MD15G1102500.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015453
Physical Location & Seq
Reverse (-)
7254485 .. 7256976
2492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1102500.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGAATGGAGAAAATGCTACTTGGATGTCATTCTTGTACCACTAGGGTTCCTCATGACTGCTTCGTATCATGCTTGGTTATGGCATAAGGTTCGGACCGACCCACGATCTACCATCATCGGCATAAACACCAGCGGACGACGATTCTGGGTCTCGGCGATGATGAAGGACAATGAAAAGAAGAACATCCTGGCCGTCCAAACCCTTCGTAACACGATCATGGGATCAACCCTAATGGCCACCACATCAATCCTCCTATGCTCCGGCCTAGCAGCTGTGATCAGCAGCACATACAGTGTGAAGAAACCGCTCAATGACACCGTTTACGGGGCACACGGGGAATTCATGGTTGCTTTAAAATACGTTACCCTACTCACAATTTTCCTCTTCTCGTTCATGTGTCACTCGCTTTCAATCAGGTTCATAAACCAAGTGAACATCCTAATTAACAGTCCACAAGAACCCACGTCGTTGGTGACTCCAGAGTATGTGTCTGCGCTTTTGGAAAGGGGTTACTTGTTAAACACTGCGGGGAACAGGTTGTTTTATGGAGCACTGCCTCTCGTGCTTTGGATATTTGGACCAGTTTTGGTGTTTTTGTGTTCTGCCACGATGGTGCCTGTGCTTTACAATTTGGACTTTGTGTCTAGCAGTAGCACTGAAAAGACAAAAATGGATATTGGTAATGAAAATGGGGATGCATTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

237

Amino Acids

26.4

Weight (kDa)

8.87

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 217 2.4e-76 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015453)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31330
fragaria_vesca FvH4_2g36420
malus_domestica MD08G1123900.v1.1 MD15G1102500.v1.1
prunus_persica Prupe.1G455600_v2.0.a1
pyrus_communis pycom08g10270
rosa_chinensis RchiOBHm_Chr6g0305481
rosa_laevigata RLG00000010888
rosa_multiflora Rmu_sc0007817.1_g000001
rosa_roxburghii Rroxscaffold_7G00162770
rosa_rugosa Rorug06G0340400
rosa_samantha Rh6AG452600 Rh6BG449200 Rh6CG466300 Rh6DG453000
rosa_wichuraiana Rw6G039440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 616
AccBSI CCGCTC 1 cut(s) 310
AciI CCGC 3 cut(s) 135, 308, 530
AclWI GGATC 1 cut(s) 232
AcoI YGGCCR 2 cut(s) 192, 237
AcsI RAATTY 1 cut(s) 341
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 1 cut(s) 327
AgsI TTSAA 1 cut(s) 414
AhdI GACNNNNNGTC 1 cut(s) 643
AjiI CACGTC 1 cut(s) 468
AjnI CCWGG 1 cut(s) 189
AleI CACNNNNGTG 1 cut(s) 614
AluBI AGCT 1 cut(s) 275
AluI AGCT 1 cut(s) 275
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 1 cut(s) 157
AlwI GGATC 1 cut(s) 232
AoxI GGCC 3 cut(s) 192, 237, 265
ApeKI GCWGC 2 cut(s) 272, 285
ApoI RAATTY 1 cut(s) 341
AspLEI GCGC 1 cut(s) 499
AspS9I GGNCC 2 cut(s) 96, 581
AsuHPI GGTGA 1 cut(s) 487
AvaII GGWCC 2 cut(s) 96, 581
BaeGI GKGCMC 1 cut(s) 334
BalI TGGCCA 1 cut(s) 239
BanI GGYRCC 1 cut(s) 616
BauI CACGAG 1 cut(s) 563
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 2 cut(s) 284, 297
BccI CCATC 2 cut(s) 122, 607
BceAI ACGGC 1 cut(s) 179
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 1 cut(s) 191
BclI TGATCA 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 157
BfaI CTAG 3 cut(s) 44, 269, 648
BisI GCNGC 2 cut(s) 273, 286
BlsI GCNGC 2 cut(s) 274, 287
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 2 cut(s) 96, 581
BmeRI GACNNNNNGTC 1 cut(s) 643
BmgBI CACGTC 1 cut(s) 468
BmgT120I GGNCC 2 cut(s) 96, 581
BmiI GGNNCC 2 cut(s) 50, 618
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 688
BpmI CTGGAG 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 157
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 584
BseBI CCWGG 1 cut(s) 191
BseGI GGATG 4 cut(s) 31, 186, 438, 703
BseLI CCNNNNNNNGG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 584
BseSI GKGCMC 1 cut(s) 334
BseXI GCAGC 2 cut(s) 284, 297
BshFI GGCC 3 cut(s) 194, 239, 267
BshNI GGYRCC 1 cut(s) 616
BsiHKAI GWGCWC 1 cut(s) 556
BsiSI CCGG 1 cut(s) 264
BslI CCNNNNNNNGG 1 cut(s) 327
BsmAI GTCTC 1 cut(s) 157
BsnI GGCC 3 cut(s) 194, 239, 267
Bso31I GGTCTC 1 cut(s) 157
Bsp1286I GDGCHC 2 cut(s) 334, 556
Bsp143I GATC 4 cut(s) 107, 216, 224, 279
BspACI CCGC 3 cut(s) 135, 308, 530
BspANI GGCC 3 cut(s) 194, 239, 267
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 2 cut(s) 50, 618
BspPI GGATC 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 616
BspTNI GGTCTC 1 cut(s) 157
BsrBI CCGCTC 1 cut(s) 310
BsrI ACTGG 1 cut(s) 584
BssMI GATC 4 cut(s) 107, 216, 224, 279
BssSI CACGAG 1 cut(s) 563
Bst2BI CACGAG 1 cut(s) 563
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 3 cut(s) 296, 322, 452
Bst6I CTCTTC 1 cut(s) 392
BstF5I GGATG 4 cut(s) 31, 186, 438, 703
BstHHI GCGC 1 cut(s) 499
BstKTI GATC 4 cut(s) 110, 219, 227, 282
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 4 cut(s) 107, 216, 224, 279
BstMWI GCNNNNNNNGC 1 cut(s) 565
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 284, 297
BstXI CCANNNNNNTGG 1 cut(s) 472
BsuRI GGCC 3 cut(s) 194, 239, 267
BtgZI GCGATG 1 cut(s) 173
BtrI CACGTC 1 cut(s) 468
BtsCI GGATG 4 cut(s) 31, 186, 438, 703
BtsI GCAGTG 2 cut(s) 525, 554
BtsIMutI CAGTG 4 cut(s) 301, 525, 554, 657
CciI TCATGA 1 cut(s) 54
CfoI GCGC 1 cut(s) 499
Cfr13I GGNCC 2 cut(s) 96, 581
CpoI CGGWCCG 1 cut(s) 96
Csp6I GTAC 1 cut(s) 38
CspI CGGWCCG 1 cut(s) 96
CviAII CATG 5 cut(s) 55, 71, 220, 346, 397
CviJI RGCY 4 cut(s) 194, 239, 267, 275
CviKI_1 RGCY 4 cut(s) 194, 239, 267, 275
CviQI GTAC 1 cut(s) 38
DpnI GATC 4 cut(s) 109, 218, 226, 281
DpnII GATC 4 cut(s) 107, 216, 224, 279
DraI TTTAAA 1 cut(s) 357
DriI GACNNNNNGTC 1 cut(s) 643
EaeI YGGCCR 2 cut(s) 192, 237
Eam1104I CTCTTC 1 cut(s) 392
Eam1105I GACNNNNNGTC 1 cut(s) 643
EarI CTCTTC 1 cut(s) 392
Eco31I GGTCTC 1 cut(s) 157
Eco47I GGWCC 2 cut(s) 96, 581
EcoRI GAATTC 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 189
EcoT22I ATGCAT 1 cut(s) 703
FaeI CATG 5 cut(s) 58, 74, 223, 349, 400
FatI CATG 5 cut(s) 54, 70, 219, 345, 396
FauI CCCGC 1 cut(s) 523
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 2 cut(s) 273, 286
FokI GGATG 3 cut(s) 38, 173, 425
Fsp4HI GCNGC 2 cut(s) 273, 286
FspBI CTAG 3 cut(s) 44, 269, 648
GlaI GCGC 1 cut(s) 498
GluI GCNGC 2 cut(s) 273, 286
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 3 cut(s) 194, 239, 267
HapII CCGG 1 cut(s) 264
HhaI GCGC 1 cut(s) 499
Hin1II CATG 5 cut(s) 58, 74, 223, 349, 400
Hin6I GCGC 1 cut(s) 497
HinP1I GCGC 1 cut(s) 497
HinfI GANTC 2 cut(s) 144, 478
HpaII CCGG 1 cut(s) 264
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 3 cut(s) 325, 436, 455
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 2 cut(s) 55, 482
Hpy8I GTNNAC 3 cut(s) 325, 436, 455
Hpy99I CGWCG 2 cut(s) 144, 472
HpyAV CCTTC 2 cut(s) 160, 215
HpyCH4III ACNGT 3 cut(s) 296, 322, 452
HpyCH4IV ACGT 2 cut(s) 363, 467
HpyCH4V TGCA 1 cut(s) 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 565
HpySE526I ACGT 2 cut(s) 363, 467
Hsp92II CATG 5 cut(s) 58, 74, 223, 349, 400
HspAI GCGC 1 cut(s) 497
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 4 cut(s) 107, 216, 224, 279
LmnI GCTCC 2 cut(s) 266, 551
Lsp1109I GCAGC 2 cut(s) 284, 297
LweI GCATC 1 cut(s) 688
MaeI CTAG 3 cut(s) 44, 269, 648
MaeII ACGT 2 cut(s) 363, 467
MaeIII GTNAC 5 cut(s) 209, 364, 401, 475, 512
MalI GATC 4 cut(s) 109, 218, 226, 281
MbiI CCGCTC 1 cut(s) 310
MboI GATC 4 cut(s) 107, 216, 224, 279
MboII GAAGA 3 cut(s) 193, 313, 379
MhlI GDGCHC 2 cut(s) 334, 556
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 4 cut(s) 341, 378, 444, 631
MluNI TGGCCA 1 cut(s) 239
MlyI GAGTC 1 cut(s) 472
MnlI CCTC 4 cut(s) 62, 263, 395, 570
Mox20I TGGCCA 1 cut(s) 239
Mph1103I ATGCAT 1 cut(s) 703
MscI TGGCCA 1 cut(s) 239
MseI TTAA 3 cut(s) 356, 447, 521
MslI CAYNNNNRTG 3 cut(s) 218, 614, 706
Msp20I TGGCCA 1 cut(s) 239
MspA1I CMGCKG 2 cut(s) 135, 275
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 565
NdeII GATC 4 cut(s) 107, 216, 224, 279
NlaIII CATG 5 cut(s) 58, 74, 223, 349, 400
NlaIV GGNNCC 2 cut(s) 50, 618
NmeAIII GCCGAG 1 cut(s) 134
NmuCI GTSAC 2 cut(s) 401, 475
NsiI ATGCAT 1 cut(s) 703
OliI CACNNNNGTG 1 cut(s) 614
PagI TCATGA 1 cut(s) 54
PfeI GAWTC 1 cut(s) 144
PkrI GCNGC 2 cut(s) 274, 287
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 50, 618
PspPI GGNCC 2 cut(s) 96, 581
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
RseI CAYNNNNRTG 3 cut(s) 218, 614, 706
Rsr2I CGGWCCG 1 cut(s) 96
RsrII CGGWCCG 1 cut(s) 96
SaqAI TTAA 3 cut(s) 356, 447, 521
SatI GCNGC 2 cut(s) 273, 286
Sau3AI GATC 4 cut(s) 107, 216, 224, 279
Sau96I GGNCC 2 cut(s) 96, 581
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 2 cut(s) 334, 556
SetI ASST 6 cut(s) 93, 277, 366, 422, 470, 542
SfaNI GCATC 1 cut(s) 688
SinI GGWCC 2 cut(s) 96, 581
SmiMI CAYNNNNRTG 3 cut(s) 218, 614, 706
Sse9I AATT 4 cut(s) 341, 378, 444, 631
SsiI CCGC 3 cut(s) 135, 308, 530
SspMI CTAG 3 cut(s) 44, 269, 648
StyD4I CCNGG 1 cut(s) 189
TaaI ACNGT 3 cut(s) 296, 322, 452
TaiI ACGT 2 cut(s) 366, 470
TaqII GACCGA 1 cut(s) 113
TasI AATT 4 cut(s) 341, 378, 444, 631
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 3 cut(s) 356, 447, 521
Tru9I TTAA 3 cut(s) 356, 447, 521
TscAI CASTG 4 cut(s) 301, 532, 561, 664
TseFI GTSAC 2 cut(s) 401, 475
TseI GCWGC 2 cut(s) 272, 285
Tsp45I GTSAC 2 cut(s) 401, 475
TspDTI ATGAA 6 cut(s) 179, 189, 334, 385, 412, 702
TspRI CASTG 4 cut(s) 301, 532, 561, 664
VpaK11BI GGWCC 2 cut(s) 96, 581
XapI RAATTY 1 cut(s) 341
XspI CTAG 3 cut(s) 44, 269, 648
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.