Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0012724

Physical Location & Seq

Genomic Coordinates Forward (+)

1521712 .. 1526738

Gene Structure 5027 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031040

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGAAAATGAAGTATTTTGACAAACGAGAGTTATTATGCTTCTGTTTTAATGGACATGAAACCTTGATCAAGGTAATTATGGGAACCAGCTTCCAAGGGGTTGTGTTGTCTCTGTTCGTTTCGAGCTTGTTGTTGTGTCTGGTGTCCTCTGCAGCCCCACCCAGTGATGGTTTGTTTAGGATTGGACTGAAAAAGGTGAAGTTGGATGCGAATGAGCGGCTTGCTGCAGCGGTTGAATACAGGAATGCAGAGGATTTGAGGGGTTCGATTAGGAAGTATGGTTTCCCTTCTAATCTGGGAGACGCTGAGGGGGAGGATATTGATATTGTCAGATTAAAGAACTACTTGGATGCTCAGTACTATGGTGAGATAGCTATTGGCACTCCCCCTCAGCCGTTCACTGTGATTTTCGACACTGGCAGCTCTAATCTGTGGGTTCCGTCGAGCAACTGTTATTTCTCGGTTGCTTGCTACTTCCATGCCAAGTACAAGTCAAGCCAATCGAGTACATACAAGAAGAATGGGAAATCTGCTTCCATACAGTATGGTACTGGAGCTATTTCAGGTTTCTTTAGTATTGACAATGTTAAAGTTGGGGACCTCGTCGTGAAAGACCAGGAATTTATCGAAGCAACTAGTGAGCCTGGTCTAACATTTTTGGCTGCCAAGTTTGATGGGCTATTAGGCCTTGGATTTCAAGAGATTTCAGTTGGAAATGCTGTCCCAGTGTGGTACAACATGATTAAGCAAGGGCTTATCAAGGAACCAGTGTTTTCTTTTTGGATGAACCGCAATGCAGCAGCACAAGAAGGTGGTGAACTTGTGTTTGGGGGAGTTGATCCCCATCACTTCAAGGGAAAGCACACATATGTTCCTGTAACACAGAAAGGCTATTGGCAGTTCGACATGGGTGATGTTCTTATTGGTGATAAACCAACTGGATATTGTGCTGGTGGATGTCATGCAATTGCAGATTCTGGAACTTCTCTTTTGGCTGGTCCAACGACAGTGGTTACATTGATAAATAAAGCCATTGGAGCCAATGGGGTTGTTAGCCAGGAATGCAAGGCTGTTGTTCAACAGTATGGTCAAACCATTATGGAGCTGCTCTTGGCTGAGGCTCAACCAAAGAAGATCTGCTCCCAAATTGGAGTGTGCACCTTTGATGGGAGTCATAGTGTTAGTATGGGCATTGAGAGTGTGGTGGATAAGAACCGTGGAAAATCTTCTGGAGTTCTTCATGATGCTTCATGCTCTGTTTGTGAGATGGCAGTTGTCTGGATGCAAAACCAGCTTAAGCAGAATCAGACCCAGGATTATATTTTGAACTATGTTAATGAGCTTTGTGATCAAATGCCAAGCCCAAATGGACAATCTTCTGTTGACTGCCAACGTCTTTCTTCCATGCCTACCATTTCCTTCACCATTGGTGGTAAAGTTTTTCCGCTCACCCCAGAGGAGTATGTACTCAAGGTGGGCGAGGGTCCACAAGCCCAGTGCATAAGTGGCTTCACTAGTATGGACATTCCTCCTCCCCGGGGACCTCTCTGGATCCTGGGAGACGTCTTCATGGGTCCCTACCACACTGTCTTTGATTATGGTAATATGAGAGTTGGATTTGCAAAGGCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0010035 GO:0010038 GO:0016787 GO:0019538 GO:0030163 GO:0042221 GO:0043170 GO:0044238 GO:0046686 GO:0050896 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

545

Amino Acids

59.13

Weight (kDa)

5.9

Isoelectric Point (pI)

32.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	119 - 543	7.4e-117	Eukaryotic aspartyl protease
TAXi_N	PF14543	120 - 278	1.5e-11	Xylanase inhibitor N-terminal
SapB_2	PF03489	353 - 386	1.4e-11	Saposin-like type B, region 2
SapB_1	PF05184	417 - 453	9e-14	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0012724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04460 AT4G04460
fragaria_vesca	FvH4_3g01710
malus_domestica	MD05G1349700.v1.1 MD10G1324400.v1.1
prunus_persica	Prupe.4G017700_v2.0.a1 Prupe.4G017700_v2.0.a1
pyrus_communis	pycom05g31730 pycom10g27480
rosa_chinensis	RchiOBHm_Chr5g0002571
rosa_laevigata	RLG00000031040
rosa_roxburghii	Rroxscaffold_1G00073340
rosa_rugosa	Rorug04G0397000
rosa_samantha	Rh5AG020400 Rh5BG023100 Rh5CG022400 Rh5DG021500
rosa_wichuraiana	Rw5G001880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1566
AccBSI	CCGCTC	2 cut(s)	217, 1447
AciI	CCGC	4 cut(s)	217, 230, 790, 1445
AclWI	GGATC	3 cut(s)	833, 1546, 1559
AcsI	RAATTY	1 cut(s)	620
AcyI	GRCGYC	1 cut(s)	1563
AdeI	CACNNNGTG	1 cut(s)	164
AfaI	GTAC	6 cut(s)	359, 488, 508, 550, 734, 1467
AfiI	CCNNNNNNNGG	3 cut(s)	167, 1167, 1538
AflII	CTTAAG	1 cut(s)	1295
AgsI	TTSAA	5 cut(s)	236, 698, 853, 1079, 1327
AhlI	ACTAGT	2 cut(s)	635, 1514
AjnI	CCWGG	5 cut(s)	615, 643, 1056, 1311, 1554
AjuI	GAANNNNNNNTTGG	2 cut(s)	810, 842
AluBI	AGCT	8 cut(s)	90, 126, 374, 423, 557, 1105, 1294, 1342
AluI	AGCT	8 cut(s)	90, 126, 374, 423, 557, 1105, 1294, 1342
Alw21I	GWGCWC	1 cut(s)	1160
Alw26I	GTCTC	3 cut(s)	114, 294, 1554
Alw44I	GTGCAC	1 cut(s)	1156
AlwI	GGATC	3 cut(s)	833, 1546, 1559
AlwNI	CAGNNNCTG	1 cut(s)	977
Ama87I	CYCGRG	1 cut(s)	1536
AoxI	GGCC	1 cut(s)	685
ApaLI	GTGCAC	1 cut(s)	1156
ApeKI	GCWGC	9 cut(s)	152, 224, 227, 420, 662, 797, 800, 1105, 1628
ApoI	RAATTY	1 cut(s)	620
Asp700I	GAANNNNTTC	1 cut(s)	1225
AspS9I	GGNCC	5 cut(s)	598, 998, 1484, 1541, 1574
AsuC2I	CCSGG	2 cut(s)	1537, 1538
AsuHPI	GGTGA	7 cut(s)	208, 377, 827, 923, 938, 1414, 1441
AvaI	CYCGRG	1 cut(s)	1536
AvaII	GGWCC	5 cut(s)	598, 998, 1484, 1541, 1574
BaeGI	GKGCMC	1 cut(s)	1160
BamHI	GGATCC	1 cut(s)	1551
BbsI	GAAGAC	1 cut(s)	1558
Bbv12I	GWGCWC	1 cut(s)	1160
BbvCI	CCTCAGC	3 cut(s)	306, 390, 1116
BbvI	GCAGC	9 cut(s)	164, 211, 239, 432, 649, 809, 812, 1092, 1615
BccI	CCATC	5 cut(s)	161, 668, 852, 1160, 1261
BceAI	ACGGC	1 cut(s)	379
BciT130I	CCWGG	5 cut(s)	617, 645, 1058, 1313, 1556
BclI	TGATCA	2 cut(s)	66, 1348
BcnI	CCSGG	2 cut(s)	1537, 1538
BcoDI	GTCTC	3 cut(s)	114, 294, 1554
BcuI	ACTAGT	2 cut(s)	635, 1514
BfaI	CTAG	2 cut(s)	636, 1515
BfmI	CTRYAG	2 cut(s)	150, 225
BfrI	CTTAAG	1 cut(s)	1295
BglII	AGATCT	1 cut(s)	1134
BmcAI	AGTACT	1 cut(s)	359
Bme1390I	CCNGG	7 cut(s)	617, 645, 1058, 1313, 1537, 1538, 1556
Bme18I	GGWCC	5 cut(s)	598, 998, 1484, 1541, 1574
BmeT110I	CYCGRG	1 cut(s)	1536
BmgT120I	GGNCC	5 cut(s)	598, 998, 1484, 1541, 1574
BmrFI	CCNGG	7 cut(s)	617, 645, 1058, 1313, 1537, 1538, 1556
BmrI	ACTGGG	3 cut(s)	156, 719, 1489
BmsI	GCATC	4 cut(s)	196, 340, 1234, 1272
BmuI	ACTGGG	3 cut(s)	156, 719, 1489
BpiI	GAAGAC	1 cut(s)	1558
BpmI	CTGGAG	2 cut(s)	573, 1251
Bpu10I	CCTNAGC	3 cut(s)	306, 390, 1116
BpuEI	CTTGAG	1 cut(s)	1454
BpuMI	CCSGG	2 cut(s)	1537, 1538
BsaBI	GATNNNNATC	1 cut(s)	843
BsaHI	GRCGYC	1 cut(s)	1563
BsaJI	CCNNGG	8 cut(s)	94, 688, 1216, 1311, 1535, 1536, 1537, 1555
BsaXI	ACNNNNNCTCC	2 cut(s)	1162, 1192
Bsc4I	CCNNNNNNNGG	3 cut(s)	167, 1167, 1538
Bse1I	ACTGG	7 cut(s)	162, 421, 556, 725, 767, 943, 1495
Bse3DI	GCAATG	1 cut(s)	799
Bse8I	GATNNNNATC	1 cut(s)	843
BseBI	CCWGG	5 cut(s)	617, 645, 1058, 1313, 1556
BseDI	CCNNGG	8 cut(s)	94, 688, 1216, 1311, 1535, 1536, 1537, 1555
BseGI	GGATG	5 cut(s)	211, 355, 789, 962, 1287
BseJI	GATNNNNATC	1 cut(s)	843
BseLI	CCNNNNNNNGG	3 cut(s)	167, 1167, 1538
BseMI	GCAATG	1 cut(s)	799
BseMII	CTCAG	4 cut(s)	297, 368, 404, 1107
BseNI	ACTGG	7 cut(s)	162, 421, 556, 725, 767, 943, 1495
BseRI	GAGGAG	2 cut(s)	1472, 1521
BseSI	GKGCMC	1 cut(s)	1160
BseXI	GCAGC	9 cut(s)	164, 211, 239, 432, 649, 809, 812, 1092, 1615
BshFI	GGCC	1 cut(s)	687
BsiHKAI	GWGCWC	1 cut(s)	1160
BsiHKCI	CYCGRG	1 cut(s)	1536
BsiSI	CCGG	1 cut(s)	1537
BslFI	GGGAC	4 cut(s)	611, 707, 1554, 1560
BslI	CCNNNNNNNGG	3 cut(s)	167, 1167, 1538
BsmAI	GTCTC	3 cut(s)	114, 294, 1554
BsmBI	CGTCTC	2 cut(s)	294, 1554
BsmFI	GGGAC	4 cut(s)	611, 707, 1554, 1560
BsmI	GAATGC	2 cut(s)	250, 1067
BsnI	GGCC	1 cut(s)	687
BsoBI	CYCGRG	1 cut(s)	1536
Bsp1286I	GDGCHC	1 cut(s)	1160
Bsp143I	GATC	5 cut(s)	66, 838, 1134, 1348, 1551
BspACI	CCGC	4 cut(s)	217, 230, 790, 1445
BspANI	GGCC	1 cut(s)	687
BspCNI	CTCAG	4 cut(s)	298, 367, 403, 1108
BspHI	TCATGA	1 cut(s)	1240
BspMAI	CTGCAG	2 cut(s)	154, 229
BspPI	GGATC	3 cut(s)	833, 1546, 1559
BspTI	CTTAAG	1 cut(s)	1295
BsrBI	CCGCTC	2 cut(s)	217, 1447
BsrDI	GCAATG	1 cut(s)	799
BsrI	ACTGG	7 cut(s)	162, 421, 556, 725, 767, 943, 1495
BssECI	CCNNGG	8 cut(s)	94, 688, 1216, 1311, 1535, 1536, 1537, 1555
BssMI	GATC	5 cut(s)	66, 838, 1134, 1348, 1551
BssNI	GRCGYC	1 cut(s)	1563
BssT1I	CCWWGG	2 cut(s)	94, 688
Bst2UI	CCWGG	5 cut(s)	617, 645, 1058, 1313, 1556
Bst4CI	ACNGT	7 cut(s)	403, 452, 543, 1009, 1083, 1217, 1588
BstACI	GRCGYC	1 cut(s)	1563
BstAFI	CTTAAG	1 cut(s)	1295
BstAPI	GCANNNNNTGC	1 cut(s)	1628
BstC8I	GCNNGC	2 cut(s)	222, 469
BstDEI	CTNAG	4 cut(s)	306, 354, 390, 1116
BstDSI	CCRYGG	1 cut(s)	1216
BstF5I	GGATG	5 cut(s)	211, 355, 789, 962, 1287
BstKTI	GATC	5 cut(s)	69, 841, 1137, 1351, 1554
BstMAI	GTCTC	3 cut(s)	114, 294, 1554
BstMBI	GATC	5 cut(s)	66, 838, 1134, 1348, 1551
BstMWI	GCNNNNNNNGC	5 cut(s)	1037, 1062, 1291, 1506, 1628
BstNI	CCWGG	5 cut(s)	617, 645, 1058, 1313, 1556
BstSCI	CCNGG	7 cut(s)	615, 643, 1056, 1311, 1535, 1536, 1554
BstSFI	CTRYAG	2 cut(s)	150, 225
BstSLI	GKGCMC	1 cut(s)	1160
BstV1I	GCAGC	9 cut(s)	164, 211, 239, 432, 649, 809, 812, 1092, 1615
BstV2I	GAAGAC	1 cut(s)	1558
BstX2I	RGATCY	2 cut(s)	1134, 1551
BstYI	RGATCY	2 cut(s)	1134, 1551
BsuRI	GGCC	1 cut(s)	687
BtgI	CCRYGG	1 cut(s)	1216
BtsCI	GGATG	5 cut(s)	211, 355, 789, 962, 1287
BtsIMutI	CAGTG	8 cut(s)	169, 399, 414, 732, 774, 1014, 1502, 1584
Cac8I	GCNNGC	2 cut(s)	222, 469
CaiI	CAGNNNCTG	1 cut(s)	977
CciI	TCATGA	1 cut(s)	1240
Cfr13I	GGNCC	5 cut(s)	598, 998, 1484, 1541, 1574
Cfr9I	CCCGGG	1 cut(s)	1536
CseI	GACGC	1 cut(s)	311
Csp6I	GTAC	6 cut(s)	358, 487, 507, 549, 733, 1466
CspCI	CAANNNNNGTGG	2 cut(s)	990, 1025
CviAII	CATG	9 cut(s)	56, 479, 739, 907, 962, 1241, 1251, 1405, 1570
CviQI	GTAC	6 cut(s)	358, 487, 507, 549, 733, 1466
DdeI	CTNAG	4 cut(s)	306, 354, 390, 1116
DpnI	GATC	5 cut(s)	68, 840, 1136, 1350, 1553
DpnII	GATC	5 cut(s)	66, 838, 1134, 1348, 1551
DraIII	CACNNNGTG	1 cut(s)	164
Eco130I	CCWWGG	2 cut(s)	94, 688
Eco147I	AGGCCT	1 cut(s)	687
Eco47I	GGWCC	5 cut(s)	598, 998, 1484, 1541, 1574
Eco88I	CYCGRG	1 cut(s)	1536
EcoO109I	RGGNCCY	3 cut(s)	598, 1541, 1574
EcoRII	CCWGG	5 cut(s)	615, 643, 1056, 1311, 1554
EcoT14I	CCWWGG	2 cut(s)	94, 688
ErhI	CCWWGG	2 cut(s)	94, 688
Esp3I	CGTCTC	2 cut(s)	294, 1554
FaeI	CATG	9 cut(s)	59, 482, 742, 910, 965, 1244, 1254, 1408, 1573
FalI	AAGNNNNNCTT	2 cut(s)	329, 361
FaqI	GGGAC	4 cut(s)	611, 707, 1554, 1560
FatI	CATG	9 cut(s)	55, 478, 738, 906, 961, 1240, 1250, 1404, 1569
FauNDI	CATATG	1 cut(s)	868
FbaI	TGATCA	2 cut(s)	66, 1348
FokI	GGATG	5 cut(s)	218, 362, 796, 969, 1294
FspBI	CTAG	2 cut(s)	636, 1515
GsuI	CTGGAG	2 cut(s)	573, 1251
HaeIII	GGCC	1 cut(s)	687
HapII	CCGG	1 cut(s)	1537
HgaI	GACGC	1 cut(s)	311
Hin1I	GRCGYC	1 cut(s)	1563
Hin1II	CATG	9 cut(s)	59, 482, 742, 910, 965, 1244, 1254, 1408, 1573
HincII	GTYRAC	1 cut(s)	1384
HindII	GTYRAC	1 cut(s)	1384
HinfI	GANTC	3 cut(s)	974, 1171, 1303
HpaII	CCGG	1 cut(s)	1537
HphI	GGTGA	7 cut(s)	208, 377, 827, 923, 938, 1414, 1441
Hpy166II	GTNNAC	5 cut(s)	399, 818, 1158, 1384, 1487
Hpy188I	TCNGA	2 cut(s)	332, 1308
Hpy188III	TCNNGA	7 cut(s)	607, 698, 978, 1230, 1241, 1279, 1549
Hpy8I	GTNNAC	5 cut(s)	399, 818, 1158, 1384, 1487
Hpy99I	CGWCG	2 cut(s)	445, 608
HpyAV	CCTTC	3 cut(s)	297, 803, 1429
HpyCH4III	ACNGT	7 cut(s)	403, 452, 543, 1009, 1083, 1217, 1588
HpyCH4IV	ACGT	2 cut(s)	1393, 1563
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1037, 1062, 1291, 1506, 1628
HpyF3I	CTNAG	4 cut(s)	306, 354, 390, 1116
HpySE526I	ACGT	2 cut(s)	1393, 1563
Hsp92I	GRCGYC	1 cut(s)	1563
Hsp92II	CATG	9 cut(s)	59, 482, 742, 910, 965, 1244, 1254, 1408, 1573
KflI	GGGWCCC	1 cut(s)	1574
Ksp22I	TGATCA	2 cut(s)	66, 1348
Kzo9I	GATC	5 cut(s)	66, 838, 1134, 1348, 1551
LmnI	GCTCC	4 cut(s)	554, 1037, 1102, 1145
Lsp1109I	GCAGC	9 cut(s)	164, 211, 239, 432, 649, 809, 812, 1092, 1615
LweI	GCATC	4 cut(s)	196, 340, 1234, 1272
MaeI	CTAG	2 cut(s)	636, 1515
MaeII	ACGT	2 cut(s)	1393, 1563
MaeIII	GTNAC	2 cut(s)	877, 1012
MalI	GATC	5 cut(s)	68, 840, 1136, 1350, 1553
MbiI	CCGCTC	2 cut(s)	217, 1447
MboI	GATC	5 cut(s)	66, 838, 1134, 1348, 1551
MboII	GAAGA	7 cut(s)	529, 1144, 1218, 1229, 1368, 1392, 1558
MfeI	CAATTG	1 cut(s)	966
MflI	RGATCY	2 cut(s)	1134, 1551
MhlI	GDGCHC	1 cut(s)	1160
MluCI	AATT	4 cut(s)	75, 620, 966, 1146
MlyI	GAGTC	1 cut(s)	1180
MmeI	TCCRAC	4 cut(s)	183, 691, 1025, 1594
MroXI	GAANNNNTTC	1 cut(s)	1225
MseI	TTAA	6 cut(s)	48, 335, 588, 744, 1296, 1335
MslI	CAYNNNNRTG	2 cut(s)	867, 1517
MspA1I	CMGCKG	1 cut(s)	230
MspCI	CTTAAG	1 cut(s)	1295
MspI	CCGG	1 cut(s)	1537
MspR9I	CCNGG	7 cut(s)	617, 645, 1058, 1313, 1537, 1538, 1556
MunI	CAATTG	1 cut(s)	966
Mva1269I	GAATGC	2 cut(s)	250, 1067
MvaI	CCWGG	5 cut(s)	617, 645, 1058, 1313, 1556
MwoI	GCNNNNNNNGC	5 cut(s)	1037, 1062, 1291, 1506, 1628
NciI	CCSGG	2 cut(s)	1537, 1538
NdeI	CATATG	1 cut(s)	868
NdeII	GATC	5 cut(s)	66, 838, 1134, 1348, 1551
NlaIII	CATG	9 cut(s)	59, 482, 742, 910, 965, 1244, 1254, 1408, 1573
PagI	TCATGA	1 cut(s)	1240
PceI	AGGCCT	1 cut(s)	687
PctI	GAATGC	2 cut(s)	250, 1067
PdmI	GAANNNNTTC	1 cut(s)	1225
PfeI	GAWTC	2 cut(s)	974, 1303
PflFI	GACNNNGTC	1 cut(s)	602
PleI	GAGTC	1 cut(s)	1179
PpsI	GAGTC	1 cut(s)	1179
PpuMI	RGGWCCY	3 cut(s)	598, 1541, 1574
Psp5II	RGGWCCY	3 cut(s)	598, 1541, 1574
Psp6I	CCWGG	5 cut(s)	615, 643, 1056, 1311, 1554
PspGI	CCWGG	5 cut(s)	615, 643, 1056, 1311, 1554
PspPI	GGNCC	5 cut(s)	598, 998, 1484, 1541, 1574
PspPPI	RGGWCCY	3 cut(s)	598, 1541, 1574
PstI	CTGCAG	2 cut(s)	154, 229
PstNI	CAGNNNCTG	1 cut(s)	977
PsuI	RGATCY	2 cut(s)	1134, 1551
PsyI	GACNNNGTC	1 cut(s)	602
RsaI	GTAC	6 cut(s)	359, 488, 508, 550, 734, 1467
RsaNI	GTAC	6 cut(s)	358, 487, 507, 549, 733, 1466
RseI	CAYNNNNRTG	2 cut(s)	867, 1517
SaqAI	TTAA	6 cut(s)	48, 335, 588, 744, 1296, 1335
Sau3AI	GATC	5 cut(s)	66, 838, 1134, 1348, 1551
Sau96I	GGNCC	5 cut(s)	598, 998, 1484, 1541, 1574
ScaI	AGTACT	1 cut(s)	359
SchI	GAGTC	1 cut(s)	1180
ScrFI	CCNGG	7 cut(s)	617, 645, 1058, 1313, 1537, 1538, 1556
SduI	GDGCHC	1 cut(s)	1160
SfaNI	GCATC	4 cut(s)	196, 340, 1234, 1272
SfcI	CTRYAG	2 cut(s)	150, 225
SinI	GGWCC	5 cut(s)	598, 998, 1484, 1541, 1574
SmaI	CCCGGG	1 cut(s)	1538
SmiMI	CAYNNNNRTG	2 cut(s)	867, 1517
SmlI	CTYRAG	2 cut(s)	1295, 1469
SmoI	CTYRAG	2 cut(s)	1295, 1469
SpeI	ACTAGT	2 cut(s)	635, 1514
Sse9I	AATT	4 cut(s)	75, 620, 966, 1146
SseBI	AGGCCT	1 cut(s)	687
SsiI	CCGC	4 cut(s)	217, 230, 790, 1445
SspMI	CTAG	2 cut(s)	636, 1515
StuI	AGGCCT	1 cut(s)	687
StyD4I	CCNGG	7 cut(s)	615, 643, 1056, 1311, 1535, 1536, 1554
StyI	CCWWGG	2 cut(s)	94, 688
TaaI	ACNGT	7 cut(s)	403, 452, 543, 1009, 1083, 1217, 1588
TaiI	ACGT	2 cut(s)	1396, 1566
TaqI	TCGA	7 cut(s)	122, 266, 411, 443, 503, 627, 903
TasI	AATT	4 cut(s)	75, 620, 966, 1146
TatI	WGTACW	4 cut(s)	357, 486, 506, 1465
TauI	GCSGC	1 cut(s)	220
TfiI	GAWTC	2 cut(s)	974, 1303
Tru1I	TTAA	6 cut(s)	48, 335, 588, 744, 1296, 1335
Tru9I	TTAA	6 cut(s)	48, 335, 588, 744, 1296, 1335
TscAI	CASTG	8 cut(s)	169, 406, 421, 732, 774, 1014, 1502, 1591
TseI	GCWGC	9 cut(s)	152, 224, 227, 420, 662, 797, 800, 1105, 1628
TspDTI	ATGAA	7 cut(s)	17, 23, 72, 800, 1229, 1239, 1558
TspGWI	ACGGA	1 cut(s)	429
TspMI	CCCGGG	1 cut(s)	1536
TspRI	CASTG	8 cut(s)	169, 406, 421, 732, 774, 1014, 1502, 1591
Tth111I	GACNNNGTC	1 cut(s)	602
Vha464I	CTTAAG	1 cut(s)	1295
VneI	GTGCAC	1 cut(s)	1156
VpaK11BI	GGWCC	5 cut(s)	598, 998, 1484, 1541, 1574
XapI	RAATTY	1 cut(s)	620
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1502
XmaI	CCCGGG	1 cut(s)	1536
XmnI	GAANNNNTTC	1 cut(s)	1225
XspI	CTAG	2 cut(s)	636, 1515
ZraI	GACGTC	1 cut(s)	1564
ZrmI	AGTACT	1 cut(s)	359

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.