MD15G1108900.v1.1

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001808
Physical Location & Seq
Reverse (-)
7592071 .. 7595497
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1108900.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGCCTTTGAAGCTAATCAAAGAGGGCTTCAACCCTGTTTAATCAATATGGATGATATTGAGTTAAATATAAACTGGGAAGACGTGAATTGTCCTATATGTTTGGACTTTCCTCACAACAGTGTACTACTTCAGTGCTCATCTTATGAGAAAGGGTGCCGTCCTTTTGTTTGTGACACAGACCACTTACACTCCAATTGTTTAGACCGCTTCAAAAGTGCATATGGTATGTCATCTCCTTCAAAACCAGTTGTGAATCCTGCGGAAAATACTGAGCCAAAGGCATCAGAAGATGATTGCAAGCCTACTTGTCCTTTGTGTAGGGGGGAAGTTACTGGGTGGGTTGTTGTTGACAAGGCTCGTGTACAACTCGATGTGAAGAAGCGTTGTTGTGAGGAGCATCGCTGTACATTTATGGGTTCGTATTTGGAATTACAAAAACATGCTCAGCTGGAGCACCCTCATTCTCGTCCTTCAAAGATTGATCCTGCTCGGCAGCTTGATTGGGAAAATTTCCAGCAGTCCTCCGAGATCATAGATGTTTTGAGCACTATACATTCAGAAGTCCCTCGCGGGGTGGTTTTAGGAGACTATGTGATTGAATATGGTGATGATGAGACTGGGGATGAGTTTGAGGACTTCCATGGGGATGAACGCAACTGGTGGACCTCCTGCATCTTATATCAGGTTTTCGATAACTTAAGGAATTCTAGAAACAGAAGAAGGTCAAGAGTTGGTGACACAAGAAGAGGAAGTCGCCGAGCAAGCTATGATAATTCAAATTCTGATGAAGGTTCTGTGACATCTGTTGAATTCCCAGAGTATAGGGCAGATGAGATGGATGATGAGTTTATGAGTACAACTGGCCCCTCCAGAGGTGGCTCTAGTTATCGCAGGTCTTTCCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

303

Amino Acids

34.59

Weight (kDa)

4.97

Isoelectric Point (pI)

58.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 27 - 183 2.1e-64 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 885
AccB1I GGYRCC 1 cut(s) 156
AccII CGCG 1 cut(s) 573
AciI CCGC 3 cut(s) 208, 263, 573
AclWI GGATC 1 cut(s) 479
AcsI RAATTY 4 cut(s) 511, 706, 781, 812
AcuI CTGAAG 1 cut(s) 116
AfaI GTAC 4 cut(s) 126, 366, 409, 859
AfiI CCNNNNNNNGG 2 cut(s) 574, 875
AflII CTTAAG 1 cut(s) 700
AgsI TTSAA 8 cut(s) 11, 32, 214, 243, 477, 602, 780, 812
AjiI CACGTC 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 120
AluBI AGCT 4 cut(s) 14, 451, 499, 768
AluI AGCT 4 cut(s) 14, 451, 499, 768
Alw21I GWGCWC 3 cut(s) 140, 459, 551
Alw26I GTCTC 2 cut(s) 582, 611
AlwI GGATC 1 cut(s) 479
AoxI GGCC 2 cut(s) 3, 865
ApeKI GCWGC 1 cut(s) 496
ApoI RAATTY 4 cut(s) 511, 706, 781, 812
AspS9I GGNCC 2 cut(s) 666, 866
AsuHPI GGTGA 2 cut(s) 620, 749
AvaII GGWCC 1 cut(s) 666
BanI GGYRCC 1 cut(s) 156
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 3 cut(s) 140, 459, 551
BbvI GCAGC 1 cut(s) 508
BccI CCATC 1 cut(s) 832
BceAI ACGGC 1 cut(s) 144
BcoDI GTCTC 2 cut(s) 582, 611
BfaI CTAG 2 cut(s) 711, 885
BfrI CTTAAG 1 cut(s) 700
BfuAI ACCTGC 1 cut(s) 885
BisI GCNGC 1 cut(s) 497
BlpI GCTNAGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 498
Bme18I GGWCC 1 cut(s) 666
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 666, 866
BmiI GGNNCC 2 cut(s) 158, 868
BmrI ACTGGG 3 cut(s) 85, 345, 630
BmsI GCATC 3 cut(s) 293, 409, 684
BmuI ACTGGG 3 cut(s) 85, 345, 630
BpiI GAAGAC 1 cut(s) 87
BpmI CTGGAG 2 cut(s) 473, 856
Bpu1102I GCTNAGC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 643
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 579, 609
Bsc4I CCNNNNNNNGG 2 cut(s) 574, 875
Bse1I ACTGG 6 cut(s) 80, 248, 340, 625, 665, 868
BseDI CCNNGG 1 cut(s) 643
BseGI GGATG 4 cut(s) 58, 631, 655, 847
BseLI CCNNNNNNNGG 2 cut(s) 574, 875
BseMII CTCAG 2 cut(s) 264, 461
BseNI ACTGG 6 cut(s) 80, 248, 340, 625, 665, 868
BseRI GAGGAG 1 cut(s) 410
BseXI GCAGC 1 cut(s) 508
Bsh1236I CGCG 1 cut(s) 573
BshFI GGCC 2 cut(s) 5, 867
BshNI GGYRCC 1 cut(s) 156
BsiHKAI GWGCWC 3 cut(s) 140, 459, 551
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 2 cut(s) 574, 875
BsmAI GTCTC 2 cut(s) 582, 611
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 2 cut(s) 5, 867
Bsp1286I GDGCHC 3 cut(s) 140, 459, 551
Bsp1407I TGTACA 2 cut(s) 364, 407
Bsp143I GATC 2 cut(s) 484, 531
Bsp1720I GCTNAGC 1 cut(s) 447
Bsp19I CCATGG 1 cut(s) 643
BspACI CCGC 3 cut(s) 208, 263, 573
BspANI GGCC 2 cut(s) 5, 867
BspCNI CTCAG 2 cut(s) 265, 460
BspFNI CGCG 1 cut(s) 573
BspLI GGNNCC 2 cut(s) 158, 868
BspMI ACCTGC 1 cut(s) 885
BspPI GGATC 1 cut(s) 479
BspT107I GGYRCC 1 cut(s) 156
BspTI CTTAAG 1 cut(s) 700
BsrGI TGTACA 2 cut(s) 364, 407
BsrI ACTGG 6 cut(s) 80, 248, 340, 625, 665, 868
BssECI CCNNGG 1 cut(s) 643
BssMI GATC 2 cut(s) 484, 531
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 643
Bst2BI CACGAG 1 cut(s) 360
Bst4CI ACNGT 1 cut(s) 122
Bst6I CTCTTC 1 cut(s) 742
BstAFI CTTAAG 1 cut(s) 700
BstAUI TGTACA 2 cut(s) 364, 407
BstC8I GCNNGC 2 cut(s) 302, 766
BstDEI CTNAG 2 cut(s) 273, 447
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 4 cut(s) 58, 631, 655, 847
BstFNI CGCG 1 cut(s) 573
BstKTI GATC 2 cut(s) 487, 534
BstMAI GTCTC 2 cut(s) 582, 611
BstMBI GATC 2 cut(s) 484, 531
BstMWI GCNNNNNNNGC 2 cut(s) 11, 765
BstNSI RCATGY 1 cut(s) 446
BstUI CGCG 1 cut(s) 573
BstV1I GCAGC 1 cut(s) 508
BstV2I GAAGAC 1 cut(s) 87
BsuRI GGCC 2 cut(s) 5, 867
BtgI CCRYGG 1 cut(s) 643
BtgZI GCGATG 1 cut(s) 386
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 4 cut(s) 58, 631, 655, 847
BtsIMutI CAGTG 2 cut(s) 127, 140
BveI ACCTGC 1 cut(s) 885
Cac8I GCNNGC 2 cut(s) 302, 766
Cfr13I GGNCC 2 cut(s) 666, 866
Csp6I GTAC 4 cut(s) 125, 365, 408, 858
CviAII CATG 2 cut(s) 443, 644
CviQI GTAC 4 cut(s) 125, 365, 408, 858
DdeI CTNAG 2 cut(s) 273, 447
DpnI GATC 2 cut(s) 486, 533
DpnII GATC 2 cut(s) 484, 531
Eam1104I CTCTTC 1 cut(s) 742
EarI CTCTTC 1 cut(s) 742
Eco130I CCWWGG 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 666
Eco57I CTGAAG 1 cut(s) 116
EcoRI GAATTC 2 cut(s) 706, 812
EcoT14I CCWWGG 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 643
FaeI CATG 2 cut(s) 446, 647
FaqI GGGAC 1 cut(s) 551
FatI CATG 2 cut(s) 442, 643
FauI CCCGC 1 cut(s) 566
FauNDI CATATG 1 cut(s) 223
Fnu4HI GCNGC 1 cut(s) 497
FokI GGATG 4 cut(s) 65, 638, 662, 854
Fsp4HI GCNGC 1 cut(s) 497
FspBI CTAG 2 cut(s) 711, 885
GluI GCNGC 1 cut(s) 497
GsuI CTGGAG 2 cut(s) 473, 856
HaeIII GGCC 2 cut(s) 5, 867
Hin1II CATG 2 cut(s) 446, 647
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 1 cut(s) 256
HphI GGTGA 2 cut(s) 620, 749
Hpy166II GTNNAC 4 cut(s) 125, 352, 365, 666
Hpy188I TCNGA 4 cut(s) 289, 529, 562, 787
Hpy188III TCNNGA 3 cut(s) 711, 729, 873
Hpy8I GTNNAC 4 cut(s) 125, 352, 365, 666
HpyAV CCTTC 4 cut(s) 249, 483, 717, 785
HpyCH4III ACNGT 1 cut(s) 122
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 221, 300, 675
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 765
HpyF3I CTNAG 2 cut(s) 273, 447
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 2 cut(s) 446, 647
Kzo9I GATC 2 cut(s) 484, 531
LmnI GCTCC 2 cut(s) 397, 454
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 3 cut(s) 293, 409, 684
MaeI CTAG 2 cut(s) 711, 885
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 4 cut(s) 173, 331, 737, 799
MalI GATC 2 cut(s) 486, 533
MboI GATC 2 cut(s) 484, 531
MboII GAAGA 5 cut(s) 92, 302, 391, 732, 759
MfeI CAATTG 1 cut(s) 196
MhlI GDGCHC 3 cut(s) 140, 459, 551
MluCI AATT 8 cut(s) 88, 196, 431, 511, 706, 775, 781, 812
MseI TTAA 3 cut(s) 41, 65, 701
MslI CAYNNNNRTG 2 cut(s) 120, 648
MspA1I CMGCKG 1 cut(s) 451
MspCI CTTAAG 1 cut(s) 700
MunI CAATTG 1 cut(s) 196
MvnI CGCG 1 cut(s) 573
MwoI GCNNNNNNNGC 2 cut(s) 11, 765
NcoI CCATGG 1 cut(s) 643
NdeI CATATG 1 cut(s) 223
NdeII GATC 2 cut(s) 484, 531
NlaIII CATG 2 cut(s) 446, 647
NlaIV GGNNCC 2 cut(s) 158, 868
NmeAIII GCCGAG 2 cut(s) 472, 785
NmuCI GTSAC 3 cut(s) 173, 737, 799
NspI RCATGY 1 cut(s) 446
OliI CACNNNNGTG 1 cut(s) 120
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 158, 868
PspPI GGNCC 2 cut(s) 666, 866
PvuII CAGCTG 1 cut(s) 451
RsaI GTAC 4 cut(s) 126, 366, 409, 859
RsaNI GTAC 4 cut(s) 125, 365, 408, 858
RseI CAYNNNNRTG 2 cut(s) 120, 648
SaqAI TTAA 3 cut(s) 41, 65, 701
SatI GCNGC 1 cut(s) 497
Sau3AI GATC 2 cut(s) 484, 531
Sau96I GGNCC 2 cut(s) 666, 866
SduI GDGCHC 3 cut(s) 140, 459, 551
SfaNI GCATC 3 cut(s) 293, 409, 684
SinI GGWCC 1 cut(s) 666
SmiMI CAYNNNNRTG 2 cut(s) 120, 648
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
Sse9I AATT 8 cut(s) 88, 196, 431, 511, 706, 775, 781, 812
SsiI CCGC 3 cut(s) 208, 263, 573
SspMI CTAG 2 cut(s) 711, 885
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 1 cut(s) 122
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 372, 693
TasI AATT 8 cut(s) 88, 196, 431, 511, 706, 775, 781, 812
TatI WGTACW 4 cut(s) 124, 364, 407, 857
TfiI GAWTC 1 cut(s) 256
Tru1I TTAA 3 cut(s) 41, 65, 701
Tru9I TTAA 3 cut(s) 41, 65, 701
TscAI CASTG 2 cut(s) 127, 140
TseFI GTSAC 3 cut(s) 173, 737, 799
TseI GCWGC 1 cut(s) 496
Tsp45I GTSAC 3 cut(s) 173, 737, 799
TspDTI ATGAA 2 cut(s) 666, 804
TspRI CASTG 2 cut(s) 127, 140
Vha464I CTTAAG 1 cut(s) 700
VpaK11BI GGWCC 1 cut(s) 666
XapI RAATTY 4 cut(s) 511, 706, 781, 812
XbaI TCTAGA 1 cut(s) 710
XceI RCATGY 1 cut(s) 446
XspI CTAG 2 cut(s) 711, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.