Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012724

Physical Location & Seq

Genomic Coordinates Reverse (-)

46750159 .. 46753863

Gene Structure 3705 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1349700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGGGAACCAGCTTCAAAGCACTTCTGTTCCTTACAAGCCTGTTGTTTTCTCTTGTATCCTCTGCACCCAATGATGGGTTGTCCAGGATTGGACTGAAAAAGGTGAAACTGGATTCGAACGACTGGGTTGCTGCGCAGCTAGAATCCAAGAATGGATTGAGAAAGTATCGTCTCCCTTCGAATCTCGGAGAACCCGAGTCCGGGGATGTCGACATTGTTTCGTTAAAGAACTACATGGATGCTCAGTACTATGGTGAGATTGCCATTGGCACCCCCCCTCAACCCTTCACTGTCATTTTTGACACTGGAAGCTCGAACTTGTGGGTTCCATCGTCCAAGTGTTATGTCTCGCTTGCATGCTTCTTCCATGCCAAGTACAACTCGGGCCAATCGAGAACATACAAGAACAACGGGACTTCTGCTTCCATCCAATATGGTAGCGGAGCTATTTCAGGCTTCTTCAGCAATGACAATGTTAAAGTCGGGGACATCGTTGTTAAGAACCAGGATTTCATCGAAGCAACTAGTGAACCCGGTCTCACATTCGTGACCGCCAAGTTTGATGGCTTATTGGGACTTGGGTTTCAAGAGATTTCGGTTGGAGGTTCTGTCCCAGTGTGGTACAACATGGTTGAGCGAGGTCTTATCAAGGAACCGGTGTTTTCCTTTTGGCTCAATCGCAACGCACAAGATGAAGCAGGTGGTGAAATTGTGTTTGGCGGAGTTGATCCGAAACACTTTAAGGGCAAGCACACATATGTTCCAGTAACGAGGAAAGGCTATTGGCAGTTCAACATGGGTGATGTTCTTATTGGTGGTAAACCAACTGGATTTTGTGCTGGTGGCTGTTTCGCAATTGCAGACTCTGGAACTTCTCTTTTGACAGGTCCATCGACTTCAATTACGAAGATAAATCAAGCCATTGGAGCCAGCGGTGTTGTTAGCCAGGAATGCAAGGCGGTTGTTAACCAGTATGGGCGAACCATTCTTGATTTACTCGTAGCTCATGAGGTCCAACCAAAGAAGGTTTGTTCTCAAATTGGAGTGTGCACCTTTGACGGAACTCGTAGTGTAAGCATGGGCATTGAGAGCGTGGTGGATGAGAAAAACGGCAAATCTTCTGGCATTCTTCATGATGCTTCATGCTCTGCGTGTGAAATGGCAGTTGTCTGGATGCAAAACGAGCTTAAGAAGAATATTACGCAAGATCGTATTTTGAACTACGTCAATGAGCTTTGTGAAAAAATGCCAGACACATTGGGACAATCTACTGTCGACTGTGGAAAGCTTTCTTCCATGCCTCCCGTCTCCTTCACCATTGGCGGTAGGGCTTTTAAGCTCACTTCAGATGAGTACATACTTAAGGTCGGCGAGGGTGCTCAAGCTCAGTGCATAAGTGGATTCACTAGCCTGGATATCCCTCGTCCCCGCGGACCTCTCTGGATCTTGGGAGATATATTCATGGGTCGTTACCACACCGTGTTCGATTATGGCAAGATGAGAGTCGGATTTGCAGATGCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0010035 GO:0010038 GO:0016787 GO:0019538 GO:0030163 GO:0042221 GO:0043170 GO:0044238 GO:0046686 GO:0050896 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

509

Amino Acids

54.87

Weight (kDa)

6.61

Isoelectric Point (pI)

31.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	82 - 506	2.8e-113	Eukaryotic aspartyl protease
TAXi_N	PF14543	83 - 241	9.5e-12	Xylanase inhibitor N-terminal
SapB_2	PF03489	316 - 350	1.3e-11	Saposin-like type B, region 2
SapB_1	PF05184	381 - 417	5.6e-13	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04460 AT4G04460
fragaria_vesca	FvH4_3g01710
malus_domestica	MD05G1349700.v1.1 MD10G1324400.v1.1
prunus_persica	Prupe.4G017700_v2.0.a1 Prupe.4G017700_v2.0.a1
pyrus_communis	pycom05g31730 pycom10g27480
rosa_chinensis	RchiOBHm_Chr5g0002571
rosa_laevigata	RLG00000031040
rosa_roxburghii	Rroxscaffold_1G00073340
rosa_rugosa	Rorug04G0397000
rosa_samantha	Rh5AG020400 Rh5BG023100 Rh5CG022400 Rh5DG021500
rosa_wichuraiana	Rw5G001880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	689
Acc16I	TGCGCA	1 cut(s)	135
Acc36I	ACCTGC	1 cut(s)	689
AccB1I	GGYRCC	1 cut(s)	269
AccI	GTMKAC	2 cut(s)	210, 1275
AccII	CGCG	1 cut(s)	1431
AciI	CCGC	8 cut(s)	441, 552, 720, 933, 959, 1323, 1429, 1431
AclWI	GGATC	2 cut(s)	722, 1451
AcuI	CTGAAG	2 cut(s)	445, 1329
AdeI	CACNNNGTG	1 cut(s)	1480
AfaI	GTAC	4 cut(s)	248, 377, 623, 1355
AfiI	CCNNNNNNNGG	4 cut(s)	74, 75, 200, 201
AflII	CTTAAG	2 cut(s)	1187, 1361
AgeI	ACCGGT	1 cut(s)	655
AgsI	TTSAA	5 cut(s)	16, 587, 793, 900, 1219
AhlI	ACTAGT	1 cut(s)	524
AjnI	CCWGG	4 cut(s)	83, 504, 945, 1410
AjuI	GAANNNNNNNTTGG	2 cut(s)	699, 731
AleI	CACNNNNGTG	1 cut(s)	545
Alw21I	GWGCWC	2 cut(s)	1052, 1381
Alw26I	GTCTC	4 cut(s)	176, 352, 542, 1312
Alw44I	GTGCAC	1 cut(s)	1048
AlwI	GGATC	2 cut(s)	722, 1451
AlwNI	CAGNNNCTG	2 cut(s)	866, 1520
Ama87I	CYCGRG	2 cut(s)	194, 382
AoxI	GGCC	1 cut(s)	385
ApaLI	GTGCAC	1 cut(s)	1048
ApeKI	GCWGC	3 cut(s)	131, 136, 1520
AsiGI	ACCGGT	1 cut(s)	655
Asp700I	GAANNNNTTC	1 cut(s)	1288
AspLEI	GCGC	1 cut(s)	136
AspS9I	GGNCC	4 cut(s)	385, 887, 1012, 1433
AsuC2I	CCSGG	2 cut(s)	202, 534
AsuHPI	GGTGA	5 cut(s)	115, 266, 716, 812, 1306
AsuII	TTCGAA	2 cut(s)	116, 179
AvaI	CYCGRG	2 cut(s)	194, 382
AvaII	GGWCC	3 cut(s)	887, 1012, 1433
BaeGI	GKGCMC	1 cut(s)	1052
BanI	GGYRCC	1 cut(s)	269
Bbv12I	GWGCWC	2 cut(s)	1052, 1381
BbvI	GCAGC	3 cut(s)	118, 148, 1507
BccI	CCATC	5 cut(s)	68, 337, 434, 557, 898
BceAI	ACGGC	1 cut(s)	1126
BcgI	CGANNNNNNTGC	2 cut(s)	110, 144
BciT130I	CCWGG	4 cut(s)	85, 506, 947, 1412
BciVI	GTATCC	1 cut(s)	67
BcnI	CCSGG	2 cut(s)	202, 534
BcoDI	GTCTC	4 cut(s)	176, 352, 542, 1312
BcuI	ACTAGT	1 cut(s)	524
BfaI	CTAG	3 cut(s)	140, 525, 1407
BfrI	CTTAAG	2 cut(s)	1187, 1361
BfuAI	ACCTGC	1 cut(s)	689
BfuI	GTATCC	1 cut(s)	67
BisI	GCNGC	3 cut(s)	132, 137, 1521
BlsI	GCNGC	3 cut(s)	133, 138, 1522
BmcAI	AGTACT	1 cut(s)	248
Bme1390I	CCNGG	6 cut(s)	85, 202, 506, 534, 947, 1412
Bme18I	GGWCC	3 cut(s)	887, 1012, 1433
BmeT110I	CYCGRG	2 cut(s)	194, 382
BmgT120I	GGNCC	4 cut(s)	385, 887, 1012, 1433
BmiI	GGNNCC	5 cut(s)	7, 271, 327, 654, 928
BmrFI	CCNGG	6 cut(s)	85, 202, 506, 534, 947, 1412
BmrI	ACTGGG	2 cut(s)	133, 608
BmsI	GCATC	4 cut(s)	229, 1126, 1164, 1507
BmuI	ACTGGG	2 cut(s)	133, 608
Bpu14I	TTCGAA	2 cut(s)	116, 179
BpuEI	CTTGAG	1 cut(s)	1365
BpuMI	CCSGG	2 cut(s)	202, 534
BsaI	GGTCTC	1 cut(s)	542
BsaJI	CCNNGG	2 cut(s)	201, 1429
BsaWI	WCCGGW	1 cut(s)	655
BsaXI	ACNNNNNCTCC	2 cut(s)	594, 624
Bsc4I	CCNNNNNNNGG	4 cut(s)	74, 75, 200, 201
Bse118I	RCCGGY	1 cut(s)	655
Bse1I	ACTGG	7 cut(s)	114, 128, 310, 614, 764, 832, 970
Bse3DI	GCAATG	1 cut(s)	472
BseBI	CCWGG	4 cut(s)	85, 506, 947, 1412
BseDI	CCNNGG	2 cut(s)	201, 1429
BseGI	GGATG	5 cut(s)	211, 244, 426, 1105, 1179
BseLI	CCNNNNNNNGG	4 cut(s)	74, 75, 200, 201
BseMI	GCAATG	1 cut(s)	472
BseMII	CTCAG	2 cut(s)	257, 1400
BseNI	ACTGG	7 cut(s)	114, 128, 310, 614, 764, 832, 970
BseSI	GKGCMC	1 cut(s)	1052
BseXI	GCAGC	3 cut(s)	118, 148, 1507
BsgI	GTGCAG	1 cut(s)	48
Bsh1236I	CGCG	1 cut(s)	1431
BshFI	GGCC	1 cut(s)	387
BshNI	GGYRCC	1 cut(s)	269
BshTI	ACCGGT	1 cut(s)	655
BsiHKAI	GWGCWC	2 cut(s)	1052, 1381
BsiHKCI	CYCGRG	2 cut(s)	194, 382
BsiSI	CCGG	3 cut(s)	201, 534, 656
BslFI	GGGAC	6 cut(s)	427, 500, 588, 596, 1275, 1410
BslI	CCNNNNNNNGG	4 cut(s)	74, 75, 200, 201
BsmAI	GTCTC	4 cut(s)	176, 352, 542, 1312
BsmBI	CGTCTC	2 cut(s)	176, 1312
BsmFI	GGGAC	6 cut(s)	427, 500, 588, 596, 1275, 1410
BsmI	GAATGC	2 cut(s)	956, 1125
BsnI	GGCC	1 cut(s)	387
Bso31I	GGTCTC	1 cut(s)	542
BsoBI	CYCGRG	2 cut(s)	194, 382
Bsp119I	TTCGAA	2 cut(s)	116, 179
Bsp1286I	GDGCHC	2 cut(s)	1052, 1381
Bsp143I	GATC	3 cut(s)	727, 1207, 1443
BspACI	CCGC	8 cut(s)	441, 552, 720, 933, 959, 1323, 1429, 1431
BspANI	GGCC	1 cut(s)	387
BspCNI	CTCAG	2 cut(s)	256, 1399
BspFNI	CGCG	1 cut(s)	1431
BspHI	TCATGA	2 cut(s)	1006, 1132
BspLI	GGNNCC	5 cut(s)	7, 271, 327, 654, 928
BspMI	ACCTGC	1 cut(s)	689
BspPI	GGATC	2 cut(s)	722, 1451
BspT104I	TTCGAA	2 cut(s)	116, 179
BspT107I	GGYRCC	1 cut(s)	269
BspTI	CTTAAG	2 cut(s)	1187, 1361
BspTNI	GGTCTC	1 cut(s)	542
BsrDI	GCAATG	1 cut(s)	472
BsrFI	RCCGGY	1 cut(s)	655
BsrI	ACTGG	7 cut(s)	114, 128, 310, 614, 764, 832, 970
BssAI	RCCGGY	1 cut(s)	655
BssECI	CCNNGG	2 cut(s)	201, 1429
BssMI	GATC	3 cut(s)	727, 1207, 1443
Bst2UI	CCWGG	4 cut(s)	85, 506, 947, 1412
Bst4CI	ACNGT	4 cut(s)	292, 1273, 1280, 1480
BstAFI	CTTAAG	2 cut(s)	1187, 1361
BstAPI	GCANNNNNTGC	1 cut(s)	1520
BstBI	TTCGAA	2 cut(s)	116, 179
BstC8I	GCNNGC	4 cut(s)	354, 358, 749, 931
BstDEI	CTNAG	2 cut(s)	243, 1386
BstDSI	CCRYGG	1 cut(s)	1429
BstF5I	GGATG	5 cut(s)	211, 244, 426, 1105, 1179
BstFNI	CGCG	1 cut(s)	1431
BstHHI	GCGC	1 cut(s)	136
BstKTI	GATC	3 cut(s)	730, 1210, 1446
BstMAI	GTCTC	4 cut(s)	176, 352, 542, 1312
BstMBI	GATC	3 cut(s)	727, 1207, 1443
BstMWI	GCNNNNNNNGC	6 cut(s)	462, 926, 951, 1089, 1183, 1520
BstNI	CCWGG	4 cut(s)	85, 506, 947, 1412
BstNSI	RCATGY	1 cut(s)	360
BstSCI	CCNGG	6 cut(s)	83, 200, 504, 532, 945, 1410
BstSLI	GKGCMC	1 cut(s)	1052
BstUI	CGCG	1 cut(s)	1431
BstV1I	GCAGC	3 cut(s)	118, 148, 1507
BstX2I	RGATCY	1 cut(s)	1443
BstYI	RGATCY	1 cut(s)	1443
BsuI	GTATCC	1 cut(s)	67
BsuRI	GGCC	1 cut(s)	387
BtgI	CCRYGG	1 cut(s)	1429
BtsCI	GGATG	5 cut(s)	211, 244, 426, 1105, 1179
BtsIMutI	CAGTG	4 cut(s)	288, 303, 621, 1394
BveI	ACCTGC	1 cut(s)	689
Cac8I	GCNNGC	4 cut(s)	354, 358, 749, 931
CaiI	CAGNNNCTG	2 cut(s)	866, 1520
CciI	TCATGA	2 cut(s)	1006, 1132
CfoI	GCGC	1 cut(s)	136
Cfr10I	RCCGGY	1 cut(s)	655
Cfr13I	GGNCC	4 cut(s)	385, 887, 1012, 1433
Cfr42I	CCGCGG	1 cut(s)	1432
Csp6I	GTAC	4 cut(s)	247, 376, 622, 1354
CspAI	ACCGGT	1 cut(s)	655
CviQI	GTAC	4 cut(s)	247, 376, 622, 1354
DdeI	CTNAG	2 cut(s)	243, 1386
DpnI	GATC	3 cut(s)	729, 1209, 1445
DpnII	GATC	3 cut(s)	727, 1207, 1443
DraIII	CACNNNGTG	1 cut(s)	1480
EciI	GGCGGA	1 cut(s)	735
Eco31I	GGTCTC	1 cut(s)	542
Eco32I	GATATC	1 cut(s)	1417
Eco47I	GGWCC	3 cut(s)	887, 1012, 1433
Eco57I	CTGAAG	2 cut(s)	445, 1329
Eco88I	CYCGRG	2 cut(s)	194, 382
EcoRII	CCWGG	4 cut(s)	83, 504, 945, 1410
EcoRV	GATATC	1 cut(s)	1417
Esp3I	CGTCTC	2 cut(s)	176, 1312
FaqI	GGGAC	6 cut(s)	427, 500, 588, 596, 1275, 1410
FauI	CCCGC	1 cut(s)	1436
FauNDI	CATATG	1 cut(s)	757
FblI	GTMKAC	2 cut(s)	210, 1275
Fnu4HI	GCNGC	3 cut(s)	132, 137, 1521
FokI	GGATG	5 cut(s)	218, 251, 413, 1112, 1186
Fsp4HI	GCNGC	3 cut(s)	132, 137, 1521
FspBI	CTAG	3 cut(s)	140, 525, 1407
FspI	TGCGCA	1 cut(s)	135
GlaI	GCGC	1 cut(s)	135
GluI	GCNGC	3 cut(s)	132, 137, 1521
HaeIII	GGCC	1 cut(s)	387
HapII	CCGG	3 cut(s)	201, 534, 656
HhaI	GCGC	1 cut(s)	136
Hin6I	GCGC	1 cut(s)	134
HinP1I	GCGC	1 cut(s)	134
HincII	GTYRAC	3 cut(s)	211, 967, 1276
HindII	GTYRAC	3 cut(s)	211, 967, 1276
HindIII	AAGCTT	1 cut(s)	1286
HinfI	GANTC	7 cut(s)	113, 143, 181, 197, 863, 1401, 1503
HpaI	GTTAAC	1 cut(s)	967
HpaII	CCGG	3 cut(s)	201, 534, 656
HphI	GGTGA	5 cut(s)	115, 266, 716, 812, 1306
Hpy166II	GTNNAC	6 cut(s)	211, 530, 821, 967, 1050, 1276
Hpy188I	TCNGA	4 cut(s)	188, 732, 1348, 1508
Hpy188III	TCNNGA	9 cut(s)	393, 547, 587, 867, 989, 1007, 1133, 1171, 1441
Hpy8I	GTNNAC	6 cut(s)	211, 530, 821, 967, 1050, 1276
HpyAV	CCTTC	4 cut(s)	186, 295, 1018, 1321
HpyCH4III	ACNGT	4 cut(s)	292, 1273, 1280, 1480
HpyCH4IV	ACGT	1 cut(s)	1224
HpyCH4V	TGCA	9 cut(s)	65, 356, 860, 954, 1050, 1177, 1392, 1514, 1523
HpyF10VI	GCNNNNNNNGC	6 cut(s)	462, 926, 951, 1089, 1183, 1520
HpyF3I	CTNAG	2 cut(s)	243, 1386
HpySE526I	ACGT	1 cut(s)	1224
HspAI	GCGC	1 cut(s)	134
KspAI	GTTAAC	1 cut(s)	967
KspI	CCGCGG	1 cut(s)	1432
Kzo9I	GATC	3 cut(s)	727, 1207, 1443
LmnI	GCTCC	2 cut(s)	443, 926
Lsp1109I	GCAGC	3 cut(s)	118, 148, 1507
LweI	GCATC	4 cut(s)	229, 1126, 1164, 1507
MaeI	CTAG	3 cut(s)	140, 525, 1407
MaeII	ACGT	1 cut(s)	1224
MaeIII	GTNAC	3 cut(s)	547, 766, 1469
MalI	GATC	3 cut(s)	729, 1209, 1445
MboI	GATC	3 cut(s)	727, 1207, 1443
MboII	GAAGA	7 cut(s)	355, 451, 919, 1110, 1121, 1204, 1284
MfeI	CAATTG	1 cut(s)	855
MflI	RGATCY	1 cut(s)	1443
MhlI	GDGCHC	2 cut(s)	1052, 1381
MluCI	AATT	4 cut(s)	708, 855, 900, 1038
MlyI	GAGTC	3 cut(s)	206, 857, 1512
MmeI	TCCRAC	3 cut(s)	580, 1039, 1486
MroXI	GAANNNNTTC	1 cut(s)	1288
MseI	TTAA	8 cut(s)	224, 477, 498, 741, 966, 1188, 1335, 1362
MslI	CAYNNNNRTG	2 cut(s)	545, 756
MspA1I	CMGCKG	2 cut(s)	933, 1431
MspCI	CTTAAG	2 cut(s)	1187, 1361
MspI	CCGG	3 cut(s)	201, 534, 656
MspR9I	CCNGG	6 cut(s)	85, 202, 506, 534, 947, 1412
MunI	CAATTG	1 cut(s)	855
Mva1269I	GAATGC	2 cut(s)	956, 1125
MvaI	CCWGG	4 cut(s)	85, 506, 947, 1412
MvnI	CGCG	1 cut(s)	1431
MwoI	GCNNNNNNNGC	6 cut(s)	462, 926, 951, 1089, 1183, 1520
NciI	CCSGG	2 cut(s)	202, 534
NdeI	CATATG	1 cut(s)	757
NdeII	GATC	3 cut(s)	727, 1207, 1443
NlaIV	GGNNCC	5 cut(s)	7, 271, 327, 654, 928
NmuCI	GTSAC	1 cut(s)	547
NsbI	TGCGCA	1 cut(s)	135
NspI	RCATGY	1 cut(s)	360
NspV	TTCGAA	2 cut(s)	116, 179
OliI	CACNNNNGTG	1 cut(s)	545
PaeI	GCATGC	1 cut(s)	360
PagI	TCATGA	2 cut(s)	1006, 1132
PaqCI	CACCTGC	1 cut(s)	689
PcsI	WCGNNNNNNNCGW	3 cut(s)	192, 389, 489
PctI	GAATGC	2 cut(s)	956, 1125
PdmI	GAANNNNTTC	1 cut(s)	1288
PfeI	GAWTC	4 cut(s)	113, 143, 181, 1401
PfoI	TCCNGGA	1 cut(s)	83
PinAI	ACCGGT	1 cut(s)	655
PkrI	GCNGC	3 cut(s)	133, 138, 1522
PleI	GAGTC	3 cut(s)	205, 857, 1511
PpsI	GAGTC	3 cut(s)	205, 857, 1511
Psp6I	CCWGG	4 cut(s)	83, 504, 945, 1410
PspGI	CCWGG	4 cut(s)	83, 504, 945, 1410
PspN4I	GGNNCC	5 cut(s)	7, 271, 327, 654, 928
PspPI	GGNCC	4 cut(s)	385, 887, 1012, 1433
PstNI	CAGNNNCTG	2 cut(s)	866, 1520
PsuI	RGATCY	1 cut(s)	1443
RsaI	GTAC	4 cut(s)	248, 377, 623, 1355
RsaNI	GTAC	4 cut(s)	247, 376, 622, 1354
RseI	CAYNNNNRTG	2 cut(s)	545, 756
SacII	CCGCGG	1 cut(s)	1432
SalI	GTCGAC	2 cut(s)	209, 1274
SaqAI	TTAA	8 cut(s)	224, 477, 498, 741, 966, 1188, 1335, 1362
SatI	GCNGC	3 cut(s)	132, 137, 1521
Sau3AI	GATC	3 cut(s)	727, 1207, 1443
Sau96I	GGNCC	4 cut(s)	385, 887, 1012, 1433
ScaI	AGTACT	1 cut(s)	248
SchI	GAGTC	3 cut(s)	206, 857, 1512
ScrFI	CCNGG	6 cut(s)	85, 202, 506, 534, 947, 1412
SduI	GDGCHC	2 cut(s)	1052, 1381
SfaNI	GCATC	4 cut(s)	229, 1126, 1164, 1507
Sfr303I	CCGCGG	1 cut(s)	1432
SfuI	TTCGAA	2 cut(s)	116, 179
SgrBI	CCGCGG	1 cut(s)	1432
SinI	GGWCC	3 cut(s)	887, 1012, 1433
SmiMI	CAYNNNNRTG	2 cut(s)	545, 756
SmlI	CTYRAG	3 cut(s)	1187, 1361, 1380
SmoI	CTYRAG	3 cut(s)	1187, 1361, 1380
SpeI	ACTAGT	1 cut(s)	524
SphI	GCATGC	1 cut(s)	360
Sse9I	AATT	4 cut(s)	708, 855, 900, 1038
SsiI	CCGC	8 cut(s)	441, 552, 720, 933, 959, 1323, 1429, 1431
SspI	AATATT	1 cut(s)	1198
SspMI	CTAG	3 cut(s)	140, 525, 1407
StyD4I	CCNGG	6 cut(s)	83, 200, 504, 532, 945, 1410
TaaI	ACNGT	4 cut(s)	292, 1273, 1280, 1480
TaiI	ACGT	1 cut(s)	1227
TaqI	TCGA	9 cut(s)	116, 179, 210, 314, 392, 516, 893, 1275, 1485
TasI	AATT	4 cut(s)	708, 855, 900, 1038
TatI	WGTACW	3 cut(s)	246, 375, 1353
TfiI	GAWTC	4 cut(s)	113, 143, 181, 1401
Tru1I	TTAA	8 cut(s)	224, 477, 498, 741, 966, 1188, 1335, 1362
Tru9I	TTAA	8 cut(s)	224, 477, 498, 741, 966, 1188, 1335, 1362
TscAI	CASTG	4 cut(s)	295, 310, 621, 1394
TseFI	GTSAC	1 cut(s)	547
TseI	GCWGC	3 cut(s)	131, 136, 1520
Tsp45I	GTSAC	1 cut(s)	547
TspDTI	ATGAA	5 cut(s)	502, 708, 1121, 1131, 1450
TspGWI	ACGGA	1 cut(s)	1074
TspRI	CASTG	4 cut(s)	295, 310, 621, 1394
Vha464I	CTTAAG	2 cut(s)	1187, 1361
VneI	GTGCAC	1 cut(s)	1048
VpaK11BI	GGWCC	3 cut(s)	887, 1012, 1433
XceI	RCATGY	1 cut(s)	360
XmiI	GTMKAC	2 cut(s)	210, 1275
XmnI	GAANNNNTTC	1 cut(s)	1288
XspI	CTAG	3 cut(s)	140, 525, 1407
ZrmI	AGTACT	1 cut(s)	248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.