MD08G1059100.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014679
Physical Location & Seq
Reverse (-)
4714277 .. 4715778
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1059100.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGGTACCCCAAAGTTGATGCCTTTTTTCATCCTTGTAATCTTCCACACAATGCTTTCTTACGGCTCGGGTTCGAATTCGGATCATATTTATCCATCTGGGAAAGGAATCAGAGCAGCCTATTTTCCATCATCTAATTCGTTCTCACCATCCTCTATAGACACCCAATACTTCACCCACATCTACTATGCTTTCCTCCAACCCGAATCCACCACTTACAAACTCAACGTCACCGAATTCGACCAAGCGAGAATACCCGAATTCATCAAAGCGCTCCGGAGCAAAAACCCGCCTGTCACAACACTCCTGTCCATGGGAGGCGGCGGTAACAATGCAACCGTGTTTGCACTCATGGTTTCCACCCAGGCGACCCGGGAAGTTTTCATCAACTCAACAATTCAGGTGGCACGGAAATATGGCTTCCAAGGGGTTGACTTGGATTGGGAGTTTCCTAAAGATGCCCTGGAAATGTCAAATCTTGCCTTGTTGTACAAGGAATGGAGAAAGGCTCTGGATAATGAGGCTAGAGTTTGCGGTAAACCCCGTTTACTTTTAACCTCCGCGGTATATTACGCATCGAAATTTACTTTTTACGGCGGTCCGAGATCGTATCCGGCCGGGGCAATAAGTAAATATTTGGATTGGGCCAGCCCAATGTGCTTTGATTATCATGGGTCATGGGAAAATTTCACCGGGATGAATGCTGCGTTGGATGATTCCAAGAGTAATATTAGTACTCATTATGGAATCGGGTCATGGATCGAAGCCGGTGTCCCGTCCAAAAAGCTCGTCATGGGTCTGCCTTTGTACGGGCGGACTTGGACGCTCAAGGACTCGAAAGTGAACGGAATCGGGGCACCGGCTTTGGGTATGGGCCCTGGGGATGGTGTTTTGGTTTATCACCAAATACTTCATTTTAACAACAGAACCAACGCAACTGTTGTGTATGATACCGAATCAGCGTCTTACTATTCTTATTCGGGTAGCTCTTGGGTCGGGTATGATGGTGTCCGGTCCGCGAACATGAAGGTCCGGTTCGCCAAGTCGTTGGGCTTGGGCGGATACTTTTTTTGGGCTCTGGGCCAGGACAATGAATGGGCCATATCAAAACAAGCTTCTAATGCTTGGAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

379

Amino Acids

41.96

Weight (kDa)

8.98

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 40 - 363 3.1e-70 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014679)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g40880 FvH4_2g40880
malus_domestica MD08G1059100.v1.1 MD14G1173400.v1.1 MD15G1037300.v1.1
prunus_persica Prupe.1G391300_v2.0.a1
pyrus_communis pycom15g03440
rosa_chinensis RchiOBHm_Chr6g0299691
rosa_laevigata RLG00000011396
rosa_multiflora Rmu_sc0002895.1_g000010
rosa_roxburghii Rroxscaffold_7G00168440
rosa_rugosa Rorug06G0291600
rosa_samantha Rh6AG402400 Rh6BG410900 Rh6CG416700 Rh6DG403800
rosa_wichuraiana Rw6G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 2 cut(s) 5, 856
AccII CGCG 2 cut(s) 563, 1019
AccIII TCCGGA 1 cut(s) 276
AclWI GGATC 2 cut(s) 90, 767
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 5 cut(s) 76, 236, 260, 581, 685
AfaI GTAC 4 cut(s) 7, 491, 736, 809
AfeI AGCGCT 1 cut(s) 273
AfiI CCNNNNNNNGG 4 cut(s) 313, 809, 866, 884
AjnI CCWGG 4 cut(s) 363, 462, 877, 1083
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AluBI AGCT 3 cut(s) 787, 987, 1115
AluI AGCT 3 cut(s) 787, 987, 1115
AlwI GGATC 2 cut(s) 90, 767
Ama87I CYCGRG 2 cut(s) 67, 372
Aor13HI TCCGGA 1 cut(s) 276
Aor51HI AGCGCT 1 cut(s) 273
AoxI GGCC 5 cut(s) 615, 645, 874, 1081, 1098
ApaI GGGCCC 1 cut(s) 878
ApeKI GCWGC 2 cut(s) 116, 704
ApoI RAATTY 5 cut(s) 76, 236, 260, 581, 685
ArsI GACNNNNNNTTYG 6 cut(s) 213, 245, 756, 774, 788, 806
Asp718I GGTACC 1 cut(s) 5
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 8 cut(s) 599, 645, 874, 875, 1014, 1030, 1081, 1098
AsuC2I CCSGG 4 cut(s) 373, 374, 619, 694
AsuHPI GGTGA 5 cut(s) 138, 166, 223, 682, 893
AsuII TTCGAA 1 cut(s) 74
AvaI CYCGRG 2 cut(s) 67, 372
AvaII GGWCC 3 cut(s) 599, 1014, 1030
BaeGI GKGCMC 2 cut(s) 859, 878
BanI GGYRCC 2 cut(s) 5, 856
BanII GRGCYC 2 cut(s) 878, 1078
BbvI GCAGC 2 cut(s) 128, 691
BccI CCATC 5 cut(s) 103, 136, 157, 878, 998
BceAI ACGGC 2 cut(s) 79, 610
BciT130I CCWGG 4 cut(s) 365, 464, 879, 1085
BciVI GTATCC 2 cut(s) 621, 1055
BcnI CCSGG 4 cut(s) 373, 374, 619, 694
BfaI CTAG 1 cut(s) 525
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 275
BfuI GTATCC 2 cut(s) 621, 1055
BisI GCNGC 3 cut(s) 117, 322, 705
BlsI GCNGC 3 cut(s) 118, 323, 706
BmcAI AGTACT 1 cut(s) 736
Bme1390I CCNGG 8 cut(s) 365, 373, 374, 464, 619, 694, 879, 1085
Bme18I GGWCC 3 cut(s) 599, 1014, 1030
BmeT110I CYCGRG 2 cut(s) 67, 372
BmgT120I GGNCC 8 cut(s) 599, 645, 874, 875, 1014, 1030, 1081, 1098
BmiI GGNNCC 3 cut(s) 7, 858, 876
BmrFI CCNGG 8 cut(s) 365, 373, 374, 464, 619, 694, 879, 1085
BmsI GCATC 3 cut(s) 9, 448, 584
BplI GAGNNNNNCTC 2 cut(s) 493, 525
Bpu14I TTCGAA 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 812
BpuMI CCSGG 4 cut(s) 373, 374, 619, 694
BsaJI CCNNGG 9 cut(s) 312, 363, 372, 424, 462, 561, 618, 877, 878
BsaWI WCCGGW 3 cut(s) 276, 1011, 1032
Bsc4I CCNNNNNNNGG 4 cut(s) 313, 809, 866, 884
Bse118I RCCGGY 2 cut(s) 767, 859
BseAI TCCGGA 1 cut(s) 276
BseBI CCWGG 4 cut(s) 365, 464, 879, 1085
BseDI CCNNGG 9 cut(s) 312, 363, 372, 424, 462, 561, 618, 877, 878
BseGI GGATG 5 cut(s) 30, 149, 702, 718, 889
BseLI CCNNNNNNNGG 4 cut(s) 313, 809, 866, 884
BseSI GKGCMC 2 cut(s) 859, 878
BseX3I CGGCCG 1 cut(s) 615
BseXI GCAGC 2 cut(s) 128, 691
Bsh1236I CGCG 2 cut(s) 563, 1019
Bsh1285I CGRYCG 1 cut(s) 618
BshFI GGCC 5 cut(s) 617, 647, 876, 1083, 1100
BshNI GGYRCC 2 cut(s) 5, 856
BsiEI CGRYCG 1 cut(s) 618
BsiHKCI CYCGRG 2 cut(s) 67, 372
BsiSI CCGG 9 cut(s) 277, 373, 614, 618, 693, 768, 860, 1012, 1033
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 4 cut(s) 313, 809, 866, 884
BsmFI GGGAC 1 cut(s) 758
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 5 cut(s) 617, 647, 876, 1083, 1100
BsoBI CYCGRG 2 cut(s) 67, 372
Bsp119I TTCGAA 1 cut(s) 74
Bsp120I GGGCCC 1 cut(s) 874
Bsp1286I GDGCHC 3 cut(s) 859, 878, 1078
Bsp13I TCCGGA 1 cut(s) 276
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 3 cut(s) 82, 605, 759
Bsp19I CCATGG 1 cut(s) 312
BspANI GGCC 5 cut(s) 617, 647, 876, 1083, 1100
BspEI TCCGGA 1 cut(s) 276
BspFNI CGCG 2 cut(s) 563, 1019
BspLI GGNNCC 3 cut(s) 7, 858, 876
BspPI GGATC 2 cut(s) 90, 767
BspT104I TTCGAA 1 cut(s) 74
BspT107I GGYRCC 2 cut(s) 5, 856
BsrFI RCCGGY 2 cut(s) 767, 859
BsrGI TGTACA 1 cut(s) 489
BssAI RCCGGY 2 cut(s) 767, 859
BssECI CCNNGG 9 cut(s) 312, 363, 372, 424, 462, 561, 618, 877, 878
BssMI GATC 3 cut(s) 82, 605, 759
BssT1I CCWWGG 2 cut(s) 312, 424
Bst2UI CCWGG 4 cut(s) 365, 464, 879, 1085
Bst4CI ACNGT 2 cut(s) 340, 940
BstAUI TGTACA 1 cut(s) 489
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 649
BstDSI CCRYGG 2 cut(s) 312, 561
BstF5I GGATG 5 cut(s) 30, 149, 702, 718, 889
BstFNI CGCG 2 cut(s) 563, 1019
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 3 cut(s) 85, 608, 762
BstMBI GATC 3 cut(s) 82, 605, 759
BstMCI CGRYCG 1 cut(s) 618
BstMWI GCNNNNNNNGC 2 cut(s) 657, 1121
BstNI CCWGG 4 cut(s) 365, 464, 879, 1085
BstSCI CCNGG 8 cut(s) 363, 371, 372, 462, 617, 692, 877, 1083
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 2 cut(s) 859, 878
BstUI CGCG 2 cut(s) 563, 1019
BstV1I GCAGC 2 cut(s) 128, 691
BstXI CCANNNNNNTGG 1 cut(s) 1048
BstZI CGGCCG 1 cut(s) 615
BsuI GTATCC 2 cut(s) 621, 1055
BsuRI GGCC 5 cut(s) 617, 647, 876, 1083, 1100
BtgI CCRYGG 2 cut(s) 312, 561
BtsCI GGATG 5 cut(s) 30, 149, 702, 718, 889
Cac8I GCNNGC 1 cut(s) 649
CfoI GCGC 1 cut(s) 274
Cfr10I RCCGGY 2 cut(s) 767, 859
Cfr13I GGNCC 8 cut(s) 599, 645, 874, 875, 1014, 1030, 1081, 1098
Cfr42I CCGCGG 1 cut(s) 564
Cfr9I CCCGGG 1 cut(s) 372
CpoI CGGWCCG 2 cut(s) 599, 1014
CseI GACGC 2 cut(s) 832, 951
Csp6I GTAC 4 cut(s) 6, 490, 735, 808
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CspI CGGWCCG 2 cut(s) 599, 1014
CviAII CATG 7 cut(s) 313, 352, 671, 678, 756, 793, 1024
CviQI GTAC 4 cut(s) 6, 490, 735, 808
DpnI GATC 3 cut(s) 84, 607, 761
DpnII GATC 3 cut(s) 82, 605, 759
EaeI YGGCCR 1 cut(s) 615
EagI CGGCCG 1 cut(s) 615
EciI GGCGGA 2 cut(s) 829, 1074
EclXI CGGCCG 1 cut(s) 615
Eco130I CCWWGG 2 cut(s) 312, 424
Eco24I GRGCYC 2 cut(s) 878, 1078
Eco47I GGWCC 3 cut(s) 599, 1014, 1030
Eco47III AGCGCT 1 cut(s) 273
Eco52I CGGCCG 1 cut(s) 615
Eco88I CYCGRG 2 cut(s) 67, 372
EcoO109I RGGNCCY 1 cut(s) 875
EcoRI GAATTC 3 cut(s) 76, 236, 260
EcoRII CCWGG 4 cut(s) 363, 462, 877, 1083
EcoT14I CCWWGG 2 cut(s) 312, 424
EcoT38I GRGCYC 2 cut(s) 878, 1078
ErhI CCWWGG 2 cut(s) 312, 424
FaeI CATG 7 cut(s) 316, 355, 674, 681, 759, 796, 1027
FaqI GGGAC 1 cut(s) 758
FatI CATG 7 cut(s) 312, 351, 670, 677, 755, 792, 1023
FauI CCCGC 1 cut(s) 297
Fnu4HI GCNGC 3 cut(s) 117, 322, 705
FokI GGATG 5 cut(s) 17, 136, 709, 725, 896
FriOI GRGCYC 2 cut(s) 878, 1078
Fsp4HI GCNGC 3 cut(s) 117, 322, 705
FspBI CTAG 1 cut(s) 525
GlaI GCGC 1 cut(s) 273
GluI GCNGC 3 cut(s) 117, 322, 705
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 5 cut(s) 617, 647, 876, 1083, 1100
HapII CCGG 9 cut(s) 277, 373, 614, 618, 693, 768, 860, 1012, 1033
HgaI GACGC 2 cut(s) 832, 951
HhaI GCGC 1 cut(s) 274
Hin1II CATG 7 cut(s) 316, 355, 674, 681, 759, 796, 1027
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 1113
HinfI GANTC 7 cut(s) 108, 206, 716, 747, 833, 849, 956
HpaII CCGG 9 cut(s) 277, 373, 614, 618, 693, 768, 860, 1012, 1033
HphI GGTGA 5 cut(s) 138, 166, 223, 682, 893
Hpy166II GTNNAC 4 cut(s) 433, 539, 548, 844
Hpy188I TCNGA 3 cut(s) 82, 113, 603
Hpy188III TCNNGA 2 cut(s) 277, 512
Hpy8I GTNNAC 4 cut(s) 433, 539, 548, 844
HpyAV CCTTC 1 cut(s) 1021
HpyCH4III ACNGT 2 cut(s) 340, 940
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 2 cut(s) 335, 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 657, 1121
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 7 cut(s) 316, 355, 674, 681, 759, 796, 1027
HspAI GCGC 1 cut(s) 272
Kpn2I TCCGGA 1 cut(s) 276
KpnI GGTACC 1 cut(s) 9
KspI CCGCGG 1 cut(s) 564
Kzo9I GATC 3 cut(s) 82, 605, 759
LmnI GCTCC 2 cut(s) 279, 279
Lsp1109I GCAGC 2 cut(s) 128, 691
LweI GCATC 3 cut(s) 9, 448, 584
MaeI CTAG 1 cut(s) 525
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 3 cut(s) 229, 295, 326
MalI GATC 3 cut(s) 84, 607, 761
MboI GATC 3 cut(s) 82, 605, 759
MboII GAAGA 1 cut(s) 34
MhlI GDGCHC 3 cut(s) 859, 878, 1078
MluCI AATT 7 cut(s) 76, 136, 236, 260, 396, 581, 685
MlyI GAGTC 1 cut(s) 827
MmeI TCCRAC 2 cut(s) 223, 690
MnlI CCTC 5 cut(s) 163, 206, 311, 514, 568
MroI TCCGGA 1 cut(s) 276
MseI TTAA 2 cut(s) 554, 918
MslI CAYNNNNRTG 1 cut(s) 695
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 9 cut(s) 277, 373, 614, 618, 693, 768, 860, 1012, 1033
MspR9I CCNGG 8 cut(s) 365, 373, 374, 464, 619, 694, 879, 1085
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 4 cut(s) 365, 464, 879, 1085
MvnI CGCG 2 cut(s) 563, 1019
MwoI GCNNNNNNNGC 2 cut(s) 657, 1121
NciI CCSGG 4 cut(s) 373, 374, 619, 694
NcoI CCATGG 1 cut(s) 312
NdeII GATC 3 cut(s) 82, 605, 759
NlaIII CATG 7 cut(s) 316, 355, 674, 681, 759, 796, 1027
NlaIV GGNNCC 3 cut(s) 7, 858, 876
NmuCI GTSAC 2 cut(s) 229, 295
NspV TTCGAA 1 cut(s) 74
PasI CCCWGGG 1 cut(s) 878
PctI GAATGC 1 cut(s) 706
PfeI GAWTC 6 cut(s) 108, 206, 716, 747, 849, 956
PkrI GCNGC 3 cut(s) 118, 323, 706
PleI GAGTC 1 cut(s) 827
PpsI GAGTC 1 cut(s) 827
Psp6I CCWGG 4 cut(s) 363, 462, 877, 1083
PspGI CCWGG 4 cut(s) 363, 462, 877, 1083
PspN4I GGNNCC 3 cut(s) 7, 858, 876
PspOMI GGGCCC 1 cut(s) 874
PspPI GGNCC 8 cut(s) 599, 645, 874, 875, 1014, 1030, 1081, 1098
RsaI GTAC 4 cut(s) 7, 491, 736, 809
RsaNI GTAC 4 cut(s) 6, 490, 735, 808
RseI CAYNNNNRTG 1 cut(s) 695
Rsr2I CGGWCCG 2 cut(s) 599, 1014
RsrII CGGWCCG 2 cut(s) 599, 1014
SacII CCGCGG 1 cut(s) 564
SaqAI TTAA 2 cut(s) 554, 918
SatI GCNGC 3 cut(s) 117, 322, 705
Sau3AI GATC 3 cut(s) 82, 605, 759
Sau96I GGNCC 8 cut(s) 599, 645, 874, 875, 1014, 1030, 1081, 1098
ScaI AGTACT 1 cut(s) 736
SchI GAGTC 1 cut(s) 827
ScrFI CCNGG 8 cut(s) 365, 373, 374, 464, 619, 694, 879, 1085
SduI GDGCHC 3 cut(s) 859, 878, 1078
SetI ASST 7 cut(s) 231, 405, 560, 789, 989, 1032, 1117
SfaNI GCATC 3 cut(s) 9, 448, 584
SfcI CTRYAG 1 cut(s) 156
Sfr303I CCGCGG 1 cut(s) 564
SfuI TTCGAA 1 cut(s) 74
SgrBI CCGCGG 1 cut(s) 564
SinI GGWCC 3 cut(s) 599, 1014, 1030
SmaI CCCGGG 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
Sse9I AATT 7 cut(s) 76, 136, 236, 260, 396, 581, 685
SspI AATATT 2 cut(s) 635, 730
SspMI CTAG 1 cut(s) 525
StyD4I CCNGG 8 cut(s) 363, 371, 372, 462, 617, 692, 877, 1083
StyI CCWWGG 2 cut(s) 312, 424
TaaI ACNGT 2 cut(s) 340, 940
TaiI ACGT 1 cut(s) 231
TaqI TCGA 5 cut(s) 74, 240, 578, 762, 836
TasI AATT 7 cut(s) 76, 136, 236, 260, 396, 581, 685
TatI WGTACW 2 cut(s) 489, 734
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 6 cut(s) 108, 206, 716, 747, 849, 956
Tru1I TTAA 2 cut(s) 554, 918
Tru9I TTAA 2 cut(s) 554, 918
TseFI GTSAC 2 cut(s) 229, 295
TseI GCWGC 2 cut(s) 116, 704
Tsp45I GTSAC 2 cut(s) 229, 295
TspDTI ATGAA 7 cut(s) 19, 253, 373, 713, 902, 1040, 1107
TspGWI ACGGA 2 cut(s) 424, 861
TspMI CCCGGG 1 cut(s) 372
VpaK11BI GGWCC 3 cut(s) 599, 1014, 1030
XapI RAATTY 5 cut(s) 76, 236, 260, 581, 685
XmaI CCCGGG 1 cut(s) 372
XspI CTAG 1 cut(s) 525
ZrmI AGTACT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.