RchiOBHm_Chr6g0299691

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0014679
Physical Location & Seq
Reverse (-)
60735619 .. 60737124
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26909

Sequence Viewer

Length: 1146 bp
ATGGGTACCCCAACAACATTCATATCATTGTTTCTCATTCTCGTAATCCTTTGCACTAGTACTGTAGCAAATGGTGATCATCCAGATCATGCTAAACCTAGTAAAGGAATCAGAGCAGCCTATTGGCCCTCGTTCAATTCATTCCCTGCTTCCTCTATACAAACCTCTCTTTTCACGCACATTTACTACGCCTTCCTCCTACCCGATCCCGCCACTTTCAAACTCAGCATCACCTCAGACGACCAAACCAAGTTACCCGAGTTCATCGGGTCGCTCCTCAACCTAACCCCGCCCGTGAAAACCCTATTCTCCATCGGAGGCGGCGGTAGCAACGCAACAGTATTCTCGGAGATGGCTAGCACCAAACAAAGCCGTCAAGTATTCATCAATTCCTCCATTGAAGTAGCCCGGAAATACGGGTTCCAAGGTGTTGACTTGGATTGGGAGTTTCCGGCTAACGTGGTGGACATGTCAAACCTAGCTCTGTTATACAAGCAGTGGAGAAATTCTCTAGACGAGGAAGCTAAAGCTTGCGGCAAGCCACGTCTGCTTTTAACCTCCGCCGTGTATTACGCTTCGGAATTTCAAGTTTACGGCGGGCCGAGATCATACCCGGCCGGCGCAATAAGTAAGTACTTGGACTGGGCTAGTCCAATGTGCTTTGACTATCACGGCTCGTGGGAAAACTTCACCGGCGAACATTCGGCCTTGTATGATTCCAAGAGTAACATCAGTACTAACTACGGAATCAGGTCGTGGATCCAAGCCGGTGTACCGGCCAAGAAGCTTGTGATGGGCCTACCTCTATACGGGAAGAGTTGGAGGCTCCGGGACCCGAAAGTGAATGGGGTCGGGGCGGCGGCGGTGGGCGTGGGGCCCGGGGATGATGGTATATTGCTTTATTACCAGATAGTTGAGTTGAATGCTCAAAGTAACGCAACGGTTGTGTTTGACAAGCAAACCAAGTCTTACTATTCCTACTCGGGTACCACTTGGATCGGGTATGACGATGTTCGATCCGTCGCTTTGAAGGTTCGGTTTGCCAAGTCACTGGGCTTGGGTGGGTACTTCTTTTGGGCTCTGGGCCAGGACAAAGATTGGACCCTATCACAGAAAGCTTCAAACGCTTGGAAATATGGCGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

381

Amino Acids

41.93

Weight (kDa)

8.68

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 39 - 364 4.7e-74 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014679)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g40880 FvH4_2g40880
malus_domestica MD08G1059100.v1.1 MD14G1173400.v1.1 MD15G1037300.v1.1
prunus_persica Prupe.1G391300_v2.0.a1
pyrus_communis pycom15g03440
rosa_chinensis RchiOBHm_Chr6g0299691
rosa_laevigata RLG00000011396
rosa_multiflora Rmu_sc0002895.1_g000010
rosa_roxburghii Rroxscaffold_7G00168440
rosa_rugosa Rorug06G0291600
rosa_samantha Rh6AG402400 Rh6BG410900 Rh6CG416700 Rh6DG403800
rosa_wichuraiana Rw6G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 5, 986
AccB1I GGYRCC 2 cut(s) 5, 986
AclWI GGATC 5 cut(s) 200, 754, 767, 1004, 1011
AcoI YGGCCR 2 cut(s) 615, 777
AcsI RAATTY 2 cut(s) 505, 581
AfaI GTAC 7 cut(s) 7, 61, 635, 736, 774, 988, 1067
AfiI CCNNNNNNNGG 2 cut(s) 104, 809
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 7 cut(s) 136, 220, 401, 587, 922, 1030, 1122
AhlI ACTAGT 1 cut(s) 56
AjiI CACGTC 1 cut(s) 545
AjnI CCWGG 1 cut(s) 1086
AluBI AGCT 5 cut(s) 482, 524, 530, 787, 1118
AluI AGCT 5 cut(s) 482, 524, 530, 787, 1118
AlwI GGATC 5 cut(s) 200, 754, 767, 1004, 1011
Ama87I CYCGRG 3 cut(s) 257, 878, 982
AoxI GGCC 8 cut(s) 125, 599, 615, 705, 777, 796, 875, 1084
ApaI GGGCCC 1 cut(s) 879
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 2 cut(s) 505, 581
Asp718I GGTACC 2 cut(s) 5, 986
AspLEI GCGC 1 cut(s) 623
AspS9I GGNCC 8 cut(s) 126, 599, 796, 832, 875, 876, 1084, 1101
AsuC2I CCSGG 5 cut(s) 409, 614, 830, 879, 880
AsuHPI GGTGA 3 cut(s) 86, 223, 682
AsuNHI GCTAGC 1 cut(s) 356
AvaI CYCGRG 3 cut(s) 257, 878, 982
AvaII GGWCC 2 cut(s) 832, 1101
BaeGI GKGCMC 1 cut(s) 879
BaeI ACNNNNGTAYC 2 cut(s) 970, 1003
BamHI GGATCC 1 cut(s) 759
BanI GGYRCC 2 cut(s) 5, 986
BanII GRGCYC 2 cut(s) 879, 1081
BauI CACGAG 1 cut(s) 676
BbvI GCAGC 1 cut(s) 128
BccI CCATC 4 cut(s) 320, 346, 787, 881
BceAI ACGGC 4 cut(s) 357, 548, 610, 688
BciT130I CCWGG 1 cut(s) 1088
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 5 cut(s) 409, 614, 830, 879, 880
BcuI ACTAGT 1 cut(s) 56
BfaI CTAG 6 cut(s) 57, 99, 357, 479, 512, 648
BfmI CTRYAG 1 cut(s) 63
BisI GCNGC 5 cut(s) 117, 322, 535, 858, 861
BlsI GCNGC 5 cut(s) 118, 323, 536, 859, 862
BmcAI AGTACT 3 cut(s) 61, 635, 736
Bme1390I CCNGG 6 cut(s) 409, 614, 830, 879, 880, 1088
Bme18I GGWCC 2 cut(s) 832, 1101
BmeT110I CYCGRG 3 cut(s) 257, 878, 982
BmgBI CACGTC 1 cut(s) 545
BmgT120I GGNCC 8 cut(s) 126, 599, 796, 832, 875, 876, 1084, 1101
BmrFI CCNGG 6 cut(s) 409, 614, 830, 879, 880, 1088
BmrI ACTGGG 2 cut(s) 652, 1061
BmsI GCATC 1 cut(s) 237
BmtI GCTAGC 1 cut(s) 360
BmuI ACTGGG 2 cut(s) 652, 1061
BplI GAGNNNNNCTC 2 cut(s) 493, 525
BpuMI CCSGG 5 cut(s) 409, 614, 830, 879, 880
BsaJI CCNNGG 3 cut(s) 424, 878, 879
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 809
Bse118I RCCGGY 4 cut(s) 617, 692, 767, 775
Bse1I ACTGG 2 cut(s) 647, 1056
BseBI CCWGG 1 cut(s) 1088
BseDI CCNNGG 3 cut(s) 424, 878, 879
BseGI GGATG 2 cut(s) 79, 889
BseLI CCNNNNNNNGG 2 cut(s) 104, 809
BseMII CTCAG 2 cut(s) 238, 249
BseNI ACTGG 2 cut(s) 647, 1056
BseRI GAGGAG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 879
BseX3I CGGCCG 1 cut(s) 615
BseXI GCAGC 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 618
BshFI GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
BshNI GGYRCC 2 cut(s) 5, 986
BsiEI CGRYCG 1 cut(s) 618
BsiHKCI CYCGRG 3 cut(s) 257, 878, 982
BsiSI CCGG 9 cut(s) 409, 452, 614, 618, 693, 768, 776, 829, 879
BslFI GGGAC 1 cut(s) 845
BslI CCNNNNNNNGG 2 cut(s) 104, 809
BsmFI GGGAC 1 cut(s) 845
BsmI GAATGC 1 cut(s) 928
BsnI GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
BsoBI CYCGRG 3 cut(s) 257, 878, 982
Bsp120I GGGCCC 1 cut(s) 875
Bsp1286I GDGCHC 2 cut(s) 879, 1081
Bsp143I GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
BspANI GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
BspCNI CTCAG 2 cut(s) 237, 248
BspOI GCTAGC 1 cut(s) 360
BspPI GGATC 5 cut(s) 200, 754, 767, 1004, 1011
BspT107I GGYRCC 2 cut(s) 5, 986
BsrFI RCCGGY 4 cut(s) 617, 692, 767, 775
BsrI ACTGG 2 cut(s) 647, 1056
BssAI RCCGGY 4 cut(s) 617, 692, 767, 775
BssECI CCNNGG 3 cut(s) 424, 878, 879
BssMI GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
BssSI CACGAG 1 cut(s) 676
BssT1I CCWWGG 1 cut(s) 424
Bst2BI CACGAG 1 cut(s) 676
Bst2UI CCWGG 1 cut(s) 1088
Bst4CI ACNGT 3 cut(s) 64, 340, 943
Bst6I CTCTTC 1 cut(s) 809
BstC8I GCNNGC 5 cut(s) 358, 532, 539, 599, 619
BstDEI CTNAG 2 cut(s) 224, 235
BstENI CCTNNNNNAGG 1 cut(s) 102
BstF5I GGATG 2 cut(s) 79, 889
BstHHI GCGC 1 cut(s) 623
BstKTI GATC 7 cut(s) 79, 88, 208, 608, 762, 999, 1019
BstMBI GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
BstMCI CGRYCG 1 cut(s) 618
BstMWI GCNNNNNNNGC 3 cut(s) 327, 547, 1124
BstNI CCWGG 1 cut(s) 1088
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 6 cut(s) 407, 612, 828, 877, 878, 1086
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 1 cut(s) 879
BstV1I GCAGC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 759
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 1 cut(s) 759
BstZI CGGCCG 1 cut(s) 615
BsuRI GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
BtrI CACGTC 1 cut(s) 545
BtsCI GGATG 2 cut(s) 79, 889
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 2 cut(s) 503, 1049
Cac8I GCNNGC 5 cut(s) 358, 532, 539, 599, 619
CfoI GCGC 1 cut(s) 623
Cfr10I RCCGGY 4 cut(s) 617, 692, 767, 775
Cfr13I GGNCC 8 cut(s) 126, 599, 796, 832, 875, 876, 1084, 1101
Cfr9I CCCGGG 1 cut(s) 878
Csp6I GTAC 7 cut(s) 6, 60, 634, 735, 773, 987, 1066
CviAII CATG 2 cut(s) 89, 469
CviQI GTAC 7 cut(s) 6, 60, 634, 735, 773, 987, 1066
DdeI CTNAG 2 cut(s) 224, 235
DpnI GATC 7 cut(s) 78, 87, 207, 607, 761, 998, 1018
DpnII GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
EaeI YGGCCR 2 cut(s) 615, 777
EagI CGGCCG 1 cut(s) 615
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
EciI GGCGGA 1 cut(s) 550
EclXI CGGCCG 1 cut(s) 615
Eco130I CCWWGG 1 cut(s) 424
Eco24I GRGCYC 2 cut(s) 879, 1081
Eco47I GGWCC 2 cut(s) 832, 1101
Eco52I CGGCCG 1 cut(s) 615
Eco88I CYCGRG 3 cut(s) 257, 878, 982
EcoNI CCTNNNNNAGG 1 cut(s) 102
EcoO109I RGGNCCY 2 cut(s) 832, 875
EcoRII CCWGG 1 cut(s) 1086
EcoT14I CCWWGG 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 879, 1081
ErhI CCWWGG 1 cut(s) 424
FaeI CATG 2 cut(s) 92, 472
FaqI GGGAC 1 cut(s) 845
FatI CATG 2 cut(s) 88, 468
FauI CCCGC 4 cut(s) 217, 297, 590, 1133
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 5 cut(s) 117, 322, 535, 858, 861
FokI GGATG 2 cut(s) 66, 896
FriOI GRGCYC 2 cut(s) 879, 1081
Fsp4HI GCNGC 5 cut(s) 117, 322, 535, 858, 861
FspBI CTAG 6 cut(s) 57, 99, 357, 479, 512, 648
GlaI GCGC 1 cut(s) 622
GluI GCNGC 5 cut(s) 117, 322, 535, 858, 861
HaeIII GGCC 8 cut(s) 127, 601, 617, 707, 779, 798, 877, 1086
HapII CCGG 9 cut(s) 409, 452, 614, 618, 693, 768, 776, 829, 879
HhaI GCGC 1 cut(s) 623
Hin1II CATG 2 cut(s) 92, 472
Hin6I GCGC 1 cut(s) 621
HinP1I GCGC 1 cut(s) 621
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HindIII AAGCTT 3 cut(s) 528, 785, 1116
HinfI GANTC 3 cut(s) 108, 716, 747
HpaII CCGG 9 cut(s) 409, 452, 614, 618, 693, 768, 776, 829, 879
HphI GGTGA 3 cut(s) 86, 223, 682
Hpy166II GTNNAC 4 cut(s) 433, 466, 592, 773
Hpy188I TCNGA 5 cut(s) 113, 238, 317, 349, 580
Hpy188III TCNNGA 2 cut(s) 83, 512
Hpy8I GTNNAC 4 cut(s) 433, 466, 592, 773
Hpy99I CGWCG 1 cut(s) 1025
HpyAV CCTTC 2 cut(s) 202, 1024
HpyCH4III ACNGT 3 cut(s) 64, 340, 943
HpyCH4IV ACGT 2 cut(s) 459, 544
HpyCH4V TGCA 1 cut(s) 54
HpyF10VI GCNNNNNNNGC 3 cut(s) 327, 547, 1124
HpyF3I CTNAG 2 cut(s) 224, 235
HpySE526I ACGT 2 cut(s) 459, 544
Hsp92II CATG 2 cut(s) 92, 472
HspAI GCGC 1 cut(s) 621
KflI GGGWCCC 1 cut(s) 832
KpnI GGTACC 2 cut(s) 9, 990
KroI GCCGGC 1 cut(s) 617
KroNI GCCGGC 1 cut(s) 619
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
LmnI GCTCC 2 cut(s) 279, 831
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 237
MaeI CTAG 6 cut(s) 57, 99, 357, 479, 512, 648
MaeII ACGT 2 cut(s) 459, 544
MaeIII GTNAC 4 cut(s) 252, 725, 932, 1047
MalI GATC 7 cut(s) 78, 87, 207, 607, 761, 998, 1018
MboI GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
MboII GAAGA 1 cut(s) 826
MflI RGATCY 1 cut(s) 759
MhlI GDGCHC 2 cut(s) 879, 1081
MluCI AATT 4 cut(s) 136, 388, 505, 581
MmeI TCCRAC 1 cut(s) 800
MroNI GCCGGC 1 cut(s) 617
MseI TTAA 1 cut(s) 554
MspI CCGG 9 cut(s) 409, 452, 614, 618, 693, 768, 776, 829, 879
MspR9I CCNGG 6 cut(s) 409, 614, 830, 879, 880, 1088
Mva1269I GAATGC 1 cut(s) 928
MvaI CCWGG 1 cut(s) 1088
MwoI GCNNNNNNNGC 3 cut(s) 327, 547, 1124
NaeI GCCGGC 1 cut(s) 619
NciI CCSGG 5 cut(s) 409, 614, 830, 879, 880
NdeII GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
NgoMIV GCCGGC 1 cut(s) 617
NheI GCTAGC 1 cut(s) 356
NlaIII CATG 2 cut(s) 92, 472
NmeAIII GCCGAG 1 cut(s) 627
NmuCI GTSAC 1 cut(s) 1047
NspI RCATGY 1 cut(s) 472
PciI ACATGT 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 1005
PctI GAATGC 1 cut(s) 928
PdiI GCCGGC 1 cut(s) 619
PfeI GAWTC 3 cut(s) 108, 716, 747
PfoI TCCNGGA 1 cut(s) 828
PkrI GCNGC 5 cut(s) 118, 323, 536, 859, 862
PpuMI RGGWCCY 1 cut(s) 832
PscI ACATGT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 832
Psp6I CCWGG 1 cut(s) 1086
PspGI CCWGG 1 cut(s) 1086
PspOMI GGGCCC 1 cut(s) 875
PspPI GGNCC 8 cut(s) 126, 599, 796, 832, 875, 876, 1084, 1101
PspPPI RGGWCCY 1 cut(s) 832
PsuI RGATCY 1 cut(s) 759
RsaI GTAC 7 cut(s) 7, 61, 635, 736, 774, 988, 1067
RsaNI GTAC 7 cut(s) 6, 60, 634, 735, 773, 987, 1066
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 5 cut(s) 117, 322, 535, 858, 861
Sau3AI GATC 7 cut(s) 76, 85, 205, 605, 759, 996, 1016
Sau96I GGNCC 8 cut(s) 126, 599, 796, 832, 875, 876, 1084, 1101
ScaI AGTACT 3 cut(s) 61, 635, 736
ScrFI CCNGG 6 cut(s) 409, 614, 830, 879, 880, 1088
SduI GDGCHC 2 cut(s) 879, 1081
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 1 cut(s) 63
SgrAI CRCCGGYG 1 cut(s) 692
SinI GGWCC 2 cut(s) 832, 1101
SmaI CCCGGG 1 cut(s) 880
SpeI ACTAGT 1 cut(s) 56
Sse9I AATT 4 cut(s) 136, 388, 505, 581
SspMI CTAG 6 cut(s) 57, 99, 357, 479, 512, 648
StyD4I CCNGG 6 cut(s) 407, 612, 828, 877, 878, 1086
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 3 cut(s) 64, 340, 943
TaiI ACGT 2 cut(s) 462, 547
TaqI TCGA 1 cut(s) 1015
TasI AATT 4 cut(s) 136, 388, 505, 581
TatI WGTACW 3 cut(s) 59, 633, 734
TauI GCSGC 4 cut(s) 324, 537, 860, 863
TfiI GAWTC 3 cut(s) 108, 716, 747
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 2 cut(s) 503, 1056
TseFI GTSAC 1 cut(s) 1047
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 1047
TspDTI ATGAA 4 cut(s) 10, 129, 253, 373
TspGWI ACGGA 2 cut(s) 759, 1009
TspMI CCCGGG 1 cut(s) 878
TspRI CASTG 2 cut(s) 503, 1056
VpaK11BI GGWCC 2 cut(s) 832, 1101
XagI CCTNNNNNAGG 1 cut(s) 102
XapI RAATTY 2 cut(s) 505, 581
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 472
XmaI CCCGGG 1 cut(s) 878
XspI CTAG 6 cut(s) 57, 99, 357, 479, 512, 648
ZrmI AGTACT 3 cut(s) 61, 635, 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.