Rh5AG060500

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013811
Physical Location & Seq
Forward (+)
5166227 .. 5168470
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG060500.1

Sequence Viewer

Length: 984 bp
ATGGCCTCGTTCGACGCTTTCAGCATCGACGGCGACGATCTCAACGCCTCATCCAACCGTCCATTTGACCAGGACGAGGTCGTCGTCGAGAGCTACGACTACGGCTCGTACTCGGACTTCTCCGGCGGGCTTCCCGCCGAAGGAGTGTCGACGGATCAGGCGGCGGCCTCGCCGTTCGGATTCGAAGATCCGAGCCCGAACTACTCTGGCTCGTCGCCGTTCGGGGCTGCTCCGGCTGAGAACGGTAATGGATATGGAATCGGCGAGAATGGAATCGACGATGGAGTTTTTGCTTCCGACGGTCCTGTGCTGCCGGAGCCGAGCGAGATGCAGGAGGAAGGGTTTGCTCTTCGTGAGTGGAGGAGTCAAAATGCCATTCTGCTTGAGGGGAAGGAAAAGAGAGAGAAAGAACTGAGGATCCAGATTATTGAAGAAGCTGAGGAGTTCAAACGCGCATTCTATGAGAAAAGGAAGCTTAATGTTGAGACTAACAAGGTTCAGAACAGGGAAAAGGAGAAGTTGTTCTTGGCTGATCAAGAGAACTTCCACAAGAATGCAGACAAGCAAAGTTGGAAGGCAATAGCTGAGCTCATTCCTCACGAGATTCCCAACATTGAGAAAAAGAGAGGCAAGAAGGATCAGGACAAGAAACCATCGATCACGGTCATTCAGGGTCCAAAGCCTGGAAAACCCACTGATCTTTCAAGGATGCGACAAATACTTTTGAAGCTGAAGCATACAACTCCGCCTCACATGATTCCTAAACCACCTGCTCCTGCTAAAGATGCAAAAGATGGAAAGGATGCAAAAGATGCCAAGAATGTAAAGGATGCAAAAGATGTGAAGACTGCAGCATCTAAAACTAATGGATCTGCAGAAGCTGCGGTGCCTGCTGCTTCAGAAACCAAAGATGCCACCTCTAATGGCACTTCAGATGCACCGGAACAATCAGTTCCTGTTTCTACTGAGGAACAGTCTGCTTAA

Protein Analysis

327

Amino Acids

35.58

Weight (kDa)

4.96

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_lg_ch PF01086 108 - 247 1.5e-08 Clathrin light chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013811)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g04840
malus_domestica MD05G1316400.v1.1 MD10G1296900.v1.1
prunus_persica Prupe.4G043600_v2.0.a1
pyrus_communis pycom10g24980
rosa_chinensis RchiOBHm_Chr5g0008001
rosa_laevigata RLG00000031526
rosa_multiflora Rmu_co8429453.1_g000001 Rmu_co8446221.1_g000001 Rmu_sc0007348.1_g000005
rosa_roxburghii Rroxscaffold_1G00068470
rosa_rugosa Rorug04G0432300
rosa_samantha Rh5AG060500 Rh5BG057300 Rh5CG067400 Rh5DG056500
rosa_wichuraiana Rw5G006010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 778
AasI GACNNNNNNGTC 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 778
AccB1I GGYRCC 1 cut(s) 886
AccB7I CCANNNNNTGG 1 cut(s) 683
AccI GTMKAC 1 cut(s) 149
AccII CGCG 1 cut(s) 453
AciI CCGC 6 cut(s) 126, 135, 161, 164, 746, 884
AclWI GGATC 6 cut(s) 162, 182, 412, 425, 645, 877
AcuI CTGAAG 3 cut(s) 752, 882, 915
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 3 cut(s) 76, 140, 683
AgsI TTSAA 4 cut(s) 431, 448, 705, 727
AjnI CCWGG 2 cut(s) 69, 682
AjuI GAANNNNNNNTTGG 2 cut(s) 509, 541
AloI GAACNNNNNNTCC 4 cut(s) 402, 434, 506, 538
AluBI AGCT 7 cut(s) 93, 437, 475, 584, 589, 730, 881
AluI AGCT 7 cut(s) 93, 437, 475, 584, 589, 730, 881
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 6 cut(s) 162, 182, 412, 425, 645, 877
AlwNI CAGNNNCTG 2 cut(s) 881, 956
AoxI GGCC 2 cut(s) 3, 165
ApeKI GCWGC 5 cut(s) 227, 310, 851, 881, 893
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 2 cut(s) 302, 674
AsuII TTCGAA 1 cut(s) 183
AvaII GGWCC 2 cut(s) 302, 674
BamHI GGATCC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 886
BanII GRGCYC 2 cut(s) 197, 591
BarI GAAGNNNNNNTAC 2 cut(s) 101, 133
BauI CACGAG 1 cut(s) 599
BbsI GAAGAC 1 cut(s) 851
Bbv12I GWGCWC 1 cut(s) 591
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 5 cut(s) 214, 297, 863, 868, 880
BccI CCATC 3 cut(s) 275, 661, 788
BceAI ACGGC 4 cut(s) 46, 118, 157, 202
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 2 cut(s) 71, 684
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 479
BfmI CTRYAG 2 cut(s) 849, 873
BfuAI ACCTGC 1 cut(s) 778
BisI GCNGC 7 cut(s) 162, 165, 228, 311, 852, 882, 894
BlpI GCTNAGC 1 cut(s) 585
BlsI GCNGC 7 cut(s) 163, 166, 229, 312, 853, 883, 895
Bme1390I CCNGG 2 cut(s) 71, 684
Bme18I GGWCC 2 cut(s) 302, 674
BmgT120I GGNCC 2 cut(s) 302, 674
BmiI GGNNCC 4 cut(s) 318, 419, 675, 888
BmrFI CCNGG 2 cut(s) 71, 684
BpiI GAAGAC 1 cut(s) 851
Bpu10I CCTNAGC 1 cut(s) 438
Bpu1102I GCTNAGC 1 cut(s) 585
Bpu14I TTCGAA 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 404
Bsa29I ATCGAT 1 cut(s) 656
BsaWI WCCGGW 1 cut(s) 940
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 506, 536
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 140, 683
BseBI CCWGG 2 cut(s) 71, 684
BseCI ATCGAT 1 cut(s) 656
BseGI GGATG 4 cut(s) 50, 714, 808, 835
BseLI CCNNNNNNNGG 3 cut(s) 76, 140, 683
BseMII CTCAG 5 cut(s) 228, 404, 429, 576, 957
BseRI GAGGAG 2 cut(s) 376, 455
BseXI GCAGC 5 cut(s) 214, 297, 863, 868, 880
Bsh1236I CGCG 1 cut(s) 453
BshFI GGCC 2 cut(s) 5, 167
BshNI GGYRCC 1 cut(s) 886
BshVI ATCGAT 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 591
BsiSI CCGG 4 cut(s) 123, 233, 314, 941
BslI CCNNNNNNNGG 3 cut(s) 76, 140, 683
BsmAI GTCTC 1 cut(s) 479
BsmI GAATGC 2 cut(s) 455, 559
BsnI GGCC 2 cut(s) 5, 167
Bsp119I TTCGAA 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 197, 591
Bsp143I GATC 9 cut(s) 37, 154, 187, 417, 532, 637, 657, 697, 869
Bsp1720I GCTNAGC 1 cut(s) 585
BspACI CCGC 6 cut(s) 126, 135, 161, 164, 746, 884
BspANI GGCC 2 cut(s) 5, 167
BspCNI CTCAG 5 cut(s) 229, 405, 430, 577, 958
BspDI ATCGAT 1 cut(s) 656
BspFNI CGCG 1 cut(s) 453
BspLI GGNNCC 4 cut(s) 318, 419, 675, 888
BspMAI CTGCAG 2 cut(s) 853, 877
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 6 cut(s) 162, 182, 412, 425, 645, 877
BspQI GCTCTTC 1 cut(s) 354
BspT104I TTCGAA 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 886
BssMI GATC 9 cut(s) 37, 154, 187, 417, 532, 637, 657, 697, 869
BssSI CACGAG 1 cut(s) 599
Bst2BI CACGAG 1 cut(s) 599
Bst2UI CCWGG 2 cut(s) 71, 684
Bst4CI ACNGT 5 cut(s) 59, 245, 302, 664, 975
Bst6I CTCTTC 1 cut(s) 354
BstAPI GCANNNNNTGC 2 cut(s) 812, 881
BstBI TTCGAA 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 128, 891
BstDEI CTNAG 5 cut(s) 237, 413, 438, 585, 966
BstF5I GGATG 4 cut(s) 50, 714, 808, 835
BstFNI CGCG 1 cut(s) 453
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 9 cut(s) 40, 157, 190, 420, 535, 640, 660, 700, 872
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 9 cut(s) 37, 154, 187, 417, 532, 637, 657, 697, 869
BstMWI GCNNNNNNNGC 7 cut(s) 30, 233, 316, 785, 812, 881, 890
BstNI CCWGG 2 cut(s) 71, 684
BstSCI CCNGG 2 cut(s) 69, 682
BstSFI CTRYAG 2 cut(s) 849, 873
BstUI CGCG 1 cut(s) 453
BstV1I GCAGC 5 cut(s) 214, 297, 863, 868, 880
BstV2I GAAGAC 1 cut(s) 851
BstX2I RGATCY 3 cut(s) 187, 417, 869
BstYI RGATCY 3 cut(s) 187, 417, 869
Bsu15I ATCGAT 1 cut(s) 656
BsuRI GGCC 2 cut(s) 5, 167
BsuTUI ATCGAT 1 cut(s) 656
BtsCI GGATG 4 cut(s) 50, 714, 808, 835
BtsIMutI CAGTG 1 cut(s) 693
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 2 cut(s) 128, 891
CaiI CAGNNNCTG 2 cut(s) 881, 956
CfoI GCGC 1 cut(s) 455
Cfr13I GGNCC 2 cut(s) 302, 674
ClaI ATCGAT 1 cut(s) 656
CseI GACGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 109
CviAII CATG 1 cut(s) 754
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 5 cut(s) 237, 413, 438, 585, 966
DpnI GATC 9 cut(s) 39, 156, 189, 419, 534, 639, 659, 699, 871
DpnII GATC 9 cut(s) 37, 154, 187, 417, 532, 637, 657, 697, 869
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
EciI GGCGGA 1 cut(s) 735
Ecl136II GAGCTC 1 cut(s) 589
Eco24I GRGCYC 2 cut(s) 197, 591
Eco47I GGWCC 2 cut(s) 302, 674
Eco53kI GAGCTC 1 cut(s) 589
Eco57I CTGAAG 3 cut(s) 752, 882, 915
EcoICRI GAGCTC 1 cut(s) 589
EcoRII CCWGG 2 cut(s) 69, 682
EcoT38I GRGCYC 2 cut(s) 197, 591
FaeI CATG 1 cut(s) 757
FaiI YATR 4 cut(s) 255, 462, 738, 755
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FatI CATG 1 cut(s) 753
FauI CCCGC 2 cut(s) 119, 142
FbaI TGATCA 1 cut(s) 532
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 7 cut(s) 162, 165, 228, 311, 852, 882, 894
FokI GGATG 4 cut(s) 37, 721, 815, 842
FriOI GRGCYC 2 cut(s) 197, 591
Fsp4HI GCNGC 7 cut(s) 162, 165, 228, 311, 852, 882, 894
GlaI GCGC 1 cut(s) 454
GluI GCNGC 7 cut(s) 162, 165, 228, 311, 852, 882, 894
HaeIII GGCC 2 cut(s) 5, 167
HapII CCGG 4 cut(s) 123, 233, 314, 941
HgaI GACGC 1 cut(s) 23
HhaI GCGC 1 cut(s) 455
Hin1II CATG 1 cut(s) 757
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 6 cut(s) 180, 258, 273, 364, 604, 757
HpaII CCGG 4 cut(s) 123, 233, 314, 941
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 7 cut(s) 115, 179, 192, 298, 501, 901, 934
Hpy188III TCNNGA 6 cut(s) 88, 353, 421, 536, 599, 641
Hpy8I GTNNAC 1 cut(s) 150
Hpy99I CGWCG 9 cut(s) 17, 32, 38, 86, 89, 154, 217, 281, 302
HpyAV CCTTC 5 cut(s) 134, 332, 385, 568, 628
HpyCH4III ACNGT 5 cut(s) 59, 245, 302, 664, 975
HpyCH4V TGCA 8 cut(s) 331, 557, 788, 806, 833, 851, 875, 938
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 233, 316, 785, 812, 881, 890
HpyF3I CTNAG 5 cut(s) 237, 413, 438, 585, 966
Hsp92II CATG 1 cut(s) 757
HspAI GCGC 1 cut(s) 453
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 9 cut(s) 37, 154, 187, 417, 532, 637, 657, 697, 869
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 3 cut(s) 235, 316, 778
Lsp1109I GCAGC 5 cut(s) 214, 297, 863, 868, 880
MalI GATC 9 cut(s) 39, 156, 189, 419, 534, 639, 659, 699, 871
MboI GATC 9 cut(s) 37, 154, 187, 417, 532, 637, 657, 697, 869
MboII GAAGA 4 cut(s) 197, 341, 443, 856
MflI RGATCY 3 cut(s) 187, 417, 869
MhlI GDGCHC 2 cut(s) 197, 591
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 3 cut(s) 78, 321, 551
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 2 cut(s) 477, 982
MslI CAYNNNNRTG 1 cut(s) 552
MspI CCGG 4 cut(s) 123, 233, 314, 941
MspR9I CCNGG 2 cut(s) 71, 684
Mva1269I GAATGC 2 cut(s) 455, 559
MvaI CCWGG 2 cut(s) 71, 684
MvnI CGCG 1 cut(s) 453
MwoI GCNNNNNNNGC 7 cut(s) 30, 233, 316, 785, 812, 881, 890
NdeII GATC 9 cut(s) 37, 154, 187, 417, 532, 637, 657, 697, 869
NlaIII CATG 1 cut(s) 757
NlaIV GGNNCC 4 cut(s) 318, 419, 675, 888
NmeAIII GCCGAG 1 cut(s) 345
NspV TTCGAA 1 cut(s) 183
PaqCI CACCTGC 1 cut(s) 778
PciSI GCTCTTC 1 cut(s) 354
PcsI WCGNNNNNNNCGW 3 cut(s) 33, 81, 93
PctI GAATGC 2 cut(s) 455, 559
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 5 cut(s) 180, 258, 273, 604, 757
PflFI GACNNNGTC 1 cut(s) 77
PflMI CCANNNNNTGG 1 cut(s) 683
PkrI GCNGC 7 cut(s) 163, 166, 229, 312, 853, 883, 895
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 591
Psp6I CCWGG 2 cut(s) 69, 682
PspGI CCWGG 2 cut(s) 69, 682
PspN4I GGNNCC 4 cut(s) 318, 419, 675, 888
PspPI GGNCC 2 cut(s) 302, 674
PstI CTGCAG 2 cut(s) 853, 877
PstNI CAGNNNCTG 2 cut(s) 881, 956
PsuI RGATCY 3 cut(s) 187, 417, 869
PsyI GACNNNGTC 1 cut(s) 77
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 552
SacI GAGCTC 1 cut(s) 591
SalI GTCGAC 1 cut(s) 148
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 2 cut(s) 477, 982
SatI GCNGC 7 cut(s) 162, 165, 228, 311, 852, 882, 894
Sau3AI GATC 9 cut(s) 37, 154, 187, 417, 532, 637, 657, 697, 869
Sau96I GGNCC 2 cut(s) 302, 674
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 2 cut(s) 71, 684
SduI GDGCHC 2 cut(s) 197, 591
SfcI CTRYAG 2 cut(s) 849, 873
SfuI TTCGAA 1 cut(s) 183
SinI GGWCC 2 cut(s) 302, 674
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SsiI CCGC 6 cut(s) 126, 135, 161, 164, 746, 884
SstI GAGCTC 1 cut(s) 591
StyD4I CCNGG 2 cut(s) 69, 682
TaaI ACNGT 5 cut(s) 59, 245, 302, 664, 975
TaqI TCGA 7 cut(s) 12, 27, 87, 149, 183, 276, 656
TauI GCSGC 2 cut(s) 164, 167
TfiI GAWTC 5 cut(s) 180, 258, 273, 604, 757
Tru1I TTAA 2 cut(s) 477, 982
Tru9I TTAA 2 cut(s) 477, 982
TscAI CASTG 1 cut(s) 700
TseI GCWGC 5 cut(s) 227, 310, 851, 881, 893
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 1 cut(s) 700
Tth111I GACNNNGTC 1 cut(s) 77
Van91I CCANNNNNTGG 1 cut(s) 683
VpaK11BI GGWCC 2 cut(s) 302, 674
XmiI GTMKAC 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.