RLG00000011396

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014679
Physical Location & Seq
Forward (+)
9108230 .. 9110356
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011396

Sequence Viewer

Length: 1146 bp
ATGGGTACCCCAACAACATTCATGTCATTGTTTCTCATTCTCGTAATCCTTGGCACTAGTACTGTAGCAAATGGTGATCATCCAGATCATGCTAAACCTAGTAAAGGAATCAGAGCAGCCTATTGGCCCTCGTTCAATTCCTTCCCTGCTTCCTCTATACAAACCTCTCTTTTCACCCACATTTACTACGCCTTCCTCCTACCCGATCCCGCCACTTTCAAACTCAGCATCACCTCAGACGACCAAACCAAGTTACCCGAGTTCATCGGGTCGCTCCGCAACCTAACCCCGCCCGTGAAAACCCTACTCTCCATCGGAGGCGGCGGTAGCAACGCAACAGTATTCTCGGAGATGGTTAGCACCAAACAGAGCCGTCAAGTATTCATCAACTCCTCCATTGAAGTAGCCCGGAAATACGGGTTCCAAGGTGTTGACTTGGATTGGGAGTTTCCGGCTAACGTGGTGGACATGTCAAACCTAGCTCTGTTATACAAGCAGTGGAGAAATTCTCTAGAAGAGGAAGCTAAGGCTAGCGGCAAGCCACGCCTGCTTTTAACCTCCGCCGTGTATTACGCTTCGGAATTTCAAGTTTTCGGCGGGCCGAGATCATACCCGGCCGGCGCAATGAGTAAATACTTGGACTGGGCTAGTCCAATGTGCTTTGACTATCACGGCTCGTGGGAGAACTTCACCGGCGAACATTCGGCCTTGTATGATTCCAAGAGTAACATCAGTACTAACTACGGAATCAGGTCGTGGATCCAAGCCGGTGTACCGGCCAAGAAGCTTGTGATGGGCCTACCTCTATACGGGAAGAGTTGGACGCTCCGGGACCCGAAAGTGAATGGGGTCGGGGCGCCGGCGGTGGGCGTGGGGCCAGGGGATGATGGTATTTTGCTTTATTACCAGATAGTTGAGTTGAATGCTCAGAGTAACGCAACAGTTGTGTTTGACAAGCAAACCAAGTCTTATTATTCCTACTCGGGTACCACTTGGATCGGGTATGAGGATGTTCGATCCGTCGCTTTGAAGGTTCGGTTTGCCAAGTCACTGGGCTTGGGTGGGTACTTCTTTTGGGCTCTGGGCCAGGACAAAGATTGGACCCTGTCACAGAAAGGTAAGGCACTATTTGATTTTGATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

382

Amino Acids

42.0

Weight (kDa)

8.33

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 39 - 364 1.4e-74 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014679)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g40880 FvH4_2g40880
malus_domestica MD08G1059100.v1.1 MD14G1173400.v1.1 MD15G1037300.v1.1
prunus_persica Prupe.1G391300_v2.0.a1
pyrus_communis pycom15g03440
rosa_chinensis RchiOBHm_Chr6g0299691
rosa_laevigata RLG00000011396
rosa_multiflora Rmu_sc0002895.1_g000010
rosa_roxburghii Rroxscaffold_7G00168440
rosa_rugosa Rorug06G0291600
rosa_samantha Rh6AG402400 Rh6BG410900 Rh6CG416700 Rh6DG403800
rosa_wichuraiana Rw6G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 5, 986
AccB1I GGYRCC 3 cut(s) 5, 856, 986
AciI CCGC 9 cut(s) 210, 277, 290, 321, 324, 534, 561, 597, 863
AclWI GGATC 5 cut(s) 200, 754, 767, 1004, 1011
AcoI YGGCCR 2 cut(s) 615, 777
AcsI RAATTY 2 cut(s) 505, 581
AcyI GRCGYC 1 cut(s) 857
AfaI GTAC 6 cut(s) 7, 61, 736, 774, 988, 1067
AfiI CCNNNNNNNGG 3 cut(s) 104, 809, 866
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 6 cut(s) 136, 220, 401, 587, 922, 1030
AhlI ACTAGT 1 cut(s) 56
AjnI CCWGG 2 cut(s) 877, 1086
AluBI AGCT 3 cut(s) 482, 524, 787
AluI AGCT 3 cut(s) 482, 524, 787
AlwI GGATC 5 cut(s) 200, 754, 767, 1004, 1011
Ama87I CYCGRG 2 cut(s) 257, 982
AoxI GGCC 8 cut(s) 125, 599, 615, 705, 777, 796, 875, 1084
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 2 cut(s) 505, 581
Asp718I GGTACC 2 cut(s) 5, 986
AspLEI GCGC 2 cut(s) 623, 859
AspS9I GGNCC 7 cut(s) 126, 599, 796, 832, 875, 1084, 1101
AsuC2I CCSGG 3 cut(s) 409, 614, 830
AsuHPI GGTGA 4 cut(s) 86, 166, 223, 682
AsuNHI GCTAGC 1 cut(s) 530
AvaI CYCGRG 2 cut(s) 257, 982
AvaII GGWCC 2 cut(s) 832, 1101
BaeI ACNNNNGTAYC 2 cut(s) 970, 1003
BamHI GGATCC 1 cut(s) 759
BanI GGYRCC 3 cut(s) 5, 856, 986
BanII GRGCYC 1 cut(s) 1081
BauI CACGAG 1 cut(s) 676
BbvI GCAGC 1 cut(s) 128
BccI CCATC 4 cut(s) 320, 346, 787, 881
BceAI ACGGC 3 cut(s) 357, 548, 688
BciT130I CCWGG 2 cut(s) 879, 1088
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 3 cut(s) 409, 614, 830
BcuI ACTAGT 1 cut(s) 56
BfaI CTAG 6 cut(s) 57, 99, 479, 512, 531, 648
BfmI CTRYAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 860
BisI GCNGC 3 cut(s) 117, 322, 535
BlsI GCNGC 3 cut(s) 118, 323, 536
BmcAI AGTACT 2 cut(s) 61, 736
Bme1390I CCNGG 5 cut(s) 409, 614, 830, 879, 1088
Bme18I GGWCC 2 cut(s) 832, 1101
BmeT110I CYCGRG 2 cut(s) 257, 982
BmgT120I GGNCC 7 cut(s) 126, 599, 796, 832, 875, 1084, 1101
BmiI GGNNCC 9 cut(s) 7, 422, 761, 833, 834, 858, 876, 988, 1103
BmrFI CCNGG 5 cut(s) 409, 614, 830, 879, 1088
BmrI ACTGGG 2 cut(s) 652, 1061
BmsI GCATC 1 cut(s) 237
BmtI GCTAGC 1 cut(s) 534
BmuI ACTGGG 2 cut(s) 652, 1061
BplI GAGNNNNNCTC 2 cut(s) 493, 525
Bpu10I CCTNAGC 1 cut(s) 525
BpuMI CCSGG 3 cut(s) 409, 614, 830
BsaHI GRCGYC 1 cut(s) 857
BsaJI CCNNGG 3 cut(s) 49, 424, 878
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 809, 866
Bse118I RCCGGY 5 cut(s) 617, 692, 767, 775, 859
Bse1I ACTGG 2 cut(s) 647, 1056
Bse3DI GCAATG 1 cut(s) 630
BseBI CCWGG 2 cut(s) 879, 1088
BseDI CCNNGG 3 cut(s) 49, 424, 878
BseGI GGATG 3 cut(s) 79, 889, 1015
BseLI CCNNNNNNNGG 3 cut(s) 104, 809, 866
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 3 cut(s) 238, 249, 941
BseNI ACTGG 2 cut(s) 647, 1056
BseRI GAGGAG 1 cut(s) 382
BseX3I CGGCCG 1 cut(s) 615
BseXI GCAGC 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 618
BshFI GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
BshNI GGYRCC 3 cut(s) 5, 856, 986
BsiEI CGRYCG 1 cut(s) 618
BsiHKCI CYCGRG 2 cut(s) 257, 982
BsiSI CCGG 9 cut(s) 409, 452, 614, 618, 693, 768, 776, 829, 860
BslFI GGGAC 1 cut(s) 845
BslI CCNNNNNNNGG 3 cut(s) 104, 809, 866
BsmFI GGGAC 1 cut(s) 845
BsmI GAATGC 1 cut(s) 928
BsnI GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
BsoBI CYCGRG 2 cut(s) 257, 982
Bsp1286I GDGCHC 1 cut(s) 1081
Bsp143I GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
BspACI CCGC 9 cut(s) 210, 277, 290, 321, 324, 534, 561, 597, 863
BspANI GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
BspCNI CTCAG 3 cut(s) 237, 248, 940
BspLI GGNNCC 9 cut(s) 7, 422, 761, 833, 834, 858, 876, 988, 1103
BspOI GCTAGC 1 cut(s) 534
BspPI GGATC 5 cut(s) 200, 754, 767, 1004, 1011
BspT107I GGYRCC 3 cut(s) 5, 856, 986
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 5 cut(s) 617, 692, 767, 775, 859
BsrI ACTGG 2 cut(s) 647, 1056
BssAI RCCGGY 5 cut(s) 617, 692, 767, 775, 859
BssECI CCNNGG 3 cut(s) 49, 424, 878
BssMI GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
BssNI GRCGYC 1 cut(s) 857
BssSI CACGAG 1 cut(s) 676
BssT1I CCWWGG 2 cut(s) 49, 424
Bst2BI CACGAG 1 cut(s) 676
Bst2UI CCWGG 2 cut(s) 879, 1088
Bst4CI ACNGT 3 cut(s) 64, 340, 943
Bst6I CTCTTC 2 cut(s) 510, 809
BstACI GRCGYC 1 cut(s) 857
BstC8I GCNNGC 6 cut(s) 532, 539, 548, 599, 619, 861
BstDEI CTNAG 4 cut(s) 224, 235, 525, 927
BstENI CCTNNNNNAGG 1 cut(s) 102
BstF5I GGATG 3 cut(s) 79, 889, 1015
BstH2I RGCGCY 1 cut(s) 860
BstHHI GCGC 2 cut(s) 623, 859
BstKTI GATC 7 cut(s) 79, 88, 208, 608, 762, 999, 1019
BstMBI GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
BstMCI CGRYCG 1 cut(s) 618
BstMWI GCNNNNNNNGC 3 cut(s) 327, 543, 547
BstNI CCWGG 2 cut(s) 879, 1088
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 5 cut(s) 407, 612, 828, 877, 1086
BstSFI CTRYAG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 759
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 1 cut(s) 759
BstZI CGGCCG 1 cut(s) 615
BsuRI GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
BtsCI GGATG 3 cut(s) 79, 889, 1015
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 2 cut(s) 503, 1049
Cac8I GCNNGC 6 cut(s) 532, 539, 548, 599, 619, 861
CfoI GCGC 2 cut(s) 623, 859
Cfr10I RCCGGY 5 cut(s) 617, 692, 767, 775, 859
Cfr13I GGNCC 7 cut(s) 126, 599, 796, 832, 875, 1084, 1101
CseI GACGC 1 cut(s) 832
Csp6I GTAC 6 cut(s) 6, 60, 735, 773, 987, 1066
CviAII CATG 3 cut(s) 22, 89, 469
CviQI GTAC 6 cut(s) 6, 60, 735, 773, 987, 1066
DdeI CTNAG 4 cut(s) 224, 235, 525, 927
DinI GGCGCC 1 cut(s) 858
DpnI GATC 7 cut(s) 78, 87, 207, 607, 761, 998, 1018
DpnII GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
EaeI YGGCCR 2 cut(s) 615, 777
EagI CGGCCG 1 cut(s) 615
Eam1104I CTCTTC 2 cut(s) 510, 809
EarI CTCTTC 2 cut(s) 510, 809
EciI GGCGGA 1 cut(s) 550
EclXI CGGCCG 1 cut(s) 615
Eco130I CCWWGG 2 cut(s) 49, 424
Eco24I GRGCYC 1 cut(s) 1081
Eco47I GGWCC 2 cut(s) 832, 1101
Eco52I CGGCCG 1 cut(s) 615
Eco88I CYCGRG 2 cut(s) 257, 982
EcoNI CCTNNNNNAGG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 832
EcoRII CCWGG 2 cut(s) 877, 1086
EcoT14I CCWWGG 2 cut(s) 49, 424
EcoT38I GRGCYC 1 cut(s) 1081
EgeI GGCGCC 1 cut(s) 858
EheI GGCGCC 1 cut(s) 858
ErhI CCWWGG 2 cut(s) 49, 424
FaeI CATG 3 cut(s) 25, 92, 472
FaiI YATR 9 cut(s) 23, 90, 158, 470, 490, 610, 714, 808, 1005
FaqI GGGAC 1 cut(s) 845
FatI CATG 3 cut(s) 21, 88, 468
FauI CCCGC 3 cut(s) 217, 297, 590
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 3 cut(s) 117, 322, 535
FokI GGATG 3 cut(s) 66, 896, 1022
FriOI GRGCYC 1 cut(s) 1081
Fsp4HI GCNGC 3 cut(s) 117, 322, 535
FspBI CTAG 6 cut(s) 57, 99, 479, 512, 531, 648
GlaI GCGC 2 cut(s) 622, 858
GluI GCNGC 3 cut(s) 117, 322, 535
HaeII RGCGCY 1 cut(s) 860
HaeIII GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
HapII CCGG 9 cut(s) 409, 452, 614, 618, 693, 768, 776, 829, 860
HgaI GACGC 1 cut(s) 832
HhaI GCGC 2 cut(s) 623, 859
Hin1I GRCGYC 1 cut(s) 857
Hin1II CATG 3 cut(s) 25, 92, 472
Hin6I GCGC 2 cut(s) 621, 857
HinP1I GCGC 2 cut(s) 621, 857
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 3 cut(s) 108, 716, 747
HpaII CCGG 9 cut(s) 409, 452, 614, 618, 693, 768, 776, 829, 860
HphI GGTGA 4 cut(s) 86, 166, 223, 682
Hpy166II GTNNAC 3 cut(s) 433, 466, 773
Hpy188I TCNGA 6 cut(s) 113, 238, 317, 349, 580, 930
Hpy188III TCNNGA 2 cut(s) 83, 512
Hpy8I GTNNAC 3 cut(s) 433, 466, 773
Hpy99I CGWCG 1 cut(s) 1025
HpyAV CCTTC 3 cut(s) 151, 202, 1024
HpyCH4III ACNGT 3 cut(s) 64, 340, 943
HpyCH4IV ACGT 1 cut(s) 459
HpyF10VI GCNNNNNNNGC 3 cut(s) 327, 543, 547
HpyF3I CTNAG 4 cut(s) 224, 235, 525, 927
HpySE526I ACGT 1 cut(s) 459
Hsp92I GRCGYC 1 cut(s) 857
Hsp92II CATG 3 cut(s) 25, 92, 472
HspAI GCGC 2 cut(s) 621, 857
KasI GGCGCC 1 cut(s) 856
KflI GGGWCCC 1 cut(s) 832
KpnI GGTACC 2 cut(s) 9, 990
KroI GCCGGC 2 cut(s) 617, 859
KroNI GCCGGC 2 cut(s) 619, 861
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
LmnI GCTCC 2 cut(s) 279, 831
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 237
MaeI CTAG 6 cut(s) 57, 99, 479, 512, 531, 648
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 5 cut(s) 252, 725, 932, 1047, 1107
MalI GATC 7 cut(s) 78, 87, 207, 607, 761, 998, 1018
MboI GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
MboII GAAGA 2 cut(s) 527, 826
MflI RGATCY 1 cut(s) 759
MhlI GDGCHC 1 cut(s) 1081
MluCI AATT 3 cut(s) 136, 505, 581
Mly113I GGCGCC 1 cut(s) 857
MmeI TCCRAC 1 cut(s) 800
MreI CGCCGGCG 1 cut(s) 859
MroNI GCCGGC 2 cut(s) 617, 859
MseI TTAA 1 cut(s) 554
MspI CCGG 9 cut(s) 409, 452, 614, 618, 693, 768, 776, 829, 860
MspR9I CCNGG 5 cut(s) 409, 614, 830, 879, 1088
Mva1269I GAATGC 1 cut(s) 928
MvaI CCWGG 2 cut(s) 879, 1088
MwoI GCNNNNNNNGC 3 cut(s) 327, 543, 547
NaeI GCCGGC 2 cut(s) 619, 861
NarI GGCGCC 1 cut(s) 857
NciI CCSGG 3 cut(s) 409, 614, 830
NdeII GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
NgoMIV GCCGGC 2 cut(s) 617, 859
NheI GCTAGC 1 cut(s) 530
NlaIII CATG 3 cut(s) 25, 92, 472
NlaIV GGNNCC 9 cut(s) 7, 422, 761, 833, 834, 858, 876, 988, 1103
NmeAIII GCCGAG 1 cut(s) 627
NmuCI GTSAC 2 cut(s) 1047, 1107
NspI RCATGY 1 cut(s) 472
PciI ACATGT 1 cut(s) 468
PctI GAATGC 1 cut(s) 928
PdiI GCCGGC 2 cut(s) 619, 861
PfeI GAWTC 3 cut(s) 108, 716, 747
PflFI GACNNNGTC 1 cut(s) 1105
PfoI TCCNGGA 1 cut(s) 828
PkrI GCNGC 3 cut(s) 118, 323, 536
PluTI GGCGCC 1 cut(s) 860
PpuMI RGGWCCY 1 cut(s) 832
PscI ACATGT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 832
Psp6I CCWGG 2 cut(s) 877, 1086
PspGI CCWGG 2 cut(s) 877, 1086
PspN4I GGNNCC 9 cut(s) 7, 422, 761, 833, 834, 858, 876, 988, 1103
PspPI GGNCC 7 cut(s) 126, 599, 796, 832, 875, 1084, 1101
PspPPI RGGWCCY 1 cut(s) 832
PsuI RGATCY 1 cut(s) 759
PsyI GACNNNGTC 1 cut(s) 1105
RsaI GTAC 6 cut(s) 7, 61, 736, 774, 988, 1067
RsaNI GTAC 6 cut(s) 6, 60, 735, 773, 987, 1066
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 3 cut(s) 117, 322, 535
Sau3AI GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
Sau96I GGNCC 7 cut(s) 126, 599, 796, 832, 875, 1084, 1101
ScaI AGTACT 2 cut(s) 61, 736
ScrFI CCNGG 5 cut(s) 409, 614, 830, 879, 1088
SduI GDGCHC 1 cut(s) 1081
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 1 cut(s) 63
SfoI GGCGCC 1 cut(s) 858
SgrAI CRCCGGYG 2 cut(s) 692, 859
SinI GGWCC 2 cut(s) 832, 1101
SpeI ACTAGT 1 cut(s) 56
Sse9I AATT 3 cut(s) 136, 505, 581
SsiI CCGC 9 cut(s) 210, 277, 290, 321, 324, 534, 561, 597, 863
SspDI GGCGCC 1 cut(s) 856
SspMI CTAG 6 cut(s) 57, 99, 479, 512, 531, 648
StyD4I CCNGG 5 cut(s) 407, 612, 828, 877, 1086
StyI CCWWGG 2 cut(s) 49, 424
TaaI ACNGT 3 cut(s) 64, 340, 943
TaiI ACGT 1 cut(s) 462
TaqI TCGA 1 cut(s) 1015
TasI AATT 3 cut(s) 136, 505, 581
TatI WGTACW 2 cut(s) 59, 734
TauI GCSGC 2 cut(s) 324, 537
TfiI GAWTC 3 cut(s) 108, 716, 747
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 2 cut(s) 503, 1056
TseFI GTSAC 2 cut(s) 1047, 1107
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 2 cut(s) 1047, 1107
TspDTI ATGAA 3 cut(s) 10, 253, 373
TspGWI ACGGA 2 cut(s) 759, 1009
TspRI CASTG 2 cut(s) 503, 1056
Tth111I GACNNNGTC 1 cut(s) 1105
VpaK11BI GGWCC 2 cut(s) 832, 1101
XagI CCTNNNNNAGG 1 cut(s) 102
XapI RAATTY 2 cut(s) 505, 581
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 472
XspI CTAG 6 cut(s) 57, 99, 479, 512, 531, 648
ZrmI AGTACT 2 cut(s) 61, 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.