MD14G1173400.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014679
Physical Location & Seq
Forward (+)
26730043 .. 26730783
741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1173400.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGCTTCCACGCAGGCGACCAGAGAAGTTTTCATCAACTCCACAATTCAGGTGGCTCGGAAATACGGGTTCTACGGCGTTGACTTGGTCTGGGTATTTCCTAAGGATGCTGCGGAAATGTCAAATCTTGCCTTGTTATACAAGGAATGGAGAAAGGCTCTAAATAATGAGCCTAGAGTTACCGGGAAGCCCCGTTTACTTTTAACCTCCGCGGTATATTATGCATCGAAATTTACTTTTTACGACGGTCCGAGATCGTATCCGGCCGGGGCAATAAGTAAATATTTGGATTGGGCCAGCCCAATGTGCTATGATTATCATGGGTCATGGGAAAATTTCACCGGGATGAATGCTGCGTTGGATGATTCCAACAGTAATATTAGTACTCGTTACGGAATCGGGTCGTGGATTGAAGCCGGTGTCCCACCCAAAAAGCTGGTCGTGGGTCTGCCTTTGTACGGGCGGACTTGGACGCTCAAGGACTCGAAAGTGAACGGGATCGGGGCACCGGCTTTGGGTGTGGGCTCTTGGGATGGTGTTTTGGTCTATCACCAAATCGTTCATTTTAACAAGAGAACCAATGCAACAGTTGTGTTTGATACCGAATCAGCGTCTTACTATTCTTATTCGGGTAGCTCTTGGATCGGGTATGATGGTGTCCGGTCCGTGAACGTGAAGGTCCGGTGCGAAGTCGTTGGGCTTGGGCGGATACTTCTCTTGGGCTCTGGGCCAGGACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

247

Amino Acids

27.16

Weight (kDa)

9.16

Isoelectric Point (pI)

27.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 1 - 227 1.3e-46 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014679)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g40880 FvH4_2g40880
malus_domestica MD08G1059100.v1.1 MD14G1173400.v1.1 MD15G1037300.v1.1
prunus_persica Prupe.1G391300_v2.0.a1
pyrus_communis pycom15g03440
rosa_chinensis RchiOBHm_Chr6g0299691
rosa_laevigata RLG00000011396
rosa_multiflora Rmu_sc0002895.1_g000010
rosa_roxburghii Rroxscaffold_7G00168440
rosa_rugosa Rorug06G0291600
rosa_samantha Rh6AG402400 Rh6BG410900 Rh6CG416700 Rh6DG403800
rosa_wichuraiana Rw6G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 505
AccII CGCG 1 cut(s) 212
AciI CCGC 5 cut(s) 113, 210, 212, 463, 706
AclWI GGATC 2 cut(s) 506, 650
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 2 cut(s) 230, 334
AfaI GTAC 2 cut(s) 385, 458
AfiI CCNNNNNNNGG 2 cut(s) 458, 515
AgsI TTSAA 1 cut(s) 413
AjnI CCWGG 1 cut(s) 730
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AluBI AGCT 2 cut(s) 436, 636
AluI AGCT 2 cut(s) 436, 636
AlwI GGATC 2 cut(s) 506, 650
AoxI GGCC 3 cut(s) 264, 294, 728
ApeKI GCWGC 2 cut(s) 110, 353
ApoI RAATTY 2 cut(s) 230, 334
ArsI GACNNNNNNTTYG 2 cut(s) 423, 455
AspS9I GGNCC 5 cut(s) 248, 294, 663, 679, 728
AsuC2I CCSGG 3 cut(s) 184, 268, 343
AsuHPI GGTGA 2 cut(s) 331, 542
AvaII GGWCC 3 cut(s) 248, 663, 679
AxyI CCTNAGG 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 508
BanI GGYRCC 1 cut(s) 505
BanII GRGCYC 2 cut(s) 527, 725
BbvI GCAGC 2 cut(s) 97, 340
BccI CCATC 2 cut(s) 527, 647
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 732
BciVI GTATCC 2 cut(s) 270, 702
BcnI CCSGG 3 cut(s) 184, 268, 343
BfaI CTAG 1 cut(s) 174
BfuI GTATCC 2 cut(s) 270, 702
BisI GCNGC 2 cut(s) 111, 354
BlsI GCNGC 2 cut(s) 112, 355
BmcAI AGTACT 1 cut(s) 385
Bme1390I CCNGG 4 cut(s) 184, 268, 343, 732
Bme18I GGWCC 3 cut(s) 248, 663, 679
BmgT120I GGNCC 5 cut(s) 248, 294, 663, 679, 728
BmiI GGNNCC 1 cut(s) 507
BmrFI CCNGG 4 cut(s) 184, 268, 343, 732
BmsI GCATC 2 cut(s) 97, 233
BplI GAGNNNNNCTC 2 cut(s) 142, 174
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 3 cut(s) 184, 268, 343
BsaJI CCNNGG 2 cut(s) 210, 267
BsaWI WCCGGW 2 cut(s) 660, 681
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 515
Bse118I RCCGGY 2 cut(s) 416, 508
Bse21I CCTNAGG 1 cut(s) 102
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 2 cut(s) 210, 267
BseGI GGATG 4 cut(s) 112, 351, 367, 538
BseLI CCNNNNNNNGG 2 cut(s) 458, 515
BseSI GKGCMC 1 cut(s) 508
BseX3I CGGCCG 1 cut(s) 264
BseXI GCAGC 2 cut(s) 97, 340
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 3 cut(s) 266, 296, 730
BshNI GGYRCC 1 cut(s) 505
BsiEI CGRYCG 1 cut(s) 267
BsiSI CCGG 8 cut(s) 183, 263, 267, 342, 417, 509, 661, 682
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 2 cut(s) 458, 515
BsmFI GGGAC 1 cut(s) 407
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 3 cut(s) 266, 296, 730
Bsp1286I GDGCHC 3 cut(s) 508, 527, 725
Bsp143I GATC 3 cut(s) 254, 498, 642
BspACI CCGC 5 cut(s) 113, 210, 212, 463, 706
BspANI GGCC 3 cut(s) 266, 296, 730
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 1 cut(s) 507
BspPI GGATC 2 cut(s) 506, 650
BspT107I GGYRCC 1 cut(s) 505
BsrFI RCCGGY 2 cut(s) 416, 508
BssAI RCCGGY 2 cut(s) 416, 508
BssECI CCNNGG 2 cut(s) 210, 267
BssMI GATC 3 cut(s) 254, 498, 642
Bst2UI CCWGG 1 cut(s) 732
Bst4CI ACNGT 3 cut(s) 248, 374, 589
BstC8I GCNNGC 2 cut(s) 15, 298
BstDEI CTNAG 1 cut(s) 102
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 4 cut(s) 112, 351, 367, 538
BstFNI CGCG 1 cut(s) 212
BstKTI GATC 3 cut(s) 257, 501, 645
BstMBI GATC 3 cut(s) 254, 498, 642
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 1 cut(s) 732
BstSCI CCNGG 4 cut(s) 182, 266, 341, 730
BstSLI GKGCMC 1 cut(s) 508
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 97, 340
BstXI CCANNNNNNTGG 1 cut(s) 436
BstZI CGGCCG 1 cut(s) 264
Bsu36I CCTNAGG 1 cut(s) 102
BsuI GTATCC 2 cut(s) 270, 702
BsuRI GGCC 3 cut(s) 266, 296, 730
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 4 cut(s) 112, 351, 367, 538
Cac8I GCNNGC 2 cut(s) 15, 298
Cfr10I RCCGGY 2 cut(s) 416, 508
Cfr13I GGNCC 5 cut(s) 248, 294, 663, 679, 728
Cfr42I CCGCGG 1 cut(s) 213
CpoI CGGWCCG 2 cut(s) 248, 663
CseI GACGC 2 cut(s) 481, 600
Csp6I GTAC 2 cut(s) 384, 457
CspCI CAANNNNNGTGG 2 cut(s) 33, 68
CspI CGGWCCG 2 cut(s) 248, 663
CviAII CATG 2 cut(s) 320, 327
CviQI GTAC 2 cut(s) 384, 457
DdeI CTNAG 1 cut(s) 102
DpnI GATC 3 cut(s) 256, 500, 644
DpnII GATC 3 cut(s) 254, 498, 642
EaeI YGGCCR 1 cut(s) 264
EagI CGGCCG 1 cut(s) 264
EciI GGCGGA 2 cut(s) 478, 721
EclXI CGGCCG 1 cut(s) 264
Eco24I GRGCYC 2 cut(s) 527, 725
Eco47I GGWCC 3 cut(s) 248, 663, 679
Eco52I CGGCCG 1 cut(s) 264
Eco81I CCTNAGG 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 730
EcoT22I ATGCAT 1 cut(s) 226
EcoT38I GRGCYC 2 cut(s) 527, 725
FaeI CATG 2 cut(s) 323, 330
FaiI YATR 7 cut(s) 139, 217, 222, 312, 321, 328, 651
FaqI GGGAC 1 cut(s) 407
FatI CATG 2 cut(s) 319, 326
Fnu4HI GCNGC 2 cut(s) 111, 354
FokI GGATG 4 cut(s) 119, 358, 374, 545
FriOI GRGCYC 2 cut(s) 527, 725
Fsp4HI GCNGC 2 cut(s) 111, 354
FspBI CTAG 1 cut(s) 174
GluI GCNGC 2 cut(s) 111, 354
HaeIII GGCC 3 cut(s) 266, 296, 730
HapII CCGG 8 cut(s) 183, 263, 267, 342, 417, 509, 661, 682
HgaI GACGC 2 cut(s) 481, 600
Hin1II CATG 2 cut(s) 323, 330
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 4 cut(s) 365, 396, 482, 605
HpaII CCGG 8 cut(s) 183, 263, 267, 342, 417, 509, 661, 682
HphI GGTGA 2 cut(s) 331, 542
Hpy166II GTNNAC 4 cut(s) 82, 197, 493, 670
Hpy188I TCNGA 2 cut(s) 60, 252
Hpy8I GTNNAC 4 cut(s) 82, 197, 493, 670
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 1 cut(s) 670
HpyCH4III ACNGT 3 cut(s) 248, 374, 589
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 2 cut(s) 224, 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 1 cut(s) 672
Hsp92II CATG 2 cut(s) 323, 330
KspI CCGCGG 1 cut(s) 213
Kzo9I GATC 3 cut(s) 254, 498, 642
Lsp1109I GCAGC 2 cut(s) 97, 340
LweI GCATC 2 cut(s) 97, 233
MaeI CTAG 1 cut(s) 174
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 2 cut(s) 178, 389
MalI GATC 3 cut(s) 256, 500, 644
MboI GATC 3 cut(s) 254, 498, 642
MhlI GDGCHC 3 cut(s) 508, 527, 725
MluCI AATT 3 cut(s) 45, 230, 334
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 2 cut(s) 339, 393
MnlI CCTC 1 cut(s) 217
Mph1103I ATGCAT 1 cut(s) 226
MseI TTAA 2 cut(s) 203, 567
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 212
MspI CCGG 8 cut(s) 183, 263, 267, 342, 417, 509, 661, 682
MspR9I CCNGG 4 cut(s) 184, 268, 343, 732
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 306
NciI CCSGG 3 cut(s) 184, 268, 343
NdeII GATC 3 cut(s) 254, 498, 642
NlaIII CATG 2 cut(s) 323, 330
NlaIV GGNNCC 1 cut(s) 507
NsiI ATGCAT 1 cut(s) 226
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 3 cut(s) 365, 396, 605
PflFI GACNNNGTC 1 cut(s) 86
PkrI GCNGC 2 cut(s) 112, 355
PleI GAGTC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 730
PspGI CCWGG 1 cut(s) 730
PspN4I GGNNCC 1 cut(s) 507
PspPI GGNCC 5 cut(s) 248, 294, 663, 679, 728
PsyI GACNNNGTC 1 cut(s) 86
RsaI GTAC 2 cut(s) 385, 458
RsaNI GTAC 2 cut(s) 384, 457
RseI CAYNNNNRTG 1 cut(s) 344
Rsr2I CGGWCCG 2 cut(s) 248, 663
RsrII CGGWCCG 2 cut(s) 248, 663
SacII CCGCGG 1 cut(s) 213
SaqAI TTAA 2 cut(s) 203, 567
SatI GCNGC 2 cut(s) 111, 354
Sau3AI GATC 3 cut(s) 254, 498, 642
Sau96I GGNCC 5 cut(s) 248, 294, 663, 679, 728
ScaI AGTACT 1 cut(s) 385
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 4 cut(s) 184, 268, 343, 732
SduI GDGCHC 3 cut(s) 508, 527, 725
SetI ASST 6 cut(s) 54, 209, 438, 638, 675, 681
SfaNI GCATC 2 cut(s) 97, 233
Sfr303I CCGCGG 1 cut(s) 213
SgrBI CCGCGG 1 cut(s) 213
SinI GGWCC 3 cut(s) 248, 663, 679
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 3 cut(s) 45, 230, 334
SsiI CCGC 5 cut(s) 113, 210, 212, 463, 706
SspI AATATT 2 cut(s) 284, 379
SspMI CTAG 1 cut(s) 174
StyD4I CCNGG 4 cut(s) 182, 266, 341, 730
TaaI ACNGT 3 cut(s) 248, 374, 589
TaiI ACGT 1 cut(s) 675
TaqI TCGA 2 cut(s) 227, 485
TasI AATT 3 cut(s) 45, 230, 334
TatI WGTACW 1 cut(s) 383
TfiI GAWTC 3 cut(s) 365, 396, 605
Tru1I TTAA 2 cut(s) 203, 567
Tru9I TTAA 2 cut(s) 203, 567
TseI GCWGC 2 cut(s) 110, 353
TspDTI ATGAA 3 cut(s) 22, 362, 551
TspGWI ACGGA 2 cut(s) 408, 655
Tth111I GACNNNGTC 1 cut(s) 86
VpaK11BI GGWCC 3 cut(s) 248, 663, 679
XapI RAATTY 2 cut(s) 230, 334
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XspI CTAG 1 cut(s) 174
ZrmI AGTACT 1 cut(s) 385
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.