Rorug06G0303700

Gamma carbonic anhydrase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012857
Physical Location & Seq
Forward (+)
45487343 .. 45516143
28801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0303700.1

Sequence Viewer

Length: 1698 bp
ATGAGATTCCTTACACATCATTCGTACTTCTCTCTCCCTTACATCCTCTACTCTCCTATAAATACCAACAGTCTACTCGCCGCTTCACCACAACCCACAACTAGCTATCTGCCACTGATCATCACTATGGCCAAGCATTTAGCTCTCGCTTTCTCTCTTTGCTCTCTTATTCTCTTCCACGGCTGCTTAGCCAGTCGCCAACAATCTCAGTTCAGCCAGCAAAATGAGTGCCAGCTGAACCAGATCCAAGCCCGCGAGCCCGACAACCGCATTGAGTGCGAAGCAGGTCGGATCGAGTCCTGGGACTACAACCAGGATGACTTCCAGTGCGCTGGTGTCGCCGTTCAGAGAATCACCATTGAGAGCAACGGCCTTCACTTGCCATCTTACACTAACACTCCACGACTAGTTTACGTCGTCCAAGGTAGGGGTCATGTAGGAATGGTGCTCCCTGGCTGTCCCGAGACTTTTGAAGAATCCGAGCAATCTGATGAGGAACAACAGGGCCAGCAACAACAAGGGCAAGGCCAACGAGGCCGCCAGCAGCAGGGACGCCGACAGGGACAATTCGGCCAGCAAGGCCAACAGGGACAACAAGAGAGCTTTGAGCAGCTAGACCGTCACCAGAGGGTCCGAATCGTCAAGGAAGGTGATGTCATTGCCGTCCCCGCCGGAGTCACCTGCTGGACTCACAACCACGGTAACCAACCTCTTGTTTTCGTCAGTCTTTTTGATATGAGCAACGAGCTCAACCAGCTTGACCAGAACCCAAGGAAATTTTATCTTGCTGGTAATCCACAAGATGAGTTCAACCAACAAGGCCAAAGCCAATGGGGACAACAAGGACAACAAGGCCGTGGACAACAAGGAAGGACCAGGCGCCAACAGCAGGGAAACAACAACAACAACATCTTTGCCGGCTTCGACACACAGCTCCTCGCCGACGCTCTCAACATCGACCAACAGACCGCCCAGCGGCTCCAGGGACAGAACGACAACAGGCCACAGATTGTTAGGGTTCAGGGACGTTTCGACTTTGTCGAGCCACCACGGTCACAGAGGGAGGAAAGACAAATGCAAGGACAACAAATGCAGGGACAACAAGGACAGTATGAACAGAATGGCATGGAGGAGCTCTTCTGCCACATGCAGATGAAACAGAACATCGGCAAACCTAAAATGGCCGACGTCTACAGCCCGCAAGCCGGTCGCATCAGCACCCTCAATAGCTTCAGCATGCCTATTCTCAGGCGCCTCCGCCTGAGTGCCGAGAGAGGCTTCTTCTACAACAACGCTATCTACAGCCCACACTGGAACTTGAACGCCCATGAAGTCTACTACGTCATCAGGGGCAGCGCTAGGGTTCAGGTGGTGAACGACAATGGCGAGACCATCTTGGACGACCAGGTCCGCCAGGGACAGGTCTTCATCGTGCCACAGAACCACGCCGTGTTGCAAAAGGCCATGAGCAACGGATACGAGTACATTGCCTTCAAGACCGATGACAATGCCATGATGAACACCATGGCTGGCAGGACCTCCGTCCTGCGGGCACTCCCTGACGTGGTCCTCGCCAACGCCTACAAGATGGACCGTGAGCAGGCAAGGAACCTCAAGTACAACAGGCAGGAGACCGTTGCTTTGAGTTCCTCAAGGTCTTCTCGGTCCCAGAGGAGCTGGTGGGCTCTTGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hexapep

Protein Analysis

565

Amino Acids

64.47

Weight (kDa)

7.21

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 83 - 266 2.5e-25 Cupin
Cupin_1 PF00190 388 - 535 1.8e-38 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47260
fragaria_vesca FvH4_2g39590
malus_domestica MD08G1029100.v1.1 MD15G1024600.v1.1
prunus_persica Prupe.1G376900_v2.0.a1 Prupe.1G376900_v2.0.a1
pyrus_communis pycom08g02290 pycom15g02120
rosa_chinensis RchiOBHm_Chr6g0301331
rosa_multiflora Rmu_sc0003197.1_g000011 Rmu_sc0013354.1_g000021
rosa_roxburghii Rroxscaffold_7G00166910
rosa_rugosa Rorug06G0303700 Rorug07G0031300
rosa_samantha Rh6BG423900 Rh6CG431100 Rh6DG417000
rosa_wichuraiana Rw6G036110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 689
AasI GACNNNNNNGTC 1 cut(s) 1406
AatII GACGTC 1 cut(s) 1191
Acc36I ACCTGC 2 cut(s) 275, 689
AccB1I GGYRCC 2 cut(s) 879, 1251
AccI GTMKAC 3 cut(s) 73, 1191, 1335
AccII CGCG 1 cut(s) 255
AclWI GGATC 2 cut(s) 238, 299
AcoI YGGCCR 3 cut(s) 129, 571, 1182
AcsI RAATTY 1 cut(s) 776
AcuI CTGAAG 1 cut(s) 1216
AcyI GRCGYC 4 cut(s) 553, 880, 1188, 1252
AdeI CACNNNGTG 1 cut(s) 1450
AfaI GTAC 3 cut(s) 26, 1484, 1619
AfeI AGCGCT 1 cut(s) 1357
AfiI CCNNNNNNNGG 9 cut(s) 427, 547, 889, 975, 1205, 1420, 1548, 1564, 1600
AgsI TTSAA 4 cut(s) 473, 811, 1321, 1495
AhlI ACTAGT 1 cut(s) 406
AjiI CACGTC 1 cut(s) 1564
AjnI CCWGG 7 cut(s) 299, 312, 451, 875, 981, 1404, 1413
Alw21I GWGCWC 3 cut(s) 450, 750, 1137
Alw26I GTCTC 3 cut(s) 458, 1382, 1625
AlwI GGATC 2 cut(s) 238, 299
Ama87I CYCGRG 1 cut(s) 461
Aor51HI AGCGCT 1 cut(s) 1357
ApeKI GCWGC 4 cut(s) 183, 544, 610, 1353
ApoI RAATTY 1 cut(s) 776
ArsI GACNNNNNNTTYG 2 cut(s) 1624, 1656
AspLEI GCGC 4 cut(s) 332, 882, 1254, 1358
AspS9I GGNCC 8 cut(s) 505, 631, 873, 1408, 1536, 1567, 1591, 1665
AsuHPI GGTGA 6 cut(s) 78, 346, 614, 662, 670, 1384
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 7 cut(s) 631, 873, 1408, 1536, 1567, 1591, 1665
BaeGI GKGCMC 1 cut(s) 1555
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 2 cut(s) 879, 1251
BanII GRGCYC 4 cut(s) 261, 750, 1137, 1687
BarI GAAGNNNNNNTAC 2 cut(s) 1475, 1507
BbsI GAAGAC 2 cut(s) 1417, 1650
Bbv12I GWGCWC 3 cut(s) 450, 750, 1137
BbvI GCAGC 4 cut(s) 170, 556, 622, 1365
BccI CCATC 3 cut(s) 391, 1400, 1582
BceAI ACGGC 6 cut(s) 196, 326, 385, 647, 840, 1433
BcgI CGANNNNNNTGC 6 cut(s) 1190, 1224, 1469, 1490, 1503, 1524
BciT130I CCWGG 7 cut(s) 301, 314, 453, 877, 983, 1406, 1415
BciVI GTATCC 1 cut(s) 1469
BclI TGATCA 1 cut(s) 117
BcoDI GTCTC 3 cut(s) 458, 1382, 1625
BcuI ACTAGT 1 cut(s) 406
BfaI CTAG 4 cut(s) 102, 407, 614, 1359
BfmI CTRYAG 2 cut(s) 1192, 1300
BfoI RGCGCY 3 cut(s) 883, 1255, 1359
BfuAI ACCTGC 2 cut(s) 275, 689
BfuI GTATCC 1 cut(s) 1469
BglI GCCNNNNNGGC 2 cut(s) 534, 579
BisI GCNGC 7 cut(s) 81, 184, 538, 545, 611, 977, 1354
BlpI GCTNAGC 1 cut(s) 187
BlsI GCNGC 7 cut(s) 82, 185, 539, 546, 612, 978, 1355
Bme1390I CCNGG 7 cut(s) 301, 314, 453, 877, 983, 1406, 1415
Bme18I GGWCC 7 cut(s) 631, 873, 1408, 1536, 1567, 1591, 1665
BmeT110I CYCGRG 1 cut(s) 461
BmgBI CACGTC 1 cut(s) 1564
BmgT120I GGNCC 8 cut(s) 505, 631, 873, 1408, 1536, 1567, 1591, 1665
BmiI GGNNCC 6 cut(s) 632, 881, 980, 1253, 1610, 1667
BmrFI CCNGG 7 cut(s) 301, 314, 453, 877, 983, 1406, 1415
BmsI GCATC 2 cut(s) 1221, 1681
BoxI GACNNNNGTC 1 cut(s) 1541
BpiI GAAGAC 2 cut(s) 1417, 1650
BpmI CTGGAG 1 cut(s) 965
Bpu1102I GCTNAGC 1 cut(s) 187
BpuEI CTTGAG 2 cut(s) 1598, 1636
BsaHI GRCGYC 4 cut(s) 553, 880, 1188, 1252
BsaI GGTCTC 2 cut(s) 1382, 1625
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bsc4I CCNNNNNNNGG 9 cut(s) 427, 547, 889, 975, 1205, 1420, 1548, 1564, 1600
Bse118I RCCGGY 2 cut(s) 917, 1205
Bse1I ACTGG 3 cut(s) 192, 325, 1316
Bse3DI GCAATG 2 cut(s) 657, 1485
BseBI CCWGG 7 cut(s) 301, 314, 453, 877, 983, 1406, 1415
BseGI GGATG 2 cut(s) 42, 322
BseLI CCNNNNNNNGG 9 cut(s) 427, 547, 889, 975, 1205, 1420, 1548, 1564, 1600
BseMI GCAATG 2 cut(s) 657, 1485
BseMII CTCAG 3 cut(s) 221, 1253, 1261
BseNI ACTGG 3 cut(s) 192, 325, 1316
BseRI GAGGAG 3 cut(s) 926, 1145, 1687
BseSI GKGCMC 1 cut(s) 1555
BseXI GCAGC 4 cut(s) 170, 556, 622, 1365
BseYI CCCAGC 1 cut(s) 972
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 1210
BshNI GGYRCC 2 cut(s) 879, 1251
BsiEI CGRYCG 1 cut(s) 1210
BsiHKAI GWGCWC 3 cut(s) 450, 750, 1137
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 3 cut(s) 672, 918, 1206
BslI CCNNNNNNNGG 9 cut(s) 427, 547, 889, 975, 1205, 1420, 1548, 1564, 1600
BsmAI GTCTC 3 cut(s) 458, 1382, 1625
Bso31I GGTCTC 2 cut(s) 1382, 1625
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 6 cut(s) 261, 450, 750, 1137, 1555, 1687
Bsp143I GATC 3 cut(s) 117, 243, 291
Bsp1720I GCTNAGC 1 cut(s) 187
Bsp19I CCATGG 1 cut(s) 1524
BspCNI CTCAG 3 cut(s) 220, 1254, 1260
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 6 cut(s) 632, 881, 980, 1253, 1610, 1667
BspMI ACCTGC 2 cut(s) 275, 689
BspPI GGATC 2 cut(s) 238, 299
BspQI GCTCTTC 1 cut(s) 1142
BspT107I GGYRCC 2 cut(s) 879, 1251
BspTNI GGTCTC 2 cut(s) 1382, 1625
BsrDI GCAATG 2 cut(s) 657, 1485
BsrFI RCCGGY 2 cut(s) 917, 1205
BsrI ACTGG 3 cut(s) 192, 325, 1316
BssAI RCCGGY 2 cut(s) 917, 1205
BssMI GATC 3 cut(s) 117, 243, 291
BssNI GRCGYC 4 cut(s) 553, 880, 1188, 1252
BssT1I CCWWGG 3 cut(s) 421, 770, 1524
Bst2UI CCWGG 7 cut(s) 301, 314, 453, 877, 983, 1406, 1415
Bst4CI ACNGT 7 cut(s) 71, 620, 701, 1053, 1110, 1595, 1636
Bst6I CTCTTC 2 cut(s) 179, 1142
BstACI GRCGYC 4 cut(s) 553, 880, 1188, 1252
BstAPI GCANNNNNTGC 1 cut(s) 276
BstDEI CTNAG 4 cut(s) 187, 207, 1247, 1262
BstDSI CCRYGG 5 cut(s) 178, 697, 856, 1049, 1524
BstEII GGTNACC 1 cut(s) 701
BstF5I GGATG 2 cut(s) 42, 322
BstFNI CGCG 1 cut(s) 255
BstH2I RGCGCY 3 cut(s) 883, 1255, 1359
BstHHI GCGC 4 cut(s) 332, 882, 1254, 1358
BstKTI GATC 3 cut(s) 120, 246, 294
BstMAI GTCTC 3 cut(s) 458, 1382, 1625
BstMBI GATC 3 cut(s) 117, 243, 291
BstMCI CGRYCG 1 cut(s) 1210
BstMWI GCNNNNNNNGC 8 cut(s) 276, 338, 534, 579, 668, 754, 886, 1691
BstNI CCWGG 7 cut(s) 301, 314, 453, 877, 983, 1406, 1415
BstNSI RCATGY 2 cut(s) 1150, 1240
BstPAI GACNNNNGTC 1 cut(s) 1541
BstPI GGTNACC 1 cut(s) 701
BstSCI CCNGG 7 cut(s) 299, 312, 451, 875, 981, 1404, 1413
BstSFI CTRYAG 2 cut(s) 1192, 1300
BstSLI GKGCMC 1 cut(s) 1555
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 4 cut(s) 170, 556, 622, 1365
BstV2I GAAGAC 2 cut(s) 1417, 1650
BstX2I RGATCY 1 cut(s) 243
BstXI CCANNNNNNTGG 1 cut(s) 332
BstYI RGATCY 1 cut(s) 243
BsuI GTATCC 1 cut(s) 1469
BtgI CCRYGG 5 cut(s) 178, 697, 856, 1049, 1524
BtrI CACGTC 1 cut(s) 1564
BtsCI GGATG 2 cut(s) 42, 322
BtsIMutI CAGTG 3 cut(s) 113, 332, 1309
BveI ACCTGC 2 cut(s) 275, 689
CfoI GCGC 4 cut(s) 332, 882, 1254, 1358
Cfr10I RCCGGY 2 cut(s) 917, 1205
Cfr13I GGNCC 8 cut(s) 505, 631, 873, 1408, 1536, 1567, 1591, 1665
CseI GACGC 2 cut(s) 561, 953
CsiI ACCWGGT 1 cut(s) 1404
Csp6I GTAC 3 cut(s) 25, 1483, 1618
CviAII CATG 8 cut(s) 434, 1126, 1147, 1237, 1328, 1465, 1513, 1525
CviQI GTAC 3 cut(s) 25, 1483, 1618
DdeI CTNAG 4 cut(s) 187, 207, 1247, 1262
DinI GGCGCC 2 cut(s) 881, 1253
DpnI GATC 3 cut(s) 119, 245, 293
DpnII GATC 3 cut(s) 117, 243, 291
DraIII CACNNNGTG 1 cut(s) 1450
DrdI GACNNNNNNGTC 1 cut(s) 1406
DseDI GACNNNNNNGTC 1 cut(s) 1406
EaeI YGGCCR 3 cut(s) 129, 571, 1182
Eam1104I CTCTTC 2 cut(s) 179, 1142
EarI CTCTTC 2 cut(s) 179, 1142
EciI GGCGGA 2 cut(s) 1247, 1400
Ecl136II GAGCTC 2 cut(s) 748, 1135
Eco130I CCWWGG 3 cut(s) 421, 770, 1524
Eco24I GRGCYC 4 cut(s) 261, 750, 1137, 1687
Eco31I GGTCTC 2 cut(s) 1382, 1625
Eco47I GGWCC 7 cut(s) 631, 873, 1408, 1536, 1567, 1591, 1665
Eco47III AGCGCT 1 cut(s) 1357
Eco53kI GAGCTC 2 cut(s) 748, 1135
Eco57I CTGAAG 1 cut(s) 1216
Eco88I CYCGRG 1 cut(s) 461
Eco91I GGTNACC 1 cut(s) 701
EcoICRI GAGCTC 2 cut(s) 748, 1135
EcoO109I RGGNCCY 1 cut(s) 1536
EcoO65I GGTNACC 1 cut(s) 701
EcoRII CCWGG 7 cut(s) 299, 312, 451, 875, 981, 1404, 1413
EcoT14I CCWWGG 3 cut(s) 421, 770, 1524
EcoT38I GRGCYC 4 cut(s) 261, 750, 1137, 1687
EgeI GGCGCC 2 cut(s) 881, 1253
EheI GGCGCC 2 cut(s) 881, 1253
ErhI CCWWGG 3 cut(s) 421, 770, 1524
FaeI CATG 8 cut(s) 437, 1129, 1150, 1240, 1331, 1468, 1516, 1528
FatI CATG 8 cut(s) 433, 1125, 1146, 1236, 1327, 1464, 1512, 1524
FauI CCCGC 4 cut(s) 260, 676, 1206, 1542
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 3 cut(s) 73, 1191, 1335
Fnu4HI GCNGC 7 cut(s) 81, 184, 538, 545, 611, 977, 1354
FokI GGATG 2 cut(s) 29, 329
FriOI GRGCYC 4 cut(s) 261, 750, 1137, 1687
Fsp4HI GCNGC 7 cut(s) 81, 184, 538, 545, 611, 977, 1354
FspBI CTAG 4 cut(s) 102, 407, 614, 1359
GlaI GCGC 4 cut(s) 331, 881, 1253, 1357
GluI GCNGC 7 cut(s) 81, 184, 538, 545, 611, 977, 1354
GsaI CCCAGC 1 cut(s) 976
GsuI CTGGAG 1 cut(s) 965
HaeII RGCGCY 3 cut(s) 883, 1255, 1359
HapII CCGG 3 cut(s) 672, 918, 1206
HgaI GACGC 2 cut(s) 561, 953
HhaI GCGC 4 cut(s) 332, 882, 1254, 1358
Hin1I GRCGYC 4 cut(s) 553, 880, 1188, 1252
Hin1II CATG 8 cut(s) 437, 1129, 1150, 1240, 1331, 1468, 1516, 1528
Hin6I GCGC 4 cut(s) 330, 880, 1252, 1356
HinP1I GCGC 4 cut(s) 330, 880, 1252, 1356
HinfI GANTC 7 cut(s) 6, 296, 351, 476, 636, 675, 688
HpaII CCGG 3 cut(s) 672, 918, 1206
HphI GGTGA 6 cut(s) 78, 346, 614, 662, 670, 1384
Hpy166II GTNNAC 6 cut(s) 74, 412, 860, 1192, 1336, 1375
Hpy188I TCNGA 5 cut(s) 291, 348, 481, 490, 635
Hpy188III TCNNGA 3 cut(s) 461, 1495, 1688
Hpy8I GTNNAC 6 cut(s) 74, 412, 860, 1192, 1336, 1375
Hpy99I CGWCG 3 cut(s) 419, 947, 1190
HpyAV CCTTC 4 cut(s) 383, 641, 864, 1501
HpyCH4III ACNGT 7 cut(s) 71, 620, 701, 1053, 1110, 1595, 1636
HpyCH4IV ACGT 5 cut(s) 414, 1027, 1188, 1341, 1563
HpyCH4V TGCA 4 cut(s) 1078, 1093, 1150, 1456
HpyF10VI GCNNNNNNNGC 8 cut(s) 276, 338, 534, 579, 668, 754, 886, 1691
HpyF3I CTNAG 4 cut(s) 187, 207, 1247, 1262
HpySE526I ACGT 5 cut(s) 414, 1027, 1188, 1341, 1563
Hsp92I GRCGYC 4 cut(s) 553, 880, 1188, 1252
Hsp92II CATG 8 cut(s) 437, 1129, 1150, 1240, 1331, 1468, 1516, 1528
HspAI GCGC 4 cut(s) 330, 880, 1252, 1356
KasI GGCGCC 2 cut(s) 879, 1251
KroI GCCGGC 1 cut(s) 917
KroNI GCCGGC 1 cut(s) 919
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 117, 243, 291
LguI GCTCTTC 1 cut(s) 1142
LmnI GCTCC 5 cut(s) 453, 939, 984, 1132, 1674
Lsp1109I GCAGC 4 cut(s) 170, 556, 622, 1365
LweI GCATC 2 cut(s) 1221, 1681
MabI ACCWGGT 1 cut(s) 1404
MaeI CTAG 4 cut(s) 102, 407, 614, 1359
MaeII ACGT 5 cut(s) 414, 1027, 1188, 1341, 1563
MaeIII GTNAC 4 cut(s) 620, 676, 701, 1053
MalI GATC 3 cut(s) 119, 245, 293
MboI GATC 3 cut(s) 117, 243, 291
MboII GAAGA 6 cut(s) 166, 485, 1129, 1273, 1417, 1650
MflI RGATCY 1 cut(s) 243
MhlI GDGCHC 6 cut(s) 261, 450, 750, 1137, 1555, 1687
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 2 cut(s) 566, 776
MluNI TGGCCA 1 cut(s) 131
Mly113I GGCGCC 2 cut(s) 880, 1252
MlyI GAGTC 3 cut(s) 305, 682, 684
MmeI TCCRAC 1 cut(s) 269
Mox20I TGGCCA 1 cut(s) 131
MroNI GCCGGC 1 cut(s) 917
MscI TGGCCA 1 cut(s) 131
MseI TTAA 1 cut(s) 1696
MslI CAYNNNNRTG 2 cut(s) 125, 1151
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 2 cut(s) 235, 976
MspI CCGG 3 cut(s) 672, 918, 1206
MspR9I CCNGG 7 cut(s) 301, 314, 453, 877, 983, 1406, 1415
MvaI CCWGG 7 cut(s) 301, 314, 453, 877, 983, 1406, 1415
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 8 cut(s) 276, 338, 534, 579, 668, 754, 886, 1691
NaeI GCCGGC 1 cut(s) 919
NarI GGCGCC 2 cut(s) 880, 1252
NcoI CCATGG 1 cut(s) 1524
NdeII GATC 3 cut(s) 117, 243, 291
NgoMIV GCCGGC 1 cut(s) 917
NlaIII CATG 8 cut(s) 437, 1129, 1150, 1240, 1331, 1468, 1516, 1528
NlaIV GGNNCC 6 cut(s) 632, 881, 980, 1253, 1610, 1667
NmeAIII GCCGAG 1 cut(s) 1294
NmuCI GTSAC 3 cut(s) 620, 676, 1053
NspI RCATGY 2 cut(s) 1150, 1240
PaeI GCATGC 1 cut(s) 1240
PaqCI CACCTGC 1 cut(s) 689
PciSI GCTCTTC 1 cut(s) 1142
PcsI WCGNNNNNNNCGW 2 cut(s) 1038, 1383
PdiI GCCGGC 1 cut(s) 919
PfeI GAWTC 4 cut(s) 6, 351, 476, 636
PflFI GACNNNGTC 3 cut(s) 1037, 1406, 1565
PkrI GCNGC 7 cut(s) 82, 185, 539, 546, 612, 978, 1355
PleI GAGTC 3 cut(s) 304, 682, 683
PluTI GGCGCC 2 cut(s) 883, 1255
PpsI GAGTC 3 cut(s) 304, 682, 683
PpuMI RGGWCCY 1 cut(s) 1536
PshAI GACNNNNGTC 1 cut(s) 1541
Psp124BI GAGCTC 2 cut(s) 750, 1137
Psp5II RGGWCCY 1 cut(s) 1536
Psp6I CCWGG 7 cut(s) 299, 312, 451, 875, 981, 1404, 1413
PspEI GGTNACC 1 cut(s) 701
PspFI CCCAGC 1 cut(s) 972
PspGI CCWGG 7 cut(s) 299, 312, 451, 875, 981, 1404, 1413
PspN4I GGNNCC 6 cut(s) 632, 881, 980, 1253, 1610, 1667
PspPI GGNCC 8 cut(s) 505, 631, 873, 1408, 1536, 1567, 1591, 1665
PspPPI RGGWCCY 1 cut(s) 1536
PsrI GAACNNNNNNTAC 2 cut(s) 1601, 1633
PsuI RGATCY 1 cut(s) 243
PsyI GACNNNGTC 3 cut(s) 1037, 1406, 1565
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 3 cut(s) 26, 1484, 1619
RsaNI GTAC 3 cut(s) 25, 1483, 1618
RseI CAYNNNNRTG 2 cut(s) 125, 1151
SacI GAGCTC 2 cut(s) 750, 1137
SapI GCTCTTC 1 cut(s) 1142
SaqAI TTAA 1 cut(s) 1696
SatI GCNGC 7 cut(s) 81, 184, 538, 545, 611, 977, 1354
Sau3AI GATC 3 cut(s) 117, 243, 291
Sau96I GGNCC 8 cut(s) 505, 631, 873, 1408, 1536, 1567, 1591, 1665
SchI GAGTC 3 cut(s) 305, 682, 684
ScrFI CCNGG 7 cut(s) 301, 314, 453, 877, 983, 1406, 1415
SduI GDGCHC 6 cut(s) 261, 450, 750, 1137, 1555, 1687
SexAI ACCWGGT 1 cut(s) 1404
SfaNI GCATC 2 cut(s) 1221, 1681
SfcI CTRYAG 2 cut(s) 1192, 1300
SfiI GGCCNNNNNGGCC 2 cut(s) 534, 579
SfoI GGCGCC 2 cut(s) 881, 1253
SinI GGWCC 7 cut(s) 631, 873, 1408, 1536, 1567, 1591, 1665
SmiMI CAYNNNNRTG 2 cut(s) 125, 1151
SmlI CTYRAG 2 cut(s) 1613, 1651
SmoI CTYRAG 2 cut(s) 1613, 1651
SpeI ACTAGT 1 cut(s) 406
SphI GCATGC 1 cut(s) 1240
Sse9I AATT 2 cut(s) 566, 776
SspDI GGCGCC 2 cut(s) 879, 1251
SspMI CTAG 4 cut(s) 102, 407, 614, 1359
SstI GAGCTC 2 cut(s) 750, 1137
StyD4I CCNGG 7 cut(s) 299, 312, 451, 875, 981, 1404, 1413
StyI CCWWGG 3 cut(s) 421, 770, 1524
TaaI ACNGT 7 cut(s) 71, 620, 701, 1053, 1110, 1595, 1636
TaiI ACGT 5 cut(s) 417, 1030, 1191, 1344, 1566
TaqI TCGA 5 cut(s) 294, 924, 957, 1032, 1041
TaqII GACCGA 2 cut(s) 1514, 1653
TasI AATT 2 cut(s) 566, 776
TatI WGTACW 2 cut(s) 1482, 1617
TauI GCSGC 3 cut(s) 83, 540, 979
TfiI GAWTC 4 cut(s) 6, 351, 476, 636
Tru1I TTAA 1 cut(s) 1696
Tru9I TTAA 1 cut(s) 1696
TscAI CASTG 3 cut(s) 120, 332, 1316
TseFI GTSAC 3 cut(s) 620, 676, 1053
TseI GCWGC 4 cut(s) 183, 544, 610, 1353
Tsp45I GTSAC 3 cut(s) 620, 676, 1053
TspDTI ATGAA 5 cut(s) 1128, 1169, 1344, 1417, 1532
TspGWI ACGGA 2 cut(s) 1488, 1531
TspRI CASTG 3 cut(s) 120, 332, 1316
Tth111I GACNNNGTC 3 cut(s) 1037, 1406, 1565
VpaK11BI GGWCC 7 cut(s) 631, 873, 1408, 1536, 1567, 1591, 1665
XapI RAATTY 1 cut(s) 776
XceI RCATGY 2 cut(s) 1150, 1240
XmiI GTMKAC 3 cut(s) 73, 1191, 1335
XspI CTAG 4 cut(s) 102, 407, 614, 1359
ZraI GACGTC 1 cut(s) 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.