MD10G1314600.v1.1

dnaJ homolog subfamily C member 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013208
Physical Location & Seq
Forward (+)
39880866 .. 39883783
2918 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1314600.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGCGCCACCGGCAGCGATTCGGTGGTTCTCGGTCGTTTTAGCTCTAATGCTGTGCTATCTCATCACTCCGTCAACGGCCATATACTGCGACGAGGACGACTGCTACGATCTCCTCGGGGTTACGCAGAGCGCCAACTCGTCGGAAATCAAGAAAGCTTACTACAAACTCTCATTGAAACATCACCCGGATAAAAACCCGGATCCGGAATCGAAAAAGCTGTTTGTGAAAATTGCCAATGCCTATGAGATTCTGAAAGACGAGGCGTCGCGGGAGCAGTATGATTATGCCATTGCACATCCAGAGGAGGTATTTTACAATACTGCTCGCTACTATCAAGCATATTATGGTCACAAAACGGATCCTCGTGCTGTCCTGGTTGGCCTTCTTTTGGTTCTTTCCTCATTTCAGTATCTGAATCAGTGGACAAGGTATAATCAGGCTGTAGACATGGTTAAGAAGACCCCTGCTTACAAAAATAGACTACGGGCTTTGGAACTTGAGCGTAGTGGAGGGACTACAAATAAGAAAAAGAGCAACAGGCAGATGAACAAGAAAGTGGAAGAAGATCTCGGCAGCGAACTGGAGCTGCAGATTAATGGAGCGGAGCAACCCTCCATGTGGGATCTTCTTGGTGTTCGTTTCATACTCCTTCCCTACACTATTGGCAAGCTATTATTATGGTGTGGCTGTTGGTTCTGGAGATACAAGGTGAAAAAGGCACCATATACATGGGATGATGCCTCCTACCTGACACGAAAATCCCTTGGTGCGCCTATTGATGGATGGAGAAATCTAGATGAATCAAGAAAGGAAGATCTTATTCATAAACGTTTATGGGAGAAAGCAAACTTGCAGAGCTACCTTGCGGATATGCGCAAAGAATCCAAGCGGAGAAGATAG

Protein Analysis

301

Amino Acids

35.08

Weight (kDa)

9.15

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 34 - 95 6.3e-24 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61770
fragaria_vesca FvH4_3g02660
malus_domestica MD05G1341100.v1.1 MD10G1314600.v1.1
prunus_persica Prupe.4G026200_v2.0.a1 Prupe.4G026200_v2.0.a1
pyrus_communis pycom05g31040 pycom10g26670
rosa_chinensis RchiOBHm_Chr5g0003981
rosa_laevigata RLG00000031194
rosa_multiflora Rmu_sc0000547.1_g000013
rosa_roxburghii Rroxscaffold_1G00071980
rosa_rugosa Rorug04G0408700
rosa_samantha Rh5AG036400 Rh5BG035500 Rh5CG039100 Rh5DG035000
rosa_wichuraiana Rw5G003310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 878
AccB1I GGYRCC 2 cut(s) 4, 721
AccBSI CCGCTC 1 cut(s) 605
AccI GTMKAC 1 cut(s) 447
AccII CGCG 1 cut(s) 271
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 4 cut(s) 271, 605, 869, 892
AclI AACGTT 1 cut(s) 832
AclWI GGATC 5 cut(s) 197, 210, 356, 369, 633
AcoI YGGCCR 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 5, 266
AfiI CCNNNNNNNGG 4 cut(s) 205, 391, 621, 782
AgsI TTSAA 1 cut(s) 178
AhdI GACNNNNNGTC 1 cut(s) 265
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 6 cut(s) 44, 158, 220, 589, 673, 861
AluI AGCT 6 cut(s) 44, 158, 220, 589, 673, 861
AlwI GGATC 5 cut(s) 197, 210, 356, 369, 633
AlwNI CAGNNNCTG 1 cut(s) 415
Ama87I CYCGRG 1 cut(s) 116
Aor13HI TCCGGA 1 cut(s) 205
AoxI GGCC 2 cut(s) 78, 382
ApeKI GCWGC 3 cut(s) 14, 576, 589
AseI ATTAAT 1 cut(s) 597
AspLEI GCGC 4 cut(s) 7, 134, 775, 879
AsuC2I CCSGG 2 cut(s) 188, 200
AsuHPI GGTGA 2 cut(s) 176, 724
AvaI CYCGRG 1 cut(s) 116
BamHI GGATCC 2 cut(s) 202, 361
BanI GGYRCC 2 cut(s) 4, 721
BauI CACGAG 1 cut(s) 366
BbsI GAAGAC 1 cut(s) 467
BbvI GCAGC 3 cut(s) 26, 576, 588
BccI CCATC 2 cut(s) 776, 780
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 1 cut(s) 377
BcnI CCSGG 2 cut(s) 188, 200
BfaI CTAG 1 cut(s) 797
BfmI CTRYAG 2 cut(s) 444, 590
BfoI RGCGCY 2 cut(s) 8, 135
BglII AGATCT 2 cut(s) 568, 817
BisI GCNGC 3 cut(s) 15, 577, 590
BlsI GCNGC 3 cut(s) 16, 578, 591
Bme1390I CCNGG 3 cut(s) 188, 200, 377
BmeRI GACNNNNNGTC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 116
BmiI GGNNCC 4 cut(s) 6, 204, 363, 723
BmrFI CCNGG 3 cut(s) 188, 200, 377
BmsI GCATC 1 cut(s) 730
BpiI GAAGAC 1 cut(s) 467
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BpmI CTGGAG 2 cut(s) 605, 721
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 2 cut(s) 188, 200
BsaHI GRCGYC 2 cut(s) 5, 266
BsaJI CCNNGG 2 cut(s) 115, 766
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 391, 621, 782
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 291
BseAI TCCGGA 1 cut(s) 205
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 2 cut(s) 115, 766
BseGI GGATG 3 cut(s) 298, 742, 791
BseLI CCNNNNNNNGG 4 cut(s) 205, 391, 621, 782
BseMI GCAATG 1 cut(s) 291
BseNI ACTGG 1 cut(s) 588
BseRI GAGGAG 2 cut(s) 104, 320
BseXI GCAGC 3 cut(s) 26, 576, 588
Bsh1236I CGCG 1 cut(s) 271
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 80, 384
BshNI GGYRCC 2 cut(s) 4, 721
BsiEI CGRYCG 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 4 cut(s) 11, 188, 200, 206
BslFI GGGAC 1 cut(s) 529
BslI CCNNNNNNNGG 4 cut(s) 205, 391, 621, 782
BsmFI GGGAC 1 cut(s) 529
BsnI GGCC 2 cut(s) 80, 384
BsoBI CYCGRG 1 cut(s) 116
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 6 cut(s) 109, 202, 361, 568, 625, 817
BspACI CCGC 4 cut(s) 271, 605, 869, 892
BspANI GGCC 2 cut(s) 80, 384
BspEI TCCGGA 1 cut(s) 205
BspFNI CGCG 1 cut(s) 271
BspLI GGNNCC 4 cut(s) 6, 204, 363, 723
BspMAI CTGCAG 1 cut(s) 594
BspPI GGATC 5 cut(s) 197, 210, 356, 369, 633
BspT107I GGYRCC 2 cut(s) 4, 721
BsrBI CCGCTC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 1 cut(s) 588
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 115, 766
BssMI GATC 6 cut(s) 109, 202, 361, 568, 625, 817
BssNI GRCGYC 2 cut(s) 5, 266
BssSI CACGAG 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 766
Bst2BI CACGAG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 377
BstACI GRCGYC 2 cut(s) 5, 266
BstC8I GCNNGC 2 cut(s) 328, 671
BstF5I GGATG 3 cut(s) 298, 742, 791
BstFNI CGCG 1 cut(s) 271
BstH2I RGCGCY 2 cut(s) 8, 135
BstHHI GCGC 4 cut(s) 7, 134, 775, 879
BstKTI GATC 6 cut(s) 112, 205, 364, 571, 628, 820
BstMBI GATC 6 cut(s) 109, 202, 361, 568, 625, 817
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 3 cut(s) 186, 198, 375
BstSFI CTRYAG 2 cut(s) 444, 590
BstUI CGCG 1 cut(s) 271
BstV1I GCAGC 3 cut(s) 26, 576, 588
BstV2I GAAGAC 1 cut(s) 467
BstX2I RGATCY 5 cut(s) 202, 361, 568, 625, 817
BstXI CCANNNNNNTGG 1 cut(s) 732
BstYI RGATCY 5 cut(s) 202, 361, 568, 625, 817
BsuRI GGCC 2 cut(s) 80, 384
BtsCI GGATG 3 cut(s) 298, 742, 791
BtsIMutI CAGTG 1 cut(s) 428
Cac8I GCNNGC 2 cut(s) 328, 671
CaiI CAGNNNCTG 1 cut(s) 415
CfoI GCGC 4 cut(s) 7, 134, 775, 879
Cfr10I RCCGGY 1 cut(s) 10
CseI GACGC 1 cut(s) 255
CviAII CATG 3 cut(s) 451, 619, 732
DinI GGCGCC 1 cut(s) 6
DpnI GATC 6 cut(s) 111, 204, 363, 570, 627, 819
DpnII GATC 6 cut(s) 109, 202, 361, 568, 625, 817
DriI GACNNNNNGTC 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 78
Eam1105I GACNNNNNGTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 766
Eco88I CYCGRG 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 766
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 766
FaeI CATG 3 cut(s) 454, 622, 735
FaqI GGGAC 1 cut(s) 529
FatI CATG 3 cut(s) 450, 618, 731
FauI CCCGC 1 cut(s) 264
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 3 cut(s) 15, 577, 590
FokI GGATG 3 cut(s) 285, 749, 798
Fsp4HI GCNGC 3 cut(s) 15, 577, 590
FspBI CTAG 1 cut(s) 797
FspI TGCGCA 1 cut(s) 878
GlaI GCGC 4 cut(s) 6, 133, 774, 878
GluI GCNGC 3 cut(s) 15, 577, 590
GsuI CTGGAG 2 cut(s) 605, 721
HaeII RGCGCY 2 cut(s) 8, 135
HaeIII GGCC 2 cut(s) 80, 384
HapII CCGG 4 cut(s) 11, 188, 200, 206
HgaI GACGC 1 cut(s) 255
HhaI GCGC 4 cut(s) 7, 134, 775, 879
Hin1I GRCGYC 2 cut(s) 5, 266
Hin1II CATG 3 cut(s) 454, 622, 735
Hin6I GCGC 4 cut(s) 5, 132, 773, 877
HinP1I GCGC 4 cut(s) 5, 132, 773, 877
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 6 cut(s) 19, 209, 250, 418, 803, 884
HpaII CCGG 4 cut(s) 11, 188, 200, 206
HphI GGTGA 2 cut(s) 176, 724
Hpy166II GTNNAC 3 cut(s) 75, 426, 448
Hpy188I TCNGA 3 cut(s) 145, 255, 417
Hpy188III TCNNGA 6 cut(s) 151, 206, 302, 700, 797, 807
Hpy8I GTNNAC 3 cut(s) 75, 426, 448
Hpy99I CGWCG 3 cut(s) 95, 145, 271
HpyAV CCTTC 2 cut(s) 395, 662
HpyCH4IV ACGT 1 cut(s) 832
HpyCH4V TGCA 3 cut(s) 296, 592, 856
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 832
Hsp92I GRCGYC 2 cut(s) 5, 266
Hsp92II CATG 3 cut(s) 454, 622, 735
HspAI GCGC 4 cut(s) 5, 132, 773, 877
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 205
Kzo9I GATC 6 cut(s) 109, 202, 361, 568, 625, 817
LmnI GCTCC 4 cut(s) 274, 586, 602, 607
Lsp1109I GCAGC 3 cut(s) 26, 576, 588
LweI GCATC 1 cut(s) 730
MaeI CTAG 1 cut(s) 797
MaeII ACGT 1 cut(s) 832
MaeIII GTNAC 2 cut(s) 121, 350
MalI GATC 6 cut(s) 111, 204, 363, 570, 627, 819
MbiI CCGCTC 1 cut(s) 605
MboI GATC 6 cut(s) 109, 202, 361, 568, 625, 817
MboII GAAGA 5 cut(s) 472, 575, 578, 620, 827
MflI RGATCY 5 cut(s) 202, 361, 568, 625, 817
MluCI AATT 1 cut(s) 231
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 123
MroI TCCGGA 1 cut(s) 205
MseI TTAA 2 cut(s) 456, 597
MslI CAYNNNNRTG 1 cut(s) 730
MspI CCGG 4 cut(s) 11, 188, 200, 206
MspR9I CCNGG 3 cut(s) 188, 200, 377
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 1 cut(s) 271
MwoI GCNNNNNNNGC 1 cut(s) 11
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 188, 200
NdeII GATC 6 cut(s) 109, 202, 361, 568, 625, 817
NlaIII CATG 3 cut(s) 454, 622, 735
NlaIV GGNNCC 4 cut(s) 6, 204, 363, 723
NmeAIII GCCGAG 1 cut(s) 552
NmuCI GTSAC 1 cut(s) 350
NsbI TGCGCA 1 cut(s) 878
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 6 cut(s) 19, 209, 250, 418, 803, 884
PkrI GCNGC 3 cut(s) 16, 578, 591
PluTI GGCGCC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 597
Psp1406I AACGTT 1 cut(s) 832
Psp6I CCWGG 1 cut(s) 375
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 4 cut(s) 6, 204, 363, 723
PstI CTGCAG 1 cut(s) 594
PstNI CAGNNNCTG 1 cut(s) 415
PsuI RGATCY 5 cut(s) 202, 361, 568, 625, 817
RseI CAYNNNNRTG 1 cut(s) 730
SaqAI TTAA 2 cut(s) 456, 597
SatI GCNGC 3 cut(s) 15, 577, 590
Sau3AI GATC 6 cut(s) 109, 202, 361, 568, 625, 817
ScrFI CCNGG 3 cut(s) 188, 200, 377
SfaNI GCATC 1 cut(s) 730
SfcI CTRYAG 2 cut(s) 444, 590
SfoI GGCGCC 1 cut(s) 6
SmiMI CAYNNNNRTG 1 cut(s) 730
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
Sse9I AATT 1 cut(s) 231
SsiI CCGC 4 cut(s) 271, 605, 869, 892
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 797
StyD4I CCNGG 3 cut(s) 186, 198, 375
StyI CCWWGG 1 cut(s) 766
TaiI ACGT 1 cut(s) 835
TaqI TCGA 1 cut(s) 212
TaqII GACCGA 1 cut(s) 22
TasI AATT 1 cut(s) 231
TfiI GAWTC 6 cut(s) 19, 209, 250, 418, 803, 884
Tru1I TTAA 2 cut(s) 456, 597
Tru9I TTAA 2 cut(s) 456, 597
TscAI CASTG 1 cut(s) 428
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 3 cut(s) 14, 576, 589
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 4 cut(s) 563, 634, 815, 816
TspGWI ACGGA 2 cut(s) 60, 374
TspRI CASTG 1 cut(s) 428
VspI ATTAAT 1 cut(s) 597
XbaI TCTAGA 1 cut(s) 796
XmiI GTMKAC 1 cut(s) 447
XspI CTAG 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.