MD05G1341100.v1.1

dnaJ homolog subfamily C member 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013208
Physical Location & Seq
Forward (+)
46176484 .. 46180341
3858 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1341100.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGCTATCTTCCGAATATCATATCACCACGGGACCATTTATCCGAGGCTTTTTATTTTTTATATTTTATTTTGGTTGTCATTAAACGCCCTCCAACTCTCGGAATCCATTTTCAGTGAAATCCGGAGCCCCGTCGGAACCATGGCGCCACCGGCAGCGATTCGGTGGTTGGCGGTTGTTTCATCTCTATTGCTGTTCCAGCTCATCACTCCATCGACGGCCATCTACTGCGATGAGGACGACTGCTACGATCTCCTCGGGGTTACGCAGAGCGCCAATTCGTCGGAAATCAAGAAAGCTTACTACAAGCTCTCATTGAAACATCACCCGGATAAAAACCCGGATCCGGAATCGAAAAAGCTGTTTGTGAAAATTGCCAACGCTTATGAGATTCTGAAAGACGAAGCGTCGCGGGAGCAGTATGATTATGCCATTGCACATCCAGAGGAGGTATTTTACAATACGGCTCGCTACTATCAAGCATATTATGGTCACAAAACGGATCCTCGGGCTGTCCTGGTTGGCCTTCTTTTGATTCTTTCTGCATTTCAGTATCTAAATCAGTGGACAAGGTATAATCAGGCTGTAGACATGGTCAAGAAGACTCCTGCGTACAAAAATAGACTACGGGCTTTGGAACTTGAGCGTACTGGAGGGACTACAAATAAAAAGAAGAGCAACAGGCAGATGAACAAGAAAGTGGAAGAAGATCTCGGCAGCGAACTGGAGCTGCAGATTAACGGTGCGGAACAACCCTCCATGTGGGATCTTCTTGGTGTCCGTTTCATACTTCTGCCCTACACTATTGGCAAGCTATTACTATGGTGTGGCTGTTGGTTCTGGAGATACAATGTGAGAAAAGCACCATATACATGGGATGATGCCTCCTACCTGACACGGAGATCCCTTGGTGCGCCTATTGACGGATGGAGAAATCTAGATGAATCAAGAAAGGAAGATCTTATTCATAAACGTTTATGGGAAAAAGCAAACTTGGAGAGCTACCTTGCGGATATGCGCAAAGAATCCAAGCGGAGAAGATAG

Protein Analysis

348

Amino Acids

40.6

Weight (kDa)

9.13

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 81 - 142 8e-24 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61770
fragaria_vesca FvH4_3g02660
malus_domestica MD05G1341100.v1.1 MD10G1314600.v1.1
prunus_persica Prupe.4G026200_v2.0.a1 Prupe.4G026200_v2.0.a1
pyrus_communis pycom05g31040 pycom10g26670
rosa_chinensis RchiOBHm_Chr5g0003981
rosa_laevigata RLG00000031194
rosa_multiflora Rmu_sc0000547.1_g000013
rosa_roxburghii Rroxscaffold_1G00071980
rosa_rugosa Rorug04G0408700
rosa_samantha Rh5AG036400 Rh5BG035500 Rh5CG039100 Rh5DG035000
rosa_wichuraiana Rw5G003310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1019
AccB1I GGYRCC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 588
AccII CGCG 1 cut(s) 412
AccIII TCCGGA 2 cut(s) 123, 346
AciI CCGC 5 cut(s) 173, 412, 746, 1010, 1033
AclI AACGTT 1 cut(s) 973
AclWI GGATC 6 cut(s) 338, 351, 497, 510, 774, 897
AcoI YGGCCR 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 2 cut(s) 614, 649
AfiI CCNNNNNNNGG 4 cut(s) 100, 346, 762, 923
AgsI TTSAA 1 cut(s) 319
AhdI GACNNNNNGTC 1 cut(s) 406
AjnI CCWGG 1 cut(s) 516
AluBI AGCT 7 cut(s) 202, 299, 310, 361, 730, 814, 1002
AluI AGCT 7 cut(s) 202, 299, 310, 361, 730, 814, 1002
AlwI GGATC 6 cut(s) 338, 351, 497, 510, 774, 897
Ama87I CYCGRG 2 cut(s) 257, 507
Aor13HI TCCGGA 2 cut(s) 123, 346
AoxI GGCC 2 cut(s) 219, 523
ApeKI GCWGC 3 cut(s) 155, 717, 730
AspLEI GCGC 4 cut(s) 148, 275, 916, 1020
AspS9I GGNCC 1 cut(s) 33
AsuC2I CCSGG 2 cut(s) 329, 341
AsuHPI GGTGA 2 cut(s) 17, 317
AvaI CYCGRG 2 cut(s) 257, 507
AvaII GGWCC 1 cut(s) 33
BamHI GGATCC 2 cut(s) 343, 502
BanI GGYRCC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 608
BbvI GCAGC 3 cut(s) 167, 717, 729
BccI CCATC 3 cut(s) 220, 230, 921
BceAI ACGGC 2 cut(s) 234, 480
BciT130I CCWGG 1 cut(s) 518
BcnI CCSGG 2 cut(s) 329, 341
BfaI CTAG 1 cut(s) 938
BfmI CTRYAG 2 cut(s) 585, 731
BfoI RGCGCY 2 cut(s) 149, 276
BglII AGATCT 2 cut(s) 709, 958
BisI GCNGC 3 cut(s) 156, 718, 731
BlsI GCNGC 3 cut(s) 157, 719, 732
Bme1390I CCNGG 3 cut(s) 329, 341, 518
Bme18I GGWCC 1 cut(s) 33
BmeRI GACNNNNNGTC 1 cut(s) 406
BmeT110I CYCGRG 2 cut(s) 257, 507
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 6 cut(s) 34, 128, 139, 147, 345, 504
BmrFI CCNGG 3 cut(s) 329, 341, 518
BmsI GCATC 1 cut(s) 871
BpiI GAAGAC 1 cut(s) 608
BpmI CTGGAG 3 cut(s) 672, 746, 862
BpuEI CTTGAG 1 cut(s) 662
BpuMI CCSGG 2 cut(s) 329, 341
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 6 cut(s) 28, 44, 141, 256, 506, 907
BsaWI WCCGGW 2 cut(s) 123, 346
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 346, 762, 923
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 2 cut(s) 655, 729
Bse3DI GCAATG 1 cut(s) 432
BseAI TCCGGA 2 cut(s) 123, 346
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 6 cut(s) 28, 44, 141, 256, 506, 907
BseGI GGATG 3 cut(s) 439, 883, 932
BseLI CCNNNNNNNGG 4 cut(s) 100, 346, 762, 923
BseMI GCAATG 1 cut(s) 432
BseNI ACTGG 2 cut(s) 655, 729
BseRI GAGGAG 2 cut(s) 245, 461
BseXI GCAGC 3 cut(s) 167, 717, 729
Bsh1236I CGCG 1 cut(s) 412
BshFI GGCC 2 cut(s) 221, 525
BshNI GGYRCC 1 cut(s) 145
BsiHKCI CYCGRG 2 cut(s) 257, 507
BsiSI CCGG 5 cut(s) 124, 152, 329, 341, 347
BslFI GGGAC 2 cut(s) 46, 670
BslI CCNNNNNNNGG 4 cut(s) 100, 346, 762, 923
BsmFI GGGAC 2 cut(s) 46, 670
BsnI GGCC 2 cut(s) 221, 525
BsoBI CYCGRG 2 cut(s) 257, 507
Bsp1286I GDGCHC 1 cut(s) 131
Bsp13I TCCGGA 2 cut(s) 123, 346
Bsp143I GATC 7 cut(s) 250, 343, 502, 709, 766, 902, 958
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 5 cut(s) 173, 412, 746, 1010, 1033
BspANI GGCC 2 cut(s) 221, 525
BspEI TCCGGA 2 cut(s) 123, 346
BspFNI CGCG 1 cut(s) 412
BspLI GGNNCC 6 cut(s) 34, 128, 139, 147, 345, 504
BspMAI CTGCAG 1 cut(s) 735
BspPI GGATC 6 cut(s) 338, 351, 497, 510, 774, 897
BspQI GCTCTTC 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 151
BsrI ACTGG 2 cut(s) 655, 729
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 6 cut(s) 28, 44, 141, 256, 506, 907
BssMI GATC 7 cut(s) 250, 343, 502, 709, 766, 902, 958
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 141, 907
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 1 cut(s) 743
Bst6I CTCTTC 1 cut(s) 668
BstACI GRCGYC 1 cut(s) 146
BstC8I GCNNGC 2 cut(s) 469, 812
BstDSI CCRYGG 2 cut(s) 28, 141
BstF5I GGATG 3 cut(s) 439, 883, 932
BstFNI CGCG 1 cut(s) 412
BstH2I RGCGCY 2 cut(s) 149, 276
BstHHI GCGC 4 cut(s) 148, 275, 916, 1020
BstKTI GATC 7 cut(s) 253, 346, 505, 712, 769, 905, 961
BstMBI GATC 7 cut(s) 250, 343, 502, 709, 766, 902, 958
BstMWI GCNNNNNNNGC 2 cut(s) 152, 199
BstNI CCWGG 1 cut(s) 518
BstSCI CCNGG 3 cut(s) 327, 339, 516
BstSFI CTRYAG 2 cut(s) 585, 731
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 3 cut(s) 167, 717, 729
BstV2I GAAGAC 1 cut(s) 608
BstX2I RGATCY 6 cut(s) 343, 502, 709, 766, 902, 958
BstXI CCANNNNNNTGG 1 cut(s) 873
BstYI RGATCY 6 cut(s) 343, 502, 709, 766, 902, 958
BsuRI GGCC 2 cut(s) 221, 525
BtgI CCRYGG 2 cut(s) 28, 141
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 3 cut(s) 439, 883, 932
BtsIMutI CAGTG 2 cut(s) 121, 569
Cac8I GCNNGC 2 cut(s) 469, 812
CfoI GCGC 4 cut(s) 148, 275, 916, 1020
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 33
CseI GACGC 1 cut(s) 396
Csp6I GTAC 2 cut(s) 613, 648
CviAII CATG 4 cut(s) 142, 592, 760, 873
CviQI GTAC 2 cut(s) 613, 648
DinI GGCGCC 1 cut(s) 147
DpnI GATC 7 cut(s) 252, 345, 504, 711, 768, 904, 960
DpnII GATC 7 cut(s) 250, 343, 502, 709, 766, 902, 958
DriI GACNNNNNGTC 1 cut(s) 406
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 668
Eam1105I GACNNNNNGTC 1 cut(s) 406
EarI CTCTTC 1 cut(s) 668
Eco130I CCWWGG 2 cut(s) 141, 907
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 33
Eco88I CYCGRG 2 cut(s) 257, 507
EcoRII CCWGG 1 cut(s) 516
EcoT14I CCWWGG 2 cut(s) 141, 907
EcoT38I GRGCYC 1 cut(s) 131
EgeI GGCGCC 1 cut(s) 147
EheI GGCGCC 1 cut(s) 147
ErhI CCWWGG 2 cut(s) 141, 907
FaeI CATG 4 cut(s) 145, 595, 763, 876
FaqI GGGAC 2 cut(s) 46, 670
FatI CATG 4 cut(s) 141, 591, 759, 872
FauI CCCGC 1 cut(s) 405
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 3 cut(s) 156, 718, 731
FokI GGATG 3 cut(s) 426, 890, 939
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 156, 718, 731
FspBI CTAG 1 cut(s) 938
FspI TGCGCA 1 cut(s) 1019
GlaI GCGC 4 cut(s) 147, 274, 915, 1019
GluI GCNGC 3 cut(s) 156, 718, 731
GsuI CTGGAG 3 cut(s) 672, 746, 862
HaeII RGCGCY 2 cut(s) 149, 276
HaeIII GGCC 2 cut(s) 221, 525
HapII CCGG 5 cut(s) 124, 152, 329, 341, 347
HgaI GACGC 1 cut(s) 396
HhaI GCGC 4 cut(s) 148, 275, 916, 1020
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 4 cut(s) 145, 595, 763, 876
Hin6I GCGC 4 cut(s) 146, 273, 914, 1018
HinP1I GCGC 4 cut(s) 146, 273, 914, 1018
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 8 cut(s) 104, 160, 350, 391, 535, 604, 944, 1025
HpaII CCGG 5 cut(s) 124, 152, 329, 341, 347
HphI GGTGA 2 cut(s) 17, 317
Hpy166II GTNNAC 2 cut(s) 567, 589
Hpy188I TCNGA 6 cut(s) 14, 45, 103, 137, 286, 396
Hpy188III TCNNGA 8 cut(s) 124, 292, 347, 443, 598, 841, 938, 948
Hpy8I GTNNAC 2 cut(s) 567, 589
Hpy99I CGWCG 4 cut(s) 137, 220, 286, 412
HpyAV CCTTC 1 cut(s) 536
HpyCH4III ACNGT 1 cut(s) 743
HpyCH4IV ACGT 1 cut(s) 973
HpyCH4V TGCA 3 cut(s) 437, 545, 733
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 199
HpySE526I ACGT 1 cut(s) 973
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 4 cut(s) 145, 595, 763, 876
HspAI GCGC 4 cut(s) 146, 273, 914, 1018
KasI GGCGCC 1 cut(s) 145
Kpn2I TCCGGA 2 cut(s) 123, 346
Kzo9I GATC 7 cut(s) 250, 343, 502, 709, 766, 902, 958
LguI GCTCTTC 1 cut(s) 668
LmnI GCTCC 3 cut(s) 126, 415, 727
Lsp1109I GCAGC 3 cut(s) 167, 717, 729
LweI GCATC 1 cut(s) 871
MaeI CTAG 1 cut(s) 938
MaeII ACGT 1 cut(s) 973
MaeIII GTNAC 2 cut(s) 262, 491
MalI GATC 7 cut(s) 252, 345, 504, 711, 768, 904, 960
MboI GATC 7 cut(s) 250, 343, 502, 709, 766, 902, 958
MboII GAAGA 6 cut(s) 613, 685, 716, 719, 761, 968
MflI RGATCY 6 cut(s) 343, 502, 709, 766, 902, 958
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 2 cut(s) 277, 372
Mly113I GGCGCC 1 cut(s) 146
MlyI GAGTC 1 cut(s) 598
MmeI TCCRAC 3 cut(s) 115, 118, 264
MroI TCCGGA 2 cut(s) 123, 346
MseI TTAA 2 cut(s) 83, 738
MslI CAYNNNNRTG 1 cut(s) 871
MspI CCGG 5 cut(s) 124, 152, 329, 341, 347
MspR9I CCNGG 3 cut(s) 329, 341, 518
MvaI CCWGG 1 cut(s) 518
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 2 cut(s) 152, 199
NarI GGCGCC 1 cut(s) 146
NciI CCSGG 2 cut(s) 329, 341
NcoI CCATGG 1 cut(s) 141
NdeII GATC 7 cut(s) 250, 343, 502, 709, 766, 902, 958
NlaIII CATG 4 cut(s) 145, 595, 763, 876
NlaIV GGNNCC 6 cut(s) 34, 128, 139, 147, 345, 504
NmeAIII GCCGAG 1 cut(s) 693
NmuCI GTSAC 1 cut(s) 491
NsbI TGCGCA 1 cut(s) 1019
PciSI GCTCTTC 1 cut(s) 668
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PfeI GAWTC 7 cut(s) 104, 160, 350, 391, 535, 944, 1025
PflFI GACNNNGTC 1 cut(s) 593
PkrI GCNGC 3 cut(s) 157, 719, 732
PleI GAGTC 1 cut(s) 598
PluTI GGCGCC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 598
Psp1406I AACGTT 1 cut(s) 973
Psp6I CCWGG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 6 cut(s) 34, 128, 139, 147, 345, 504
PspPI GGNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 735
PsuI RGATCY 6 cut(s) 343, 502, 709, 766, 902, 958
PsyI GACNNNGTC 1 cut(s) 593
RsaI GTAC 2 cut(s) 614, 649
RsaNI GTAC 2 cut(s) 613, 648
RseI CAYNNNNRTG 1 cut(s) 871
SapI GCTCTTC 1 cut(s) 668
SaqAI TTAA 2 cut(s) 83, 738
SatI GCNGC 3 cut(s) 156, 718, 731
Sau3AI GATC 7 cut(s) 250, 343, 502, 709, 766, 902, 958
Sau96I GGNCC 1 cut(s) 33
SchI GAGTC 1 cut(s) 598
ScrFI CCNGG 3 cut(s) 329, 341, 518
SduI GDGCHC 1 cut(s) 131
SfaNI GCATC 1 cut(s) 871
SfcI CTRYAG 2 cut(s) 585, 731
SfoI GGCGCC 1 cut(s) 147
SinI GGWCC 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 871
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 2 cut(s) 277, 372
SsiI CCGC 5 cut(s) 173, 412, 746, 1010, 1033
SspDI GGCGCC 1 cut(s) 145
SspMI CTAG 1 cut(s) 938
StyD4I CCNGG 3 cut(s) 327, 339, 516
StyI CCWWGG 2 cut(s) 141, 907
TaaI ACNGT 1 cut(s) 743
TaiI ACGT 1 cut(s) 976
TaqI TCGA 2 cut(s) 215, 353
TasI AATT 2 cut(s) 277, 372
TfiI GAWTC 7 cut(s) 104, 160, 350, 391, 535, 944, 1025
Tru1I TTAA 2 cut(s) 83, 738
Tru9I TTAA 2 cut(s) 83, 738
TscAI CASTG 2 cut(s) 121, 569
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 3 cut(s) 155, 717, 730
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 5 cut(s) 171, 704, 775, 956, 957
TspGWI ACGGA 4 cut(s) 515, 770, 913, 939
TspRI CASTG 2 cut(s) 121, 569
Tth111I GACNNNGTC 1 cut(s) 593
VpaK11BI GGWCC 1 cut(s) 33
XbaI TCTAGA 1 cut(s) 937
XmiI GTMKAC 1 cut(s) 588
XspI CTAG 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.