Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0012724

Physical Location & Seq

Genomic Coordinates Forward (+)

881776 .. 885746

Gene Structure 3971 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g01710.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGGAACCTGCTTGCAAGGGGTTGTGTTGTCTCTGTTTCTCTCGAGCTTGTTGTTGTCGACTCTGGTGTCGTCTGCACCGAATGACGGGTTGGTTAGGATTGGACTGAAGAAGGTGAAACCGGATGCGAATCAGCGGCTTGCTGCAGGTTACAAGAATGGAGAGGGTTCGATTAGAAAGTATCGTTTCGGTTCTAATGTGGGTGACGCTGAGGGGGAGGATATTGATATTGTCAAATTGAAGAACTACTTGGATGCTCAGTACTATGGGGAAATTGCTATTGGCACTCCTCCTCAGCCGTTCACTGTGATTTTTGACACTGGCAGCTCTAATCTGTGGGTTCCTTCCAACAAGTGTTATTTTTCGGTTGCGTGCTACTTCCATGCCAAGTACAAGTCGAGCGATTCGAGTACATACAAGAAGAATGGGAAATCTGCTGCCATTCAGTATGGTACTGGATCTATTTCAGGGTTCTTTAGCAATGACAATGTTAAAGTTGGGGACCTCGTCGTGAAGGATCAGGAATTTATTGAAGCAACTAGTGAGCCTGGCTTAACATTCCTGGCTGCTAAGTTTGATGGGTTATTAGGCCTTGGATTTCAAGAGATTTCGGTTGGAAATGCTGTCCCAGTGTGGTACAACATGATTAAGCAAGGGCTTATCAAGGAACCAGTGTTTTCTTTCTGGATGAACCGCAAAGTGGCAGCACAAGAAGGTGGTGAACTTGTGTTTGGAGGAGTTGATCCCAATCACTTCAAGGGAAAGCACACTTATGTTCCTGTAACAAAGAAAGGCTATTGGCAGTTCGACATGGGTGATGTTCTTATTGGTGATAAACCAACTGGATATTGTGCTGGTGGTTGTAATGCAATCGCAGATTCTGGAACTTCTCTTTTGGCTGGTCCAACGACAGTGGTTGCCATGATAAATAAAGCCATTGGAGCCAATGGGGTTGTTAGCCAGGAATGCAAGGCAGTTGTTCAACAGTATGGTCAAACCATTCTGGAACTCCTCTTGGCTGAGGCCCAACCAAGGAAGATCTGCTCCCAAATTGGAGTGTGCACCTTTGATGGGACTCGTAGTGTTAGTATGGGCATTGAGAGTGTGGTGGATAAGAACCGTAAAAAATCTTCTGGAGTTCTTCATGATGCTTCATGCTCTGCTTGTGAGATGGCAGTTGTCTGGATGCAAAACCAGCTTCAGCAGAATCAGACCCAGGAATTTATTTTGAACTATGTCAATGAGCTTTGTGATCGAATGCCAAGCCCCAATGGACAATCGGCTGTTGACTGCCAACGTCTTTCTACCATGCCTACCATTTCCTTCACCATTGGTGGTAAAGTTTTTCCGCTCCGTGCAGAGGAGTATGTACTCAAAGTGGGCGAGGGTGCTCAGGCCCAGTGCATAAGTGGTTTCATTGGTATGGACGTTCCTCCTCCCAGGGGACCTCTCTGGATCCTAGGAGACGTCTTCATGGGTCGCTACCACACTGTCTTTGATTATGGTAAAAAGAGAGTTGGATTTGCTGAGGCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0010035 GO:0010038 GO:0016787 GO:0019538 GO:0030163 GO:0042221 GO:0043170 GO:0044238 GO:0046686 GO:0050896 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

513

Amino Acids

55.33

Weight (kDa)

7.43

Isoelectric Point (pI)

31.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	87 - 511	1.4e-117	Eukaryotic aspartyl protease
TAXi_N	PF14543	88 - 245	1.4e-11	Xylanase inhibitor N-terminal
SapB_2	PF03489	321 - 354	3.2e-11	Saposin-like type B, region 2
SapB_1	PF05184	385 - 421	4.7e-13	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04460 AT4G04460
fragaria_vesca	FvH4_3g01710
malus_domestica	MD05G1349700.v1.1 MD10G1324400.v1.1
prunus_persica	Prupe.4G017700_v2.0.a1 Prupe.4G017700_v2.0.a1
pyrus_communis	pycom05g31730 pycom10g27480
rosa_chinensis	RchiOBHm_Chr5g0002571
rosa_laevigata	RLG00000031040
rosa_roxburghii	Rroxscaffold_1G00073340
rosa_rugosa	Rorug04G0397000
rosa_samantha	Rh5AG020400 Rh5BG023100 Rh5CG022400 Rh5DG021500
rosa_wichuraiana	Rw5G001880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	67
AatII	GACGTC	1 cut(s)	1470
Acc36I	ACCTGC	2 cut(s)	17, 137
AccBSI	CCGCTC	1 cut(s)	1351
AccI	GTMKAC	1 cut(s)	59
AciI	CCGC	3 cut(s)	136, 694, 1349
AclWI	GGATC	5 cut(s)	466, 525, 737, 1450, 1463
AcsI	RAATTY	2 cut(s)	524, 1220
AcuI	CTGAAG	2 cut(s)	128, 1184
AcyI	GRCGYC	1 cut(s)	1467
AfaI	GTAC	6 cut(s)	263, 392, 412, 454, 638, 1371
AfiI	CCNNNNNNNGG	6 cut(s)	86, 700, 1032, 1071, 1360, 1442
AgsI	TTSAA	6 cut(s)	241, 533, 602, 757, 983, 1231
AhlI	ACTAGT	1 cut(s)	539
AjnI	CCWGG	5 cut(s)	547, 561, 960, 1215, 1439
AjuI	GAANNNNNNNTTGG	8 cut(s)	74, 106, 233, 264, 265, 296, 714, 746
AluBI	AGCT	4 cut(s)	48, 327, 1198, 1246
AluI	AGCT	4 cut(s)	48, 327, 1198, 1246
Alw21I	GWGCWC	2 cut(s)	1064, 1393
Alw26I	GTCTC	2 cut(s)	36, 1458
Alw44I	GTGCAC	1 cut(s)	1060
AlwI	GGATC	5 cut(s)	466, 525, 737, 1450, 1463
AlwNI	CAGNNNCTG	1 cut(s)	881
Ama87I	CYCGRG	1 cut(s)	43
AoxI	GGCC	3 cut(s)	589, 1023, 1395
ApaLI	GTGCAC	1 cut(s)	1060
ApeKI	GCWGC	6 cut(s)	143, 324, 437, 566, 704, 1532
ApoI	RAATTY	2 cut(s)	524, 1220
AspA2I	CCTAGG	1 cut(s)	1459
AspS9I	GGNCC	5 cut(s)	502, 902, 1024, 1396, 1445
AsuHPI	GGTGA	6 cut(s)	127, 215, 731, 827, 842, 1318
AvaI	CYCGRG	1 cut(s)	43
AvaII	GGWCC	3 cut(s)	502, 902, 1445
AvrII	CCTAGG	1 cut(s)	1459
BaeGI	GKGCMC	1 cut(s)	1064
BamHI	GGATCC	1 cut(s)	1455
BarI	GAAGNNNNNNTAC	2 cut(s)	1114, 1146
BbsI	GAAGAC	1 cut(s)	1462
Bbv12I	GWGCWC	2 cut(s)	1064, 1393
BbvCI	CCTCAGC	4 cut(s)	210, 294, 1020, 1527
BbvI	GCAGC	6 cut(s)	130, 336, 424, 553, 716, 1519
BccI	CCATC	3 cut(s)	572, 1064, 1165
BceAI	ACGGC	1 cut(s)	283
BciT130I	CCWGG	5 cut(s)	549, 563, 962, 1217, 1441
BcoDI	GTCTC	2 cut(s)	36, 1458
BcuI	ACTAGT	1 cut(s)	539
BfaI	CTAG	2 cut(s)	540, 1460
BfmI	CTRYAG	1 cut(s)	144
BfuAI	ACCTGC	2 cut(s)	17, 137
BglII	AGATCT	1 cut(s)	1038
BisI	GCNGC	7 cut(s)	137, 144, 325, 438, 567, 705, 1533
BlnI	CCTAGG	1 cut(s)	1459
BlsI	GCNGC	7 cut(s)	138, 145, 326, 439, 568, 706, 1534
BmcAI	AGTACT	1 cut(s)	263
Bme1390I	CCNGG	5 cut(s)	549, 563, 962, 1217, 1441
Bme18I	GGWCC	3 cut(s)	502, 902, 1445
BmeT110I	CYCGRG	1 cut(s)	43
BmgT120I	GGNCC	5 cut(s)	502, 902, 1024, 1396, 1445
BmiI	GGNNCC	7 cut(s)	7, 342, 503, 669, 943, 1446, 1457
BmrFI	CCNGG	5 cut(s)	549, 563, 962, 1217, 1441
BmrI	ACTGGG	2 cut(s)	623, 1393
BmsI	GCATC	4 cut(s)	115, 244, 1138, 1176
BmuI	ACTGGG	2 cut(s)	623, 1393
BpiI	GAAGAC	1 cut(s)	1462
BpmI	CTGGAG	1 cut(s)	1155
Bpu10I	CCTNAGC	5 cut(s)	210, 294, 1020, 1392, 1527
BsaBI	GATNNNNATC	2 cut(s)	129, 747
BsaHI	GRCGYC	1 cut(s)	1467
BsaJI	CCNNGG	6 cut(s)	592, 1031, 1215, 1439, 1440, 1459
BsaWI	WCCGGW	1 cut(s)	121
Bsc4I	CCNNNNNNNGG	6 cut(s)	86, 700, 1032, 1071, 1360, 1442
Bse1I	ACTGG	6 cut(s)	325, 460, 629, 671, 847, 1399
Bse3DI	GCAATG	1 cut(s)	487
Bse8I	GATNNNNATC	2 cut(s)	129, 747
BseBI	CCWGG	5 cut(s)	549, 563, 962, 1217, 1441
BseDI	CCNNGG	6 cut(s)	592, 1031, 1215, 1439, 1440, 1459
BseGI	GGATG	4 cut(s)	130, 259, 693, 1191
BseJI	GATNNNNATC	2 cut(s)	129, 747
BseLI	CCNNNNNNNGG	6 cut(s)	86, 700, 1032, 1071, 1360, 1442
BseMI	GCAATG	1 cut(s)	487
BseMII	CTCAG	6 cut(s)	201, 272, 308, 1011, 1406, 1518
BseNI	ACTGG	6 cut(s)	325, 460, 629, 671, 847, 1399
BseRI	GAGGAG	6 cut(s)	279, 282, 750, 1001, 1376, 1425
BseSI	GKGCMC	1 cut(s)	1064
BseXI	GCAGC	6 cut(s)	130, 336, 424, 553, 716, 1519
BsgI	GTGCAG	2 cut(s)	60, 1377
BshFI	GGCC	3 cut(s)	591, 1025, 1397
BsiHKAI	GWGCWC	2 cut(s)	1064, 1393
BsiHKCI	CYCGRG	1 cut(s)	43
BsiSI	CCGG	1 cut(s)	122
BslFI	GGGAC	4 cut(s)	515, 611, 1087, 1458
BslI	CCNNNNNNNGG	6 cut(s)	86, 700, 1032, 1071, 1360, 1442
BsmAI	GTCTC	2 cut(s)	36, 1458
BsmBI	CGTCTC	1 cut(s)	1458
BsmFI	GGGAC	4 cut(s)	515, 611, 1087, 1458
BsmI	GAATGC	2 cut(s)	971, 1263
BsnI	GGCC	3 cut(s)	591, 1025, 1397
BsoBI	CYCGRG	1 cut(s)	43
Bsp1286I	GDGCHC	2 cut(s)	1064, 1393
Bsp143I	GATC	6 cut(s)	458, 517, 742, 1038, 1252, 1455
BspACI	CCGC	3 cut(s)	136, 694, 1349
BspANI	GGCC	3 cut(s)	591, 1025, 1397
BspCNI	CTCAG	6 cut(s)	202, 271, 307, 1012, 1405, 1519
BspHI	TCATGA	1 cut(s)	1144
BspLI	GGNNCC	7 cut(s)	7, 342, 503, 669, 943, 1446, 1457
BspMAI	CTGCAG	1 cut(s)	148
BspMI	ACCTGC	2 cut(s)	17, 137
BspPI	GGATC	5 cut(s)	466, 525, 737, 1450, 1463
BsrBI	CCGCTC	1 cut(s)	1351
BsrDI	GCAATG	1 cut(s)	487
BsrI	ACTGG	6 cut(s)	325, 460, 629, 671, 847, 1399
BssECI	CCNNGG	6 cut(s)	592, 1031, 1215, 1439, 1440, 1459
BssMI	GATC	6 cut(s)	458, 517, 742, 1038, 1252, 1455
BssNI	GRCGYC	1 cut(s)	1467
BssT1I	CCWWGG	3 cut(s)	592, 1031, 1459
Bst2UI	CCWGG	5 cut(s)	549, 563, 962, 1217, 1441
Bst4CI	ACNGT	5 cut(s)	307, 913, 987, 1121, 1492
BstACI	GRCGYC	1 cut(s)	1467
BstC8I	GCNNGC	3 cut(s)	14, 141, 373
BstDEI	CTNAG	7 cut(s)	210, 258, 294, 570, 1020, 1392, 1527
BstF5I	GGATG	4 cut(s)	130, 259, 693, 1191
BstKTI	GATC	6 cut(s)	461, 520, 745, 1041, 1255, 1458
BstMAI	GTCTC	2 cut(s)	36, 1458
BstMBI	GATC	6 cut(s)	458, 517, 742, 1038, 1252, 1455
BstMWI	GCNNNNNNNGC	4 cut(s)	941, 966, 1195, 1532
BstNI	CCWGG	5 cut(s)	549, 563, 962, 1217, 1441
BstSCI	CCNGG	5 cut(s)	547, 561, 960, 1215, 1439
BstSFI	CTRYAG	1 cut(s)	144
BstSLI	GKGCMC	1 cut(s)	1064
BstV1I	GCAGC	6 cut(s)	130, 336, 424, 553, 716, 1519
BstV2I	GAAGAC	1 cut(s)	1462
BstX2I	RGATCY	3 cut(s)	458, 1038, 1455
BstYI	RGATCY	3 cut(s)	458, 1038, 1455
BsuRI	GGCC	3 cut(s)	591, 1025, 1397
BtsCI	GGATG	4 cut(s)	130, 259, 693, 1191
BtsIMutI	CAGTG	7 cut(s)	303, 318, 636, 678, 918, 1406, 1488
BveI	ACCTGC	2 cut(s)	17, 137
Cac8I	GCNNGC	3 cut(s)	14, 141, 373
CaiI	CAGNNNCTG	1 cut(s)	881
CciI	TCATGA	1 cut(s)	1144
Cfr13I	GGNCC	5 cut(s)	502, 902, 1024, 1396, 1445
CseI	GACGC	1 cut(s)	215
Csp6I	GTAC	6 cut(s)	262, 391, 411, 453, 637, 1370
CspCI	CAANNNNNGTGG	2 cut(s)	894, 929
CviAII	CATG	8 cut(s)	383, 643, 811, 922, 1145, 1155, 1309, 1474
CviQI	GTAC	6 cut(s)	262, 391, 411, 453, 637, 1370
DdeI	CTNAG	7 cut(s)	210, 258, 294, 570, 1020, 1392, 1527
DpnI	GATC	6 cut(s)	460, 519, 744, 1040, 1254, 1457
DpnII	GATC	6 cut(s)	458, 517, 742, 1038, 1252, 1455
DrdI	GACNNNNNNGTC	1 cut(s)	67
DseDI	GACNNNNNNGTC	1 cut(s)	67
Eco130I	CCWWGG	3 cut(s)	592, 1031, 1459
Eco147I	AGGCCT	1 cut(s)	591
Eco47I	GGWCC	3 cut(s)	502, 902, 1445
Eco57I	CTGAAG	2 cut(s)	128, 1184
Eco88I	CYCGRG	1 cut(s)	43
EcoO109I	RGGNCCY	2 cut(s)	502, 1445
EcoRII	CCWGG	5 cut(s)	547, 561, 960, 1215, 1439
EcoT14I	CCWWGG	3 cut(s)	592, 1031, 1459
ErhI	CCWWGG	3 cut(s)	592, 1031, 1459
Esp3I	CGTCTC	1 cut(s)	1458
FaeI	CATG	8 cut(s)	386, 646, 814, 925, 1148, 1158, 1312, 1477
FalI	AAGNNNNNCTT	2 cut(s)	233, 265
FaqI	GGGAC	4 cut(s)	515, 611, 1087, 1458
FatI	CATG	8 cut(s)	382, 642, 810, 921, 1144, 1154, 1308, 1473
FblI	GTMKAC	1 cut(s)	59
Fnu4HI	GCNGC	7 cut(s)	137, 144, 325, 438, 567, 705, 1533
FokI	GGATG	4 cut(s)	137, 266, 700, 1198
Fsp4HI	GCNGC	7 cut(s)	137, 144, 325, 438, 567, 705, 1533
FspBI	CTAG	2 cut(s)	540, 1460
GluI	GCNGC	7 cut(s)	137, 144, 325, 438, 567, 705, 1533
GsuI	CTGGAG	1 cut(s)	1155
HaeIII	GGCC	3 cut(s)	591, 1025, 1397
HapII	CCGG	1 cut(s)	122
HgaI	GACGC	1 cut(s)	215
Hin1I	GRCGYC	1 cut(s)	1467
Hin1II	CATG	8 cut(s)	386, 646, 814, 925, 1148, 1158, 1312, 1477
HincII	GTYRAC	2 cut(s)	60, 1288
HindII	GTYRAC	2 cut(s)	60, 1288
HinfI	GANTC	6 cut(s)	61, 130, 404, 878, 1075, 1207
HpaII	CCGG	1 cut(s)	122
HphI	GGTGA	6 cut(s)	127, 215, 731, 827, 842, 1318
Hpy166II	GTNNAC	5 cut(s)	60, 303, 722, 1062, 1288
Hpy188I	TCNGA	1 cut(s)	1212
Hpy8I	GTNNAC	5 cut(s)	60, 303, 722, 1062, 1288
Hpy99I	CGWCG	1 cut(s)	512
HpyAV	CCTTC	5 cut(s)	106, 354, 508, 707, 1333
HpyCH4III	ACNGT	5 cut(s)	307, 913, 987, 1121, 1492
HpyCH4IV	ACGT	3 cut(s)	1297, 1428, 1467
HpyF10VI	GCNNNNNNNGC	4 cut(s)	941, 966, 1195, 1532
HpyF3I	CTNAG	7 cut(s)	210, 258, 294, 570, 1020, 1392, 1527
HpySE526I	ACGT	3 cut(s)	1297, 1428, 1467
Hsp92I	GRCGYC	1 cut(s)	1467
Hsp92II	CATG	8 cut(s)	386, 646, 814, 925, 1148, 1158, 1312, 1477
Kzo9I	GATC	6 cut(s)	458, 517, 742, 1038, 1252, 1455
LmnI	GCTCC	3 cut(s)	941, 1049, 1356
Lsp1109I	GCAGC	6 cut(s)	130, 336, 424, 553, 716, 1519
LweI	GCATC	4 cut(s)	115, 244, 1138, 1176
MaeI	CTAG	2 cut(s)	540, 1460
MaeII	ACGT	3 cut(s)	1297, 1428, 1467
MaeIII	GTNAC	3 cut(s)	149, 203, 781
MalI	GATC	6 cut(s)	460, 519, 744, 1040, 1254, 1457
MbiI	CCGCTC	1 cut(s)	1351
MboI	GATC	6 cut(s)	458, 517, 742, 1038, 1252, 1455
MboII	GAAGA	7 cut(s)	121, 253, 433, 1048, 1122, 1133, 1462
MflI	RGATCY	3 cut(s)	458, 1038, 1455
MhlI	GDGCHC	2 cut(s)	1064, 1393
MluCI	AATT	5 cut(s)	236, 273, 524, 1050, 1220
MlyI	GAGTC	2 cut(s)	55, 1069
MmeI	TCCRAC	4 cut(s)	372, 595, 929, 1498
MseI	TTAA	3 cut(s)	492, 554, 648
MslI	CAYNNNNRTG	2 cut(s)	771, 1421
MspA1I	CMGCKG	1 cut(s)	136
MspI	CCGG	1 cut(s)	122
MspR9I	CCNGG	5 cut(s)	549, 563, 962, 1217, 1441
Mva1269I	GAATGC	2 cut(s)	971, 1263
MvaI	CCWGG	5 cut(s)	549, 563, 962, 1217, 1441
MwoI	GCNNNNNNNGC	4 cut(s)	941, 966, 1195, 1532
NdeII	GATC	6 cut(s)	458, 517, 742, 1038, 1252, 1455
NlaIII	CATG	8 cut(s)	386, 646, 814, 925, 1148, 1158, 1312, 1477
NlaIV	GGNNCC	7 cut(s)	7, 342, 503, 669, 943, 1446, 1457
NmuCI	GTSAC	1 cut(s)	203
PaeR7I	CTCGAG	1 cut(s)	43
PagI	TCATGA	1 cut(s)	1144
PasI	CCCWGGG	1 cut(s)	1440
PceI	AGGCCT	1 cut(s)	591
PcsI	WCGNNNNNNNCGW	2 cut(s)	77, 404
PctI	GAATGC	2 cut(s)	971, 1263
PfeI	GAWTC	4 cut(s)	130, 404, 878, 1207
PflFI	GACNNNGTC	1 cut(s)	506
PkrI	GCNGC	7 cut(s)	138, 145, 326, 439, 568, 706, 1534
PleI	GAGTC	2 cut(s)	55, 1069
PpsI	GAGTC	2 cut(s)	55, 1069
PpuMI	RGGWCCY	2 cut(s)	502, 1445
Psp5II	RGGWCCY	2 cut(s)	502, 1445
Psp6I	CCWGG	5 cut(s)	547, 561, 960, 1215, 1439
PspGI	CCWGG	5 cut(s)	547, 561, 960, 1215, 1439
PspN4I	GGNNCC	7 cut(s)	7, 342, 503, 669, 943, 1446, 1457
PspPI	GGNCC	5 cut(s)	502, 902, 1024, 1396, 1445
PspPPI	RGGWCCY	2 cut(s)	502, 1445
PstI	CTGCAG	1 cut(s)	148
PstNI	CAGNNNCTG	1 cut(s)	881
PsuI	RGATCY	3 cut(s)	458, 1038, 1455
PsyI	GACNNNGTC	1 cut(s)	506
RsaI	GTAC	6 cut(s)	263, 392, 412, 454, 638, 1371
RsaNI	GTAC	6 cut(s)	262, 391, 411, 453, 637, 1370
RseI	CAYNNNNRTG	2 cut(s)	771, 1421
SalI	GTCGAC	1 cut(s)	58
SaqAI	TTAA	3 cut(s)	492, 554, 648
SatI	GCNGC	7 cut(s)	137, 144, 325, 438, 567, 705, 1533
Sau3AI	GATC	6 cut(s)	458, 517, 742, 1038, 1252, 1455
Sau96I	GGNCC	5 cut(s)	502, 902, 1024, 1396, 1445
ScaI	AGTACT	1 cut(s)	263
SchI	GAGTC	2 cut(s)	55, 1069
ScrFI	CCNGG	5 cut(s)	549, 563, 962, 1217, 1441
SduI	GDGCHC	2 cut(s)	1064, 1393
SfaNI	GCATC	4 cut(s)	115, 244, 1138, 1176
SfcI	CTRYAG	1 cut(s)	144
Sfr274I	CTCGAG	1 cut(s)	43
SinI	GGWCC	3 cut(s)	502, 902, 1445
SlaI	CTCGAG	1 cut(s)	43
SmiMI	CAYNNNNRTG	2 cut(s)	771, 1421
SmlI	CTYRAG	1 cut(s)	43
SmoI	CTYRAG	1 cut(s)	43
SpeI	ACTAGT	1 cut(s)	539
Sse9I	AATT	5 cut(s)	236, 273, 524, 1050, 1220
SseBI	AGGCCT	1 cut(s)	591
SsiI	CCGC	3 cut(s)	136, 694, 1349
SspMI	CTAG	2 cut(s)	540, 1460
StuI	AGGCCT	1 cut(s)	591
StyD4I	CCNGG	5 cut(s)	547, 561, 960, 1215, 1439
StyI	CCWWGG	3 cut(s)	592, 1031, 1459
TaaI	ACNGT	5 cut(s)	307, 913, 987, 1121, 1492
TaiI	ACGT	3 cut(s)	1300, 1431, 1470
TaqI	TCGA	7 cut(s)	44, 59, 170, 398, 407, 807, 1255
TasI	AATT	5 cut(s)	236, 273, 524, 1050, 1220
TatI	WGTACW	4 cut(s)	261, 390, 410, 1369
TauI	GCSGC	1 cut(s)	139
TfiI	GAWTC	4 cut(s)	130, 404, 878, 1207
Tru1I	TTAA	3 cut(s)	492, 554, 648
Tru9I	TTAA	3 cut(s)	492, 554, 648
TscAI	CASTG	7 cut(s)	310, 325, 636, 678, 918, 1406, 1495
TseFI	GTSAC	1 cut(s)	203
TseI	GCWGC	6 cut(s)	143, 324, 437, 566, 704, 1532
Tsp45I	GTSAC	1 cut(s)	203
TspDTI	ATGAA	5 cut(s)	704, 1133, 1143, 1405, 1462
TspGWI	ACGGA	1 cut(s)	1343
TspRI	CASTG	7 cut(s)	310, 325, 636, 678, 918, 1406, 1495
Tth111I	GACNNNGTC	1 cut(s)	506
VneI	GTGCAC	1 cut(s)	1060
VpaK11BI	GGWCC	3 cut(s)	502, 902, 1445
XapI	RAATTY	2 cut(s)	524, 1220
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1406
XhoI	CTCGAG	1 cut(s)	43
XmaJI	CCTAGG	1 cut(s)	1459
XmiI	GTMKAC	1 cut(s)	59
XspI	CTAG	2 cut(s)	540, 1460
ZraI	GACGTC	1 cut(s)	1468
ZrmI	AGTACT	1 cut(s)	263

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.