MD08G1034000.v1.1

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015873
Physical Location & Seq
Forward (+)
2398857 .. 2400780
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1034000.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGAGGAAGCTGTGCCCTAACCTTGACAATCAAGATGGCCTGGCTACCGTCCTCGAGGTGCCGATTCCGGAGGAAATGTTCACCAGCATGGGCAGCAGCGCCGAGTTGCAGTGGGCGAATCTCCGTTCTTCCATGAAAGCTCATCTCTCGTCAGGATCAAACAATGAGTTCATGGCATTGCTCAAGCTTGTGGGCTGTCCTCTCATCCCCCTTCAGGTTCATTGCGACAATGCACTCTCCCGCCCTATCAAAGACTGTTCAATCGAGGCTTCGACGGCGCATTATATAGTGCAACAATATGTGGCGGCGACCGGGGGACTGGCGGCGTTGAATTCGGTGAATAGCATGTGTGCCGTTGGGGAGGTGAAGATGGTGGGATCGGAAATGCACCAAGGTGATAACGATAGTGTACACGTTCAGAGCAAAGGCAATGGCGAGGTTGGTGGGTTTGTCCTATGGCAAAAAGACCCCGACCTGTGGTACTTGGAATTGTTTGTTTCCGGCTTCAAAGTCAGTGCTGGAAGCGATGGCAAGGTCGCATGGAATCAATCCGGCTCGCAACCTTGCCATGCCAATAAAGGGCTCCCAAGACCCCTCCGCCGCTTCTTTCAGGGTTTGGACCCAAGGAGAACAGCCAACTTGTTCCTAGACGCAGTATGCACAGGAGAGAAAAGCATCAACAAAGAAGAATGCTTCATCCTCAAGCTAGAGACTCCCACGGAAATACTCCAATCCCAAAGCACTCCCCAGACTGAAATAGTCCACCACACCATCTGGGGCTACTTCAGCCAGCGTACGGGACTGCTAATCCAGTTCGAGGACAGCAAGCTGGTCAAGATGAGGTCGCGAAACGACAACGTTTTCTGGGAGACGACGATGGAGACGGTGATCGGGGACTACAGATACGTCGACGGGATAAACATAGCGCACGCTGGCAAAACGTCGGCGTTGCTGTACAGGTACGGACACGCTTACAACCAGAAGAGGAGGATTGAGGAGACGTGGAAGATTGACGAGGTGGATTTCAATATTCGTGGGTTGTCCACGGATTGCTTTCTTCCTCCGGCGGATCTTAAGAGGGAAGATGGCGGTGGAGATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

368

Amino Acids

40.82

Weight (kDa)

5.72

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 116 - 355 1.1e-113 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015873)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75160
fragaria_vesca FvH4_2g39940
malus_domestica MD08G1034000.v1.1 MD15G1030800.v1.1
prunus_persica Prupe.1G381000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0300821
rosa_laevigata RLG00000011297
rosa_multiflora Rmu_co8409361.1_g000001 Rmu_sc0008947.1_g000004
rosa_roxburghii Rroxscaffold_7G00167380
rosa_rugosa Rorug06G0299300
rosa_samantha Rh6AG412400 Rh6BG419600 Rh6CG427100 Rh6DG412400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 58
AccI GTMKAC 1 cut(s) 909
AccII CGCG 1 cut(s) 847
AccIII TCCGGA 1 cut(s) 67
AciI CCGC 7 cut(s) 241, 305, 323, 598, 601, 1067, 1089
AclI AACGTT 1 cut(s) 858
AclWI GGATC 3 cut(s) 163, 385, 1077
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 2 cut(s) 197, 769
AfaI GTAC 5 cut(s) 411, 482, 796, 956, 962
AfiI CCNNNNNNNGG 5 cut(s) 214, 477, 579, 796, 817
AflII CTTAAG 1 cut(s) 1073
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 4 cut(s) 261, 331, 508, 1027
AjiI CACGTC 1 cut(s) 1002
AjnI CCWGG 1 cut(s) 39
AleI CACNNNNGTG 1 cut(s) 393
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
AluBI AGCT 5 cut(s) 10, 140, 187, 706, 829
AluI AGCT 5 cut(s) 10, 140, 187, 706, 829
Alw26I GTCTC 4 cut(s) 704, 863, 875, 992
AlwI GGATC 3 cut(s) 163, 385, 1077
Ama87I CYCGRG 1 cut(s) 53
Aor13HI TCCGGA 1 cut(s) 67
AoxI GGCC 1 cut(s) 37
ApeKI GCWGC 2 cut(s) 93, 96
ApoI RAATTY 1 cut(s) 331
AspLEI GCGC 3 cut(s) 101, 280, 928
AspS9I GGNCC 1 cut(s) 619
AsuC2I CCSGG 1 cut(s) 313
AsuHPI GGTGA 5 cut(s) 73, 349, 376, 407, 898
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 619
BaeGI GKGCMC 1 cut(s) 17
BanI GGYRCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 585
BbvI GCAGC 2 cut(s) 105, 108
BccI CCATC 6 cut(s) 29, 364, 521, 779, 871, 1079
BceAI ACGGC 2 cut(s) 291, 338
BciT130I CCWGG 1 cut(s) 41
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 4 cut(s) 704, 863, 875, 992
BfaI CTAG 2 cut(s) 647, 707
BfmI CTRYAG 1 cut(s) 898
BfoI RGCGCY 1 cut(s) 102
BfrI CTTAAG 1 cut(s) 1073
BisI GCNGC 5 cut(s) 94, 97, 306, 324, 601
BlsI GCNGC 5 cut(s) 95, 98, 307, 325, 602
Bme1390I CCNGG 2 cut(s) 41, 313
Bme18I GGWCC 1 cut(s) 619
BmeT110I CYCGRG 1 cut(s) 53
BmgBI CACGTC 1 cut(s) 1002
BmgT120I GGNCC 1 cut(s) 619
BmiI GGNNCC 3 cut(s) 60, 584, 621
BmrFI CCNGG 2 cut(s) 41, 313
BmsI GCATC 1 cut(s) 684
BpuEI CTTGAG 2 cut(s) 167, 686
BpuMI CCSGG 1 cut(s) 313
BsaJI CCNNGG 5 cut(s) 312, 391, 623, 717, 1044
BsaWI WCCGGW 1 cut(s) 67
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 477, 579, 796, 817
Bse1I ACTGG 2 cut(s) 324, 811
Bse3DI GCAATG 3 cut(s) 176, 220, 436
BseAI TCCGGA 1 cut(s) 67
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 5 cut(s) 312, 391, 623, 717, 1044
BseGI GGATG 2 cut(s) 204, 696
BseLI CCNNNNNNNGG 5 cut(s) 214, 477, 579, 796, 817
BseMI GCAATG 3 cut(s) 176, 220, 436
BseNI ACTGG 2 cut(s) 324, 811
BseRI GAGGAG 2 cut(s) 1000, 1010
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 2 cut(s) 105, 108
Bsh1236I CGCG 1 cut(s) 847
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 1 cut(s) 39
BshNI GGYRCC 1 cut(s) 58
BsiEI CGRYCG 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 5 cut(s) 68, 312, 501, 552, 1064
BsiWI CGTACG 1 cut(s) 794
BslFI GGGAC 3 cut(s) 330, 813, 908
BslI CCNNNNNNNGG 5 cut(s) 214, 477, 579, 796, 817
BsmAI GTCTC 4 cut(s) 704, 863, 875, 992
BsmBI CGTCTC 3 cut(s) 863, 875, 992
BsmFI GGGAC 3 cut(s) 330, 813, 908
BsmI GAATGC 1 cut(s) 695
BsnI GGCC 1 cut(s) 39
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 2 cut(s) 17, 585
Bsp13I TCCGGA 1 cut(s) 67
Bsp1407I TGTACA 2 cut(s) 409, 954
Bsp143I GATC 5 cut(s) 155, 377, 888, 1069, 1096
Bsp68I TCGCGA 1 cut(s) 847
BspACI CCGC 7 cut(s) 241, 305, 323, 598, 601, 1067, 1089
BspANI GGCC 1 cut(s) 39
BspEI TCCGGA 1 cut(s) 67
BspFNI CGCG 1 cut(s) 847
BspLI GGNNCC 3 cut(s) 60, 584, 621
BspPI GGATC 3 cut(s) 163, 385, 1077
BspT107I GGYRCC 1 cut(s) 58
BspTI CTTAAG 1 cut(s) 1073
BsrDI GCAATG 3 cut(s) 176, 220, 436
BsrGI TGTACA 2 cut(s) 409, 954
BsrI ACTGG 2 cut(s) 324, 811
BssECI CCNNGG 5 cut(s) 312, 391, 623, 717, 1044
BssMI GATC 5 cut(s) 155, 377, 888, 1069, 1096
BssT1I CCWWGG 2 cut(s) 391, 623
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 3 cut(s) 49, 257, 886
Bst6I CTCTTC 1 cut(s) 977
BstAFI CTTAAG 1 cut(s) 1073
BstAUI TGTACA 2 cut(s) 409, 954
BstC8I GCNNGC 5 cut(s) 557, 791, 827, 930, 934
BstDSI CCRYGG 2 cut(s) 717, 1044
BstF5I GGATG 2 cut(s) 204, 696
BstFNI CGCG 1 cut(s) 847
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 3 cut(s) 101, 280, 928
BstKTI GATC 5 cut(s) 158, 380, 891, 1072, 1099
BstMAI GTCTC 4 cut(s) 704, 863, 875, 992
BstMBI GATC 5 cut(s) 155, 377, 888, 1069, 1096
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 3 cut(s) 93, 275, 786
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 2 cut(s) 39, 311
BstSFI CTRYAG 1 cut(s) 898
BstSLI GKGCMC 1 cut(s) 17
BstUI CGCG 1 cut(s) 847
BstV1I GCAGC 2 cut(s) 105, 108
BstX2I RGATCY 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 1069
BsuRI GGCC 1 cut(s) 39
BtgI CCRYGG 2 cut(s) 717, 1044
BtgZI GCGATG 1 cut(s) 540
BtrI CACGTC 1 cut(s) 1002
BtsCI GGATG 2 cut(s) 204, 696
BtsI GCAGTG 1 cut(s) 116
BtsIMutI CAGTG 2 cut(s) 116, 520
BtuMI TCGCGA 1 cut(s) 847
Cac8I GCNNGC 5 cut(s) 557, 791, 827, 930, 934
CfoI GCGC 3 cut(s) 101, 280, 928
Cfr13I GGNCC 1 cut(s) 619
CseI GACGC 1 cut(s) 659
Csp6I GTAC 5 cut(s) 410, 481, 795, 955, 961
CspCI CAANNNNNGTGG 2 cut(s) 1015, 1050
CviAII CATG 6 cut(s) 88, 133, 172, 346, 540, 569
CviQI GTAC 5 cut(s) 410, 481, 795, 955, 961
DpnI GATC 5 cut(s) 157, 379, 890, 1071, 1098
DpnII GATC 5 cut(s) 155, 377, 888, 1069, 1096
Eam1104I CTCTTC 1 cut(s) 977
EarI CTCTTC 1 cut(s) 977
EciI GGCGGA 2 cut(s) 587, 1082
Eco130I CCWWGG 2 cut(s) 391, 623
Eco24I GRGCYC 1 cut(s) 585
Eco47I GGWCC 1 cut(s) 619
Eco57I CTGAAG 2 cut(s) 197, 769
Eco88I CYCGRG 1 cut(s) 53
EcoRI GAATTC 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 2 cut(s) 391, 623
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 2 cut(s) 391, 623
Esp3I CGTCTC 3 cut(s) 863, 875, 992
FaeI CATG 6 cut(s) 91, 136, 175, 349, 543, 572
FaqI GGGAC 3 cut(s) 330, 813, 908
FatI CATG 6 cut(s) 87, 132, 171, 345, 539, 568
FauI CCCGC 1 cut(s) 248
FblI GTMKAC 1 cut(s) 909
Fnu4HI GCNGC 5 cut(s) 94, 97, 306, 324, 601
FokI GGATG 2 cut(s) 191, 683
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 5 cut(s) 94, 97, 306, 324, 601
FspBI CTAG 2 cut(s) 647, 707
GlaI GCGC 3 cut(s) 100, 279, 927
GluI GCNGC 5 cut(s) 94, 97, 306, 324, 601
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 1 cut(s) 39
HapII CCGG 5 cut(s) 68, 312, 501, 552, 1064
HgaI GACGC 1 cut(s) 659
HhaI GCGC 3 cut(s) 101, 280, 928
Hin1II CATG 6 cut(s) 91, 136, 175, 349, 543, 572
Hin6I GCGC 3 cut(s) 99, 278, 926
HinP1I GCGC 3 cut(s) 99, 278, 926
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 4 cut(s) 64, 118, 544, 712
HpaII CCGG 5 cut(s) 68, 312, 501, 552, 1064
HphI GGTGA 5 cut(s) 73, 349, 376, 407, 898
Hpy166II GTNNAC 6 cut(s) 81, 410, 412, 763, 910, 1044
Hpy188I TCNGA 2 cut(s) 382, 420
Hpy188III TCNNGA 5 cut(s) 32, 68, 153, 835, 846
Hpy8I GTNNAC 6 cut(s) 81, 410, 412, 763, 910, 1044
Hpy99I CGWCG 5 cut(s) 277, 877, 911, 914, 946
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 3 cut(s) 49, 257, 886
HpyCH4IV ACGT 5 cut(s) 414, 858, 906, 941, 1001
HpyCH4V TGCA 5 cut(s) 109, 233, 292, 388, 660
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 275, 786
HpySE526I ACGT 5 cut(s) 414, 858, 906, 941, 1001
Hsp92II CATG 6 cut(s) 91, 136, 175, 349, 543, 572
HspAI GCGC 3 cut(s) 99, 278, 926
Kpn2I TCCGGA 1 cut(s) 67
Kzo9I GATC 5 cut(s) 155, 377, 888, 1069, 1096
LmnI GCTCC 1 cut(s) 588
Lsp1109I GCAGC 2 cut(s) 105, 108
LweI GCATC 1 cut(s) 684
MaeI CTAG 2 cut(s) 647, 707
MaeII ACGT 5 cut(s) 414, 858, 906, 941, 1001
MalI GATC 5 cut(s) 157, 379, 890, 1071, 1098
MboI GATC 5 cut(s) 155, 377, 888, 1069, 1096
MboII GAAGA 7 cut(s) 120, 379, 698, 994, 1018, 1049, 1094
MflI RGATCY 1 cut(s) 1069
MhlI GDGCHC 2 cut(s) 17, 585
MluCI AATT 2 cut(s) 331, 488
MlyI GAGTC 1 cut(s) 706
MroI TCCGGA 1 cut(s) 67
MseI TTAA 1 cut(s) 1074
MslI CAYNNNNRTG 2 cut(s) 86, 393
MspCI CTTAAG 1 cut(s) 1073
MspI CCGG 5 cut(s) 68, 312, 501, 552, 1064
MspR9I CCNGG 2 cut(s) 41, 313
Mva1269I GAATGC 1 cut(s) 695
MvaI CCWGG 1 cut(s) 41
MvnI CGCG 1 cut(s) 847
MwoI GCNNNNNNNGC 3 cut(s) 93, 275, 786
NciI CCSGG 1 cut(s) 313
NdeII GATC 5 cut(s) 155, 377, 888, 1069, 1096
NlaIII CATG 6 cut(s) 91, 136, 175, 349, 543, 572
NlaIV GGNNCC 3 cut(s) 60, 584, 621
NmeAIII GCCGAG 1 cut(s) 127
NruI TCGCGA 1 cut(s) 847
NspI RCATGY 1 cut(s) 349
OliI CACNNNNGTG 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 53
PctI GAATGC 1 cut(s) 695
PfeI GAWTC 3 cut(s) 64, 118, 544
Pfl23II CGTACG 1 cut(s) 794
PkrI GCNGC 5 cut(s) 95, 98, 307, 325, 602
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
Psp1406I AACGTT 1 cut(s) 858
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspLI CGTACG 1 cut(s) 794
PspN4I GGNNCC 3 cut(s) 60, 584, 621
PspPI GGNCC 1 cut(s) 619
PspXI VCTCGAGB 1 cut(s) 53
PsuI RGATCY 1 cut(s) 1069
RruI TCGCGA 1 cut(s) 847
RsaI GTAC 5 cut(s) 411, 482, 796, 956, 962
RsaNI GTAC 5 cut(s) 410, 481, 795, 955, 961
RseI CAYNNNNRTG 2 cut(s) 86, 393
SalI GTCGAC 1 cut(s) 908
SaqAI TTAA 1 cut(s) 1074
SatI GCNGC 5 cut(s) 94, 97, 306, 324, 601
Sau3AI GATC 5 cut(s) 155, 377, 888, 1069, 1096
Sau96I GGNCC 1 cut(s) 619
SchI GAGTC 1 cut(s) 706
ScrFI CCNGG 2 cut(s) 41, 313
SduI GDGCHC 2 cut(s) 17, 585
SfaNI GCATC 1 cut(s) 684
SfcI CTRYAG 1 cut(s) 898
Sfr274I CTCGAG 1 cut(s) 53
SgrDI CGTCGACG 1 cut(s) 908
SinI GGWCC 1 cut(s) 619
SlaI CTCGAG 1 cut(s) 53
SmiMI CAYNNNNRTG 2 cut(s) 86, 393
SmlI CTYRAG 4 cut(s) 53, 182, 701, 1073
SmoI CTYRAG 4 cut(s) 53, 182, 701, 1073
Sse9I AATT 2 cut(s) 331, 488
SsiI CCGC 7 cut(s) 241, 305, 323, 598, 601, 1067, 1089
SspI AATATT 1 cut(s) 1030
SspMI CTAG 2 cut(s) 647, 707
StyD4I CCNGG 2 cut(s) 39, 311
StyI CCWWGG 2 cut(s) 391, 623
TaaI ACNGT 3 cut(s) 49, 257, 886
TaiI ACGT 5 cut(s) 417, 861, 909, 944, 1004
TaqI TCGA 5 cut(s) 54, 264, 272, 816, 909
TasI AATT 2 cut(s) 331, 488
TatI WGTACW 2 cut(s) 409, 954
TauI GCSGC 3 cut(s) 308, 326, 603
TfiI GAWTC 3 cut(s) 64, 118, 544
Tru1I TTAA 1 cut(s) 1074
Tru9I TTAA 1 cut(s) 1074
TscAI CASTG 2 cut(s) 116, 520
TseI GCWGC 2 cut(s) 93, 96
TspDTI ATGAA 4 cut(s) 149, 160, 209, 685
TspGWI ACGGA 4 cut(s) 113, 734, 978, 1061
TspRI CASTG 2 cut(s) 116, 520
Vha464I CTTAAG 1 cut(s) 1073
VpaK11BI GGWCC 1 cut(s) 619
XapI RAATTY 1 cut(s) 331
XceI RCATGY 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 53
XmiI GTMKAC 1 cut(s) 909
XspI CTAG 2 cut(s) 647, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.