MD05G1284100.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016008
Physical Location & Seq
Forward (+)
41811701 .. 41812165
465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1284100.v1.1.491

Sequence Viewer

Length: 465 bp
ATGTCAGCCCAACAAATCGCCGGCCACAGAGAGGGCGCCGATATCTACCACGGCGAAGCCCTATGCAGACAGAAGTTCCACGAGCTGCTCGACAAAATGTGCCTGCCACGTGGCCTCCTCCCCTTGGACGACGTCGTCGAGTTCGGCTACAACTCCACCTCGGGCTTCGTCTGGCTCAAGCAGAAGAAGAGAAAAGAGCACCGCTTCCGCGCCATTTCCAAAACGGTGTCGTACGACACGGAGGTGATGGGGTTCCTGGAGGAGCACCGGATGCGGCGGCTCTCCGGGGTCAAAAGCAAAGAGCTTCTGGTTTGGGTCTCCATTTCGGACATCTACGTGGACCCTAATTCGGATCTGAATAAGATCACGTTCGCTAACGGCTCCGGAATCTCCAGGTCGTTCCCCGTCACGGCGTTTGGGCTTGAAGGAGAAGAGAAAAACGGTGACGGCGATGGGAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

155

Amino Acids

17.42

Weight (kDa)

7.8

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 27 - 139 6.6e-31 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016008)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46230
fragaria_vesca FvH4_3g05580
malus_domestica MD05G1284100.v1.1 MD10G1262400.v1.1
prunus_persica Prupe.4G080300_v2.0.a1
pyrus_communis pycom05g26320
rosa_chinensis RchiOBHm_Chr5g0014081
rosa_laevigata RLG00000032080
rosa_multiflora Rmu_sc0007767.1_g000010
rosa_roxburghii Rroxscaffold_1G00062540
rosa_rugosa Rorug05G0015500
rosa_samantha Rh5AG109500 Rh5BG106300 Rh5CG118000
rosa_wichuraiana Rw5G009560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 35
AccII CGCG 1 cut(s) 210
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 4 cut(s) 202, 208, 274, 277
AclWI GGATC 1 cut(s) 360
AcoI YGGCCR 1 cut(s) 22
AcvI CACGTG 1 cut(s) 110
AcyI GRCGYC 2 cut(s) 36, 132
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 4 cut(s) 31, 124, 349, 409
AgsI TTSAA 1 cut(s) 425
AjnI CCWGG 2 cut(s) 255, 392
AleI CACNNNNGTG 1 cut(s) 242
AluBI AGCT 2 cut(s) 85, 304
AluI AGCT 2 cut(s) 85, 304
Alw21I GWGCWC 2 cut(s) 201, 267
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 1 cut(s) 360
Ama87I CYCGRG 1 cut(s) 160
Aor13HI TCCGGA 1 cut(s) 383
AoxI GGCC 2 cut(s) 22, 112
ApeKI GCWGC 1 cut(s) 85
AspLEI GCGC 2 cut(s) 38, 212
AspS9I GGNCC 1 cut(s) 340
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 2 cut(s) 256, 455
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 1 cut(s) 340
BanI GGYRCC 1 cut(s) 35
BauI CACGAG 1 cut(s) 80
BbrPI CACGTG 1 cut(s) 110
Bbv12I GWGCWC 2 cut(s) 201, 267
BbvI GCAGC 1 cut(s) 72
BccI CCATC 2 cut(s) 241, 446
BceAI ACGGC 4 cut(s) 67, 394, 426, 463
BciT130I CCWGG 2 cut(s) 257, 394
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 322
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 3 cut(s) 86, 275, 278
BlsI GCNGC 3 cut(s) 87, 276, 279
Bme1390I CCNGG 3 cut(s) 257, 286, 394
Bme18I GGWCC 1 cut(s) 340
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 340
BmiI GGNNCC 4 cut(s) 37, 254, 342, 382
BmrFI CCNGG 3 cut(s) 257, 286, 394
BmsI GCATC 1 cut(s) 261
BpmI CTGGAG 2 cut(s) 278, 376
BpuEI CTTGAG 1 cut(s) 161
BpuMI CCSGG 1 cut(s) 286
BsaAI YACGTR 2 cut(s) 110, 337
BsaHI GRCGYC 2 cut(s) 36, 132
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 4 cut(s) 49, 123, 159, 285
BsaWI WCCGGW 2 cut(s) 267, 383
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 124, 349, 409
Bse118I RCCGGY 1 cut(s) 20
BseAI TCCGGA 1 cut(s) 383
BseBI CCWGG 2 cut(s) 257, 394
BseDI CCNNGG 4 cut(s) 49, 123, 159, 285
BseGI GGATG 1 cut(s) 276
BseLI CCNNNNNNNGG 4 cut(s) 31, 124, 349, 409
BseRI GAGGAG 2 cut(s) 107, 275
BseXI GCAGC 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 24, 114
BshNI GGYRCC 1 cut(s) 35
BsiHKAI GWGCWC 2 cut(s) 201, 267
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 4 cut(s) 21, 268, 285, 384
BsiWI CGTACG 1 cut(s) 231
BslI CCNNNNNNNGG 4 cut(s) 31, 124, 349, 409
BsmAI GTCTC 1 cut(s) 322
BsnI GGCC 2 cut(s) 24, 114
Bso31I GGTCTC 1 cut(s) 322
BsoBI CYCGRG 1 cut(s) 160
Bsp1286I GDGCHC 2 cut(s) 201, 267
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 2 cut(s) 352, 363
BspACI CCGC 4 cut(s) 202, 208, 274, 277
BspANI GGCC 2 cut(s) 24, 114
BspEI TCCGGA 1 cut(s) 383
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 4 cut(s) 37, 254, 342, 382
BspPI GGATC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 4 cut(s) 49, 123, 159, 285
BssMI GATC 2 cut(s) 352, 363
BssNI GRCGYC 2 cut(s) 36, 132
BssSI CACGAG 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 2 cut(s) 257, 394
Bst4CI ACNGT 2 cut(s) 226, 443
Bst6I CTCTTC 2 cut(s) 182, 426
BstACI GRCGYC 2 cut(s) 36, 132
BstAPI GCANNNNNTGC 1 cut(s) 271
BstBAI YACGTR 2 cut(s) 110, 337
BstC8I GCNNGC 2 cut(s) 22, 104
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 276
BstFNI CGCG 1 cut(s) 210
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 212
BstKTI GATC 2 cut(s) 355, 366
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 2 cut(s) 352, 363
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstNI CCWGG 2 cut(s) 257, 394
BstSCI CCNGG 3 cut(s) 255, 284, 392
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 2 cut(s) 24, 114
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 276
Cac8I GCNNGC 2 cut(s) 22, 104
CfoI GCGC 2 cut(s) 38, 212
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 340
Csp6I GTAC 1 cut(s) 232
CviQI GTAC 1 cut(s) 232
DinI GGCGCC 1 cut(s) 37
DpnI GATC 2 cut(s) 354, 365
DpnII GATC 2 cut(s) 352, 363
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 22
Eam1104I CTCTTC 2 cut(s) 182, 426
EarI CTCTTC 2 cut(s) 182, 426
Eco130I CCWWGG 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 322
Eco32I GATATC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 340
Eco72I CACGTG 1 cut(s) 110
Eco88I CYCGRG 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 255, 392
EcoRV GATATC 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 123
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 123
FaiI YATR 1 cut(s) 64
Fnu4HI GCNGC 3 cut(s) 86, 275, 278
FokI GGATG 1 cut(s) 283
Fsp4HI GCNGC 3 cut(s) 86, 275, 278
GlaI GCGC 2 cut(s) 37, 211
GluI GCNGC 3 cut(s) 86, 275, 278
GsuI CTGGAG 2 cut(s) 278, 376
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 2 cut(s) 24, 114
HapII CCGG 4 cut(s) 21, 268, 285, 384
HhaI GCGC 2 cut(s) 38, 212
Hin1I GRCGYC 2 cut(s) 36, 132
Hin6I GCGC 2 cut(s) 36, 210
HinP1I GCGC 2 cut(s) 36, 210
HinfI GANTC 1 cut(s) 387
HpaII CCGG 4 cut(s) 21, 268, 285, 384
HphI GGTGA 2 cut(s) 256, 455
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 3 cut(s) 328, 352, 357
Hpy188III TCNNGA 1 cut(s) 384
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 3 cut(s) 134, 137, 140
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 2 cut(s) 226, 443
HpyCH4IV ACGT 4 cut(s) 109, 132, 336, 368
HpyCH4V TGCA 1 cut(s) 66
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
HpySE526I ACGT 4 cut(s) 109, 132, 336, 368
Hsp92I GRCGYC 2 cut(s) 36, 132
HspAI GCGC 2 cut(s) 36, 210
KasI GGCGCC 1 cut(s) 35
Kpn2I TCCGGA 1 cut(s) 383
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 2 cut(s) 352, 363
LmnI GCTCC 2 cut(s) 262, 386
Lsp1109I GCAGC 1 cut(s) 72
LweI GCATC 1 cut(s) 261
MaeII ACGT 4 cut(s) 109, 132, 336, 368
MaeIII GTNAC 2 cut(s) 406, 443
MalI GATC 2 cut(s) 354, 365
MboI GATC 2 cut(s) 352, 363
MboII GAAGA 3 cut(s) 196, 199, 443
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 2 cut(s) 201, 267
MluCI AATT 1 cut(s) 346
Mly113I GGCGCC 1 cut(s) 36
MnlI CCTC 6 cut(s) 25, 125, 128, 169, 235, 253
MroI TCCGGA 1 cut(s) 383
MroNI GCCGGC 1 cut(s) 20
MslI CAYNNNNRTG 2 cut(s) 242, 335
MspI CCGG 4 cut(s) 21, 268, 285, 384
MspR9I CCNGG 3 cut(s) 257, 286, 394
MvaI CCWGG 2 cut(s) 257, 394
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 271
NaeI GCCGGC 1 cut(s) 22
NarI GGCGCC 1 cut(s) 36
NciI CCSGG 1 cut(s) 286
NdeII GATC 2 cut(s) 352, 363
NgoMIV GCCGGC 1 cut(s) 20
NlaIV GGNNCC 4 cut(s) 37, 254, 342, 382
NmuCI GTSAC 2 cut(s) 406, 443
OliI CACNNNNGTG 1 cut(s) 242
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 135, 447
PdiI GCCGGC 1 cut(s) 22
PfeI GAWTC 1 cut(s) 387
Pfl23II CGTACG 1 cut(s) 231
PflFI GACNNNGTC 2 cut(s) 131, 134
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 3 cut(s) 87, 276, 279
PluTI GGCGCC 1 cut(s) 39
PmaCI CACGTG 1 cut(s) 110
PmlI CACGTG 1 cut(s) 110
Ppu21I YACGTR 2 cut(s) 110, 337
Psp6I CCWGG 2 cut(s) 255, 392
PspCI CACGTG 1 cut(s) 110
PspGI CCWGG 2 cut(s) 255, 392
PspLI CGTACG 1 cut(s) 231
PspN4I GGNNCC 4 cut(s) 37, 254, 342, 382
PspPI GGNCC 1 cut(s) 340
PsuI RGATCY 1 cut(s) 352
PsyI GACNNNGTC 2 cut(s) 131, 134
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 2 cut(s) 242, 335
SatI GCNGC 3 cut(s) 86, 275, 278
Sau3AI GATC 2 cut(s) 352, 363
Sau96I GGNCC 1 cut(s) 340
ScrFI CCNGG 3 cut(s) 257, 286, 394
SduI GDGCHC 2 cut(s) 201, 267
SetI ASST 9 cut(s) 87, 112, 135, 161, 246, 306, 339, 371, 398
SfaNI GCATC 1 cut(s) 261
SfoI GGCGCC 1 cut(s) 37
SinI GGWCC 1 cut(s) 340
SmiMI CAYNNNNRTG 2 cut(s) 242, 335
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 1 cut(s) 346
SsiI CCGC 4 cut(s) 202, 208, 274, 277
SspDI GGCGCC 1 cut(s) 35
StyD4I CCNGG 3 cut(s) 255, 284, 392
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 226, 443
TaiI ACGT 4 cut(s) 112, 135, 339, 371
TaqI TCGA 2 cut(s) 90, 138
TasI AATT 1 cut(s) 346
TauI GCSGC 2 cut(s) 277, 280
TfiI GAWTC 1 cut(s) 387
TseFI GTSAC 2 cut(s) 406, 443
TseI GCWGC 1 cut(s) 85
Tsp45I GTSAC 2 cut(s) 406, 443
TspGWI ACGGA 1 cut(s) 254
Tth111I GACNNNGTC 2 cut(s) 131, 134
VpaK11BI GGWCC 1 cut(s) 340
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.