Rh6AG412400

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015873
Physical Location & Seq
Forward (+)
60876830 .. 60878877
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG412400.1

Sequence Viewer

Length: 849 bp
ATGATGCAGGAGGCTTCTACTGCGAAATACATTGTACAACAATATGTAGCAGCTATCGGGGGACTAGCGGCATTGAATGCCGTGAATAGTATGTGTGCAGTAGGGCAGGTGAAGATGGTGGGATCTGAAATGCACAAAGCCGATGATAACAGTGTTCAAACGACGACGAAAGTCAACAGTGAAGTCGGTGGATTTGTTCTTTGGCAAAAGAATCCTGACTTGTGGTACTTAGAGCTGGTTGTTTCCGGCTACAAGGTAAGCGCCGGAAGTGATGGCTCTGTTGCATGGAATCAGTCCGGTTCTCAACCTTCTCATGCCAATAAAGGCCCCCCAAGACCCCTCCGTCGCTTCTTTCAGGGATTGGATCCGAGGAGCACAGCCAACTTGTTTTTGGACGCAGTATGCACCGGAGAGAAAACCATCAACAAAGAAGAATGTTTCATACTCAAGCTTGAAACGCCAGCAGATATTCTTCAATCACAAAGCACATCCCAAACTGAAATAGTCCACCACACGATATGGGGGTACTTCAGCCAAAGAACAGGGCTTCTTGTCCAGTTTGAGGACACAAAATTGGTGAAAATGAAGGCCGCTAGAGGAGACGACCACGTTTTTTGGGAAACAACGATGGAGACGTTGGTAGGGGACTATAGATACGTTGATGGCATTAACATAGCACATGGTGGTAAGACATCTGCATTGTTATTTAGGTATGGACATGCTTATAATCAAAAGCGAAGGATTGAGGAGACGTGGGAGATTGATGAGGTTGATTTCAATATTTGTGGGTTGTCCATGGATTGTTTTCTGCCTCCTTCCGACCTCAAGAGGGATGATGCAGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

282

Amino Acids

31.41

Weight (kDa)

5.4

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 31 - 272 8.1e-117 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015873)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75160
fragaria_vesca FvH4_2g39940
malus_domestica MD08G1034000.v1.1 MD15G1030800.v1.1
prunus_persica Prupe.1G381000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0300821
rosa_laevigata RLG00000011297
rosa_multiflora Rmu_co8409361.1_g000001 Rmu_sc0008947.1_g000004
rosa_roxburghii Rroxscaffold_7G00167380
rosa_rugosa Rorug06G0299300
rosa_samantha Rh6AG412400 Rh6BG419600 Rh6CG427100 Rh6DG412400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AarI CACCTGC 1 cut(s) 97
AasI GACNNNNNNGTC 1 cut(s) 342
Acc36I ACCTGC 1 cut(s) 97
AciI CCGC 2 cut(s) 68, 591
AclWI GGATC 3 cut(s) 130, 359, 372
AcuI CTGAAG 1 cut(s) 514
AdeI CACNNNGTG 1 cut(s) 683
AfaI GTAC 3 cut(s) 36, 227, 527
AfiI CCNNNNNNNGG 2 cut(s) 562, 829
AgsI TTSAA 5 cut(s) 76, 158, 455, 476, 778
AjiI CACGTC 1 cut(s) 753
AluBI AGCT 3 cut(s) 53, 235, 451
AluI AGCT 3 cut(s) 53, 235, 451
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 3 cut(s) 594, 626, 743
AlwI GGATC 3 cut(s) 130, 359, 372
AoxI GGCC 2 cut(s) 325, 588
ApeKI GCWGC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 121, 589
BamHI GGATCC 1 cut(s) 364
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 1 cut(s) 62
BccI CCATC 5 cut(s) 109, 266, 428, 622, 656
BceAI ACGGC 1 cut(s) 65
BcoDI GTCTC 3 cut(s) 594, 626, 743
BfaI CTAG 2 cut(s) 65, 594
BfmI CTRYAG 1 cut(s) 649
BfoI RGCGCY 1 cut(s) 264
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 3 cut(s) 51, 69, 591
BlsI GCNGC 3 cut(s) 52, 70, 592
BmgBI CACGTC 1 cut(s) 753
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 2 cut(s) 328, 366
BmsI GCATC 1 cut(s) 826
BoxI GACNNNNGTC 1 cut(s) 170
BpuEI CTTGAG 2 cut(s) 431, 809
BsaJI CCNNGG 2 cut(s) 368, 795
BsaWI WCCGGW 2 cut(s) 296, 407
Bsc4I CCNNNNNNNGG 2 cut(s) 562, 829
Bse1I ACTGG 1 cut(s) 556
BseDI CCNNGG 2 cut(s) 368, 795
BseGI GGATG 2 cut(s) 488, 838
BseLI CCNNNNNNNGG 2 cut(s) 562, 829
BseNI ACTGG 1 cut(s) 556
BseRI GAGGAG 3 cut(s) 385, 612, 761
BseXI GCAGC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 117
BshFI GGCC 2 cut(s) 327, 590
BsiHKAI GWGCWC 1 cut(s) 377
BsiSI CCGG 4 cut(s) 246, 264, 297, 408
BslFI GGGAC 2 cut(s) 75, 659
BslI CCNNNNNNNGG 2 cut(s) 562, 829
BsmAI GTCTC 3 cut(s) 594, 626, 743
BsmBI CGTCTC 3 cut(s) 594, 626, 743
BsmFI GGGAC 2 cut(s) 75, 659
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 2 cut(s) 327, 590
Bsp1286I GDGCHC 1 cut(s) 377
Bsp1407I TGTACA 1 cut(s) 34
Bsp143I GATC 2 cut(s) 122, 364
Bsp19I CCATGG 1 cut(s) 795
BspACI CCGC 2 cut(s) 68, 591
BspANI GGCC 2 cut(s) 327, 590
BspLI GGNNCC 2 cut(s) 328, 366
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 3 cut(s) 130, 359, 372
BsrGI TGTACA 1 cut(s) 34
BsrI ACTGG 1 cut(s) 556
BssECI CCNNGG 2 cut(s) 368, 795
BssMI GATC 2 cut(s) 122, 364
BssT1I CCWWGG 1 cut(s) 795
Bst4CI ACNGT 2 cut(s) 152, 179
BstAPI GCANNNNNTGC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 462
BstDEI CTNAG 1 cut(s) 229
BstDSI CCRYGG 1 cut(s) 795
BstF5I GGATG 2 cut(s) 488, 838
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 2 cut(s) 125, 367
BstMAI GTCTC 3 cut(s) 594, 626, 743
BstMBI GATC 2 cut(s) 122, 364
BstMWI GCNNNNNNNGC 3 cut(s) 20, 77, 457
BstNSI RCATGY 1 cut(s) 722
BstPAI GACNNNNGTC 1 cut(s) 170
BstSFI CTRYAG 1 cut(s) 649
BstV1I GCAGC 1 cut(s) 62
BstX2I RGATCY 2 cut(s) 122, 364
BstYI RGATCY 2 cut(s) 122, 364
BsuRI GGCC 2 cut(s) 327, 590
BtgI CCRYGG 1 cut(s) 795
BtrI CACGTC 1 cut(s) 753
BtsCI GGATG 2 cut(s) 488, 838
BtsIMutI CAGTG 2 cut(s) 157, 184
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 462
CfoI GCGC 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 326
CseI GACGC 1 cut(s) 404
Csp6I GTAC 3 cut(s) 35, 226, 526
CspCI CAANNNNNGTGG 4 cut(s) 596, 631, 766, 801
CviAII CATG 5 cut(s) 285, 314, 680, 719, 796
CviQI GTAC 3 cut(s) 35, 226, 526
DdeI CTNAG 1 cut(s) 229
DpnI GATC 2 cut(s) 124, 366
DpnII GATC 2 cut(s) 122, 364
DraIII CACNNNGTG 1 cut(s) 683
DrdI GACNNNNNNGTC 1 cut(s) 342
DseDI GACNNNNNNGTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 795
Eco57I CTGAAG 1 cut(s) 514
EcoO109I RGGNCCY 1 cut(s) 326
EcoT14I CCWWGG 1 cut(s) 795
ErhI CCWWGG 1 cut(s) 795
Esp3I CGTCTC 3 cut(s) 594, 626, 743
FaeI CATG 5 cut(s) 288, 317, 683, 722, 799
FaqI GGGAC 2 cut(s) 75, 659
FatI CATG 5 cut(s) 284, 313, 679, 718, 795
Fnu4HI GCNGC 3 cut(s) 51, 69, 591
FokI GGATG 2 cut(s) 475, 845
Fsp4HI GCNGC 3 cut(s) 51, 69, 591
FspBI CTAG 2 cut(s) 65, 594
GlaI GCGC 1 cut(s) 262
GluI GCNGC 3 cut(s) 51, 69, 591
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 2 cut(s) 327, 590
HapII CCGG 4 cut(s) 246, 264, 297, 408
HgaI GACGC 1 cut(s) 404
HhaI GCGC 1 cut(s) 263
Hin1II CATG 5 cut(s) 288, 317, 683, 722, 799
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 2 cut(s) 211, 289
HpaII CCGG 4 cut(s) 246, 264, 297, 408
HphI GGTGA 2 cut(s) 121, 589
Hpy166II GTNNAC 2 cut(s) 175, 508
Hpy188I TCNGA 3 cut(s) 127, 369, 820
Hpy188III TCNNGA 2 cut(s) 215, 826
Hpy8I GTNNAC 2 cut(s) 175, 508
Hpy99I CGWCG 3 cut(s) 166, 169, 348
HpyAV CCTTC 4 cut(s) 318, 580, 732, 825
HpyCH4III ACNGT 2 cut(s) 152, 179
HpyCH4IV ACGT 4 cut(s) 609, 635, 657, 752
HpyCH4V TGCA 7 cut(s) 7, 98, 133, 284, 405, 698, 839
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 77, 457
HpyF3I CTNAG 1 cut(s) 229
HpySE526I ACGT 4 cut(s) 609, 635, 657, 752
Hsp92II CATG 5 cut(s) 288, 317, 683, 722, 799
HspAI GCGC 1 cut(s) 261
Kzo9I GATC 2 cut(s) 122, 364
LmnI GCTCC 1 cut(s) 372
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 826
MaeI CTAG 2 cut(s) 65, 594
MaeII ACGT 4 cut(s) 609, 635, 657, 752
MalI GATC 2 cut(s) 124, 366
MboI GATC 2 cut(s) 122, 364
MboII GAAGA 3 cut(s) 124, 443, 464
MflI RGATCY 2 cut(s) 122, 364
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 1 cut(s) 572
MmeI TCCRAC 1 cut(s) 843
MseI TTAA 1 cut(s) 669
MspI CCGG 4 cut(s) 246, 264, 297, 408
Mva1269I GAATGC 1 cut(s) 82
MwoI GCNNNNNNNGC 3 cut(s) 20, 77, 457
NcoI CCATGG 1 cut(s) 795
NdeII GATC 2 cut(s) 122, 364
NlaIII CATG 5 cut(s) 288, 317, 683, 722, 799
NlaIV GGNNCC 2 cut(s) 328, 366
NspI RCATGY 1 cut(s) 722
PaqCI CACCTGC 1 cut(s) 97
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PctI GAATGC 1 cut(s) 82
PfeI GAWTC 2 cut(s) 211, 289
PkrI GCNGC 3 cut(s) 52, 70, 592
PshAI GACNNNNGTC 1 cut(s) 170
PsiI TTATAA 1 cut(s) 726
PspN4I GGNNCC 2 cut(s) 328, 366
PspPI GGNCC 1 cut(s) 326
PsuI RGATCY 2 cut(s) 122, 364
RsaI GTAC 3 cut(s) 36, 227, 527
RsaNI GTAC 3 cut(s) 35, 226, 526
SaqAI TTAA 1 cut(s) 669
SatI GCNGC 3 cut(s) 51, 69, 591
Sau3AI GATC 2 cut(s) 122, 364
Sau96I GGNCC 1 cut(s) 326
SduI GDGCHC 1 cut(s) 377
SfaNI GCATC 1 cut(s) 826
SfcI CTRYAG 1 cut(s) 649
SmlI CTYRAG 2 cut(s) 446, 824
SmoI CTYRAG 2 cut(s) 446, 824
Sse9I AATT 1 cut(s) 572
SsiI CCGC 2 cut(s) 68, 591
SspI AATATT 1 cut(s) 781
SspMI CTAG 2 cut(s) 65, 594
StyI CCWWGG 1 cut(s) 795
TaaI ACNGT 2 cut(s) 152, 179
TaiI ACGT 4 cut(s) 612, 638, 660, 755
TasI AATT 1 cut(s) 572
TatI WGTACW 1 cut(s) 34
TauI GCSGC 2 cut(s) 71, 593
TfiI GAWTC 2 cut(s) 211, 289
Tru1I TTAA 1 cut(s) 669
Tru9I TTAA 1 cut(s) 669
TscAI CASTG 2 cut(s) 157, 184
TseI GCWGC 1 cut(s) 50
TspDTI ATGAA 2 cut(s) 430, 599
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 2 cut(s) 157, 184
XceI RCATGY 1 cut(s) 722
XcmI CCANNNNNNNNNTGG 1 cut(s) 388
XspI CTAG 2 cut(s) 65, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.