pycom10g26670

dnaJ homolog subfamily C member 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013208
Physical Location & Seq
Forward (+)
27943353 .. 27945981
2629 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g26670.5

Sequence Viewer

Length: 924 bp
ATGGGTTTGGTCAGAGGATCCTCAGCGGCAAATCCAACGCGCTCCAACCCTCGGGAATCCATTATCAGAGAAATCCAGAGCTCCGTCGGAACCATGGCGCCACCGGCCGCGATTCGGTGGTTCTCGGTCGTTTTAGTTCTAATGCTGTGCTATCTCATCACTCCGTCTACGGCCATATACTGCGACGAGGACGACTGCTACGATCTCCTCGGGGTTACGCAGAGCGCCAATTCGTCGGAAATCAAGAAAGCTTACTACAAACTCTCATTGAAACATCACCCGGATAAAAACCCGGATCCGGAATCGAAAAAGCTGTTTGTGAAAATTGCCAATGCCTATGAGATTCTGAAAGACGAGGCGTCGCGAGAGCAGTATGATTATGCCATTGCACATCCAGAGGAGGTATTTTACAATACTGCTCGCTACTATCAAGCATATTATGGTCACAAAACGGATCCTCGTGCAGTCCTGGTTGGCCTTCTTTTGATTCTTTCTGCATTTCAGTATCTGAATCAGTGGACAAGGTATAATCAGGCTGTAGATATGGTTAAGAAGACTCCTGCTTACAAAAATAGACTACGGGCTTTGGAACTTGAGCGTAGTGGAGGGACTACAAATAAGAAAAAGAGCAACAGGCAGATGAACAAGAAAGTGGAAGAAGATCTTGGCAGCGAACTGGAGCTGCAGATTAACGGAGCGGAACAACCCTCCATGTGGGATCTTCTTGGTGTTCGTTTCATACTTCTTCCCTACACGATTGGCAAGCTATTATTATGGTGTGGCTGTTGGTTCTGGAGATACAAGGTGAAAAAAGCACCATATACATGGGATGATGCCTCCTACCTGACACGGAGGTCCCTTGGTGCGCCTATTGATGGATGGAGAAATCTAGATGAATCAAGAAAGGAAGATCTTATTTTGTGA

Protein Analysis

308

Amino Acids

35.32

Weight (kDa)

8.86

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61770
fragaria_vesca FvH4_3g02660
malus_domestica MD05G1341100.v1.1 MD10G1314600.v1.1
prunus_persica Prupe.4G026200_v2.0.a1 Prupe.4G026200_v2.0.a1
pyrus_communis pycom05g31040 pycom10g26670
rosa_chinensis RchiOBHm_Chr5g0003981
rosa_laevigata RLG00000031194
rosa_multiflora Rmu_sc0000547.1_g000013
rosa_roxburghii Rroxscaffold_1G00071980
rosa_rugosa Rorug04G0408700
rosa_samantha Rh5AG036400 Rh5BG035500 Rh5CG039100 Rh5DG035000
rosa_wichuraiana Rw5G003310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 853
AccB1I GGYRCC 1 cut(s) 97
AccBSI CCGCTC 1 cut(s) 698
AccI GTMKAC 1 cut(s) 167
AccII CGCG 3 cut(s) 40, 110, 364
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 3 cut(s) 26, 108, 698
AclWI GGATC 7 cut(s) 12, 25, 290, 303, 449, 462, 726
AcoI YGGCCR 2 cut(s) 105, 171
AcyI GRCGYC 2 cut(s) 98, 359
AfiI CCNNNNNNNGG 5 cut(s) 51, 114, 298, 714, 875
AgsI TTSAA 1 cut(s) 271
AhdI GACNNNNNGTC 1 cut(s) 358
AjnI CCWGG 1 cut(s) 468
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AluBI AGCT 5 cut(s) 81, 251, 313, 682, 766
AluI AGCT 5 cut(s) 81, 251, 313, 682, 766
Alw21I GWGCWC 1 cut(s) 83
AlwI GGATC 7 cut(s) 12, 25, 290, 303, 449, 462, 726
AlwNI CAGNNNCTG 1 cut(s) 508
Ama87I CYCGRG 2 cut(s) 51, 209
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 3 cut(s) 105, 171, 475
ApeKI GCWGC 2 cut(s) 669, 682
AspLEI GCGC 4 cut(s) 42, 100, 227, 868
AspS9I GGNCC 1 cut(s) 855
AsuC2I CCSGG 2 cut(s) 281, 293
AsuHPI GGTGA 2 cut(s) 269, 817
AvaI CYCGRG 2 cut(s) 51, 209
AvaII GGWCC 1 cut(s) 855
BamHI GGATCC 3 cut(s) 17, 295, 454
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 83
BauI CACGAG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 560
Bbv12I GWGCWC 1 cut(s) 83
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 2 cut(s) 669, 681
BccI CCATC 2 cut(s) 869, 873
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 470
BcnI CCSGG 2 cut(s) 281, 293
BfaI CTAG 1 cut(s) 890
BfmI CTRYAG 2 cut(s) 537, 683
BfoI RGCGCY 2 cut(s) 101, 228
BglII AGATCT 2 cut(s) 661, 910
BisI GCNGC 4 cut(s) 27, 108, 670, 683
BlsI GCNGC 4 cut(s) 28, 109, 671, 684
Bme1390I CCNGG 3 cut(s) 281, 293, 470
Bme18I GGWCC 1 cut(s) 855
BmeRI GACNNNNNGTC 1 cut(s) 358
BmeT110I CYCGRG 2 cut(s) 51, 209
BmgT120I GGNCC 1 cut(s) 855
BmiI GGNNCC 6 cut(s) 19, 91, 99, 297, 456, 857
BmrFI CCNGG 3 cut(s) 281, 293, 470
BmsI GCATC 1 cut(s) 823
BpiI GAAGAC 1 cut(s) 560
BpmI CTGGAG 2 cut(s) 698, 814
Bpu10I CCTNAGC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 614
BpuMI CCSGG 2 cut(s) 281, 293
BsaHI GRCGYC 2 cut(s) 98, 359
BsaJI CCNNGG 4 cut(s) 50, 93, 208, 859
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 114, 298, 714, 875
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 681
Bse3DI GCAATG 1 cut(s) 384
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 1 cut(s) 470
BseDI CCNNGG 4 cut(s) 50, 93, 208, 859
BseGI GGATG 3 cut(s) 391, 835, 884
BseLI CCNNNNNNNGG 5 cut(s) 51, 114, 298, 714, 875
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 1 cut(s) 681
BseRI GAGGAG 2 cut(s) 197, 413
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 2 cut(s) 669, 681
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 3 cut(s) 40, 110, 364
Bsh1285I CGRYCG 2 cut(s) 108, 129
BshFI GGCC 3 cut(s) 107, 173, 477
BshNI GGYRCC 1 cut(s) 97
BsiEI CGRYCG 2 cut(s) 108, 129
BsiHKAI GWGCWC 1 cut(s) 83
BsiHKCI CYCGRG 2 cut(s) 51, 209
BsiSI CCGG 4 cut(s) 104, 281, 293, 299
BslFI GGGAC 2 cut(s) 622, 841
BslI CCNNNNNNNGG 5 cut(s) 51, 114, 298, 714, 875
BsmFI GGGAC 2 cut(s) 622, 841
BsnI GGCC 3 cut(s) 107, 173, 477
BsoBI CYCGRG 2 cut(s) 51, 209
Bsp1286I GDGCHC 1 cut(s) 83
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 7 cut(s) 17, 202, 295, 454, 661, 718, 910
Bsp19I CCATGG 1 cut(s) 93
Bsp68I TCGCGA 1 cut(s) 364
BspACI CCGC 3 cut(s) 26, 108, 698
BspANI GGCC 3 cut(s) 107, 173, 477
BspCNI CTCAG 1 cut(s) 35
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 3 cut(s) 40, 110, 364
BspLI GGNNCC 6 cut(s) 19, 91, 99, 297, 456, 857
BspMAI CTGCAG 1 cut(s) 687
BspPI GGATC 7 cut(s) 12, 25, 290, 303, 449, 462, 726
BspT107I GGYRCC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 384
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 1 cut(s) 681
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 4 cut(s) 50, 93, 208, 859
BssMI GATC 7 cut(s) 17, 202, 295, 454, 661, 718, 910
BssNI GRCGYC 2 cut(s) 98, 359
BssSI CACGAG 1 cut(s) 459
BssT1I CCWWGG 2 cut(s) 93, 859
Bst2BI CACGAG 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 470
BstACI GRCGYC 2 cut(s) 98, 359
BstC8I GCNNGC 2 cut(s) 421, 764
BstDEI CTNAG 1 cut(s) 22
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 3 cut(s) 391, 835, 884
BstFNI CGCG 3 cut(s) 40, 110, 364
BstH2I RGCGCY 2 cut(s) 101, 228
BstHHI GCGC 4 cut(s) 42, 100, 227, 868
BstKTI GATC 7 cut(s) 20, 205, 298, 457, 664, 721, 913
BstMBI GATC 7 cut(s) 17, 202, 295, 454, 661, 718, 910
BstMCI CGRYCG 2 cut(s) 108, 129
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstNI CCWGG 1 cut(s) 470
BstSCI CCNGG 3 cut(s) 279, 291, 468
BstSFI CTRYAG 2 cut(s) 537, 683
BstUI CGCG 3 cut(s) 40, 110, 364
BstV1I GCAGC 2 cut(s) 669, 681
BstV2I GAAGAC 1 cut(s) 560
BstX2I RGATCY 6 cut(s) 17, 295, 454, 661, 718, 910
BstXI CCANNNNNNTGG 1 cut(s) 825
BstYI RGATCY 6 cut(s) 17, 295, 454, 661, 718, 910
BstZI CGGCCG 1 cut(s) 105
BsuRI GGCC 3 cut(s) 107, 173, 477
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 3 cut(s) 391, 835, 884
BtsIMutI CAGTG 1 cut(s) 521
BtuMI TCGCGA 1 cut(s) 364
Cac8I GCNNGC 2 cut(s) 421, 764
CaiI CAGNNNCTG 1 cut(s) 508
CfoI GCGC 4 cut(s) 42, 100, 227, 868
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 1 cut(s) 855
CseI GACGC 1 cut(s) 348
CviAII CATG 3 cut(s) 94, 712, 825
DdeI CTNAG 1 cut(s) 22
DinI GGCGCC 1 cut(s) 99
DpnI GATC 7 cut(s) 19, 204, 297, 456, 663, 720, 912
DpnII GATC 7 cut(s) 17, 202, 295, 454, 661, 718, 910
DrdI GACNNNNNNGTC 1 cut(s) 853
DriI GACNNNNNGTC 1 cut(s) 358
DseDI GACNNNNNNGTC 1 cut(s) 853
EaeI YGGCCR 2 cut(s) 105, 171
EagI CGGCCG 1 cut(s) 105
Eam1105I GACNNNNNGTC 1 cut(s) 358
Ecl136II GAGCTC 1 cut(s) 81
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 93, 859
Eco24I GRGCYC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 855
Eco52I CGGCCG 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 81
Eco88I CYCGRG 2 cut(s) 51, 209
EcoICRI GAGCTC 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 855
EcoRII CCWGG 1 cut(s) 468
EcoT14I CCWWGG 2 cut(s) 93, 859
EcoT38I GRGCYC 1 cut(s) 83
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
ErhI CCWWGG 2 cut(s) 93, 859
FaeI CATG 3 cut(s) 97, 715, 828
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FaqI GGGAC 2 cut(s) 622, 841
FatI CATG 3 cut(s) 93, 711, 824
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 4 cut(s) 27, 108, 670, 683
FokI GGATG 3 cut(s) 378, 842, 891
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 4 cut(s) 27, 108, 670, 683
FspBI CTAG 1 cut(s) 890
GlaI GCGC 4 cut(s) 41, 99, 226, 867
GluI GCNGC 4 cut(s) 27, 108, 670, 683
GsuI CTGGAG 2 cut(s) 698, 814
HaeII RGCGCY 2 cut(s) 101, 228
HaeIII GGCC 3 cut(s) 107, 173, 477
HapII CCGG 4 cut(s) 104, 281, 293, 299
HgaI GACGC 1 cut(s) 348
HhaI GCGC 4 cut(s) 42, 100, 227, 868
Hin1I GRCGYC 2 cut(s) 98, 359
Hin1II CATG 3 cut(s) 97, 715, 828
Hin6I GCGC 4 cut(s) 40, 98, 225, 866
HinP1I GCGC 4 cut(s) 40, 98, 225, 866
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 8 cut(s) 56, 112, 302, 343, 487, 511, 556, 896
HpaII CCGG 4 cut(s) 104, 281, 293, 299
HphI GGTGA 2 cut(s) 269, 817
Hpy166II GTNNAC 2 cut(s) 168, 519
Hpy188I TCNGA 6 cut(s) 14, 68, 89, 238, 348, 510
Hpy188III TCNNGA 9 cut(s) 53, 76, 244, 299, 363, 395, 793, 890, 900
Hpy8I GTNNAC 2 cut(s) 168, 519
Hpy99I CGWCG 4 cut(s) 89, 188, 238, 364
HpyAV CCTTC 1 cut(s) 488
HpyCH4V TGCA 4 cut(s) 389, 464, 497, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 1 cut(s) 22
Hsp92I GRCGYC 2 cut(s) 98, 359
Hsp92II CATG 3 cut(s) 97, 715, 828
HspAI GCGC 4 cut(s) 40, 98, 225, 866
KasI GGCGCC 1 cut(s) 97
Kpn2I TCCGGA 1 cut(s) 298
Kzo9I GATC 7 cut(s) 17, 202, 295, 454, 661, 718, 910
LmnI GCTCC 4 cut(s) 47, 86, 679, 695
Lsp1109I GCAGC 2 cut(s) 669, 681
LweI GCATC 1 cut(s) 823
MaeI CTAG 1 cut(s) 890
MaeIII GTNAC 2 cut(s) 214, 443
MalI GATC 7 cut(s) 19, 204, 297, 456, 663, 720, 912
MbiI CCGCTC 1 cut(s) 698
MboI GATC 7 cut(s) 17, 202, 295, 454, 661, 718, 910
MboII GAAGA 6 cut(s) 565, 668, 671, 713, 737, 920
MflI RGATCY 6 cut(s) 17, 295, 454, 661, 718, 910
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 2 cut(s) 229, 324
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 1 cut(s) 550
MmeI TCCRAC 4 cut(s) 59, 67, 69, 216
MroI TCCGGA 1 cut(s) 298
MseI TTAA 2 cut(s) 549, 690
MslI CAYNNNNRTG 1 cut(s) 823
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 4 cut(s) 104, 281, 293, 299
MspR9I CCNGG 3 cut(s) 281, 293, 470
MvaI CCWGG 1 cut(s) 470
MvnI CGCG 3 cut(s) 40, 110, 364
MwoI GCNNNNNNNGC 1 cut(s) 104
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 2 cut(s) 281, 293
NcoI CCATGG 1 cut(s) 93
NdeII GATC 7 cut(s) 17, 202, 295, 454, 661, 718, 910
NlaIII CATG 3 cut(s) 97, 715, 828
NlaIV GGNNCC 6 cut(s) 19, 91, 99, 297, 456, 857
NmuCI GTSAC 1 cut(s) 443
NruI TCGCGA 1 cut(s) 364
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 7 cut(s) 56, 112, 302, 343, 487, 511, 896
PkrI GCNGC 4 cut(s) 28, 109, 671, 684
PleI GAGTC 1 cut(s) 550
PluTI GGCGCC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 550
PpuMI RGGWCCY 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 83
Psp5II RGGWCCY 1 cut(s) 855
Psp6I CCWGG 1 cut(s) 468
PspGI CCWGG 1 cut(s) 468
PspN4I GGNNCC 6 cut(s) 19, 91, 99, 297, 456, 857
PspPI GGNCC 1 cut(s) 855
PspPPI RGGWCCY 1 cut(s) 855
PstI CTGCAG 1 cut(s) 687
PstNI CAGNNNCTG 1 cut(s) 508
PsuI RGATCY 6 cut(s) 17, 295, 454, 661, 718, 910
RruI TCGCGA 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 823
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 549, 690
SatI GCNGC 4 cut(s) 27, 108, 670, 683
Sau3AI GATC 7 cut(s) 17, 202, 295, 454, 661, 718, 910
Sau96I GGNCC 1 cut(s) 855
SchI GAGTC 1 cut(s) 550
ScrFI CCNGG 3 cut(s) 281, 293, 470
SduI GDGCHC 1 cut(s) 83
SfaNI GCATC 1 cut(s) 823
SfcI CTRYAG 2 cut(s) 537, 683
SfoI GGCGCC 1 cut(s) 99
SinI GGWCC 1 cut(s) 855
SmiMI CAYNNNNRTG 1 cut(s) 823
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
Sse9I AATT 2 cut(s) 229, 324
SsiI CCGC 3 cut(s) 26, 108, 698
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 1 cut(s) 890
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 3 cut(s) 279, 291, 468
StyI CCWWGG 2 cut(s) 93, 859
TaqI TCGA 1 cut(s) 305
TaqII GACCGA 1 cut(s) 115
TasI AATT 2 cut(s) 229, 324
TauI GCSGC 2 cut(s) 29, 110
TfiI GAWTC 7 cut(s) 56, 112, 302, 343, 487, 511, 896
Tru1I TTAA 2 cut(s) 549, 690
Tru9I TTAA 2 cut(s) 549, 690
TscAI CASTG 1 cut(s) 521
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 2 cut(s) 669, 682
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 3 cut(s) 656, 727, 909
TspGWI ACGGA 5 cut(s) 73, 153, 467, 708, 865
TspRI CASTG 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 855
XbaI TCTAGA 1 cut(s) 889
XmiI GTMKAC 1 cut(s) 167
XspI CTAG 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.