MD15G1024600.v1.1

Gamma carbonic anhydrase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012857
Physical Location & Seq
Reverse (-)
1460241 .. 1464339
4099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1024600.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGGGACTTTAGGACGAGCAATATACACTGTCGGATTCTGGATTCGGGAGACGGGCCAGGCCATTGATCGCCTCGGCAGCAGCCTCCAGGGCAGCTACTACTTCAAAGAGCAGCTGTCTCAGCATAGAACTCTGATGAACGTATTCAATAAAGCTCCTGTGGTTGACAAGGATGTGTTTGTGGCGCCAAGTGCCTCCATCATTGGCGATGTTCAAGTGGGAAGAGGGTCTTCCATATGGTATGGATGTGTTTTAAGAGGTGATGTGAACAACATCGTTGTTGGATATGGAACTAACATACAGGACAACTCCCTTGTTCATGTGGCGAAGTCAAATTTAAGTGGGAAGGTGTTACCAACCATTATTGGAGATAATGTTACTGTAGGTCACAGTGCTGTTATACATGGCTGTACTGTTGAGGATGAGGCCTTTGTTGGCATGGGAGCCACACTTTTGGATGGCGTCGTCGTTGAGAAACATGCCATGGTAGCTGCTGGATCCCTGGTGAGACAGAATACAAAAATCCCCAGTGGAGAGGTATGGGCAGGAAACCCAGCAAAATTTCTGAGAAAGCTCACAGATGAAGAAATAGCCTTCATCACCCAGTCAGCCACCAATTACACCAACCTTGCACAAGTCCATGCAGCTGAGAATGTGAAGTCGTTTGATGAGATTGAGTTTGAGAAGGCCCTCCGCAAGAAGTTTGCCCACAAGGATGAGGAGTATGACTCAATGCTAGGTGTCGTTCGTGAGATTCCCCCAGAACTTATTCTTCCCGATAATGTATTACCAGATAAAGCAGCAAAGGACCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hexapep

Protein Analysis

273

Amino Acids

29.57

Weight (kDa)

5.82

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hexapep PF00132 54 - 87 7.8e-06 Bacterial transferase hexapeptide (six repeats)
LbH_EIF2B PF25084 90 - 176 2.5e-06 EIF2B subunit epsilon LbH domain
Hexapep PF00132 119 - 151 6.9e-06 Bacterial transferase hexapeptide (six repeats)
Hexapep_2 PF14602 119 - 151 2.6e-06 Hexapeptide repeat of succinyl-transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47260
fragaria_vesca FvH4_2g39590
malus_domestica MD08G1029100.v1.1 MD15G1024600.v1.1
prunus_persica Prupe.1G376900_v2.0.a1 Prupe.1G376900_v2.0.a1
pyrus_communis pycom08g02290 pycom15g02120
rosa_chinensis RchiOBHm_Chr6g0301331
rosa_multiflora Rmu_sc0003197.1_g000011 Rmu_sc0013354.1_g000021
rosa_roxburghii Rroxscaffold_7G00166910
rosa_rugosa Rorug06G0303700 Rorug07G0031300
rosa_samantha Rh6BG423900 Rh6CG431100 Rh6DG417000
rosa_wichuraiana Rw6G036110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AciI CCGC 1 cut(s) 694
AclWI GGATC 2 cut(s) 492, 505
AcsI RAATTY 2 cut(s) 334, 560
AcyI GRCGYC 2 cut(s) 185, 462
AfaI GTAC 1 cut(s) 412
AgsI TTSAA 3 cut(s) 106, 148, 215
AjnI CCWGG 3 cut(s) 57, 87, 501
AluBI AGCT 6 cut(s) 96, 115, 155, 491, 574, 647
AluI AGCT 6 cut(s) 96, 115, 155, 491, 574, 647
Alw26I GTCTC 3 cut(s) 44, 123, 502
AlwI GGATC 2 cut(s) 492, 505
AoxI GGCC 4 cut(s) 55, 60, 426, 687
ApeKI GCWGC 7 cut(s) 78, 81, 93, 112, 491, 644, 800
ApoI RAATTY 2 cut(s) 334, 560
Asp700I GAANNNNTTC 2 cut(s) 143, 768
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 3 cut(s) 55, 688, 808
AsuHPI GGTGA 3 cut(s) 272, 517, 592
AvaII GGWCC 1 cut(s) 808
BamHI GGATCC 1 cut(s) 497
BanI GGYRCC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 7 cut(s) 90, 93, 105, 124, 478, 656, 812
BccI CCATC 2 cut(s) 206, 452
BciT130I CCWGG 3 cut(s) 59, 89, 503
BcoDI GTCTC 3 cut(s) 44, 123, 502
BfaI CTAG 1 cut(s) 737
BfmI CTRYAG 1 cut(s) 381
BfoI RGCGCY 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 90
BisI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
BlsI GCNGC 7 cut(s) 80, 83, 95, 114, 493, 646, 802
Bme1390I CCNGG 3 cut(s) 59, 89, 503
Bme18I GGWCC 1 cut(s) 808
BmgT120I GGNCC 3 cut(s) 55, 688, 808
BmiI GGNNCC 3 cut(s) 186, 445, 499
BmrFI CCNGG 3 cut(s) 59, 89, 503
BmrI ACTGGG 2 cut(s) 522, 598
BmuI ACTGGG 2 cut(s) 522, 598
BpiI GAAGAC 1 cut(s) 222
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpmI CTGGAG 1 cut(s) 71
BsaHI GRCGYC 2 cut(s) 185, 462
BsaJI CCNNGG 4 cut(s) 73, 88, 483, 501
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bse1I ACTGG 2 cut(s) 528, 604
BseBI CCWGG 3 cut(s) 59, 89, 503
BseDI CCNNGG 4 cut(s) 73, 88, 483, 501
BseGI GGATG 5 cut(s) 178, 251, 427, 463, 721
BseMII CTCAG 3 cut(s) 134, 557, 639
BseNI ACTGG 2 cut(s) 528, 604
BseRI GAGGAG 1 cut(s) 734
BseXI GCAGC 7 cut(s) 90, 93, 105, 124, 478, 656, 812
BseYI CCCAGC 1 cut(s) 553
BshFI GGCC 4 cut(s) 57, 62, 428, 689
BshNI GGYRCC 1 cut(s) 184
BslFI GGGAC 1 cut(s) 19
BsmAI GTCTC 3 cut(s) 44, 123, 502
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 4 cut(s) 57, 62, 428, 689
Bsp143I GATC 2 cut(s) 67, 497
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 1 cut(s) 694
BspANI GGCC 4 cut(s) 57, 62, 428, 689
BspCNI CTCAG 3 cut(s) 133, 558, 640
BspLI GGNNCC 3 cut(s) 186, 445, 499
BspPI GGATC 2 cut(s) 492, 505
BspT107I GGYRCC 1 cut(s) 184
BsrI ACTGG 2 cut(s) 528, 604
BssECI CCNNGG 4 cut(s) 73, 88, 483, 501
BssMI GATC 2 cut(s) 67, 497
BssNI GRCGYC 2 cut(s) 185, 462
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 3 cut(s) 59, 89, 503
Bst4CI ACNGT 4 cut(s) 31, 382, 392, 415
Bst6I CTCTTC 1 cut(s) 217
BstACI GRCGYC 2 cut(s) 185, 462
BstDEI CTNAG 3 cut(s) 120, 566, 648
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 5 cut(s) 178, 251, 427, 463, 721
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 2 cut(s) 70, 500
BstMAI GTCTC 3 cut(s) 44, 123, 502
BstMBI GATC 2 cut(s) 67, 497
BstMWI GCNNNNNNNGC 5 cut(s) 78, 90, 121, 191, 488
BstNI CCWGG 3 cut(s) 59, 89, 503
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 3 cut(s) 57, 87, 501
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 7 cut(s) 90, 93, 105, 124, 478, 656, 812
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 497
BstXI CCANNNNNNTGG 1 cut(s) 454
BstYI RGATCY 1 cut(s) 497
BsuRI GGCC 4 cut(s) 57, 62, 428, 689
BtgI CCRYGG 1 cut(s) 483
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 5 cut(s) 178, 251, 427, 463, 721
BtsIMutI CAGTG 3 cut(s) 27, 397, 535
CfoI GCGC 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 55, 688, 808
CseI GACGC 1 cut(s) 451
Csp6I GTAC 1 cut(s) 411
CviAII CATG 6 cut(s) 320, 404, 439, 479, 484, 641
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 3 cut(s) 120, 566, 648
DinI GGCGCC 1 cut(s) 186
DpnI GATC 2 cut(s) 69, 499
DpnII GATC 2 cut(s) 67, 497
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 483
Eco147I AGGCCT 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 808
EcoO109I RGGNCCY 1 cut(s) 688
EcoRII CCWGG 3 cut(s) 57, 87, 501
EcoT14I CCWWGG 1 cut(s) 483
EgeI GGCGCC 1 cut(s) 186
EheI GGCGCC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 6 cut(s) 323, 407, 442, 482, 487, 644
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 19
FatI CATG 6 cut(s) 319, 403, 438, 478, 483, 640
FauNDI CATATG 1 cut(s) 236
Fnu4HI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
FokI GGATG 5 cut(s) 185, 258, 434, 470, 728
Fsp4HI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
FspBI CTAG 1 cut(s) 737
GlaI GCGC 1 cut(s) 186
GluI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
GsaI CCCAGC 1 cut(s) 557
GsuI CTGGAG 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 4 cut(s) 57, 62, 428, 689
HgaI GACGC 1 cut(s) 451
HhaI GCGC 1 cut(s) 187
Hin1I GRCGYC 2 cut(s) 185, 462
Hin1II CATG 6 cut(s) 323, 407, 442, 482, 487, 644
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 4 cut(s) 36, 43, 728, 754
HphI GGTGA 3 cut(s) 272, 517, 592
Hpy166II GTNNAC 2 cut(s) 166, 268
Hpy188I TCNGA 3 cut(s) 35, 135, 567
Hpy188III TCNNGA 4 cut(s) 40, 47, 749, 776
Hpy8I GTNNAC 2 cut(s) 166, 268
Hpy99I CGWCG 2 cut(s) 467, 470
HpyAV CCTTC 3 cut(s) 340, 604, 679
HpyCH4III ACNGT 4 cut(s) 31, 382, 392, 415
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 2 cut(s) 632, 644
HpyF10VI GCNNNNNNNGC 5 cut(s) 78, 90, 121, 191, 488
HpyF3I CTNAG 3 cut(s) 120, 566, 648
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 2 cut(s) 185, 462
Hsp92II CATG 6 cut(s) 323, 407, 442, 482, 487, 644
HspAI GCGC 1 cut(s) 185
KasI GGCGCC 1 cut(s) 184
Kzo9I GATC 2 cut(s) 67, 497
LmnI GCTCC 2 cut(s) 160, 443
Lsp1109I GCAGC 7 cut(s) 90, 93, 105, 124, 478, 656, 812
MaeI CTAG 1 cut(s) 737
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 351, 376, 386
MalI GATC 2 cut(s) 69, 499
MboI GATC 2 cut(s) 67, 497
MboII GAAGA 4 cut(s) 222, 234, 596, 764
MflI RGATCY 1 cut(s) 497
MluCI AATT 3 cut(s) 334, 560, 616
Mly113I GGCGCC 1 cut(s) 185
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 2 cut(s) 13, 262
MroXI GAANNNNTTC 2 cut(s) 143, 768
MseI TTAA 2 cut(s) 254, 338
MslI CAYNNNNRTG 1 cut(s) 714
MspA1I CMGCKG 2 cut(s) 115, 647
MspR9I CCNGG 3 cut(s) 59, 89, 503
MvaI CCWGG 3 cut(s) 59, 89, 503
MwoI GCNNNNNNNGC 5 cut(s) 78, 90, 121, 191, 488
NarI GGCGCC 1 cut(s) 185
NcoI CCATGG 1 cut(s) 483
NdeI CATATG 1 cut(s) 236
NdeII GATC 2 cut(s) 67, 497
NlaIII CATG 6 cut(s) 323, 407, 442, 482, 487, 644
NlaIV GGNNCC 3 cut(s) 186, 445, 499
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 1 cut(s) 386
NspI RCATGY 1 cut(s) 482
PceI AGGCCT 1 cut(s) 428
PdmI GAANNNNTTC 2 cut(s) 143, 768
PfeI GAWTC 3 cut(s) 36, 43, 754
PkrI GCNGC 7 cut(s) 80, 83, 95, 114, 493, 646, 802
PleI GAGTC 1 cut(s) 722
PluTI GGCGCC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 722
Psp6I CCWGG 3 cut(s) 57, 87, 501
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 3 cut(s) 57, 87, 501
PspN4I GGNNCC 3 cut(s) 186, 445, 499
PspPI GGNCC 3 cut(s) 55, 688, 808
PsuI RGATCY 1 cut(s) 497
PvuII CAGCTG 2 cut(s) 115, 647
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 1 cut(s) 714
SaqAI TTAA 2 cut(s) 254, 338
SatI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
Sau3AI GATC 2 cut(s) 67, 497
Sau96I GGNCC 3 cut(s) 55, 688, 808
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 3 cut(s) 59, 89, 503
SfcI CTRYAG 1 cut(s) 381
SfoI GGCGCC 1 cut(s) 186
SinI GGWCC 1 cut(s) 808
SmiMI CAYNNNNRTG 1 cut(s) 714
Sse9I AATT 3 cut(s) 334, 560, 616
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 1 cut(s) 694
SspDI GGCGCC 1 cut(s) 184
SspMI CTAG 1 cut(s) 737
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 57, 87, 501
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 4 cut(s) 31, 382, 392, 415
TaiI ACGT 1 cut(s) 144
TasI AATT 3 cut(s) 334, 560, 616
TatI WGTACW 1 cut(s) 410
TfiI GAWTC 3 cut(s) 36, 43, 754
Tru1I TTAA 2 cut(s) 254, 338
Tru9I TTAA 2 cut(s) 254, 338
TscAI CASTG 3 cut(s) 34, 397, 535
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 7 cut(s) 78, 81, 93, 112, 491, 644, 800
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 4 cut(s) 152, 308, 586, 597
TspRI CASTG 3 cut(s) 34, 397, 535
VpaK11BI GGWCC 1 cut(s) 808
XapI RAATTY 2 cut(s) 334, 560
XceI RCATGY 1 cut(s) 482
XmnI GAANNNNTTC 2 cut(s) 143, 768
XspI CTAG 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.