FvH4_2g36920

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001808
Physical Location & Seq
Reverse (-)
26956854 .. 26960162
3309 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36920.t4

Sequence Viewer

Length: 933 bp
ATGGCCTTTGAAGCTTCTAATGGAAGAGGGCTTCAGCCCAGTTTATTCAATGTGGATGATATCGAGTTAAACACAAATTGGGAAGATGTGGCTTGTCCTATATGTTTGGATTTCCCTCACAATGGTGTACTCCTCCAGTGCACATCGCACAATAATGGATGCCGTCCTTTTGTCTGTGACACAAACCACTTGCACTCAAATTGTTTGGACCGTTTCAAAAGTGCAAATGGTATGTCATCTCCTTCAAAATCCGATGCTAGTCTGACAAGCGACACTGAGCCAGTGGCATCAGAAGACAACTGCAGGCCAACTTGCCCGTTGTGTCGGGGTGAAGTGACTGGGTGGGTTGTTGTTGATAAGGCTCGTGTTCATCTAGATGTGAAGAAGCGTTGTTGCGAGGAGCAACGATGCACATTTACGGGTACATACTTGGAATTGCAAAAACATGCTGAACTGGAGCATCCTCATTCTGAACCATCAAAAATCGATCCTGCCCGGCAGCTTGATTGGGAAAACTTCCAACAGTCTTCTGAGATCATTGATGTTTTGAGCACTATACATTCAGAAGTCCCTCGGGGGGTTGTTTTAGGTGATTATGTGATTGAATATGGTGATGATGAGACTGGGGATGAGTTTGAGGACTTTCATGGGGATGAACGTAACTGGTGGACCTCCTGTATATTGTATCAGGTATTTGATAACTTGAGGAATTCTAGAAATAGAAGAAGATCAAGAGTTGGTGACAATAGAAGAGGAGGCCGCCGAGCAAGCTATGATGCAAATTCTGATGACGGTTCTGTGACATCTGTTGATTTCACAGACTATAGGGCAGATGAGATTGATGATGACTTTATGAGTTCAAGTGGTTCCTCCAGGGGTGGCTCAAGTCATCGCAGTTCCCGTAGACGGCGTTCTCGCTTCTATGACAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

311

Amino Acids

35.11

Weight (kDa)

5.05

Isoelectric Point (pI)

66.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 28 - 184 9.4e-63 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 904
AciI CCGC 1 cut(s) 760
AclWI GGATC 1 cut(s) 482
AcsI RAATTY 2 cut(s) 709, 781
AcuI CTGAAG 1 cut(s) 17
AfaI GTAC 2 cut(s) 129, 424
AfiI CCNNNNNNNGG 3 cut(s) 122, 577, 906
AgsI TTSAA 6 cut(s) 11, 49, 217, 246, 605, 861
AjnI CCWGG 1 cut(s) 872
AleI CACNNNNGTG 1 cut(s) 123
AluBI AGCT 3 cut(s) 14, 502, 771
AluI AGCT 3 cut(s) 14, 502, 771
Alw21I GWGCWC 2 cut(s) 143, 554
Alw26I GTCTC 1 cut(s) 614
Alw44I GTGCAC 1 cut(s) 139
AlwI GGATC 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 573
AoxI GGCC 3 cut(s) 3, 305, 757
ApaLI GTGCAC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 499
ApoI RAATTY 2 cut(s) 709, 781
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AspS9I GGNCC 2 cut(s) 208, 669
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 4 cut(s) 341, 602, 623, 752
AvaI CYCGRG 1 cut(s) 573
AvaII GGWCC 2 cut(s) 208, 669
BaeGI GKGCMC 1 cut(s) 143
BauI CACGAG 1 cut(s) 363
BbsI GAAGAC 2 cut(s) 300, 519
Bbv12I GWGCWC 2 cut(s) 143, 554
BbvI GCAGC 1 cut(s) 511
BccI CCATC 1 cut(s) 484
BceAI ACGGC 2 cut(s) 147, 923
BciT130I CCWGG 1 cut(s) 874
BcnI CCSGG 1 cut(s) 496
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 3 cut(s) 258, 374, 714
BfmI CTRYAG 2 cut(s) 301, 823
BisI GCNGC 2 cut(s) 500, 760
BlsI GCNGC 2 cut(s) 501, 761
Bme1390I CCNGG 2 cut(s) 496, 874
Bme18I GGWCC 2 cut(s) 208, 669
BmeT110I CYCGRG 1 cut(s) 573
BmgT120I GGNCC 2 cut(s) 208, 669
BmiI GGNNCC 1 cut(s) 868
BmrFI CCNGG 2 cut(s) 496, 874
BmrI ACTGGG 3 cut(s) 33, 348, 633
BmsI GCATC 6 cut(s) 149, 244, 296, 398, 469, 766
BmuI ACTGGG 3 cut(s) 33, 348, 633
BpiI GAAGAC 2 cut(s) 300, 519
BpmI CTGGAG 3 cut(s) 119, 476, 856
BpuEI CTTGAG 2 cut(s) 724, 868
BpuMI CCSGG 1 cut(s) 496
Bsa29I ATCGAT 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 572, 873
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 577, 906
Bse1I ACTGG 7 cut(s) 39, 136, 281, 343, 459, 628, 668
BseBI CCWGG 1 cut(s) 874
BseCI ATCGAT 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 572, 873
BseGI GGATG 5 cut(s) 61, 164, 460, 634, 658
BseLI CCNNNNNNNGG 3 cut(s) 122, 577, 906
BseMII CTCAG 2 cut(s) 267, 522
BseNI ACTGG 7 cut(s) 39, 136, 281, 343, 459, 628, 668
BseRI GAGGAG 3 cut(s) 122, 413, 768
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 1 cut(s) 511
BshFI GGCC 3 cut(s) 5, 307, 759
BshVI ATCGAT 1 cut(s) 486
BsiHKAI GWGCWC 2 cut(s) 143, 554
BsiHKCI CYCGRG 1 cut(s) 573
BsiSI CCGG 1 cut(s) 496
BslFI GGGAC 1 cut(s) 554
BslI CCNNNNNNNGG 3 cut(s) 122, 577, 906
BsmAI GTCTC 1 cut(s) 614
BsmFI GGGAC 1 cut(s) 554
BsnI GGCC 3 cut(s) 5, 307, 759
BsoBI CYCGRG 1 cut(s) 573
Bsp1286I GDGCHC 2 cut(s) 143, 554
Bsp143I GATC 3 cut(s) 487, 534, 728
BspACI CCGC 1 cut(s) 760
BspANI GGCC 3 cut(s) 5, 307, 759
BspCNI CTCAG 2 cut(s) 268, 523
BspDI ATCGAT 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 868
BspMAI CTGCAG 1 cut(s) 305
BspPI GGATC 1 cut(s) 482
BsrI ACTGG 7 cut(s) 39, 136, 281, 343, 459, 628, 668
BssECI CCNNGG 2 cut(s) 572, 873
BssMI GATC 3 cut(s) 487, 534, 728
BssSI CACGAG 1 cut(s) 363
Bst2BI CACGAG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 874
Bst4CI ACNGT 4 cut(s) 212, 525, 794, 929
Bst6I CTCTTC 2 cut(s) 19, 745
BstC8I GCNNGC 2 cut(s) 305, 769
BstDEI CTNAG 2 cut(s) 276, 531
BstF5I GGATG 5 cut(s) 61, 164, 460, 634, 658
BstKTI GATC 3 cut(s) 490, 537, 731
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 3 cut(s) 487, 534, 728
BstMWI GCNNNNNNNGC 2 cut(s) 11, 768
BstNI CCWGG 1 cut(s) 874
BstNSI RCATGY 1 cut(s) 449
BstSCI CCNGG 2 cut(s) 494, 872
BstSFI CTRYAG 2 cut(s) 301, 823
BstSLI GKGCMC 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 2 cut(s) 300, 519
Bsu15I ATCGAT 1 cut(s) 486
BsuRI GGCC 3 cut(s) 5, 307, 759
BsuTUI ATCGAT 1 cut(s) 486
BtgZI GCGATG 2 cut(s) 129, 875
BtsCI GGATG 5 cut(s) 61, 164, 460, 634, 658
BtsIMutI CAGTG 3 cut(s) 143, 273, 288
Cac8I GCNNGC 2 cut(s) 305, 769
Cfr13I GGNCC 2 cut(s) 208, 669
ClaI ATCGAT 1 cut(s) 486
Csp6I GTAC 2 cut(s) 128, 423
CviAII CATG 2 cut(s) 446, 647
CviQI GTAC 2 cut(s) 128, 423
DdeI CTNAG 2 cut(s) 276, 531
DpnI GATC 3 cut(s) 489, 536, 730
DpnII GATC 3 cut(s) 487, 534, 728
Eam1104I CTCTTC 2 cut(s) 19, 745
EarI CTCTTC 2 cut(s) 19, 745
Eco32I GATATC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 208, 669
Eco57I CTGAAG 1 cut(s) 17
Eco88I CYCGRG 1 cut(s) 573
EcoRI GAATTC 1 cut(s) 709
EcoRII CCWGG 1 cut(s) 872
EcoRV GATATC 1 cut(s) 61
FaeI CATG 2 cut(s) 449, 650
FaqI GGGAC 1 cut(s) 554
FatI CATG 2 cut(s) 445, 646
FblI GTMKAC 1 cut(s) 904
Fnu4HI GCNGC 2 cut(s) 500, 760
FokI GGATG 5 cut(s) 68, 171, 447, 641, 665
Fsp4HI GCNGC 2 cut(s) 500, 760
FspBI CTAG 3 cut(s) 258, 374, 714
GluI GCNGC 2 cut(s) 500, 760
GsuI CTGGAG 3 cut(s) 119, 476, 856
HaeIII GGCC 3 cut(s) 5, 307, 759
HapII CCGG 1 cut(s) 496
Hin1II CATG 2 cut(s) 449, 650
HindIII AAGCTT 1 cut(s) 12
HpaII CCGG 1 cut(s) 496
HphI GGTGA 4 cut(s) 341, 602, 623, 752
Hpy166II GTNNAC 4 cut(s) 128, 141, 669, 905
Hpy188I TCNGA 7 cut(s) 253, 264, 292, 472, 532, 565, 787
Hpy188III TCNNGA 3 cut(s) 374, 714, 732
Hpy8I GTNNAC 4 cut(s) 128, 141, 669, 905
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 4 cut(s) 212, 525, 794, 929
HpyCH4IV ACGT 1 cut(s) 658
HpyCH4V TGCA 7 cut(s) 141, 193, 224, 303, 411, 439, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 768
HpyF3I CTNAG 2 cut(s) 276, 531
HpySE526I ACGT 1 cut(s) 658
Hsp92II CATG 2 cut(s) 449, 650
Kzo9I GATC 3 cut(s) 487, 534, 728
LmnI GCTCC 2 cut(s) 400, 457
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 6 cut(s) 149, 244, 296, 398, 469, 766
MaeI CTAG 3 cut(s) 258, 374, 714
MaeII ACGT 1 cut(s) 658
MaeIII GTNAC 5 cut(s) 176, 334, 659, 740, 799
MalI GATC 3 cut(s) 489, 536, 730
MboI GATC 3 cut(s) 487, 534, 728
MboII GAAGA 8 cut(s) 36, 95, 305, 394, 519, 735, 738, 762
MhlI GDGCHC 2 cut(s) 143, 554
MluCI AATT 5 cut(s) 76, 199, 434, 709, 781
MmeI TCCRAC 1 cut(s) 544
MseI TTAA 1 cut(s) 68
MslI CAYNNNNRTG 4 cut(s) 123, 153, 375, 651
MspI CCGG 1 cut(s) 496
MspR9I CCNGG 2 cut(s) 496, 874
MvaI CCWGG 1 cut(s) 874
MwoI GCNNNNNNNGC 2 cut(s) 11, 768
NciI CCSGG 1 cut(s) 496
NdeII GATC 3 cut(s) 487, 534, 728
NlaIII CATG 2 cut(s) 449, 650
NlaIV GGNNCC 1 cut(s) 868
NmeAIII GCCGAG 1 cut(s) 788
NmuCI GTSAC 4 cut(s) 176, 334, 740, 799
NspI RCATGY 1 cut(s) 449
OliI CACNNNNGTG 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 898
PkrI GCNGC 2 cut(s) 501, 761
Psp6I CCWGG 1 cut(s) 872
PspGI CCWGG 1 cut(s) 872
PspN4I GGNNCC 1 cut(s) 868
PspPI GGNCC 2 cut(s) 208, 669
PsrI GAACNNNNNNTAC 2 cut(s) 895, 927
PstI CTGCAG 1 cut(s) 305
RsaI GTAC 2 cut(s) 129, 424
RsaNI GTAC 2 cut(s) 128, 423
RseI CAYNNNNRTG 4 cut(s) 123, 153, 375, 651
SaqAI TTAA 1 cut(s) 68
SatI GCNGC 2 cut(s) 500, 760
Sau3AI GATC 3 cut(s) 487, 534, 728
Sau96I GGNCC 2 cut(s) 208, 669
ScrFI CCNGG 2 cut(s) 496, 874
SduI GDGCHC 2 cut(s) 143, 554
SetI ASST 7 cut(s) 16, 504, 592, 661, 674, 693, 773
SfaNI GCATC 6 cut(s) 149, 244, 296, 398, 469, 766
SfcI CTRYAG 2 cut(s) 301, 823
SinI GGWCC 2 cut(s) 208, 669
SmiMI CAYNNNNRTG 4 cut(s) 123, 153, 375, 651
SmlI CTYRAG 2 cut(s) 703, 883
SmoI CTYRAG 2 cut(s) 703, 883
Sse9I AATT 5 cut(s) 76, 199, 434, 709, 781
SsiI CCGC 1 cut(s) 760
SspMI CTAG 3 cut(s) 258, 374, 714
StyD4I CCNGG 2 cut(s) 494, 872
TaaI ACNGT 4 cut(s) 212, 525, 794, 929
TaiI ACGT 1 cut(s) 661
TaqI TCGA 2 cut(s) 63, 486
TasI AATT 5 cut(s) 76, 199, 434, 709, 781
TatI WGTACW 1 cut(s) 127
TauI GCSGC 1 cut(s) 762
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 3 cut(s) 143, 280, 288
TseFI GTSAC 4 cut(s) 176, 334, 740, 799
TseI GCWGC 1 cut(s) 499
Tsp45I GTSAC 4 cut(s) 176, 334, 740, 799
TspDTI ATGAA 3 cut(s) 359, 635, 669
TspRI CASTG 3 cut(s) 143, 280, 288
VneI GTGCAC 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 208, 669
XapI RAATTY 2 cut(s) 709, 781
XbaI TCTAGA 2 cut(s) 373, 713
XceI RCATGY 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 904
XspI CTAG 3 cut(s) 258, 374, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.