pycom08g01220

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012409
Physical Location & Seq
Reverse (-)
904191 .. 906395
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01220.3

Sequence Viewer

Length: 789 bp
ATGGCTCTCTTCCCTTTTGCACCAGAGGCCACAGAGCCCAAGAAGAAAGAAACGCCACCATCAAAACGCAGGCAAAAGCAGTCGTCTTCAACCAACAAAAAGCAGCAAAAACCACCGTCCTCCTCCTCCTCCTCCTCCTCATGGGACCACATCAAGAACCTTCTGACCTGCAAACAGATCGAAGGGTCTAGAGTACACGACCCATCGAAAAATAATATTGTCGGTGGCGCAGCTGCCGAACACGGTGGAGGCTACTCGAAGCTGGGGTCATCCTGCAGCTCCATATGCAGCTTCAGAGATGTCGTTCATGGGAACACTAGGGTCGTTCACAGAGGTGACAGCTCGTCGCCCGAGAGTAGCACGCTGGGTCAGGAAACCGGGTTACTGGCTCGGAAACCTGCAACTCGGTCAATCAGATCCAACGGTTCTGGTTCTTCTGCTGCTTACCAGACGTCATCATCCTCCAGAGGAATGCAATTCCGGAAGCTCTCTGGGTGTTACGAGTGCCACATGATCGTTGACCCTAGCAGGTACCCAGTTCCTAGGTCTACTATTTGTGCTTGCTCTCAGTGTGGAGAGATCTATCCCAAAGATGGAGAGTTTGGAGCATCACCAAGCAATTCGCCATGCTGTGTCGGAGCTGGGCGTCGAAGATTCCGGCCGAAATATCGTGGAAATAATCTTCAAATCGAGCTGGCTCAAGAAAGACATCCCCTTCTGCAAGATCGAACGCATACTAAAGGTACAGAACACGCAGCGTACGATTCAACGGTTCGAGGACTGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

28.36

Weight (kDa)

9.67

Isoelectric Point (pI)

75.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012409)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75710
fragaria_vesca FvH4_2g38560
malus_domestica MD08G1013600.v1.1 MD15G1013200.v1.1
prunus_persica Prupe.1G366300_v2.0.a1
pyrus_communis pycom08g01220 pycom15g01140
rosa_chinensis RchiOBHm_Chr6g0302741
rosa_laevigata RLG00000011142
rosa_multiflora Rmu_sc0009897.1_g000007 Rmu_sc0017617.1_g000001 Rmu_ssc0000460.1_g000001
rosa_roxburghii Rroxscaffold_7G00165360
rosa_rugosa Rorug06G0316700
rosa_samantha Rh6AG430000 Rh6BG472500 Rh6CG442500 Rh6DG429200
rosa_wichuraiana Rw6G037240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 455
Acc36I ACCTGC 3 cut(s) 176, 406, 519
Acc65I GGTACC 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 531
AccI GTMKAC 1 cut(s) 548
AccIII TCCGGA 1 cut(s) 480
AclWI GGATC 1 cut(s) 411
AcoI YGGCCR 1 cut(s) 659
AcuI CTGAAG 1 cut(s) 277
AcyI GRCGYC 2 cut(s) 452, 646
AfaI GTAC 4 cut(s) 195, 533, 745, 761
AfiI CCNNNNNNNGG 2 cut(s) 141, 593
AgsI TTSAA 3 cut(s) 90, 686, 768
AhdI GACNNNNNGTC 1 cut(s) 343
AleI CACNNNNGTG 1 cut(s) 333
AluBI AGCT 8 cut(s) 233, 262, 279, 291, 342, 487, 641, 694
AluI AGCT 8 cut(s) 233, 262, 279, 291, 342, 487, 641, 694
AlwI GGATC 1 cut(s) 411
Ama87I CYCGRG 1 cut(s) 350
Aor13HI TCCGGA 1 cut(s) 480
AoxI GGCC 2 cut(s) 27, 659
ApeKI GCWGC 7 cut(s) 103, 230, 233, 276, 288, 440, 755
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
Asp718I GGTACC 1 cut(s) 531
AspA2I CCTAGG 1 cut(s) 542
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 145
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 2 cut(s) 347, 603
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 1 cut(s) 145
AvrII CCTAGG 1 cut(s) 542
BanI GGYRCC 1 cut(s) 531
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 78
BbvI GCAGC 7 cut(s) 115, 220, 242, 288, 300, 427, 767
BccI CCATC 3 cut(s) 67, 211, 587
BceAI ACGGC 1 cut(s) 769
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BcnI CCSGG 1 cut(s) 379
BfaI CTAG 4 cut(s) 189, 318, 525, 543
BfmI CTRYAG 1 cut(s) 274
BfuAI ACCTGC 3 cut(s) 176, 406, 519
BglII AGATCT 1 cut(s) 579
BisI GCNGC 7 cut(s) 104, 231, 234, 277, 289, 441, 756
BlnI CCTAGG 1 cut(s) 542
BlsI GCNGC 7 cut(s) 105, 232, 235, 278, 290, 442, 757
Bme1390I CCNGG 1 cut(s) 379
Bme18I GGWCC 1 cut(s) 145
BmeRI GACNNNNNGTC 1 cut(s) 343
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 2 cut(s) 146, 533
BmrFI CCNGG 1 cut(s) 379
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 1 cut(s) 617
BmuI ACTGGG 1 cut(s) 530
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 448
BpuEI CTTGAG 1 cut(s) 684
BpuMI CCSGG 1 cut(s) 379
BsaHI GRCGYC 2 cut(s) 452, 646
BsaJI CCNNGG 1 cut(s) 542
BsaWI WCCGGW 1 cut(s) 480
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 593
Bse1I ACTGG 2 cut(s) 390, 536
BseAI TCCGGA 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 542
BseGI GGATG 3 cut(s) 269, 458, 709
BseLI CCNNNNNNNGG 2 cut(s) 141, 593
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 2 cut(s) 390, 536
BseRI GAGGAG 6 cut(s) 112, 115, 118, 121, 124, 127
BseX3I CGGCCG 1 cut(s) 659
BseXI GCAGC 7 cut(s) 115, 220, 242, 288, 300, 427, 767
BseYI CCCAGC 3 cut(s) 262, 364, 641
Bsh1285I CGRYCG 1 cut(s) 662
BshFI GGCC 2 cut(s) 29, 661
BshNI GGYRCC 1 cut(s) 531
BsiEI CGRYCG 1 cut(s) 662
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 3 cut(s) 378, 481, 658
BsiWI CGTACG 1 cut(s) 759
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 2 cut(s) 141, 593
BsmFI GGGAC 1 cut(s) 158
BsmI GAATGC 1 cut(s) 477
BsnI GGCC 2 cut(s) 29, 661
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 39
Bsp13I TCCGGA 1 cut(s) 480
Bsp143I GATC 5 cut(s) 177, 416, 513, 579, 724
BspANI GGCC 2 cut(s) 29, 661
BspCNI CTCAG 1 cut(s) 580
BspEI TCCGGA 1 cut(s) 480
BspLI GGNNCC 2 cut(s) 146, 533
BspMAI CTGCAG 1 cut(s) 278
BspMI ACCTGC 3 cut(s) 176, 406, 519
BspPI GGATC 1 cut(s) 411
BspT107I GGYRCC 1 cut(s) 531
BsrI ACTGG 2 cut(s) 390, 536
BssECI CCNNGG 1 cut(s) 542
BssMI GATC 5 cut(s) 177, 416, 513, 579, 724
BssNI GRCGYC 2 cut(s) 452, 646
BssT1I CCWWGG 1 cut(s) 542
Bst4CI ACNGT 4 cut(s) 117, 245, 425, 772
Bst6I CTCTTC 1 cut(s) 14
BstACI GRCGYC 2 cut(s) 452, 646
BstC8I GCNNGC 4 cut(s) 71, 362, 562, 696
BstDEI CTNAG 1 cut(s) 567
BstF5I GGATG 3 cut(s) 269, 458, 709
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 5 cut(s) 180, 419, 516, 582, 727
BstMBI GATC 5 cut(s) 177, 416, 513, 579, 724
BstMCI CGRYCG 1 cut(s) 662
BstMWI GCNNNNNNNGC 2 cut(s) 26, 285
BstSCI CCNGG 1 cut(s) 377
BstSFI CTRYAG 1 cut(s) 274
BstV1I GCAGC 7 cut(s) 115, 220, 242, 288, 300, 427, 767
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 416, 579
BstYI RGATCY 2 cut(s) 416, 579
BstZI CGGCCG 1 cut(s) 659
BsuRI GGCC 2 cut(s) 29, 661
BtsCI GGATG 3 cut(s) 269, 458, 709
BtsIMutI CAGTG 1 cut(s) 575
BveI ACCTGC 3 cut(s) 176, 406, 519
Cac8I GCNNGC 4 cut(s) 71, 362, 562, 696
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 145
CseI GACGC 1 cut(s) 635
Csp6I GTAC 4 cut(s) 194, 532, 744, 760
CviAII CATG 4 cut(s) 141, 308, 511, 627
CviQI GTAC 4 cut(s) 194, 532, 744, 760
DdeI CTNAG 1 cut(s) 567
DpnI GATC 5 cut(s) 179, 418, 515, 581, 726
DpnII GATC 5 cut(s) 177, 416, 513, 579, 724
DriI GACNNNNNGTC 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 659
EagI CGGCCG 1 cut(s) 659
Eam1104I CTCTTC 1 cut(s) 14
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 1 cut(s) 14
EclXI CGGCCG 1 cut(s) 659
Eco130I CCWWGG 1 cut(s) 542
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 145
Eco52I CGGCCG 1 cut(s) 659
Eco57I CTGAAG 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 542
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 4 cut(s) 144, 311, 514, 630
FaiI YATR 7 cut(s) 142, 284, 286, 309, 512, 628, 735
FaqI GGGAC 1 cut(s) 158
FatI CATG 4 cut(s) 140, 307, 510, 626
FauNDI CATATG 1 cut(s) 284
FblI GTMKAC 1 cut(s) 548
Fnu4HI GCNGC 7 cut(s) 104, 231, 234, 277, 289, 441, 756
FokI GGATG 3 cut(s) 256, 445, 696
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 7 cut(s) 104, 231, 234, 277, 289, 441, 756
FspBI CTAG 4 cut(s) 189, 318, 525, 543
GlaI GCGC 1 cut(s) 229
GluI GCNGC 7 cut(s) 104, 231, 234, 277, 289, 441, 756
GsaI CCCAGC 3 cut(s) 266, 368, 645
GsuI CTGGAG 1 cut(s) 448
HaeIII GGCC 2 cut(s) 29, 661
HapII CCGG 3 cut(s) 378, 481, 658
HgaI GACGC 1 cut(s) 635
HhaI GCGC 1 cut(s) 230
Hin1I GRCGYC 2 cut(s) 452, 646
Hin1II CATG 4 cut(s) 144, 311, 514, 630
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 2 cut(s) 654, 764
HpaII CCGG 3 cut(s) 378, 481, 658
HphI GGTGA 2 cut(s) 347, 603
Hpy166II GTNNAC 4 cut(s) 196, 328, 520, 549
Hpy188I TCNGA 5 cut(s) 165, 296, 393, 416, 638
Hpy188III TCNNGA 6 cut(s) 154, 189, 371, 465, 481, 701
Hpy8I GTNNAC 4 cut(s) 196, 328, 520, 549
Hpy99I CGWCG 2 cut(s) 349, 651
HpyAV CCTTC 3 cut(s) 170, 176, 725
HpyCH4III ACNGT 4 cut(s) 117, 245, 425, 772
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 7 cut(s) 20, 171, 276, 288, 401, 475, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 285
HpyF3I CTNAG 1 cut(s) 567
HpySE526I ACGT 1 cut(s) 452
Hsp92I GRCGYC 2 cut(s) 452, 646
Hsp92II CATG 4 cut(s) 144, 311, 514, 630
HspAI GCGC 1 cut(s) 228
Kpn2I TCCGGA 1 cut(s) 480
KpnI GGTACC 1 cut(s) 535
Kzo9I GATC 5 cut(s) 177, 416, 513, 579, 724
LmnI GCTCC 3 cut(s) 284, 605, 638
Lsp1109I GCAGC 7 cut(s) 115, 220, 242, 288, 300, 427, 767
LweI GCATC 1 cut(s) 617
MaeI CTAG 4 cut(s) 189, 318, 525, 543
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 3 cut(s) 335, 381, 497
MalI GATC 5 cut(s) 179, 418, 515, 581, 726
MboI GATC 5 cut(s) 177, 416, 513, 579, 724
MboII GAAGA 5 cut(s) 55, 78, 426, 663, 674
MflI RGATCY 2 cut(s) 416, 579
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 2 cut(s) 476, 619
MmeI TCCRAC 2 cut(s) 444, 616
MroI TCCGGA 1 cut(s) 480
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 3 cut(s) 378, 481, 658
MspR9I CCNGG 1 cut(s) 379
Mva1269I GAATGC 1 cut(s) 477
MwoI GCNNNNNNNGC 2 cut(s) 26, 285
NciI CCSGG 1 cut(s) 379
NdeI CATATG 1 cut(s) 284
NdeII GATC 5 cut(s) 177, 416, 513, 579, 724
NlaIII CATG 4 cut(s) 144, 311, 514, 630
NlaIV GGNNCC 2 cut(s) 146, 533
NmuCI GTSAC 1 cut(s) 335
OliI CACNNNNGTG 1 cut(s) 333
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PctI GAATGC 1 cut(s) 477
PfeI GAWTC 2 cut(s) 654, 764
Pfl23II CGTACG 1 cut(s) 759
PkrI GCNGC 7 cut(s) 105, 232, 235, 278, 290, 442, 757
PspFI CCCAGC 3 cut(s) 262, 364, 641
PspLI CGTACG 1 cut(s) 759
PspN4I GGNNCC 2 cut(s) 146, 533
PspPI GGNCC 1 cut(s) 145
PstI CTGCAG 1 cut(s) 278
PsuI RGATCY 2 cut(s) 416, 579
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 4 cut(s) 195, 533, 745, 761
RsaNI GTAC 4 cut(s) 194, 532, 744, 760
RseI CAYNNNNRTG 1 cut(s) 333
SatI GCNGC 7 cut(s) 104, 231, 234, 277, 289, 441, 756
Sau3AI GATC 5 cut(s) 177, 416, 513, 579, 724
Sau96I GGNCC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 379
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 1 cut(s) 617
SfcI CTRYAG 1 cut(s) 274
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 699
SmoI CTYRAG 1 cut(s) 699
Sse9I AATT 2 cut(s) 476, 619
SspI AATATT 1 cut(s) 217
SspMI CTAG 4 cut(s) 189, 318, 525, 543
StyD4I CCNGG 1 cut(s) 377
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 4 cut(s) 117, 245, 425, 772
TaiI ACGT 1 cut(s) 455
TaqI TCGA 7 cut(s) 180, 206, 257, 649, 690, 727, 775
TaqII GACCGA 1 cut(s) 396
TasI AATT 2 cut(s) 476, 619
TatI WGTACW 1 cut(s) 193
TfiI GAWTC 2 cut(s) 654, 764
TscAI CASTG 1 cut(s) 575
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 7 cut(s) 103, 230, 233, 276, 288, 440, 755
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 296
TspRI CASTG 1 cut(s) 575
VpaK11BI GGWCC 1 cut(s) 145
XbaI TCTAGA 1 cut(s) 188
XmaJI CCTAGG 1 cut(s) 542
XmiI GTMKAC 1 cut(s) 548
XspI CTAG 4 cut(s) 189, 318, 525, 543
ZraI GACGTC 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.