MD15G1030800.v1.1

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015873
Physical Location & Seq
Forward (+)
1891050 .. 1892336
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1030800.v1.1.491

Sequence Viewer

Length: 918 bp
ATGCGGAAGCTATGCCCTAACCTAGACAAAGAAGATGGCCTGGATACCGTCCTCGAGGTGCCTATTCCGGAGGAAATGTTCACCAGCATGGGCAGCAACGTCGTGTTGCGGTTGGCGAATCTCTGTTCTTTCATGAAAGATCAGTCCCACGTCGAGAAAAATTCAAGATCATCTCATCCCTCGTCGGGATCAAATAATGAGTTCATGGCATTGCTCAAGCTTGTTGGTTCTCCCCTCATCCCCCTTCAGGTTGATTGCGACAGTGCACTCTCCCGCCCTATAAAAGATTGTTCCATTGAGGGTTCTACGGCGAAATATATAGTGCAACAATATGGGGCGGCAACCGGGGGACTGGCGGCGTTGAAGTCGGTGAATAGCATGTACGCCGTTGGGGAGGTTAAGATGGTGGGATCCGAAATGCGCCAAGGTGATGATAGTGTACACGTTCAGAGCAAAGTCAATAGCGAGGTTGGTGGGTTTGTCCTATGGCAAAAGAATCCCGACTTGTGGTACTTGGAATTGGTTGTTTCCGGCTTGAAAGTCAGTGCTGGAAGTGATGGCAAGGTCGTGTGGAATCAATCTGGCTCACAACCTTGCCGTGCGAATAAAGGCCCTCCAAGACCCCTCCGTCGTTTCTTTCAGGGTTTGGGCCCCAGGAGCACTGCCAACTTGTTCCTAGATGCAATGTGCACAGGAGAGAAAAACATCAACAAAGAAGAATGCTTCATCCTCAAGCTCAAAACCCCCACGGAAATACTCCAATCCCAAAGCAGCCCCCAGACTGAAATAGTCCATCACACCATCTGGGGCTACTTCAGCCAGCGGACGGGACTGCTAATCCAGTTCGAGGACACCAAGCTGGTCAAGATGAGGTCGCGAAACGACGACGTTTCTGGGAGACGACGATGGAGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

306

Amino Acids

33.84

Weight (kDa)

8.91

Isoelectric Point (pI)

60.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 127 - 295 1.7e-75 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015873)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75160
fragaria_vesca FvH4_2g39940
malus_domestica MD08G1034000.v1.1 MD15G1030800.v1.1
prunus_persica Prupe.1G381000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0300821
rosa_laevigata RLG00000011297
rosa_multiflora Rmu_co8409361.1_g000001 Rmu_sc0008947.1_g000004
rosa_roxburghii Rroxscaffold_7G00167380
rosa_rugosa Rorug06G0299300
rosa_samantha Rh6AG412400 Rh6BG419600 Rh6CG427100 Rh6DG412400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 627
AbsI CCTCGAGG 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 58
AccII CGCG 1 cut(s) 877
AccIII TCCGGA 1 cut(s) 67
AciI CCGC 6 cut(s) 4, 109, 274, 338, 356, 823
AclWI GGATC 3 cut(s) 196, 405, 418
AcsI RAATTY 1 cut(s) 160
AcuI CTGAAG 2 cut(s) 230, 799
AfaI GTAC 3 cut(s) 383, 441, 512
AfiI CCNNNNNNNGG 5 cut(s) 185, 247, 507, 826, 847
AflIII ACRYGT 1 cut(s) 442
AgsI TTSAA 3 cut(s) 165, 364, 538
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 2 cut(s) 39, 653
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
AluBI AGCT 4 cut(s) 10, 220, 736, 859
AluI AGCT 4 cut(s) 10, 220, 736, 859
Alw21I GWGCWC 3 cut(s) 268, 662, 692
Alw26I GTCTC 2 cut(s) 892, 904
Alw44I GTGCAC 2 cut(s) 264, 688
AlwI GGATC 3 cut(s) 196, 405, 418
Ama87I CYCGRG 1 cut(s) 53
Aor13HI TCCGGA 1 cut(s) 67
AoxI GGCC 3 cut(s) 37, 610, 649
ApaI GGGCCC 1 cut(s) 653
ApaLI GTGCAC 2 cut(s) 264, 688
ApeKI GCWGC 2 cut(s) 93, 771
ApoI RAATTY 1 cut(s) 160
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 3 cut(s) 611, 649, 650
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 3 cut(s) 73, 382, 440
AvaI CYCGRG 1 cut(s) 53
BaeGI GKGCMC 3 cut(s) 268, 653, 692
BamHI GGATCC 1 cut(s) 410
BanI GGYRCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 653
Bbv12I GWGCWC 3 cut(s) 268, 662, 692
BbvI GCAGC 2 cut(s) 105, 783
BccI CCATC 6 cut(s) 29, 397, 551, 801, 809, 900
BceAI ACGGC 3 cut(s) 324, 371, 582
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BciT130I CCWGG 2 cut(s) 41, 655
BciVI GTATCC 1 cut(s) 37
BcnI CCSGG 1 cut(s) 346
BcoDI GTCTC 2 cut(s) 892, 904
BfaI CTAG 2 cut(s) 23, 677
BfuI GTATCC 1 cut(s) 37
BisI GCNGC 4 cut(s) 94, 339, 357, 772
BlsI GCNGC 4 cut(s) 95, 340, 358, 773
Bme1390I CCNGG 3 cut(s) 41, 346, 655
BmeT110I CYCGRG 1 cut(s) 53
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 3 cut(s) 611, 649, 650
BmiI GGNNCC 4 cut(s) 60, 412, 651, 652
BmrFI CCNGG 3 cut(s) 41, 346, 655
BmsI GCATC 1 cut(s) 670
BpuEI CTTGAG 2 cut(s) 200, 716
BpuMI CCSGG 1 cut(s) 346
BsaJI CCNNGG 4 cut(s) 345, 424, 653, 747
BsaWI WCCGGW 1 cut(s) 67
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 247, 507, 826, 847
Bse1I ACTGG 2 cut(s) 357, 841
Bse3DI GCAATG 2 cut(s) 209, 690
BseAI TCCGGA 1 cut(s) 67
BseBI CCWGG 2 cut(s) 41, 655
BseDI CCNNGG 4 cut(s) 345, 424, 653, 747
BseGI GGATG 3 cut(s) 175, 237, 726
BseLI CCNNNNNNNGG 5 cut(s) 185, 247, 507, 826, 847
BseMI GCAATG 2 cut(s) 209, 690
BseNI ACTGG 2 cut(s) 357, 841
BseSI GKGCMC 3 cut(s) 268, 653, 692
BseXI GCAGC 2 cut(s) 105, 783
Bsh1236I CGCG 1 cut(s) 877
BshFI GGCC 3 cut(s) 39, 612, 651
BshNI GGYRCC 1 cut(s) 58
BsiHKAI GWGCWC 3 cut(s) 268, 662, 692
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 3 cut(s) 68, 345, 531
BslFI GGGAC 3 cut(s) 130, 363, 843
BslI CCNNNNNNNGG 5 cut(s) 185, 247, 507, 826, 847
BsmAI GTCTC 2 cut(s) 892, 904
BsmBI CGTCTC 2 cut(s) 892, 904
BsmFI GGGAC 3 cut(s) 130, 363, 843
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 3 cut(s) 39, 612, 651
BsoBI CYCGRG 1 cut(s) 53
Bsp120I GGGCCC 1 cut(s) 649
Bsp1286I GDGCHC 4 cut(s) 268, 653, 662, 692
Bsp13I TCCGGA 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 439
Bsp143I GATC 4 cut(s) 139, 167, 188, 410
Bsp68I TCGCGA 1 cut(s) 877
BspACI CCGC 6 cut(s) 4, 109, 274, 338, 356, 823
BspANI GGCC 3 cut(s) 39, 612, 651
BspEI TCCGGA 1 cut(s) 67
BspFNI CGCG 1 cut(s) 877
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 4 cut(s) 60, 412, 651, 652
BspPI GGATC 3 cut(s) 196, 405, 418
BspT107I GGYRCC 1 cut(s) 58
BsrDI GCAATG 2 cut(s) 209, 690
BsrGI TGTACA 1 cut(s) 439
BsrI ACTGG 2 cut(s) 357, 841
BssECI CCNNGG 4 cut(s) 345, 424, 653, 747
BssMI GATC 4 cut(s) 139, 167, 188, 410
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 2 cut(s) 41, 655
Bst4CI ACNGT 3 cut(s) 49, 263, 915
BstAUI TGTACA 1 cut(s) 439
BstC8I GCNNGC 1 cut(s) 821
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 3 cut(s) 175, 237, 726
BstFNI CGCG 1 cut(s) 877
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 4 cut(s) 142, 170, 191, 413
BstMAI GTCTC 2 cut(s) 892, 904
BstMBI GATC 4 cut(s) 139, 167, 188, 410
BstMWI GCNNNNNNNGC 3 cut(s) 93, 657, 816
BstNI CCWGG 2 cut(s) 41, 655
BstNSI RCATGY 1 cut(s) 382
BstSCI CCNGG 3 cut(s) 39, 344, 653
BstSLI GKGCMC 3 cut(s) 268, 653, 692
BstUI CGCG 1 cut(s) 877
BstV1I GCAGC 2 cut(s) 105, 783
BstX2I RGATCY 1 cut(s) 410
BstYI RGATCY 1 cut(s) 410
BsuI GTATCC 1 cut(s) 37
BsuRI GGCC 3 cut(s) 39, 612, 651
BtgI CCRYGG 1 cut(s) 747
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 3 cut(s) 175, 237, 726
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 3 cut(s) 268, 550, 660
BtuMI TCGCGA 1 cut(s) 877
Cac8I GCNNGC 1 cut(s) 821
CciI TCATGA 1 cut(s) 132
CfoI GCGC 1 cut(s) 423
Cfr13I GGNCC 3 cut(s) 611, 649, 650
Csp6I GTAC 3 cut(s) 382, 440, 511
CviAII CATG 4 cut(s) 88, 133, 205, 379
CviQI GTAC 3 cut(s) 382, 440, 511
DpnI GATC 4 cut(s) 141, 169, 190, 412
DpnII GATC 4 cut(s) 139, 167, 188, 410
DrdI GACNNNNNNGTC 1 cut(s) 627
DseDI GACNNNNNNGTC 1 cut(s) 627
Eco130I CCWWGG 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 653
Eco57I CTGAAG 2 cut(s) 230, 799
Eco88I CYCGRG 1 cut(s) 53
EcoO109I RGGNCCY 2 cut(s) 611, 650
EcoRII CCWGG 2 cut(s) 39, 653
EcoT14I CCWWGG 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 424
Esp3I CGTCTC 2 cut(s) 892, 904
FaeI CATG 4 cut(s) 91, 136, 208, 382
FaqI GGGAC 3 cut(s) 130, 363, 843
FatI CATG 4 cut(s) 87, 132, 204, 378
FauI CCCGC 1 cut(s) 281
Fnu4HI GCNGC 4 cut(s) 94, 339, 357, 772
FokI GGATG 3 cut(s) 162, 224, 713
FriOI GRGCYC 1 cut(s) 653
Fsp4HI GCNGC 4 cut(s) 94, 339, 357, 772
FspBI CTAG 2 cut(s) 23, 677
GlaI GCGC 1 cut(s) 422
GluI GCNGC 4 cut(s) 94, 339, 357, 772
HaeIII GGCC 3 cut(s) 39, 612, 651
HapII CCGG 3 cut(s) 68, 345, 531
HhaI GCGC 1 cut(s) 423
Hin1II CATG 4 cut(s) 91, 136, 208, 382
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 3 cut(s) 118, 496, 574
HpaII CCGG 3 cut(s) 68, 345, 531
HphI GGTGA 3 cut(s) 73, 382, 440
Hpy166II GTNNAC 5 cut(s) 81, 266, 440, 442, 690
Hpy188I TCNGA 2 cut(s) 415, 450
Hpy188III TCNNGA 8 cut(s) 68, 133, 154, 165, 186, 500, 865, 876
Hpy8I GTNNAC 5 cut(s) 81, 266, 440, 442, 690
Hpy99I CGWCG 7 cut(s) 104, 155, 187, 633, 887, 890, 906
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 3 cut(s) 49, 263, 915
HpyCH4IV ACGT 4 cut(s) 99, 150, 444, 888
HpyCH4V TGCA 4 cut(s) 266, 325, 683, 690
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 657, 816
HpySE526I ACGT 4 cut(s) 99, 150, 444, 888
Hsp92II CATG 4 cut(s) 91, 136, 208, 382
HspAI GCGC 1 cut(s) 421
Kpn2I TCCGGA 1 cut(s) 67
Kzo9I GATC 4 cut(s) 139, 167, 188, 410
LmnI GCTCC 1 cut(s) 657
Lsp1109I GCAGC 2 cut(s) 105, 783
LweI GCATC 1 cut(s) 670
MaeI CTAG 2 cut(s) 23, 677
MaeII ACGT 4 cut(s) 99, 150, 444, 888
MalI GATC 4 cut(s) 141, 169, 190, 412
MboI GATC 4 cut(s) 139, 167, 188, 410
MboII GAAGA 2 cut(s) 44, 728
MflI RGATCY 1 cut(s) 410
MhlI GDGCHC 4 cut(s) 268, 653, 662, 692
MluCI AATT 2 cut(s) 160, 518
MroI TCCGGA 1 cut(s) 67
MseI TTAA 1 cut(s) 399
MslI CAYNNNNRTG 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 823
MspI CCGG 3 cut(s) 68, 345, 531
MspR9I CCNGG 3 cut(s) 41, 346, 655
Mva1269I GAATGC 1 cut(s) 725
MvaI CCWGG 2 cut(s) 41, 655
MvnI CGCG 1 cut(s) 877
MwoI GCNNNNNNNGC 3 cut(s) 93, 657, 816
NciI CCSGG 1 cut(s) 346
NdeII GATC 4 cut(s) 139, 167, 188, 410
NlaIII CATG 4 cut(s) 91, 136, 208, 382
NlaIV GGNNCC 4 cut(s) 60, 412, 651, 652
NruI TCGCGA 1 cut(s) 877
NspI RCATGY 1 cut(s) 382
PaeR7I CTCGAG 1 cut(s) 53
PagI TCATGA 1 cut(s) 132
PctI GAATGC 1 cut(s) 725
PfeI GAWTC 3 cut(s) 118, 496, 574
PkrI GCNGC 4 cut(s) 95, 340, 358, 773
Psp6I CCWGG 2 cut(s) 39, 653
PspGI CCWGG 2 cut(s) 39, 653
PspN4I GGNNCC 4 cut(s) 60, 412, 651, 652
PspOMI GGGCCC 1 cut(s) 649
PspPI GGNCC 3 cut(s) 611, 649, 650
PspXI VCTCGAGB 1 cut(s) 53
PsuI RGATCY 1 cut(s) 410
RruI TCGCGA 1 cut(s) 877
RsaI GTAC 3 cut(s) 383, 441, 512
RsaNI GTAC 3 cut(s) 382, 440, 511
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 4 cut(s) 94, 339, 357, 772
Sau3AI GATC 4 cut(s) 139, 167, 188, 410
Sau96I GGNCC 3 cut(s) 611, 649, 650
ScrFI CCNGG 3 cut(s) 41, 346, 655
SduI GDGCHC 4 cut(s) 268, 653, 662, 692
SfaNI GCATC 1 cut(s) 670
Sfr274I CTCGAG 1 cut(s) 53
SlaI CTCGAG 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 3 cut(s) 53, 215, 731
SmoI CTYRAG 3 cut(s) 53, 215, 731
Sse9I AATT 2 cut(s) 160, 518
SsiI CCGC 6 cut(s) 4, 109, 274, 338, 356, 823
SspMI CTAG 2 cut(s) 23, 677
StyD4I CCNGG 3 cut(s) 39, 344, 653
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 3 cut(s) 49, 263, 915
TaiI ACGT 4 cut(s) 102, 153, 447, 891
TaqI TCGA 3 cut(s) 54, 153, 846
TasI AATT 2 cut(s) 160, 518
TatI WGTACW 1 cut(s) 439
TauI GCSGC 2 cut(s) 341, 359
TfiI GAWTC 3 cut(s) 118, 496, 574
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 3 cut(s) 268, 550, 667
TseI GCWGC 2 cut(s) 93, 771
TspDTI ATGAA 4 cut(s) 121, 149, 193, 715
TspGWI ACGGA 2 cut(s) 617, 764
TspRI CASTG 3 cut(s) 268, 550, 667
VneI GTGCAC 2 cut(s) 264, 688
XapI RAATTY 1 cut(s) 160
XceI RCATGY 1 cut(s) 382
XhoI CTCGAG 1 cut(s) 53
XspI CTAG 2 cut(s) 23, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.