pycom08g02290

Gamma carbonic anhydrase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012857
Physical Location & Seq
Reverse (-)
1708578 .. 1711730
3153 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g02290.4

Sequence Viewer

Length: 819 bp
ATGGGGACTTTAGGACGAGCAATATACACCGTCGGATTCTGGATTCGGGAGACGGGCCAGGCCATTGATCGCCTCGGCAGCAGCCTCCAGGGCAGCTACTACTTCAAAGAGCAGCTGTCTCGACATAGAACTCTGATGAACGTATTTGATAAAGCTCCTGTGGTTGACAAAGATGTGTTTGTGGCGCCAAGTGCCTCTGTCATTGGCGATGTTCAAGTGGGAAGAGGGTCTTCCATTTGGTATGGATGTGTTTTGAGAGGTGATGTGAACAACATCGTTGTTGGATCTCGAACCAACATACAGGACAACTCCCTTGTTCATGTGGCGAAGTCTAATTTAAGCGGGAAGGTGTTACCAACTATTATTGGAGACAATGTTACTGTAGGTCACAGTGCCGTTATACATGGCTGTACTGTTGAGGATGAGGCCTTTGTTGGTATGGGAGCCACTCTTCTTGATGGTGTCGTTGTTGAGAAACATGCCATGGTAGCTGCTGGATCCCTGGTGAGACAGAATACAAGGATCCCTAGTGGAGAGGTATGGGCAGGAAACCCAGCAAAATTCCTGAGGAAGCTCACAGATGAAGAAAAAGCCTTCATCTCCCAGTCAGCCACCAATTACACTAACCTTGCACAAGTCCATGCAGCTGAGAATGTGAAGTCGTTTGATGAGATTGAGTTTGAGAAGGCCCTCCGCAAGAAGTATGCACATAAGGATGAGGAGTACGACTCAATGCTGGGTGTTGTTCGTGAGATTCCCCCAGAACTTATTCTTCCCGATAATGTATTACCAGATAAAGCAGCAAAGGACCTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hexapep

Protein Analysis

273

Amino Acids

29.64

Weight (kDa)

6.18

Isoelectric Point (pI)

31.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47260
fragaria_vesca FvH4_2g39590
malus_domestica MD08G1029100.v1.1 MD15G1024600.v1.1
prunus_persica Prupe.1G376900_v2.0.a1 Prupe.1G376900_v2.0.a1
pyrus_communis pycom08g02290 pycom15g02120
rosa_chinensis RchiOBHm_Chr6g0301331
rosa_multiflora Rmu_sc0003197.1_g000011 Rmu_sc0013354.1_g000021
rosa_roxburghii Rroxscaffold_7G00166910
rosa_rugosa Rorug06G0303700 Rorug07G0031300
rosa_samantha Rh6BG423900 Rh6CG431100 Rh6DG417000
rosa_wichuraiana Rw6G036110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AciI CCGC 2 cut(s) 342, 694
AclWI GGATC 5 cut(s) 292, 492, 505, 517, 530
AcsI RAATTY 1 cut(s) 560
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 2 cut(s) 412, 725
AgsI TTSAA 2 cut(s) 106, 215
AjnI CCWGG 3 cut(s) 57, 87, 501
AluBI AGCT 6 cut(s) 96, 115, 155, 491, 574, 647
AluI AGCT 6 cut(s) 96, 115, 155, 491, 574, 647
Alw26I GTCTC 4 cut(s) 44, 123, 363, 502
AlwI GGATC 5 cut(s) 292, 492, 505, 517, 530
AoxI GGCC 4 cut(s) 55, 60, 426, 687
ApeKI GCWGC 7 cut(s) 78, 81, 93, 112, 491, 644, 800
ApoI RAATTY 1 cut(s) 560
Asp700I GAANNNNTTC 1 cut(s) 768
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 3 cut(s) 55, 688, 808
AsuHPI GGTGA 2 cut(s) 272, 517
AvaII GGWCC 1 cut(s) 808
AxyI CCTNAGG 1 cut(s) 566
BamHI GGATCC 2 cut(s) 497, 522
BanI GGYRCC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 7 cut(s) 90, 93, 105, 124, 478, 656, 812
BccI CCATC 1 cut(s) 452
BceAI ACGGC 1 cut(s) 380
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 3 cut(s) 59, 89, 503
BcoDI GTCTC 4 cut(s) 44, 123, 363, 502
BfaI CTAG 1 cut(s) 528
BfmI CTRYAG 1 cut(s) 381
BfoI RGCGCY 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 90
BisI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
BlsI GCNGC 7 cut(s) 80, 83, 95, 114, 493, 646, 802
Bme1390I CCNGG 3 cut(s) 59, 89, 503
Bme18I GGWCC 1 cut(s) 808
BmgT120I GGNCC 3 cut(s) 55, 688, 808
BmiI GGNNCC 4 cut(s) 186, 445, 499, 524
BmrFI CCNGG 3 cut(s) 59, 89, 503
BmrI ACTGGG 1 cut(s) 598
BmuI ACTGGG 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 222
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpmI CTGGAG 1 cut(s) 71
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 4 cut(s) 73, 88, 483, 501
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bse1I ACTGG 1 cut(s) 604
Bse21I CCTNAGG 1 cut(s) 566
BseBI CCWGG 3 cut(s) 59, 89, 503
BseDI CCNNGG 4 cut(s) 73, 88, 483, 501
BseGI GGATG 3 cut(s) 251, 427, 721
BseMII CTCAG 2 cut(s) 557, 639
BseNI ACTGG 1 cut(s) 604
BseRI GAGGAG 1 cut(s) 734
BseXI GCAGC 7 cut(s) 90, 93, 105, 124, 478, 656, 812
BseYI CCCAGC 2 cut(s) 553, 736
BshFI GGCC 4 cut(s) 57, 62, 428, 689
BshNI GGYRCC 1 cut(s) 184
BslFI GGGAC 1 cut(s) 19
BsmAI GTCTC 4 cut(s) 44, 123, 363, 502
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 4 cut(s) 57, 62, 428, 689
Bsp143I GATC 4 cut(s) 67, 284, 497, 522
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 2 cut(s) 342, 694
BspANI GGCC 4 cut(s) 57, 62, 428, 689
BspCNI CTCAG 2 cut(s) 558, 640
BspLI GGNNCC 4 cut(s) 186, 445, 499, 524
BspPI GGATC 5 cut(s) 292, 492, 505, 517, 530
BspT107I GGYRCC 1 cut(s) 184
BsrI ACTGG 1 cut(s) 604
BssECI CCNNGG 4 cut(s) 73, 88, 483, 501
BssMI GATC 4 cut(s) 67, 284, 497, 522
BssNI GRCGYC 1 cut(s) 185
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 3 cut(s) 59, 89, 503
Bst4CI ACNGT 4 cut(s) 31, 382, 392, 415
Bst6I CTCTTC 2 cut(s) 217, 456
BstACI GRCGYC 1 cut(s) 185
BstDEI CTNAG 2 cut(s) 566, 648
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 3 cut(s) 251, 427, 721
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 4 cut(s) 70, 287, 500, 525
BstMAI GTCTC 4 cut(s) 44, 123, 363, 502
BstMBI GATC 4 cut(s) 67, 284, 497, 522
BstMWI GCNNNNNNNGC 4 cut(s) 78, 90, 191, 488
BstNI CCWGG 3 cut(s) 59, 89, 503
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 3 cut(s) 57, 87, 501
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 7 cut(s) 90, 93, 105, 124, 478, 656, 812
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 3 cut(s) 284, 497, 522
BstYI RGATCY 3 cut(s) 284, 497, 522
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 4 cut(s) 57, 62, 428, 689
BtgI CCRYGG 1 cut(s) 483
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 3 cut(s) 251, 427, 721
BtsIMutI CAGTG 1 cut(s) 397
CfoI GCGC 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 55, 688, 808
Csp6I GTAC 2 cut(s) 411, 724
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 5 cut(s) 320, 404, 479, 484, 641
CviQI GTAC 2 cut(s) 411, 724
DdeI CTNAG 2 cut(s) 566, 648
DinI GGCGCC 1 cut(s) 186
DpnI GATC 4 cut(s) 69, 286, 499, 524
DpnII GATC 4 cut(s) 67, 284, 497, 522
Eam1104I CTCTTC 2 cut(s) 217, 456
EarI CTCTTC 2 cut(s) 217, 456
Eco130I CCWWGG 1 cut(s) 483
Eco147I AGGCCT 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 808
Eco81I CCTNAGG 1 cut(s) 566
EcoO109I RGGNCCY 2 cut(s) 688, 808
EcoRII CCWGG 3 cut(s) 57, 87, 501
EcoT14I CCWWGG 1 cut(s) 483
EgeI GGCGCC 1 cut(s) 186
EheI GGCGCC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 5 cut(s) 323, 407, 482, 487, 644
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 19
FatI CATG 5 cut(s) 319, 403, 478, 483, 640
FauI CCCGC 1 cut(s) 335
Fnu4HI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
FokI GGATG 3 cut(s) 258, 434, 728
Fsp4HI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
FspBI CTAG 1 cut(s) 528
GlaI GCGC 1 cut(s) 186
GluI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
GsaI CCCAGC 2 cut(s) 557, 740
GsuI CTGGAG 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 4 cut(s) 57, 62, 428, 689
HhaI GCGC 1 cut(s) 187
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 5 cut(s) 323, 407, 482, 487, 644
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 4 cut(s) 36, 43, 728, 754
HphI GGTGA 2 cut(s) 272, 517
Hpy166II GTNNAC 2 cut(s) 166, 268
Hpy188I TCNGA 2 cut(s) 35, 135
Hpy188III TCNNGA 8 cut(s) 40, 47, 120, 288, 455, 565, 749, 776
Hpy8I GTNNAC 2 cut(s) 166, 268
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 3 cut(s) 340, 604, 679
HpyCH4III ACNGT 4 cut(s) 31, 382, 392, 415
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 3 cut(s) 632, 644, 707
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 90, 191, 488
HpyF3I CTNAG 2 cut(s) 566, 648
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 5 cut(s) 323, 407, 482, 487, 644
HspAI GCGC 1 cut(s) 185
KasI GGCGCC 1 cut(s) 184
Kzo9I GATC 4 cut(s) 67, 284, 497, 522
LmnI GCTCC 2 cut(s) 160, 443
Lsp1109I GCAGC 7 cut(s) 90, 93, 105, 124, 478, 656, 812
MaeI CTAG 1 cut(s) 528
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 351, 376, 386
MalI GATC 4 cut(s) 69, 286, 499, 524
MboI GATC 4 cut(s) 67, 284, 497, 522
MboII GAAGA 5 cut(s) 222, 234, 443, 596, 764
MflI RGATCY 3 cut(s) 284, 497, 522
MluCI AATT 3 cut(s) 334, 560, 616
Mly113I GGCGCC 1 cut(s) 185
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 2 cut(s) 13, 262
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 714
MspA1I CMGCKG 2 cut(s) 115, 647
MspR9I CCNGG 3 cut(s) 59, 89, 503
MvaI CCWGG 3 cut(s) 59, 89, 503
MwoI GCNNNNNNNGC 4 cut(s) 78, 90, 191, 488
NarI GGCGCC 1 cut(s) 185
NcoI CCATGG 1 cut(s) 483
NdeII GATC 4 cut(s) 67, 284, 497, 522
NlaIII CATG 5 cut(s) 323, 407, 482, 487, 644
NlaIV GGNNCC 4 cut(s) 186, 445, 499, 524
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 1 cut(s) 386
NspI RCATGY 1 cut(s) 482
PceI AGGCCT 1 cut(s) 428
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 3 cut(s) 36, 43, 754
PkrI GCNGC 7 cut(s) 80, 83, 95, 114, 493, 646, 802
PleI GAGTC 1 cut(s) 722
PluTI GGCGCC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 722
PpuMI RGGWCCY 1 cut(s) 808
Psp5II RGGWCCY 1 cut(s) 808
Psp6I CCWGG 3 cut(s) 57, 87, 501
PspFI CCCAGC 2 cut(s) 553, 736
PspGI CCWGG 3 cut(s) 57, 87, 501
PspN4I GGNNCC 4 cut(s) 186, 445, 499, 524
PspPI GGNCC 3 cut(s) 55, 688, 808
PspPPI RGGWCCY 1 cut(s) 808
PsuI RGATCY 3 cut(s) 284, 497, 522
PvuII CAGCTG 2 cut(s) 115, 647
RsaI GTAC 2 cut(s) 412, 725
RsaNI GTAC 2 cut(s) 411, 724
RseI CAYNNNNRTG 1 cut(s) 714
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 7 cut(s) 79, 82, 94, 113, 492, 645, 801
Sau3AI GATC 4 cut(s) 67, 284, 497, 522
Sau96I GGNCC 3 cut(s) 55, 688, 808
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 3 cut(s) 59, 89, 503
SfcI CTRYAG 1 cut(s) 381
SfoI GGCGCC 1 cut(s) 186
SinI GGWCC 1 cut(s) 808
SmiMI CAYNNNNRTG 1 cut(s) 714
Sse9I AATT 3 cut(s) 334, 560, 616
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 2 cut(s) 342, 694
SspDI GGCGCC 1 cut(s) 184
SspMI CTAG 1 cut(s) 528
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 57, 87, 501
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 4 cut(s) 31, 382, 392, 415
TaiI ACGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 121, 289
TasI AATT 3 cut(s) 334, 560, 616
TatI WGTACW 1 cut(s) 410
TfiI GAWTC 3 cut(s) 36, 43, 754
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 1 cut(s) 397
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 7 cut(s) 78, 81, 93, 112, 491, 644, 800
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 4 cut(s) 152, 308, 586, 597
TspRI CASTG 1 cut(s) 397
VpaK11BI GGWCC 1 cut(s) 808
XapI RAATTY 1 cut(s) 560
XceI RCATGY 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.