MD15G1037300.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014679
Physical Location & Seq
Forward (+)
2592053 .. 2593919
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1037300.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGCCACCCCAAAGTTGATGCCTTTTTTCATCCTTGTTGTCTTCCACACAATGCTTTCTTCGAGCTCGGGTTCGAATTCGGATCTTTTTTATCCATCTGCAAAAGGTATCAGAGCAGCCTATTTCCCATCATTTAACTCGTTCTCGCCATCCTCTATAGACACCCAATACTTCACCCACATCTACTATGCTTTCCTCGTACCCGAACCCTCCACTTACAAACTCAACGTCACCGAATTCGACCAAGCCAGAATACCCGAATTCATCCGAGCGCTACGGACCAAAAAGCCGGCCGTCACAACCCTCCTGTCTATAGGAGGCGGCGGTAGCAATTCAACCACGTTTGCACTCATGGCTTCCACCCAGGCAACCCGAGAAGCTTTCATCCGCTCCACAATTCAGGTGGCCCGGAAATATGGGTTCCAAGGCCTTGACTTGGACTGGGAGTTTCCAGAAGATGCTGTGGAAATGTCAAATCTTGGCTTGTTGTTCGAGCAATGGAGAAAGGCCCTGGATAATGAGGCTAGAGTTACCGGTAAACCCCGTCTGCTTTTGACCGCCGCAGTATATTATGCTTGGAAATTTACCGTTTATGGCGGTCCAAGATCTTATCCGGCCGCGGCAATAACTAAATATTTGGATTGGGCCAGCCCAATGTGCTTTGATTATCATGGGTCGTGGGATGATTTCACCGGGATGAATGCTGCGTTGTATGATTCCAAGAGTAATATTAGTACCCATTATGGAATCGGGTCGTGGATCGAAGCCGGTGTCCCGCCCAAAAAGCTCGTCATGGGTCTGCCTTTGTACGGGAAGACTTGGACGCTCAAGGACCCGAAAGTGAACGGAATCGGGGCACCGGCTTCGGGTGTTGGGCCGGGGGATGGGATTTTGGTTTACCGCCAAATAGTTGATTTTAACAGCAGAACAAATGCGACCGTCGTGTTTGATGTCGAATCAGCGTCTTATTATTCTTTATCCGGTAGCACTTGGGTCGGGTATGATGGTGTCCGGTCCGCGAACGTGAAGGTTCGGTTCGCTAAGTCTTTGGGCTTGGGCGGATACTTCTTTTGGGCTCTGGGCCAGGACAATGGATGGGACCTATCTAAACAAGCTTCTAATGCTTGGAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

379

Amino Acids

41.81

Weight (kDa)

8.96

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 40 - 363 4.4e-69 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014679)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g40880 FvH4_2g40880
malus_domestica MD08G1059100.v1.1 MD14G1173400.v1.1 MD15G1037300.v1.1
prunus_persica Prupe.1G391300_v2.0.a1
pyrus_communis pycom15g03440
rosa_chinensis RchiOBHm_Chr6g0299691
rosa_laevigata RLG00000011396
rosa_multiflora Rmu_sc0002895.1_g000010
rosa_roxburghii Rroxscaffold_7G00168440
rosa_rugosa Rorug06G0291600
rosa_samantha Rh6AG402400 Rh6BG410900 Rh6CG416700 Rh6DG403800
rosa_wichuraiana Rw6G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 856
AccBSI CCGCTC 1 cut(s) 390
AccII CGCG 2 cut(s) 620, 1019
AclWI GGATC 2 cut(s) 90, 767
AcoI YGGCCR 3 cut(s) 3, 291, 615
AcsI RAATTY 4 cut(s) 76, 236, 260, 581
AfaI GTAC 3 cut(s) 201, 736, 809
AfeI AGCGCT 1 cut(s) 273
AfiI CCNNNNNNNGG 4 cut(s) 436, 809, 866, 884
AgeI ACCGGT 1 cut(s) 533
AgsI TTSAA 1 cut(s) 336
AjnI CCWGG 3 cut(s) 363, 510, 1083
AluBI AGCT 4 cut(s) 66, 380, 787, 1115
AluI AGCT 4 cut(s) 66, 380, 787, 1115
Alw21I GWGCWC 1 cut(s) 68
AlwI GGATC 2 cut(s) 90, 767
Ama87I CYCGRG 2 cut(s) 67, 372
Aor51HI AGCGCT 1 cut(s) 273
AoxI GGCC 9 cut(s) 3, 291, 405, 427, 507, 615, 645, 875, 1081
ApeKI GCWGC 2 cut(s) 116, 704
ApoI RAATTY 4 cut(s) 76, 236, 260, 581
ArsI GACNNNNNNTTYG 6 cut(s) 213, 245, 756, 774, 788, 806
AsiGI ACCGGT 1 cut(s) 533
AspLEI GCGC 1 cut(s) 274
AsuC2I CCSGG 3 cut(s) 409, 694, 879
AsuHPI GGTGA 3 cut(s) 166, 223, 682
AsuII TTCGAA 1 cut(s) 74
AvaI CYCGRG 2 cut(s) 67, 372
AvaII GGWCC 5 cut(s) 279, 599, 832, 1014, 1099
BaeGI GKGCMC 1 cut(s) 859
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 856
BanII GRGCYC 2 cut(s) 68, 1078
BbsI GAAGAC 2 cut(s) 34, 821
Bbv12I GWGCWC 1 cut(s) 68
BbvI GCAGC 2 cut(s) 128, 691
BccI CCATC 6 cut(s) 103, 136, 157, 878, 998, 1089
BceAI ACGGC 1 cut(s) 278
BcgI CGANNNNNNTGC 4 cut(s) 846, 880, 976, 1010
BciT130I CCWGG 3 cut(s) 365, 512, 1085
BciVI GTATCC 1 cut(s) 1055
BcnI CCSGG 3 cut(s) 409, 694, 879
BfaI CTAG 1 cut(s) 525
BfmI CTRYAG 2 cut(s) 156, 312
BfoI RGCGCY 1 cut(s) 275
BfuI GTATCC 1 cut(s) 1055
BglII AGATCT 1 cut(s) 605
BisI GCNGC 6 cut(s) 117, 322, 561, 618, 621, 705
BlsI GCNGC 6 cut(s) 118, 323, 562, 619, 622, 706
Bme1390I CCNGG 6 cut(s) 365, 409, 512, 694, 879, 1085
Bme18I GGWCC 5 cut(s) 279, 599, 832, 1014, 1099
BmeT110I CYCGRG 2 cut(s) 67, 372
BmiI GGNNCC 4 cut(s) 422, 834, 858, 1100
BmrFI CCNGG 6 cut(s) 365, 409, 512, 694, 879, 1085
BmrI ACTGGG 1 cut(s) 451
BmsI GCATC 2 cut(s) 9, 448
BmuI ACTGGG 1 cut(s) 451
BpiI GAAGAC 2 cut(s) 34, 821
Bpu14I TTCGAA 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 812
BpuMI CCSGG 3 cut(s) 409, 694, 879
BsaJI CCNNGG 5 cut(s) 363, 424, 510, 618, 878
BsaWI WCCGGW 3 cut(s) 533, 980, 1011
Bsc4I CCNNNNNNNGG 4 cut(s) 436, 809, 866, 884
Bse118I RCCGGY 4 cut(s) 289, 533, 767, 859
Bse1I ACTGG 1 cut(s) 446
Bse3DI GCAATG 1 cut(s) 503
BseBI CCWGG 3 cut(s) 365, 512, 1085
BseDI CCNNGG 5 cut(s) 363, 424, 510, 618, 878
BseGI GGATG 8 cut(s) 30, 149, 264, 384, 688, 702, 889, 1100
BseLI CCNNNNNNNGG 4 cut(s) 436, 809, 866, 884
BseMI GCAATG 1 cut(s) 503
BseNI ACTGG 1 cut(s) 446
BseSI GKGCMC 1 cut(s) 859
BseX3I CGGCCG 2 cut(s) 291, 615
BseXI GCAGC 2 cut(s) 128, 691
Bsh1236I CGCG 2 cut(s) 620, 1019
Bsh1285I CGRYCG 3 cut(s) 294, 618, 939
BshFI GGCC 9 cut(s) 5, 293, 407, 429, 509, 617, 647, 877, 1083
BshNI GGYRCC 1 cut(s) 856
BshTI ACCGGT 1 cut(s) 533
BsiEI CGRYCG 3 cut(s) 294, 618, 939
BsiHKAI GWGCWC 1 cut(s) 68
BsiHKCI CYCGRG 2 cut(s) 67, 372
BslFI GGGAC 2 cut(s) 758, 1112
BslI CCNNNNNNNGG 4 cut(s) 436, 809, 866, 884
BsmFI GGGAC 2 cut(s) 758, 1112
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 9 cut(s) 5, 293, 407, 429, 509, 617, 647, 877, 1083
BsoBI CYCGRG 2 cut(s) 67, 372
Bsp119I TTCGAA 1 cut(s) 74
Bsp1286I GDGCHC 3 cut(s) 68, 859, 1078
Bsp143I GATC 3 cut(s) 82, 605, 759
BspANI GGCC 9 cut(s) 5, 293, 407, 429, 509, 617, 647, 877, 1083
BspFNI CGCG 2 cut(s) 620, 1019
BspLI GGNNCC 4 cut(s) 422, 834, 858, 1100
BspPI GGATC 2 cut(s) 90, 767
BspT104I TTCGAA 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 856
BsrBI CCGCTC 1 cut(s) 390
BsrDI GCAATG 1 cut(s) 503
BsrFI RCCGGY 4 cut(s) 289, 533, 767, 859
BsrI ACTGG 1 cut(s) 446
BssAI RCCGGY 4 cut(s) 289, 533, 767, 859
BssECI CCNNGG 5 cut(s) 363, 424, 510, 618, 878
BssMI GATC 3 cut(s) 82, 605, 759
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 3 cut(s) 365, 512, 1085
Bst4CI ACNGT 2 cut(s) 589, 940
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 291, 649
BstDEI CTNAG 1 cut(s) 1041
BstDSI CCRYGG 1 cut(s) 618
BstF5I GGATG 8 cut(s) 30, 149, 264, 384, 688, 702, 889, 1100
BstFNI CGCG 2 cut(s) 620, 1019
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 3 cut(s) 85, 608, 762
BstMBI GATC 3 cut(s) 82, 605, 759
BstMCI CGRYCG 3 cut(s) 294, 618, 939
BstMWI GCNNNNNNNGC 5 cut(s) 327, 353, 657, 784, 1121
BstNI CCWGG 3 cut(s) 365, 512, 1085
BstSCI CCNGG 6 cut(s) 363, 407, 510, 692, 877, 1083
BstSFI CTRYAG 2 cut(s) 156, 312
BstSLI GKGCMC 1 cut(s) 859
BstUI CGCG 2 cut(s) 620, 1019
BstV1I GCAGC 2 cut(s) 128, 691
BstV2I GAAGAC 2 cut(s) 34, 821
BstX2I RGATCY 2 cut(s) 82, 605
BstXI CCANNNNNNTGG 1 cut(s) 1091
BstYI RGATCY 2 cut(s) 82, 605
BstZI CGGCCG 2 cut(s) 291, 615
BsuI GTATCC 1 cut(s) 1055
BsuRI GGCC 9 cut(s) 5, 293, 407, 429, 509, 617, 647, 877, 1083
BtgI CCRYGG 1 cut(s) 618
BtsCI GGATG 8 cut(s) 30, 149, 264, 384, 688, 702, 889, 1100
Cac8I GCNNGC 2 cut(s) 291, 649
CfoI GCGC 1 cut(s) 274
Cfr10I RCCGGY 4 cut(s) 289, 533, 767, 859
Cfr42I CCGCGG 1 cut(s) 621
CpoI CGGWCCG 1 cut(s) 1014
CseI GACGC 2 cut(s) 832, 951
Csp6I GTAC 3 cut(s) 200, 735, 808
CspAI ACCGGT 1 cut(s) 533
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CspI CGGWCCG 1 cut(s) 1014
CviAII CATG 3 cut(s) 352, 671, 793
CviQI GTAC 3 cut(s) 200, 735, 808
DdeI CTNAG 1 cut(s) 1041
DpnI GATC 3 cut(s) 84, 607, 761
DpnII GATC 3 cut(s) 82, 605, 759
EaeI YGGCCR 3 cut(s) 3, 291, 615
EagI CGGCCG 2 cut(s) 291, 615
EciI GGCGGA 1 cut(s) 1074
Ecl136II GAGCTC 1 cut(s) 66
EclXI CGGCCG 2 cut(s) 291, 615
Eco130I CCWWGG 1 cut(s) 424
Eco147I AGGCCT 1 cut(s) 429
Eco24I GRGCYC 2 cut(s) 68, 1078
Eco47I GGWCC 5 cut(s) 279, 599, 832, 1014, 1099
Eco47III AGCGCT 1 cut(s) 273
Eco52I CGGCCG 2 cut(s) 291, 615
Eco53kI GAGCTC 1 cut(s) 66
Eco88I CYCGRG 2 cut(s) 67, 372
EcoICRI GAGCTC 1 cut(s) 66
EcoO109I RGGNCCY 3 cut(s) 508, 832, 1099
EcoRI GAATTC 3 cut(s) 76, 236, 260
EcoRII CCWGG 3 cut(s) 363, 510, 1083
EcoT14I CCWWGG 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 68, 1078
ErhI CCWWGG 1 cut(s) 424
FaeI CATG 3 cut(s) 355, 674, 796
FaqI GGGAC 2 cut(s) 758, 1112
FatI CATG 3 cut(s) 351, 670, 792
FauI CCCGC 1 cut(s) 783
Fnu4HI GCNGC 6 cut(s) 117, 322, 561, 618, 621, 705
FokI GGATG 8 cut(s) 17, 136, 251, 371, 695, 709, 896, 1107
FriOI GRGCYC 2 cut(s) 68, 1078
Fsp4HI GCNGC 6 cut(s) 117, 322, 561, 618, 621, 705
FspBI CTAG 1 cut(s) 525
GlaI GCGC 1 cut(s) 273
GluI GCNGC 6 cut(s) 117, 322, 561, 618, 621, 705
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 9 cut(s) 5, 293, 407, 429, 509, 617, 647, 877, 1083
HgaI GACGC 2 cut(s) 832, 951
HhaI GCGC 1 cut(s) 274
Hin1II CATG 3 cut(s) 355, 674, 796
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HindIII AAGCTT 2 cut(s) 378, 1113
HinfI GANTC 4 cut(s) 716, 747, 849, 956
HphI GGTGA 3 cut(s) 166, 223, 682
Hpy166II GTNNAC 3 cut(s) 539, 844, 898
Hpy188I TCNGA 3 cut(s) 82, 113, 269
Hpy188III TCNNGA 1 cut(s) 452
Hpy8I GTNNAC 3 cut(s) 539, 844, 898
Hpy99I CGWCG 1 cut(s) 944
HpyAV CCTTC 1 cut(s) 1021
HpyCH4III ACNGT 2 cut(s) 589, 940
HpyCH4IV ACGT 3 cut(s) 228, 341, 1023
HpyCH4V TGCA 2 cut(s) 101, 347
HpyF10VI GCNNNNNNNGC 5 cut(s) 327, 353, 657, 784, 1121
HpyF3I CTNAG 1 cut(s) 1041
HpySE526I ACGT 3 cut(s) 228, 341, 1023
Hsp92II CATG 3 cut(s) 355, 674, 796
HspAI GCGC 1 cut(s) 272
KroI GCCGGC 1 cut(s) 289
KroNI GCCGGC 1 cut(s) 291
KspI CCGCGG 1 cut(s) 621
Kzo9I GATC 3 cut(s) 82, 605, 759
LmnI GCTCC 1 cut(s) 395
Lsp1109I GCAGC 2 cut(s) 128, 691
LweI GCATC 2 cut(s) 9, 448
MaeI CTAG 1 cut(s) 525
MaeII ACGT 3 cut(s) 228, 341, 1023
MaeIII GTNAC 3 cut(s) 229, 295, 529
MalI GATC 3 cut(s) 84, 607, 761
MbiI CCGCTC 1 cut(s) 390
MboI GATC 3 cut(s) 82, 605, 759
MboII GAAGA 4 cut(s) 34, 51, 467, 826
MflI RGATCY 2 cut(s) 82, 605
MhlI GDGCHC 3 cut(s) 68, 859, 1078
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 76, 236, 260, 331, 396, 581
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 6 cut(s) 163, 206, 220, 311, 314, 514
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 289
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 135, 918
MslI CAYNNNNRTG 1 cut(s) 695
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 620
MspR9I CCNGG 6 cut(s) 365, 409, 512, 694, 879, 1085
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 3 cut(s) 365, 512, 1085
MvnI CGCG 2 cut(s) 620, 1019
MwoI GCNNNNNNNGC 5 cut(s) 327, 353, 657, 784, 1121
NaeI GCCGGC 1 cut(s) 291
NciI CCSGG 3 cut(s) 409, 694, 879
NdeII GATC 3 cut(s) 82, 605, 759
NgoMIV GCCGGC 1 cut(s) 289
NlaIII CATG 3 cut(s) 355, 674, 796
NlaIV GGNNCC 4 cut(s) 422, 834, 858, 1100
NmuCI GTSAC 2 cut(s) 229, 295
NspV TTCGAA 1 cut(s) 74
PceI AGGCCT 1 cut(s) 429
PctI GAATGC 1 cut(s) 706
PdiI GCCGGC 1 cut(s) 291
PfeI GAWTC 4 cut(s) 716, 747, 849, 956
PinAI ACCGGT 1 cut(s) 533
PkrI GCNGC 6 cut(s) 118, 323, 562, 619, 622, 706
PpuMI RGGWCCY 2 cut(s) 832, 1099
Psp124BI GAGCTC 1 cut(s) 68
Psp5II RGGWCCY 2 cut(s) 832, 1099
Psp6I CCWGG 3 cut(s) 363, 510, 1083
PspGI CCWGG 3 cut(s) 363, 510, 1083
PspN4I GGNNCC 4 cut(s) 422, 834, 858, 1100
PspPPI RGGWCCY 2 cut(s) 832, 1099
PsuI RGATCY 2 cut(s) 82, 605
RsaI GTAC 3 cut(s) 201, 736, 809
RsaNI GTAC 3 cut(s) 200, 735, 808
RseI CAYNNNNRTG 1 cut(s) 695
Rsr2I CGGWCCG 1 cut(s) 1014
RsrII CGGWCCG 1 cut(s) 1014
SacI GAGCTC 1 cut(s) 68
SacII CCGCGG 1 cut(s) 621
SaqAI TTAA 2 cut(s) 135, 918
SatI GCNGC 6 cut(s) 117, 322, 561, 618, 621, 705
Sau3AI GATC 3 cut(s) 82, 605, 759
ScrFI CCNGG 6 cut(s) 365, 409, 512, 694, 879, 1085
SduI GDGCHC 3 cut(s) 68, 859, 1078
SfaNI GCATC 2 cut(s) 9, 448
SfcI CTRYAG 2 cut(s) 156, 312
Sfr303I CCGCGG 1 cut(s) 621
SfuI TTCGAA 1 cut(s) 74
SgrBI CCGCGG 1 cut(s) 621
SinI GGWCC 5 cut(s) 279, 599, 832, 1014, 1099
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
Sse9I AATT 6 cut(s) 76, 236, 260, 331, 396, 581
SseBI AGGCCT 1 cut(s) 429
SspI AATATT 2 cut(s) 635, 730
SspMI CTAG 1 cut(s) 525
SstI GAGCTC 1 cut(s) 68
StuI AGGCCT 1 cut(s) 429
StyD4I CCNGG 6 cut(s) 363, 407, 510, 692, 877, 1083
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 2 cut(s) 589, 940
TaiI ACGT 3 cut(s) 231, 344, 1026
TaqI TCGA 6 cut(s) 62, 74, 240, 492, 762, 954
TasI AATT 6 cut(s) 76, 236, 260, 331, 396, 581
TauI GCSGC 4 cut(s) 324, 563, 620, 623
TfiI GAWTC 4 cut(s) 716, 747, 849, 956
Tru1I TTAA 2 cut(s) 135, 918
Tru9I TTAA 2 cut(s) 135, 918
TseFI GTSAC 2 cut(s) 229, 295
TseI GCWGC 2 cut(s) 116, 704
Tsp45I GTSAC 2 cut(s) 229, 295
TspDTI ATGAA 4 cut(s) 19, 253, 373, 713
TspGWI ACGGA 2 cut(s) 292, 861
VpaK11BI GGWCC 5 cut(s) 279, 599, 832, 1014, 1099
XapI RAATTY 4 cut(s) 76, 236, 260, 581
XcmI CCANNNNNNNNNTGG 1 cut(s) 400
XspI CTAG 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.