FvH4_3g04840

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013811
Physical Location & Seq
Forward (+)
2796193 .. 2798613
2421 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04840.t1

Sequence Viewer

Length: 1026 bp
ATGGCTTCGTTCGACGCTTTCAGCACCGACGGCGACGATCTCAACGCCCCATCCAACCGTCCATTTGACCACGACGAGGTCGTCGTCGAGGAGGAGGAGAGCTACGAGTACGGCTCCTACTCTGACTTCTCCGGCGGCCAAGGAGGAGGTGGAGTGTCGGTGGATCACGCCACCGCCGCGGAAGCCTCGCCGTTCGGATTCGAAGATCCGAGCCCGGACTACTCCGGCTCGTCGCCGTTCGCGTCGGCGGCTCCGGCTGAGAACGGGAATGGATATGGAATCGGCGAGAACGGAATCGACGACGGCGTTTTCGCGTCGGACGGGCCTGTGCTGCCGGAGCCGAGCGAGATGGTGGAGGAAGGGGTTGCTCTTCGTGAGTGGAGGAGGCAGAACGCCCTTCTGCTTGAGGAGAAGGAAAGGAGGGAGAAAGAACTGAGGATCCAGATTATTGAAGAAGCTGAGGAGTTCAAACGCGCATTTTATGAGAAAAGGAAGCTTAATGTTGAGACTAACAAGGTTCAGAACAGGGAAAAGGAGAAGTTGTTCTTGGCTGATCAAGAGAAGTTCCACAAGAATGCAGACAAGCAAAGTTGGAAGGCAATAGCTGAGCTCATTCCACACGAGATTCCCAACATCGAGAAAAAGAGAGGCAAGAAGGATCAGGACAAGAAACCATCGATCACAGTCATTCAGGGTCCAAAGCCTGGAAAACCCACTGATCTTTCAAGGATGCGACAAATACTTTTGAAGCTGAAGCATACAACTCCACCTCACATGATTCCTAAACCACCTGCTCCTGCTAAAGATGCTAAAGATGGGAAGGATGCAAAAGATCCCAAGAATGTAAAGGATGCAAAAGATCCCAAGAATGTAAAGGATGTGAAGAATGGATCTGCATCTAAAACTAATGGATCTGCAGAAGCTGTGGCGCCTGCTGCTTCAGAAACCAAAGAAGCCACCACTAATGGCACTTCAGATGCACCAGAACAAGCAGCTCCTGTTTCTACCGAGGAACAGTCTGCTTAA

Protein Analysis

342

Amino Acids

37.17

Weight (kDa)

5.02

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_lg_ch PF01086 116 - 254 5.4e-09 Clathrin light chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013811)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g04840
malus_domestica MD05G1316400.v1.1 MD10G1296900.v1.1
prunus_persica Prupe.4G043600_v2.0.a1
pyrus_communis pycom10g24980
rosa_chinensis RchiOBHm_Chr5g0008001
rosa_laevigata RLG00000031526
rosa_multiflora Rmu_co8429453.1_g000001 Rmu_co8446221.1_g000001 Rmu_sc0007348.1_g000005
rosa_roxburghii Rroxscaffold_1G00068470
rosa_rugosa Rorug04G0432300
rosa_samantha Rh5AG060500 Rh5BG057300 Rh5CG067400 Rh5DG056500
rosa_wichuraiana Rw5G006010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 799
AasI GACNNNNNNGTC 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 799
AccB1I GGYRCC 1 cut(s) 928
AccB7I CCANNNNNTGG 1 cut(s) 704
AccII CGCG 4 cut(s) 179, 242, 314, 474
AciI CCGC 5 cut(s) 135, 174, 177, 179, 248
AclWI GGATC 9 cut(s) 171, 200, 433, 446, 666, 827, 854, 898, 919
AcoI YGGCCR 1 cut(s) 136
AcuI CTGAAG 3 cut(s) 773, 924, 957
AcyI GRCGYC 1 cut(s) 929
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 76, 704
AgsI TTSAA 4 cut(s) 452, 469, 726, 748
AjnI CCWGG 1 cut(s) 703
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AloI GAACNNNNNNTCC 4 cut(s) 423, 455, 527, 559
AluBI AGCT 8 cut(s) 102, 458, 496, 605, 610, 751, 923, 995
AluI AGCT 8 cut(s) 102, 458, 496, 605, 610, 751, 923, 995
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 9 cut(s) 171, 200, 433, 446, 666, 827, 854, 898, 919
AlwNI CAGNNNCTG 2 cut(s) 923, 998
AoxI GGCC 2 cut(s) 136, 323
ApeKI GCWGC 3 cut(s) 331, 935, 992
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
Asp700I GAANNNNTTC 1 cut(s) 542
AspLEI GCGC 2 cut(s) 476, 931
AspS9I GGNCC 2 cut(s) 323, 695
AsuC2I CCSGG 1 cut(s) 215
AsuII TTCGAA 1 cut(s) 201
AvaII GGWCC 1 cut(s) 695
BamHI GGATCC 1 cut(s) 438
BanI GGYRCC 1 cut(s) 928
BanII GRGCYC 2 cut(s) 215, 612
BauI CACGAG 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 612
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 3 cut(s) 318, 922, 1004
BccI CCATC 4 cut(s) 58, 343, 682, 809
BceAI ACGGC 5 cut(s) 46, 127, 175, 220, 319
BciT130I CCWGG 1 cut(s) 705
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 1 cut(s) 500
BfmI CTRYAG 1 cut(s) 915
BfoI RGCGCY 1 cut(s) 932
BfuAI ACCTGC 1 cut(s) 799
BisI GCNGC 6 cut(s) 136, 177, 249, 332, 936, 993
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 6 cut(s) 137, 178, 250, 333, 937, 994
Bme1390I CCNGG 2 cut(s) 215, 705
Bme18I GGWCC 1 cut(s) 695
BmgT120I GGNCC 2 cut(s) 323, 695
BmiI GGNNCC 6 cut(s) 115, 252, 339, 440, 696, 930
BmrFI CCNGG 2 cut(s) 215, 705
BmsI GCATC 6 cut(s) 720, 796, 814, 841, 905, 967
BplI GAGNNNNNCTC 2 cut(s) 98, 130
Bpu10I CCTNAGC 1 cut(s) 459
Bpu1102I GCTNAGC 1 cut(s) 606
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 215
Bsa29I ATCGAT 1 cut(s) 677
BsaBI GATNNNNATC 1 cut(s) 895
BsaHI GRCGYC 1 cut(s) 929
BsaJI CCNNGG 3 cut(s) 139, 177, 1008
BsaXI ACNNNNNCTCC 6 cut(s) 138, 144, 168, 174, 527, 557
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 704
Bse8I GATNNNNATC 1 cut(s) 895
BseBI CCWGG 1 cut(s) 705
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 3 cut(s) 139, 177, 1008
BseGI GGATG 5 cut(s) 50, 735, 829, 856, 883
BseJI GATNNNNATC 1 cut(s) 895
BseLI CCNNNNNNNGG 2 cut(s) 76, 704
BseMII CTCAG 4 cut(s) 249, 425, 450, 597
BseRI GAGGAG 7 cut(s) 104, 107, 110, 159, 397, 422, 476
BseXI GCAGC 3 cut(s) 318, 922, 1004
Bsh1236I CGCG 4 cut(s) 179, 242, 314, 474
BshFI GGCC 2 cut(s) 138, 325
BshNI GGYRCC 1 cut(s) 928
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 5 cut(s) 132, 215, 225, 254, 335
BslI CCNNNNNNNGG 2 cut(s) 76, 704
BsmAI GTCTC 1 cut(s) 500
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 2 cut(s) 138, 325
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 215, 612
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 5 cut(s) 135, 174, 177, 179, 248
BspANI GGCC 2 cut(s) 138, 325
BspCNI CTCAG 4 cut(s) 250, 426, 451, 598
BspDI ATCGAT 1 cut(s) 677
BspFNI CGCG 4 cut(s) 179, 242, 314, 474
BspLI GGNNCC 6 cut(s) 115, 252, 339, 440, 696, 930
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 799
BspPI GGATC 9 cut(s) 171, 200, 433, 446, 666, 827, 854, 898, 919
BspQI GCTCTTC 1 cut(s) 375
BspT104I TTCGAA 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 928
BssECI CCNNGG 3 cut(s) 139, 177, 1008
BssNI GRCGYC 1 cut(s) 929
BssSI CACGAG 1 cut(s) 620
BssT1I CCWWGG 1 cut(s) 139
Bst2BI CACGAG 1 cut(s) 620
Bst2UI CCWGG 1 cut(s) 705
Bst4CI ACNGT 3 cut(s) 59, 685, 1017
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 1 cut(s) 929
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 933
BstDEI CTNAG 4 cut(s) 258, 434, 459, 606
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 5 cut(s) 50, 735, 829, 856, 883
BstFNI CGCG 4 cut(s) 179, 242, 314, 474
BstH2I RGCGCY 1 cut(s) 932
BstHHI GCGC 2 cut(s) 476, 931
BstMAI GTCTC 1 cut(s) 500
BstMWI GCNNNNNNNGC 9 cut(s) 30, 176, 182, 248, 254, 331, 337, 806, 935
BstNI CCWGG 1 cut(s) 705
BstSCI CCNGG 2 cut(s) 213, 703
BstSFI CTRYAG 1 cut(s) 915
BstUI CGCG 4 cut(s) 179, 242, 314, 474
BstV1I GCAGC 3 cut(s) 318, 922, 1004
BstX2I RGATCY 6 cut(s) 205, 438, 832, 859, 890, 911
BstYI RGATCY 6 cut(s) 205, 438, 832, 859, 890, 911
Bsu15I ATCGAT 1 cut(s) 677
BsuRI GGCC 2 cut(s) 138, 325
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 5 cut(s) 50, 735, 829, 856, 883
BtsIMutI CAGTG 1 cut(s) 714
BveI ACCTGC 1 cut(s) 799
Cac8I GCNNGC 1 cut(s) 933
CaiI CAGNNNCTG 2 cut(s) 923, 998
CfoI GCGC 2 cut(s) 476, 931
Cfr13I GGNCC 2 cut(s) 323, 695
Cfr42I CCGCGG 1 cut(s) 180
ClaI ATCGAT 1 cut(s) 677
CseI GACGC 3 cut(s) 23, 231, 303
Csp6I GTAC 1 cut(s) 109
CviAII CATG 1 cut(s) 775
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 4 cut(s) 258, 434, 459, 606
DinI GGCGCC 1 cut(s) 930
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Ecl136II GAGCTC 1 cut(s) 610
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 2 cut(s) 215, 612
Eco47I GGWCC 1 cut(s) 695
Eco53kI GAGCTC 1 cut(s) 610
Eco57I CTGAAG 3 cut(s) 773, 924, 957
EcoICRI GAGCTC 1 cut(s) 610
EcoRII CCWGG 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 2 cut(s) 215, 612
EgeI GGCGCC 1 cut(s) 930
EheI GGCGCC 1 cut(s) 930
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 1 cut(s) 778
FaiI YATR 4 cut(s) 276, 483, 759, 776
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FatI CATG 1 cut(s) 774
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 6 cut(s) 136, 177, 249, 332, 936, 993
FokI GGATG 5 cut(s) 37, 742, 836, 863, 890
FriOI GRGCYC 2 cut(s) 215, 612
Fsp4HI GCNGC 6 cut(s) 136, 177, 249, 332, 936, 993
GlaI GCGC 2 cut(s) 475, 930
GluI GCNGC 6 cut(s) 136, 177, 249, 332, 936, 993
HaeII RGCGCY 1 cut(s) 932
HaeIII GGCC 2 cut(s) 138, 325
HapII CCGG 5 cut(s) 132, 215, 225, 254, 335
HgaI GACGC 3 cut(s) 23, 231, 303
HhaI GCGC 2 cut(s) 476, 931
Hin1I GRCGYC 1 cut(s) 929
Hin1II CATG 1 cut(s) 778
Hin6I GCGC 2 cut(s) 474, 929
HinP1I GCGC 2 cut(s) 474, 929
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 5 cut(s) 198, 279, 294, 625, 778
HpaII CCGG 5 cut(s) 132, 215, 225, 254, 335
Hpy188I TCNGA 7 cut(s) 124, 197, 210, 319, 522, 943, 976
Hpy188III TCNNGA 5 cut(s) 374, 442, 557, 637, 662
HpyAV CCTTC 6 cut(s) 353, 406, 407, 589, 649, 814
HpyCH4III ACNGT 3 cut(s) 59, 685, 1017
HpyCH4V TGCA 6 cut(s) 578, 827, 854, 896, 917, 980
HpyF10VI GCNNNNNNNGC 9 cut(s) 30, 176, 182, 248, 254, 331, 337, 806, 935
HpyF3I CTNAG 4 cut(s) 258, 434, 459, 606
Hsp92I GRCGYC 1 cut(s) 929
Hsp92II CATG 1 cut(s) 778
HspAI GCGC 2 cut(s) 474, 929
KasI GGCGCC 1 cut(s) 928
Ksp22I TGATCA 1 cut(s) 553
KspI CCGCGG 1 cut(s) 180
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 5 cut(s) 119, 256, 337, 799, 1000
Lsp1109I GCAGC 3 cut(s) 318, 922, 1004
LweI GCATC 6 cut(s) 720, 796, 814, 841, 905, 967
MboII GAAGA 4 cut(s) 215, 362, 464, 895
MflI RGATCY 6 cut(s) 205, 438, 832, 859, 890, 911
MhlI GDGCHC 2 cut(s) 215, 612
Mly113I GGCGCC 1 cut(s) 929
MmeI TCCRAC 3 cut(s) 78, 297, 572
MroXI GAANNNNTTC 1 cut(s) 542
MseI TTAA 2 cut(s) 498, 1024
MslI CAYNNNNRTG 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 5 cut(s) 132, 215, 225, 254, 335
MspR9I CCNGG 2 cut(s) 215, 705
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 1 cut(s) 705
MvnI CGCG 4 cut(s) 179, 242, 314, 474
MwoI GCNNNNNNNGC 9 cut(s) 30, 176, 182, 248, 254, 331, 337, 806, 935
NarI GGCGCC 1 cut(s) 929
NciI CCSGG 1 cut(s) 215
NlaIII CATG 1 cut(s) 778
NlaIV GGNNCC 6 cut(s) 115, 252, 339, 440, 696, 930
NmeAIII GCCGAG 1 cut(s) 366
NspV TTCGAA 1 cut(s) 201
PaqCI CACCTGC 1 cut(s) 799
PciSI GCTCTTC 1 cut(s) 375
PcsI WCGNNNNNNNCGW 5 cut(s) 78, 81, 239, 297, 303
PctI GAATGC 1 cut(s) 580
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 5 cut(s) 198, 279, 294, 625, 778
PflFI GACNNNGTC 1 cut(s) 77
PflMI CCANNNNNTGG 1 cut(s) 704
PkrI GCNGC 6 cut(s) 137, 178, 250, 333, 937, 994
PluTI GGCGCC 1 cut(s) 932
Psp124BI GAGCTC 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 703
PspGI CCWGG 1 cut(s) 703
PspN4I GGNNCC 6 cut(s) 115, 252, 339, 440, 696, 930
PspPI GGNCC 2 cut(s) 323, 695
PstI CTGCAG 1 cut(s) 919
PstNI CAGNNNCTG 2 cut(s) 923, 998
PsuI RGATCY 6 cut(s) 205, 438, 832, 859, 890, 911
PsyI GACNNNGTC 1 cut(s) 77
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 573
SacI GAGCTC 1 cut(s) 612
SacII CCGCGG 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 2 cut(s) 498, 1024
SatI GCNGC 6 cut(s) 136, 177, 249, 332, 936, 993
Sau96I GGNCC 2 cut(s) 323, 695
ScrFI CCNGG 2 cut(s) 215, 705
SduI GDGCHC 2 cut(s) 215, 612
SfaNI GCATC 6 cut(s) 720, 796, 814, 841, 905, 967
SfcI CTRYAG 1 cut(s) 915
SfoI GGCGCC 1 cut(s) 930
Sfr303I CCGCGG 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 201
SgrBI CCGCGG 1 cut(s) 180
SinI GGWCC 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SsiI CCGC 5 cut(s) 135, 174, 177, 179, 248
SspDI GGCGCC 1 cut(s) 928
SstI GAGCTC 1 cut(s) 612
StyD4I CCNGG 2 cut(s) 213, 703
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 3 cut(s) 59, 685, 1017
TaqI TCGA 6 cut(s) 12, 87, 201, 297, 636, 677
TauI GCSGC 3 cut(s) 138, 179, 251
TfiI GAWTC 5 cut(s) 198, 279, 294, 625, 778
Tru1I TTAA 2 cut(s) 498, 1024
Tru9I TTAA 2 cut(s) 498, 1024
TscAI CASTG 1 cut(s) 721
TseI GCWGC 3 cut(s) 331, 935, 992
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 1 cut(s) 721
Tth111I GACNNNGTC 1 cut(s) 77
Van91I CCANNNNNTGG 1 cut(s) 704
VpaK11BI GGWCC 1 cut(s) 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.