FvH4_2g40880

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014679
Physical Location & Seq
Forward (+)
29032018 .. 29034988
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40880.t1

Sequence Viewer

Length: 1134 bp
ATGGCTACCCTAACAACATTCATGTCATCAGTGATTCTCATTCTCGTAATCCTTTGCCCTAGTATTCATGCTAAACCTAGTAAAGGAATCAGAGCAGCCTATTGGCCGTCGTTCAATTCATTCCCCGCTTCCTCTATACAAACCTCTCTTTTCACCCACATTTACTACGCCTTCCTCCTTCCCGATCCCGCCACTTTCAAACTCACCATCACCTCCGAGGACCAAACCAAGCTACCCGACTTCATCTCATCCCTCCGCGCCCTAAACCCTCCCGTCAAAACCCTCCTCTCCATCGGAGGCGGCGGTAACAACGCCACAGTGTTCTCGGAGATGGTTAGCACCAAACAGAGCCGTCAAGTCTTCATCAACTCCTCCATCGAAATAGCCCGGAAGTACGGCTTCCATGGTGTCGACTTGGATTGGGAGTTTCCGGCTGACGTGGTCGACATGTCAAACCTCGCTCTGTTGTACAAGCAGTGGAGACAATCTCTAGACCAGGAAGCTAAAGCTAGCGGCAAGCCACGTCTGCTCTTAACCTCCGCCGTGTATTACGCTTCGGAATTTACAGTCTACGGCGGGCCGAGATCATACCCGGCCGGAGCAATAAGTAAGTACTTGGATTGGGCGAGTCCGATGTGCTTTGACTATCACGGCTCTTGGGAAAACTTCACCGGCGCACATGCAGCCTTGTATGACTCCAAGAGTAATATCAGTACTGACTACGGAATCCGTTCGTGGATTCAGGCGGGTGTACCGGCCAAGAAGCTTGTGATGGGCCTACCTCTTTATGGGAAGAGTTGGACGCTCCGGGACCAGAAGGTGAATGGTGTCGGGGCCGCGGCGGTGGGCGTTGGGCCCGGTGATGGTGGCATTTTGCTGTATCATCAGATAGTTGACTTGAACGCTCGAACTAACGCAACGGTTGTGTTTGACAACCAGACAGTGTCTTACTATTCCTACTCGGGTAGCACTTGGATTGGGTACGATGATGTTGGATCTGTCAGGTTGAAGGTTCGGTTTGCCAAGAAAATGGGCTTGGGTGGGTATTTCTTTTGGGCTCTGGGCCAGGACAAAGATTGGATCCTATCACACCAAGCTTCAGATACTTGGAAATATGGCGGGGGATCGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

378

Amino Acids

41.42

Weight (kDa)

8.9

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 32 - 357 7.2e-76 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014679)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g40880 FvH4_2g40880
malus_domestica MD08G1059100.v1.1 MD14G1173400.v1.1 MD15G1037300.v1.1
prunus_persica Prupe.1G391300_v2.0.a1
pyrus_communis pycom15g03440
rosa_chinensis RchiOBHm_Chr6g0299691
rosa_laevigata RLG00000011396
rosa_multiflora Rmu_sc0002895.1_g000010
rosa_roxburghii Rroxscaffold_7G00168440
rosa_rugosa Rorug06G0291600
rosa_samantha Rh6AG402400 Rh6BG410900 Rh6CG416700 Rh6DG403800
rosa_wichuraiana Rw6G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 411, 444, 570
AccII CGCG 2 cut(s) 258, 839
AclWI GGATC 5 cut(s) 179, 1003, 1075, 1088, 1132
AcoI YGGCCR 3 cut(s) 104, 594, 756
AcsI RAATTY 1 cut(s) 560
AcuI CTGAAG 1 cut(s) 1083
AfaI GTAC 6 cut(s) 395, 470, 614, 715, 753, 983
AfiI CCNNNNNNNGG 3 cut(s) 83, 788, 863
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 4 cut(s) 115, 199, 901, 1009
AjiI CACGTC 2 cut(s) 439, 524
AjnI CCWGG 2 cut(s) 495, 1065
AjuI GAANNNNNNNTTGG 2 cut(s) 1019, 1051
AluBI AGCT 5 cut(s) 232, 503, 509, 766, 1097
AluI AGCT 5 cut(s) 232, 503, 509, 766, 1097
Alw26I GTCTC 1 cut(s) 475
AlwI GGATC 5 cut(s) 179, 1003, 1075, 1088, 1132
Ama87I CYCGRG 1 cut(s) 961
AoxI GGCC 8 cut(s) 104, 578, 594, 756, 775, 834, 854, 1063
ApaI GGGCCC 1 cut(s) 858
ApeKI GCWGC 2 cut(s) 95, 683
ApoI RAATTY 1 cut(s) 560
Asp700I GAANNNNTTC 1 cut(s) 730
AspLEI GCGC 2 cut(s) 260, 677
AspS9I GGNCC 8 cut(s) 220, 578, 775, 811, 834, 854, 855, 1063
AsuC2I CCSGG 4 cut(s) 388, 593, 809, 858
AsuHPI GGTGA 6 cut(s) 145, 196, 202, 661, 832, 872
AsuNHI GCTAGC 1 cut(s) 509
AvaI CYCGRG 1 cut(s) 961
AvaII GGWCC 2 cut(s) 220, 811
BaeGI GKGCMC 1 cut(s) 858
BamHI GGATCC 1 cut(s) 1080
BanII GRGCYC 2 cut(s) 858, 1060
BbsI GAAGAC 1 cut(s) 352
BbvI GCAGC 2 cut(s) 107, 695
BccI CCATC 6 cut(s) 215, 299, 325, 383, 766, 857
BceAI ACGGC 6 cut(s) 91, 336, 412, 527, 589, 667
BciT130I CCWGG 2 cut(s) 497, 1067
BcnI CCSGG 4 cut(s) 388, 593, 809, 858
BcoDI GTCTC 1 cut(s) 475
BfaI CTAG 4 cut(s) 60, 78, 491, 510
BisI GCNGC 6 cut(s) 96, 301, 514, 684, 837, 840
BlsI GCNGC 6 cut(s) 97, 302, 515, 685, 838, 841
BmcAI AGTACT 2 cut(s) 614, 715
Bme1390I CCNGG 6 cut(s) 388, 497, 593, 809, 858, 1067
Bme18I GGWCC 2 cut(s) 220, 811
BmeT110I CYCGRG 1 cut(s) 961
BmgBI CACGTC 2 cut(s) 439, 524
BmgT120I GGNCC 8 cut(s) 220, 578, 775, 811, 834, 854, 855, 1063
BmiI GGNNCC 4 cut(s) 812, 835, 856, 1082
BmrFI CCNGG 6 cut(s) 388, 497, 593, 809, 858, 1067
BmtI GCTAGC 1 cut(s) 513
BpiI GAAGAC 1 cut(s) 352
BplI GAGNNNNNCTC 2 cut(s) 472, 504
BpuMI CCSGG 4 cut(s) 388, 593, 809, 858
BsaJI CCNNGG 3 cut(s) 216, 403, 837
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 788, 863
Bse118I RCCGGY 2 cut(s) 671, 754
BseBI CCWGG 2 cut(s) 497, 1067
BseDI CCNNGG 3 cut(s) 216, 403, 837
BseGI GGATG 1 cut(s) 248
BseLI CCNNNNNNNGG 3 cut(s) 83, 788, 863
BseRI GAGGAG 2 cut(s) 275, 361
BseSI GKGCMC 1 cut(s) 858
BseX3I CGGCCG 1 cut(s) 594
BseXI GCAGC 2 cut(s) 107, 695
Bsh1236I CGCG 2 cut(s) 258, 839
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 8 cut(s) 106, 580, 596, 758, 777, 836, 856, 1065
BsiEI CGRYCG 1 cut(s) 597
BsiHKCI CYCGRG 1 cut(s) 961
BsiSI CCGG 8 cut(s) 388, 431, 593, 597, 672, 755, 808, 858
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 3 cut(s) 83, 788, 863
BsmAI GTCTC 1 cut(s) 475
BsmFI GGGAC 1 cut(s) 824
BsnI GGCC 8 cut(s) 106, 580, 596, 758, 777, 836, 856, 1065
BsoBI CYCGRG 1 cut(s) 961
Bsp120I GGGCCC 1 cut(s) 854
Bsp1286I GDGCHC 2 cut(s) 858, 1060
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 5 cut(s) 184, 584, 995, 1080, 1124
Bsp19I CCATGG 1 cut(s) 403
BspANI GGCC 8 cut(s) 106, 580, 596, 758, 777, 836, 856, 1065
BspFNI CGCG 2 cut(s) 258, 839
BspLI GGNNCC 4 cut(s) 812, 835, 856, 1082
BspOI GCTAGC 1 cut(s) 513
BspPI GGATC 5 cut(s) 179, 1003, 1075, 1088, 1132
BsrFI RCCGGY 2 cut(s) 671, 754
BsrGI TGTACA 1 cut(s) 468
BssAI RCCGGY 2 cut(s) 671, 754
BssECI CCNNGG 3 cut(s) 216, 403, 837
BssMI GATC 5 cut(s) 184, 584, 995, 1080, 1124
BssT1I CCWWGG 1 cut(s) 403
Bst2UI CCWGG 2 cut(s) 497, 1067
Bst4CI ACNGT 4 cut(s) 319, 568, 922, 943
Bst6I CTCTTC 1 cut(s) 788
BstAUI TGTACA 1 cut(s) 468
BstC8I GCNNGC 3 cut(s) 511, 518, 578
BstDSI CCRYGG 2 cut(s) 403, 837
BstENI CCTNNNNNAGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 248
BstFNI CGCG 2 cut(s) 258, 839
BstHHI GCGC 2 cut(s) 260, 677
BstKTI GATC 5 cut(s) 187, 587, 998, 1083, 1127
BstMAI GTCTC 1 cut(s) 475
BstMBI GATC 5 cut(s) 184, 584, 995, 1080, 1124
BstMCI CGRYCG 1 cut(s) 597
BstMWI GCNNNNNNNGC 2 cut(s) 526, 683
BstNI CCWGG 2 cut(s) 497, 1067
BstNSI RCATGY 2 cut(s) 451, 683
BstSCI CCNGG 6 cut(s) 386, 495, 591, 807, 856, 1065
BstSLI GKGCMC 1 cut(s) 858
BstUI CGCG 2 cut(s) 258, 839
BstV1I GCAGC 2 cut(s) 107, 695
BstV2I GAAGAC 1 cut(s) 352
BstX2I RGATCY 2 cut(s) 995, 1080
BstXI CCANNNNNNTGG 1 cut(s) 1030
BstYI RGATCY 2 cut(s) 995, 1080
BstZI CGGCCG 1 cut(s) 594
BsuRI GGCC 8 cut(s) 106, 580, 596, 758, 777, 836, 856, 1065
BtgI CCRYGG 2 cut(s) 403, 837
BtrI CACGTC 2 cut(s) 439, 524
BtsCI GGATG 1 cut(s) 248
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 4 cut(s) 36, 324, 482, 948
Cac8I GCNNGC 3 cut(s) 511, 518, 578
CfoI GCGC 2 cut(s) 260, 677
Cfr10I RCCGGY 2 cut(s) 671, 754
Cfr13I GGNCC 8 cut(s) 220, 578, 775, 811, 834, 854, 855, 1063
Cfr42I CCGCGG 1 cut(s) 840
CseI GACGC 1 cut(s) 811
Csp6I GTAC 6 cut(s) 394, 469, 613, 714, 752, 982
CviAII CATG 5 cut(s) 22, 68, 404, 448, 680
CviQI GTAC 6 cut(s) 394, 469, 613, 714, 752, 982
DpnI GATC 5 cut(s) 186, 586, 997, 1082, 1126
DpnII GATC 5 cut(s) 184, 584, 995, 1080, 1124
EaeI YGGCCR 3 cut(s) 104, 594, 756
EagI CGGCCG 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 788
EarI CTCTTC 1 cut(s) 788
EciI GGCGGA 1 cut(s) 529
EclXI CGGCCG 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 403
Eco24I GRGCYC 2 cut(s) 858, 1060
Eco47I GGWCC 2 cut(s) 220, 811
Eco52I CGGCCG 1 cut(s) 594
Eco57I CTGAAG 1 cut(s) 1083
Eco88I CYCGRG 1 cut(s) 961
EcoNI CCTNNNNNAGG 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 495, 1065
EcoT14I CCWWGG 1 cut(s) 403
EcoT38I GRGCYC 2 cut(s) 858, 1060
ErhI CCWWGG 1 cut(s) 403
FaeI CATG 5 cut(s) 25, 71, 407, 451, 683
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FaqI GGGAC 1 cut(s) 824
FatI CATG 5 cut(s) 21, 67, 403, 447, 679
FauI CCCGC 5 cut(s) 133, 196, 569, 739, 1112
FblI GTMKAC 3 cut(s) 411, 444, 570
Fnu4HI GCNGC 6 cut(s) 96, 301, 514, 684, 837, 840
FokI GGATG 1 cut(s) 235
FriOI GRGCYC 2 cut(s) 858, 1060
Fsp4HI GCNGC 6 cut(s) 96, 301, 514, 684, 837, 840
FspBI CTAG 4 cut(s) 60, 78, 491, 510
GlaI GCGC 2 cut(s) 259, 676
GluI GCNGC 6 cut(s) 96, 301, 514, 684, 837, 840
HaeIII GGCC 8 cut(s) 106, 580, 596, 758, 777, 836, 856, 1065
HapII CCGG 8 cut(s) 388, 431, 593, 597, 672, 755, 808, 858
HgaI GACGC 1 cut(s) 811
HhaI GCGC 2 cut(s) 260, 677
Hin1II CATG 5 cut(s) 25, 71, 407, 451, 683
Hin6I GCGC 2 cut(s) 258, 675
HinP1I GCGC 2 cut(s) 258, 675
HincII GTYRAC 3 cut(s) 412, 445, 895
HindII GTYRAC 3 cut(s) 412, 445, 895
HindIII AAGCTT 2 cut(s) 764, 1095
HinfI GANTC 6 cut(s) 34, 87, 628, 695, 726, 739
HpaII CCGG 8 cut(s) 388, 431, 593, 597, 672, 755, 808, 858
HphI GGTGA 6 cut(s) 145, 196, 202, 661, 832, 872
Hpy166II GTNNAC 5 cut(s) 412, 445, 571, 752, 895
Hpy188I TCNGA 9 cut(s) 92, 217, 296, 328, 559, 633, 888, 1102, 1133
Hpy188III TCNNGA 2 cut(s) 182, 491
Hpy8I GTNNAC 5 cut(s) 412, 445, 571, 752, 895
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 4 cut(s) 181, 188, 811, 1003
HpyCH4III ACNGT 4 cut(s) 319, 568, 922, 943
HpyCH4IV ACGT 2 cut(s) 438, 523
HpyCH4V TGCA 1 cut(s) 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 526, 683
HpySE526I ACGT 2 cut(s) 438, 523
Hsp92II CATG 5 cut(s) 25, 71, 407, 451, 683
HspAI GCGC 2 cut(s) 258, 675
KspI CCGCGG 1 cut(s) 840
Kzo9I GATC 5 cut(s) 184, 584, 995, 1080, 1124
LmnI GCTCC 2 cut(s) 599, 810
Lsp1109I GCAGC 2 cut(s) 107, 695
MaeI CTAG 4 cut(s) 60, 78, 491, 510
MaeII ACGT 2 cut(s) 438, 523
MaeIII GTNAC 1 cut(s) 305
MalI GATC 5 cut(s) 186, 586, 997, 1082, 1126
MboI GATC 5 cut(s) 184, 584, 995, 1080, 1124
MboII GAAGA 2 cut(s) 352, 805
MflI RGATCY 2 cut(s) 995, 1080
MhlI GDGCHC 2 cut(s) 858, 1060
MluCI AATT 2 cut(s) 115, 560
MlyI GAGTC 2 cut(s) 637, 689
MmeI TCCRAC 2 cut(s) 779, 973
MroXI GAANNNNTTC 1 cut(s) 730
MseI TTAA 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 839
MspI CCGG 8 cut(s) 388, 431, 593, 597, 672, 755, 808, 858
MspR9I CCNGG 6 cut(s) 388, 497, 593, 809, 858, 1067
MvaI CCWGG 2 cut(s) 497, 1067
MvnI CGCG 2 cut(s) 258, 839
MwoI GCNNNNNNNGC 2 cut(s) 526, 683
NciI CCSGG 4 cut(s) 388, 593, 809, 858
NcoI CCATGG 1 cut(s) 403
NdeII GATC 5 cut(s) 184, 584, 995, 1080, 1124
NheI GCTAGC 1 cut(s) 509
NlaIII CATG 5 cut(s) 25, 71, 407, 451, 683
NlaIV GGNNCC 4 cut(s) 812, 835, 856, 1082
NmeAIII GCCGAG 1 cut(s) 606
NspI RCATGY 2 cut(s) 451, 683
PciI ACATGT 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 730
PfeI GAWTC 4 cut(s) 34, 87, 726, 739
PflFI GACNNNGTC 2 cut(s) 440, 943
PfoI TCCNGGA 1 cut(s) 807
PkrI GCNGC 6 cut(s) 97, 302, 515, 685, 838, 841
PleI GAGTC 2 cut(s) 636, 689
PpsI GAGTC 2 cut(s) 636, 689
PscI ACATGT 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 495, 1065
PspGI CCWGG 2 cut(s) 495, 1065
PspN4I GGNNCC 4 cut(s) 812, 835, 856, 1082
PspOMI GGGCCC 1 cut(s) 854
PspPI GGNCC 8 cut(s) 220, 578, 775, 811, 834, 854, 855, 1063
PsuI RGATCY 2 cut(s) 995, 1080
PsyI GACNNNGTC 2 cut(s) 440, 943
RsaI GTAC 6 cut(s) 395, 470, 614, 715, 753, 983
RsaNI GTAC 6 cut(s) 394, 469, 613, 714, 752, 982
SacII CCGCGG 1 cut(s) 840
SalI GTCGAC 2 cut(s) 410, 443
SaqAI TTAA 1 cut(s) 533
SatI GCNGC 6 cut(s) 96, 301, 514, 684, 837, 840
Sau3AI GATC 5 cut(s) 184, 584, 995, 1080, 1124
Sau96I GGNCC 8 cut(s) 220, 578, 775, 811, 834, 854, 855, 1063
ScaI AGTACT 2 cut(s) 614, 715
SchI GAGTC 2 cut(s) 637, 689
ScrFI CCNGG 6 cut(s) 388, 497, 593, 809, 858, 1067
SduI GDGCHC 2 cut(s) 858, 1060
Sfr303I CCGCGG 1 cut(s) 840
SgrAI CRCCGGYG 1 cut(s) 671
SgrBI CCGCGG 1 cut(s) 840
SinI GGWCC 2 cut(s) 220, 811
Sse9I AATT 2 cut(s) 115, 560
SspMI CTAG 4 cut(s) 60, 78, 491, 510
StyD4I CCNGG 6 cut(s) 386, 495, 591, 807, 856, 1065
StyI CCWWGG 1 cut(s) 403
TaaI ACNGT 4 cut(s) 319, 568, 922, 943
TaiI ACGT 2 cut(s) 441, 526
TaqI TCGA 4 cut(s) 378, 411, 444, 907
TaqII GACCGA 1 cut(s) 1117
TasI AATT 2 cut(s) 115, 560
TatI WGTACW 3 cut(s) 468, 612, 713
TauI GCSGC 4 cut(s) 303, 516, 839, 842
TfiI GAWTC 4 cut(s) 34, 87, 726, 739
Tru1I TTAA 1 cut(s) 533
Tru9I TTAA 1 cut(s) 533
TscAI CASTG 4 cut(s) 36, 324, 482, 948
TseI GCWGC 2 cut(s) 95, 683
TspDTI ATGAA 5 cut(s) 10, 56, 108, 232, 352
TspGWI ACGGA 2 cut(s) 719, 738
TspRI CASTG 4 cut(s) 36, 324, 482, 948
Tth111I GACNNNGTC 2 cut(s) 440, 943
VpaK11BI GGWCC 2 cut(s) 220, 811
XagI CCTNNNNNAGG 1 cut(s) 81
XapI RAATTY 1 cut(s) 560
XbaI TCTAGA 1 cut(s) 490
XceI RCATGY 2 cut(s) 451, 683
XcmI CCANNNNNNNNNTGG 1 cut(s) 821
XmiI GTMKAC 3 cut(s) 411, 444, 570
XmnI GAANNNNTTC 1 cut(s) 730
XspI CTAG 4 cut(s) 60, 78, 491, 510
ZrmI AGTACT 2 cut(s) 614, 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.