Rw6G036110

Gamma carbonic anhydrase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012857
Physical Location & Seq
Forward (+)
60663593 .. 60666469
2877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G036110.1

Sequence Viewer

Length: 819 bp
ATGGGGACTTTAGGAAGAGCGATATACACCGTCGGATTCTGGATTAGGGAGACCGGCCAGGCCGTCGATCGCCTCGGCAGCCGCCTCCAAGGAAGCTACTACTTCAAGGAGCAGCTGTCTAGGCATCGGACTCTTATGAATGTATTCGATAAAGCTCCTGTTGTTGACAAGGATGCATTTGTGGCCCCAAGTGCCTCCGTCATTGGTGATGTTCAAGTGGGAAGAGGATCTTCTATTTGGTATGGATGTGTATTGAGAGGTGATGTGAACCACATTGTTATTGGAGCTGGAACTAACATACAGGACAACTCCCTTGTGCATGTGGCAAAGTCTAATTTAAGTGGGAAGGTGTTGCCAACCATTATTGGTGATAATGTCACCGTAGGTCACAGTGCTGTTGTACATGGCTGTACGGTTGAAGATGAGGCCTTTGTTGGTATGGGAGCCACACTGCTTGATGGTGTCGTTGTTGAGAAACATGCTATGGTTGCTGCCGGAGCCCTTGTGAGACAGAATACAAGGATCCCCAGTGGAGAGGTATGGGCAGGGAATCCAGCTAAGTTCCTGAGGAAGCTCACAGATGAAGAAATAGCCTTCATCTCCCAGTCAGCCACTAATTATGTTAACCTTGCACAAGTCCATGCGGCTGAGAATGGGAAGTCCTTTGATGAGATTGAGTTTGAGAAGGCCCTTCGTAAGAAGTTTGCTCGTCGTGATGAGGAGTATGACTCAATGCTGGGTGTTGTTCGTGAAATTCCCCCAGAACTTATTCTTCCAGATAATGTATTACCAGATAAAGCACTCAAGGCTGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hexapep

Protein Analysis

272

Amino Acids

29.55

Weight (kDa)

6.18

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LbH_EIF2B PF25084 91 - 176 1.3e-06 EIF2B subunit epsilon LbH domain
Hexapep PF00132 119 - 151 1e-05 Bacterial transferase hexapeptide (six repeats)
Hexapep_2 PF14602 119 - 151 3.9e-06 Hexapeptide repeat of succinyl-transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47260
fragaria_vesca FvH4_2g39590
malus_domestica MD08G1029100.v1.1 MD15G1024600.v1.1
prunus_persica Prupe.1G376900_v2.0.a1 Prupe.1G376900_v2.0.a1
pyrus_communis pycom08g02290 pycom15g02120
rosa_chinensis RchiOBHm_Chr6g0301331
rosa_multiflora Rmu_sc0003197.1_g000011 Rmu_sc0013354.1_g000021
rosa_roxburghii Rroxscaffold_7G00166910
rosa_rugosa Rorug06G0303700 Rorug07G0031300
rosa_samantha Rh6BG423900 Rh6CG431100 Rh6DG417000
rosa_wichuraiana Rw6G036110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 82, 644
AclWI GGATC 3 cut(s) 235, 517, 530
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 1 cut(s) 753
AfaI GTAC 2 cut(s) 402, 412
AgsI TTSAA 3 cut(s) 106, 215, 419
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 6 cut(s) 96, 115, 155, 287, 557, 574
AluI AGCT 6 cut(s) 96, 115, 155, 287, 557, 574
Alw26I GTCTC 2 cut(s) 44, 502
AlwI GGATC 3 cut(s) 235, 517, 530
AoxI GGCC 5 cut(s) 55, 60, 183, 426, 687
ApeKI GCWGC 3 cut(s) 78, 112, 491
ApoI RAATTY 1 cut(s) 753
Asp700I GAANNNNTTC 2 cut(s) 143, 768
AspS9I GGNCC 2 cut(s) 184, 688
AsuHPI GGTGA 4 cut(s) 218, 272, 370, 380
AxyI CCTNAGG 1 cut(s) 566
BamHI GGATCC 1 cut(s) 522
BanII GRGCYC 1 cut(s) 502
BbvI GCAGC 3 cut(s) 90, 124, 478
BccI CCATC 1 cut(s) 452
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 59
BcoDI GTCTC 2 cut(s) 44, 502
BfaI CTAG 1 cut(s) 120
BisI GCNGC 5 cut(s) 79, 82, 113, 492, 645
BlsI GCNGC 5 cut(s) 80, 83, 114, 493, 646
Bme1390I CCNGG 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 184, 688
BmiI GGNNCC 4 cut(s) 186, 445, 499, 524
BmrFI CCNGG 1 cut(s) 59
BmrI ACTGGG 2 cut(s) 522, 598
BmsI GCATC 2 cut(s) 133, 163
BmuI ACTGGG 2 cut(s) 522, 598
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpuEI CTTGAG 1 cut(s) 788
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 73, 88
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 489, 519
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 528, 604
Bse21I CCTNAGG 1 cut(s) 566
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 2 cut(s) 73, 88
BseGI GGATG 2 cut(s) 178, 251
BseMII CTCAG 2 cut(s) 557, 639
BseNI ACTGG 2 cut(s) 528, 604
BseRI GAGGAG 1 cut(s) 734
BseXI GCAGC 3 cut(s) 90, 124, 478
BseYI CCCAGC 1 cut(s) 736
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 5 cut(s) 57, 62, 185, 428, 689
BsiEI CGRYCG 1 cut(s) 70
BsiSI CCGG 2 cut(s) 54, 495
BslFI GGGAC 1 cut(s) 19
BsmAI GTCTC 2 cut(s) 44, 502
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 5 cut(s) 57, 62, 185, 428, 689
Bso31I GGTCTC 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 502
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 3 cut(s) 67, 227, 522
BspACI CCGC 2 cut(s) 82, 644
BspANI GGCC 5 cut(s) 57, 62, 185, 428, 689
BspCNI CTCAG 2 cut(s) 558, 640
BspLI GGNNCC 4 cut(s) 186, 445, 499, 524
BspPI GGATC 3 cut(s) 235, 517, 530
BspQI GCTCTTC 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 44
BsrFI RCCGGY 1 cut(s) 53
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 2 cut(s) 528, 604
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 2 cut(s) 73, 88
BssMI GATC 3 cut(s) 67, 227, 522
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 4 cut(s) 31, 382, 392, 415
Bst6I CTCTTC 2 cut(s) 10, 217
BstAUI TGTACA 1 cut(s) 400
BstDEI CTNAG 3 cut(s) 558, 566, 648
BstF5I GGATG 2 cut(s) 178, 251
BstKTI GATC 3 cut(s) 70, 230, 525
BstMAI GTCTC 2 cut(s) 44, 502
BstMBI GATC 3 cut(s) 67, 227, 522
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 7 cut(s) 78, 121, 182, 191, 488, 497, 806
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 2 cut(s) 323, 482
BstSCI CCNGG 1 cut(s) 57
BstV1I GCAGC 3 cut(s) 90, 124, 478
BstX2I RGATCY 2 cut(s) 227, 522
BstYI RGATCY 2 cut(s) 227, 522
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 5 cut(s) 57, 62, 185, 428, 689
BtsCI GGATG 2 cut(s) 178, 251
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 3 cut(s) 397, 449, 535
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 184, 688
Csp6I GTAC 2 cut(s) 401, 411
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 4 cut(s) 320, 404, 479, 641
CviQI GTAC 2 cut(s) 401, 411
DdeI CTNAG 3 cut(s) 558, 566, 648
DpnI GATC 3 cut(s) 69, 229, 524
DpnII GATC 3 cut(s) 67, 227, 522
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 10, 217
EarI CTCTTC 2 cut(s) 10, 217
Eco130I CCWWGG 1 cut(s) 88
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 502
Eco31I GGTCTC 1 cut(s) 44
Eco81I CCTNAGG 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 688
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 88
EcoT22I ATGCAT 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 4 cut(s) 323, 407, 482, 644
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 19
FatI CATG 4 cut(s) 319, 403, 478, 640
Fnu4HI GCNGC 5 cut(s) 79, 82, 113, 492, 645
FokI GGATG 2 cut(s) 185, 258
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 5 cut(s) 79, 82, 113, 492, 645
FspBI CTAG 1 cut(s) 120
GluI GCNGC 5 cut(s) 79, 82, 113, 492, 645
GsaI CCCAGC 1 cut(s) 740
HaeIII GGCC 5 cut(s) 57, 62, 185, 428, 689
HapII CCGG 2 cut(s) 54, 495
Hin1II CATG 4 cut(s) 323, 407, 482, 644
HincII GTYRAC 2 cut(s) 166, 625
HindII GTYRAC 2 cut(s) 166, 625
HinfI GANTC 4 cut(s) 36, 130, 550, 728
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 2 cut(s) 54, 495
HphI GGTGA 4 cut(s) 218, 272, 370, 380
Hpy166II GTNNAC 3 cut(s) 166, 268, 625
Hpy188I TCNGA 2 cut(s) 35, 129
Hpy188III TCNNGA 5 cut(s) 40, 565, 713, 749, 776
Hpy8I GTNNAC 3 cut(s) 166, 268, 625
Hpy99I CGWCG 3 cut(s) 35, 68, 714
HpyAV CCTTC 4 cut(s) 340, 604, 679, 701
HpyCH4III ACNGT 4 cut(s) 31, 382, 392, 415
HpyCH4V TGCA 3 cut(s) 176, 319, 632
HpyF10VI GCNNNNNNNGC 7 cut(s) 78, 121, 182, 191, 488, 497, 806
HpyF3I CTNAG 3 cut(s) 558, 566, 648
Hsp92II CATG 4 cut(s) 323, 407, 482, 644
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 3 cut(s) 67, 227, 522
LguI GCTCTTC 1 cut(s) 10
LmnI GCTCC 5 cut(s) 109, 160, 284, 443, 497
Lsp1109I GCAGC 3 cut(s) 90, 124, 478
LweI GCATC 2 cut(s) 133, 163
MaeI CTAG 1 cut(s) 120
MaeIII GTNAC 2 cut(s) 376, 386
MalI GATC 3 cut(s) 69, 229, 524
MboI GATC 3 cut(s) 67, 227, 522
MboII GAAGA 6 cut(s) 27, 222, 234, 431, 596, 764
MflI RGATCY 2 cut(s) 227, 522
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 3 cut(s) 334, 616, 753
MlyI GAGTC 2 cut(s) 124, 722
MmeI TCCRAC 1 cut(s) 13
MnlI CCTC 9 cut(s) 83, 95, 205, 218, 251, 418, 529, 561, 712
Mph1103I ATGCAT 1 cut(s) 178
MroXI GAANNNNTTC 2 cut(s) 143, 768
MseI TTAA 2 cut(s) 338, 624
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 2 cut(s) 54, 495
MspR9I CCNGG 1 cut(s) 59
MvaI CCWGG 1 cut(s) 59
MwoI GCNNNNNNNGC 7 cut(s) 78, 121, 182, 191, 488, 497, 806
NdeII GATC 3 cut(s) 67, 227, 522
NlaIII CATG 4 cut(s) 323, 407, 482, 644
NlaIV GGNNCC 4 cut(s) 186, 445, 499, 524
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 2 cut(s) 376, 386
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 2 cut(s) 323, 482
PceI AGGCCT 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 10
PdmI GAANNNNTTC 2 cut(s) 143, 768
PfeI GAWTC 2 cut(s) 36, 550
PkrI GCNGC 5 cut(s) 80, 83, 114, 493, 646
Ple19I CGATCG 1 cut(s) 70
PleI GAGTC 2 cut(s) 124, 722
PpsI GAGTC 2 cut(s) 124, 722
Psp6I CCWGG 1 cut(s) 57
PspFI CCCAGC 1 cut(s) 736
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 4 cut(s) 186, 445, 499, 524
PspPI GGNCC 2 cut(s) 184, 688
PsuI RGATCY 2 cut(s) 227, 522
PvuI CGATCG 1 cut(s) 70
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 2 cut(s) 402, 412
RsaNI GTAC 2 cut(s) 401, 411
SapI GCTCTTC 1 cut(s) 10
SaqAI TTAA 2 cut(s) 338, 624
SatI GCNGC 5 cut(s) 79, 82, 113, 492, 645
Sau3AI GATC 3 cut(s) 67, 227, 522
Sau96I GGNCC 2 cut(s) 184, 688
SchI GAGTC 2 cut(s) 124, 722
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 2 cut(s) 133, 163
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
Sse9I AATT 3 cut(s) 334, 616, 753
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 2 cut(s) 82, 644
SspMI CTAG 1 cut(s) 120
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 4 cut(s) 31, 382, 392, 415
TaqI TCGA 2 cut(s) 66, 147
TasI AATT 3 cut(s) 334, 616, 753
TatI WGTACW 1 cut(s) 400
TauI GCSGC 2 cut(s) 84, 647
TfiI GAWTC 2 cut(s) 36, 550
Tru1I TTAA 2 cut(s) 338, 624
Tru9I TTAA 2 cut(s) 338, 624
TscAI CASTG 3 cut(s) 397, 456, 535
TseFI GTSAC 2 cut(s) 376, 386
TseI GCWGC 3 cut(s) 78, 112, 491
Tsp45I GTSAC 2 cut(s) 376, 386
TspDTI ATGAA 3 cut(s) 152, 586, 597
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 3 cut(s) 397, 456, 535
XapI RAATTY 1 cut(s) 753
XceI RCATGY 2 cut(s) 323, 482
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XmnI GAANNNNTTC 2 cut(s) 143, 768
XspI CTAG 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.