Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0012724

Physical Location & Seq

Genomic Coordinates Forward (+)

1628875 .. 1632750

Gene Structure 3876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G001880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGGAACCAGCTTCCAAGGGGTAGTGTTGTCTCTGTTCGTTTCGAGCTTGTTGTTGTCTCTGGTGTCCTCTGCAGCCCCACCCAGTGACGGGTTGTTTAGGATTGGACTGAAAAAGGTGAAGTTGGATGCGAATGAGCGGCTTGCTGCAGCGGTTGAATACAAGAATGGTGAGGATTTGAGGGGTTCGATTAGGAAGTATGGCTTCCCTTCTAATCTGGGAGACGCTGAGGGGGAGGATATTGATATTGTCAAATTAAAGAACTACTTGGATGCTCAGTACTATGGTGAGATAGCTATTGGCACTCCCCCTCAGCCGTTCACTGTGATTTTCGACACTGGCAGCTCTAATCTGTGGGTTCCGTCGAACAAGTGTCATTTCTCGGTTGCGTGCTACTTCCATGCCAAGTACAAGTCAAGCCAATCGAGTACATACAAGAAGAATGGGAAATCTGCTTCCATCCAGTATGGTACTGGAGCTATTTCAGGTTTCTTTAGTAATGACAATGTTAAAGTTGGGGACCTCGTTGTGAAAGACCAGGAATTTATTGAAGCAACTAGTGAGCCTGGTCTAACATTTTTGGCTGCCAAGTTTGATGGGCTATTAGGCCTTGGATTTCAAGAGATTTCAGTTGGAAATGCTGTCCCAGTGTGGTACAACATGATTAAGCAAGGGCTTATCAAGGAACCAGTGTTTTCCTTTTGGATGAACCGCAATGCAGCAGCACAAGAAGGTGGTGAACTTGTGTTTGGGGGAGTTGATCCCCATCACTTCAAGGGAAAGCACACATATGTTCCTGTAACACAGAAAGGCTATTGGCAGTTCAACATGGGTGATGTTCTTATTGGTGATAAACCAACTGGATATTGTGCTGGTGGTTGTCATGCAATTGCAGATTCTGGAACTTCTCTTTTGGCTGGTCCAACGACAGTGGTTACATTGATAAATAAAGCCATTGGAGCCAGTGGGGTTGTTAGCCAGGAATGCAAGGCTGTTGTTCAACAGTATGGTCAAACCATTATGGAGCTGCTCTTGGCTGAGGCTCAACCAAAGAAGATCTGCTCCCAAATTGGAGTGTGCACCTTTGATGGGAGTCGTAGTGTTGGTATGGGCATTGAGAGTGTGGTGGACAAGAACCGTGGAAAATCTTCTGGAGTTCTTCATGATGCTTCATGCTCTGTTTGTGAGATGGCAGTTGTCTGGATGCAAAACCAGCTTAAGCAGAATCAGACCCAGGATTATATTTTGAACTATGTTAATGAGCTTTGTGATCAAATGCCAAGCCCAAATGGACAATCGGCTGTTGACTGCCAACGTCTTTCTTCCATGCCTACCATTTCCTTCACCATTGGTGGTAAAGTTTTTCCGCTCACCCCAGAGGAGTATGTACTCAAGGTGGGCGAGGGTCCACAAGCCCAGTGCATAAGTGGCTTCACTAGTATGGACATTCCTCCTCCCCGGGGACCTCTCTGGATCCTGGGAGACGTCTTCATGGGGCGCTACCACACTGTCTTTGATTATGGTAATATGAGAGTTGGATTTGCAAAGGCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0010035 GO:0010038 GO:0016787 GO:0019538 GO:0030163 GO:0042221 GO:0043170 GO:0044238 GO:0046686 GO:0050896 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

518

Amino Acids

55.88

Weight (kDa)

6.1

Isoelectric Point (pI)

33.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	93 - 517	7.9e-118	Eukaryotic aspartyl protease
TAXi_N	PF14543	94 - 252	8.1e-12	Xylanase inhibitor N-terminal
SapB_2	PF03489	327 - 360	1.3e-11	Saposin-like type B, region 2
SapB_1	PF05184	391 - 427	8.4e-14	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04460 AT4G04460
fragaria_vesca	FvH4_3g01710
malus_domestica	MD05G1349700.v1.1 MD10G1324400.v1.1
prunus_persica	Prupe.4G017700_v2.0.a1 Prupe.4G017700_v2.0.a1
pyrus_communis	pycom05g31730 pycom10g27480
rosa_chinensis	RchiOBHm_Chr5g0002571
rosa_laevigata	RLG00000031040
rosa_roxburghii	Rroxscaffold_1G00073340
rosa_rugosa	Rorug04G0397000
rosa_samantha	Rh5AG020400 Rh5BG023100 Rh5CG022400 Rh5DG021500
rosa_wichuraiana	Rw5G001880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1488
AccBSI	CCGCTC	2 cut(s)	139, 1369
AciI	CCGC	4 cut(s)	139, 152, 712, 1367
AclWI	GGATC	3 cut(s)	755, 1468, 1481
AcsI	RAATTY	1 cut(s)	542
AcyI	GRCGYC	1 cut(s)	1485
AdeI	CACNNNGTG	1 cut(s)	86
AfaI	GTAC	6 cut(s)	281, 410, 430, 472, 656, 1389
AfiI	CCNNNNNNNGG	4 cut(s)	89, 90, 1089, 1460
AflII	CTTAAG	1 cut(s)	1217
AgsI	TTSAA	7 cut(s)	158, 551, 620, 775, 826, 1001, 1249
AhlI	ACTAGT	2 cut(s)	557, 1436
AjnI	CCWGG	5 cut(s)	537, 565, 978, 1233, 1476
AjuI	GAANNNNNNNTTGG	2 cut(s)	732, 764
AluBI	AGCT	8 cut(s)	12, 48, 296, 345, 479, 1027, 1216, 1264
AluI	AGCT	8 cut(s)	12, 48, 296, 345, 479, 1027, 1216, 1264
Alw21I	GWGCWC	1 cut(s)	1082
Alw26I	GTCTC	4 cut(s)	36, 63, 216, 1476
Alw44I	GTGCAC	1 cut(s)	1078
AlwI	GGATC	3 cut(s)	755, 1468, 1481
AlwNI	CAGNNNCTG	1 cut(s)	899
Ama87I	CYCGRG	1 cut(s)	1458
AoxI	GGCC	1 cut(s)	607
ApaLI	GTGCAC	1 cut(s)	1078
ApeKI	GCWGC	9 cut(s)	74, 146, 149, 342, 584, 719, 722, 1027, 1550
ApoI	RAATTY	1 cut(s)	542
Asp700I	GAANNNNTTC	1 cut(s)	1147
AspLEI	GCGC	1 cut(s)	1500
AspS9I	GGNCC	4 cut(s)	520, 920, 1406, 1463
AsuC2I	CCSGG	2 cut(s)	1459, 1460
AsuHPI	GGTGA	8 cut(s)	130, 182, 299, 749, 845, 860, 1336, 1363
AvaI	CYCGRG	1 cut(s)	1458
AvaII	GGWCC	4 cut(s)	520, 920, 1406, 1463
BaeGI	GKGCMC	1 cut(s)	1082
BamHI	GGATCC	1 cut(s)	1473
BbsI	GAAGAC	1 cut(s)	1480
Bbv12I	GWGCWC	1 cut(s)	1082
BbvCI	CCTCAGC	3 cut(s)	228, 312, 1038
BbvI	GCAGC	9 cut(s)	86, 133, 161, 354, 571, 731, 734, 1014, 1537
BccI	CCATC	5 cut(s)	467, 590, 774, 1082, 1183
BceAI	ACGGC	1 cut(s)	301
BciT130I	CCWGG	5 cut(s)	539, 567, 980, 1235, 1478
BclI	TGATCA	1 cut(s)	1270
BcnI	CCSGG	2 cut(s)	1459, 1460
BcoDI	GTCTC	4 cut(s)	36, 63, 216, 1476
BcuI	ACTAGT	2 cut(s)	557, 1436
BfaI	CTAG	2 cut(s)	558, 1437
BfmI	CTRYAG	2 cut(s)	72, 147
BfoI	RGCGCY	1 cut(s)	1501
BfrI	CTTAAG	1 cut(s)	1217
BglII	AGATCT	1 cut(s)	1056
BmcAI	AGTACT	1 cut(s)	281
Bme1390I	CCNGG	7 cut(s)	539, 567, 980, 1235, 1459, 1460, 1478
Bme18I	GGWCC	4 cut(s)	520, 920, 1406, 1463
BmeT110I	CYCGRG	1 cut(s)	1458
BmgT120I	GGNCC	4 cut(s)	520, 920, 1406, 1463
BmiI	GGNNCC	8 cut(s)	7, 360, 521, 687, 961, 1407, 1464, 1475
BmrFI	CCNGG	7 cut(s)	539, 567, 980, 1235, 1459, 1460, 1478
BmrI	ACTGGG	3 cut(s)	78, 641, 1411
BmsI	GCATC	4 cut(s)	118, 262, 1156, 1194
BmuI	ACTGGG	3 cut(s)	78, 641, 1411
BpiI	GAAGAC	1 cut(s)	1480
BpmI	CTGGAG	2 cut(s)	495, 1173
Bpu10I	CCTNAGC	3 cut(s)	228, 312, 1038
BpuEI	CTTGAG	1 cut(s)	1376
BpuMI	CCSGG	2 cut(s)	1459, 1460
BsaBI	GATNNNNATC	1 cut(s)	765
BsaHI	GRCGYC	1 cut(s)	1485
BsaJI	CCNNGG	8 cut(s)	16, 610, 1138, 1233, 1457, 1458, 1459, 1477
BsaXI	ACNNNNNCTCC	2 cut(s)	1084, 1114
Bsc4I	CCNNNNNNNGG	4 cut(s)	89, 90, 1089, 1460
Bse1I	ACTGG	9 cut(s)	84, 343, 463, 478, 647, 689, 865, 963, 1417
Bse3DI	GCAATG	1 cut(s)	721
Bse8I	GATNNNNATC	1 cut(s)	765
BseBI	CCWGG	5 cut(s)	539, 567, 980, 1235, 1478
BseDI	CCNNGG	8 cut(s)	16, 610, 1138, 1233, 1457, 1458, 1459, 1477
BseGI	GGATG	5 cut(s)	133, 277, 459, 711, 1209
BseJI	GATNNNNATC	1 cut(s)	765
BseLI	CCNNNNNNNGG	4 cut(s)	89, 90, 1089, 1460
BseMI	GCAATG	1 cut(s)	721
BseMII	CTCAG	4 cut(s)	219, 290, 326, 1029
BseNI	ACTGG	9 cut(s)	84, 343, 463, 478, 647, 689, 865, 963, 1417
BseRI	GAGGAG	2 cut(s)	1394, 1443
BseSI	GKGCMC	1 cut(s)	1082
BseXI	GCAGC	9 cut(s)	86, 133, 161, 354, 571, 731, 734, 1014, 1537
BshFI	GGCC	1 cut(s)	609
BsiHKAI	GWGCWC	1 cut(s)	1082
BsiHKCI	CYCGRG	1 cut(s)	1458
BsiSI	CCGG	1 cut(s)	1459
BslFI	GGGAC	3 cut(s)	533, 629, 1476
BslI	CCNNNNNNNGG	4 cut(s)	89, 90, 1089, 1460
BsmAI	GTCTC	4 cut(s)	36, 63, 216, 1476
BsmBI	CGTCTC	2 cut(s)	216, 1476
BsmFI	GGGAC	3 cut(s)	533, 629, 1476
BsmI	GAATGC	1 cut(s)	989
BsnI	GGCC	1 cut(s)	609
BsoBI	CYCGRG	1 cut(s)	1458
Bsp1286I	GDGCHC	1 cut(s)	1082
Bsp143I	GATC	4 cut(s)	760, 1056, 1270, 1473
BspACI	CCGC	4 cut(s)	139, 152, 712, 1367
BspANI	GGCC	1 cut(s)	609
BspCNI	CTCAG	4 cut(s)	220, 289, 325, 1030
BspHI	TCATGA	1 cut(s)	1162
BspLI	GGNNCC	8 cut(s)	7, 360, 521, 687, 961, 1407, 1464, 1475
BspMAI	CTGCAG	2 cut(s)	76, 151
BspPI	GGATC	3 cut(s)	755, 1468, 1481
BspTI	CTTAAG	1 cut(s)	1217
BsrBI	CCGCTC	2 cut(s)	139, 1369
BsrDI	GCAATG	1 cut(s)	721
BsrI	ACTGG	9 cut(s)	84, 343, 463, 478, 647, 689, 865, 963, 1417
BssECI	CCNNGG	8 cut(s)	16, 610, 1138, 1233, 1457, 1458, 1459, 1477
BssMI	GATC	4 cut(s)	760, 1056, 1270, 1473
BssNI	GRCGYC	1 cut(s)	1485
BssT1I	CCWWGG	2 cut(s)	16, 610
Bst2UI	CCWGG	5 cut(s)	539, 567, 980, 1235, 1478
Bst4CI	ACNGT	5 cut(s)	325, 931, 1005, 1139, 1510
BstACI	GRCGYC	1 cut(s)	1485
BstAFI	CTTAAG	1 cut(s)	1217
BstAPI	GCANNNNNTGC	1 cut(s)	1550
BstC8I	GCNNGC	2 cut(s)	144, 391
BstDEI	CTNAG	4 cut(s)	228, 276, 312, 1038
BstDSI	CCRYGG	1 cut(s)	1138
BstF5I	GGATG	5 cut(s)	133, 277, 459, 711, 1209
BstH2I	RGCGCY	1 cut(s)	1501
BstHHI	GCGC	1 cut(s)	1500
BstKTI	GATC	4 cut(s)	763, 1059, 1273, 1476
BstMAI	GTCTC	4 cut(s)	36, 63, 216, 1476
BstMBI	GATC	4 cut(s)	760, 1056, 1270, 1473
BstMWI	GCNNNNNNNGC	5 cut(s)	959, 984, 1213, 1428, 1550
BstNI	CCWGG	5 cut(s)	539, 567, 980, 1235, 1478
BstSCI	CCNGG	7 cut(s)	537, 565, 978, 1233, 1457, 1458, 1476
BstSFI	CTRYAG	2 cut(s)	72, 147
BstSLI	GKGCMC	1 cut(s)	1082
BstV1I	GCAGC	9 cut(s)	86, 133, 161, 354, 571, 731, 734, 1014, 1537
BstV2I	GAAGAC	1 cut(s)	1480
BstX2I	RGATCY	2 cut(s)	1056, 1473
BstYI	RGATCY	2 cut(s)	1056, 1473
BsuRI	GGCC	1 cut(s)	609
BtgI	CCRYGG	1 cut(s)	1138
BtsCI	GGATG	5 cut(s)	133, 277, 459, 711, 1209
BtsIMutI	CAGTG	9 cut(s)	91, 321, 336, 654, 696, 936, 970, 1424, 1506
Cac8I	GCNNGC	2 cut(s)	144, 391
CaiI	CAGNNNCTG	1 cut(s)	899
CciI	TCATGA	1 cut(s)	1162
CfoI	GCGC	1 cut(s)	1500
Cfr13I	GGNCC	4 cut(s)	520, 920, 1406, 1463
Cfr9I	CCCGGG	1 cut(s)	1458
CseI	GACGC	1 cut(s)	233
Csp6I	GTAC	6 cut(s)	280, 409, 429, 471, 655, 1388
CspCI	CAANNNNNGTGG	4 cut(s)	912, 947, 1120, 1155
CviAII	CATG	8 cut(s)	401, 661, 829, 884, 1163, 1173, 1327, 1492
CviQI	GTAC	6 cut(s)	280, 409, 429, 471, 655, 1388
DdeI	CTNAG	4 cut(s)	228, 276, 312, 1038
DpnI	GATC	4 cut(s)	762, 1058, 1272, 1475
DpnII	GATC	4 cut(s)	760, 1056, 1270, 1473
DraIII	CACNNNGTG	1 cut(s)	86
Eco130I	CCWWGG	2 cut(s)	16, 610
Eco147I	AGGCCT	1 cut(s)	609
Eco47I	GGWCC	4 cut(s)	520, 920, 1406, 1463
Eco88I	CYCGRG	1 cut(s)	1458
EcoO109I	RGGNCCY	2 cut(s)	520, 1463
EcoRII	CCWGG	5 cut(s)	537, 565, 978, 1233, 1476
EcoT14I	CCWWGG	2 cut(s)	16, 610
ErhI	CCWWGG	2 cut(s)	16, 610
Esp3I	CGTCTC	2 cut(s)	216, 1476
FaeI	CATG	8 cut(s)	404, 664, 832, 887, 1166, 1176, 1330, 1495
FalI	AAGNNNNNCTT	4 cut(s)	188, 220, 251, 283
FaqI	GGGAC	3 cut(s)	533, 629, 1476
FatI	CATG	8 cut(s)	400, 660, 828, 883, 1162, 1172, 1326, 1491
FauNDI	CATATG	1 cut(s)	790
FbaI	TGATCA	1 cut(s)	1270
FokI	GGATG	5 cut(s)	140, 284, 446, 718, 1216
FspBI	CTAG	2 cut(s)	558, 1437
GlaI	GCGC	1 cut(s)	1499
GsuI	CTGGAG	2 cut(s)	495, 1173
HaeII	RGCGCY	1 cut(s)	1501
HaeIII	GGCC	1 cut(s)	609
HapII	CCGG	1 cut(s)	1459
HgaI	GACGC	1 cut(s)	233
HhaI	GCGC	1 cut(s)	1500
Hin1I	GRCGYC	1 cut(s)	1485
Hin1II	CATG	8 cut(s)	404, 664, 832, 887, 1166, 1176, 1330, 1495
Hin6I	GCGC	1 cut(s)	1498
HinP1I	GCGC	1 cut(s)	1498
HincII	GTYRAC	1 cut(s)	1306
HindII	GTYRAC	1 cut(s)	1306
HinfI	GANTC	3 cut(s)	896, 1093, 1225
HpaII	CCGG	1 cut(s)	1459
HphI	GGTGA	8 cut(s)	130, 182, 299, 749, 845, 860, 1336, 1363
Hpy166II	GTNNAC	6 cut(s)	321, 740, 1080, 1129, 1306, 1409
Hpy188I	TCNGA	1 cut(s)	1230
Hpy188III	TCNNGA	6 cut(s)	620, 900, 1152, 1163, 1201, 1471
Hpy8I	GTNNAC	6 cut(s)	321, 740, 1080, 1129, 1306, 1409
Hpy99I	CGWCG	1 cut(s)	367
HpyAV	CCTTC	3 cut(s)	219, 725, 1351
HpyCH4III	ACNGT	5 cut(s)	325, 931, 1005, 1139, 1510
HpyCH4IV	ACGT	2 cut(s)	1315, 1485
HpyF10VI	GCNNNNNNNGC	5 cut(s)	959, 984, 1213, 1428, 1550
HpyF3I	CTNAG	4 cut(s)	228, 276, 312, 1038
HpySE526I	ACGT	2 cut(s)	1315, 1485
Hsp92I	GRCGYC	1 cut(s)	1485
Hsp92II	CATG	8 cut(s)	404, 664, 832, 887, 1166, 1176, 1330, 1495
HspAI	GCGC	1 cut(s)	1498
Ksp22I	TGATCA	1 cut(s)	1270
Kzo9I	GATC	4 cut(s)	760, 1056, 1270, 1473
LmnI	GCTCC	4 cut(s)	476, 959, 1024, 1067
Lsp1109I	GCAGC	9 cut(s)	86, 133, 161, 354, 571, 731, 734, 1014, 1537
LweI	GCATC	4 cut(s)	118, 262, 1156, 1194
MaeI	CTAG	2 cut(s)	558, 1437
MaeII	ACGT	2 cut(s)	1315, 1485
MaeIII	GTNAC	3 cut(s)	86, 799, 934
MalI	GATC	4 cut(s)	762, 1058, 1272, 1475
MbiI	CCGCTC	2 cut(s)	139, 1369
MboI	GATC	4 cut(s)	760, 1056, 1270, 1473
MboII	GAAGA	6 cut(s)	451, 1066, 1140, 1151, 1314, 1480
MfeI	CAATTG	1 cut(s)	888
MflI	RGATCY	2 cut(s)	1056, 1473
MhlI	GDGCHC	1 cut(s)	1082
MluCI	AATT	4 cut(s)	254, 542, 888, 1068
MlyI	GAGTC	1 cut(s)	1102
MmeI	TCCRAC	4 cut(s)	105, 613, 947, 1516
MroXI	GAANNNNTTC	1 cut(s)	1147
MseI	TTAA	5 cut(s)	257, 510, 666, 1218, 1257
MslI	CAYNNNNRTG	2 cut(s)	789, 1439
MspA1I	CMGCKG	1 cut(s)	152
MspCI	CTTAAG	1 cut(s)	1217
MspI	CCGG	1 cut(s)	1459
MspR9I	CCNGG	7 cut(s)	539, 567, 980, 1235, 1459, 1460, 1478
MunI	CAATTG	1 cut(s)	888
Mva1269I	GAATGC	1 cut(s)	989
MvaI	CCWGG	5 cut(s)	539, 567, 980, 1235, 1478
MwoI	GCNNNNNNNGC	5 cut(s)	959, 984, 1213, 1428, 1550
NciI	CCSGG	2 cut(s)	1459, 1460
NdeI	CATATG	1 cut(s)	790
NdeII	GATC	4 cut(s)	760, 1056, 1270, 1473
NlaIII	CATG	8 cut(s)	404, 664, 832, 887, 1166, 1176, 1330, 1495
NlaIV	GGNNCC	8 cut(s)	7, 360, 521, 687, 961, 1407, 1464, 1475
NmuCI	GTSAC	1 cut(s)	86
PagI	TCATGA	1 cut(s)	1162
PceI	AGGCCT	1 cut(s)	609
PctI	GAATGC	1 cut(s)	989
PdmI	GAANNNNTTC	1 cut(s)	1147
PfeI	GAWTC	2 cut(s)	896, 1225
PleI	GAGTC	1 cut(s)	1101
PpsI	GAGTC	1 cut(s)	1101
PpuMI	RGGWCCY	2 cut(s)	520, 1463
Psp5II	RGGWCCY	2 cut(s)	520, 1463
Psp6I	CCWGG	5 cut(s)	537, 565, 978, 1233, 1476
PspGI	CCWGG	5 cut(s)	537, 565, 978, 1233, 1476
PspN4I	GGNNCC	8 cut(s)	7, 360, 521, 687, 961, 1407, 1464, 1475
PspPI	GGNCC	4 cut(s)	520, 920, 1406, 1463
PspPPI	RGGWCCY	2 cut(s)	520, 1463
PstI	CTGCAG	2 cut(s)	76, 151
PstNI	CAGNNNCTG	1 cut(s)	899
PsuI	RGATCY	2 cut(s)	1056, 1473
RsaI	GTAC	6 cut(s)	281, 410, 430, 472, 656, 1389
RsaNI	GTAC	6 cut(s)	280, 409, 429, 471, 655, 1388
RseI	CAYNNNNRTG	2 cut(s)	789, 1439
SaqAI	TTAA	5 cut(s)	257, 510, 666, 1218, 1257
Sau3AI	GATC	4 cut(s)	760, 1056, 1270, 1473
Sau96I	GGNCC	4 cut(s)	520, 920, 1406, 1463
ScaI	AGTACT	1 cut(s)	281
SchI	GAGTC	1 cut(s)	1102
ScrFI	CCNGG	7 cut(s)	539, 567, 980, 1235, 1459, 1460, 1478
SduI	GDGCHC	1 cut(s)	1082
SfaNI	GCATC	4 cut(s)	118, 262, 1156, 1194
SfcI	CTRYAG	2 cut(s)	72, 147
SinI	GGWCC	4 cut(s)	520, 920, 1406, 1463
SmaI	CCCGGG	1 cut(s)	1460
SmiMI	CAYNNNNRTG	2 cut(s)	789, 1439
SmlI	CTYRAG	2 cut(s)	1217, 1391
SmoI	CTYRAG	2 cut(s)	1217, 1391
SpeI	ACTAGT	2 cut(s)	557, 1436
Sse9I	AATT	4 cut(s)	254, 542, 888, 1068
SseBI	AGGCCT	1 cut(s)	609
SsiI	CCGC	4 cut(s)	139, 152, 712, 1367
SspMI	CTAG	2 cut(s)	558, 1437
StuI	AGGCCT	1 cut(s)	609
StyD4I	CCNGG	7 cut(s)	537, 565, 978, 1233, 1457, 1458, 1476
StyI	CCWWGG	2 cut(s)	16, 610
TaaI	ACNGT	5 cut(s)	325, 931, 1005, 1139, 1510
TaiI	ACGT	2 cut(s)	1318, 1488
TaqI	TCGA	5 cut(s)	44, 188, 333, 365, 425
TasI	AATT	4 cut(s)	254, 542, 888, 1068
TatI	WGTACW	4 cut(s)	279, 408, 428, 1387
TauI	GCSGC	1 cut(s)	142
TfiI	GAWTC	2 cut(s)	896, 1225
Tru1I	TTAA	5 cut(s)	257, 510, 666, 1218, 1257
Tru9I	TTAA	5 cut(s)	257, 510, 666, 1218, 1257
TscAI	CASTG	9 cut(s)	91, 328, 343, 654, 696, 936, 970, 1424, 1513
TseFI	GTSAC	1 cut(s)	86
TseI	GCWGC	9 cut(s)	74, 146, 149, 342, 584, 719, 722, 1027, 1550
Tsp45I	GTSAC	1 cut(s)	86
TspDTI	ATGAA	4 cut(s)	722, 1151, 1161, 1480
TspGWI	ACGGA	1 cut(s)	351
TspMI	CCCGGG	1 cut(s)	1458
TspRI	CASTG	9 cut(s)	91, 328, 343, 654, 696, 936, 970, 1424, 1513
Vha464I	CTTAAG	1 cut(s)	1217
VneI	GTGCAC	1 cut(s)	1078
VpaK11BI	GGWCC	4 cut(s)	520, 920, 1406, 1463
XapI	RAATTY	1 cut(s)	542
XcmI	CCANNNNNNNNNTGG	2 cut(s)	470, 1424
XmaI	CCCGGG	1 cut(s)	1458
XmnI	GAANNNNTTC	1 cut(s)	1147
XspI	CTAG	2 cut(s)	558, 1437
ZraI	GACGTC	1 cut(s)	1486
ZrmI	AGTACT	1 cut(s)	281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.