FvH4_2g39940

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015873
Physical Location & Seq
Reverse (-)
28572686 .. 28574803
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39940.t1

Sequence Viewer

Length: 1134 bp
ATGAGGAAGTTGTGCCCTAACCTTGACAATGAAGATGGCCTTGATACCGTTCTCGAGGTGCCGATACCCGAAGAAATGTTCTCCCTTATGGGCAGCACCGCCGCATTGCGGTGGCATAACTTGCGTGCATTCATGAAAAATCAATCCCTTAATAAGCCATCCACATCTCATTTTCCGGCAGGGGTAAATAGTGAGTTTATGGCATTGCTTAAGCTTGTTGGTTCTCCTCTAATCCCTCTTCAGGTTCATTGCGACAATGGCCTCACGCGCCCCATCAGAGATTGTTCCATCGAGGCTTCTACCGCGAAGTACATTGTACAACAATATGTAGCAGCTATCGGCGGACTAGCGGCGTTGAACGCCGTGAATAGTATGTGTGCAGTAGGGCAGGTGAGGATGGTGGGATCTGAAATGCACAAAGGCGATGATAACAATGTTTATTCGACGACGACGAAAGTGAACAGTGAAGTTGGTGGATTTGTTCTTTGGCAAAAGAATCCCGACTTGTGGTACTTAGAACTGGTTGTTTCCGGCTACAAGGTAAGCGCCGGAAGTGATGGCGCCGTCGCATGGAATCAGTCCGGTTCTCAACCTTCTCATGCCAATAAAGGCCCTCCAAGACCCCTTCGTCGCTTCTTTCAGGGATTGGATCCGAGGAGCACGGCGAACTTGTTTTTGGATGCAGTATGCACCGGAGAGAAAACCATCAACAAAGAAGAATGTTTCATACTCAAGCTTGAAACCCCAGCAGATATTCTTCAATCGCAAAGCACATCCCAAACTGAAATAGTCCACCATACCATATGGGGGTACTTCAGCCAAAGAACAGGGCTTCTTGTCCAGTTTGAGGACACAAAGCTGGTGAAGATGAAAGCCGCTAGAGGAGCCGATCATGTTTTCTGGGAAACAACTATGGAGACGTCGGTAGGGGACTATAGATACATCGACGGCATTAACATAGCACACGGTGGGAAGACATCTGCTTTGTTATATAGGTATGGACATGCTTATAATCAAAAGCGAAGGATTGAGGAGACGTGGGAGATTGATGAGGTTGATTTCAATCTTAATGGGTTGTCCATGGAGTGCTTTCTGCCTCCTTCCGACCTCAAGAGGGATGATGGAGATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

378

Amino Acids

41.9

Weight (kDa)

5.72

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 125 - 367 9.6e-114 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015873)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75160
fragaria_vesca FvH4_2g39940
malus_domestica MD08G1034000.v1.1 MD15G1030800.v1.1
prunus_persica Prupe.1G381000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0300821
rosa_laevigata RLG00000011297
rosa_multiflora Rmu_co8409361.1_g000001 Rmu_sc0008947.1_g000004
rosa_roxburghii Rroxscaffold_7G00167380
rosa_rugosa Rorug06G0299300
rosa_samantha Rh6AG412400 Rh6BG419600 Rh6CG427100 Rh6DG412400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1011
AarI CACCTGC 1 cut(s) 379
AasI GACNNNNNNGTC 1 cut(s) 627
AatII GACGTC 1 cut(s) 923
Acc36I ACCTGC 1 cut(s) 379
AccB1I GGYRCC 2 cut(s) 58, 560
AccII CGCG 2 cut(s) 268, 305
AciI CCGC 7 cut(s) 99, 102, 109, 303, 342, 350, 876
AclWI GGATC 3 cut(s) 412, 644, 657
AcuI CTGAAG 2 cut(s) 224, 799
AcyI GRCGYC 2 cut(s) 561, 920
AdeI CACNNNGTG 1 cut(s) 968
AfaI GTAC 4 cut(s) 311, 318, 512, 812
AfiI CCNNNNNNNGG 7 cut(s) 108, 241, 507, 570, 807, 847, 1114
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 4 cut(s) 358, 740, 761, 1063
AjiI CACGTC 1 cut(s) 1038
AjuI GAANNNNNNNTTGG 2 cut(s) 659, 691
AluBI AGCT 4 cut(s) 214, 335, 736, 859
AluI AGCT 4 cut(s) 214, 335, 736, 859
Alw21I GWGCWC 1 cut(s) 662
Alw26I GTCTC 2 cut(s) 911, 1028
AlwI GGATC 3 cut(s) 412, 644, 657
Ama87I CYCGRG 1 cut(s) 53
AoxI GGCC 3 cut(s) 37, 259, 610
ApeKI GCWGC 2 cut(s) 93, 332
ArsI GACNNNNNNTTYG 2 cut(s) 967, 999
AspLEI GCGC 3 cut(s) 270, 548, 563
AspS9I GGNCC 1 cut(s) 611
AsuHPI GGTGA 2 cut(s) 403, 874
AvaI CYCGRG 1 cut(s) 53
BaeGI GKGCMC 1 cut(s) 17
BamHI GGATCC 1 cut(s) 649
BanI GGYRCC 2 cut(s) 58, 560
BbsI GAAGAC 1 cut(s) 980
Bbv12I GWGCWC 1 cut(s) 662
BbvI GCAGC 2 cut(s) 105, 344
BccI CCATC 8 cut(s) 29, 166, 281, 296, 391, 551, 713, 1115
BceAI ACGGC 4 cut(s) 347, 548, 678, 964
BcoDI GTCTC 2 cut(s) 911, 1028
BfaI CTAG 2 cut(s) 347, 879
BfmI CTRYAG 1 cut(s) 934
BfoI RGCGCY 2 cut(s) 549, 564
BfrI CTTAAG 1 cut(s) 209
BfuAI ACCTGC 1 cut(s) 379
BisI GCNGC 5 cut(s) 94, 102, 333, 351, 876
BlsI GCNGC 5 cut(s) 95, 103, 334, 352, 877
BmeT110I CYCGRG 1 cut(s) 53
BmgBI CACGTC 1 cut(s) 1038
BmgT120I GGNCC 1 cut(s) 611
BmiI GGNNCC 4 cut(s) 60, 562, 651, 886
BmsI GCATC 1 cut(s) 670
BpiI GAAGAC 1 cut(s) 980
BpuEI CTTGAG 2 cut(s) 716, 1094
BsaBI GATNNNNATC 2 cut(s) 1062, 1125
BsaHI GRCGYC 2 cut(s) 561, 920
BsaJI CCNNGG 2 cut(s) 653, 1080
BsaWI WCCGGW 2 cut(s) 581, 692
Bsc4I CCNNNNNNNGG 7 cut(s) 108, 241, 507, 570, 807, 847, 1114
Bse1I ACTGG 2 cut(s) 525, 841
Bse3DI GCAATG 3 cut(s) 104, 203, 247
Bse8I GATNNNNATC 2 cut(s) 1062, 1125
BseDI CCNNGG 2 cut(s) 653, 1080
BseGI GGATG 5 cut(s) 158, 402, 685, 773, 1123
BseJI GATNNNNATC 2 cut(s) 1062, 1125
BseLI CCNNNNNNNGG 7 cut(s) 108, 241, 507, 570, 807, 847, 1114
BseMI GCAATG 3 cut(s) 104, 203, 247
BseNI ACTGG 2 cut(s) 525, 841
BseRI GAGGAG 4 cut(s) 216, 670, 897, 1046
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 2 cut(s) 105, 344
BseYI CCCAGC 1 cut(s) 745
BsgI GTGCAG 1 cut(s) 399
Bsh1236I CGCG 2 cut(s) 268, 305
BshFI GGCC 3 cut(s) 39, 261, 612
BshNI GGYRCC 2 cut(s) 58, 560
BsiHKAI GWGCWC 1 cut(s) 662
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 5 cut(s) 176, 531, 549, 582, 693
BslFI GGGAC 1 cut(s) 944
BslI CCNNNNNNNGG 7 cut(s) 108, 241, 507, 570, 807, 847, 1114
BsmAI GTCTC 2 cut(s) 911, 1028
BsmBI CGTCTC 2 cut(s) 911, 1028
BsmFI GGGAC 1 cut(s) 944
BsmI GAATGC 1 cut(s) 128
BsnI GGCC 3 cut(s) 39, 261, 612
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 2 cut(s) 17, 662
Bsp1407I TGTACA 1 cut(s) 316
Bsp143I GATC 4 cut(s) 404, 649, 889, 1126
Bsp19I CCATGG 1 cut(s) 1080
BspACI CCGC 7 cut(s) 99, 102, 109, 303, 342, 350, 876
BspANI GGCC 3 cut(s) 39, 261, 612
BspFNI CGCG 2 cut(s) 268, 305
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 4 cut(s) 60, 562, 651, 886
BspMI ACCTGC 1 cut(s) 379
BspPI GGATC 3 cut(s) 412, 644, 657
BspT107I GGYRCC 2 cut(s) 58, 560
BspTI CTTAAG 1 cut(s) 209
BsrDI GCAATG 3 cut(s) 104, 203, 247
BsrGI TGTACA 1 cut(s) 316
BsrI ACTGG 2 cut(s) 525, 841
BssECI CCNNGG 2 cut(s) 653, 1080
BssMI GATC 4 cut(s) 404, 649, 889, 1126
BssNI GRCGYC 2 cut(s) 561, 920
BssT1I CCWWGG 1 cut(s) 1080
Bst4CI ACNGT 3 cut(s) 49, 464, 968
Bst6I CTCTTC 1 cut(s) 243
BstACI GRCGYC 2 cut(s) 561, 920
BstAFI CTTAAG 1 cut(s) 209
BstAPI GCANNNNNTGC 1 cut(s) 121
BstAUI TGTACA 1 cut(s) 316
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 1 cut(s) 514
BstDSI CCRYGG 1 cut(s) 1080
BstF5I GGATG 5 cut(s) 158, 402, 685, 773, 1123
BstFNI CGCG 2 cut(s) 268, 305
BstH2I RGCGCY 2 cut(s) 549, 564
BstHHI GCGC 3 cut(s) 270, 548, 563
BstKTI GATC 4 cut(s) 407, 652, 892, 1129
BstMAI GTCTC 2 cut(s) 911, 1028
BstMBI GATC 4 cut(s) 404, 649, 889, 1126
BstMWI GCNNNNNNNGC 6 cut(s) 121, 258, 267, 302, 359, 884
BstNSI RCATGY 1 cut(s) 1007
BstSFI CTRYAG 1 cut(s) 934
BstSLI GKGCMC 1 cut(s) 17
BstUI CGCG 2 cut(s) 268, 305
BstV1I GCAGC 2 cut(s) 105, 344
BstV2I GAAGAC 1 cut(s) 980
BstX2I RGATCY 2 cut(s) 404, 649
BstYI RGATCY 2 cut(s) 404, 649
BsuRI GGCC 3 cut(s) 39, 261, 612
BtgI CCRYGG 1 cut(s) 1080
BtgZI GCGATG 1 cut(s) 438
BtrI CACGTC 1 cut(s) 1038
BtsCI GGATG 5 cut(s) 158, 402, 685, 773, 1123
BtsIMutI CAGTG 1 cut(s) 469
BveI ACCTGC 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 126
CciI TCATGA 1 cut(s) 132
CfoI GCGC 3 cut(s) 270, 548, 563
Cfr13I GGNCC 1 cut(s) 611
Csp6I GTAC 4 cut(s) 310, 317, 511, 811
CviAII CATG 6 cut(s) 133, 570, 599, 893, 1004, 1081
CviQI GTAC 4 cut(s) 310, 317, 511, 811
DdeI CTNAG 1 cut(s) 514
DinI GGCGCC 1 cut(s) 562
DpnI GATC 4 cut(s) 406, 651, 891, 1128
DpnII GATC 4 cut(s) 404, 649, 889, 1126
DraIII CACNNNGTG 1 cut(s) 968
DrdI GACNNNNNNGTC 1 cut(s) 627
DseDI GACNNNNNNGTC 1 cut(s) 627
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
EciI GGCGGA 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 1080
Eco57I CTGAAG 2 cut(s) 224, 799
Eco88I CYCGRG 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 611
EcoT14I CCWWGG 1 cut(s) 1080
EgeI GGCGCC 1 cut(s) 562
EheI GGCGCC 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 1080
Esp3I CGTCTC 2 cut(s) 911, 1028
FaeI CATG 6 cut(s) 136, 573, 602, 896, 1007, 1084
FalI AAGNNNNNCTT 2 cut(s) 24, 56
FaqI GGGAC 1 cut(s) 944
FatI CATG 6 cut(s) 132, 569, 598, 892, 1003, 1080
FauNDI CATATG 1 cut(s) 803
Fnu4HI GCNGC 5 cut(s) 94, 102, 333, 351, 876
FokI GGATG 5 cut(s) 145, 409, 692, 760, 1130
Fsp4HI GCNGC 5 cut(s) 94, 102, 333, 351, 876
FspBI CTAG 2 cut(s) 347, 879
GlaI GCGC 3 cut(s) 269, 547, 562
GluI GCNGC 5 cut(s) 94, 102, 333, 351, 876
GsaI CCCAGC 1 cut(s) 749
HaeII RGCGCY 2 cut(s) 549, 564
HaeIII GGCC 3 cut(s) 39, 261, 612
HapII CCGG 5 cut(s) 176, 531, 549, 582, 693
HhaI GCGC 3 cut(s) 270, 548, 563
Hin1I GRCGYC 2 cut(s) 561, 920
Hin1II CATG 6 cut(s) 136, 573, 602, 896, 1007, 1084
Hin6I GCGC 3 cut(s) 268, 546, 561
HinP1I GCGC 3 cut(s) 268, 546, 561
HindIII AAGCTT 2 cut(s) 212, 734
HinfI GANTC 2 cut(s) 496, 574
HpaII CCGG 5 cut(s) 176, 531, 549, 582, 693
HphI GGTGA 2 cut(s) 403, 874
Hpy166II GTNNAC 2 cut(s) 460, 793
Hpy188I TCNGA 4 cut(s) 278, 409, 654, 1105
Hpy188III TCNNGA 4 cut(s) 53, 133, 500, 1111
Hpy8I GTNNAC 2 cut(s) 460, 793
Hpy99I CGWCG 7 cut(s) 448, 451, 454, 569, 633, 925, 950
HpyAV CCTTC 4 cut(s) 603, 635, 1017, 1110
HpyCH4III ACNGT 3 cut(s) 49, 464, 968
HpyCH4IV ACGT 2 cut(s) 920, 1037
HpyCH4V TGCA 5 cut(s) 128, 380, 415, 683, 690
HpyF10VI GCNNNNNNNGC 6 cut(s) 121, 258, 267, 302, 359, 884
HpyF3I CTNAG 1 cut(s) 514
HpySE526I ACGT 2 cut(s) 920, 1037
Hsp92I GRCGYC 2 cut(s) 561, 920
Hsp92II CATG 6 cut(s) 136, 573, 602, 896, 1007, 1084
HspAI GCGC 3 cut(s) 268, 546, 561
KasI GGCGCC 1 cut(s) 560
Kzo9I GATC 4 cut(s) 404, 649, 889, 1126
LmnI GCTCC 2 cut(s) 657, 884
Lsp1109I GCAGC 2 cut(s) 105, 344
LweI GCATC 1 cut(s) 670
MaeI CTAG 2 cut(s) 347, 879
MaeII ACGT 2 cut(s) 920, 1037
MalI GATC 4 cut(s) 406, 651, 891, 1128
MboI GATC 4 cut(s) 404, 649, 889, 1126
MboII GAAGA 7 cut(s) 44, 83, 230, 728, 749, 877, 985
MflI RGATCY 2 cut(s) 404, 649
MhlI GDGCHC 2 cut(s) 17, 662
Mly113I GGCGCC 1 cut(s) 561
MmeI TCCRAC 1 cut(s) 1128
MseI TTAA 4 cut(s) 150, 210, 954, 1068
MslI CAYNNNNRTG 1 cut(s) 109
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 5 cut(s) 176, 531, 549, 582, 693
Mva1269I GAATGC 1 cut(s) 128
MvnI CGCG 2 cut(s) 268, 305
MwoI GCNNNNNNNGC 6 cut(s) 121, 258, 267, 302, 359, 884
NarI GGCGCC 1 cut(s) 561
NcoI CCATGG 1 cut(s) 1080
NdeI CATATG 1 cut(s) 803
NdeII GATC 4 cut(s) 404, 649, 889, 1126
NlaIII CATG 6 cut(s) 136, 573, 602, 896, 1007, 1084
NlaIV GGNNCC 4 cut(s) 60, 562, 651, 886
NspI RCATGY 1 cut(s) 1007
PaeR7I CTCGAG 1 cut(s) 53
PagI TCATGA 1 cut(s) 132
PaqCI CACCTGC 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 449
PctI GAATGC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 496, 574
PkrI GCNGC 5 cut(s) 95, 103, 334, 352, 877
PluTI GGCGCC 1 cut(s) 564
PsiI TTATAA 1 cut(s) 1011
PspFI CCCAGC 1 cut(s) 745
PspN4I GGNNCC 4 cut(s) 60, 562, 651, 886
PspPI GGNCC 1 cut(s) 611
PsuI RGATCY 2 cut(s) 404, 649
RsaI GTAC 4 cut(s) 311, 318, 512, 812
RsaNI GTAC 4 cut(s) 310, 317, 511, 811
RseI CAYNNNNRTG 1 cut(s) 109
SaqAI TTAA 4 cut(s) 150, 210, 954, 1068
SatI GCNGC 5 cut(s) 94, 102, 333, 351, 876
Sau3AI GATC 4 cut(s) 404, 649, 889, 1126
Sau96I GGNCC 1 cut(s) 611
SduI GDGCHC 2 cut(s) 17, 662
SfaNI GCATC 1 cut(s) 670
SfcI CTRYAG 1 cut(s) 934
SfoI GGCGCC 1 cut(s) 562
Sfr274I CTCGAG 1 cut(s) 53
SlaI CTCGAG 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 109
SmlI CTYRAG 4 cut(s) 53, 209, 731, 1109
SmoI CTYRAG 4 cut(s) 53, 209, 731, 1109
SsiI CCGC 7 cut(s) 99, 102, 109, 303, 342, 350, 876
SspDI GGCGCC 1 cut(s) 560
SspMI CTAG 2 cut(s) 347, 879
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 3 cut(s) 49, 464, 968
TaiI ACGT 2 cut(s) 923, 1040
TaqI TCGA 4 cut(s) 54, 291, 443, 945
TatI WGTACW 2 cut(s) 309, 316
TauI GCSGC 3 cut(s) 104, 353, 878
TfiI GAWTC 2 cut(s) 496, 574
Tru1I TTAA 4 cut(s) 150, 210, 954, 1068
Tru9I TTAA 4 cut(s) 150, 210, 954, 1068
TscAI CASTG 1 cut(s) 469
TseI GCWGC 2 cut(s) 93, 332
TspDTI ATGAA 6 cut(s) 45, 121, 149, 236, 715, 884
TspRI CASTG 1 cut(s) 469
Vha464I CTTAAG 1 cut(s) 209
XceI RCATGY 1 cut(s) 1007
XhoI CTCGAG 1 cut(s) 53
XspI CTAG 2 cut(s) 347, 879
ZraI GACGTC 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.