FvH4_2g39590

Gamma carbonic anhydrase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012857
Physical Location & Seq
Reverse (-)
28401541 .. 28405394
3854 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39590.t1

Sequence Viewer

Length: 819 bp
ATGGGGACTTTGGGAAGAGCGATATACACCGTCGGATTCTGGATTAGGGAGACGGGCCAGGCCGTCGATCGCCTCGGCAGCCGCCTCCAGGGGAGCTACTACTTCAAGGAGCAGCTGTCTAGGCATCGGACTCTTATGAATATATTTGATAAAGCTCCTGTGGTTGACAAGGATGCATTCGTGGCTCCAAGTGCCTCTGTCATTGGTGATGTTCAGGTGGGAAAAGGATCTTCTATTTGGTATGGATGTGTATTGAGAGGTGACGTGAACAACATTGTCATTGGAGCTGGAACTAACATACAGGACAACTCTCTTGTGCATGTGGCAAAGTCTAATTTAAGTGGGAAGGTGTTGCCAACCATTATTGGTGATAATGTTACAGTAGGTCACAGTGCTGTTGTACATGGCTGTACGGTTGAGGATGAGGCCTTTGTTGGTATGGGAGCCACACTGCTTGATGGTGTCGTCGTTGAGAAACATGCTATGGTTGCTGCTGGAGCCCTTGTGAGACAGAATACAAGAATCCCCAGTGGAGAGGTTTGGGCAGGGAATCCCGCTAAATTTCTGAGGAAACTCACAGATGAAGAAATAGCATTCATCTCCCAGTCAGCCACCAATTATGTCAACCTTGCACAAGTCCATGCAGCGGAGAATGGGAAGTCCTTTGATGAGATTGAGTTTGAGAAGGTCCTCCGTAAGAAGTTTGCTCGTCGTGATGAGGAGTATGACTCAATGCTGGGTGTTGTTCGTGAAATTCCCCCAGAGCTTATTCTTCCCGATAATGTATTACCAGATAAAGCACTCAAGGCAGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hexapep

Protein Analysis

273

Amino Acids

29.54

Weight (kDa)

6.1

Isoelectric Point (pI)

31.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LbH_EIF2B PF25084 90 - 176 1.1e-06 EIF2B subunit epsilon LbH domain
Hexapep PF00132 119 - 151 1e-05 Bacterial transferase hexapeptide (six repeats)
Hexapep_2 PF14602 119 - 151 3.9e-06 Hexapeptide repeat of succinyl-transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47260
fragaria_vesca FvH4_2g39590
malus_domestica MD08G1029100.v1.1 MD15G1024600.v1.1
prunus_persica Prupe.1G376900_v2.0.a1 Prupe.1G376900_v2.0.a1
pyrus_communis pycom08g02290 pycom15g02120
rosa_chinensis RchiOBHm_Chr6g0301331
rosa_multiflora Rmu_sc0003197.1_g000011 Rmu_sc0013354.1_g000021
rosa_roxburghii Rroxscaffold_7G00166910
rosa_rugosa Rorug06G0303700 Rorug07G0031300
rosa_samantha Rh6BG423900 Rh6CG431100 Rh6DG417000
rosa_wichuraiana Rw6G036110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 82, 555, 647
AclWI GGATC 1 cut(s) 235
AcsI RAATTY 2 cut(s) 560, 753
AfaI GTAC 2 cut(s) 402, 412
AfiI CCNNNNNNNGG 2 cut(s) 88, 646
AgsI TTSAA 1 cut(s) 106
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 2 cut(s) 57, 87
AluBI AGCT 5 cut(s) 96, 115, 155, 287, 766
AluI AGCT 5 cut(s) 96, 115, 155, 287, 766
Alw26I GTCTC 2 cut(s) 44, 502
AlwI GGATC 1 cut(s) 235
AoxI GGCC 3 cut(s) 55, 60, 426
ApeKI GCWGC 4 cut(s) 78, 112, 491, 644
ApoI RAATTY 2 cut(s) 560, 753
AspS9I GGNCC 2 cut(s) 55, 688
AsuHPI GGTGA 3 cut(s) 218, 272, 380
AvaII GGWCC 1 cut(s) 688
BanII GRGCYC 1 cut(s) 502
BbvI GCAGC 4 cut(s) 90, 124, 478, 656
BccI CCATC 1 cut(s) 452
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 2 cut(s) 59, 89
BcoDI GTCTC 2 cut(s) 44, 502
BfaI CTAG 1 cut(s) 120
BisI GCNGC 5 cut(s) 79, 82, 113, 492, 645
BlsI GCNGC 5 cut(s) 80, 83, 114, 493, 646
Bme1390I CCNGG 2 cut(s) 59, 89
Bme18I GGWCC 1 cut(s) 688
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 2 cut(s) 55, 688
BmiI GGNNCC 3 cut(s) 186, 445, 499
BmrFI CCNGG 2 cut(s) 59, 89
BmrI ACTGGG 2 cut(s) 522, 598
BmsI GCATC 2 cut(s) 133, 163
BmuI ACTGGG 2 cut(s) 522, 598
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpmI CTGGAG 2 cut(s) 71, 516
BpuEI CTTGAG 1 cut(s) 788
BsaJI CCNNGG 2 cut(s) 73, 88
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 489, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 646
Bse1I ACTGG 2 cut(s) 528, 604
BseBI CCWGG 2 cut(s) 59, 89
BseDI CCNNGG 2 cut(s) 73, 88
BseGI GGATG 3 cut(s) 178, 251, 427
BseLI CCNNNNNNNGG 2 cut(s) 88, 646
BseMII CTCAG 1 cut(s) 557
BseNI ACTGG 2 cut(s) 528, 604
BseRI GAGGAG 1 cut(s) 734
BseXI GCAGC 4 cut(s) 90, 124, 478, 656
BseYI CCCAGC 1 cut(s) 736
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 3 cut(s) 57, 62, 428
BsiEI CGRYCG 1 cut(s) 70
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 2 cut(s) 88, 646
BsmAI GTCTC 2 cut(s) 44, 502
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 2 cut(s) 176, 593
BsnI GGCC 3 cut(s) 57, 62, 428
Bsp1286I GDGCHC 1 cut(s) 502
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 2 cut(s) 67, 227
BspACI CCGC 3 cut(s) 82, 555, 647
BspANI GGCC 3 cut(s) 57, 62, 428
BspCNI CTCAG 1 cut(s) 558
BspLI GGNNCC 3 cut(s) 186, 445, 499
BspPI GGATC 1 cut(s) 235
BspQI GCTCTTC 1 cut(s) 10
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 2 cut(s) 528, 604
BssECI CCNNGG 2 cut(s) 73, 88
BssMI GATC 2 cut(s) 67, 227
Bst2UI CCWGG 2 cut(s) 59, 89
Bst4CI ACNGT 4 cut(s) 31, 382, 392, 415
Bst6I CTCTTC 1 cut(s) 10
BstAUI TGTACA 1 cut(s) 400
BstDEI CTNAG 1 cut(s) 566
BstF5I GGATG 3 cut(s) 178, 251, 427
BstKTI GATC 2 cut(s) 70, 230
BstMAI GTCTC 2 cut(s) 44, 502
BstMBI GATC 2 cut(s) 67, 227
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 7 cut(s) 78, 121, 182, 191, 488, 497, 806
BstNI CCWGG 2 cut(s) 59, 89
BstNSI RCATGY 2 cut(s) 323, 482
BstSCI CCNGG 2 cut(s) 57, 87
BstV1I GCAGC 4 cut(s) 90, 124, 478, 656
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BsuRI GGCC 3 cut(s) 57, 62, 428
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 3 cut(s) 178, 251, 427
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 3 cut(s) 397, 449, 535
Cfr13I GGNCC 2 cut(s) 55, 688
Csp6I GTAC 2 cut(s) 401, 411
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 4 cut(s) 320, 404, 479, 641
CviQI GTAC 2 cut(s) 401, 411
DdeI CTNAG 1 cut(s) 566
DpnI GATC 2 cut(s) 69, 229
DpnII GATC 2 cut(s) 67, 227
Eam1104I CTCTTC 1 cut(s) 10
EarI CTCTTC 1 cut(s) 10
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 688
EcoO109I RGGNCCY 1 cut(s) 688
EcoRII CCWGG 2 cut(s) 57, 87
EcoT22I ATGCAT 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 502
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 4 cut(s) 323, 407, 482, 644
FaqI GGGAC 1 cut(s) 19
FatI CATG 4 cut(s) 319, 403, 478, 640
FauI CCCGC 1 cut(s) 562
Fnu4HI GCNGC 5 cut(s) 79, 82, 113, 492, 645
FokI GGATG 3 cut(s) 185, 258, 434
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 5 cut(s) 79, 82, 113, 492, 645
FspBI CTAG 1 cut(s) 120
GluI GCNGC 5 cut(s) 79, 82, 113, 492, 645
GsaI CCCAGC 1 cut(s) 740
GsuI CTGGAG 2 cut(s) 71, 516
HaeIII GGCC 3 cut(s) 57, 62, 428
Hin1II CATG 4 cut(s) 323, 407, 482, 644
HincII GTYRAC 2 cut(s) 166, 625
HindII GTYRAC 2 cut(s) 166, 625
HinfI GANTC 5 cut(s) 36, 130, 522, 550, 728
HphI GGTGA 3 cut(s) 218, 272, 380
Hpy166II GTNNAC 3 cut(s) 166, 268, 625
Hpy188I TCNGA 3 cut(s) 35, 129, 567
Hpy188III TCNNGA 4 cut(s) 40, 713, 749, 776
Hpy8I GTNNAC 3 cut(s) 166, 268, 625
Hpy99I CGWCG 4 cut(s) 35, 68, 470, 714
HpyAV CCTTC 2 cut(s) 340, 679
HpyCH4III ACNGT 4 cut(s) 31, 382, 392, 415
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 4 cut(s) 176, 319, 632, 644
HpyF10VI GCNNNNNNNGC 7 cut(s) 78, 121, 182, 191, 488, 497, 806
HpyF3I CTNAG 1 cut(s) 566
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 4 cut(s) 323, 407, 482, 644
Kzo9I GATC 2 cut(s) 67, 227
LguI GCTCTTC 1 cut(s) 10
LmnI GCTCC 7 cut(s) 93, 109, 160, 190, 284, 443, 497
Lsp1109I GCAGC 4 cut(s) 90, 124, 478, 656
LweI GCATC 2 cut(s) 133, 163
MaeI CTAG 1 cut(s) 120
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 3 cut(s) 260, 376, 386
MalI GATC 2 cut(s) 69, 229
MboI GATC 2 cut(s) 67, 227
MboII GAAGA 4 cut(s) 27, 222, 596, 764
MflI RGATCY 1 cut(s) 227
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 4 cut(s) 334, 560, 616, 753
MlyI GAGTC 2 cut(s) 124, 722
MmeI TCCRAC 1 cut(s) 13
Mph1103I ATGCAT 1 cut(s) 178
MseI TTAA 1 cut(s) 338
MspA1I CMGCKG 2 cut(s) 115, 647
MspR9I CCNGG 2 cut(s) 59, 89
Mva1269I GAATGC 2 cut(s) 176, 593
MvaI CCWGG 2 cut(s) 59, 89
MwoI GCNNNNNNNGC 7 cut(s) 78, 121, 182, 191, 488, 497, 806
NdeII GATC 2 cut(s) 67, 227
NlaIII CATG 4 cut(s) 323, 407, 482, 644
NlaIV GGNNCC 3 cut(s) 186, 445, 499
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 2 cut(s) 260, 386
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 2 cut(s) 323, 482
PceI AGGCCT 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 10
PctI GAATGC 2 cut(s) 176, 593
PfeI GAWTC 3 cut(s) 36, 522, 550
PkrI GCNGC 5 cut(s) 80, 83, 114, 493, 646
Ple19I CGATCG 1 cut(s) 70
PleI GAGTC 2 cut(s) 124, 722
PpsI GAGTC 2 cut(s) 124, 722
PpuMI RGGWCCY 1 cut(s) 688
Psp5II RGGWCCY 1 cut(s) 688
Psp6I CCWGG 2 cut(s) 57, 87
PspFI CCCAGC 1 cut(s) 736
PspGI CCWGG 2 cut(s) 57, 87
PspN4I GGNNCC 3 cut(s) 186, 445, 499
PspPI GGNCC 2 cut(s) 55, 688
PspPPI RGGWCCY 1 cut(s) 688
PsuI RGATCY 1 cut(s) 227
PvuI CGATCG 1 cut(s) 70
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 2 cut(s) 402, 412
RsaNI GTAC 2 cut(s) 401, 411
SapI GCTCTTC 1 cut(s) 10
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 5 cut(s) 79, 82, 113, 492, 645
Sau3AI GATC 2 cut(s) 67, 227
Sau96I GGNCC 2 cut(s) 55, 688
SchI GAGTC 2 cut(s) 124, 722
ScrFI CCNGG 2 cut(s) 59, 89
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 2 cut(s) 133, 163
SinI GGWCC 1 cut(s) 688
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
Sse9I AATT 4 cut(s) 334, 560, 616, 753
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 3 cut(s) 82, 555, 647
SspMI CTAG 1 cut(s) 120
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 2 cut(s) 57, 87
TaaI ACNGT 4 cut(s) 31, 382, 392, 415
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 66
TasI AATT 4 cut(s) 334, 560, 616, 753
TatI WGTACW 1 cut(s) 400
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 3 cut(s) 36, 522, 550
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 3 cut(s) 397, 456, 535
TseFI GTSAC 2 cut(s) 260, 386
TseI GCWGC 4 cut(s) 78, 112, 491, 644
Tsp45I GTSAC 2 cut(s) 260, 386
TspDTI ATGAA 3 cut(s) 152, 586, 597
TspGWI ACGGA 1 cut(s) 683
TspRI CASTG 3 cut(s) 397, 456, 535
VpaK11BI GGWCC 1 cut(s) 688
XapI RAATTY 2 cut(s) 560, 753
XceI RCATGY 2 cut(s) 323, 482
XspI CTAG 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.