Rh5CG039100

dnaJ homolog subfamily C member 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013208
Physical Location & Seq
Reverse (-)
2476268 .. 2479945
3678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG039100.1

Sequence Viewer

Length: 996 bp
ATGTTTCACTACAAAGGCAGGGAGAGGGAGAGAGAGAGAGAGTGTTTGCAGGGAAACCATTTCAGAGGAAATCTAGAGAGCTCCATCGTCGCCATGGCGCCACCGGCATCGATTCGGTGGTTCGCGGCCGCTTCAGCTCTAATGCTCTGCCTTCTCATCTCTCCATCCACCGCCATCTACTGCGACGAAGACGACTGCTACGATCTCCTCGGGGTTACGCAGAGCGCAAACTCGTCGGAAATCAAGAAAGCGTACTATAAGCTATCTTTGAAGCATCACCCGGATAAGAACCCGGATCCGGAATCGAAAAAGCTGTTTGTTAAAATTGCAAATGCTTATGAGATTCTGAAGGACGAGGCGTCGCGGGAGCAGTATGATTATGCAATTGCACATCCTGAGGAGTATTTTTACAATACAGCTCGCTACTATCAAGCATATTATGGTCACAAGACGGATCCTCGTGCTGTACTGGTTGGCTTTCTTTTGGTTCTTTCAGCATTTCAGTATTTCAATCAATGGACAAGGTATAATCAGGCTATAGACATGGTTAAAAAGACGCCTGCTTACAAAAATAGACTTCGGCAATTGGAACTTGAGCGTAGTGGGGGGACTACAACTAAGAAGAAGAGTAACAAGCAGACAAACAAGAAAATGGATGAAGATCTCAGCAAAGAACTTGAATTGCAGATTACAGGTGCTGAGCAACCCTCCATGTGGGATCTTCTTGGTGTCCGTTTCATACTGTTACCCTACACTATTGGCAAGCTGCTGTTATGGCATGGCTGTTGGTTCTGGAGATACAAGGTGAAACAAGCTCCATATACATGGGATGATGCCTCCTACCTGACACAGAGATCCCTTAGTGCGCCACTCGACGCATGGAGAAATGTTGATGACTTACGAAAGGAAGATCTTATCAATAGACGTTTATGGGAAGGTTCGAACTTGGAGAGCTATTATGCGGAGATGCGAAAAGAATCCAAGCGCAGAAGATAG

Protein Analysis

331

Amino Acids

38.95

Weight (kDa)

9.0

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 65 - 126 7.4e-24 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61770
fragaria_vesca FvH4_3g02660
malus_domestica MD05G1341100.v1.1 MD10G1314600.v1.1
prunus_persica Prupe.4G026200_v2.0.a1 Prupe.4G026200_v2.0.a1
pyrus_communis pycom05g31040 pycom10g26670
rosa_chinensis RchiOBHm_Chr5g0003981
rosa_laevigata RLG00000031194
rosa_multiflora Rmu_sc0000547.1_g000013
rosa_roxburghii Rroxscaffold_1G00071980
rosa_rugosa Rorug04G0408700
rosa_samantha Rh5AG036400 Rh5BG035500 Rh5CG039100 Rh5DG035000
rosa_wichuraiana Rw5G003310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 97
AccII CGCG 2 cut(s) 125, 364
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 5 cut(s) 125, 129, 171, 364, 962
AclWI GGATC 6 cut(s) 290, 303, 449, 462, 726, 849
AcoI YGGCCR 1 cut(s) 126
AcuI CTGAAG 2 cut(s) 117, 368
AcyI GRCGYC 3 cut(s) 98, 359, 557
AfaI GTAC 2 cut(s) 254, 468
AfiI CCNNNNNNNGG 2 cut(s) 298, 714
AgsI TTSAA 3 cut(s) 271, 511, 680
AhdI GACNNNNNGTC 1 cut(s) 358
AluBI AGCT 8 cut(s) 81, 137, 262, 313, 419, 766, 815, 954
AluI AGCT 8 cut(s) 81, 137, 262, 313, 419, 766, 815, 954
Alw21I GWGCWC 1 cut(s) 83
AlwI GGATC 6 cut(s) 290, 303, 449, 462, 726, 849
AlwNI CAGNNNCTG 1 cut(s) 698
Ama87I CYCGRG 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 766
AspLEI GCGC 4 cut(s) 100, 227, 868, 987
AsuC2I CCSGG 2 cut(s) 281, 293
AsuHPI GGTGA 2 cut(s) 269, 817
AsuII TTCGAA 1 cut(s) 941
AvaI CYCGRG 1 cut(s) 209
AxyI CCTNAGG 1 cut(s) 396
BamHI GGATCC 2 cut(s) 295, 454
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 83
BauI CACGAG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 1 cut(s) 753
BccI CCATC 3 cut(s) 92, 172, 182
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BcnI CCSGG 2 cut(s) 281, 293
BfaI CTAG 1 cut(s) 74
BfmI CTRYAG 1 cut(s) 537
BfoI RGCGCY 1 cut(s) 101
BglII AGATCT 2 cut(s) 661, 910
BisI GCNGC 3 cut(s) 126, 129, 767
BlpI GCTNAGC 1 cut(s) 699
BlsI GCNGC 3 cut(s) 127, 130, 768
Bme1390I CCNGG 2 cut(s) 281, 293
BmeRI GACNNNNNGTC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 209
BmiI GGNNCC 3 cut(s) 99, 297, 456
BmrFI CCNGG 2 cut(s) 281, 293
BmsI GCATC 4 cut(s) 116, 283, 823, 957
BpiI GAAGAC 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 692, 724
BpmI CTGGAG 1 cut(s) 814
Bpu1102I GCTNAGC 1 cut(s) 699
Bpu14I TTCGAA 1 cut(s) 941
BpuEI CTTGAG 1 cut(s) 614
BpuMI CCSGG 2 cut(s) 281, 293
Bsa29I ATCGAT 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 3 cut(s) 98, 359, 557
BsaJI CCNNGG 2 cut(s) 93, 208
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 714
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 474
Bse21I CCTNAGG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 660
BseAI TCCGGA 1 cut(s) 298
BseCI ATCGAT 1 cut(s) 110
BseDI CCNNGG 2 cut(s) 93, 208
BseGI GGATG 4 cut(s) 164, 391, 661, 835
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 2 cut(s) 298, 714
BseMII CTCAG 3 cut(s) 387, 679, 690
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 2 cut(s) 197, 413
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 1 cut(s) 753
Bsh1236I CGCG 2 cut(s) 125, 364
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 1 cut(s) 128
BshNI GGYRCC 1 cut(s) 97
BshVI ATCGAT 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 4 cut(s) 104, 281, 293, 299
BslFI GGGAC 1 cut(s) 622
BslI CCNNNNNNNGG 2 cut(s) 298, 714
BsmFI GGGAC 1 cut(s) 622
BsnI GGCC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 209
Bsp119I TTCGAA 1 cut(s) 941
Bsp1286I GDGCHC 1 cut(s) 83
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 7 cut(s) 202, 295, 454, 661, 718, 854, 910
Bsp1720I GCTNAGC 1 cut(s) 699
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 5 cut(s) 125, 129, 171, 364, 962
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 3 cut(s) 388, 678, 691
BspDI ATCGAT 1 cut(s) 110
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 2 cut(s) 125, 364
BspLI GGNNCC 3 cut(s) 99, 297, 456
BspPI GGATC 6 cut(s) 290, 303, 449, 462, 726, 849
BspT104I TTCGAA 1 cut(s) 941
BspT107I GGYRCC 1 cut(s) 97
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 1 cut(s) 474
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 2 cut(s) 93, 208
BssMI GATC 7 cut(s) 202, 295, 454, 661, 718, 854, 910
BssNI GRCGYC 3 cut(s) 98, 359, 557
BssSI CACGAG 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 93
Bst2BI CACGAG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 744
Bst6I CTCTTC 1 cut(s) 620
BstACI GRCGYC 3 cut(s) 98, 359, 557
BstBI TTCGAA 1 cut(s) 941
BstC8I GCNNGC 3 cut(s) 421, 561, 764
BstDEI CTNAG 5 cut(s) 396, 618, 665, 699, 860
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 4 cut(s) 164, 391, 661, 835
BstFNI CGCG 2 cut(s) 125, 364
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 4 cut(s) 100, 227, 868, 987
BstKTI GATC 7 cut(s) 205, 298, 457, 664, 721, 857, 913
BstMBI GATC 7 cut(s) 202, 295, 454, 661, 718, 854, 910
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 3 cut(s) 104, 134, 775
BstSCI CCNGG 2 cut(s) 279, 291
BstSFI CTRYAG 1 cut(s) 537
BstUI CGCG 2 cut(s) 125, 364
BstV1I GCAGC 1 cut(s) 753
BstV2I GAAGAC 1 cut(s) 195
BstX2I RGATCY 6 cut(s) 295, 454, 661, 718, 854, 910
BstXI CCANNNNNNTGG 1 cut(s) 825
BstYI RGATCY 6 cut(s) 295, 454, 661, 718, 854, 910
BstZI CGGCCG 1 cut(s) 126
Bsu15I ATCGAT 1 cut(s) 110
Bsu36I CCTNAGG 1 cut(s) 396
BsuRI GGCC 1 cut(s) 128
BsuTUI ATCGAT 1 cut(s) 110
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 4 cut(s) 164, 391, 661, 835
Cac8I GCNNGC 3 cut(s) 421, 561, 764
CaiI CAGNNNCTG 1 cut(s) 698
CciNI GCGGCCGC 1 cut(s) 126
CfoI GCGC 4 cut(s) 100, 227, 868, 987
Cfr10I RCCGGY 1 cut(s) 103
ClaI ATCGAT 1 cut(s) 110
CseI GACGC 3 cut(s) 348, 565, 884
Csp6I GTAC 2 cut(s) 253, 467
CviAII CATG 6 cut(s) 94, 544, 712, 779, 825, 879
CviQI GTAC 2 cut(s) 253, 467
DdeI CTNAG 5 cut(s) 396, 618, 665, 699, 860
DinI GGCGCC 1 cut(s) 99
DpnI GATC 7 cut(s) 204, 297, 456, 663, 720, 856, 912
DpnII GATC 7 cut(s) 202, 295, 454, 661, 718, 854, 910
DriI GACNNNNNGTC 1 cut(s) 358
EaeI YGGCCR 1 cut(s) 126
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 620
Eam1105I GACNNNNNGTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 620
Ecl136II GAGCTC 1 cut(s) 81
EclXI CGGCCG 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 83
Eco52I CGGCCG 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 81
Eco57I CTGAAG 2 cut(s) 117, 368
Eco81I CCTNAGG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 83
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 6 cut(s) 97, 547, 715, 782, 828, 882
FaqI GGGAC 1 cut(s) 622
FatI CATG 6 cut(s) 93, 543, 711, 778, 824, 878
FauI CCCGC 1 cut(s) 357
Fnu4HI GCNGC 3 cut(s) 126, 129, 767
FokI GGATG 4 cut(s) 151, 378, 668, 842
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 3 cut(s) 126, 129, 767
FspBI CTAG 1 cut(s) 74
GlaI GCGC 4 cut(s) 99, 226, 867, 986
GluI GCNGC 3 cut(s) 126, 129, 767
GsuI CTGGAG 1 cut(s) 814
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 1 cut(s) 128
HapII CCGG 4 cut(s) 104, 281, 293, 299
HgaI GACGC 3 cut(s) 348, 565, 884
HhaI GCGC 4 cut(s) 100, 227, 868, 987
Hin1I GRCGYC 3 cut(s) 98, 359, 557
Hin1II CATG 6 cut(s) 97, 547, 715, 782, 828, 882
Hin6I GCGC 4 cut(s) 98, 225, 866, 985
HinP1I GCGC 4 cut(s) 98, 225, 866, 985
HinfI GANTC 4 cut(s) 112, 302, 343, 977
HpaII CCGG 4 cut(s) 104, 281, 293, 299
HphI GGTGA 2 cut(s) 269, 817
Hpy188I TCNGA 3 cut(s) 65, 238, 348
Hpy188III TCNNGA 5 cut(s) 74, 244, 299, 395, 793
Hpy99I CGWCG 5 cut(s) 92, 188, 238, 364, 878
HpyAV CCTTC 3 cut(s) 161, 343, 929
HpyCH4III ACNGT 1 cut(s) 744
HpyCH4IV ACGT 1 cut(s) 925
HpyCH4V TGCA 5 cut(s) 49, 329, 383, 389, 685
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 134, 775
HpyF3I CTNAG 5 cut(s) 396, 618, 665, 699, 860
HpySE526I ACGT 1 cut(s) 925
Hsp92I GRCGYC 3 cut(s) 98, 359, 557
Hsp92II CATG 6 cut(s) 97, 547, 715, 782, 828, 882
HspAI GCGC 4 cut(s) 98, 225, 866, 985
KasI GGCGCC 1 cut(s) 97
Kpn2I TCCGGA 1 cut(s) 298
Kzo9I GATC 7 cut(s) 202, 295, 454, 661, 718, 854, 910
LmnI GCTCC 3 cut(s) 86, 367, 820
Lsp1109I GCAGC 1 cut(s) 753
LweI GCATC 4 cut(s) 116, 283, 823, 957
MaeI CTAG 1 cut(s) 74
MaeII ACGT 1 cut(s) 925
MaeIII GTNAC 4 cut(s) 214, 443, 629, 744
MalI GATC 7 cut(s) 204, 297, 456, 663, 720, 856, 912
MboI GATC 7 cut(s) 202, 295, 454, 661, 718, 854, 910
MboII GAAGA 6 cut(s) 200, 634, 637, 671, 713, 920
MfeI CAATTG 2 cut(s) 384, 584
MflI RGATCY 6 cut(s) 295, 454, 661, 718, 854, 910
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 4 cut(s) 324, 384, 584, 680
Mly113I GGCGCC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 8 cut(s) 18, 59, 218, 349, 391, 468, 718, 847
MroI TCCGGA 1 cut(s) 298
MseI TTAA 2 cut(s) 321, 549
MslI CAYNNNNRTG 1 cut(s) 823
MspI CCGG 4 cut(s) 104, 281, 293, 299
MspR9I CCNGG 2 cut(s) 281, 293
MunI CAATTG 2 cut(s) 384, 584
MvnI CGCG 2 cut(s) 125, 364
MwoI GCNNNNNNNGC 3 cut(s) 104, 134, 775
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 2 cut(s) 281, 293
NcoI CCATGG 1 cut(s) 93
NdeII GATC 7 cut(s) 202, 295, 454, 661, 718, 854, 910
NlaIII CATG 6 cut(s) 97, 547, 715, 782, 828, 882
NlaIV GGNNCC 3 cut(s) 99, 297, 456
NmuCI GTSAC 1 cut(s) 443
NotI GCGGCCGC 1 cut(s) 126
NspV TTCGAA 1 cut(s) 941
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 4 cut(s) 112, 302, 343, 977
PkrI GCNGC 3 cut(s) 127, 130, 768
PluTI GGCGCC 1 cut(s) 101
Psp124BI GAGCTC 1 cut(s) 83
PspN4I GGNNCC 3 cut(s) 99, 297, 456
PstNI CAGNNNCTG 1 cut(s) 698
PsuI RGATCY 6 cut(s) 295, 454, 661, 718, 854, 910
RsaI GTAC 2 cut(s) 254, 468
RsaNI GTAC 2 cut(s) 253, 467
RseI CAYNNNNRTG 1 cut(s) 823
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 321, 549
SatI GCNGC 3 cut(s) 126, 129, 767
Sau3AI GATC 7 cut(s) 202, 295, 454, 661, 718, 854, 910
ScrFI CCNGG 2 cut(s) 281, 293
SduI GDGCHC 1 cut(s) 83
SfaNI GCATC 4 cut(s) 116, 283, 823, 957
SfcI CTRYAG 1 cut(s) 537
SfoI GGCGCC 1 cut(s) 99
SfuI TTCGAA 1 cut(s) 941
SmiMI CAYNNNNRTG 1 cut(s) 823
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
Sse9I AATT 4 cut(s) 324, 384, 584, 680
SsiI CCGC 5 cut(s) 125, 129, 171, 364, 962
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 1 cut(s) 74
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 279, 291
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 744
TaiI ACGT 1 cut(s) 928
TaqI TCGA 4 cut(s) 110, 305, 873, 941
TasI AATT 4 cut(s) 324, 384, 584, 680
TatI WGTACW 1 cut(s) 466
TauI GCSGC 2 cut(s) 128, 131
TfiI GAWTC 4 cut(s) 112, 302, 343, 977
Tru1I TTAA 2 cut(s) 321, 549
Tru9I TTAA 2 cut(s) 321, 549
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 1 cut(s) 766
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 2 cut(s) 672, 727
TspGWI ACGGA 2 cut(s) 467, 722
XbaI TCTAGA 1 cut(s) 73
XcmI CCANNNNNNNNNTGG 2 cut(s) 91, 876
XspI CTAG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.