FvH4_3g05580

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016008
Physical Location & Seq
Forward (+)
3270478 .. 3271763
1286 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05580.t1

Sequence Viewer

Length: 465 bp
ATGTCAGGCCAGAAGCTAATCGCTACCCACAGAGACGGCGCCGAGATCCATCACGGCGAAGACATATGCAAGCAAAAGTCCCACCAGCTCCTCGACAAGATGCATCTGCCGCGAGGCCTCCTCCCCCTAGACGACCTCGTCGAGTTCGGCTACAACCCCACCTCGGGGTTCGTGTGGCTCAAGCAGAAGAAGCGAAAGGAGCACCGCTTCCACGCCGTGGGACGCACGGTTTCATACGACGCCGAGGTCATGGGGTTCGTGGAGGAGCATCGGATGAGGCGCCTCAGCGGGGTCAAGAGTAAGGAGATTCTGATCTGGGTCTCCATTTCTGATATCTATGTGGATCCGAGTACAGATCCTGATAAGATCACGTTCGCCAACGGCTCCGGAATCTCGAGGTCCTTCCCCATCACGGCGTTTCAGCTTGAAGGTGGAGATGGAAGTGGTAGTAATGAGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

155

Amino Acids

17.32

Weight (kDa)

7.91

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 141 9.5e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016008)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46230
fragaria_vesca FvH4_3g05580
malus_domestica MD05G1284100.v1.1 MD10G1262400.v1.1
prunus_persica Prupe.4G080300_v2.0.a1
pyrus_communis pycom05g26320
rosa_chinensis RchiOBHm_Chr5g0014081
rosa_laevigata RLG00000032080
rosa_multiflora Rmu_sc0007767.1_g000010
rosa_roxburghii Rroxscaffold_1G00062540
rosa_rugosa Rorug05G0015500
rosa_samantha Rh5AG109500 Rh5BG106300 Rh5CG118000
rosa_wichuraiana Rw5G009560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 137, 245
AccB1I GGYRCC 2 cut(s) 38, 279
AccB7I CCANNNNNTGG 1 cut(s) 217
AccII CGCG 1 cut(s) 112
AccIII TCCGGA 1 cut(s) 386
AciI CCGC 3 cut(s) 110, 205, 288
AclWI GGATC 4 cut(s) 40, 338, 350, 351
AcyI GRCGYC 3 cut(s) 39, 240, 280
AdeI CACNNNGTG 1 cut(s) 217
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 6 cut(s) 163, 164, 165, 217, 289, 412
AgsI TTSAA 1 cut(s) 428
AluBI AGCT 3 cut(s) 16, 88, 424
AluI AGCT 3 cut(s) 16, 88, 424
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 27, 325
AlwI GGATC 4 cut(s) 40, 338, 350, 351
AlwNI CAGNNNCTG 1 cut(s) 359
Ama87I CYCGRG 2 cut(s) 163, 394
Aor13HI TCCGGA 1 cut(s) 386
AoxI GGCC 2 cut(s) 7, 115
AspLEI GCGC 2 cut(s) 41, 282
AspS9I GGNCC 1 cut(s) 399
AvaI CYCGRG 2 cut(s) 163, 394
AvaII GGWCC 1 cut(s) 399
BamHI GGATCC 1 cut(s) 343
BanI GGYRCC 2 cut(s) 38, 279
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 204
BbvCI CCTCAGC 1 cut(s) 284
BccI CCATC 3 cut(s) 57, 416, 431
BceAI ACGGC 5 cut(s) 52, 70, 200, 397, 429
BcoDI GTCTC 2 cut(s) 27, 325
BfaI CTAG 1 cut(s) 128
BfoI RGCGCY 2 cut(s) 42, 283
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 399
BmeT110I CYCGRG 2 cut(s) 163, 394
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 4 cut(s) 40, 281, 345, 385
BmsI GCATC 3 cut(s) 90, 112, 277
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu10I CCTNAGC 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 311
BsaHI GRCGYC 3 cut(s) 39, 240, 280
BsaI GGTCTC 1 cut(s) 325
BsaJI CCNNGG 3 cut(s) 162, 216, 243
BsaWI WCCGGW 1 cut(s) 386
Bsc4I CCNNNNNNNGG 6 cut(s) 163, 164, 165, 217, 289, 412
Bse8I GATNNNNATC 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 386
BseDI CCNNGG 3 cut(s) 162, 216, 243
BseGI GGATG 1 cut(s) 279
BseJI GATNNNNATC 1 cut(s) 311
BseLI CCNNNNNNNGG 6 cut(s) 163, 164, 165, 217, 289, 412
BseMII CTCAG 1 cut(s) 298
BseRI GAGGAG 3 cut(s) 80, 110, 278
Bsh1236I CGCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 9, 117
BshNI GGYRCC 2 cut(s) 38, 279
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 2 cut(s) 163, 394
BsiSI CCGG 1 cut(s) 387
BslFI GGGAC 2 cut(s) 64, 234
BslI CCNNNNNNNGG 6 cut(s) 163, 164, 165, 217, 289, 412
BsmAI GTCTC 2 cut(s) 27, 325
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 64, 234
BsnI GGCC 2 cut(s) 9, 117
Bso31I GGTCTC 1 cut(s) 325
BsoBI CYCGRG 2 cut(s) 163, 394
Bsp1286I GDGCHC 1 cut(s) 204
Bsp13I TCCGGA 1 cut(s) 386
Bsp143I GATC 5 cut(s) 45, 312, 343, 355, 366
BspACI CCGC 3 cut(s) 110, 205, 288
BspANI GGCC 2 cut(s) 9, 117
BspCNI CTCAG 1 cut(s) 297
BspEI TCCGGA 1 cut(s) 386
BspFNI CGCG 1 cut(s) 112
BspLI GGNNCC 4 cut(s) 40, 281, 345, 385
BspPI GGATC 4 cut(s) 40, 338, 350, 351
BspT107I GGYRCC 2 cut(s) 38, 279
BspTNI GGTCTC 1 cut(s) 325
BssECI CCNNGG 3 cut(s) 162, 216, 243
BssMI GATC 5 cut(s) 45, 312, 343, 355, 366
BssNI GRCGYC 3 cut(s) 39, 240, 280
Bst4CI ACNGT 1 cut(s) 229
BstACI GRCGYC 3 cut(s) 39, 240, 280
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 1 cut(s) 284
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 1 cut(s) 112
BstH2I RGCGCY 2 cut(s) 42, 283
BstHHI GCGC 2 cut(s) 41, 282
BstKTI GATC 5 cut(s) 48, 315, 346, 358, 369
BstMAI GTCTC 2 cut(s) 27, 325
BstMBI GATC 5 cut(s) 45, 312, 343, 355, 366
BstMWI GCNNNNNNNGC 3 cut(s) 109, 190, 199
BstUI CGCG 1 cut(s) 112
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 3 cut(s) 45, 343, 355
BstYI RGATCY 3 cut(s) 45, 343, 355
BsuRI GGCC 2 cut(s) 9, 117
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 71
CaiI CAGNNNCTG 1 cut(s) 359
CfoI GCGC 2 cut(s) 41, 282
Cfr13I GGNCC 1 cut(s) 399
CseI GACGC 2 cut(s) 231, 248
Csp6I GTAC 1 cut(s) 351
CviAII CATG 1 cut(s) 250
CviJI RGCY 8 cut(s) 9, 16, 88, 117, 150, 178, 384, 424
CviKI_1 RGCY 8 cut(s) 9, 16, 88, 117, 150, 178, 384, 424
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 1 cut(s) 284
DinI GGCGCC 2 cut(s) 40, 281
DpnI GATC 5 cut(s) 47, 314, 345, 357, 368
DpnII GATC 5 cut(s) 45, 312, 343, 355, 366
DraIII CACNNNGTG 1 cut(s) 217
DrdI GACNNNNNNGTC 2 cut(s) 137, 245
DseDI GACNNNNNNGTC 2 cut(s) 137, 245
Eco147I AGGCCT 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 325
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 399
Eco88I CYCGRG 2 cut(s) 163, 394
EcoO109I RGGNCCY 1 cut(s) 399
EcoRV GATATC 1 cut(s) 334
EcoT22I ATGCAT 1 cut(s) 105
EgeI GGCGCC 2 cut(s) 40, 281
EheI GGCGCC 2 cut(s) 40, 281
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 1 cut(s) 253
FaiI YATR 5 cut(s) 65, 67, 235, 251, 339
FaqI GGGAC 2 cut(s) 64, 234
FatI CATG 1 cut(s) 249
FauI CCCGC 1 cut(s) 281
FauNDI CATATG 1 cut(s) 65
Fnu4HI GCNGC 1 cut(s) 110
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 110
FspBI CTAG 1 cut(s) 128
GlaI GCGC 2 cut(s) 40, 281
GluI GCNGC 1 cut(s) 110
HaeII RGCGCY 2 cut(s) 42, 283
HaeIII GGCC 2 cut(s) 9, 117
HapII CCGG 1 cut(s) 387
HgaI GACGC 2 cut(s) 231, 248
HhaI GCGC 2 cut(s) 41, 282
Hin1I GRCGYC 3 cut(s) 39, 240, 280
Hin1II CATG 1 cut(s) 253
Hin6I GCGC 2 cut(s) 39, 280
HinP1I GCGC 2 cut(s) 39, 280
HinfI GANTC 2 cut(s) 307, 390
HpaII CCGG 1 cut(s) 387
Hpy188I TCNGA 4 cut(s) 273, 312, 331, 348
Hpy188III TCNNGA 4 cut(s) 295, 359, 387, 394
Hpy99I CGWCG 2 cut(s) 143, 242
HpyAV CCTTC 2 cut(s) 412, 422
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 2 cut(s) 69, 103
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 190, 199
HpyF3I CTNAG 1 cut(s) 284
HpySE526I ACGT 1 cut(s) 371
Hsp92I GRCGYC 3 cut(s) 39, 240, 280
Hsp92II CATG 1 cut(s) 253
HspAI GCGC 2 cut(s) 39, 280
KasI GGCGCC 2 cut(s) 38, 279
Kpn2I TCCGGA 1 cut(s) 386
Kzo9I GATC 5 cut(s) 45, 312, 343, 355, 366
LmnI GCTCC 4 cut(s) 93, 199, 265, 389
LpnPI CCDG 5 cut(s) 23, 98, 301, 372, 400
LweI GCATC 3 cut(s) 90, 112, 277
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 371
MalI GATC 5 cut(s) 47, 314, 345, 357, 368
MboI GATC 5 cut(s) 45, 312, 343, 355, 366
MboII GAAGA 2 cut(s) 71, 199
MflI RGATCY 3 cut(s) 45, 343, 355
MhlI GDGCHC 1 cut(s) 204
Mly113I GGCGCC 2 cut(s) 39, 280
Mph1103I ATGCAT 1 cut(s) 105
MroI TCCGGA 1 cut(s) 386
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 1 cut(s) 387
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 3 cut(s) 109, 190, 199
NarI GGCGCC 2 cut(s) 39, 280
NdeI CATATG 1 cut(s) 65
NdeII GATC 5 cut(s) 45, 312, 343, 355, 366
NlaIII CATG 1 cut(s) 253
NlaIV GGNNCC 4 cut(s) 40, 281, 345, 385
NmeAIII GCCGAG 2 cut(s) 67, 268
NsiI ATGCAT 1 cut(s) 105
PaeR7I CTCGAG 1 cut(s) 394
PceI AGGCCT 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PfeI GAWTC 2 cut(s) 307, 390
PflFI GACNNNGTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 1 cut(s) 111
PluTI GGCGCC 2 cut(s) 42, 283
PpuMI RGGWCCY 1 cut(s) 399
Psp5II RGGWCCY 1 cut(s) 399
PspN4I GGNNCC 4 cut(s) 40, 281, 345, 385
PspPI GGNCC 1 cut(s) 399
PspPPI RGGWCCY 1 cut(s) 399
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 3 cut(s) 45, 343, 355
PsyI GACNNNGTC 1 cut(s) 137
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 5 cut(s) 45, 312, 343, 355, 366
Sau96I GGNCC 1 cut(s) 399
SduI GDGCHC 1 cut(s) 204
SetI ASST 9 cut(s) 18, 90, 138, 164, 249, 374, 401, 426, 433
SfaNI GCATC 3 cut(s) 90, 112, 277
SfoI GGCGCC 2 cut(s) 40, 281
Sfr274I CTCGAG 1 cut(s) 394
SinI GGWCC 1 cut(s) 399
SlaI CTCGAG 1 cut(s) 394
SmlI CTYRAG 2 cut(s) 179, 394
SmoI CTYRAG 2 cut(s) 179, 394
SseBI AGGCCT 1 cut(s) 117
SsiI CCGC 3 cut(s) 110, 205, 288
SspDI GGCGCC 2 cut(s) 38, 279
SspMI CTAG 1 cut(s) 128
StuI AGGCCT 1 cut(s) 117
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 374
TaqI TCGA 3 cut(s) 93, 141, 395
TatI WGTACW 1 cut(s) 350
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 2 cut(s) 307, 390
TspDTI ATGAA 1 cut(s) 222
Tth111I GACNNNGTC 1 cut(s) 137
Van91I CCANNNNNTGG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 399
XhoI CTCGAG 1 cut(s) 394
XspI CTAG 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.