FvH4_2g38560

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012409
Physical Location & Seq
Reverse (-)
27862970 .. 27865536
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38560.t1

Sequence Viewer

Length: 1296 bp
ATGGCTCTTCTCACTTTCCAACCAGAGGCCACAGAGCCCAAGAAGAAGAAGAAACATGTCCAACCACAACAACAACCACCCTCTAAACGCAGCCACCATAAGCAGCACTCCTCCTCAAAAAAGCAGCAGCCACCTCCACAAAAGCCCCCCTCCACCTCTTCATGGGATCACTTCAAGAACCTCCTCACCTGCAAACAGATCGAGGGTTCCAGAGTCCACGACCCCTCCAAAAACGCCGTCGGCTACTCCAAGCTCGGGTCCTCCTGCGGCTCTATATGCAGTTTCAAAGACGTTGTTCACGGAAACACAACCAGCCGGGTTGTTCACAGAGCCGATAACTCGCCGGACAGTAGCTCGCTGGGTCAAGAAACCGGGTTGCTGAGCCGGAAAGTTGCATCCCGGTCTGAGTCAGGGTCAGCTTCTAGATCCAACCGCGGTGGCGGTGCTGGTTATACGTCATCTAATTCGTCTAGAGGGATGCAATTCAGGAAGCTCTCCGGGTGTTACGAGTGCCATTACATCGTTGACCCTAGCAGGTACCCAGTTCCAAGGTCTACTATCTGCTCTTGCCCTCATTGCGGTGAGATCTTCCCCAAATTGGAGAGTTTGGAACTTCACCAATCCATTCGCCATGCTGTATCGGAGCTAGGAGTGGAAGATTCCGGCAGAAACATCGTGGAGATAATATTCAAGTCGAGCTGGCTGAAGAAGGACAGTCCCATTTGCAGGATCGAACGGATACTCAAGGTCCAGAACACACAGCGCACGATCCAACGCTTCGAGGACTGCCGCGACGCCGTGAAGAACCGAGCCCTTAACAGCGCCCGAAAGAATCCCCGCTGCGCCGCCGACGGCAACGAGCTGCTGCGCTTCCACTGCACCAGCATCTCCTGCCCCCTCGGCGCGCGTGGCTCCACCAACCTCTGCGGCTCCGTCCCGGGCTGCGGTGTCTGCACCACCATCCGGCACGGCTTCCAAGGCCACGGAGGAGGAGAGGTTGTTGGCAGCAAGGGAGTGCGGACTACTGCCAGCAGCGGTAGGGCCCACGACTCGTTGCATTGTACGGACGGGCGTAGGGCAATGCTGGTTTGCCGCGTCATTGCCGGCAGGGTGAGGCGGGTTGCGGAAGATGCGGCGGAGGAGGAGGGTGTGACAGCATCATCATCAGCTGGTGGGACGTACGATTCCGTCGCCGGGTACGCCGGAGTGTTCTCGAATCTAGAGGAGCTGGTTGTGTTTAATCCGAGGGCGATACTCCCGTGTTTCGTAGTCATTTACAAAGCTCTTGACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

432

Amino Acids

46.72

Weight (kDa)

9.42

Isoelectric Point (pI)

57.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012409)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75710
fragaria_vesca FvH4_2g38560
malus_domestica MD08G1013600.v1.1 MD15G1013200.v1.1
prunus_persica Prupe.1G366300_v2.0.a1
pyrus_communis pycom08g01220 pycom15g01140
rosa_chinensis RchiOBHm_Chr6g0302741
rosa_laevigata RLG00000011142
rosa_multiflora Rmu_sc0009897.1_g000007 Rmu_sc0017617.1_g000001 Rmu_ssc0000460.1_g000001
rosa_roxburghii Rroxscaffold_7G00165360
rosa_rugosa Rorug06G0316700
rosa_samantha Rh6AG430000 Rh6BG472500 Rh6CG442500 Rh6DG429200
rosa_wichuraiana Rw6G037240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 2 cut(s) 197, 525
Acc65I GGTACC 1 cut(s) 537
AccB1I GGYRCC 1 cut(s) 537
AccI GTMKAC 1 cut(s) 554
AccII CGCG 5 cut(s) 435, 792, 905, 907, 1095
AclWI GGATC 4 cut(s) 174, 420, 737, 763
AcuI CTGAAG 1 cut(s) 725
AcyI GRCGYC 1 cut(s) 795
AfaI GTAC 4 cut(s) 539, 1063, 1181, 1199
AfiI CCNNNNNNNGG 9 cut(s) 25, 162, 255, 578, 598, 726, 944, 963, 1194
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 3 cut(s) 175, 286, 691
AlwI GGATC 4 cut(s) 174, 420, 737, 763
Ama87I CYCGRG 2 cut(s) 254, 937
AoxI GGCC 3 cut(s) 27, 979, 1041
ApaI GGGCCC 1 cut(s) 1045
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
Asp718I GGTACC 1 cut(s) 537
AspLEI GCGC 6 cut(s) 765, 824, 845, 870, 905, 907
AspS9I GGNCC 4 cut(s) 258, 748, 1041, 1042
AsuC2I CCSGG 7 cut(s) 317, 373, 400, 499, 938, 939, 1195
AsuHPI GGTGA 4 cut(s) 178, 593, 608, 1123
AvaI CYCGRG 2 cut(s) 254, 937
AvaII GGWCC 2 cut(s) 258, 748
BaeGI GKGCMC 1 cut(s) 1045
BaeI ACNNNNGTAYC 2 cut(s) 1244, 1277
BanI GGYRCC 1 cut(s) 537
BanII GRGCYC 3 cut(s) 39, 814, 1045
BccI CCATC 1 cut(s) 968
BceAI ACGGC 4 cut(s) 221, 782, 868, 985
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 655, 689
BciVI GTATCC 1 cut(s) 732
BcnI CCSGG 7 cut(s) 317, 373, 400, 499, 938, 939, 1195
BfaI CTAG 5 cut(s) 423, 471, 531, 647, 1220
BfoI RGCGCY 1 cut(s) 825
BfuAI ACCTGC 2 cut(s) 197, 525
BfuI GTATCC 1 cut(s) 732
BglI GCCNNNNNGGC 1 cut(s) 900
BglII AGATCT 1 cut(s) 585
BlpI GCTNAGC 1 cut(s) 380
Bme1390I CCNGG 7 cut(s) 317, 373, 400, 499, 938, 939, 1195
Bme18I GGWCC 2 cut(s) 258, 748
BmeT110I CYCGRG 2 cut(s) 254, 937
BmgT120I GGNCC 4 cut(s) 258, 748, 1041, 1042
BmiI GGNNCC 6 cut(s) 208, 259, 539, 913, 931, 1043
BmrFI CCNGG 7 cut(s) 317, 373, 400, 499, 938, 939, 1195
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 5 cut(s) 404, 468, 894, 1120, 1166
BmuI ACTGGG 1 cut(s) 536
Bpu1102I GCTNAGC 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 728
BpuMI CCSGG 7 cut(s) 317, 373, 400, 499, 938, 939, 1195
BsaHI GRCGYC 1 cut(s) 795
BsaJI CCNNGG 7 cut(s) 433, 548, 898, 937, 976, 982, 1244
BsaXI ACNNNNNCTCC 6 cut(s) 209, 239, 981, 984, 1011, 1014
Bsc4I CCNNNNNNNGG 9 cut(s) 25, 162, 255, 578, 598, 726, 944, 963, 1194
Bse118I RCCGGY 1 cut(s) 1103
Bse1I ACTGG 1 cut(s) 542
Bse3DI GCAATG 3 cut(s) 574, 1086, 1098
BseDI CCNNGG 7 cut(s) 433, 548, 898, 937, 976, 982, 1244
BseGI GGATG 3 cut(s) 395, 483, 960
BseLI CCNNNNNNNGG 9 cut(s) 25, 162, 255, 578, 598, 726, 944, 963, 1194
BseMI GCAATG 3 cut(s) 574, 1086, 1098
BseMII CTCAG 2 cut(s) 371, 396
BseNI ACTGG 1 cut(s) 542
BsePI GCGCGC 1 cut(s) 903
BseRI GAGGAG 8 cut(s) 100, 103, 173, 1002, 1005, 1154, 1157, 1238
BseSI GKGCMC 1 cut(s) 1045
BseYI CCCAGC 1 cut(s) 358
BsgI GTGCAG 2 cut(s) 862, 937
Bsh1236I CGCG 5 cut(s) 435, 792, 905, 907, 1095
BshFI GGCC 3 cut(s) 29, 981, 1043
BshNI GGYRCC 1 cut(s) 537
BsiHKCI CYCGRG 2 cut(s) 254, 937
BsiWI CGTACG 1 cut(s) 1179
BslFI GGGAC 3 cut(s) 702, 920, 1189
BslI CCNNNNNNNGG 9 cut(s) 25, 162, 255, 578, 598, 726, 944, 963, 1194
BsmFI GGGAC 3 cut(s) 702, 920, 1189
BsnI GGCC 3 cut(s) 29, 981, 1043
BsoBI CYCGRG 2 cut(s) 254, 937
Bsp120I GGGCCC 1 cut(s) 1041
Bsp1286I GDGCHC 3 cut(s) 39, 814, 1045
Bsp143I GATC 6 cut(s) 166, 198, 425, 585, 729, 768
Bsp1720I GCTNAGC 1 cut(s) 380
BspANI GGCC 3 cut(s) 29, 981, 1043
BspCNI CTCAG 2 cut(s) 372, 397
BspFNI CGCG 5 cut(s) 435, 792, 905, 907, 1095
BspLI GGNNCC 6 cut(s) 208, 259, 539, 913, 931, 1043
BspMI ACCTGC 2 cut(s) 197, 525
BspPI GGATC 4 cut(s) 174, 420, 737, 763
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 537
BsrDI GCAATG 3 cut(s) 574, 1086, 1098
BsrFI RCCGGY 1 cut(s) 1103
BsrI ACTGG 1 cut(s) 542
BssAI RCCGGY 1 cut(s) 1103
BssECI CCNNGG 7 cut(s) 433, 548, 898, 937, 976, 982, 1244
BssHII GCGCGC 1 cut(s) 903
BssMI GATC 6 cut(s) 166, 198, 425, 585, 729, 768
BssNI GRCGYC 1 cut(s) 795
BssT1I CCWWGG 2 cut(s) 548, 976
Bst4CI ACNGT 2 cut(s) 350, 716
Bst6I CTCTTC 2 cut(s) 12, 163
BstACI GRCGYC 1 cut(s) 795
BstAPI GCANNNNNTGC 1 cut(s) 891
BstC8I GCNNGC 5 cut(s) 356, 701, 905, 1030, 1105
BstDEI CTNAG 3 cut(s) 380, 405, 1293
BstDSI CCRYGG 2 cut(s) 433, 982
BstF5I GGATG 3 cut(s) 395, 483, 960
BstFNI CGCG 5 cut(s) 435, 792, 905, 907, 1095
BstH2I RGCGCY 1 cut(s) 825
BstHHI GCGC 6 cut(s) 765, 824, 845, 870, 905, 907
BstKTI GATC 6 cut(s) 169, 201, 428, 588, 732, 771
BstMBI GATC 6 cut(s) 166, 198, 425, 585, 729, 768
BstNSI RCATGY 1 cut(s) 59
BstSCI CCNGG 7 cut(s) 315, 371, 398, 497, 936, 937, 1193
BstSLI GKGCMC 1 cut(s) 1045
BstUI CGCG 5 cut(s) 435, 792, 905, 907, 1095
BstX2I RGATCY 2 cut(s) 425, 585
BstYI RGATCY 2 cut(s) 425, 585
BsuI GTATCC 1 cut(s) 732
BsuRI GGCC 3 cut(s) 29, 981, 1043
BtgI CCRYGG 2 cut(s) 433, 982
BtsCI GGATG 3 cut(s) 395, 483, 960
BtsI GCAGTG 1 cut(s) 874
BtsIMutI CAGTG 1 cut(s) 874
BveI ACCTGC 2 cut(s) 197, 525
Cac8I GCNNGC 5 cut(s) 356, 701, 905, 1030, 1105
CfoI GCGC 6 cut(s) 765, 824, 845, 870, 905, 907
Cfr10I RCCGGY 1 cut(s) 1103
Cfr13I GGNCC 4 cut(s) 258, 748, 1041, 1042
Cfr42I CCGCGG 1 cut(s) 436
Cfr9I CCCGGG 1 cut(s) 937
CseI GACGC 2 cut(s) 803, 1084
Csp6I GTAC 4 cut(s) 538, 1062, 1180, 1198
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CviAII CATG 3 cut(s) 56, 162, 632
CviQI GTAC 4 cut(s) 538, 1062, 1180, 1198
DdeI CTNAG 3 cut(s) 380, 405, 1293
DpnI GATC 6 cut(s) 168, 200, 427, 587, 731, 770
DpnII GATC 6 cut(s) 166, 198, 425, 585, 729, 768
Eam1104I CTCTTC 2 cut(s) 12, 163
EarI CTCTTC 2 cut(s) 12, 163
EciI GGCGGA 1 cut(s) 1151
Eco130I CCWWGG 2 cut(s) 548, 976
Eco24I GRGCYC 3 cut(s) 39, 814, 1045
Eco47I GGWCC 2 cut(s) 258, 748
Eco57I CTGAAG 1 cut(s) 725
Eco88I CYCGRG 2 cut(s) 254, 937
EcoO109I RGGNCCY 2 cut(s) 258, 1041
EcoT14I CCWWGG 2 cut(s) 548, 976
EcoT38I GRGCYC 3 cut(s) 39, 814, 1045
ErhI CCWWGG 2 cut(s) 548, 976
FaeI CATG 3 cut(s) 59, 165, 635
FaiI YATR 7 cut(s) 57, 99, 163, 275, 277, 453, 633
FaqI GGGAC 3 cut(s) 702, 920, 1189
FatI CATG 3 cut(s) 55, 161, 631
FauI CCCGC 2 cut(s) 845, 1110
FblI GTMKAC 1 cut(s) 554
FokI GGATG 3 cut(s) 382, 490, 947
FriOI GRGCYC 3 cut(s) 39, 814, 1045
FspBI CTAG 5 cut(s) 423, 471, 531, 647, 1220
GlaI GCGC 6 cut(s) 764, 823, 844, 869, 904, 906
GsaI CCCAGC 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 825
HaeIII GGCC 3 cut(s) 29, 981, 1043
HgaI GACGC 2 cut(s) 803, 1084
HhaI GCGC 6 cut(s) 765, 824, 845, 870, 905, 907
Hin1I GRCGYC 1 cut(s) 795
Hin1II CATG 3 cut(s) 59, 165, 635
Hin6I GCGC 6 cut(s) 763, 822, 843, 868, 903, 905
HinP1I GCGC 6 cut(s) 763, 822, 843, 868, 903, 905
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 8 cut(s) 213, 407, 659, 832, 1049, 1184, 1216, 1289
HphI GGTGA 4 cut(s) 178, 593, 608, 1123
Hpy166II GTNNAC 5 cut(s) 217, 298, 325, 526, 555
Hpy188I TCNGA 3 cut(s) 406, 643, 1245
Hpy8I GTNNAC 5 cut(s) 217, 298, 325, 526, 555
Hpy99I CGWCG 4 cut(s) 242, 797, 854, 1193
HpyAV CCTTC 1 cut(s) 703
HpyCH4III ACNGT 2 cut(s) 350, 716
HpyCH4IV ACGT 3 cut(s) 291, 455, 1178
HpyCH4V TGCA 8 cut(s) 192, 279, 395, 481, 726, 879, 954, 1057
HpyF3I CTNAG 3 cut(s) 380, 405, 1293
HpySE526I ACGT 3 cut(s) 291, 455, 1178
Hsp92I GRCGYC 1 cut(s) 795
Hsp92II CATG 3 cut(s) 59, 165, 635
HspAI GCGC 6 cut(s) 763, 822, 843, 868, 903, 905
KpnI GGTACC 1 cut(s) 541
KroI GCCGGC 1 cut(s) 1103
KroNI GCCGGC 1 cut(s) 1105
KspI CCGCGG 1 cut(s) 436
Kzo9I GATC 6 cut(s) 166, 198, 425, 585, 729, 768
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 4 cut(s) 643, 917, 935, 1225
LweI GCATC 5 cut(s) 404, 468, 894, 1120, 1166
MaeI CTAG 5 cut(s) 423, 471, 531, 647, 1220
MaeII ACGT 3 cut(s) 291, 455, 1178
MaeIII GTNAC 2 cut(s) 503, 1150
MalI GATC 6 cut(s) 168, 200, 427, 587, 731, 770
MboI GATC 6 cut(s) 166, 198, 425, 585, 729, 768
MboII GAAGA 9 cut(s) 55, 58, 61, 150, 580, 668, 718, 814, 1139
MflI RGATCY 2 cut(s) 425, 585
MhlI GDGCHC 3 cut(s) 39, 814, 1045
MluCI AATT 3 cut(s) 463, 482, 596
MlyI GAGTC 4 cut(s) 222, 416, 1043, 1283
MmeI TCCRAC 4 cut(s) 43, 85, 453, 796
MroNI GCCGGC 1 cut(s) 1103
MseI TTAA 2 cut(s) 816, 1239
MslI CAYNNNNRTG 1 cut(s) 579
MspA1I CMGCKG 4 cut(s) 435, 840, 1035, 1169
MspR9I CCNGG 7 cut(s) 317, 373, 400, 499, 938, 939, 1195
MvnI CGCG 5 cut(s) 435, 792, 905, 907, 1095
NaeI GCCGGC 1 cut(s) 1105
NciI CCSGG 7 cut(s) 317, 373, 400, 499, 938, 939, 1195
NdeII GATC 6 cut(s) 166, 198, 425, 585, 729, 768
NgoMIV GCCGGC 1 cut(s) 1103
NlaIII CATG 3 cut(s) 59, 165, 635
NlaIV GGNNCC 6 cut(s) 208, 259, 539, 913, 931, 1043
NmeAIII GCCGAG 1 cut(s) 879
NmuCI GTSAC 1 cut(s) 1150
NspI RCATGY 1 cut(s) 59
PaqCI CACCTGC 1 cut(s) 197
PauI GCGCGC 1 cut(s) 903
PciI ACATGT 1 cut(s) 55
PciSI GCTCTTC 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 1105
PfeI GAWTC 4 cut(s) 659, 832, 1184, 1216
Pfl23II CGTACG 1 cut(s) 1179
PleI GAGTC 4 cut(s) 221, 415, 1043, 1283
PpsI GAGTC 4 cut(s) 221, 415, 1043, 1283
PpuMI RGGWCCY 1 cut(s) 258
PscI ACATGT 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 258
PspFI CCCAGC 1 cut(s) 358
PspLI CGTACG 1 cut(s) 1179
PspN4I GGNNCC 6 cut(s) 208, 259, 539, 913, 931, 1043
PspOMI GGGCCC 1 cut(s) 1041
PspPI GGNCC 4 cut(s) 258, 748, 1041, 1042
PspPPI RGGWCCY 1 cut(s) 258
PsuI RGATCY 2 cut(s) 425, 585
PteI GCGCGC 1 cut(s) 903
PvuII CAGCTG 1 cut(s) 1169
RsaI GTAC 4 cut(s) 539, 1063, 1181, 1199
RsaNI GTAC 4 cut(s) 538, 1062, 1180, 1198
RseI CAYNNNNRTG 1 cut(s) 579
SacII CCGCGG 1 cut(s) 436
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 816, 1239
Sau3AI GATC 6 cut(s) 166, 198, 425, 585, 729, 768
Sau96I GGNCC 4 cut(s) 258, 748, 1041, 1042
SchI GAGTC 4 cut(s) 222, 416, 1043, 1283
ScrFI CCNGG 7 cut(s) 317, 373, 400, 499, 938, 939, 1195
SduI GDGCHC 3 cut(s) 39, 814, 1045
SfaNI GCATC 5 cut(s) 404, 468, 894, 1120, 1166
Sfr303I CCGCGG 1 cut(s) 436
SgrBI CCGCGG 1 cut(s) 436
SinI GGWCC 2 cut(s) 258, 748
SmaI CCCGGG 1 cut(s) 939
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 1 cut(s) 743
SmoI CTYRAG 1 cut(s) 743
Sse9I AATT 3 cut(s) 463, 482, 596
SspI AATATT 1 cut(s) 687
SspMI CTAG 5 cut(s) 423, 471, 531, 647, 1220
StyD4I CCNGG 7 cut(s) 315, 371, 398, 497, 936, 937, 1193
StyI CCWWGG 2 cut(s) 548, 976
TaaI ACNGT 2 cut(s) 350, 716
TaiI ACGT 3 cut(s) 294, 458, 1181
TaqI TCGA 5 cut(s) 201, 695, 732, 780, 1214
TasI AATT 3 cut(s) 463, 482, 596
TauI GCSGC 6 cut(s) 270, 792, 848, 930, 1095, 1136
TfiI GAWTC 4 cut(s) 659, 832, 1184, 1216
Tru1I TTAA 2 cut(s) 816, 1239
Tru9I TTAA 2 cut(s) 816, 1239
TscAI CASTG 1 cut(s) 881
TseFI GTSAC 1 cut(s) 1150
Tsp45I GTSAC 1 cut(s) 1150
TspDTI ATGAA 1 cut(s) 150
TspGWI ACGGA 6 cut(s) 315, 751, 922, 999, 1079, 1177
TspMI CCCGGG 1 cut(s) 937
TspRI CASTG 1 cut(s) 881
VpaK11BI GGWCC 2 cut(s) 258, 748
XbaI TCTAGA 3 cut(s) 422, 470, 1219
XceI RCATGY 1 cut(s) 59
XmaI CCCGGG 1 cut(s) 937
XmiI GTMKAC 1 cut(s) 554
XspI CTAG 5 cut(s) 423, 471, 531, 647, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.