTAGCACTGAGAAGAGAAAAATGGATATTGGTAATGAAAATAGGGATGAATTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

290

Amino Acids

33.0

Weight (kDa)

9.53

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015453)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31330
fragaria_vesca FvH4_2g36420
malus_domestica MD08G1123900.v1.1 MD15G1102500.v1.1
prunus_persica Prupe.1G455600_v2.0.a1
pyrus_communis pycom08g10270
rosa_chinensis RchiOBHm_Chr6g0305481
rosa_laevigata RLG00000010888
rosa_multiflora Rmu_sc0007817.1_g000001
rosa_roxburghii Rroxscaffold_7G00162770
rosa_rugosa Rorug06G0340400
rosa_samantha Rh6AG452600 Rh6BG449200 Rh6CG466300 Rh6DG453000
rosa_wichuraiana Rw6G039440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 124
AccBSI CCGCTC 1 cut(s) 469
AciI CCGC 3 cut(s) 294, 467, 689
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 2 cut(s) 351, 396
AcsI RAATTY 3 cut(s) 47, 500, 860
AfaI GTAC 1 cut(s) 198
AgeI ACCGGT 1 cut(s) 742
AgsI TTSAA 2 cut(s) 52, 573
AhdI GACNNNNNGTC 1 cut(s) 625
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 2 cut(s) 491, 658
AluI AGCT 2 cut(s) 491, 658
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 1 cut(s) 316
AlwI GGATC 1 cut(s) 391
AoxI GGCC 3 cut(s) 351, 396, 424
ApeKI GCWGC 3 cut(s) 431, 444, 710
ApoI RAATTY 3 cut(s) 47, 500, 860
AsiGI ACCGGT 1 cut(s) 742
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 740
AsuHPI GGTGA 1 cut(s) 646
AvaII GGWCC 1 cut(s) 740
BalI TGGCCA 1 cut(s) 398
BanII GRGCYC 1 cut(s) 493
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 3 cut(s) 443, 456, 722
BccI CCATC 2 cut(s) 154, 281
BceAI ACGGC 2 cut(s) 338, 419
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 1 cut(s) 350
BclI TGATCA 1 cut(s) 438
BcoDI GTCTC 1 cut(s) 316
BfaI CTAG 2 cut(s) 203, 428
BisI GCNGC 3 cut(s) 432, 445, 711
BlsI GCNGC 3 cut(s) 433, 446, 712
Bme1390I CCNGG 1 cut(s) 350
Bme18I GGWCC 1 cut(s) 740
BmeRI GACNNNNNGTC 1 cut(s) 625
BmgT120I GGNCC 1 cut(s) 740
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 1 cut(s) 350
BpmI CTGGAG 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 630
BsaWI WCCGGW 1 cut(s) 742
Bse118I RCCGGY 1 cut(s) 742
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 630
BseGI GGATG 4 cut(s) 190, 345, 597, 862
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 2 cut(s) 645, 810
BseXI GCAGC 3 cut(s) 443, 456, 722
BshFI GGCC 3 cut(s) 353, 398, 426
BshTI ACCGGT 1 cut(s) 742
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 2 cut(s) 423, 743
BsmAI GTCTC 1 cut(s) 316
BsnI GGCC 3 cut(s) 353, 398, 426
Bso31I GGTCTC 1 cut(s) 316
Bsp1286I GDGCHC 1 cut(s) 493
Bsp143I GATC 4 cut(s) 266, 375, 383, 438
BspACI CCGC 3 cut(s) 294, 467, 689
BspANI GGCC 3 cut(s) 353, 398, 426
BspCNI CTCAG 2 cut(s) 646, 811
BspLI GGNNCC 1 cut(s) 777
BspPI GGATC 1 cut(s) 391
BspTNI GGTCTC 1 cut(s) 316
BsrBI CCGCTC 1 cut(s) 469
BsrDI GCAATG 1 cut(s) 324
BsrFI RCCGGY 1 cut(s) 742
BssAI RCCGGY 1 cut(s) 742
BssECI CCNNGG 1 cut(s) 630
BssMI GATC 4 cut(s) 266, 375, 383, 438
BssT1I CCWWGG 1 cut(s) 630
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 2 cut(s) 220, 481
Bst6I CTCTTC 2 cut(s) 551, 818
BstDEI CTNAG 2 cut(s) 654, 819
BstF5I GGATG 4 cut(s) 190, 345, 597, 862
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 4 cut(s) 269, 378, 386, 441
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 4 cut(s) 266, 375, 383, 438
BstMWI GCNNNNNNNGC 2 cut(s) 453, 724
BstNI CCWGG 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 348
BstV1I GCAGC 3 cut(s) 443, 456, 722
BstXI CCANNNNNNTGG 2 cut(s) 23, 631
BsuRI GGCC 3 cut(s) 353, 398, 426
BtsCI GGATG 4 cut(s) 190, 345, 597, 862
BtsI GCAGTG 2 cut(s) 684, 713
BtsIMutI CAGTG 4 cut(s) 477, 684, 713, 816
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 742
Cfr13I GGNCC 1 cut(s) 740
Csp6I GTAC 1 cut(s) 197
CspAI ACCGGT 1 cut(s) 742
CviAII CATG 5 cut(s) 230, 379, 505, 556, 625
CviJI RGCY 8 cut(s) 67, 89, 353, 398, 426, 434, 491, 658
CviKI_1 RGCY 8 cut(s) 67, 89, 353, 398, 426, 434, 491, 658
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 2 cut(s) 654, 819
DpnI GATC 4 cut(s) 268, 377, 385, 440
DpnII GATC 4 cut(s) 266, 375, 383, 438
DraI TTTAAA 1 cut(s) 516
DriI GACNNNNNGTC 1 cut(s) 625
EaeI YGGCCR 2 cut(s) 351, 396
Eam1104I CTCTTC 2 cut(s) 551, 818
Eam1105I GACNNNNNGTC 1 cut(s) 625
EarI CTCTTC 2 cut(s) 551, 818
Ecl136II GAGCTC 1 cut(s) 491
Eco130I CCWWGG 1 cut(s) 630
Eco24I GRGCYC 1 cut(s) 493
Eco31I GGTCTC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 740
Eco53kI GAGCTC 1 cut(s) 491
EcoICRI GAGCTC 1 cut(s) 491
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 1 cut(s) 630
EcoT38I GRGCYC 1 cut(s) 493
ErhI CCWWGG 1 cut(s) 630
FaeI CATG 5 cut(s) 233, 382, 508, 559, 628
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FatI CATG 5 cut(s) 229, 378, 504, 555, 624
FauI CCCGC 1 cut(s) 682
FbaI TGATCA 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 432, 445, 711
FokI GGATG 3 cut(s) 197, 332, 584
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 3 cut(s) 432, 445, 711
FspBI CTAG 2 cut(s) 203, 428
FspI TGCGCA 1 cut(s) 124
GlaI GCGC 1 cut(s) 124
GluI GCNGC 3 cut(s) 432, 445, 711
GsuI CTGGAG 1 cut(s) 624
HaeIII GGCC 3 cut(s) 353, 398, 426
HapII CCGG 2 cut(s) 423, 743
HhaI GCGC 1 cut(s) 125
Hin1II CATG 5 cut(s) 233, 382, 508, 559, 628
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HinfI GANTC 2 cut(s) 303, 637
HpaII CCGG 2 cut(s) 423, 743
HphI GGTGA 1 cut(s) 646
Hpy166II GTNNAC 2 cut(s) 484, 595
Hpy188I TCNGA 2 cut(s) 255, 655
Hpy188III TCNNGA 1 cut(s) 641
Hpy8I GTNNAC 2 cut(s) 484, 595
Hpy99I CGWCG 1 cut(s) 303
HpyAV CCTTC 2 cut(s) 319, 374
HpyCH4III ACNGT 2 cut(s) 220, 481
HpyCH4IV ACGT 1 cut(s) 522
HpyCH4V TGCA 1 cut(s) 710
HpyF10VI GCNNNNNNNGC 2 cut(s) 453, 724
HpyF3I CTNAG 2 cut(s) 654, 819
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 5 cut(s) 233, 382, 508, 559, 628
HspAI GCGC 1 cut(s) 123
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 4 cut(s) 266, 375, 383, 438
LmnI GCTCC 2 cut(s) 425, 488
LpnPI CCDG 9 cut(s) 292, 304, 335, 362, 436, 562, 654, 682, 756
Lsp1109I GCAGC 3 cut(s) 443, 456, 722
MaeI CTAG 2 cut(s) 203, 428
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 6 cut(s) 368, 523, 560, 634, 671, 766
MalI GATC 4 cut(s) 268, 377, 385, 440
MbiI CCGCTC 1 cut(s) 469
MboI GATC 4 cut(s) 266, 375, 383, 438
MboII GAAGA 4 cut(s) 352, 472, 538, 835
MhlI GDGCHC 1 cut(s) 493
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 8 cut(s) 21, 47, 72, 500, 537, 603, 790, 860
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 1 cut(s) 631
MnlI CCTC 4 cut(s) 109, 422, 554, 729
Mox20I TGGCCA 1 cut(s) 398
MscI TGGCCA 1 cut(s) 398
MseI TTAA 4 cut(s) 75, 515, 606, 680
MslI CAYNNNNRTG 2 cut(s) 377, 556
Msp20I TGGCCA 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 2 cut(s) 423, 743
MspR9I CCNGG 1 cut(s) 350
MvaI CCWGG 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 453, 724
NdeII GATC 4 cut(s) 266, 375, 383, 438
NlaIII CATG 5 cut(s) 233, 382, 508, 559, 628
NlaIV GGNNCC 1 cut(s) 777
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 3 cut(s) 560, 634, 766
NsbI TGCGCA 1 cut(s) 124
PfeI GAWTC 1 cut(s) 303
PinAI ACCGGT 1 cut(s) 742
PkrI GCNGC 3 cut(s) 433, 446, 712
PleI GAGTC 1 cut(s) 631
PpsI GAGTC 1 cut(s) 631
Psp124BI GAGCTC 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 1 cut(s) 740
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
RseI CAYNNNNRTG 2 cut(s) 377, 556
SacI GAGCTC 1 cut(s) 493
SaqAI TTAA 4 cut(s) 75, 515, 606, 680
SatI GCNGC 3 cut(s) 432, 445, 711
Sau3AI GATC 4 cut(s) 266, 375, 383, 438
Sau96I GGNCC 1 cut(s) 740
SchI GAGTC 1 cut(s) 631
ScrFI CCNGG 1 cut(s) 350
SduI GDGCHC 1 cut(s) 493
SetI ASST 6 cut(s) 120, 493, 525, 581, 660, 701
SinI GGWCC 1 cut(s) 740
SmiMI CAYNNNNRTG 2 cut(s) 377, 556
Sse9I AATT 8 cut(s) 21, 47, 72, 500, 537, 603, 790, 860
SsiI CCGC 3 cut(s) 294, 467, 689
SspMI CTAG 2 cut(s) 203, 428
SstI GAGCTC 1 cut(s) 493
StyD4I CCNGG 1 cut(s) 348
StyI CCWWGG 1 cut(s) 630
TaaI ACNGT 2 cut(s) 220, 481
TaiI ACGT 1 cut(s) 525
TasI AATT 8 cut(s) 21, 47, 72, 500, 537, 603, 790, 860
TfiI GAWTC 1 cut(s) 303
Tru1I TTAA 4 cut(s) 75, 515, 606, 680
Tru9I TTAA 4 cut(s) 75, 515, 606, 680
TscAI CASTG 4 cut(s) 484, 691, 720, 823
TseFI GTSAC 3 cut(s) 560, 634, 766
TseI GCWGC 3 cut(s) 431, 444, 710
Tsp45I GTSAC 3 cut(s) 560, 634, 766
TspDTI ATGAA 6 cut(s) 338, 348, 493, 544, 571, 861
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 4 cut(s) 484, 691, 720, 823
VpaK11BI GGWCC 1 cut(s) 740
XapI RAATTY 3 cut(s) 47, 500, 860
XspI CTAG 2 cut(s) 203, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.