Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012724

Physical Location & Seq

Genomic Coordinates Reverse (-)

40499193 .. 40503625

Gene Structure 4433 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1324400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGGACGGCTTAGCTTAAAAGTCTTGTTGAGGCCAACTCAGCTGAGCTGGGGCCCACTGGTAAACGCAAAGAGTGACGGGCAGAAGAAGGTCCTCTGTTTTTCTATATTTTTAAACACTTTCTTCCTTCGAGTTTCTTCACACCAACTTTTCTCTGGCTCTCTTTCTGCTGCAAACAATTCAATCGACAATATGGGAACCAGCTTCAGGGCAATTCTGTTCCTTTCAAGCTTGTTGTTTTCTCTTGTATCCTCTGCACCCAATGATGGGTTGTCTAGGATTGGACTCAAAAGGGTGAAATTGGATTCAAATGACCGGGTTGCTGCACAGCTCGAATCCAAGAATAGATTGAGAAAGTACCGTCTCCCTTCGAATCTCGGCGAATCTGAGTCAGGGGATGTCGATATTGTCTCATTAAAGAACTACTTGGATGCTCAGTACTATGGTGAGATTGCTATTGGCACTCCCCCTCAGCCCTTCACTGTGATTTTTGACACTGGTAGCACGAACTTGTGGGTTCCATCATCCAAGTGCTATTTCTCGTTTGCATGCTACTTCCATGCCAGGTACAAGTCGGGCCAATCAAAAACATACAAGAAGAATGGGACTTCTGCTTCTATCCAGTATGGTACCGGAGCTATTTCAGGTTTCTTTAGCGAAGACAATGTTAAAGTTGGGGACATCGTTGTTAAAAACCAGGATTTTATCGAAGCAACTAGTGAGCCTGGTCTCACATTCTTGGCTGCCAAGTTTGATGGCTTATTGGGACTTGGATTTCAAGAGATTTCGGTTGGAGGTGCTGTCCCAGTGTGGTACAACATGATTAAGCAAGGTCTTATCAAGGAACCAGTGTTTTCCTTTTGGCTCAATCGTAATGCACAAGAAGAAGCAGGTGGTGAAATTGTATTTGGCGGAGTTGATCCAAATCACTATAAGGGCAAACACACGTATGTTCCAGTAACGAAGAAAGGCTATTGGCAGTTTGAGATGGGCGATGTTCTTATTGGTGGTAAACCAAGTGGATATTGTGCTGGTGGCTGTTACGCAATTGCAGACTCTGGAACTTCTCTTGTGGCAGGTCCAACAACTGTGGTTGCCCTGATAAATCAAGCCATTGGAGCCAGTGGTGTTGTTAGCCAGGAATGCAAGGCAGTTGTTAACCAGTATGGACAAACCATTCTTGATTTGCTCTTAGCTGAGGCCCAACCAAAGCAGGTTTGTTCGCAAATTGGATTGTGCACCTTTGACGGGACTCGTAGTGTAAGTATGGGCATTGAAAGCGTGGTGGATGAGAACGGCGGCAAGTCTTCTGGCATTACTCATGATGCTTCATGCTCTGCTTGTGAGATGGCAGTCGTCTGGATGCAAACCCAGCTTAAGCAGAATTTGACTCAAGATCGTATTTTGAACTACGTCAATGAGCTTTGTGAAAAAATGCCTAACCCATTGGGACAATCAGCTGTGGACTGTGGAAAGCTTTCTTCTATGCCTTCCGTTTCCTTCACCATTGGCGGTAAAGTTTTTGAGCTCACTCCAGAGGAGTACATCCTTAAGGTCGGCGAGGGCTCTCAAGCTCAGTGCATAAGCGGGTTCACTAACATGGATATCCCTCCTCCCCGTGGACCTCTCTGGATCTTGGGAGATATCTTCATGGGTCGTTACCACACCGTGTTCGATGCTGGCAAAATGAGAGTCGGATTTGCAGAGGCAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0010035 GO:0010038 GO:0016787 GO:0019538 GO:0030163 GO:0042221 GO:0043170 GO:0044238 GO:0046686 GO:0050896 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

572

Amino Acids

61.69

Weight (kDa)

6.97

Isoelectric Point (pI)

34.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	146 - 570	2.5e-114	Eukaryotic aspartyl protease
TAXi_N	PF14543	147 - 305	7.1e-12	Xylanase inhibitor N-terminal
SapB_2	PF03489	380 - 413	2.7e-13	Saposin-like type B, region 2
SapB_1	PF05184	444 - 480	3e-12	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04460 AT4G04460
fragaria_vesca	FvH4_3g01710
malus_domestica	MD05G1349700.v1.1 MD10G1324400.v1.1
prunus_persica	Prupe.4G017700_v2.0.a1 Prupe.4G017700_v2.0.a1
pyrus_communis	pycom05g31730 pycom10g27480
rosa_chinensis	RchiOBHm_Chr5g0002571
rosa_laevigata	RLG00000031040
rosa_roxburghii	Rroxscaffold_1G00073340
rosa_rugosa	Rorug04G0397000
rosa_samantha	Rh5AG020400 Rh5BG023100 Rh5CG022400 Rh5DG021500
rosa_wichuraiana	Rw5G001880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	881
Acc36I	ACCTGC	3 cut(s)	881, 1067, 1204
Acc65I	GGTACC	1 cut(s)	629
AccB1I	GGYRCC	1 cut(s)	629
AciI	CCGC	4 cut(s)	912, 1299, 1512, 1587
AclWI	GGATC	2 cut(s)	914, 1640
AcsI	RAATTY	1 cut(s)	1384
AcuI	CTGAAG	1 cut(s)	190
AdeI	CACNNNGTG	1 cut(s)	1669
AfaI	GTAC	6 cut(s)	359, 440, 569, 631, 815, 1544
AfiI	CCNNNNNNNGG	5 cut(s)	207, 266, 267, 1248, 1619
AflII	CTTAAG	2 cut(s)	1376, 1550
AflIII	ACRYGT	1 cut(s)	945
AgsI	TTSAA	6 cut(s)	183, 228, 309, 779, 1277, 1408
AhlI	ACTAGT	1 cut(s)	716
AjnI	CCWGG	4 cut(s)	563, 696, 724, 1137
AjuI	GAANNNNNNNTTGG	4 cut(s)	521, 553, 891, 923
Alw21I	GWGCWC	2 cut(s)	1241, 1530
Alw26I	GTCTC	3 cut(s)	368, 415, 734
Alw44I	GTGCAC	1 cut(s)	1237
AlwI	GGATC	2 cut(s)	914, 1640
AlwNI	CAGNNNCTG	1 cut(s)	1058
AoxI	GGCC	4 cut(s)	32, 52, 577, 1200
ApaI	GGGCCC	1 cut(s)	56
ApaLI	GTGCAC	1 cut(s)	1237
ApeKI	GCWGC	4 cut(s)	170, 323, 743, 1709
ApoI	RAATTY	1 cut(s)	1384
ArsI	GACNNNNNNTTYG	2 cut(s)	1677, 1709
Asp700I	GAANNNNTTC	1 cut(s)	1477
Asp718I	GGTACC	1 cut(s)	629
AspS9I	GGNCC	7 cut(s)	52, 53, 91, 577, 1079, 1201, 1622
AsuC2I	CCSGG	1 cut(s)	317
AsuHPI	GGTGA	4 cut(s)	307, 458, 908, 1495
AsuII	TTCGAA	1 cut(s)	371
AvaII	GGWCC	3 cut(s)	91, 1079, 1622
BaeGI	GKGCMC	2 cut(s)	56, 1241
BanI	GGYRCC	1 cut(s)	629
BanII	GRGCYC	3 cut(s)	56, 1530, 1568
BbsI	GAAGAC	2 cut(s)	666, 1299
Bbv12I	GWGCWC	2 cut(s)	1241, 1530
BbvCI	CCTCAGC	2 cut(s)	471, 1197
BbvI	GCAGC	3 cut(s)	157, 310, 730
BccI	CCATC	5 cut(s)	260, 529, 749, 982, 1342
BceAI	ACGGC	2 cut(s)	23, 1312
BciT130I	CCWGG	4 cut(s)	565, 698, 726, 1139
BciVI	GTATCC	1 cut(s)	259
BcnI	CCSGG	1 cut(s)	317
BcoDI	GTCTC	3 cut(s)	368, 415, 734
BcuI	ACTAGT	1 cut(s)	716
BfaI	CTAG	2 cut(s)	276, 717
BfrI	CTTAAG	2 cut(s)	1376, 1550
BfuAI	ACCTGC	3 cut(s)	881, 1067, 1204
BfuI	GTATCC	1 cut(s)	259
BisI	GCNGC	5 cut(s)	171, 324, 744, 1300, 1710
BlpI	GCTNAGC	2 cut(s)	11, 44
BlsI	GCNGC	5 cut(s)	172, 325, 745, 1301, 1711
BmcAI	AGTACT	1 cut(s)	440
Bme1390I	CCNGG	5 cut(s)	317, 565, 698, 726, 1139
Bme18I	GGWCC	3 cut(s)	91, 1079, 1622
BmgT120I	GGNCC	7 cut(s)	52, 53, 91, 577, 1079, 1201, 1622
BmiI	GGNNCC	7 cut(s)	53, 54, 199, 519, 631, 846, 1120
BmrFI	CCNGG	5 cut(s)	317, 565, 698, 726, 1139
BmrI	ACTGGG	1 cut(s)	800
BmsI	GCATC	4 cut(s)	421, 1315, 1353, 1666
BmuI	ACTGGG	1 cut(s)	800
BpiI	GAAGAC	2 cut(s)	666, 1299
BplI	GAGNNNNNCTC	2 cut(s)	22, 54
BpmI	CTGGAG	1 cut(s)	1518
Bpu10I	CCTNAGC	2 cut(s)	471, 1197
Bpu1102I	GCTNAGC	2 cut(s)	11, 44
Bpu14I	TTCGAA	1 cut(s)	371
BpuEI	CTTGAG	2 cut(s)	1377, 1554
BpuMI	CCSGG	1 cut(s)	317
BsaAI	YACGTR	1 cut(s)	948
BsaBI	GATNNNNATC	1 cut(s)	924
BsaI	GGTCTC	1 cut(s)	734
BsaJI	CCNNGG	1 cut(s)	1618
BsaWI	WCCGGW	1 cut(s)	632
BsaXI	ACNNNNNCTCC	2 cut(s)	786, 816
Bsc4I	CCNNNNNNNGG	5 cut(s)	207, 266, 267, 1248, 1619
Bse1I	ACTGG	8 cut(s)	63, 502, 622, 806, 848, 956, 1122, 1162
Bse8I	GATNNNNATC	1 cut(s)	924
BseBI	CCWGG	4 cut(s)	565, 698, 726, 1139
BseDI	CCNNGG	1 cut(s)	1618
BseGI	GGATG	6 cut(s)	403, 436, 524, 1294, 1368, 1545
BseJI	GATNNNNATC	1 cut(s)	924
BseLI	CCNNNNNNNGG	5 cut(s)	207, 266, 267, 1248, 1619
BseMII	CTCAG	7 cut(s)	35, 53, 378, 449, 485, 1188, 1589
BseNI	ACTGG	8 cut(s)	63, 502, 622, 806, 848, 956, 1122, 1162
BseRI	GAGGAG	2 cut(s)	1553, 1602
BseSI	GKGCMC	2 cut(s)	56, 1241
BseXI	GCAGC	3 cut(s)	157, 310, 730
BseYI	CCCAGC	2 cut(s)	48, 1371
BsgI	GTGCAG	2 cut(s)	240, 309
BshFI	GGCC	4 cut(s)	34, 54, 579, 1202
BshNI	GGYRCC	1 cut(s)	629
BsiHKAI	GWGCWC	2 cut(s)	1241, 1530
BsiSI	CCGG	2 cut(s)	316, 633
BslFI	GGGAC	7 cut(s)	18, 619, 692, 780, 788, 1264, 1464
BslI	CCNNNNNNNGG	5 cut(s)	207, 266, 267, 1248, 1619
BsmAI	GTCTC	3 cut(s)	368, 415, 734
BsmBI	CGTCTC	1 cut(s)	368
BsmFI	GGGAC	7 cut(s)	18, 619, 692, 780, 788, 1264, 1464
BsmI	GAATGC	1 cut(s)	1148
BsnI	GGCC	4 cut(s)	34, 54, 579, 1202
Bso31I	GGTCTC	1 cut(s)	734
Bsp119I	TTCGAA	1 cut(s)	371
Bsp120I	GGGCCC	1 cut(s)	52
Bsp1286I	GDGCHC	4 cut(s)	56, 1241, 1530, 1568
Bsp143I	GATC	3 cut(s)	919, 1396, 1632
Bsp1720I	GCTNAGC	2 cut(s)	11, 44
BspACI	CCGC	4 cut(s)	912, 1299, 1512, 1587
BspANI	GGCC	4 cut(s)	34, 54, 579, 1202
BspCNI	CTCAG	7 cut(s)	36, 52, 379, 448, 484, 1189, 1588
BspHI	TCATGA	1 cut(s)	1321
BspLI	GGNNCC	7 cut(s)	53, 54, 199, 519, 631, 846, 1120
BspMI	ACCTGC	3 cut(s)	881, 1067, 1204
BspPI	GGATC	2 cut(s)	914, 1640
BspT104I	TTCGAA	1 cut(s)	371
BspT107I	GGYRCC	1 cut(s)	629
BspTI	CTTAAG	2 cut(s)	1376, 1550
BspTNI	GGTCTC	1 cut(s)	734
BsrI	ACTGG	8 cut(s)	63, 502, 622, 806, 848, 956, 1122, 1162
BssECI	CCNNGG	1 cut(s)	1618
BssMI	GATC	3 cut(s)	919, 1396, 1632
Bst2UI	CCWGG	4 cut(s)	565, 698, 726, 1139
Bst4CI	ACNGT	5 cut(s)	362, 484, 1090, 1469, 1669
BstAFI	CTTAAG	2 cut(s)	1376, 1550
BstBAI	YACGTR	1 cut(s)	948
BstBI	TTCGAA	1 cut(s)	371
BstC8I	GCNNGC	2 cut(s)	550, 1681
BstDEI	CTNAG	9 cut(s)	11, 39, 44, 387, 435, 471, 1192, 1197, 1575
BstDSI	CCRYGG	1 cut(s)	1618
BstF5I	GGATG	6 cut(s)	403, 436, 524, 1294, 1368, 1545
BstKTI	GATC	3 cut(s)	922, 1399, 1635
BstMAI	GTCTC	3 cut(s)	368, 415, 734
BstMBI	GATC	3 cut(s)	919, 1396, 1632
BstMWI	GCNNNNNNNGC	6 cut(s)	40, 1118, 1143, 1278, 1372, 1709
BstNI	CCWGG	4 cut(s)	565, 698, 726, 1139
BstNSI	RCATGY	1 cut(s)	552
BstSCI	CCNGG	5 cut(s)	315, 563, 696, 724, 1137
BstSLI	GKGCMC	2 cut(s)	56, 1241
BstV1I	GCAGC	3 cut(s)	157, 310, 730
BstV2I	GAAGAC	2 cut(s)	666, 1299
BstX2I	RGATCY	1 cut(s)	1632
BstYI	RGATCY	1 cut(s)	1632
BsuI	GTATCC	1 cut(s)	259
BsuRI	GGCC	4 cut(s)	34, 54, 579, 1202
BtgI	CCRYGG	1 cut(s)	1618
BtgZI	GCGATG	1 cut(s)	1008
BtsCI	GGATG	6 cut(s)	403, 436, 524, 1294, 1368, 1545
BtsIMutI	CAGTG	7 cut(s)	56, 480, 495, 813, 855, 1129, 1583
BveI	ACCTGC	3 cut(s)	881, 1067, 1204
Cac8I	GCNNGC	2 cut(s)	550, 1681
CaiI	CAGNNNCTG	1 cut(s)	1058
CciI	TCATGA	1 cut(s)	1321
Cfr13I	GGNCC	7 cut(s)	52, 53, 91, 577, 1079, 1201, 1622
Csp6I	GTAC	6 cut(s)	358, 439, 568, 630, 814, 1543
CspCI	CAANNNNNGTGG	2 cut(s)	1071, 1106
CviAII	CATG	7 cut(s)	549, 560, 820, 1322, 1332, 1600, 1651
CviQI	GTAC	6 cut(s)	358, 439, 568, 630, 814, 1543
DdeI	CTNAG	9 cut(s)	11, 39, 44, 387, 435, 471, 1192, 1197, 1575
DpnI	GATC	3 cut(s)	921, 1398, 1634
DpnII	GATC	3 cut(s)	919, 1396, 1632
DraI	TTTAAA	1 cut(s)	114
DraIII	CACNNNGTG	1 cut(s)	1669
EciI	GGCGGA	1 cut(s)	927
Ecl136II	GAGCTC	1 cut(s)	1528
Eco24I	GRGCYC	3 cut(s)	56, 1530, 1568
Eco31I	GGTCTC	1 cut(s)	734
Eco32I	GATATC	2 cut(s)	1606, 1645
Eco47I	GGWCC	3 cut(s)	91, 1079, 1622
Eco53kI	GAGCTC	1 cut(s)	1528
Eco57I	CTGAAG	1 cut(s)	190
EcoICRI	GAGCTC	1 cut(s)	1528
EcoO109I	RGGNCCY	2 cut(s)	52, 91
EcoRII	CCWGG	4 cut(s)	563, 696, 724, 1137
EcoRV	GATATC	2 cut(s)	1606, 1645
EcoT38I	GRGCYC	3 cut(s)	56, 1530, 1568
Esp3I	CGTCTC	1 cut(s)	368
FaeI	CATG	7 cut(s)	552, 563, 823, 1325, 1335, 1603, 1654
FalI	AAGNNNNNCTT	2 cut(s)	410, 442
FaqI	GGGAC	7 cut(s)	18, 619, 692, 780, 788, 1264, 1464
FatI	CATG	7 cut(s)	548, 559, 819, 1321, 1331, 1599, 1650
FauI	CCCGC	1 cut(s)	1580
Fnu4HI	GCNGC	5 cut(s)	171, 324, 744, 1300, 1710
FokI	GGATG	6 cut(s)	410, 443, 511, 1301, 1375, 1532
FriOI	GRGCYC	3 cut(s)	56, 1530, 1568
Fsp4HI	GCNGC	5 cut(s)	171, 324, 744, 1300, 1710
FspBI	CTAG	2 cut(s)	276, 717
GluI	GCNGC	5 cut(s)	171, 324, 744, 1300, 1710
GsaI	CCCAGC	2 cut(s)	52, 1375
GsuI	CTGGAG	1 cut(s)	1518
HaeIII	GGCC	4 cut(s)	34, 54, 579, 1202
HapII	CCGG	2 cut(s)	316, 633
Hin1II	CATG	7 cut(s)	552, 563, 823, 1325, 1335, 1603, 1654
HincII	GTYRAC	1 cut(s)	1159
HindII	GTYRAC	1 cut(s)	1159
HindIII	AAGCTT	2 cut(s)	229, 1475
HpaI	GTTAAC	1 cut(s)	1159
HpaII	CCGG	2 cut(s)	316, 633
HphI	GGTGA	4 cut(s)	307, 458, 908, 1495
Hpy166II	GTNNAC	7 cut(s)	64, 1013, 1159, 1239, 1465, 1593, 1622
Hpy188I	TCNGA	2 cut(s)	388, 1697
Hpy188III	TCNNGA	8 cut(s)	779, 1059, 1181, 1322, 1360, 1394, 1535, 1630
Hpy8I	GTNNAC	7 cut(s)	64, 1013, 1159, 1239, 1465, 1593, 1622
HpyAV	CCTTC	6 cut(s)	82, 137, 378, 487, 1500, 1510
HpyCH4III	ACNGT	5 cut(s)	362, 484, 1090, 1469, 1669
HpyCH4IV	ACGT	2 cut(s)	947, 1413
HpyF10VI	GCNNNNNNNGC	6 cut(s)	40, 1118, 1143, 1278, 1372, 1709
HpyF3I	CTNAG	9 cut(s)	11, 39, 44, 387, 435, 471, 1192, 1197, 1575
HpySE526I	ACGT	2 cut(s)	947, 1413
Hsp92II	CATG	7 cut(s)	552, 563, 823, 1325, 1335, 1603, 1654
KpnI	GGTACC	1 cut(s)	633
KspAI	GTTAAC	1 cut(s)	1159
Kzo9I	GATC	3 cut(s)	919, 1396, 1632
LmnI	GCTCC	2 cut(s)	635, 1118
Lsp1109I	GCAGC	3 cut(s)	157, 310, 730
LweI	GCATC	4 cut(s)	421, 1315, 1353, 1666
MaeI	CTAG	2 cut(s)	276, 717
MaeII	ACGT	2 cut(s)	947, 1413
MaeIII	GTNAC	4 cut(s)	74, 958, 1040, 1658
MalI	GATC	3 cut(s)	921, 1398, 1634
MboI	GATC	3 cut(s)	919, 1396, 1632
MfeI	CAATTG	1 cut(s)	1047
MflI	RGATCY	1 cut(s)	1632
MhlI	GDGCHC	4 cut(s)	56, 1241, 1530, 1568
MluCI	AATT	7 cut(s)	178, 213, 299, 900, 1047, 1227, 1384
MlyI	GAGTC	6 cut(s)	279, 398, 1049, 1246, 1384, 1701
MmeI	TCCRAC	3 cut(s)	772, 1106, 1675
MroXI	GAANNNNTTC	1 cut(s)	1477
MseI	TTAA	9 cut(s)	17, 113, 416, 669, 690, 825, 1158, 1377, 1551
MslI	CAYNNNNRTG	3 cut(s)	529, 948, 1598
MspA1I	CMGCKG	2 cut(s)	43, 1460
MspCI	CTTAAG	2 cut(s)	1376, 1550
MspI	CCGG	2 cut(s)	316, 633
MspR9I	CCNGG	5 cut(s)	317, 565, 698, 726, 1139
MunI	CAATTG	1 cut(s)	1047
Mva1269I	GAATGC	1 cut(s)	1148
MvaI	CCWGG	4 cut(s)	565, 698, 726, 1139
MwoI	GCNNNNNNNGC	6 cut(s)	40, 1118, 1143, 1278, 1372, 1709
NciI	CCSGG	1 cut(s)	317
NdeII	GATC	3 cut(s)	919, 1396, 1632
NlaIII	CATG	7 cut(s)	552, 563, 823, 1325, 1335, 1603, 1654
NlaIV	GGNNCC	7 cut(s)	53, 54, 199, 519, 631, 846, 1120
NmeAIII	GCCGAG	1 cut(s)	357
NmuCI	GTSAC	1 cut(s)	74
NspI	RCATGY	1 cut(s)	552
NspV	TTCGAA	1 cut(s)	371
PaeI	GCATGC	1 cut(s)	552
PagI	TCATGA	1 cut(s)	1321
PaqCI	CACCTGC	1 cut(s)	881
PctI	GAATGC	1 cut(s)	1148
PdmI	GAANNNNTTC	1 cut(s)	1477
PfeI	GAWTC	4 cut(s)	305, 335, 373, 383
PkrI	GCNGC	5 cut(s)	172, 325, 745, 1301, 1711
PleI	GAGTC	6 cut(s)	279, 397, 1049, 1246, 1384, 1700
PpsI	GAGTC	6 cut(s)	279, 397, 1049, 1246, 1384, 1700
Ppu21I	YACGTR	1 cut(s)	948
PpuMI	RGGWCCY	1 cut(s)	91
Psp124BI	GAGCTC	1 cut(s)	1530
Psp5II	RGGWCCY	1 cut(s)	91
Psp6I	CCWGG	4 cut(s)	563, 696, 724, 1137
PspFI	CCCAGC	2 cut(s)	48, 1371
PspGI	CCWGG	4 cut(s)	563, 696, 724, 1137
PspN4I	GGNNCC	7 cut(s)	53, 54, 199, 519, 631, 846, 1120
PspOMI	GGGCCC	1 cut(s)	52
PspPI	GGNCC	7 cut(s)	52, 53, 91, 577, 1079, 1201, 1622
PspPPI	RGGWCCY	1 cut(s)	91
PstNI	CAGNNNCTG	1 cut(s)	1058
PsuI	RGATCY	1 cut(s)	1632
PvuII	CAGCTG	2 cut(s)	43, 1460
RsaI	GTAC	6 cut(s)	359, 440, 569, 631, 815, 1544
RsaNI	GTAC	6 cut(s)	358, 439, 568, 630, 814, 1543
RseI	CAYNNNNRTG	3 cut(s)	529, 948, 1598
SacI	GAGCTC	1 cut(s)	1530
SaqAI	TTAA	9 cut(s)	17, 113, 416, 669, 690, 825, 1158, 1377, 1551
SatI	GCNGC	5 cut(s)	171, 324, 744, 1300, 1710
Sau3AI	GATC	3 cut(s)	919, 1396, 1632
Sau96I	GGNCC	7 cut(s)	52, 53, 91, 577, 1079, 1201, 1622
ScaI	AGTACT	1 cut(s)	440
SchI	GAGTC	6 cut(s)	279, 398, 1049, 1246, 1384, 1701
ScrFI	CCNGG	5 cut(s)	317, 565, 698, 726, 1139
SduI	GDGCHC	4 cut(s)	56, 1241, 1530, 1568
SfaNI	GCATC	4 cut(s)	421, 1315, 1353, 1666
SfuI	TTCGAA	1 cut(s)	371
SinI	GGWCC	3 cut(s)	91, 1079, 1622
SmiMI	CAYNNNNRTG	3 cut(s)	529, 948, 1598
SmlI	CTYRAG	4 cut(s)	1376, 1392, 1550, 1569
SmoI	CTYRAG	4 cut(s)	1376, 1392, 1550, 1569
SpeI	ACTAGT	1 cut(s)	716
SphI	GCATGC	1 cut(s)	552
Sse9I	AATT	7 cut(s)	178, 213, 299, 900, 1047, 1227, 1384
SsiI	CCGC	4 cut(s)	912, 1299, 1512, 1587
SspMI	CTAG	2 cut(s)	276, 717
SstI	GAGCTC	1 cut(s)	1530
StyD4I	CCNGG	5 cut(s)	315, 563, 696, 724, 1137
TaaI	ACNGT	5 cut(s)	362, 484, 1090, 1469, 1669
TaiI	ACGT	2 cut(s)	950, 1416
TaqI	TCGA	7 cut(s)	130, 186, 333, 371, 402, 708, 1674
TasI	AATT	7 cut(s)	178, 213, 299, 900, 1047, 1227, 1384
TatI	WGTACW	2 cut(s)	438, 1542
TauI	GCSGC	1 cut(s)	1302
TfiI	GAWTC	4 cut(s)	305, 335, 373, 383
Tru1I	TTAA	9 cut(s)	17, 113, 416, 669, 690, 825, 1158, 1377, 1551
Tru9I	TTAA	9 cut(s)	17, 113, 416, 669, 690, 825, 1158, 1377, 1551
TscAI	CASTG	7 cut(s)	63, 487, 502, 813, 855, 1129, 1583
TseFI	GTSAC	1 cut(s)	74
TseI	GCWGC	4 cut(s)	170, 323, 743, 1709
Tsp45I	GTSAC	1 cut(s)	74
TspDTI	ATGAA	2 cut(s)	1320, 1639
TspGWI	ACGGA	1 cut(s)	1483
TspRI	CASTG	7 cut(s)	63, 487, 502, 813, 855, 1129, 1583
Vha464I	CTTAAG	2 cut(s)	1376, 1550
VneI	GTGCAC	1 cut(s)	1237
VpaK11BI	GGWCC	3 cut(s)	91, 1079, 1622
XapI	RAATTY	1 cut(s)	1384
XceI	RCATGY	1 cut(s)	552
XcmI	CCANNNNNNNNNTGG	1 cut(s)	152
XmnI	GAANNNNTTC	1 cut(s)	1477
XspI	CTAG	2 cut(s)	276, 717
ZrmI	AGTACT	1 cut(s)	440

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.