FvH4_3g02660

dnaJ homolog subfamily C member 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013208
Physical Location & Seq
Reverse (-)
1347333 .. 1351040
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02660.t1

Sequence Viewer

Length: 906 bp
ATGGCGCCACCGGCTCCGATTCGGTGGTTCGCGGTCGCTGCTTCAGCTCTAATGCTCTGCCTCCTCATCTCTCCATCGGTGGCGATCTACTGCGACGAAGACGACTGCTACGATCTCCTCGGGGTTACGCAGAGCGCCAACTCGTCGGAAATCAAGAAAGCTTACTATAAGCTCTCTTTGAAACATCATCCGGATAAGAATCCGGATCCGGAATCGAAGAAGCTGTTTGTGAAGATTGCAAATGCTTATGAGATTCTGAAGGACGAGGCGTCGCGGGAGCAGTATGATTATGCAATTGCGCATCCTGAGGAGTATTTCTACAATACAGCTCGCTACTATCAAGCATATTATGGTCACAAAACGGACCCTCGTGCTGTACTGGTTGGCTTTCTTTTGGTTCTTTCAGCATTTCAGTATTTCAATCAGTGGACAAGGTATAATCAGGCTATAGATATGGTTAAAAAGACGCCTGCTTACAAAAACAGGCTCCGGCAATTGGAACTTGAGCGTAGGGGGGGGACTACAACTAAGAAGAAGAGTAACAAGCAGACAAACAAGAAAATGGATGAAGATCTCAGCAAAGAACTTGAACTGCAGATCAAAGGAGCTGAGCAACCCTCCATGTGGGATCTTATTTGTGTTCGTTTCGTACTGTTACCCTACACTATTGGCAAGCTGCTATTATGGCATGGCTGTTGGTTCTGGAGATACAAGGTGAAACAAGCTCCATATACATGGGACGATGCTTCCTATCTGACACAGAGATCCCTTAGTGCGCCGCTTGACGCATGGAGAAATGTTGATGAATCACGAAAGGAAGATCTTATCAATAGACGCTTATGGGAAGGTTCTAACTTGGAGAGCTATTATGCTGAGATGCGAAAAGAATCTAAGCGCAGAAGATAG

Protein Analysis

302

Amino Acids

35.44

Weight (kDa)

9.08

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 35 - 96 6.3e-24 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61770
fragaria_vesca FvH4_3g02660
malus_domestica MD05G1341100.v1.1 MD10G1314600.v1.1
prunus_persica Prupe.4G026200_v2.0.a1 Prupe.4G026200_v2.0.a1
pyrus_communis pycom05g31040 pycom10g26670
rosa_chinensis RchiOBHm_Chr5g0003981
rosa_laevigata RLG00000031194
rosa_multiflora Rmu_sc0000547.1_g000013
rosa_roxburghii Rroxscaffold_1G00071980
rosa_rugosa Rorug04G0408700
rosa_samantha Rh5AG036400 Rh5BG035500 Rh5CG039100 Rh5DG035000
rosa_wichuraiana Rw5G003310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 2 cut(s) 32, 274
AccIII TCCGGA 3 cut(s) 190, 202, 208
AciI CCGC 3 cut(s) 32, 274, 779
AclWI GGATC 4 cut(s) 200, 213, 636, 759
AcuI CTGAAG 2 cut(s) 27, 278
AcyI GRCGYC 3 cut(s) 5, 269, 467
AfaI GTAC 2 cut(s) 378, 651
AfiI CCNNNNNNNGG 2 cut(s) 496, 624
AgsI TTSAA 3 cut(s) 181, 421, 590
AhdI GACNNNNNGTC 1 cut(s) 268
AluBI AGCT 9 cut(s) 47, 161, 172, 223, 329, 608, 676, 725, 864
AluI AGCT 9 cut(s) 47, 161, 172, 223, 329, 608, 676, 725, 864
AlwI GGATC 4 cut(s) 200, 213, 636, 759
Ama87I CYCGRG 1 cut(s) 119
Aor13HI TCCGGA 3 cut(s) 190, 202, 208
ApeKI GCWGC 2 cut(s) 38, 676
AspLEI GCGC 5 cut(s) 7, 137, 301, 778, 897
AspS9I GGNCC 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 727
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 1 cut(s) 364
AxyI CCTNAGG 1 cut(s) 306
BamHI GGATCC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 25, 663
BccI CCATC 1 cut(s) 82
BfmI CTRYAG 2 cut(s) 447, 593
BfoI RGCGCY 2 cut(s) 8, 138
BglII AGATCT 2 cut(s) 571, 820
BisI GCNGC 3 cut(s) 39, 677, 779
BlpI GCTNAGC 1 cut(s) 609
BlsI GCNGC 3 cut(s) 40, 678, 780
Bme18I GGWCC 1 cut(s) 364
BmeRI GACNNNNNGTC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 364
BmiI GGNNCC 5 cut(s) 6, 15, 207, 366, 488
BmsI GCATC 3 cut(s) 310, 733, 867
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 602, 634
BpmI CTGGAG 1 cut(s) 724
Bpu1102I GCTNAGC 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 524
BsaBI GATNNNNATC 2 cut(s) 198, 570
BsaHI GRCGYC 3 cut(s) 5, 269, 467
BsaJI CCNNGG 1 cut(s) 118
BsaWI WCCGGW 3 cut(s) 190, 202, 208
Bsc4I CCNNNNNNNGG 2 cut(s) 496, 624
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 384
Bse21I CCTNAGG 1 cut(s) 306
Bse8I GATNNNNATC 2 cut(s) 198, 570
BseAI TCCGGA 3 cut(s) 190, 202, 208
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 3 cut(s) 187, 301, 571
BseJI GATNNNNATC 2 cut(s) 198, 570
BseLI CCNNNNNNNGG 2 cut(s) 496, 624
BseMII CTCAG 4 cut(s) 297, 589, 600, 864
BseNI ACTGG 1 cut(s) 384
BseRI GAGGAG 3 cut(s) 53, 107, 323
BseXI GCAGC 2 cut(s) 25, 663
Bsh1236I CGCG 2 cut(s) 32, 274
Bsh1285I CGRYCG 1 cut(s) 36
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 5 cut(s) 11, 191, 203, 209, 490
BslFI GGGAC 2 cut(s) 532, 752
BslI CCNNNNNNNGG 2 cut(s) 496, 624
BsmFI GGGAC 2 cut(s) 532, 752
BsoBI CYCGRG 1 cut(s) 119
Bsp13I TCCGGA 3 cut(s) 190, 202, 208
Bsp143I GATC 8 cut(s) 84, 112, 205, 571, 597, 628, 764, 820
Bsp1720I GCTNAGC 1 cut(s) 609
BspACI CCGC 3 cut(s) 32, 274, 779
BspCNI CTCAG 4 cut(s) 298, 588, 601, 865
BspEI TCCGGA 3 cut(s) 190, 202, 208
BspFNI CGCG 2 cut(s) 32, 274
BspLI GGNNCC 5 cut(s) 6, 15, 207, 366, 488
BspMAI CTGCAG 1 cut(s) 597
BspPI GGATC 4 cut(s) 200, 213, 636, 759
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 1 cut(s) 384
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 8 cut(s) 84, 112, 205, 571, 597, 628, 764, 820
BssNI GRCGYC 3 cut(s) 5, 269, 467
BssSI CACGAG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 654
Bst6I CTCTTC 1 cut(s) 530
BstACI GRCGYC 3 cut(s) 5, 269, 467
BstC8I GCNNGC 3 cut(s) 331, 471, 674
BstDEI CTNAG 7 cut(s) 306, 528, 575, 609, 770, 873, 891
BstF5I GGATG 3 cut(s) 187, 301, 571
BstFNI CGCG 2 cut(s) 32, 274
BstH2I RGCGCY 2 cut(s) 8, 138
BstHHI GCGC 5 cut(s) 7, 137, 301, 778, 897
BstKTI GATC 8 cut(s) 87, 115, 208, 574, 600, 631, 767, 823
BstMBI GATC 8 cut(s) 84, 112, 205, 571, 597, 628, 764, 820
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 4 cut(s) 11, 38, 44, 685
BstSFI CTRYAG 2 cut(s) 447, 593
BstUI CGCG 2 cut(s) 32, 274
BstV1I GCAGC 2 cut(s) 25, 663
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 5 cut(s) 205, 571, 628, 764, 820
BstXI CCANNNNNNTGG 1 cut(s) 735
BstYI RGATCY 5 cut(s) 205, 571, 628, 764, 820
Bsu36I CCTNAGG 1 cut(s) 306
BtsCI GGATG 3 cut(s) 187, 301, 571
BtsIMutI CAGTG 1 cut(s) 431
Cac8I GCNNGC 3 cut(s) 331, 471, 674
CfoI GCGC 5 cut(s) 7, 137, 301, 778, 897
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 364
CseI GACGC 4 cut(s) 258, 475, 794, 843
Csp6I GTAC 2 cut(s) 377, 650
CviAII CATG 4 cut(s) 622, 689, 735, 789
CviQI GTAC 2 cut(s) 377, 650
DdeI CTNAG 7 cut(s) 306, 528, 575, 609, 770, 873, 891
DinI GGCGCC 1 cut(s) 6
DpnI GATC 8 cut(s) 86, 114, 207, 573, 599, 630, 766, 822
DpnII GATC 8 cut(s) 84, 112, 205, 571, 597, 628, 764, 820
DriI GACNNNNNGTC 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 530
Eam1105I GACNNNNNGTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 530
Eco47I GGWCC 1 cut(s) 364
Eco57I CTGAAG 2 cut(s) 27, 278
Eco81I CCTNAGG 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 119
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
FaeI CATG 4 cut(s) 625, 692, 738, 792
FaqI GGGAC 2 cut(s) 532, 752
FatI CATG 4 cut(s) 621, 688, 734, 788
FauI CCCGC 1 cut(s) 267
Fnu4HI GCNGC 3 cut(s) 39, 677, 779
FokI GGATG 3 cut(s) 174, 288, 578
Fsp4HI GCNGC 3 cut(s) 39, 677, 779
FspI TGCGCA 1 cut(s) 300
GlaI GCGC 5 cut(s) 6, 136, 300, 777, 896
GluI GCNGC 3 cut(s) 39, 677, 779
GsuI CTGGAG 1 cut(s) 724
HaeII RGCGCY 2 cut(s) 8, 138
HapII CCGG 5 cut(s) 11, 191, 203, 209, 490
HgaI GACGC 4 cut(s) 258, 475, 794, 843
HhaI GCGC 5 cut(s) 7, 137, 301, 778, 897
Hin1I GRCGYC 3 cut(s) 5, 269, 467
Hin1II CATG 4 cut(s) 625, 692, 738, 792
Hin6I GCGC 5 cut(s) 5, 135, 299, 776, 895
HinP1I GCGC 5 cut(s) 5, 135, 299, 776, 895
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 6 cut(s) 19, 199, 212, 253, 806, 887
HpaII CCGG 5 cut(s) 11, 191, 203, 209, 490
HphI GGTGA 1 cut(s) 727
Hpy166II GTNNAC 1 cut(s) 429
Hpy188I TCNGA 4 cut(s) 18, 148, 258, 756
Hpy188III TCNNGA 7 cut(s) 154, 191, 203, 209, 305, 703, 810
Hpy8I GTNNAC 1 cut(s) 429
Hpy99I CGWCG 3 cut(s) 98, 148, 274
HpyAV CCTTC 2 cut(s) 253, 839
HpyCH4III ACNGT 1 cut(s) 654
HpyCH4V TGCA 3 cut(s) 239, 293, 595
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 38, 44, 685
HpyF3I CTNAG 7 cut(s) 306, 528, 575, 609, 770, 873, 891
Hsp92I GRCGYC 3 cut(s) 5, 269, 467
Hsp92II CATG 4 cut(s) 625, 692, 738, 792
HspAI GCGC 5 cut(s) 5, 135, 299, 776, 895
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 3 cut(s) 190, 202, 208
Kzo9I GATC 8 cut(s) 84, 112, 205, 571, 597, 628, 764, 820
LmnI GCTCC 5 cut(s) 19, 277, 492, 605, 730
Lsp1109I GCAGC 2 cut(s) 25, 663
LweI GCATC 3 cut(s) 310, 733, 867
MaeIII GTNAC 4 cut(s) 124, 353, 539, 654
MalI GATC 8 cut(s) 86, 114, 207, 573, 599, 630, 766, 822
MboI GATC 8 cut(s) 84, 112, 205, 571, 597, 628, 764, 820
MboII GAAGA 7 cut(s) 110, 229, 244, 544, 547, 581, 830
MfeI CAATTG 2 cut(s) 294, 494
MflI RGATCY 5 cut(s) 205, 571, 628, 764, 820
MluCI AATT 2 cut(s) 294, 494
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 7 cut(s) 71, 74, 128, 259, 301, 378, 628
MroI TCCGGA 3 cut(s) 190, 202, 208
MseI TTAA 1 cut(s) 459
MslI CAYNNNNRTG 1 cut(s) 733
MspI CCGG 5 cut(s) 11, 191, 203, 209, 490
MunI CAATTG 2 cut(s) 294, 494
MvnI CGCG 2 cut(s) 32, 274
MwoI GCNNNNNNNGC 4 cut(s) 11, 38, 44, 685
NarI GGCGCC 1 cut(s) 5
NdeII GATC 8 cut(s) 84, 112, 205, 571, 597, 628, 764, 820
NlaIII CATG 4 cut(s) 625, 692, 738, 792
NlaIV GGNNCC 5 cut(s) 6, 15, 207, 366, 488
NmuCI GTSAC 1 cut(s) 353
NsbI TGCGCA 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 6 cut(s) 19, 199, 212, 253, 806, 887
PkrI GCNGC 3 cut(s) 40, 678, 780
PluTI GGCGCC 1 cut(s) 8
PspN4I GGNNCC 5 cut(s) 6, 15, 207, 366, 488
PspPI GGNCC 1 cut(s) 364
PstI CTGCAG 1 cut(s) 597
PsuI RGATCY 5 cut(s) 205, 571, 628, 764, 820
RsaI GTAC 2 cut(s) 378, 651
RsaNI GTAC 2 cut(s) 377, 650
RseI CAYNNNNRTG 1 cut(s) 733
SaqAI TTAA 1 cut(s) 459
SatI GCNGC 3 cut(s) 39, 677, 779
Sau3AI GATC 8 cut(s) 84, 112, 205, 571, 597, 628, 764, 820
Sau96I GGNCC 1 cut(s) 364
SfaNI GCATC 3 cut(s) 310, 733, 867
SfcI CTRYAG 2 cut(s) 447, 593
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 733
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 2 cut(s) 294, 494
SsiI CCGC 3 cut(s) 32, 274, 779
SspDI GGCGCC 1 cut(s) 4
TaaI ACNGT 1 cut(s) 654
TaqI TCGA 1 cut(s) 215
TasI AATT 2 cut(s) 294, 494
TatI WGTACW 1 cut(s) 376
TauI GCSGC 1 cut(s) 781
TfiI GAWTC 6 cut(s) 19, 199, 212, 253, 806, 887
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 1 cut(s) 431
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 2 cut(s) 38, 676
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 2 cut(s) 582, 819
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.