Rh6DG417000

Gamma carbonic anhydrase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0012857
Physical Location & Seq
Forward (+)
60037923 .. 60042435
4513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG417000.1

Sequence Viewer

Length: 621 bp
ATGGGGACTTTAGGAAGAGCGATATACACCGTCGGATTCTGGATTAGGGAGACCGGCCAGGCCGTCGATCGCCTCGGCAGCCGCCTCCAAGGAAGCTACTACTTCAAAGAGCAGCTGTCTAGGCATCGGACTCTTATGAATGTATTCGATAAAGCTCCTGTTGTTGACAAGGATGCATTTGTGGCCCCAAGTGCCTCCGTCATTGGTGATGTTCAAGTGGGAAGAGGATCTTCTATTTGGTATGGATGTGTATTGAGAGGTGATGTGAACCACATTGTTATTGGAGCTGGAACTAACATACAGGACAACTCCCTTGTGCATGTGGCAAAGTCTAATTTAAGTGGGAAGGTGTTGCCAACCATTATTGGTGATAATGTCACCGTAGGTCACAGTGCTGTTGTACATGGCTGTACGGTTGAGGATGAGGCCTTTGTTGGTATGGGAGCCACACTGCTTGATGGTGTCGTTGTTGAGAAACATGCTATGGTTGCTGCCGGAGCCCTTGTGAGACAGAATACAAGGATCCCCAGTGGAGAGGTATGGGCACCTACTGAACCCTCTTCTATATTTTGTTTATTTTCTCTTGGAAAGTATGATGATTTTAATAAGCATCCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hexapep

Protein Analysis

206

Amino Acids

22.1

Weight (kDa)

7.05

Isoelectric Point (pI)

25.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LbH_EIF2B PF25084 91 - 176 7.1e-07 EIF2B subunit epsilon LbH domain
Hexapep PF00132 119 - 151 6.7e-06 Bacterial transferase hexapeptide (six repeats)
Hexapep_2 PF14602 119 - 151 2.6e-06 Hexapeptide repeat of succinyl-transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47260
fragaria_vesca FvH4_2g39590
malus_domestica MD08G1029100.v1.1 MD15G1024600.v1.1
prunus_persica Prupe.1G376900_v2.0.a1 Prupe.1G376900_v2.0.a1
pyrus_communis pycom08g02290 pycom15g02120
rosa_chinensis RchiOBHm_Chr6g0301331
rosa_multiflora Rmu_sc0003197.1_g000011 Rmu_sc0013354.1_g000021
rosa_roxburghii Rroxscaffold_7G00166910
rosa_rugosa Rorug06G0303700 Rorug07G0031300
rosa_samantha Rh6BG423900 Rh6CG431100 Rh6DG417000
rosa_wichuraiana Rw6G036110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 544
AciI CCGC 1 cut(s) 82
AclWI GGATC 3 cut(s) 235, 517, 530
AcoI YGGCCR 1 cut(s) 55
AfaI GTAC 2 cut(s) 402, 412
AgsI TTSAA 2 cut(s) 106, 215
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 4 cut(s) 96, 115, 155, 287
AluI AGCT 4 cut(s) 96, 115, 155, 287
Alw26I GTCTC 2 cut(s) 44, 502
AlwI GGATC 3 cut(s) 235, 517, 530
AoxI GGCC 4 cut(s) 55, 60, 183, 426
ApeKI GCWGC 3 cut(s) 78, 112, 491
Asp700I GAANNNNTTC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 184
AsuHPI GGTGA 4 cut(s) 218, 272, 370, 380
BaeGI GKGCMC 1 cut(s) 547
BamHI GGATCC 1 cut(s) 522
BanI GGYRCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 502
BbvI GCAGC 3 cut(s) 90, 124, 478
BccI CCATC 1 cut(s) 452
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 59
BcoDI GTCTC 2 cut(s) 44, 502
BfaI CTAG 1 cut(s) 120
BisI GCNGC 4 cut(s) 79, 82, 113, 492
BlsI GCNGC 4 cut(s) 80, 83, 114, 493
Bme1390I CCNGG 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 5 cut(s) 186, 445, 499, 524, 546
BmrFI CCNGG 1 cut(s) 59
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 2 cut(s) 133, 163
BmuI ACTGGG 1 cut(s) 522
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 73, 88
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 528
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 2 cut(s) 73, 88
BseGI GGATG 4 cut(s) 178, 251, 427, 610
BseNI ACTGG 1 cut(s) 528
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 3 cut(s) 90, 124, 478
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 4 cut(s) 57, 62, 185, 428
BshNI GGYRCC 1 cut(s) 544
BsiEI CGRYCG 1 cut(s) 70
BsiSI CCGG 2 cut(s) 54, 495
BslFI GGGAC 1 cut(s) 19
BsmAI GTCTC 2 cut(s) 44, 502
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 4 cut(s) 57, 62, 185, 428
Bso31I GGTCTC 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 502, 547
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 3 cut(s) 67, 227, 522
BspACI CCGC 1 cut(s) 82
BspANI GGCC 4 cut(s) 57, 62, 185, 428
BspLI GGNNCC 5 cut(s) 186, 445, 499, 524, 546
BspPI GGATC 3 cut(s) 235, 517, 530
BspQI GCTCTTC 1 cut(s) 10
BspT107I GGYRCC 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 44
BsrFI RCCGGY 1 cut(s) 53
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 1 cut(s) 528
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 2 cut(s) 73, 88
BssMI GATC 3 cut(s) 67, 227, 522
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 4 cut(s) 31, 382, 392, 415
Bst6I CTCTTC 3 cut(s) 10, 217, 565
BstAUI TGTACA 1 cut(s) 400
BstF5I GGATG 4 cut(s) 178, 251, 427, 610
BstKTI GATC 3 cut(s) 70, 230, 525
BstMAI GTCTC 2 cut(s) 44, 502
BstMBI GATC 3 cut(s) 67, 227, 522
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 6 cut(s) 78, 121, 182, 191, 488, 497
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 2 cut(s) 323, 482
BstSCI CCNGG 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 547
BstV1I GCAGC 3 cut(s) 90, 124, 478
BstX2I RGATCY 2 cut(s) 227, 522
BstYI RGATCY 2 cut(s) 227, 522
BsuRI GGCC 4 cut(s) 57, 62, 185, 428
BtsCI GGATG 4 cut(s) 178, 251, 427, 610
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 3 cut(s) 397, 449, 535
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 401, 411
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 3 cut(s) 320, 404, 479
CviQI GTAC 2 cut(s) 401, 411
DpnI GATC 3 cut(s) 69, 229, 524
DpnII GATC 3 cut(s) 67, 227, 522
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 3 cut(s) 10, 217, 565
EarI CTCTTC 3 cut(s) 10, 217, 565
Eco130I CCWWGG 1 cut(s) 88
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 502
Eco31I GGTCTC 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 88
EcoT22I ATGCAT 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 3 cut(s) 323, 407, 482
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 19
FatI CATG 3 cut(s) 319, 403, 478
Fnu4HI GCNGC 4 cut(s) 79, 82, 113, 492
FokI GGATG 4 cut(s) 185, 258, 434, 597
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 4 cut(s) 79, 82, 113, 492
FspBI CTAG 1 cut(s) 120
GluI GCNGC 4 cut(s) 79, 82, 113, 492
HaeIII GGCC 4 cut(s) 57, 62, 185, 428
HapII CCGG 2 cut(s) 54, 495
Hin1II CATG 3 cut(s) 323, 407, 482
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 2 cut(s) 36, 130
HpaII CCGG 2 cut(s) 54, 495
HphI GGTGA 4 cut(s) 218, 272, 370, 380
Hpy166II GTNNAC 2 cut(s) 166, 268
Hpy188I TCNGA 2 cut(s) 35, 129
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 2 cut(s) 166, 268
Hpy99I CGWCG 2 cut(s) 35, 68
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 4 cut(s) 31, 382, 392, 415
HpyCH4V TGCA 2 cut(s) 176, 319
HpyF10VI GCNNNNNNNGC 6 cut(s) 78, 121, 182, 191, 488, 497
Hsp92II CATG 3 cut(s) 323, 407, 482
Kzo9I GATC 3 cut(s) 67, 227, 522
LguI GCTCTTC 1 cut(s) 10
LmnI GCTCC 4 cut(s) 160, 284, 443, 497
LpnPI CCDG 9 cut(s) 25, 44, 67, 71, 171, 273, 287, 508, 541
Lsp1109I GCAGC 3 cut(s) 90, 124, 478
LweI GCATC 2 cut(s) 133, 163
MaeI CTAG 1 cut(s) 120
MaeIII GTNAC 2 cut(s) 376, 386
MalI GATC 3 cut(s) 69, 229, 524
MboI GATC 3 cut(s) 67, 227, 522
MboII GAAGA 4 cut(s) 27, 222, 234, 552
MflI RGATCY 2 cut(s) 227, 522
MhlI GDGCHC 2 cut(s) 502, 547
MluCI AATT 1 cut(s) 334
MlyI GAGTC 1 cut(s) 124
MmeI TCCRAC 1 cut(s) 13
MnlI CCTC 9 cut(s) 83, 95, 205, 218, 251, 412, 418, 529, 568
Mph1103I ATGCAT 1 cut(s) 178
MroXI GAANNNNTTC 1 cut(s) 143
MseI TTAA 2 cut(s) 338, 603
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 2 cut(s) 54, 495
MspR9I CCNGG 1 cut(s) 59
MvaI CCWGG 1 cut(s) 59
MwoI GCNNNNNNNGC 6 cut(s) 78, 121, 182, 191, 488, 497
NdeII GATC 3 cut(s) 67, 227, 522
NlaIII CATG 3 cut(s) 323, 407, 482
NlaIV GGNNCC 5 cut(s) 186, 445, 499, 524, 546
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 2 cut(s) 376, 386
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 2 cut(s) 323, 482
PceI AGGCCT 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 36
PkrI GCNGC 4 cut(s) 80, 83, 114, 493
Ple19I CGATCG 1 cut(s) 70
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 5 cut(s) 186, 445, 499, 524, 546
PspPI GGNCC 1 cut(s) 184
PsuI RGATCY 2 cut(s) 227, 522
PvuI CGATCG 1 cut(s) 70
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 2 cut(s) 402, 412
RsaNI GTAC 2 cut(s) 401, 411
SapI GCTCTTC 1 cut(s) 10
SaqAI TTAA 2 cut(s) 338, 603
SatI GCNGC 4 cut(s) 79, 82, 113, 492
Sau3AI GATC 3 cut(s) 67, 227, 522
Sau96I GGNCC 1 cut(s) 184
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 2 cut(s) 502, 547
SetI ASST 9 cut(s) 98, 117, 157, 262, 289, 351, 388, 540, 550
SfaNI GCATC 2 cut(s) 133, 163
Sse9I AATT 1 cut(s) 334
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 1 cut(s) 82
SspMI CTAG 1 cut(s) 120
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 4 cut(s) 31, 382, 392, 415
TaqI TCGA 2 cut(s) 66, 147
TasI AATT 1 cut(s) 334
TatI WGTACW 1 cut(s) 400
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 1 cut(s) 36
Tru1I TTAA 2 cut(s) 338, 603
Tru9I TTAA 2 cut(s) 338, 603
TscAI CASTG 3 cut(s) 397, 456, 535
TseFI GTSAC 2 cut(s) 376, 386
TseI GCWGC 3 cut(s) 78, 112, 491
Tsp45I GTSAC 2 cut(s) 376, 386
TspDTI ATGAA 1 cut(s) 152
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 3 cut(s) 397, 456, 535
XceI RCATGY 2 cut(s) 323, 482
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.